ABSTRACT: With advancements in coffee cultivation, several traits may be considered in selection of plants, which must exhibit a set of favorable characteristics. The outturn index, defined as the relationship between the mass of mature fruit and processed beans, emerges as a key factor influencing productivity. This study characterized the outturn over two harvests of 57 clones marketed in the public domain and 10 registered cultivars. The analysis considers the effects of genotypes, measurements, and genetic progress achieved through plant selection. According to the maturation cycle of each clone, washed samples of cherry coffee were collected considering a completely randomized factorial design for characterization of the effects of genotypes, years, and the genotype × years (GY) interaction. The drying, peeling, and the outturn index were individually assessed. Despite the significant effects of the GY interaction, genotypes demonstrated minimal alterations in their ranking across measurements. Clones with higher outturn, including LB30, BRS1216, LB12, N7, LB10, LB20, BRS3220, and AS5, exhibited an average outturn of 25.51%, reflecting a gain of 12.17%. Conversely, clones with lower outturn, such as BG180, GJ30, GJ20, AS7, AS10, P42, N1, and P60, had an average of 19.15%, indicating a reduction of 14.02% compared to the general mean. Analyzing the distribution of the outturn values, 19.4%, 62.7%, and 17.9% of genotypes were classified as high, medium, and low outturn, respectively, providing valuable insights for optimal cultivation strategies. ABSTRACT plants characteristics beans productivity 5 1 cultivars measurements clone (GY interaction drying peeling assessed LB30 LB BRS1216 BRS LB12 N7 N LB10 LB20 BRS3220 AS5 AS 2551 25 51 25.51% 1217 12 17 12.17% Conversely BG180 BG GJ30 GJ GJ20 AS7 AS10 P42 P N1 P60 1915 19 15 19.15% 1402 14 02 14.02 mean values 194 4 19.4% 627 62 7 62.7% 179 9 17.9 high medium respectively strategies LB3 BRS121 LB1 LB2 BRS322 255 2 25.51 121 12.17 BG18 GJ3 GJ2 AS1 P4 P6 191 19.15 140 0 14.0 19.4 6 62.7 17. BRS12 BRS32 25.5 12.1 BG1 19.1 14. 19. 62. BRS1 BRS3 25. 12.

RESUMO: Com o avanço da cafeicultura, múltiplas características devem ser consideradas na seleção de plantas, que devem apresentar um conjunto de atributos favoráveis. O rendimento, definido como a relação de massas entre os frutos maduros e os grãos processados, é um importante componente da produtividade. Este estudo tem como objetivo caracterizar o rendimento ao longo de duas colheitas de 57 clones comercializados em domínio público e 10 cultivares registradas. A análise considera efeitos de genótipos, medições e estimativas de ganhos com a seleção. De acordo com o ciclo de maturação de cada clone, amostras de café cereja foram colhidas considerando um delineamento fatorial inteiramente ao acaso para a caracterização dos efeitos de genótipos, anos e interação genótipo × anos (GA). A secagem, o descascamento e o rendimento foram avaliados individualmente. Apesar dos efeitos significativos da interação GA, os genótipos apresentaram pequenas mudanças no ordenamento de uma medição para a outra. Clones com maior rendimento, entre eles, LB30, BRS1216, LB12, N7, LB10, LB20, BRS3220 e AS5 apresentaram rendimento médio de 25,51% associado a estimativas de ganho de 12,17%. Por outro lado, clones de menor rendimento, como BG180, GJ30, GJ20, AS7, AS10, P42, N1 e P60 tiveram uma média de 19,15% associada a uma redução de 14,02%. A partir da distribuição dos valores, 19,4, 62,7 e 17,9% dos genótipos apresentaram rendimento classificados como alto, médio e baixo, subsidiando o melhor cultivo dos genótipos avaliados. RESUMO cafeicultura plantas favoráveis processados produtividade 5 1 registradas clone GA. GA . (GA) secagem individualmente outra eles LB30 LB BRS1216 BRS LB12 N7 N LB10 LB20 BRS322 AS 2551 25 51 25,51 1217 12 17 12,17% lado BG180 BG GJ30 GJ GJ20 AS7 AS10 P42 P P6 1915 19 15 19,15 1402 14 02 14,02% valores 194 4 19,4 627 62 7 62, 179 9 17,9 alto baixo (GA LB3 BRS121 LB1 LB2 BRS32 255 2 25,5 121 12,17 BG18 GJ3 GJ2 AS1 P4 191 19,1 140 0 14,02 19, 6 17, BRS12 BRS3 25, 12,1 BG1 14,0 BRS1 12, 14,

