ABSTRACT The present study aimed to evaluate the ecology considering distribution, abundance, population structure, and fecundity of the marine diplostraceans Penilia avirostris and Pseudevadne tergestina in the Veracruz Reef System National Park, southwest Gulf of Mexico. Surface samples were collected with a 300 μm mesh plankton net at 26 sampling sites in June, August, October (2011), May (2012) and June (2013). Temperature, salinity, and dissolved oxygen were measured in situ. The temperature range was 26.53 ± 0.41 and 29.29 ± 0.27°C, salinity between 33.43 ± 0.49 and 35.91 ± 0.09, and dissolved oxygen between 2.92 ± 0.08 and 6.56 ± 0.08 mg L-1. P. avirostris and P. tergestina presented the highest density, with 2200 ind 100 m-3 in June and 5980 ind 100 m-3 in October, respectively. Four stages were determined in P. avirostris. In P. tergestina, there were five instars, with males in August and October. The size of parthenogenic females of P. avirostris was 773.4 ± 20.08 μm. In P. tergestina, it was 637.6 ± 49.28 μm. The number of embryos in P. avirostris was 2 to 7, and in P. tergestina, 2 to 8 embryos. The correlations between body length and the number of embryos in both species were statistically significant under the potential model (r > 0.68 for both species, P < 0.001). The transects with the highest abundance were the northern and the southern zones because they are far from the influence of the Jamapa River. The density of these crustaceans is related to optimal temperature, salinity, and dissolved oxygen values. The low proportions obtained from gamogenic individuals of P. avirostris and P. tergestina suggest that in tropical and subtropical regions, gamogenesis is not crucial for the life cycle of these organisms.

Abstract Nototheniidae is the most numerous family of notothenioids and, although they are mainly Antarctic, they are also well represented in Patagonian waters. In both Antarctic and sub-Antarctic waters, they adopt a wide variety of reproductive strategies. In Antarctic waters they usually have a biennial reproductive cycle, whereas in sub-Antarctic ones, they reproduce annually or biannually. In the intertidal zone of central Patagonia the most abundant nototheniid fish is Patagonotothen cornucola and, despite this, there is little information about their biology. The aim of this work was to study its reproductive cycle in a rocky intertidal coast of Argentine Patagonia by means of histological and morphological analyses. Samples were collected monthly at two intertidal sites in the San Jorge Gulf. Condition factor, gonadosomatic and hepatosomatic indexes were calculated and thin sections of gonads were stained with one-step trichrome to determine gonadal stages. Relative and potential fecundity were also estimated. Males and females mature synchronously and reach maximum gonadosomatic index between July and August. In females the hepatosomatic index increases synchronously with gonadosomatic index, while in males it remains constant throughout the cycle. It is concluded that P. cornucola has an annual reproductive cycle and that spawning occurs in winter. Maximum potential fecundity is approximately 27,095 oocytes in a 11.7 cm female and it increases with size.

Resumen La familia Nototheniidae es la más numerosa entre los nototénidos y, a pesar de ser principalmente Antárticos, están muy bien representados en aguas patagónicas. Desde la Antártida hasta las aguas de Sudamérica, han adoptado una gran variedad de estrategias reproductivas. Los peces antárticos suelen tener un ciclo reproductivo bienal mientras que en aguas sub-antárticas los nototénidos pueden reproducirse anualmente o bianualmente. En la zona intermareal de la Patagonia central, el pez más abundante es Patagonotothen cornucola y, a pesar de esto, hay poca información sobre su biología. Por esto, el objetivo principal de este trabajo fue estudiar su ciclo reproductivo en la zona intermareal rocosa usando métodos histológicos y morfológicos. Las muestras fueron recolectadas mensualmente en dos sitios intermareales en el Golfo San Jorge, y se calculó el factor de condición, índices hepatosomáticos y gonadosomáticos y se realizaron cortes histológicos de las gónadas que fueron teñidas con tricrómico de un tiempo para determinar los estadios gonadales, además se estimó la fecundidad potencial y relativa. Se determinó que machos y hembras maduran sincrónicamente alcanzando los valores máximos de índices gonadosomáticos entre julio y agosto. En las hembras el índice hepatosomático aumenta simultáneamente con el gonadosomático mientras que en machos se mantiene constante durante todo el ciclo. Se concluye que P. cornucola tiene un ciclo reproductivo anual y que el desove ocurre en el invierno. La fecundidad potencial máxima fue de 27.095 oocitos en una hembra de 11,7 cm y se incrementa con la talla.

RESUMEN Fundamento el estudio de la fecundidad adolescente, así como su cambio en el tiempo es de gran importancia, ya que permite mostrar los avances o retrocesos de la misma. Objetivo caracterizar el comportamiento de la fecundidad adolescente en la provincia de Cienfuegos. Métodos estudio descriptivo, de corte transversal, que tuvo como universo de investigación toda la población femenina de 15-49 años de Cienfuegos en el período 2012-2021, así como los nacimientos ocurridos por este grupo de edad, además los de las madres menores de 15 años. Se analizaron los indicadores: tasa específica de fecundidad, haciendo énfasis en la tasa de fecundidad adolescente (15-19 años), edad media y peso de la tasa de fecundidad adolescente al total de la fecundidad. Los datos fueron obtenidos de los anuarios estadísticos de la Oficina Nacional de Estadística e Información, de la Dirección Nacional y Provincial de Estadísticas del Ministerio de Salud Pública. Resultados la tasa de fecundidad adolescente presenta ligeras oscilaciones, pasando de 46,7 hijos por mujer en el año 2012 a 45, 4 en el 2021. El grupo de edad de mayor fecundidad es el 20-24 años, la edad media se ubica en el grupo etario 25-29 años y los municipios de mayor peso a la fecundidad total son Abreus y Aguada de Pasajeros. Conclusiones la tasa de fecundidad adolescente muestra resistencia a la disminución, comportamiento que demuestra la necesidad de enfatizar en las políticas y estrategias de educación sexual a los grupos de edades más vulnerables dentro del grupo reproductivo.

