Abstract During the past decades, an intense process of policy circulation of LGBTI+ policies has taken place in Brazil. Since those policies had weak induction capacities in the federal level, how to explain their circulation between levels and units of the Brazilian federation? To answer this question, this article analyses the circulation trajectories of the “LGBTI+ reference centers” and the “citizenship triplet” among five observation units using data from official documents and interviews. Results show that “multilevel activism” was crucial for the circulation of those policies, through the construction of informal interaction networks that integrate levels, the foundation of multilevel organizations, the action within arenas that gather actors from multiple levels and the displacement and multiple filiations of actors between levels. The article argues for the fertility of a “multilevel approach” to the relations between social movements and public policies. decades LGBTI Brazil level federation question “LGBTI centers citizenship triplet interviews activism organizations approach

Résumé Au cours des dernières décennies, on observe une intense circulation de politiques en faveur des LGBTI+ au Brésil. Étant donné que ces politiques ont été marquées par de faibles capacités d’induction du gouvernement fédéral, comment expliquer leur circulation entre les niveaux et les unités de la fédération ? Pour répondre à cette question, l’article analyse la trajectoire de circulation des centres de référence LGBTI+ et du “tripode de la citoyenneté” dans cinq unités d’observation à partir de données d’entretiens et d’analyses documentaires. Les résultats indiquent que « l’activisme multiniveau » a été central pour la circulation de ces politiques, à travers la construction de réseaux informels d’interaction qui intègrent les niveaux, la formation d’organisations multiniveaux, l’action dans des arènes réunissant des acteurs de multiples niveaux et le déplacement et l’appartenance multiple des acteurs entre les niveaux. Ainsi, l’article défend la fécondité d’un « regard multiniveau » sur les relations entre les mouvements sociaux et les politiques publiques. décennies LGBTI Brésil dinduction d induction fédéral question larticle l article tripode citoyenneté dobservation observation dentretiens entretiens danalyses analyses documentaires lactivisme activisme dinteraction interaction dorganisations organisations multiniveaux laction action lappartenance appartenance Ainsi dun un publiques

Resumo Nas últimas décadas, observa-se uma intensa circulação de políticas para LGBTI+ no Brasil. Uma vez que tais políticas foram marcadas pela fraca capacidade de indução do Governo Federal, como explicar sua circulação entre níveis e unidades da federação? Para responder essa pergunta, o artigo analisa a trajetória de circulação dos centros de referência LGBTI+ e do “tripé da cidadania” em cinco unidades de observação a partir de dados de entrevistas e de análise documental. Os resultados indicam que o “ativismo multinível” foi fundamental para a circulação dessas políticas por meio: da construção de redes informais de interação que integram níveis; da formação de organizações multinível; da atuação em arenas que reúnem atores oriundos de múltiplos níveis; e do deslocamento e múltiplo pertencimento de atores entre níveis. O artigo defende, assim, a fertilidade de um “olhar multinível” para as relações entre movimentos sociais e políticas públicas. décadas observase observa se LGBTI Brasil Federal federação pergunta tripé cidadania documental ativismo multinível meio defende assim olhar públicas

Resumen En las últimas décadas, ha habido una intensa circulación de políticas LGBTI+ en Brasil. Dado que estas políticas han estado marcadas por la escasa capacidad de inducción del gobierno federal, ¿cómo explicar su circulación entre niveles y unidades de la federación? Para responder a esta pregunta, el artículo analiza la circulación de los centros de referencia LGBTI+ y el “trípode de la ciudadanía” en cinco unidades de observación a partir de datos de entrevistas y análisis documentales. Los resultados indican que el “activismo multinivel” fue fundamental para la circulación de estas políticas, a través de la construcción de redes de interacción informales que integran niveles, la formación de organizaciones multinivel, la acción en arenas que reúnen a actores de múltiples niveles y el desplazamiento y la pertenencia múltiple de actores entre niveles. El artículo defiende así la utilidad de una “visión multinivel” de la relación entre los movimientos sociales y las políticas públicas. décadas LGBTI Brasil federal cómo federación pregunta trípode ciudadanía documentales activismo multinivel visión públicas

Abstract Introduction. Cocoa cultivation in tropical areas is a commercially viable agroecological alternative that allows for productive diversification and sustainable soil management through the use of organic fertilization. Objective. To evaluate the development of four cocoa varieties (INIFAP 4, INIFAP 8, INIFAP 9, and Almendra Blanca) under the climatic conditions of the Úrsulo Galván region, Veracruz, Mexico. Materials and methods. An experiment was conducted between winter (December 2021) and summer (June 2022), using a completely randomized design with four treatments and ten replications. Conventional agronomic management was employed, with the addition of 10 kg of compost per tree, followed by 3 kg of vermicompost. At four months into the study, 50 g of urea, 50 g of ammonium diphosphate, and 50 g of potassium chloride were incorporated. The study included analysis of variance and Tukey’s tests (α 0.05). Results. Statistical equality was found for height, diameter, and number of branches. The cocoa’s response to temperature, precipitation, relative humidity, and wind showed that these factors influenced the expression of its productive potential, as well as its morphology and yield, without adapting to the climatic conditions under study. Conclusion. The development of the studied varieties was homogeneous based on the climatic conditions of the Ursulo Galvan region, with the Almendra Blanca variety standing out.