Abstract Understanding forest structure and diversity allows the development and analysis of strategies for the sustainable management of natural resources. The objective of the research was to contribute to the knowledge of tree structure, as well as its regeneration capacity in an altitudinal gradient in a Natural Protected Area in Madera, Chihuahua. The study included three altitudinal intervals: lower (2 307 to 2 466 m), middle (2 466 to 2 625 m) and upper (2 625 - 2 785 m), in which 30 circular sites of 500 m2 were distributed where the diameter and height of the upper trees were measured, as well as the density of regeneration. The vertical structure was obtained with the Pretzsch index (A), in addition, the importance value index (IVI), diversity with the Shannon-Wiener index (H') and richness with Margalef (D mg ) were evaluated, as well as the renewal capacity and damage present. The values of A show the greatest structural homogeneity in interval 2; the Pinaceae family registered the highest ecological weight in the IVI; the values of H' and Dmg were higher in stratum II, however, all are considered of low diversity and richness; the species Abies concolor and Pseudotsuga menziesii, showed the highest proportion of regeneration in the community, in which suppression is the frequent damage, however, 95% of the individuals were not affected. This information is useful for the conservation of these ecologically important communities in temperate forests.

Resumen Comprender la estructura y diversidad forestal permite elaborar y analizar estrategias para el manejo sustentable de los recursos naturales. El objetivo de la investigación fue contribuir al conocimiento de la estructura arbórea, así como su capacidad de regeneración en un gradiente altitudinal en un Área Natural Protegida, en Madera, Chihuahua. El estudio comprendió tres intervalos altitudinales: inferior (2 307 a 2 466 m), medio (2 466 a 2 625 m) y superior (2 625 - 2 785 m), en los que se distribuyeron 30 sitios circulares de 500 m2 donde se midió el diámetro y altura del arbolado superior, así como la densidad de la regeneración. La estructura vertical se obtuvo con el índice de Pretzsch (A), además, se evaluó el índice de valor de importancia (IVI), la diversidad con el índice de Shannon-Wiener (H’) y riqueza con Margalef (D mg ), asimismo, se estimó la capacidad de renuevo y los daños presentes. Los valores de A muestran la mayor homogeneidad estructural en el intervalo 2; la familia Pinaceae registró el peso ecológico superior en el IVI; los valores de H’ y D mg fueron superiores en el estrato II, sin embargo, todos se consideran de baja diversidad y riqueza; las especies Abies concolor y Pseudotsuga menziesii, mostraron la mayor proporción de regeneración en la comunidad, en la que la supresión es el daño frecuente, no obstante, el 95% de los individuos no presentaron afectaciones. Esta información es de utilidad para la conservación de estas comunidades de importancia ecológica en bosques templados.

Abstract The objective of this study was to describe the diversity, composition, and structure of the woody vegetation in a section of LDF in Lerma, Campeche. Between August and September 2018, 10 plots of 10 × 10 m2 (1 000 m2 in total) were delimited, measuring all woody individuals with diameter at breast height (DBH = 1.30 cm). The structure of the site was described based on the relative values of density, frequency, and dominance (IVI), as well as the distribution of diameter classes. Diversity was estimated with the Shannon-Wiener index (H'), while equity was measured by the Pielou index. 40 woody species were recorded, belonging to 35 genera and 19 families. The H' index had a value of 2.92, and the Pielou index was 0.79, the density was 8 950 ind ha-1. Gymnopodium floribundum (204 individuals) and Lonchocarpus xuul (129) were the most abundant species. Bursera simaruba and Lysiloma latisiliquum showed higher diameter values (36 and 28.6 cm DBH, respectively). The families Rubiaceae (eight species), Fabaceae (five species), Ebenaceae and Polygonaceae (three species each) were the most representative. The species with the highest IVI were Gymnopodium floribundum (48.24%), Lonchocarpus xuul (29.12%), Bursera simaruba (26.11%) and Lysiloma latisiliquum (24.98%), these species being the most important in the structure of the LDF in the site. The distribution of the diameter classes suggests that the section of the LDF in Lerma Campeche is in an adequate state of regeneration.

