Human milk (HM) is recognized as an ideal nutritional source to meet the developmental needs of babies. However, a significant portion does not meet quality standards for immediate consumption, rendering it an interesting raw material for the production of by-products. This study aimed to evaluate the lipid composition and cytokines interleukin-10 (IL-10) and transforming growth factor-beta 1 (TGF-β1) in ice cream containing HM. Three formulations were developed: F1 with 93.46% HM, F2 with 46.73% cows’ milk and 46.73% HM, and F3 with 46.73% infant formula and 46.73% HM. Fatty acids (FA) and their nutritional quality, the triacylglycerol (TAG) profile, and the concentrations of cytokines IL-10 and TGF-β1 were analyzed. F1 and F3 exhibited the highest concentrations of essential FAs (18:2n-6 and 18:3n-3) compared to F2. This trend extended to eicosapentaenoic acid + docosahexaenoic acid, with higher values in F1 (10.62 ± 0.26) and F3 (11.16 ± 0.28) versus F2 (3.67 ± 0.18). Both cytokines IL-10 and TGF-β1, present in the ice creams, experienced a decrease in concentration due to the processing of the raw materials. Therefore, ice creams produced with HM have promising potential, making them recommended for use, providing a new destination for waste, and creating a novel product that can be used as a complementary food.

RESUMO O presente estudo teve como objetivo investigar a influência de microalgas utilizadas no cultivo em água verde na taxa de sobrevivência, no desempenho e no perfil de ácidos graxos de larvas de H. reidi. O estudo consistiu de quatro tratamentos compostos por duas microalgas (Tisochrysis lutea - ISO e Chaetoceros muelleri-CHO), utilizadas isoladamente (TISO e TCHO) ou combinadas (TIC 1:1) na água de cultivo, e um tratamento sem microalgas (TWM) durante os primeiros 15 dias de cultura. As larvas foram alimentadas do primeiro ao sétimo dia com copépodes (Parvocalanus crassirrostris) e rotíferos (Brachionus rotundiformis), e, a partir do oitavo dia, ocorreu a inclusão gradual de Artemia sp. náuplios. Não foram observadas diferenças nas taxas de sobrevivência. A adição específica de T. lutea nos tratamentos TISO e TIC afetou o peso, o ganho de peso, a taxa de crescimento específico e o fator de condição das larvas em comparação ao TWM. Uma maior concentração de (PUFA), DHA, proporção ∑ n-3, n-3/n-6, foi encontrada em larvas de TISO após 15 dias de criação. Conclui-se que a adição da microalga T. lutea mostrou-se uma alternativa vantajosa, uma vez que sua inclusão afetou o desempenho e a composição lipídica das larvas em comparação ao TWM.

ABSTRACT The study aimed to investigate the influence of different microalgae used in green water culture on the survival rate, performance and fatty acids profile of H. reidi larvae. The study consisted of four treatments composed of two microalgae (Tisochrysis lutea - ISO and Chaetoceros muelleri-CHO), used alone (TISO and TCHO) or combined (TIC, 1:1) in the culture water, and a treatment without microalgae (TWM) during the first 15 days of culture. The larvae were fed from the first to the seventh day with copepods (Parvocalanus crassirrostris) and rotifers (Brachionus rotundiformis), and from the eighth day onwards, the gradual inclusion of Artemia sp. nauplii. No differences in the survival rates were observed. The addition of specifically T. lutea in the TISO and TIC treatments affected weight, weight gain, specific growth rate and the condition factor, of the larvae compared to TWM. A higher concentration of (PUFA), DHA, ∑ n-3, n-3/n-6 ratio were found in TISO larvae after 15 days of rearing. In conclusion, the addition of microalgae T. lutea proved to be an advantageous alternative in a large-scale production system, since its inclusion affected the performance and the lipid composition of the larvae compared to the TWM.