ABSTRACT Foundation: the adolescent fertility study, as well as its change over time, is of great importance, since it allows us to show its progress or setbacks. Objective: characterize the fertility behavior in the Cienfuegos province. Methods: descriptive, cross-sectional study, which had as the research universe the entire female population aged 15-49 years old of Cienfuegos from 2012 to 2021, as well as the births that occurred in this age group, in addition to those of 15 years old younger mothers. The analyzed indicators were: specific fertility rate, emphasizing the adolescent fertility rate (15-19 years old), average age and weight of the adolescent fertility rate to total fertility. The data were obtained from the National Office of Statistics and Information statistical yearbooks, of the National and Provincial Public Health Ministry Directorate of Statistics. Results: the adolescent fertility rate presents slight oscillations, going from 46.7 children per woman in 2012 to 45.4 in 2021. The age group with the highest fertility is 20-24 years old, the average age is in the age group 25-29 years old and the municipalities with the greatest weight in total fertility are Abreus and Aguada de Pasajeros. Conclusions: the adolescent fertility rate shows resistance to decline, a behavior that demonstrates the need to emphasize sexual education policies and strategies for the most vulnerable age groups within the reproductive group.

Abstract A total of 381 specimens of the tilapia Oreochromis mossambicus collected monthly from May 2017 to May 2018 in the Laguna de Los Patos, Cumaná, Venezuela, to evaluate reproductive parameters of this non-native species. Significant differences were found in relation to average height and weight between males and females, with the highest values in males. The sex ratio was 1:1.5 (males:females), which deviates significantly from the expected 1:1 ratio. The mean length of sexual maturity (Lm50) was 18.0 cm in females and 20.1 cm in males, reflecting that females mature at smaller sizes than males. The monthly variations of the gonadosomatic index (GSI) and the stages of sexual maturity show two reproductive peaks during the study, in October 2017 and April 2018, coinciding with the rainy and dry seasons in the region respectively. The condition factor (CF) showed significant differences between months, but not between sexes, with an average of 1.87 in females and 1.84 in males. The average absolute fecundity was 921 ± 604.6 eggs per fish, with a relative fecundity of 8.36 ± 3.09 eggs per gram of fish. Differences in oocyte size in mature females confirm that the species can spawn repeatedly over a period, which is considered an important factor for the establishment of tilapia in non-native environments. 38 201 Patos Cumaná Venezuela nonnative non native 11.5 115 1 1.5 5 1:1. malesfemales, malesfemales , (males:females) 11 1: Lm50 Lm (Lm50 180 18 0 18. 20 20. GSI (GSI study respectively CF (CF months sexes 187 87 1.8 184 84 92 6046 604 6 604. fish 836 8 36 8.3 309 3 09 3.0 period environments 11. 15 1. (males:females Lm5 (Lm5 2 9 60 83 8. 30 3. (Lm

Resumo Um total de 381 espécimes da tilápia Oreochromis mossambicus foram coletados mensalmente de maio de 2017 a maio de 2018, na Laguna de Los Patos, Cumaná, Venezuela, para avaliar parâmetros reprodutivos desta espécie não nativa. Foram encontradas diferenças significativas em relação às médias de altura e peso entre machos e fêmeas, sendo os maiores valores no sexo masculino. A razão sexual foi de 1:1,5 (machos:fêmeas), o que se desvia significativamente da proporção esperada de 1:1. O comprimento médio de maturação sexual (Lm50) foi de 18,0 cm nas fêmeas e 20,1 cm nos machos, refletindo que as fêmeas amadurecem em tamanhos menores que os machos. As variações mensais do índice gonadossomático (IGS) e dos estágios de maturidade sexual mostram dois picos reprodutivos durante o estudo, em outubro de 2017 e abril de 2018, coincidindo com as estações chuvosa e seca na região, respectivamente. O fator de condição (FC) apresentou diferenças significativas entre os meses, mas não entre os sexos, com média de 1,87 no sexo feminino e 1,84 no masculino. A fecundidade absoluta média foi de 921 ± 604,6 ovos por peixe, com fecundidade relativa de 8,36 ± 3,09 ovos por grama de peixe. Diferenças no tamanho dos ovócitos em fêmeas maduras confirmam que a espécie pode desovar repetidamente ao longo de um período, o que é considerado um fator importante para o estabelecimento de tilápias em ambientes não nativos. 38 201 2018 Patos Cumaná Venezuela nativa masculino 11,5 115 1 1,5 5 1:1, machosfêmeas, machosfêmeas , (machos:fêmeas) 11. 11 1. 1:1 Lm50 Lm (Lm50 180 18 0 18, 20 20, IGS (IGS estudo região respectivamente FC (FC meses sexos 187 87 1,8 184 84 92 6046 604 6 604, peixe 836 8 36 8,3 309 3 09 3,0 período nativos 11, 15 1, (machos:fêmeas 1: Lm5 (Lm5 2 9 60 83 8, 30 3, (Lm