Resumen Introducción. El cultivo del cacao en las zonas tropicales es una alternativa comercial de interés agroecológico, que permite la diversificación productiva y el manejo sustentable de los suelos al emplear abonado orgánico. Objetivo. Evaluar el desarrollo de cuatro variedades de cacao (INIFAP 4, INIFAP 8, INIFAP 9 y Almendra Blanca) en las condiciones climatológicas de la región de Úrsulo Galván, Veracruz, México. Materiales y métodos. Se realizó un experimento, entre invierno (diciembre 2021) y verano (junio 2022), bajo un diseño completamente al azar, distribuido en cuatro tratamientos y diez repeticiones. El manejo agronómico fue convencional, se agregaron 10 kg de composta por árbol, seguidos de 3 kg de vermicompost. A los cuatro meses del estudio se incorporaron 50 g de urea, 50 g de difosfato de amonio y 50 g de cloruro de potasio. El estudio incluyó análisis de varianza y pruebas de Tukey (α 0,05). Resultados. Se encontró igualdad estadística para altura, diámetro y número de ramas. La respuesta del cacao a temperatura, precipitación, humedad relativa y viento, demostró que afectaron la expresión de su potencial productivo, así como su morfología y rendimiento, no adaptándose a las condiciones climáticas en estudio. Conclusión. El desarrollo de las variedades en estudio fue homogéneo, con base en las condiciones climatológicas de la región Úrsulo Galván, destacándose la variedad Almendra Blanca.

Abstract Introduction. The soil and climatic conditions of Ecuador are ideal for rice cultivation, yields are higher than the world average, but agronomic management leads to an increase of cadmium in the soil, which can reach the grain by translocation, which affects its safety. Objetive. To evaluate the absorption of cadmium through the omission of macronutrients technique in six soils of Ecuador and its effect on the vegetative development of rice crops. Materials and methods. The trial was carried out at the Pichilingue Tropical Experimental Station of the National Institute of Agricultural Research, Ecuador, during 2018. Nine treatments were studied: 1) Without fertilization and without Cd (control 1), 2) without fertilization and with Cd (Control 2), 3) Complete fertilization and six omission treatments of one nutrient at a time, a completely randomized block design of experiments, divided plots and three repetitions was used. The variables evaluated were those related to the production of dry matter in the root and aerial part. For the comparison between means, the Tukey test was used (p<0.05). Results. Nutrients omissions (N and P) led to reduced Cd absorption, which decreased dry matter production, particularly in very acidic or alkaline soils such as in Sucumbíos (pH 4) and Guayas (pH 7, 9), respectively. Conclusions. The absorption of cadmium in rice plants was reduced to a greater extent with the omission of the macronutrients N and P, mainly in the soils of Sucumbíos, Los Ríos, El Oro and Manabí, the nutrient omission technique also affecting the production of dry matter and yields, also observing that a pH below 5 or higher than 7.9 affects the absorption of cadmium in the soils studied.

Resumen Introducción. Las condiciones edafoclimáticas del Ecuador son ideales para el cultivo del arroz, los rendimientos son mayores al promedio mundial, pero su manejo agronómico conlleva a un aumento de cadmio en el suelo, que puede llegar al grano por translocación, lo cual afectan su inocuidad. Objetivo. Evaluar la absorción de cadmio mediante la técnica de omisión de macronutrientes en seis suelos del Ecuador y su efecto sobre el desarrollo vegetativo del cultivo de arroz. Materiales y métodos. El ensayo se realizó en la Estación Experimental Tropical Pichilingue del Instituto Nacional de Investigaciones Agropecuarias, Ecuador, durante el año 2018. Se estudiaron nueve tratamientos 1) Sin fertilización y sin Cd (testigo 1), 2) sin fertilización y con Cd (Testigo 2), 3) Fertilización completa y seis tratamientos de omisión de un nutriente a la vez, se usó un diseño de experimentos de bloques completamente al azar, parcelas divididas y tres repeticiones. Las variables evaluadas fueron las relacionadas a la producción de materia seca en raíz y parte aérea. Para la comparación entre medias, se utilizó la prueba de Tukey (p<0,05). Resultados. Las omisiones de los nutrientes (N y P) llevaron a la reducción de la absorción del Cd, que disminuyó la producción de materia seca, en particular en los suelos muy ácidos o alcalinos como en Sucumbíos (pH 4) y Guayas (pH 7,9), respectivamente. Conclusiones. La absorción de cadmio en plantas de arroz se redujo en mayor medida con la omisión de los macronutrientes N y P, principalmente en los suelos de Sucumbíos, Los Ríos, El Oro y Manabí, afectando también la omisión de nutrientes a la producción de materia seca y los rendimientos, observándose además que un pH por debajo de 5 o superior a 7,9 afecta la absorción de cadmio en los suelos estudiados

Summary: The state of Chiapas presents degradation processes of dif ferent kinds, as a consequence of the current established agricultural model. It is estimated that, in the near future, the surface of the state of Chiapas will present more than 55% degradation, generating changes in the physical, chemical and biological properties of the soil, af fecting the quality of life of human beings, mainly due to scarcity of food. The objective of this research was to evaluate the quality of the soils in three municipalities of the central depression of Chiapas, Mexico. The analysis were interpreted taking NOM-021-SEMARNAT-2000 as a reference and were classified according to the world reference base (WRB), version 2014. For the evaluation of soil quality, the sustainable use of land subindex (SUSS). 144 soil samples corresponding to 21 profiles were analyzed. Of the 21 profiles studied, 17 present poor soil qualities, two a sensitive quality, one acceptable quality and one with good quality. Only two soil groups presented good fertility and are classified as Vertisols and Phaeozems, which present good quality and capacity to maintain sustainable agricultural production in this region.