RESUMO Foi realizado o do perfil bioquímico e das gorduras/proteínas do leite em cabras Saanen e Nubian de Los Asmoles, Colima. Gordura do leite, proteína do leite, relação gordura/proteína, glicose (GLU), colesterol (COL), ácidos graxos não esterificados (NEFA), triacilglicerol (TAG), β-hidroxibutirato (β-HBA), nitrogênio ureico no sangue (BUN), albumina (ALB), globulina (GLOB), proteína total (PROT-T), íon cálcio (Ca2+), fosfato inorgânico (Pi), íon sódio (Na+), íon potássio (K+), íon magnésio (Mg2+) e íon cloreto (Cl-) foram calculados. Os resultados do perfil bioquímico foram consistentes com a literatura internacional para caprinos. Todas as análises, exceto ALB e β-HBA, mostraram diferenças raciais. As três variáveis lácteas registraram maior concentração no Nubian. A comparação entre os grupos (alto rendimento vs. baixo rendimento vs. período seco) mostrou diferenças em COL e β-HBA em ambas as raças. Uma correlação negativa entre GLU e NEFA foi quantificada. Foram quantificadas correlações positivas entre NEFA com COL, TAG e β-HBA, entre Ca2+ com Na+ e entre K+ com Na+ e Mg2+. A gordura do leite foi correlacionada com COL, NEFA e β-HBA. A proteína do leite foi correlacionada com a BUN. A relação gordura/proteína foi correlacionada com COL e β-HBA. Os resultados fornecem informações sobre as adaptações metabólicas na lactação e identificam ligações entre as principais análises e gordura/proteína do leite em cabras.

ABSTRACT The biochemical profile and milk fats/proteins in Saanen and Nubian goats from Los Asmoles, Colima was carried out. Milk fat, milk protein, fat/protein ratio, glucose (GLU), cholesterol (COL), non-esterified fatty acids (NEFA), triacylglycerol (TAG), β-hydroxybutyrate (β-HBA), blood urea nitrogen (BUN), albumin (ALB), globulin (GLOB), total protein (PROT-T), calcium ion (Ca2+), inorganic phosphate (Pi), sodium ion (Na+), potassium ion (K+), magnesium ion (Mg2+), and chloride ion (Cl-) was calculated. The biochemical profile results were consistent with the international literature for goats. All analytes except ALB and β-HBA showed racial differences. The three milk variables registered a higher concentration in Nubian. The comparison between groups (high-yield vs. low-yield vs. dry period) showed differences in COL and β-HBA in both breeds. A negative correlation between GLU and NEFA was quantified. Positive correlations between NEFA with COL, TAG and β-HBA, between Ca2+ with Na+ and between K+ with Na+ and Mg2+ were quantified. Milk fat was correlated with COL, NEFA and β-HBA. Milk protein was correlated with BUN. The fat/protein ratio was correlated with COL and β-HBA. Results provide insight into metabolic adaptations in lactation and identify links between key analytes and milk fat/protein in goats.

Evening primrose oil supplements are rich in omega-6 fatty acids, with emphasis on linoleic and γ-linolenic acids, being taken in the treatment and prevention of diseases, but mainly for the relief of symptoms caused by premenstrual tension and menopause in women. This study aimed to evaluate the quality of the lipid profile of evening primrose oil supplements using gas chromatography techniques with a flame ionization detector and mass spectrometry with electrospray ionization. For this, eight commercially available national evening primrose oil brands were used. Of the eight samples analyzed, only two met all the standards established in legislation for fatty acids. The remaining capsules were subjected to triacylglycerol analysis and using a calibration curve the presence of soybean oil in the composition of the evening primrose oil capsules was verified. From the triacylglycerol with a mass/charge ratio of 901, it was possible to identify the addition of 31.16-85.88% of soybean oil in six of the eight capsules, leading the consumer to ingest products that did not comply with current legislation. omega6 omega 6 omega- acids γlinolenic γ linolenic diseases women this used analyzed verified masscharge charge 901 31.1685.88% 31168588 31.16 85.88% 31 16 85 88 31.16-85.88 90 1685 31.1685.88 3116858 3116 31.1 8588 85.88 3 1 8 31.16-85.8 9 168 31.1685.8 311685 311 31. 858 85.8 31.16-85. 31.1685. 31168 85. 31.16-85 31.1685 31.16-8 31.168 31.16-