Resumo O ácaro da tamareira, Oligonychus afrasiaticus (McGregor) (Acari: Tetranychidae), é uma praga grave das tâmaras no Médio Oriente e Norte de África, causando graves danos econômicos se não for controlado precocemente. Como os ácaros predadores são conhecidos por serem potenciais agentes de biocontrole contra diversas pragas, também a capacidade de predação, tabela de vida, reprodução e sobrevivência de Amblyseius swirskii Athias-Henriot e Neoseiulus cucumeris (Oudemans) (Acari: Phytoseiidae), coletados em fazendas de tamareiras em Qassim Arábia Saudita, foram estudados em condições de laboratório (25 °C, 30 °C, 35 °C e 50 ± 5% UR) contra todos os estágios móveis de O. afrasiaticus. Para ambos os predadores, o tempo médio de desenvolvimento, o período de oviposição e a longevidade foram inversamente relacionados à temperatura de 25 °C a 35 °C. Vários parâmetros foram estudados para A. swirskii e N. cucumeris a 25 °C, 30 °C e 35 °C, ou seja, o tempo de desenvolvimento feminino, 9,37, 7,29, 5,56 e 10,67, 8,38, 6,45 d; período de oviposição, 19,77, 16,18, 13,94 e 15,90, 13,84, 10,64 d; longevidade, 29,39, 24,79, 20,64 e 25,42, 21,94, 17,39 dias; fecundidade, 31,91, 37,10, 42,16 e 21,75, 26,84, 30,56 ovos por fêmea, respectivamente. A taxa máxima de predação diária para ambos os predadores foi registrada a 35 °C durante o período de oviposição. A predação total de fêmeas de A. swirskii e N. cucumeris foi de 370,86, 387,54, 405,83, 232,14, 263,32, 248,85 presas a 25 °C, 30 °C e 35 °C respectivamente. A taxa máxima de reprodução de A. swirskii e N. cucumeris (3,02; 2,87 ovos/♀/dia) foi registrada a 35 °C, enquanto a mínima (2,00; 1,36 ovos/♀/dia) foi registrada a 25 °C. Os parâmetros da tabela de vida foram estimados como taxa reprodutiva líquida (Ro) 21,68, 25,94, 29,52 e 18,95, 20,25, 22,78; o tempo médio de geração (T) 24,92, 21,82, 18,24 e 26,30, 23,60, 20,56 d; a taxa intrínseca de aumento (rm) 0,181, 0,232, 0,248 e 0,170, 0,185, 0,196; a taxa finita de aumento (λ) 1,365, 1,551, 1,706 e 1,126, 1,324, 1,428 para A. swirskii e N. cucumeris a 25 °C, 30 °C e 35 °C respectivamente. Os resultados deste estudo sugerem que as duas espécies de fitoseídeos são promissores agentes de controle biológico de O. afrasiaticus em uma ampla faixa de temperaturas. tamareira McGregor (McGregor Acari (Acari Tetranychidae, Tetranychidae , Tetranychidae) África precocemente pragas AthiasHenriot Athias Henriot Oudemans (Oudemans Phytoseiidae, Phytoseiidae Phytoseiidae) Saudita (2 C 3 5 UR 2 N seja feminino 937 9 37 9,37 729 7 29 7,29 556 56 5,5 1067 10 67 10,67 838 8 38 8,38 645 6 45 6,4 d 1977 19 77 19,77 1618 16 18 16,18 1394 13 94 13,9 1590 15 90 15,90 1384 84 13,84 1064 64 10,6 2939 39 29,39 2479 24 79 24,79 2064 20 20,6 2542 42 25,42 2194 21 21,94 1739 17 17,3 dias fecundidade 3191 31 91 31,91 3710 37,10 4216 42,1 2175 75 21,75 2684 26 26,84 3056 30,5 fêmea respectivamente 37086 370 86 370,86 38754 387 54 387,54 40583 405 83 405,83 23214 232 14 232,14 26332 263 32 263,32 24885 248 85 248,8 3,02 302 02 (3,02 287 87 2,8 ovos/♀/dia ovosdia dia 2,00 200 00 (2,00 136 1 36 1,3 Ro (Ro 2168 68 21,68 2594 25,94 2952 52 29,5 1895 95 18,95 2025 20,25 22,78 2278 22 78 T (T 2492 92 24,92 2182 82 21,82 1824 18,2 2630 26,30 2360 23 60 23,60 2056 20,5 rm (rm 0181 0 181 0,181 0232 0,232 0248 0,24 0170 170 0,170 0185 185 0,185 0,196 0196 196 λ (λ 1365 365 1,365 1551 551 1,551 1706 706 1,70 1126 126 1,126 1324 324 1,324 1428 428 1,42 temperaturas ( 93 9,3 72 7,2 55 5, 106 8,3 4 6, 197 19,7 161 16,1 139 13, 159 15,9 138 13,8 10, 293 29,3 247 24,7 206 20, 254 25,4 219 21,9 173 17, 319 31,9 371 37,1 421 42, 217 21,7 268 26,8 305 30, 3708 370,8 3875 387,5 4058 40 405,8 2321 232,1 2633 263,3 2488 248, 3,0 (3,0 28 2, 2,0 (2,0 1, 216 21,6 259 25,9 295 29, 189 18,9 202 20,2 22,7 227 249 24,9 218 21,8 182 18, 26,3 236 23,6 205 018 0,18 023 0,23 024 0,2 017 0,17 0,19 019 155 1,55 70 1,7 112 12 1,12 132 1,32 142 1,4 9, 7, 8, 19, 16, 15, 24, 25, 21, 31, 37, 26, 370, 387, 405, 232, 263, 3, (3, (2, 22, 23, 01 0,1 0, 1,5 11 1,1 (3