Resumen: El estado de Chiapas presenta procesos de degradación de diferente índole, como consecuencia del actual modelo agrícola establecido. Se estima que, en el futuro cercano, la superficie del estado de Chiapas presente más de 55% de degradación, generando modificaciones en las propiedades físicas, químicas y biológicas del suelo; afectando la calidad de vida de los seres humanos, principalmente por escasez de alimentos. El objetivo de esta investigación fue evaluar la calidad de los suelos en tres municipios de la depresión central de Chiapas, México. Los análisis se interpretaron tomando como referencia la NOM-021-SEMARNAT-2000 y se clasificaron de acuerdo con la world reference base (WRB), versión 2014, para la evaluación de calidad del suelo se utilizó el subíndice de uso sustentable del suelo (SUSS). Se analizaron 144 muestras de suelo correspondientes a 21 perfiles. De los 21 perfiles estudiados, 17 presentan calidad de suelo pobre, dos calidades sensibles, uno calidad aceptable y uno con calidad buena. Solo dos Grupos de suelo presentaron buena fertilidad y son clasificados como Vertisols y Phaeozems, los cuales presentan buena calidad y capacidad para mantener producción agrícola sostenible en esta región.

Summary: Excessive use of chlorpyrifos affects the quality of agricultural soils causing negative effects on the safety of harvested food. The behavior of chlorpyrifos residues depends on soil properties, especially organic matter. Our aim in this research was to determine the effect of the application of vermicompost on the residual and dissipation of chlorpyrifos in an entisol soil of semi-arid climate with variations in its edaphic fertility. Four treatments were studied. T1: 7.5% vermicompost (V) and 92.5% soil (S); T2: 5% V and 95% S; T3: 2.5% V and 97.5% S; T4: 0% V and 100% S. The treatments were subjected to a seven-day incubation period. Subsequently, a dose of chlorpyrifos of 1.5 mg kg-1 as active ingredient was applied once for each treatment. A completely randomized design was used with three replicates for each treatment, forming 12 experimental units. The analytical determination of chlorpyrifos was carried out by high performance liquid chromatography (HPLC). The evaluations were carried out 1.7, 14, 28, 42, 70 and 98 days after the application of chlorpyrifos. The results showed that the incorporation of vermicompost to the soil at increasing levels promoted a decrease in the residual concentration of chlorpyrifos and facilitated its gradual dissipation. The chlorpyrifos dissipation curves correspond to a first order logarithmic equation where the effects of the treatments caused greater dissipation of chlorpyrifos as the evaluation period elapsed. At 98 days of evaluation, T1 registered lower residuality (0.786 m kg-1), higher daily dissipation rate (0.008) and lower half-life of chlorpyrifos (86.64 days). At the end of the research period, edaphic levels of P, K, EC and CEC increased while OM, N and pH decreased after vermicompost application.

Resumen: El uso excesivo de clorpirifos afecta la calidad de los suelos agrícolas provocando efectos negativos en la inocuidad de los alimentos cosechados. El comportamiento de los residuos de clorpirifos depende de las propiedades edáficas especialmente la materia orgánica. El objetivo de la investigación fue determinar el efecto de la aplicación de vermicompost en la residualidad y disipación de clorpirifos en un suelo entisol de clima semiárido con variaciones en su fertilidad edáfica. Se estudiaron cuatro tratamientos. T1: 7.5% vermicompost (V) y 92.5% suelo (S); T2: 5% V y 95% S; T3: 2.5% V y 97.5% S; T4: 0% V y 100% S. Los tratamientos fueron sometidos a un periodo de incubación de siete días. Posteriormente, se aplicó una dosis de clorpirifos de 1.5 mg kg-1 como ingrediente activo en una sola oportunidad por cada tratamiento. Se utilizó un diseño completamente al azar con tres repeticiones por cada tratamiento conformando 12 unidades experimentales. La determinación analítica de clorpirifos se efectuó por cromatografía líquida de alta resolución (HPLC). Las evaluaciones se realizaron a 1,7, 14, 28, 42, 70 y 98 días de la aplicación de clorpirifos. Los resultados demostraron que la incorporación de vermicompost al suelo en niveles crecientes promovió la disminución de la concentración residual de clorpirifos y facilitó su disipación de manera gradual. Las curvas de disipación de clorpirifos corresponden a una ecuación logarítmica de primer orden donde los efectos de los tratamientos ocasionaron mayor disipación de clorpirifos conforme transcurrió el periodo de evaluación. A 98 días de evaluación el T1 registró menor residualidad (0.786 m kg-1), mayor tasa de disipación diaria (0.008) y menor vida media de clorpirifos (86.64 días). Al final del periodo de investigación, los niveles edáficos de P, K, CE y CIC se incrementaron mientras que la MO, N y pH descendieron luego de la aplicación de vermicompost.