Resumen El objetivo de este estudio fue describir la diversidad, composición y estructura de la vegetación leñosa en una sección de la selva baja caducifolia (SBC) en Lerma, Campeche. Entre agosto y septiembre 2018, se delimitaron 10 parcelas de 10 × 10 m2 (1 000 m2 en total) midiendo todos los individuos de 1 cm de DAP (diámetro a la altura del pecho, 1.30 m). Se describió la estructura del sitio basado en los valores relativos de densidad, frecuencia y dominancia (IVI), así como la distribución de clases diamétricas. La diversidad se estimó con el índice de Shannon-Wiener (H’), mientras que la equidad fue medida por el índice de Pielou. Se registraron 40 especies leñosas, pertenecientes a 35 géneros y 19 familias. El índice H’ tuvo un valor de 2.92, y el índice de Pielou fue de 0.79, la densidad fue de 8 950 ind ha-1. Gymnopodium floribundum (204 individuos) y Lonchocarpus xuul (129 individuos) fueron las especies más abundantes. Bursera simaruba y Lysiloma latisiliquum mostraron valores diamétricos más altos (36 y 28.6 cm de DAP, respectivamente). Las familias Rubiaceae (ocho especies) y Fabaceae (cinco especies), fueron las más representativas. Las especies con mayor IVI fueron Gymnopodium floribundum (48.24%), Lonchocarpus xuul (29.12%), Bursera simaruba (26.11%) y Lysiloma latisiliquum (24.98%), siendo estas especies las más importantes en la estructura de la SBC en el sitio. La distribución de las clases diamétricas sugiere que la sección de la SBC en Lerma Campeche se encuentra en un adecuado estado de regeneración.

The South African food system is facing severe challenges as increasing hunger, rising food costs, lack of dietary diversity, child stunting, foodborne illnesses, food waste and an obesity epidemic coupled with malnutrition are observed. In this study, we aimed to establish the application of science and technology advances in the food and beverage industry in South Africa in response to food consumption pattern changes since 1994 and how they could be used to address food security challenges. We found that food consumption shifts have been towards sugar-sweetened beverages, processed and packaged food, animal-source foods and added caloric sweeteners, and away from vegetables. These dietary shifts are concerning as they relate to public health. Most commitments to improve the nutritional status of South Africans have been limited to corporate social investment strategies and should be extended into core business strategies. Furthermore, although the South African food and beverage industry has kept pace with developments in food manufacturing practices, there has been little experimentation with non-commercial novel technologies. The expert survey revealed that indigenous African crops and food waste recovery are the two most promising emerging food sources that could be available to South Africans in the shortest time frame. South Africa has many enabling drivers to become a global leader in food technology advances. However, many barriers need to be overcome for industry, academia and government to collaborate to advance novel food science and technologies to reach commercialisation. SIGNIFICANCE: • Drivers and consequences of food consumption changes in South Africa were modelled and broad food consumption trends between 1994 and 2009/2012 were identified. • For the first time, the Access to Nutrition Index methodology was applied to South African owned food companies to identify strategies to enhance nutrition practices. Companies need to do more to deliver affordable and accessible products. • Drivers and barriers in adopting advanced food science and technology were modelled. A collective ecosystem approach with industry, academia and government mobilisation around critical areas like hunger, malnutrition and poverty could be a way to tackle the failing food system.

Resumen La vegetación es una parte vital de la infraestructura urbana por los servicios ambientales y los beneficios que provee a la población, por lo que es importante conocer su diversidad y estructura. El objetivo del presente trabajo fue evaluar la estructura y composición florística de árboles, palmas y arbustos ubicados en áreas urbanas públicas de la ciudad de Tapachula, en el estado de Chiapas. Se recabó información dasométrica de ocho parques públicos, cinco áreas verdes públicas, cuatro camellones o jardineras y 38 calles, con una superficie total de 27.32 ha. Se registró el diámetro normal (1.30 m de la base del suelo) y la altura total. Se calculó la densidad de especies por área verde. La diversidad fue evaluada mediante los índices de Shannon-Weiner y de Margalef. Taxonómicamente, se identificaron 130 especies, 109 géneros y 44 familias. De los taxa registrados, 65 pertenecen a especies nativas (550 individuos) y 65 a exóticas (1 927 individuos). Ficus microcarpa es el taxón exótico preponderante con un Índice de Valor de Importancia (IVI) de 6.14. De las especies nativas, Roseodendron donnell-smithii destaca como el elemento más representativo (IVI=1.45). El Índice de Shannon-Weiner calculado para diferentes áreas verdes registró 2.7≤H’≤3.3 y el Índice de Margalef 5.2≤D Mg ≤15.2, lo cual evidencia que las calles poseen una gran diversidad de especies.