Abstract The date palm mite, Oligonychus afrasiaticus (McGregor) (Acari: Tetranychidae), is a serious pest of dates in the Middle East and North Africa, inflicting severe economic damage if not controlled early. As predaceous mites are known to be potential biocontrol agents against several pests, so predation capacity, life table, reproduction, and survival of Amblyseius swirskii Athias-Henriot and Neoseiulus cucumeris (Oudemans) (Acari: Phytoseiidae), collected from date palm farms in Qassim Saudi Arabia, were studied under laboratory conditions (25 °C, 30 °C, 35 °C and 50 ± 5% RH) against all motile stages of O. afrasiaticus. For both predators, mean developmental time, oviposition period, and longevity were inversely related to temperature from 25 to 35 °C. Various parameters were studied for A. swirskii and N. cucumeris at 25 °C, 30 °C and 35 °C, i.e. the female developmental time, 9.37, 7.29, 5.56, and 10.67, 8.38, 6.45 d; oviposition period, 19.77, 16.18, 13.94 and 15.90, 13.84, 10.64 d; longevity, 29.39, 24.79, 20.64 and 25.42, 21.94, 17.39 d; fecundity, 31.91, 37.10, 42.16 and 21.75, 26.84, 30.56 eggs per female, respectively. The maximum daily predation rate for both the predators was recorded at 35 °C during the oviposition period. The total predation of A. swirskii and N. cucumeris female was 370.86, 387.54, 405.83, 232.14, 263.32, 248.85 preys at 25 °C, 30 °C and 35 °C respectively. The maximum reproduction rate of A. swirskii and N. cucumeris (3.02, 2.87 eggs/♀/day) was recorded at 35 °C while the minimum (2.00, 1.36 eggs/♀/day) was recorded at 25 °C. The life table parameters were estimated as net reproductive rate (Ro) 21.68, 25.94, 29.52 and 18.95, 20.25, 22.78; the mean generation time (T) 24.92, 21.82, 18.24 and 26.30, 23.60, 20.56 d; the intrinsic rate of increase (rm) 0.181, 0.232, 0.248 and 0.170, 0.185, 0.196; the finite rate of increase (λ) 1.365, 1.551, 1.706 and 1.126, 1.324, 1.428 for A. swirskii and N. cucumeris at 25 °C, 30 °C and 35 °C respectively. The results of this study suggested that the two phytoseiid species are promising biological control agents of O. afrasiaticus at a wide range of temperatures. mite McGregor (McGregor Acari (Acari Tetranychidae, Tetranychidae , Tetranychidae) Africa early pests capacity AthiasHenriot Athias Henriot Oudemans (Oudemans Phytoseiidae, Phytoseiidae Phytoseiidae) Arabia (2 C 3 5 RH O period 2 A N ie i e i.e 937 9 37 9.37 729 7 29 7.29 556 56 5.56 1067 10 67 10.67 838 8 38 8.38 645 6 45 6.4 d 1977 19 77 19.77 1618 16 18 16.18 1394 13 94 13.9 1590 15 90 15.90 1384 84 13.84 1064 64 10.6 2939 39 29.39 2479 24 79 24.79 2064 20 20.6 2542 42 25.42 2194 21 21.94 1739 17 17.3 fecundity 3191 31 91 31.91 3710 37.10 4216 42.1 2175 75 21.75 2684 26 26.84 3056 30.5 respectively 37086 370 86 370.86 38754 387 54 387.54 40583 405 83 405.83 23214 232 14 232.14 26332 263 32 263.32 24885 248 85 248.8 3.02, 302 02 (3.02 287 87 2.8 eggs/♀/day eggsday day 2.00, 200 00 (2.00 136 1 36 1.3 Ro (Ro 2168 68 21.68 2594 25.94 2952 52 29.5 1895 95 18.95 2025 20.25 22.78 2278 22 78 T (T 2492 92 24.92 2182 82 21.82 1824 18.2 2630 26.30 2360 23 60 23.60 2056 20.5 rm (rm 0181 0 181 0.181 0232 0.232 0248 0.24 0170 170 0.170 0185 185 0.185 0.196 0196 196 λ (λ 1365 365 1.365 1551 551 1.551 1706 706 1.70 1126 126 1.126 1324 324 1.324 1428 428 1.42 temperatures ( 93 9.3 72 7.2 55 5.5 106 8.3 4 6. 197 19.7 161 16.1 139 13. 159 15.9 138 13.8 10. 293 29.3 247 24.7 206 20. 254 25.4 219 21.9 173 17. 319 31.9 371 37.1 421 42. 217 21.7 268 26.8 305 30. 3708 370.8 3875 387.5 4058 40 405.8 2321 232.1 2633 263.3 2488 248. 3.02 (3.0 28 2. 2.00 (2.0 1. 216 21.6 259 25.9 295 29. 189 18.9 202 20.2 22.7 227 249 24.9 218 21.8 182 18. 26.3 236 23.6 205 018 0.18 023 0.23 024 0.2 017 0.17 0.19 019 155 1.55 70 1.7 112 12 1.12 132 1.32 142 1.4 9. 7. 5. 8. 19. 16. 15. 24. 25. 21. 31. 37. 26. 370. 387. 405. 232. 263. 3.0 (3. 2.0 (2. 22. 23. 01 0.1 0. 1.5 11 1.1 3. (3

ABSTRACT This study aimed to assess population and reproductive aspects of the ampithoid amphipod Cymadusa filosa Savigny, 1816 in a region with pollution by petrogenic hydrocarbons. Sampling of Sargassum furcatum Kützing, 1843 fronds and the associated fauna was conducted in March, September and December 2007 and February 2008, on four rocky shores located on São Sebastião Island, southeastern Brazil. A total of 1372 specimens of C. filosa were recorded, including 934 juveniles, 274 females (60 ovigerous) and 164 males. Population mean density ranged from 1.27 to 6.40 ind/g with higher mean values in March 2007 and lower in December 2007 and February 2008. The total body length of males varied from 4.11 to 20.75 mm and of females from 3.58 to 20.22 mm. No significant difference was detected between male and female body length. The overall sex ratio significantly differed from 1:1 proportion and was skewed toward females (0.60:1). Ovigerous females occurred in higher proportion in February 2008 and presented mean fecundity of 26.01 ± 1.84 eggs/female (mean ± se), with great variation in the number of eggs found in the marsupium (between 1 and 56). Fecundity increased significantly with increasing female body length. The C. filosa population structure and reproduction were similar to those of populations in other locations, in Brazil and abroad indicating that pollution by petrogenic hydrocarbons in the São Sebastião Channel did not affect the population parameters of C. filosa in this region. Savigny 181 Kützing 184 200 Island 137 C recorded 93 juveniles 27 60 (6 ovigerous 16 127 1.2 640 6 40 6.4 indg ind g 411 4 11 4.1 2075 20 75 20.7 358 3 58 3.5 2022 22 20.2 1: 0.601. 0601 0.60 . 0 (0.60:1) 2601 26 01 26.0 84 1.8 eggsfemale se, se , se) 56. 56 56) locations 18 13 9 2 ( 12 1. 64 6. 41 4. 207 7 20. 35 5 3. 202 601 0.601 060 0.6 (0.60:1 260 26. 8 06 0. (0.60: (0.60 (0.6 (0. (0