RESUMEN En la región Amazonas del Perú, los arándanos nativos se encuentran en zonas altoandinas, donde se asocian con pequeños arbustos y musgos. Estos arándanos prefieren suelos poco profundos con textura arcillo-arenosa y de baja fertilidad. Sin embargo, su población se ve amenazada por su limitada capacidad regenerativa y diversos factores antropogénicos. El objetivo de este estudio fue evaluar la influencia del ácido indol butírico y del tipo de sustrato en la propagación vegetativa del arándano nativo peruano (Vaccinium sp.) cultivado en microtúnel. El experimento se realizó bajo un diseño completo al azar con arreglo factorial; A: sustrato (arena 100%, arena + fibra de coco 1:1 v/v y fibra de coco 100%) y B: concentraciones de AIB (1, 2 y 3 g L-1) y un testigo (0 g L-1), dando 12 tratamientos, 3 repeticiones y 36 unidades experimentales. En el campo, se recolectaron brotes apicales de arándano con tallos semi-lignificados y vigorosos. En el laboratorio, fueron desinfectados con fungicida y cortados a un tamaño de 7 cm. Posteriormente, se trataron con AIB y se plantaron en sustratos preparados. Tras 120 días en el microtúnel, se observó que los esquejes de arándano mostraron una mayor supervivencia en arena + fibra de coco (94,7%), enraizamiento (73,8%), número de raíces (6,9) y crecimiento radicular (9,2). Las concentraciones de AIB a 2 y 3 g L-1 tuvieron un mayor efecto en el enraizamiento, con valores superiores al 60%. El uso de sustratos y concentraciones adecuadas de auxina favorece significativamente la propagación vegetativa del arándano nativo. Esta técnica de propagación se postula como una alternativa prometedora para la propagación masiva de esta especie con fines de conservación y rehabilitación de ecosistemas degradados.

ABSTRACT In the Amazon region of Peru, native blueberries are found in high Andean areas, where they are associated with small shrubs and mosses. These blueberries prefer shallow soils with a sandy-clay texture and low fertility. However, their population is threatened by their limited regenerative capacity and various anthropogenic factors. The aim of this study was to evaluate the influence of indole butyric acid and substrate type on the vegetative propagation of native Peruvian blueberry (Vaccinium sp.) grown in microtunnel. The experiment was conducted under a complete randomized design with the factorial arrangement; A: substrate (sand 100%, sand + coconut fiber 1:1 v/v and coconut fiber 100%) and B: AIB concentrations (1, 2 and 3 g L-1) and control (0 g L-1), giving 12 treatments, three replicates and 36 experimental plots. In the field, apical shoots of blueberry with semi-lignified and vigorous stems were collected. In the laboratory, they were disinfected with fungicide and cut to a size of 7 cm. Subsequently, they were treated with AIB and planted in prepared substrates. After 120 days in the microtunnel, it was observed that blueberry cuttings showed higher survival in sand + coconut fiber (94.7%), rooting (73.8%), root number (6.9), and root growth (9.2). AIB concentrations at 2 and 3 g L-1 had a greater effect on rooting, with values above 60%. The use of suitable substrates and auxin concentrations significantly favors the vegetative propagation of native blueberries. This propagation technique is postulated as a promising alternative for the mass propagation of this species for the conservation and rehabilitation of degraded ecosystems.

RESUMO Os sistemas de produção integrados surgiram como uma estratégia de produção sustentável na intensificação do uso da terra com impacto mínimo nas funções do ecossistema. Este estudo utilizou ferramentas de agricultura de precisão para investigar a variabilidade espacial de propriedades do solo e da vegetação em área de Integração Lavoura-Pecuária-Floresta (ILPF) e área de Integração Pecuária-Floresta (IPF) em São Carlos, SP, Brasil. Consequentemente, foram avaliados: (1) as propriedades físico-químicas do solo, (2) a resistividade elétrica, e (3) os atributos da cultura do Eucalyptus urograndis que compõem a área de estudo. As amostragens foram realizadas em (1) agosto de 2021 nas profundidades de 0-0,2 e 0,2-0,4 m; (2) outubro de 2021 nas profundidades de 0-0,5, 0-1,0 e 0-2,0 m; e (3) abril de 2021 e abril de 2022. Os resultados indicaram que os valores dos parâmetros de fertilidade do solo foram adequados ao desenvolvimento da cultura e apresentaram baixa variabilidade espacial. As análises de solo indicaram baixa acidez, altos teores de matéria orgânica, teores médios de fósforo, potássio e capacidade de troca catiônica e altos teores de cálcio, magnésio e saturação por base. Entretanto, foram observados locais com maior variabilidade espacial para cálcio, refletindo variabilidade na capacidade de troca catiônica e saturação por base. A granulometria e a resistividade elétrica do solo apresentaram baixa variabilidade espacial na camada superficial. Mesmo sem diferenças nas propriedades do solo, houve diferença de 15% no maior volume de madeira no sistema IPF em comparação ao ILPF. ecossistema LavouraPecuáriaFloresta Lavoura Pecuária Floresta ILPF (ILPF PecuáriaFloresta (IPF Carlos SP Brasil Consequentemente avaliados 1 (1 físicoquímicas físico químicas 2 (2 3 (3 202 00,2 002 0 0,2 0-0, 0,20,4 0204 0,4 4 0,2-0, m 00,5, 005 0,5, 5 0-0,5 01,0 010 1,0 0-1, 02,0 020 2,0 0-2, 2022 acidez orgânica fósforo cálcio base Entretanto superficial 15 ( 20 00 00, 02 0, 0-0 0,20, 04 0,2-0 00,5 05 0,5 01 01, 10 1, 0-1 02, 2, 0-2 0- 0,20 0,2-