We report a facile hydrothermal method for the synthesis of niobium pentoxide (Nb2O5) under two different temperatures, 120 °C (Nb2O5-120) and 150 °C (Nb2O5-150). The obtained materials were characterized by structural, optical and morphological techniques. Also, the photocatalytic properties of the Nb2O5 samples were evaluated in two reactions under ultraviolet (UVC) irradiation: degradation of methylene blue (MB) and indigo carmine (IC) dyes; and esterification of free fatty acids (FFA) present in waste cooking oil (WCO). The Nb2O5 was formed at different temperatures of synthesis, and the increase in temperature did not cause significant changes in the structural and optical characteristics, but resulted in an increased surface area of the synthesized materials. Both synthesized materials showed excellent efficiency for dye photodegradation. The sample Nb2O5-150 presented the best performance for the MB photodegradation, with almost 85% of the removal efficiency. In this case, the adsorption of MB molecules on the surface of the material was high due to the favorable electrostatic interaction and also because of its high surface area. For the IC photodegradation, the adsorption was insignificant, and both samples presented approximately 100% of the removal efficiency. These materials were also promising for the reduction of free fat acids in waste oil by photoesterification. NbO Nb O (Nb2O5 12 C Nb2O5120 (Nb2O5-120 15 Nb2O5150. Nb2O5150 . (Nb2O5-150) techniques Also Nb2O UVC (UVC irradiation (MB (IC dyes FFA (FFA WCO. WCO (WCO) characteristics photodegradation Nb2O5-15 85 case insignificant 100 photoesterification (Nb2O 1 Nb2O512 (Nb2O5-12 Nb2O515 (Nb2O5-150 (WCO Nb2O5-1 8 10 Nb2O51 (Nb2O5-1 (Nb2O5-15 Nb2O5- (Nb2O5-

This study describes the chemical composition and biological activities of extracts and compounds obtained from the roots of Bowdichia virgilioides (Fabaceae). A mixture of very long-chain free fatty acids (VLFAs) and pure compounds, namely isocordoin, cordoin, lupeol, lupenone, β-sitosterol, stigmasterol, and sitostenone, were isolated from the soluble fraction of the methanol extract by using sequential column chromatography procedures. The molecular structures of the compounds were determined using infrared (IR), nuclear magnetic resonance (NMR), and mass spectrometry (MS) techniques. Notably, this is the first report of the occurrence of both chalcones within this plant genus. Besides, the VLFAs were identified by electrospray ionization mass spectrometry (ESIMS) and the presence of these compounds in roots is suggested to be an adaptive response to environmental stress, which exhibited moderate antioxidant properties when tested against the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and in the β-carotene/linolenic acid auto-oxidation assay. However, the MeOH soluble fraction demonstrated significant toxicity in the brine shrimp lethality assay. Fabaceae. Fabaceae . (Fabaceae) longchain long chain (VLFAs isocordoin cordoin lupeol lupenone βsitosterol, βsitosterol β sitosterol, sitosterol β-sitosterol stigmasterol sitostenone procedures IR, IR , (IR) NMR, NMR (NMR) MS (MS techniques Notably genus Besides ESIMS (ESIMS stress 2,2diphenyl1picrylhydrazyl 22diphenyl1picrylhydrazyl diphenylpicrylhydrazyl 2,2 diphenyl 1 picrylhydrazyl 2 DPPH (DPPH βcarotene/linolenic βcarotenelinolenic carotene/linolenic carotene linolenic autooxidation auto oxidation assay However (Fabaceae (IR (NMR 2diphenyl1picrylhydrazyl 22 2, βcarotene carotenelinolenic