Abstract Urban vegetation is a vital part of urban infrastructure due to the environmental services it provides and the benefits to the population; therefore, it is important to know its diversity and structure. The objective of this work was to assess the structure and floristic composition of trees, palms and shrubs located in public urban areas of the city of Tapachula, state of Chiapas. Mensuration information was collected from eight public parks; five public green areas, four medians and 38 streets, with a total area of 27.32 ha. Normal diameter (1.30 m base from the ground) and total height were recorded. The density of species per urban green area was calculated. Diversity was evaluated using the Shannon-Weiner and Margalef indexes. Taxonomically, 130 species, 109 genera and 44 families were identified. Of the 130 specific taxa recorded, 65 belong to native species and 65 exotic. The total number of individuals was 1 927 exotic and 550 native. Ficus microcarpa is the predominant exotic with 6.14 Importance Value Index (IVI). Of the native species, Roseodendron donnell-smithii stands out as the most representative (IVI=1.45). The Shannon-Weiner Index calculated for different green areas registered 2.7≤H'≤3.3 and the Margalef Index 5.2≤D Mg ≤15.2, showing that the streets have a great species diversity.

Resumen Los cafetales (Coffea arabica) de la región de la Montaña de Guerrero mantienen las características de un bosque, además contribuyen a la generación de recursos económicos que ayudan a un gran número de familias a mitigar parte de sus necesidades básicas. El objetivo de este trabajo fue identificar la estructura y los usos de las especies de sombra de los cafetales en dos comunidades Me’phaa de la Montaña de Guerrero. Se muestrearon 12 parcelas en Tres Cruces (TC), Acatepec y La Ciénega (LC), Malinaltepec; se utilizó el método de punto-centro-cuadrado; a los árboles se les midió la distancia, altura, cobertura de copa y diámetro normal. Se estimó la diversidad alfa basada en la riqueza de especies (S), el Índice de Simpson, Shannon-Wiener y el número efectivo de especies (N0, N1 y N2), la similitud se calculó con el Coeficiente Jaccard (I J ) y se determinó el Índice de Valor de Importancia (IVI). Para reconocer los usos de los árboles se aplicaron entrevistas (50 en TC y 10 en LC) estructuradas a los cafeticultores. Se registraron 35 especies, 16 en Tres Cruces y 22 en La Ciénega, de las cuales 83.3 % fueron nativas, por lo que se clasifica como manejo tradicional. Alnus acuminata en LC y Myrsine juergensenii en TC fueron las de mayor IVI. Los cafeticultores señalaron a 24 especies en TC y 14 en LC que proporcionan sombra, generan materia orgánica, frutos, flores y combustibles; las especies más empleadas son Clethra mexicana y A. acuminata.

Abstract The coffee (Coffea arabica) plantations of the Montaña de Guerrero region preserve the characteristics of a forest and also contribute to the generation of economic resources that help a large number of families to meet some of their basic needs. The objective of this work was to identify the structure and uses of shade species in coffee plantations in two Me'phaa communities in the Montaña de Guerrero region. Twelve plots in Tres Cruces (TC), Acatepec and La Ciénega (LC), Malinaltepec, were sampled using the point-center-square method; trees were measured for distance, height, crown cover and normal diameter. The alpha diversity was estimated based on species richness (S), Simpson's Index, Shannon-Wiener’s Index, and the effective number of species (N0, N1 and N2); similarity was calculated with the Jaccard index (J I ), and the Importance Value Index (IVI) was determined. In order to identify the uses of the trees, structured interviews (50 in TC and 10 in LC) were carried out with coffee growers. Thirty-five species were recorded -16 in Tres Cruces and 22 in La Ciénega-, of which 83.3 % were native; therefore, the region is classified as under traditional management. Alnus acuminata in LC and Myrsine juergensenii in TC had the highest IVI. Coffee growers reported 24 species in TC and 14 in LC that provide shade, generate organic matter, fruits, flowers, and fuel. The most commonly used species are Clethra mexicana and A. acuminata.