ABSTRACT Integrated production systems have emerged as a sustainable production strategy in intensifying land use with minimal impact on ecosystem functions. This study used precision agriculture tools to investigate the spatial variability of soil and vegetation properties in an integrated crop-livestock-forestry (ICLF) and in an integrated livestock-forestry (ILF) area of São Carlos city, SP, Brazil. Consequently, the following were assessed: (1) the soil physicochemical properties, (2) the electrical resistivity, and (3) the attributes of the Eucalyptus urograndis crop comprising the study area. Samplings were performed in (1) August 2021 at depths of 0-0.2 and 0.2-0.4 m; (2) October 2021 at depths of 0-0.5, 0-1.0, and 0-2.0 m; and (3) April 2021 and April 2022. Results indicated that the values for soil fertility parameters were suitable for crop development and presented low spatial variability. Soil analyses indicated low acidity, high levels of organic matter, average cation exchange capacity, phosphorus and potassium contents, high base saturation, and calcium and magnesium levels. However, locations with greater spatial variability for calcium were observed, reflecting cation exchange capacity and base saturation variability. Soil granulometry and electrical resistivity had a low spatial variability at the surface layer. Even without differences in the soil properties, there was a difference of 15% in the larger wood volume in the ILF then ICLF system. functions croplivestockforestry livestock forestry (ICLF livestockforestry (ILF city SP Brazil Consequently assessed 1 (1 2 (2 3 (3 202 00.2 002 0 0.2 0-0. 0.20.4 0204 0.4 4 0.2-0. m 00.5, 005 0.5, 5 0-0.5 01.0, 010 1.0, 0-1.0 02.0 020 2.0 0-2. 2022 acidity matter contents However observed layer 15 system ( 20 00 00. 02 0. 0-0 0.20. 04 0.2-0 00.5 05 0.5 01 01.0 10 1.0 0-1. 02. 2. 0-2 0- 0.20 0.2- 01. 1. 0-1

Abstract The aim of the research is to perform an exploratory review showing studies evaluating the use of autologous platelet-rich plasma (PRP) as an additional treatment to hormonal treatment to increase endometrial thickness in patients with infertility related to endometrial thickness. We conducted a literature search since 2015 to 2024, including studies that evaluated women with a history of failed fertility treatments with conventional methods, additionally gestational losses and decreased endometrial thickness. In the literature search we used databases such as Medline, Scopus and Google Scholar that helped to extract the necessary papers for the exploration. Additionally, we used Boolean terms to search for information that was later used in the PRISMA statement to synthesize the results obtained. In the reviewed studies we found that hormonal treatment combined with autologous platelet-rich plasma application could increase endometrial thickness and positively impact fertility of treated woman. Finally, it is concluded that more quality studies and larger number of patients in which PRP is included as an additional treatment to hormonal therapy are needed to determine its impact on endometrial thickness.

Resumen Se realiza una revisión exploratoria que muestra estudios donde se evalúa el uso del plasma rico en plaquetas (PRP) autólogo como tratamiento adicional al tratamiento hormonal para aumentar el grosor del endometrio en pacientes con infertilidad relacionada con el grosor endometrial. Realizamos una búsqueda de la literatura desde el año 2015 hasta el 2024 incluyendo estudios que evaluaron mujeres con antecedentes de tratamientos de fertilidad fallidos con métodos convencionales, adicionalmente pérdidas gestacionales y disminución del grosor del endometrio. En la búsqueda de la literatura utilizamos bases de datos como Medline, Scopus y Google Scholar que ayudaron a extraer los trabajos necesarios para la exploración. Adicionalmente usamos términos booleanos para la búsqueda de información que posteriormente se utilizaron en la declaración PRISMA para sintetizar los resultados obtenidos. En los estudios revisados encontramos que el tratamiento hormonal combinado con la aplicación de PRP autólogo podría aumentar el grosor endometrial e impactar positivamente en la fertilidad de las pacientes tratadas. Finalmente, se concluye que son necesarios más estudios de calidad y mayor número de pacientes en los que se incluya el PRP como tratamiento adicional a la terapia hormonal para determinar su repercusión sobre el grosor endometrial.

Abstract Endometriosis is a chronic disease, characterized by the presence of endometrium outside the uterine cavity, which affects around 10% of women of reproductive age. Symptoms vary widely and can be absent or severe, debilitating and highly restrictive of quality of life. Pain, in different forms, intensities and associations, is the most striking symptom. The endometriosis is often associated with infertility and other comorbidities that represent additional risks for patients. Diagnosing is a major challenge. It is often delayed, for reasons rooted in the unspecific nosology of the disease, which is confused with common conditions. Another major challenge is treatment, since it is a chronic, multifaceted disease, sometimes with systemic repercussions, for which it is essential to find a personalized response. This review highlights the most recent aspects of pathogenesis and treatment options, particularly the new pharmacological options with GnRH antagonists, reserving surgery for specific situations. New molecular agents are being developed for the diagnosis and potential treatment of this disease. The configuration of clinical guidelines for optimizing the fertility potential of these patients remains to be defined.

Resumo A endometriose é uma doença crónica, caracterizada pela presença de endométrio fora da cavidade uterina, que afeta cerca de 10% das mulheres em idade reprodutiva. A sua expressão sintomática é muito variada, pode estar ausente ou ser grave, debilitante e altamente condicionante da qualidade de vida. A dor, com matizes e associações diversas, é o sintoma mais marcante. Associa-se com frequência a infertilidade e outras comorbilidades que representam riscos adicionais para as doentes. O diagnóstico da endometriose é um grande desafio. O atraso é frequente, por razões que radicam na nosologia pouco especifica da doença, que se confunde com situações comuns. Outro grande desafio é o tratamento, uma vez que é uma doença crónica, multifacetada, por vezes com repercussão sistémica, para a qual é indispensável encontrar uma resposta personalizada. Esta revisão destaca os aspetos mais recentes da patogénese e opções do tratamento, nomeadamente as novas opções farmacológicas com antagonistas da GnRH, reservando a cirurgia para situações específicas. Estão em desenvolvimento novos agentes moleculares para o diagnóstico e potencial tratamento desta doença. A configuração de orientações clínicas para a otimização do potencial de fertilidade dessas doentes continua por definir.