Brazilian Savanna biodiversity is abundant in native fruits with nutritional and sensory properties with potential technological applications. However, the information available regarding the proprieties of Brazilian native fruits is still limited. This study aimed to analyze the metabolic profile of Brosimum gaudichaudii fruits (BGF) using metabolomics approaches, besides its physicochemical and nutritional proprieties. BGF exhibited protein content at least two times higher than other native fruits. Fifty-five metabolites were identified by the metabolomics approaches, mainly fatty acids (n = 22), carbohydrates (n = 13), organic acids (n = 6), and polyphenols (n = 5). The most abundant constituents were D-fructose (42.97%) for sugars, L-asparagine (6.47%) for amino acids, succinic acid (44.11%) for organic acids, and linolenic acid (3.04%) for fatty acids. Highlighted phytochemicals included chlorogenic acid, α-amyrin, and γ-tocopherol. Additionally, this study revealed that BGF is a good source of vitamin C and dietary fiber and an excellent source of vitamin A. Future studies providing nutritional information and suggesting consumption methods and potential industrial applications are essential to familiarize consumers with these foods. Additionally, clarifying the appropriate planting and harvesting seasons can revive traditional practices, especially for native fruits like BGF. However limited (BGF approaches Fiftyfive Fifty five n 22, 22 , 22) 13, 13 13) 6, 6 6) 5. 5 . 5) Dfructose D fructose 42.97% 4297 42 97 (42.97% sugars Lasparagine L asparagine 6.47% 647 47 (6.47% 44.11% 4411 44 11 (44.11% 3.04% 304 3 04 (3.04% αamyrin, αamyrin α amyrin, amyrin α-amyrin γtocopherol. γtocopherol γ tocopherol. tocopherol γ-tocopherol Additionally A foods practices 2 1 42.97 429 4 9 (42.97 6.47 64 (6.47 44.11 441 (44.11 3.04 30 0 (3.04 42.9 (42.9 6.4 (6.4 44.1 (44.1 3.0 (3.0 42. (42. 6. (6. 44. (44. 3. (3. (42 (6 (44 (3 (4 (

Gas chromatography coupled to mass spectrometry (GC-MS) has been widely used, and along with other methodologies such as derivatization, is a powerful tool to analyze phytocompounds, including cuticular wax components. The loss of structural information upon fragmentation of waxes makes it difficult to interpret and identify the GC-MS mass spectra of these compounds with the traditional methods, as digital libraries. Therefore, as a new complementary alternative to traditional methods of identifying aliphatic acyclic lipids based on mass spectra, we present the new software WaxAlly in this technical note. WaxAlly is a new software created based on simple algorithms, enabling the rapid recognition by the user of eight aliphatic acyclic lipid classes, including alkanes, alkenes, aldehydes, ketones, esters, and TMS derivatives of free fatty acids, primary and secondary alcohols, and their homologues with carbon chains varying between 10 and 100 carbons. Additionally, the WaxAlly software provides a section for data organization, internet comparison to NIST and PubChem databases, and academic information about mass fragmentation of acyclic lipids. The software has proven to be a very useful complementary technique in identifying plant wax lipid homologues, and new fragmentation patterns of lipid classes can be added in the future to improve the program. GCMS GC MS (GC-MS used derivatization phytocompounds components libraries Therefore note algorithms alkanes alkenes aldehydes ketones esters acids alcohols 1 carbons Additionally organization databases program

Brazil is home to a rich biodiversity of native edible fruits renowned for their health-promoting compounds and flavors. Among these, Eugenia uniflora L. (Brazilian pitanga) is particularly notable. The fruit varies in color from yellow to purple and is prized for its intense aroma and versatile taste profile. However, information about the compounds responsible for their nutritional and sensory potential is still limited. This study analyzed volatile compounds and metabolites in yellow, red and purple pitanga fruits (Eugenia uniflora) at various ripening stages, exploiting a metabolomics approach. Yellow pitanga had 30 volatile compounds, with esters (40%), terpenes (26.67%) and aldehydes (16.67%) constituting 83.34% of identified volatile compounds. Red pitanga featured 26 volatile compounds, primarily terpenes and esters (each 30.77%) and aldehydes (15.38%), making up 76.92%. Purple pitanga contained 39 volatile compounds, with terpenes and esters (each 35.90%) and aldehydes (17.95%), totaling 89.75% of identified volatile compounds. Across all pitanga types, 124 metabolites were identified, including saturated fatty acids (17.74%), terpenes (17.74%) and sugars (16.93%), constituting 52.41% of identified metabolites. Future studies should provide nutritional information, consumption methods and industrial applications for pitanga fruits, focusing on sensory analysis, health benefits, preservation and breeding to enhance bioactive compounds and flavor. healthpromoting promoting flavors these L Brazilian notable profile However limited stages approach 3 40%, 40 40% , (40%) 26.67% 2667 67 (26.67% 16.67% 1667 16 (16.67% 8334 83 34 83.34 2 each 30.77% 3077 77 15.38%, 1538 15.38% 15 38 (15.38%) 7692 76 92 76.92% 35.90% 3590 35 90 17.95%, 1795 17.95% 17 95 (17.95%) 8975 89 75 89.75 types 12 17.74%, 1774 17.74% 74 (17.74% 16.93%, 1693 16.93% 93 (16.93%) 5241 52 41 52.41 analysis benefits flavor 4 (40% 26.67 266 6 (26.67 16.67 166 1 (16.67 833 8 83.3 30.77 307 7 153 15.38 (15.38% 769 9 76.92 35.90 359 179 17.95 (17.95% 897 89.7 177 17.74 (17.74 169 16.93 (16.93% 524 5 52.4 (40 26.6 (26.6 16.6 (16.6 83. 30.7 15.3 (15.38 76.9 35.9 17.9 (17.95 89. 17.7 (17.7 16.9 (16.93 52. (4 26. (26. 16. (16. 30. 15. (15.3 76. 35. 17. (17.9 (17. (16.9 ( (26 (16 (15. (17 (2 (1 (15