Abstract: The dry forest ecosystem is characterized by their rich biodiversity and adaptations to arid conditions. This study focused on determining the composition and structure of the vegetation, examining species interactions, and estimating carbon stored in its aboveground biomass (AGB) using an allometric equation proposed for mixed dry forests. We used 10 plots of 10 x 20 m to record data on trees with a diameter at breast height (DBH) ≥ 5 cm. Taxonomic classification was initially obtained using experts and specialized databases. Ecological importance was assessed through the Importance Value Index (IVI), and species association was determined using the Indicator Value Index (IndVal%). We identified 148 individuals of 21 species, 19 genera, and 12 families in four groups with strong associations, with C. Trischistandra standing out for its high IVI. The Kruskal-Wallis test did not show significant differences in carbon stored between plots, and was estimated a storage potential of 70.47 Mg C ha-1. This research highlights the importance of key species in carbon capture, which is crucial for mitigating climate change. Effective management of these species could have a positive impact on the conservation of the dry forest ecosystem and the fight against global warming. This analysis provides a deep understanding of the structure of this ecosystem.

Resumen: El ecosistema del bosque seco se caracteriza por su rica biodiversidad y adaptaciones a condiciones áridas. Este estudio se centró en determinar la composición y estructura de la vegetación, examinar las interacciones entre las especies y estimar el carbono almacenado en su biomasa aérea (AGB), se usó una ecuación alométrica propuesta para bosques secos mixtos. Utilizamos 10 parcelas de 10 x 20 m para registrar datos sobre árboles con un diámetro a la altura del pecho (DAP) ≥ 5 cm. Inicialmente, la clasificación taxonómica se obtuvo consultando a expertos y bases de datos especializadas. La importancia ecológica se evaluó a través del Índice de Valor de Importancia (IVI) y la asociación de especies se determinó utilizando el Índice de Valor Indicador (IndVal%). Se identificaron 148 individuos de 21 especies, 19 géneros y 12 familias en cuatro grupos con fuertes asociaciones, destacándose C. Trischistandra por su alto IVI. El test de Kruskal-Wallis no mostró diferencias significativas en el carbono almacenado entre las parcelas y se estimó un potencial de almacenamiento de 70.47 Mg C ha-1. Esta investigación resalta la importancia de especies clave en la captura de carbono, lo cual es crucial para mitigar el cambio climático. El manejo efectivo de estas especies podría tener un impacto positivo en la conservación del ecosistema de bosque seco y en la lucha contra el calentamiento global. Este análisis brinda un entendimiento profundo de la estructura de este ecosistema.

Abstract Background: One of the most diverse and threatened plant associations in the Yucatan peninsula has not been characterized and therefore not included in any protection category in Mexico. We characterize and describe this plant association, which is rapidly disappearing or being irreversibly transformed as a result of anthropic activities. We propose a name and attempt a preliminary assessment of its taxonomic richness, and the identification of priority species to be preserved. Questions: What is the species richness and plant endemism associated within this plant association? What are the ecologically important and high-priority species for conservation? How is it different from associated or similar plant associations? Studied species: Vascular plants. Study site and dates: Northern Yucatan peninsula; 2021-2023. Methods: The plant association was characterized, and quantitative parameters were recorded. The Importance Value Index was estimated to assess its local ecological importance, and each species was assigned a conservation category in order to evaluate the conservation status in a global context. Results: We propose Seasonally flooded Coquinal (SFC) as a name for this plant association based on geomorphological, physiognomic, and structural attributes. A total of 206 species were recorded, two of which are endemic to the SFC and 28 to the Yucatan Peninsula Biotic Province (YPBP). Twelve species are listed under a risk category. Conclusions: The SFC harbors a high plant diversity of species endemic species to the YPBP, 12 of which are included in the IUCN red list, for which its typification and conservation should be a high priority in Mexico.