RESUMO Este estudo teve como objetivo avaliar aspectos populacionais e reprodutivos do anfípode ampitoídeo Cymadusa filosa Savigny, 1816 em uma região com poluição por hidrocarbonetos petrogênicos. Amostragens de frondes de Sargassum furcatum e da fauna associada foram realizadas em março, setembro e dezembro de 2007 e fevereiro de 2008, em quatro costões rochosos localizados na Ilha de São Sebastião, sudeste do Brasil. Um total de 1372 espécimes de C. filosa foi registrado, incluindo 934 juvenis, 274 fêmeas (60 ovígeras) e 164 machos. A densidade populacional média variou de 1,27 a 6,40 ind/g, com valores médios maiores em março de 2007 e menores em dezembro de 2007 e fevereiro de 2008. O comprimento corporal total dos machos variou de 4,11 a 20,75 mm e das fêmeas de 3,58 a 20,22 mm. Nenhuma diferença significativa foi detectada entre o tamanho do corpo de machos e fêmeas. A razão sexual geral diferiu significativamente da proporção de 1:1 e foi desviada para as fêmeas (0,60:1). Fêmeas ovígeras ocorreram em maior proporção em fevereiro de 2008 e apresentaram fecundidade média de 26,01 ± 1,84 ovos/fêmea (média ± erro padrão), com grande variação no número de ovos encontrados no marsúpio (entre 1 e 56). A fecundidade aumentou significativamente com o aumento do comprimento corporal das fêmeas. A estrutura populacional e a reprodução de C. filosa foram semelhantes às de populações de outras localidades no Brasil e no exterior, indicando que a poluição por hidrocarbonetos petrogênicos no Canal de São Sebastião não afetou os parâmetros populacionais de C. filosa nesta região. Savigny 181 200 137 C registrado 93 juvenis 27 60 (6 16 127 1,2 640 6 40 6,4 indg ind g ind/g 411 4 11 4,1 2075 20 75 20,7 358 3 58 3,5 2022 22 20,2 1: 0,601. 0601 0,60 . 0 (0,60:1) 2601 26 01 26,0 184 84 1,8 ovosfêmea fêmea padrão, padrão , padrão) 56. 56 56) exterior 18 13 9 2 ( 12 1, 64 6, 41 4, 207 7 20, 35 5 3, 202 601 0,601 060 0,6 (0,60:1 260 26, 8 06 0, (0,60: (0,60 (0,6 (0, (0

Abstract: Aim This study evaluates the reproduction and fecundity of Leporinus lacustris in upper Paraná River floodplain environments with the objectives to (i) recognize the germ cells of folliculogenesis and oogenesis; (ii) characterize reproductive phases; (iii) investigate oocyte diameter and evaluate if this species has group synchronous or asynchronous oocyte development and if spawning is total or batch; (iv) estimate batch fecundity; (v) establish the relationship between fecundity/ovary weight, standard length, and total weight; (vi) verify the reproductive areas of this species in the upper Paraná River floodplain. Methods Sampling was carried out using gillnets in rivers, and open and closed lagoons, in March, June, September, and December 2013, 2014, 2015, 2016, 2018, and 2019 and March and September 2017. A total of 442 females were sampled and 111 subsampled, each of which had an ovary removed and fixed in Bouin solution. A subsample of 20 ovaries in the spawning capable phase were fixed in a 4% buffered formaldehyde solution to estimate the diameter of the oocytes and fecundity. The ovaries were dehydrated, embedded in historesin, cut, stained, and evaluated as regards folliculogenesis, oogenesis, development phases, and reproduction areas. Results The germ cells recorded were differentiated oogonia, primary and secondary growth oocytes, fully-grown and mature oocytes, and post-ovulatory follicles that permitted the recognition of the reproductive phases. Oocyte diameter information revealed that the species has group synchronous oocyte development, determinate fecundity and batch spawning, and eliminates an average of 38,490 oocytes. Conclusions The fact that Leporinus lacustris is reproductively successful in the Baía River and in the Fechada, Guaraná, Patos, Garças, Ventura and Ressaco do Pau Véio lagoons strengthens the importance of the areas of environmental protection of the islands and lowlands of the Paraná River, Ilha Grande National Park and Ivinheima River State Park. Abstract i (i oogenesis ii (ii phases iii (iii iv (iv v (v fecundityovary weight length vi (vi rivers June 2013 2014 2015 2016 2018 201 2017 44 11 subsampled 2 4 dehydrated historesin cut stained oogonia fullygrown fully grown postovulatory post ovulatory 38490 38 490 38,49 Fechada Guaraná Patos Garças 1 3849 3 49 38,4 384 38,

Resumo: Objetivo Este estudo avaliou a reprodução e fecundidade de L. lacustris em ambientes da planície de inundação do alto rio Paraná, com os objetivos de: (i) reconhecer as células germinativas da foliculogênese e oogênese; (ii) caracterizar as fases reprodutivas; (iii) investigar o diâmetro dos oócitos e avaliar se esta espécie tem o desenvolvimento dos oócitos sincrônico em grupo ou assincrônico e se a desova é total ou parcelada; (iv) estimar a fecundidade do lote; (v) estabelecer a relação entre fecundidade/peso do ovário, comprimento padrão e peso total e (vi) verificar as principais áreas de reprodução desta espécie na planície de inundação do alto rio Paraná. Métodos As amostragens foram realizadas em rios, lagoas abertas e fechadas no alto rio Paraná, com redes de espera nos meses de março, junho, setembro e dezembro de 2013, 2014, 2015, 2016, 2018 e 2019 e março e setembro de 2017. Foram amostradas 442 fêmeas e subamostradas 111, das quais foi retirada uma subamostra do ovário e fixada em solução de Bouin. Uma subamostra de 20 ovários na fase apta a desova foi fixada em solução de formaldeído tamponado a 4%, para posteriormente, estimar o diâmetro dos oócitos e fecundidade. Resultados As células germinativas registradas foram: oogônias diferenciadas, oócitos de crescimento primário e secundário, oócitos de crescimento completo e maduros, e folículos pós-ovulatórios que permitiram reconhecer as fases reprodutivas. As informações do diâmetro dos oócitos revelou que a espécie tem desenvolvimento dos oócitos sincrônico em grupo, fecundidade determinada e desova parcial, e elimina no período de desova em média 38.490 oócitos. Conclusões Leporinus lacustris reprodutivamente bem sucedido no rio Baía e nas lagoas Fechada, do Guaraná, dos Patos, das Garças, do Ventura e do Ressaco do Pau Véio reforçando a importância das áreas de proteção ambiental das ilhas e várzeas do rio Paraná, Parque Nacional da Ilha Grande e Parque Estadual do rio Ivinheima. Resumo L Paraná i (i oogênese ii (ii reprodutivas iii (iii parcelada iv (iv lote v (v fecundidadepeso vi (vi rios junho 2013 2014 2015 2016 201 2017 44 111 Bouin 2 4 4% posteriormente diferenciadas secundário maduros pósovulatórios pós ovulatórios parcial 38490 38 490 38.49 Fechada Guaraná Patos Garças Ivinheima 11 3849 3 49 38.4 1 384 38.