ABSTRACT Proper waste management is a major worldwide concern; waste is disposed of directly into the environment, affecting water, soil and air quality. The development and production of green energy sources is strongly encouraged in modern society. The aim of this study, in accordance with Sustainable Development Goals (SDGs) 1, 2 and 7 of the 2030 Agenda of the UN, is to combine the correct disposal of animal waste with the generation of energy to mitigate the energy insecurity of families in an extremely vulnerable semi-arid region in the north-east of the country using biodigesters. To this end, a conceptual proposal was drawn up for a low-cost, easy-to-maintain and easy-to-use biodigester. A small-scale prototype was used to assess its operation by evaluating the main factors that affect biogas production (pH, temperature and the biomass properties: total solids, fixed solids, volatile solids, concentration of volatile fatty acids and C/N ratio). Evaluation of the test prototype gave satisfactory results that corroborate other studies and make it possible to propose an economically viable and efficient full-scale biodigester for biogas production. concern environment water quality society study SDGs (SDGs 1 203 UN semiarid semi arid northeast north east biodigesters end lowcost, lowcost low cost, cost low-cost easytomaintain easy maintain easytouse use smallscale small scale pH, pH (pH properties solids CN C N ratio. ratio . ratio) fullscale full 20

Bredemeyera Willd. is a genus of the Polygalaceae family, occurring in Brazil. This research aims to evaluate the phytochemical and antifungal potential, in vitro and in silico, of Bredemeyera brevifolia extracts. The identification of fatty acids by gas chromatography-mass spectrometry and their quantification by gas chromatography-flame ionization detector showed that palmitic (36.55 mg g−1), margaric (3.66 mg g−1), linolelaidic (108.82 mg g−1), oleic (7.86 mg g−1), stearic (41.11 mg g−1), dihomo-γ-linolenic (85.48 mg g−1), and arachidic (209.00 mg g−1) acids are present in the hexane extract. From the chloroform extract, 1-hydroxy-3,7-dimethoxyxanthone was isolated and identified. The minimum inhibitory concentration assay with methanolic extract demonstrated antifungal activity against species of the genus Candida, Sporothrix, and Trichophyton for up to 96 h, with values ranging from 64-1024 μg mL−1. The in silico study with the chemical compounds of this species showed fungal inhibition potential of sterol-14-α-demethylase, highlighting 1-hydroxy-3,7-dimethoxyxanthone, which presented an affinity energy of -8.8 kcal mol−1, greater than that observed for fluconazole (−8.3 kcal mol−1). The fatty acids interacted with the active site of the enzyme but presented energies ≥ −7.4 kcal mol−1. Compounds isolated from this species have pharmacokinetic potential for the development of pharmaceutical formulations. Willd family Brazil extracts chromatographymass chromatography mass chromatographyflame flame 36.55 3655 36 55 (36.5 g−1, g1 g g−1 , 1 3.66 366 3 66 (3.6 108.82 10882 108 82 (108.8 7.86 786 7 86 (7.8 41.11 4111 41 11 (41.1 dihomoγlinolenic dihomo γ linolenic 85.48 8548 85 48 (85.4 209.00 20900 209 00 (209.0 1hydroxy3,7dimethoxyxanthone 1hydroxy37dimethoxyxanthone hydroxydimethoxyxanthone hydroxy 3,7 dimethoxyxanthone identified Candida Sporothrix 9 h 641024 64 1024 64-102 mL1 mL mL−1 sterol14αdemethylase, sterol14αdemethylase sterolαdemethylase sterol 14 α demethylase, demethylase sterol-14-α-demethylase 1hydroxy3,7dimethoxyxanthone, dimethoxyxanthone, 8.8 88 8 -8. mol1 mol mol−1 −8.3 83 (−8. . mol−1) 74 4 −7. formulations 36.5 365 5 (36. g− 3.6 6 (3. 108.8 1088 10 (108. 7.8 78 (7. 41.1 411 (41. 85.4 854 (85. 209.0 2090 20 0 (209. 1hydroxy3 7dimethoxyxanthone 37 3, 64102 102 64-10 mL− αdemethylase 8. -8 mol− −8. (−8 −7 36. (36 3. (3 108. (108 7. (7 41. (41 85. (85 209. 2 (209 1hydroxy 6410 64-1 - −8 (− − ( (10 (4 (8 (20 641 64- (1 (2