ABSTRACT Residues generated in sugarcane production such as biochar, originated from bagasse burning, are used as a fertilizer source, which can increase forage dry matter yield, as well as improving soil chemical attributes. The objective of this study was to evaluate the effects of using biochar from sugarcane bagasse burning and mineral fertilization, as well as their association, on the dry matter yield of Urochloa decumbens and on the chemical attributes of the soil. The design was randomized blocks, which consisted of eight treatments and four blocks. The treatments were: T1- mineral fertilization; T2- fertilization with limestone plus mineral fertilizer; T3- liming; T4- biochar; T5- biochar plus mineral fertilizer; T6- liming plus biochar; T7- double fertilization with biochar and T8- control. Association of mineral fertilizer and biochar was able to increase dry matter yield by up to 131% when compared to the control treatment during the first year. The use of fertilization strategies in the restoration of degraded area under U. decumbens does not interfere with soil chemical attributes, except for sulfur. source association blocks were T1 T T2 T3 T4 T5 T6 T7 T8 131 year U sulfur 13 1

RESUMO Resíduos gerados pela produção de cana-de-açúcar como o biochar, proveniente da queima do bagaço podem ser utilizados como fonte de fertilizante, podendo incrementar a produção de matéria seca de forrageira, bem como, dos atributos químicos do solo. O objetivo deste trabalho foi avaliar a utilização de biochar da queima do bagaço da cana-de-açúcar e da adubação mineral e associação de ambos, na produtividade de matéria seca de Urochloa decumbens, e nos atributos químicos do solo. O delineamento utilizado foi em blocos casualizados, composto por oito tratamentos, com quatro repetições. Os tratamentos foram: T1- adubação mineral; T2- adubação com calcário + fertilizante mineral; T3- calagem; T4- biochar; T5- biochar + adubação mineral; T6- calagem + biochar; T7- duas vezes a dose com biochar e T8- controle. A associação de fertilizante mineral com biochar foi capaz de aumentar a produção de matéria seca em até 131% quando comparado ao tratamento controle no primeiro ano. O emprego de diferentes estratégias de fertilização na recuperação de área degradada sob uso de Urochloa decumbens, não interfere nos atributos químicos do solo, com exceção do elemento enxofre. canadeaçúcar cana açúcar forrageira solo ambos decumbens casualizados repetições foram T1 T T2 T3 T4 T5 T6 T7 T8 131 ano enxofre 13 1

RESUMEN: La epilepsia es una enfermedad crónica no transmisible que afecta al sistema nervioso más prevalente en el mundo. Dentro de los tratamientos, uno de los fármacos más utilizados es el ácido valproico (AVP), el que ocasiona diversos efectos secundarios. Entre los órganos que se pueden ver afectados se encuentra la gónada masculina, en donde se ha visto que hombres en tratamiento con AVP reducen sus tasas de fecundidad, además de causar trastornos endocrinos disminuyendo andrógenos y gonadotrofinas. En modelos animales, se ha visto que disminuye los pesos de las glándulas anexas al tracto reproductor masculino, como también a nivel testicular, disminuyendo la concentración espermática y aumentando el recuento de células apoptóticas. Estos efectos se deberían a que el AVP aumenta las especies reactivas de oxígeno (ROS), ocasionando daño en macromoléculas, afectando todos los procesos celulares sensibles a óxido reducción. A lo largo del desarrollo testicular, in utero se ha visto que la expresión de enzimas antioxidantes como superóxido dismutasa, catalasa y glutatión peroxidasa, son más bajos durante el desarrollo embrionario temprano, como también la vitamina E (VE) se encuentra disminuida. Por tanto, no resultan suficientes para revertir los efectos tóxicos de las ROS. El objetivo de esta revisión fue asociar el uso de AVP durante la gestación y sus efectos a nivel del desarrollo testicular y describir el potencial rol protector de la VE.

ABSTRACT: Epilepsy is the chronic non-communicable disease of the nervous system most prevalent in the world. Valproic acid (VPA) is one of the most used drugs in the treatment of epilepsy but with various side effects. One of the organs that can be affected is the testis, where it has been seen that men treated with VPA reduce their fertility rates, in addition to causing endocrine disorders by decreasing androgens and gonadotropins. In animal models, it has been shown to reduce the weights of the glands attached to the male reproductive tract, as well as at the testicular level, decreasing sperm concentration and increasing apoptotic cell count. These effects are because VPA increases reactive oxygen species (ROS), causing damage to macromolecules and affecting all cellular processes sensitive to oxide reduction. Throughout testicular development, in utero, it has been seen that the expression of antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase, are lower during early embryonic development, as well as vitamin E (VE) is decreased. Therefore, they are not sufficient to reverse the toxic effects of ROS. The objective of this study was to review the use of VPA during pregnancy, its effect on testicular development, and to explore the potential protective role of vitamin E.