Abstract: Background: Echinochloa colona (junglerice), a troublesome weed in rice, is resistant to 15 herbicide active ingredients. High temperatures are linked to reduction of herbicide efficacy. Objective: Evaluate growth and seed production of junglerice, after five generations of recurrent selection with sublethal dose of rice herbicides under heat stress. Methods: Junglerice plants previously subjected to recurrent selection with herbicides and heat stress for three cycles were exposed to further iterative cycles of selection with heat stress (45 °C) and sublethal dose of florpyrauxifen-benzyl (FPB, 0.125x), imazethapyr (0.125x), quinclorac (0.25x), and glufosinate (0.25x). Plant injury was evaluated 1 and 3 weeks after treatment. Panicle number; plant height; and dry biomass of shoot, panicles, and seeds were recorded. Seed number plant-1 and reproductive effort were estimated. Results: The joint effect of heat stress and sublethal dose of herbicides reduced the sensitivity of junglerice to all herbicides tested. Plants treated with FPB and quinclorac produced more shoot biomass and seed number after five generations. Heat-stressed plants treated with FPB produced more seeds than non-stressed plants. Imazethapyr reduced seed production. Heat-stressed junglerice treated with glufosinate produced fewer seeds than non-stressed plants. Conclusions: The joint effect of heat stress and sublethal dose of herbicides reduced junglerice sensitivity to the four rice herbicides tested after five generations. Heat stress and sublethal dose of the relatively recent auxinic herbicide florpyrauxifen-benzyl reduced junglerice sensitivity and increased seed production. Abstract Background , (junglerice) ingredients efficacy Objective Methods 45 (4 °C C florpyrauxifenbenzyl florpyrauxifen benzyl FPB, (FPB 0.125x, 0125x x 0.125x 0 125x 0.125x) (0.125x) 0.25x, 025x 0.25x 25x (0.25x) 0.25x. . treatment height panicles recorded plant1 plant- estimated Results Heatstressed stressed nonstressed non Conclusions (junglerice 4 ( (0.125x (0.25x

ABSTRACT Ceratitis capitata is a polyphagous species adapted to the most diverse hosts. The life cycle of C. capitata is influenced by the host species in which it develops, and information about its biological data in star fruit is scarce. This study aimed to characterize the biological cycle of C. capitata in star fruit and mango. Portions of 20 g were offered in Petri dishes lined with moistened filter paper to 20 first-instar larvae up to three hours of age. Evaluations were performed daily, quantifying the period and larval survival, pupal viability and mass (puparia weighed at 24 hours of age), longevity, egg-adult period, and fecundity. Larval survival ranged from 89.75% in star fruit to 94.25% in mango. Pupal viability and mass were higher in larvae fed mango. The length of the larval period was not affected by the food substrate. Total and daily fecundities were higher in females from larvae fed mango. Both fruits allowed the complete development of C. capitata, but mango is the most suitable host for its development. hosts C develops scarce 2 firstinstar first instar age puparia age, , age) longevity eggadult egg adult fecundity 8975 89 75 89.75 9425 94 25 94.25 substrate 897 8 7 89.7 942 9 94.2 89. 94.

RESUMO Ceratitis capitata é uma espécie polífaga, adaptando-se aos mais diversos hospedeiros. O ciclo de vida de C. capitata é influenciado pelas espécies hospedeiras em que se desenvolve, e informações sobre seus dados biológicos em carambola são escassas. Este estudo teve como objetivo caracterizar o ciclo biológico de C. capitata nas frutas carambola e manga. Porções de 10 g foram oferecidas em placas de Petri forradas com papel filtro umedecido para 20 larvas de primeiro ínstar com até três horas de idade. As avaliações foram realizadas diariamente, quantificando-se o período e sobrevivência larval, viabilidade e massa pupal (pupários pesados às 24 horas de idade), longevidade, período ovo-adulto e fecundidade. A sobrevivência larval variou de 89,75% em carambola a 94,25% em manga, a viabilidade e a massa pupal foram maiores em larvas alimentadas com manga. A duração do período larval não foi afetada pelo substrato alimentar. As fecundidades total e diária foram maiores nas fêmeas de larvas alimentadas com manga. Ambas as frutas testadas permitiram o desenvolvimento completo de C. capitata, mas a manga é o hospedeiro mais adequado para seu desenvolvimento. polífaga adaptandose adaptando hospedeiros C desenvolve escassas 1 2 idade diariamente quantificandose quantificando pupários idade, , idade) longevidade ovoadulto ovo adulto fecundidade 8975 89 75 89,75 9425 94 25 94,25 alimentar 897 8 7 89,7 942 9 94,2 89, 94,

Abstract: One of the challenges of the Kantian research is to deal with the different meanings of the term critique, as well as to ask about the author’s possible motivation in choosing this term to title his main works. Through the emphasis on the fecundity of meaning of the term critique, we will show in this article that its connection with the establishment of limits to metaphysics was a sinuous process, which is capable of encompassing interests of heterogeneous natures. For this purpose, we will first problematize, in particular, the appearance of the concept of critique in NEV of 1765 and, later, based on the Reflections (1765-1770), we will identify how its equivocality, more than an obstacle, favored the construction of its protagonist role in the Kantian corpus. Thus, the critical thinking for Kant, instead of a position facing only the problem of metaphysics, is explained as a position facing the most diverse problems of the modern subject. Abstract authors author s works process natures purpose problematize particular 176 and later 17651770, 17651770 1770 , (1765-1770) equivocality obstacle corpus Thus Kant subject 17 1765177 177 (1765-1770 1 176517 (1765-177 17651 (1765-17 (1765-1 (1765- (1765 (176 (17 (1 (