Resumen Antecedentes: Una de las asociaciones vegetales más diversas y amenazadas de la península de Yucatán no ha sido reconocida, por tanto, no incluida en alguna categoría de protección en México. Se caracterizó y describió esta vegetación, la cual está desapareciendo o siendo transformada resultado de actividades antrópicas. Proponemos un nombre y realizamos una primera aproximación al conocimiento de su riqueza taxonómica y especies prioritarias a conservar. Preguntas: ¿Cuántas especies de plantas hay y cuáles son los endemismos que contiene? ¿Cuáles son las especies con mayor importancia ecológica y prioritarias para conservar? ¿Cómo se puede diferenciar de vegetación asociada o similar? Especies estudiadas: Plantas vasculares. Sitio y fechas de estudio: Norte de la Península de Yucatán; 2021-2023. Métodos: Se caracterizó la asociación vegetal y se registraron parámetros cuantitativos. El Índice de Valor de Importancia fue estimado para evaluar la importancia ecológica local y la categoría de conservación de las especies fue asignada evaluando el estatus de conservación en un contexto global. Resultados: En función de sus características geomorfológicas, fisonómicas y estructurales, proponemos “Coquinal Estacionalmente Inundado” (CY) como un nombre para esta asociación vegetal. Un total de 206 especies fueron registradas, incluyendo dos especies endémicas al CY y 28 endémicas a la Provincia Biótica Península de Yucatán (PBPY). Doce especies están en alguna categoría de riesgo. Conclusiones: El CY alberga una alta diversidad de especies endémicas a la PBPY, 12 de ellas incluidas en la lista roja de la IUCN, por lo que su tipificación y conservación es de alta prioridad en México.

Abstract Rice (Oryza sativa L.) is an important source of energy in the human diet; and, in Nicaragua, it constitutes a substantial component of the population's diet. The present study was exploratory, and aimed to determine the diversity of arthropods associated with rice cultivation in the municipality of Sébaco, an intensive rice production region, in the 2022-2023 agricultural cycle. Production systems with extensions over 100 hectares, where equal agronomic and phytosanitary management was used, were selected. For entomofauna monitoring, ten points were randomly selected in each production system, and at each point, and 20 double zigzag passes with entomological nets were used at each point. The number of individuals per order and family was quantified. For the diversity analysis, they were grouped according to their feeding habits. Abundance and richness information was analyzed using descriptive analysis, analysis of variance, and Shannon Weaver and Simpson's diversity index. Seven orders, 13 families, and 16 genera were found, most of them of the phytophagous habit. The highest abundance corresponded to the species T. orizicolus Muir (n = 46,663), reported in the reproductive and maturation phases. Likewise, Hydrellia sp (n = 5,229) presented the largest populations in the vegetative phase. Among the beneficial organisms, siders (n = 1,523) and Atrichopogum sp (n = 419) stood out. Diversity indices were low both in the production systems and in the different phenological phases of the crop.

Resumen El cultivo de arroz (Oryza sativa L.) representa una fuente de energía de importancia en la dieta humana, y en Nicaragua constituye un sustancial componente de la dieta de la población. El presente estudio fue exploratorio y tuvo como objetivo determinar la diversidad de artrópodos asociada al cultivo de arroz en el municipio de Sébaco, en el ciclo agrícola 2022-2023, una región intensiva en la producción de arroz. Se seleccionaron sistemas productivos con extensiones mayores a 100 hectáreas, en donde se empleó igual manejo agronómico y fitosanitario. Para el monitoreo de la entomofauna, en cada sistema productivo se seleccionaron diez puntos al azar y en cada punto se emplearon 20 pases dobles en forma de zig-zag con red entomológica. Se cuantifico el número de individuos por orden y familia; para el análisis de diversidad se agruparon por sus hábitos alimenticios. La información de la abundancia y riqueza se analizó mediante estudio descriptivo, análisis de varianza e índice de diversidad de Shannon Weaver y Simpson. Se encontraron siete órdenes, 13 familias y 16 géneros, la mayoría del hábito fitófago. La mayor abundancia correspondió a la especie T. orizicolus Muir (n=46.663), reportada en la fase reproductiva y maduración. Así mismo, Hydrellia sp (n=5.229), presentó las mayores poblaciones en la fase vegetativa. Dentro de los organismos benéficos sobresalieron las arañas (n=1.523) y Atrichopogum sp (n=419). Los índices de diversidad fueron bajos tanto en los sistemas productivos como en las diferentes fases fenológicas del cultivo.