ABSTRACT Introduction Obstructive azoospermia occurs when there is a blockage in the male reproductive tract, leading to a complete absence of sperm in the ejaculate. It constitutes around 40% of all cases of azoospermia (1, 2). Blockages in the male reproductive tract can arise from either congenital or acquired factors, affecting various segments such as the epididymis, vas deferens, and ejaculatory ducts (3). Examples of congenital causes encompass conditions like congenital bilateral absence of the vas deferens and unexplained epididymal blockages (4). Acquired instances of obstructive azoospermia may result from factors like vasectomy, infections, trauma, or unintentional injuries caused by medical procedures (5). This complex condition affecting male fertility, presents two main treatment options: microsurgical reconstruction and surgical extraction of sperm followed by in vitro fertilization (IVF). Microsurgical reconstruction proves to be the most cost-effective option for treating obstructive azoospermia when compared with assisted reproductive techniques (6, 7). However, success rates of reconstruction defined by patency are as high as 99% for vasovasostomy (VV) but decline to around 65% if vasoepididymostomy (VE) is required (8, 9). Thus, continued refinement in technique is necessary in order to attempt to improve patency for patients undergoing VE. In this video, we show a comprehensive demonstration of microsurgical VE, highlighting the innovative epididymal occlusion stitch. The goal of this innovative surgical technique is to improve outcomes for VE. Materials and Methods The patient is a 39-year-old male diagnosed with obstructive azoospermia who presents for surgical reconstruction via VE. His partner is a 37-years-old female with regular menstrual cycles. The comprehensive clinical data encompasses a range of factors, including FSH levels, results from semen analysis, and outcomes from testicular sperm aspiration. This thorough exploration aims to provide a thorough understanding of our innovative surgical technique and its application in addressing complex cases of obstructive azoospermia. Results The procedure was started on the right, the vas deferens was identified and transected. The abdominal side of the vas was intubated and a vasogram performed, there was no obstruction. There was no fluid visible from the testicular side of the vas for analysis, thus we proceeded with VE. Upon inspection of the epididymis dilated tubules were identified. After selecting a tubule for VE, two 10-0 nylon sutures were placed, and it was incised. Upon inspection of the fluid motile sperm was identified. After VE, we performed a novel epididymal occlusion stitch technique. This was completed distal to the anastomosis by placing a 7-0 prolene through the tunica of the epididymis from the medial to lateral side. This stitch was then tightened down with the goal to largely occlude the epididymis so that sperm will preferentially travel through the anastomosis. The steps were then repeated on the left. At 3-month follow up, the patient had no change in testicular size as compared with preoperative size (18cc), he had no testicular or incisional discomfort, and on semen analysis he had presence of motile sperm. After 3 months post-surgery, the patient had motile sperm seen on semen analysis. Discussion The introduction of a novel epididymal occlusion stitch demonstrates a targeted strategy to enhance the success of microscopic VE. Encouragingly, a 3-month post-surgery follow-up reveals the presence of motile sperm, reinforcing the potential efficacy of our approach. This is promising given the historical lower patency, delayed time to patency, and higher delayed failure rates that patients who require VE experience (10). In total, 40% of all azoospermia cases can be attributed to obstruction. The conventional treatments for obstructive azoospermia involve microsurgical reconstruction and surgical sperm retrieval followed by IVF. While microsurgical reconstruction has proven to be economically viable, the quest for enhanced success rates has led to the exploration of innovative techniques. Historically, the evolution of VV and VE procedures, initially performed in the early 20th century, laid the foundation for contemporary microsurgical approaches (11). Notably, the microscopic VV demonstrated significant improvements in patency rates and natural pregnancy likelihood, as evidenced by the seminal Vasovastomy Study Group study in 1991 (8). In contemporary literature, success rates particularly for VE remain unchanged for the past three decades since the original published success rates by the Vasectomy Reversal Study Group (12). VE is associated with a longer time to patency as well with patients taking 2.8 to 6.6 months to have sperm return to ejaculate as compared to 1.7 to 4.3 months for those undergoing VV. Additionally, of those patients who successfully have sperm return to the ejaculate after VE up to 50% will have delayed failure compared to 12% for those undergoing VV who are patent. Finally, of those who experience delayed failure after undergoing VE it usually occurs earlier with studies reporting as early as 6 months post-operatively (20). Given the lack of improvement and significantly worsened outcomes with VE further surgical refinement is a constant goal for surgeons performing this procedure. Conclusion: In conclusion, this video is both a demonstration and a call to action for commitment to surgical innovation. We aim to raise the bar in VE success rates, ultimately bringing tangible benefits to patients and contributing to the ongoing evolution of reproductive medicine. The novel epididymal occlusion stitch emerges as a beacon of progress, promising not only enhanced safety but also potential reductions in patency time. Surgical excellence and methodological refinement, as exemplified in this video, lay the foundation for a future where male reproductive surgery continues to break new ground. 40 1, 1 (1 2. 2 . 2) 3. (3) 4. 4 (4) vasectomy infections trauma 5. 5 (5) fertility options IVF (IVF) costeffective cost effective 6, (6 7. 7 7) However 99 (VV 65 (VE 8, 8 (8 9. 9 9) Thus 39yearold yearold 39 year old 37yearsold yearsold 37 years cycles levels aspiration right transected obstruction 100 10 0 10- placed incised 70 7- left 3month month 18cc, 18cc cc , (18cc) discomfort postsurgery, postsurgery post surgery, Encouragingly followup approach 10. (10) total viable Historically th century 11. 11 (11) Notably likelihood 199 8. (8) literature 12. 12 (12) 28 66 6. 17 1. 43 Additionally 50 patent Finally postoperatively operatively 20. 20 (20) Conclusion conclusion innovation medicine progress ground ( (3 (4 (5 (IVF (18cc (10 (11 19 (12 (20 (2