Resumo: Um dos desafios do comentário à filosofia kantiana é lidar com as possíveis acepções do termo “crítica”, juntamente com a possível motivação do autor na escolha desse termo, para intitular suas principais obras. Neste artigo, enfatizando a fecundidade de significado do termo “crítica”, mostra-se que a sua ligação com o estabelecimento de limites à metafísica foi um processo sinuoso, o qual perpassou preocupações de naturezas heterogêneas. Para tanto, problematiza-se, primeiramente, o aparecimento do conceito de crítica, em especial, em NEV de 1765 e, posteriormente, com base nas Reflexões até 1770, identifica-se como a sua equivocidade, mais do que um empecilho, favoreceu a construção de seu protagonismo, no corpus kantiano. Assim, o pensamento crítico em Kant, em vez de uma posição diante do problema da metafísica, explicita-se como uma posição diante dos mais diversos problemas do sujeito moderno. Resumo crítica , “crítica” obras artigo mostrase mostra se sinuoso heterogêneas tanto problematizase, problematizase problematiza se, problematiza-se primeiramente especial 176 e posteriormente 1770 identificase identifica equivocidade empecilho protagonismo kantiano Assim Kant explicitase explicita moderno “crítica 17 177 1

Abstract As a positional and geometrical isomer of linoleic acid, trans 10, cis 12 conjugated linoleic acid (t10c12-CLA) reduces white fat by reducing food intake, modulating lipid metabolism, and stimulating energy expenditure. However, the t10c12-CLA products are mostly mixtures, making it difficult to obtain accurate results. Studies are needed to investigate the effects of pure t10c12-CLA on animals and humans. In this study, we used the biallelic transgenic (tg) mice, which could produce t10c12-CLA itself, to investigate the effects of pure t10c12-CLA on female reproductive ability. The results showed that the body and relative ovary weights had no significant difference between tg and wild-type (wt) littermates at ages 3 or 10 weeks. While the fecundity test found that tg mice had a significantly longer first litter time (32.0 ± 4.70 days vs. 21.3 ± 2.31 days, P<0.05), and a significantly lower number of litters (4.75 ± 2.75 vs. 6.67 ± 0.57, P<0.05) when compared with wt mice during continuous mating within seven months. Hormone profiles showed that serum estradiol levels did not change in tg mice; however, significantly (P<0.05) decreased progesterone and increased prostaglandin E2 levels were observed in tg mice compared with those of wt mice. Hematoxylin-eosin staining showed no pathological characteristics in tg ovaries, except for the increased atresia follicles (P<0.05). Moreover, the tg mice had a significantly more extended diestrus period than the wt mice (48.4 ± 6.38% vs. 39.6 ± 3.81%, P<0.05). In summary, t10c12-CLA could affect serum progesterone and prostaglandin E2 levels, lead to a disordered estrus cycle, and impact the reproductive performance of female mice. This study provided theoretical and biosafety recommendations for applying t10c12-CLA in female mammals. 1 t10c12CLA tcCLA t10c12 CLA t c (t10c12-CLA intake metabolism expenditure However mixtures humans (tg itself ability wildtype wild type (wt weeks 32.0 320 32 0 (32. 470 4 70 4.7 vs 213 21 21. 231 2 31 2.3 P<0.05, P005 P P<0.05 , 05 4.75 475 75 (4.7 275 2.7 667 6 67 6.6 057 57 0.57 months however (P<0.05 E Hematoxylineosin Hematoxylin eosin ovaries P<0.05. . Moreover 48.4 484 48 (48. 638 38 6.38 396 39 39. 381 81 3.81% summary cycle mammals tc t10c1 32. (32 47 7 4. 23 2. P00 P<0.0 (4. 27 66 6. 5 0.5 (P<0.0 48. (48 63 6.3 8 3.81 t10c (3 P0 P<0. (4 0. (P<0. 3.8 ( P<0 (P<0 3. P< (P< (P

ABSTRACT The soybean cyst nematode (Heterodera glycines) may result in a significant economic impact on bean cultivation. This study aimed to evaluate the reaction of 55 common bean genotypes from the Embrapa Arroz e Feijão core collection to the race 3/HG type 0 of H. glycines. Among the genotypes evaluated, 34 were considered resistant to the cyst nematode, and, in addition to showing a lower population of females in the roots, also reduced the fecundity of H. glycines. Heterodera glycines cultivation 5 3HG HG 3 H evaluated and roots

RESUMO O nematoide de cisto da soja (Heterodera glycines) pode resultar em impacto econômico significativo no cultivo de feijão. Objetivou-se avaliar a reação de 55 genótipos de feijão comum da coleção nuclear da Embrapa Arroz e Feijão à raça 3/Tipo HG 0 de H. glycines. Dentre os genótipos avaliados, 34 foram considerados resistentes ao nematoide de cisto, os quais, além de apresentarem menor população de fêmeas nas raízes, também reduziram a fecundidade de H. glycines. Heterodera glycines Objetivouse Objetivou se 5 3Tipo Tipo 3 H avaliados quais raízes

ABSTRACT The ‘Tahiti’ acid lime and orange trees are hosts of ‘Candidatus Liberibacter asiaticus’ (CLas), the pathogen associated with the severe Asian form of huanglongbing (HLB), the most devasting citrus disease. They are also hosts of the vector of CLas, the Asian citrus psyllid (ACP) Diaphorina citri Kuwayama. Relatively small numbers of lime trees occur in gardens and small orchards near large commercial ‘Valencia’ sweet orange orchards in Brazil. Applications of insecticides to suppress populations of ACP on the lime trees are usually nil or less frequent than in the orange orchards. Abundance of the psyllid on lime trees may therefore increase the risk of CLas spreading to the orchards. Because the abundance of the psyllid is influenced by the suitability of the trees as hosts, we compared reproductive potential of the insect on the two hosts in a controlled environment chamber (CEC) and in a greenhouse (GH). Daily temperature and relative humidity averaged 22ºC and 60% inside the CEC and 24°C and 70% inside the GH. Two pairs of adult male and female psyllids were caged for 3 days on new shoots and the fecundity and durations of development and survival of eggs and nymphs evaluated. Overall, acid ‘Tahiti’ was 3.5 times less suitable to ACP than ‘Valencia’. Fecundity and survival of nymphs were 27% and 59% lower, and the life cycle 34% longer on ‘Tahiti’ than on ‘Valencia’. Potential impacts of the results on CLas spread and HLB control are discussed. ‘Tahiti Tahiti Candidatus asiaticus , (CLas) HLB, (HLB) disease (ACP Kuwayama ‘Valencia Valencia Brazil (CEC GH . (GH) ºC 60 24C C 24 70 evaluated Overall 35 5 3. ‘Valencia. 27 59 lower 34 discussed (CLas (HLB (GH 6 2 7

