Resumen Beaucarnea hookeri, es una especie endémica de México y está catalogada como amenazada en la NOM-059-SEMARNAT-2010. Con la finalidad de micropropagar esta especie, se estableció un experimento en el Campus Montecillo, Colegio de Posgraduados en 2022, donde se evaluó la respuesta de los brotes durante la multiplicación in vitro con diferentes concentraciones de bencilaminopurina (BAP) y su capacidad de enraizamiento ex vitro durante su aclimatización. Se estableció un experimento con tres subcultivos subsecuentes (SC3, SC4, SC4-1), en SC3 se utilizaron brotes generados con 0.5 mg L-1 de BAP (SC2), en SC4 brotes sanos generados del SC3 y SC4-1 con brotes del SC3, pero, con hiperhidratación inicial (HHI). En el SC3 se evaluaron 0.5, 1, 1.5, 2, 2.5 mg L-1 de BAP. En el SC4 0 y 0.5 mg L-1 BAP y en SC4-1 concentraciones de 0, 0.5 y 1 mg L-1 de BAP. Durante la aclimatización se evaluaron cuatro tratamientos de Radix® 10 000 (0, 1 000, 2 000 y 4 000 mg kg-1) y se utilizaron brotes del SC2. Durante la multiplicación se registró el número de brotes (NB) y el SC3 generó 6.35 brotes con la concentración 1.5 mg L-1 BAP, con presencia de HHI. En el SC4 se obtuvo 9.7 brotes en promedio y en SC4.1, 5.3 brotes, ambos subcultivos con 0.5 mg L-1 de BAP. Además, en el SC4-1 hubo recuperación de HHI. En el enraizamiento no existieron diferencias significativas entre los tratamientos y el testigo a 80 días del trasplante generó 73.3% de brotes con raíz (SBR). Únicamente los brotes del tratamiento testigo en multiplicación presentaron raíces lo que permite obtener plantas completas en 30 días.
Abstract Beaucarnea hookeri is a species endemic to Mexico and is listed as threatened in NOM-059-SEMARNAT-2010. In order to micropropagate this species, an experiment was established at the Montecillo Campus, College of Postgraduates in 2022, where the response of the shoots during in vitro multiplication with different concentrations of benzylaminopurine (BAP) and their capacity for ex-vitro rooting during acclimatization were evaluated. An experiment was established with three subsequent subcultures (SC3, SC4, SC4-1); in SC3, shoots generated with 0.5 mg L-1 BAP (SC2) were used; in SC4, healthy shoots generated from SC3; and SC4-1 with shoots from SC3 but with initial hyperhydration (IHH). In SC3, 0.5, 1, 1.5, 2, and 2.5 mg L-1 BAP were evaluated. In SC4, 0 and 0.5 mg L-1 BAP and in SC4-1, concentrations of 0, 0.5, and 1 mg L-1 BAP. During acclimatization, four treatments of Radix® 10 000 (0, 1 000, 2 000, and 4 000 mg kg-1) were evaluated, and shoots from SC2 were used. During multiplication, the number of shoots (NS) was recorded, and SC3 generated 6.35 shoots with the concentration of 1.5 mg L-1 BAP, with the presence of IHH. On average, 9.7 shoots were obtained in SC4, and 5.3 shoots in SC4-1, both subcultures with 0.5 mg L-1 BAP. In addition, there was a recovery of IHH in SC4-1. In rooting, there were no significant differences between treatments, and the control generated 73.3% of shoots with roots (SSR) 80 days after transplantation. Only the shoots of the control treatment in multiplication presented roots, which allowed complete plants to be obtained in 30 days.