ABSTRACT Purpose Varicocele is a condition known to cause damage to seminal parameters and sperm function. Furthermore, it has been hypothesized that the varicocele effect on fertility is time-dependent; however, little is known about the consequences of its establishment time on reproductive organs and/or sperm function. This study aimed to evaluate the effect of the duration of experimental varicocele on reproductive organs, sperm parameters, and sperm function. Materials and Methods Varicocele induction surgeries were performed in Wistar rats aged 40 or 100 days old. At 160-day-old, analyses were performed, including biometry of reproductive organs (prostate, seminal vesicles, epididymis, and testis), sperm parameters (vitality, morphology, and motility), and sperm function tests (nuclear DNA integrity, acrosome integrity, and mitochondrial activity). Results The analysis of the biometry of reproductive organs showed no differences between distinct ages in which varicocele was induced. The total abnormal sperm morphology was bigger in animals with varicocele induced to 100 days old than in animals with varicocele induced to 40 days old. Regarding nuclear DNA integrity, animals of varicocele induced to 100 days old showed worse results compared to animals of varicocele induced to 40 days old. Other parameters analyzed showed no differences between varicocele groups. Conclusion In this study conducted on rats, we conclude that varicocele adversely affects sperm, particularly its function. However, we did not observe a negative progressive effect on sperm. Furthermore timedependent dependent time-dependent however andor 4 10 160dayold, 160dayold dayold 160 day old, 160-day-old prostate, prostate (prostate vesicles epididymis testis, testis , testis) vitality, vitality (vitality motility, motility motility) integrity activity. activity . activity) groups However 1 16

ABSTRACT The cowpea is a legume with a high nutritional value and represents one of the most important self-consumption items in the country. However, under storage conditions its main problem are bruchids, which decrease the nutritional value and fertility of the seeds. Bruchids spread more frequently unintentionally throughout the world, this is due their adaptability. In the first months of 2023, damage was observed to stored cowpea grains of the species Vigna unguiculata, presenting round holes, decreasing the nutritional value and fertility of the seeds. The inspection of grains recorded the presence of a beetle, which was identified as Decellebruchus atrolineatus, Family Chrysomelidae, Subfamily Bruchinae.

RESUMEN El poroto es una leguminosa comestible con un alto valor nutritivo y representa uno de los rubros de autoconsumo más importantes en el país. Sin embargo, en condiciones de almacenamiento su principal problema son los brúquinos, quienes disminuyen el valor nutricional y la fertilidad de las semillas. Por la adaptación que poseen, los brúquinos, se diseminan con mayor frecuencia de manera involuntaria. En los primeros meses del año 2023, fueron observados daños en granos almacenados de poroto de la especie Vigna unguiculata, presentando orificios redondeados. La inspección de dichos granos registró la presencia de un coleóptero, que fue identificado como Decellebruchus atrolineatus, Familia Chrysomelidae, Subfamilia Bruchinae.

Abstract Objective: Ovarian torsion (OT) represents a severe gynecological emergency in female pediatric patients, necessitating immediate surgical intervention to prevent ovarian ischemia and preserve fertility. Prompt diagnosis is, therefore, paramount. This retrospective study set out to assess the utility of combined clinical, ultrasound, and laboratory features in diagnosing OT. Methods: The authors included 326 female pediatric patients aged under 14 years who underwent surgical confirmation of OT over a five-year period. Logistic regression analysis was employed to pinpoint factors linked with OT, and the authors compared clinical presentation, laboratory results, and ultrasound characteristics between patients with OT (OT group) and without OT (N-OT group). The authors conducted receiver operating characteristic (ROC) curve analysis to gauge the predictive capacity of the combined features. Results: Among 326, OTwas confirmed in 24.23 % (79 cases) of the patients. The OT group had a higher incidence of prenatal ovarian masses than the N-OT (22 cases versus 7 cases) (p < 0.0001). Similarly, the authors observed significant differences in the presence of lower abdominal pain, suspected torsion on transabdominal ultrasound, and a high neutrophil-lymphocyte ratio (NLR > 3) between the OTand non-OT groups (p < 0.05). Furthermore, when these parameters were combined, the resulting area under the curve (AUC) was 0.868, demonstrating their potential utility in OT diagnosis. Conclusion: This study demonstrates a prediction model integrating clinical, laboratory, and ultrasound findings that can support the preoperative diagnosis of ovarian torsion, thereby enhancing diagnostic precision and improving patient management. Future prospective studies should concentrate on developing clinical predictive models for OTin pediatric patients. Objective fertility is therefore paramount Methods 32 1 fiveyear five year period presentation results NOT N group. . ROC (ROC Results 2423 24 23 24.2 79 (7 22 (2 p 0.0001. 00001 0.0001 0 0001 0.0001) Similarly pain neutrophillymphocyte neutrophil lymphocyte NLR 3 nonOT non 0.05. 005 0.05 05 0.05) Furthermore AUC (AUC 0868 868 0.868 Conclusion management 242 2 24. ( 0000 0.000 000 00 0.0 086 86 0.86 0.00 0. 08 8 0.8