Insects are one of the most species-rich groups on Earth. They comprise much of animal diversity and play vital roles in ecosystems, including pollination, pest control, and decomposition. However, only a fraction of this diversity has been formally described. South Africa is recognised as one of the most biologically diverse countries globally, with an estimated 44 000 insect species. Many crops rely on insect pollinators, including canola, apples, oranges, and sunflowers. A shortage of wild pollinators currently threatens crop yields, yet our knowledge of insect diversity within South Africa is sparse. There are few taxonomic specialists relative to South Africa's biodiversity, and the methods used for insect identification can be time-consuming and expensive. DNA barcoding provides an important research tool to accelerate insect biodiversity research. In this review, we queried the public DNA barcoding BOLD (Barcode of Life Data System) database for records of "Insecta" within South Africa, and 416 211 published records assigned to 28 239 unique BINs (Barcode Index Numbers) were returned. We identified five taxonomic orders with more BINs than known species in southern Africa (Hymenoptera, Diptera, Thysanoptera, Plecoptera, and Strepsiptera). Most of the barcoded records were derived from Malaise trap sampling in Gauteng, Mpumalanga and Limpopo, while the rest of South Africa remains poorly sampled. We suggest that there is a need for a comprehensive national sampling effort alongside increased investment in taxonomic expertise to generate critical baseline data on insect biodiversity before species are lost to extinction. SIGNIFICANCE: Insects provide critical ecosystem services, but our knowledge of insect biodiversity is limited. DNA barcoding can help fill biodiversity knowledge gaps; however, within South Africa, sampling has been uneven. Well-sampled provinces include Gauteng, Mpumalanga and Limpopo, while the Eastern Cape, Free State, KwaZulu-Natal, Northern Cape, North-West and Western Cape remain under-sampled. We argue for a broad national Malaise trapping effort to generate crucial baseline data on insect biodiversity. Without urgent investment in taxonomic expertise and biomonitoring, we risk losing much of our biodiversity before it is even described.

ABSTRACT Lessonia nigrescens and L. trabeculata are economically important kelp species in northern Chile. Despite their considerable value, the genetic diversity of these kelp has received limited attention in the Atacama region. This study aimed to analyze genetic diversity, establish genetic relationships, and identify diagnostic DNA markers in the Atacama region's L. nigrescens and L. trabeculata. Both species' Inter-sequence simple repeat (ISSR) band patterns showed high reproducibility and reliability. Among 17 ISSRs, 161 polymorphic bands were identified in Lessonia spp. Parameters, such as percentage polymorphism, number of alleles per locus, effective number of alleles, and Shannon information index, indicated that L. nigrescens had slightly higher genetic diversity than L. trabeculata. The Unweighted pair group method with arithmetic average (UPGMA) cluster analysis revealed two main clusters: one containing L. nigrescens and the other containing L. trabeculata. Additionally, four species diagnostic ISSR markers were identified for L. nigrescens and L. trabeculata. The ISSR markers proved valuable in detecting genetic diversity in L. nigrescens and L. trabeculata. This information is particularly important for marine management strategies and ensuring successful conservation.

ABSTRACT The study of the structure and diversity of forest ecosystems is essential to understand the functioning and dynamics of forests. The structure and diversity of woody species in different types of watercourses along the Santa Catarina river in Nuevo Leon, Mexico were described. Three conditions were identified: permanent (I), semi-permanent (II) and temporary (III). In each condition, 14 rectangular sites of 1 000 m2 were established. All species were identified. The crown diameter of each individual was measured. The Importance Value Index (IVI) was determined for each condition. Species richness and diversity was calculated with the Margalef (D Mg ) and Shannon-Wiener (H') indices. In addition, the similarity between conditions was determined using the Sorensen index (Is). 32 species, 29 genus and 16 families were identified. Condition I recorded greater specific richness and greater diversity with 28 species and a diversity value H' = 2.41 ± 0.710, where Salix nigra presented the highest IVI. Condition II presented the highest Margalef richness with a D Mg value = 1.77 ± 0.22. In condition III Leucaena leucocephala turned out to be the most important species. The similarity between conditions was greater than 50%. The results demonstrate the structural variation of the vegetation in different hydrological regimes, which constitutes a solid base for future investigations on the development of the vegetation associated with the Santa Catarina river, and other riparian areas of north of Mexico.

RESUMEN El estudio de la estructura y diversidad de los ecosistemas forestales es fundamental para comprender el funcionamiento y dinámica de los bosques. Se describió la estructura y diversidad de especies leñosas en diferentes tipos de curso de agua a lo largo del río Santa Catarina en Nuevo León, México. Se identificaron tres condiciones hidrológicas: permanente (I), semipermanente (II) y temporal (III). En cada condición se establecieron 14 sitios rectangulares de 1 000 m2, todas las especies de cada sitio fueron identificadas. Se midió el diámetro de la copa de cada individuo, el Índice de Valor de Importancia (IVI) de condición. La riqueza y diversidad de especies fue calculada con los índices de Margalef (D Mg ) y Shannon-Wiener (H'). Además, se determinó la similitud entre condiciones con el índice de Sorensen (Is). Se identificaron 32 especies, 29 géneros y 16 familias. La condición I registró mayor riqueza específica y diversidad con 28 especies y un valor de diversidad H' = 2.41 ± 0.710, en donde Salix nigra presentó el mayor IVI. La condición II presentó la mayor riqueza de Margalef con un valor D Mg de 1.77 ± 0.22. En la condición III Leucaena leucocephala fue la especie más importante. La similitud entre condiciones fue mayor al 50%. Los resultados demuestran la variación estructural de la vegetación en diferentes regímenes hidrológicos, lo cual constituye una base sólida para futuras investigaciones sobre el desarrollo de la vegetación asociada al río Santa Catarina, y otras áreas riparias del norte de México.

