Abstract The SOund Fixing And Ranging (RAFOS) floats were deployed by the Naval Postgraduate School (NPS) near California coast from 1992 to 2004 at depth between 150 and 600 m (http://www.oc.nps.edu/npsRAFOS/). Each drifter trajectory is adaptively decomposed using the empirical mode decomposition (EMD) into low-frequency (non-diffusive, i.e., current) and high-frequency (diffusive, i.e., eddies) components. The identified eddies are mostly anticyclonic with total 203 anticyclonic and 36 cyclonic spirals. Eddy characteristics are determined from the time series of individual RAFOS float trajectory. They are the current velocity scale, eddy radial scale, eddy velocity scale, eddy Rossby number, and eddy-current kinetic energy ratio. The California Current System is found an eddy-rich system with the overall eddy-current kinetic energy ratio of 1.78. It contains submesoscale and mesoscale eddies. The horizontal length scale of 10 km is a good threshold of the eddy radial scale (Leddy) to separate the two kinds of eddies. The mean eddy Rossby number is 0.72 for the submesoscale eddies and 0.06 for the mesoscale eddies. The current-eddy kinetic energy ratio is similar between submesoscale and mesoscale eddies. This may imply similar current-eddy kinetic energy transfer for submesoscale and mesoscale eddies. Statistical characteristics and interannual variability of current velocity scale and eddy characteristic parameters are also presented.

The South-West Indian Ridge in the Indian sector of the Southern Ocean is a region recognised for the creation of particularly intense eddy disturbances in the mean flow of the Antarctic Circumpolar Current. Eddies formed at this ridge have been extensively studied over the past decade using hydrographic, satellite, drifter and float data and it is hypothesised that they could provide a vehicle for localised meridional heat and salt exchange. The effectiveness of this process is dependent on the rate of decay of the eddies. However, in order to investigate eddy decay, logistically difficult hydrographic monitoring is required. This study presents the decay of cold eddies at the South-West Indian Ridge, using outputs from a high-resolution ocean model. The model's representation of the dynamic nature of this region is fully characteristic of observations. On average, 3-4 intense and well-defined cold eddies are generated per year; these eddies have mean longevities of 5.0±2.2 months with average advection speeds of 5±2 km/day. Most simulated eddies reach their peak intensity within 1.5-2.5 months after genesis and have depths of 2000 m - 3000 m. Thereafter they dissipate within approximately 3 months. The decay of eddies is generally characterised by a decrease in their sea surface height signature, a weakening in their rotation rates and a modification in their temperature-salinity characteristics. Subantarctic top predators are suspected to forage preferentially along the edges of eddies. The process of eddy dissipation may thus influence their feeding behaviour.

The ocean currents and their variability south of Africa are known to cover a wide spectrum, spatially and possibly temporally. Hydrographic observations in this vast ocean region are logistically demanding and expensive. In recent years the prevalence of drifting buoys has allowed one to infer certain current characteristics for the region that otherwise would be difficult. Observations from satellite-tracked drifters drogued at a depth of 15 m, collected between 1988 and 2005, were used to infer the mean surface circulation and kinetic energy distributions of the surface flow in the African sector of the Southern Ocean between 30 and 60°S. Regions of intensified flow and of higher levels of eddy kinetic energy were identified and agree fairly well with those established from remote sensing products. These results confirm the value of these observations and indicate the increasing usefulness of this data set as the number of drifter tracks increases.

A linhagem celular Hep-2 é formada por células de carcinoma da laringe e é muito utilizada em modelos de carcinogênese e mutagenêse. Para avaliar o potencial proliferativo desta linhagem, desenvolvemos uma metodologia citogenética (técnica do sobrenadante) para obtenção de metáfases a partir de células que, ao entrarem em mitose, perdem adesão celular, ficando em suspensão no meio de cultura. Através deste procedimento, foram contadas 2000 células, correspondendo a um índice mitótico (IM) de 22.2% . Apesar de o IM obtido por esta técnica não ter sido estatisticamente diferente do IM obtido por outras metodologias citogenéticas clássicas, a técnica do sobrenadante é vantajosa porque elimina o uso de alguns reagentes utilizados na obtenção de metáfases e também diminui o consumo de reagentes de manutenção desta linhagem.

The Hep-2 cell line is derived from laryngeal carcinoma cells and is often utilized as a model in carcinogenesis and mutagenesis tests. To evaluate the proliferative potential of this line, we developed a cytogenetic methodology (drifter technique) to obtain metaphases from cells that loose cellular adhesion when they underwent mitosis in culture. By this procedure, 2000 cells were counted, resulting in a mitotic index (MI) of 22.2%. Although this MI was not statistically different from the one obtained using either a classical cytogenetic method or a cell synchronization technique, the drifter technique has the advantage of not requiring the use of some reagents for the obtention of metaphases and also of diminishing the consumption of maintenance reagents for this cell line.