Exploring the triacylglycerols composition in milk samples from different ruminant species is crucial for dairy product development. Identifying the fatty acid (FA) composition in human milk is essential and is usually performed using gas chromatography (GC). Heat maps are useful for visualizing these results, aiding in pattern identification and relationships between samples and variables. Additionally, nuclear magnetic resonance (NMR) spectroscopy provides important qualitative and quantitative information about fatty acids (FA). In the present study, we compare the fatty acid composition in samples of HM, cow milk, and goat milk, as well as to demonstrate their positional distribution in triacylglycerols (TAGs). Human milk contains 49.21% saturated fatty acids (SFAs), 34.32% monounsaturated fatty acids (MUFA), and 16.47% polyunsaturated fatty acids (PUFA), including 2.14% docosahexaenoic acid (DHA) and 13.02% linoleic acid (LA). On the other hand, cow and goat milk have higher proportions of SFAs (56.2 and 58.02%, respectively) and MUFAs (40.56 and 40.95%, respectively), but lower amounts of PUFAs (3.24 and 1.03%, respectively), with significantly lower quantities of DHA (0.03 and 0.43%, respectively) and LA (1.46 and 2.76%, respectively). Additionally, fatty acids in ruminant milk mainly consist of long-chain fatty acids (LCFAs), with oleic acid being the most abundant. development FA (FA GC. GC . (GC) results variables Additionally NMR (NMR FA. study HM TAGs. TAGs (TAGs) 4921 49 21 49.21 SFAs, , (SFAs) 3432 34 32 34.32 MUFA, MUFA (MUFA) 1647 16 47 16.47 PUFA, PUFA (PUFA) 214 2 14 2.14 (DHA 1302 13 02 13.02 LA. (LA) hand 56.2 562 56 (56. 5802 58 58.02% respectively 40.56 4056 40 (40.5 4095 95 40.95% respectively, 3.24 324 3 24 (3.2 103 1 03 1.03% 0.03 003 0 (0.0 043 43 0.43% 1.46 146 46 (1.4 276 76 2.76% respectively. longchain long chain LCFAs, LCFAs (LCFAs) abundant (GC (TAGs 492 4 49.2 (SFAs 343 34.3 (MUFA 164 16.4 (PUFA 2.1 130 13.0 (LA 56. 5 (56 580 58.02 40.5 405 (40. 409 9 40.95 3.2 (3. 10 1.03 0.0 00 (0. 04 0.43 1.4 (1. 27 7 2.76 (LCFAs 49. 34. 16. 2. 13. (5 58.0 40. (40 40.9 3. (3 1.0 0. (0 0.4 1. (1 2.7 ( 58. (4