ABSTRACT The significant role of Aedes aegypti as a vector of several arboviruses has intensified studies on vector control tools, including Attractive Toxic Sugar Baits containing Pyriproxyfen (ATSB-PPF). PPF is an insect growth regulator (IGR) that has been used to control immature mosquitoes, but it also has direct effects on female reproduction. This study evaluated the effect of physical exposure of Ae. aegypti females with ATSB-PPF on fecundity and fertility at different times before and after blood meal. Females were confined in cages impregnated with ATSB-PPF at different concentrations (5, 50, and 500 ppm). One group of females was exposed to the baits 24 and 48 hours Before the Blood Meal (BBM), and in the other group, exposure occurred 24 and 48 hours After the Blood Meal (ABM). The number of eggs laid, larvae, and pupation rate were analyzed. Exposure of Ae. aegypti females to ATSB-PPF bait 24 hours after the blood meal was able to reduce the number of eggs laid, especially at the higher concentrations used, but the number of larvae obtained was reduced regardless of the exposure time to ATSB-PPF, i.e., 24 hours before or after the blood meal, or the concentration of PPF used. No changes were observed in the number of pupae from larvae obtained from eggs laid by mosquitoes exposed to ATSB-PPF. Physical exposure of mosquitoes to sugar baits is sufficient to reduce the investigated reproductive parameters of Ae. aegypti. tools ATSBPPF. ATSBPPF ATSB . (ATSB-PPF) IGR (IGR reproduction Ae 5, 5 (5 50 ppm. ppm ppm) 2 4 BBM, BBM , (BBM) ABM. ABM (ABM) analyzed ATSBPPF, PPF, ie i e i.e. PPF. (ATSB-PPF ( (BBM (ABM i.e

Resumo Este trabalho é uma revisão integrativa que visa levantar a produção bibliográfica em periódicos de artigos que explorem a intersecção entre o pensamento pedagógico de Paulo Freire e os arcabouços teóricos da psicanálise de Sigmund Freud. O levantamento ocorreu no segundo semestre do ano de 2022, em múltiplos repositórios, tanto os de caráter geral (Scientific Electronic Library Online/SciELO e Portal de Periódicos da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/CAPES), quanto as bases específicas das áreas da Educação (Associação Nacional de Pós-Graduação e Pesquisa em Educação/ANPEd e EDUC@) e da Psicologia (Portal de Periódicos Eletrônicos de Psicologia/PePSIC), a partir da combinação dos descritores “Freire” e “Freud” com o descritor “psicanálise”. Das centenas de documentos recuperados, após leitura e aplicação de critérios de exclusão, elegemos oito para compor o corpo de análise deste trabalho. As dificuldades e entraves no levantamento bibliográfico evidenciaram a carência de pesquisas sobre a interlocução entre estes autores, apesar de sua aparente fecundidade. O cotejamento dos escritos apontou similitudes entre as teorias dos dois autores, que convergem em pontos fulcrais de seus trabalhos. Conceitos freudianos como narcisismo, sadismo e masoquismo indicam processos psíquicos de colonização que podem manter os oprimidos em posição de passividade ou até de identificação perante o opressor. Entender estes mecanismos psíquicos por intermédio do corpo de conhecimento transdisciplinar que é a psicanálise pode oferecer bases para a compreensão e para a luta contra os grilhões físicos e psicológicos que acorrentam o oprimido. Freud 2022 repositórios Scientific OnlineSciELO Online SciELO Superior/CAPES, SuperiorCAPES Superior/CAPES , Superior CAPES Superior/CAPES) Associação PósGraduação Pós Graduação EducaçãoANPEd ANPEd EDUC@ EDUC Psicologia/PePSIC, PsicologiaPePSIC Psicologia/PePSIC PePSIC Psicologia/PePSIC) “Freire “Freud psicanálise. . “psicanálise” recuperados exclusão autores fecundidade trabalhos narcisismo opressor oprimido 202 “psicanálise 20 2

Abstract This paper is an integrative review that surveys the bibliographic production in academic journals that explore the intersection between Paulo Freire’s pedagogical thought and Sigmund Freud’s psychoanalytic framework. The survey was conducted in the second half of 2022 across multiple repositories, both general ones (Scientific Electronic Library Online/SciELO and the Portal de Periódicos of the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/CAPES), as well as specific databases in the fields of Education in Brazil (Associação Nacional de Pós-Graduação e Pesquisa em Educação/ANPEd and EDUC@) and Psychology (Portal de Periódicos Eletrônicos de Psicologia/PePSIC), using the combination of the descriptors “Freire” and “Freud” with the descriptor “psychoanalysis.” From the hundreds of documents retrieved, after reading and applying exclusion criteria, we selected eight to comprise the body of analysis for this work. The difficulties and obstacles encountered in the bibliographic survey highlighted the scarcity of research on the dialogue between these authors, despite its apparent fecundity. The comparison of the writings indicated similarities between the theories of the two authors, which converge on crucial points of their works. Freudian concepts such as narcissism, sadism, and masochism suggest psychic processes of colonization that can keep the oppressed in a position of passivity or even identification with the oppressor. Understanding these psychic mechanisms through the transdisciplinary body of knowledge of psychoanalysis may provide foundations for the understanding of and the fight against the physical and psychological chains that bind the oppressed. Freires Freire s Freuds Freud framework 202 repositories Scientific OnlineSciELO Online SciELO Superior/CAPES, SuperiorCAPES Superior/CAPES , Superior CAPES Superior/CAPES) Associação PósGraduação Pós Graduação EducaçãoANPEd Educação ANPEd EDUC@ EDUC Psicologia/PePSIC, PsicologiaPePSIC Psicologia/PePSIC Psicologia PePSIC Psicologia/PePSIC) “Freire “Freud psychoanalysis. “psychoanalysis. retrieved criteria work authors fecundity works narcissism sadism oppressor 20 “psychoanalysis 2