This study aims to propose a new method for derivatizing fatty acids (FAs) from human milk (HM), eliminating the lipid extraction step, and simplifying the preparation for gas chromatography with a flame ionization detection (GC-FID) analysis to quantify the FAs. The Design Expert software optimized the reaction times, concentrations, and sample amount. The proposed method (PM) was validated for lyophilized HM and results for the figures of merit for precision in relative standard deviation (RSD) (RSDintra-day 1.34-4.03% and RSDinter day 2.08 5.16%), accuracy (99.87-102.16%), and robustness are within a linear range of 3 to 38% lipids in HM samples. The atmospheric solids analysis probe tandem mass spectrometry (ASAP-MS/MS) technique confirmed the efficiency of PM by expressing the molecular composition of triacylglycerol formed by FAs from the GC-FID technique. The PM requires a small sample size and conducts derivatization directly in the sample matrix, minimizing extraction errors. (FAs HM, , (HM) step GCFID GC FID (GC-FID times concentrations amount (PM RSD (RSD RSDintraday RSDintra 1.344.03% 134403 1.34 4.03% 1 34 4 03 1.34-4.03 208 2 08 2.0 5.16%, 516 5.16% 5 16 5.16%) 99.87102.16%, 998710216 99.87 102.16% 99 87 102 (99.87-102.16%) 38 samples ASAPMS/MS ASAPMSMS ASAP MS/MS MS (ASAP-MS/MS matrix errors (HM 344 1.344.03 13440 134 1.3 403 4.03 0 1.34-4.0 20 2. 51 5.16 87102 99.87102.16% 99871021 9987 99.8 10216 102.16 9 8 10 (99.87-102.16% ASAPMS MSMS 1.344.0 1344 13 1. 40 4.0 1.34-4. 5.1 8710 99.87102.16 9987102 998 99. 1021 102.1 (99.87-102.16 1.344. 4. 1.34-4 5. 871 99.87102.1 998710 102. (99.87-102.1 1.344 1.34- 99.87102. 99871 (99.87-102. 99.87102 (99.87-102 99.8710 (99.87-10 99.871 (99.87-1 (99.87- (99.87 (99.8 (99. (99 (9 (

ABSTRACT Plant growth regulators play an essential role in plants’ biochemical processes. In this study, foliar spray was applied using three growth regulators, gibberellic acid (GA3), naphthalene acetic acid (NAA), and 6-benzyl amino purine (BAP), to investigate their effects on wheat (Triticum aestivum L.) ‘Giza-171’ yield traits and grain biochemical constituents, including sugars, carbohydrates and protein. The assessed growth regulators (GA3, NAA, and BAP) were applied at 100 and 150 mg L-1 concentrations. The results indicated significant improvements in wheat grain yield and 1000-grain weight, which increased by 15.40% and 24.78%, respectively, using GA3 at 150 and 100 mg L-1. In addition, the highest contents of protein and carbohydrates were observed with samples treated by the foliar application of NAA at 150 mg L-1 and BAP at 100 mg L-1, respectively. The fat content in wheat grains increased by 1.97% and 2.73% with foliar spray with 150 mg L-1 NAA and 100 mg L-1 BAP, respectively. The lipid profile showed cis-linoleic acid was the primary fatty acid, followed by palmitic acid; the content of linoleic acid slightly increased with GA3 treatment to 50.42%, while palmitic acid significantly increased with 100 mg L-1 NAA. The total saturated and unsaturated fatty acids recorded 33.64% and 66.30%, respectively; the 150 mg L-1 NAA treatment had the lowest saturated fatty acid content. The SDS-PAGE profiles showed that growth regulators significantly enhanced the wheat protein where new bands appeared, especially in 100 mg L-1 GA3 and 100 mg L-1 BAP treatments. The applied growth regulators improved wheat yield, contents of some compounds, and nutritional value.

Abstract: This study determined the effect of different levels of mamey kernel meal (MKM) as a substitute for ground corn and soybean meal on in vitro gas production, nutrient degradability, fermentation, and protozoan population. The study was carried out in a randomized block design repeated over time. The treatments consisted of seven levels of mamey kernel meal, namely 0, 5, 10, 15, 20, 25, and 30% dry matter (DM), replacing ground corn and soybean meal. The inclusion of MKM had a linear effect on in vitro gas production (P=0.03); the highest production was at 30% inclusion and the lowest at 0%, with 381 and 291 mL g-1, respectively. No differences (P>0.05) were found in gas production, fermentation rate, and lag time between treatments. The maximum volume of gas production (397.43 mL g-1) was at 30%, while the lowest (291.40 mL g-1) was observed at 0% inclusion of MKM. No differences were found in nutrient degradability, protozoan population, and volatile fatty acids (P>0.05). The genera of protozoa that were identified were Entodinium and Holotrich. The different inclusion levels of MKM did not affect the total protozoa population (P>0.05). In conclusion, MKM can be included up to 15% under in vitro conditions without negative effects on fermentative parameters. However, further in vivo studies are needed.