ABSTRACT Understanding the dietary needs is important for the conservation of the bighorn sheep. The objective was to identify the diet selection in Coahuila, Mexico. The research was conducted at the Rancho San Juan Wildlife Management Unit (UMA), Monclova municipality, from August 2018 to October 2019. Forage availability was assessed using 18 Canfield transects. Diet composition was estimated through microhistology on 280 fecal samples, and diet and forage diversity were assessed using the Shannon index. Cluster and principal component analyses (PCA) were applied to analyze similarity in consumption across different species and seasonal influences. Shrubs were the most available (48%). A total of 50 species and 14 families were identified in the diet, with shrubs (49.2%) and grasses (17.21%) being predominant. Tiquilia canescens, Gymnosperma glutinosum, Opuntia rufida, Medicago sativa, and Erioneuron pulchellum were the most consumed species. The bighorn sheep consumed tree and shrub species proportionally (1.1 and 1.02, respectively), preferred herbaceous plants in spring (2.24) and autumn (15.28), and grasses in spring (6.10), except in summer (0.83) when they avoided succulents. PCA revealed that the first two components explained 96.05% of the correlation between species consumption frequencies and their biological forms. Cluster analysis grouped five forage consumption categories. Conserving shrub species, which form the basis of the bighorn sheep's diet, grasses-its preference-and succulents as buffering elements, is essential.

RESUMEN Comprender las preferencias alimentarias es importante para la conservación del borrego cimarrón. El objetivo fue identificar la selección de su dieta en Coahuila, México. La investigación se realizó en la UMA Rancho San Juan, municipio de Monclova, de agosto 2018 a octubre 2019. La disponibilidad de forraje se evaluó con 18 líneas de Canfield. La composición de la dieta se estimó con microhistología en 280 muestras fecales, y la diversidad de la dieta y del forraje con el índice de Shannon. Para analizar la similitud en el consumo de diferentes especies y la influencia estacional, se aplicaron análisis de clústers y componentes principales (ACP). Las arbustivas estuvieron más disponibles (48%). Se identificaron 50 especies y 14 familias en la dieta, y predominaron las arbustivas (49.2%) y los pastos (17.21%). Tiquilia canescens, Gymnosperma glutinosum, Opuntia rufida, Medicago sativa y Erioneuron pulchellum fueron las más consumidas. El borrego consumió especies arbóreas y arbustivas de manera proporcional (1.1 y 1.02, respectivamente), prefirió las herbáceas en primavera (2.24) y otoño (15.28), los pastos en primavera (6.10), y con excepción de verano (0.83), evitó las suculentas. El ACP reveló que los dos primeros componentes explican el 96.05% de la correlación entre las frecuencias de consumo de las especies y sus formas biológicas. El análisis de clústers agrupó cinco categorías de consumo de forraje. Es fundamental conservar las arbustivas, que constituyen la base de la dieta del borrego cimarrón, los pastos, que son sus preferidos, y las suculentas, como elementos amortiguadores.

Abstract The ecotone is of great importance given the environmental services they provide both to the environment and to human wellbeing. The objective was to know the structure and floristic composition of a contact zone between two types of bushes and also to know the potential it has as a carbon sink. Importance value indices, vertical species distribution, Margalef, Shannon, and true Shannon diversity, as well as biomass, total area, and carbon concentration were determined. Eleven species of vascular plants were recorded, the best represented family was Fabaceae and Cordia boisieri was the species with the highest importance value index. The diversity of the plant community is low according to the Shannon index. The total canopy area was 4 276.03 m2 ha-1, while the vertical index of species defined three height strata. The stored carbon is 2.82 Mg ha-1, while the total aerial biomass was 5 667.15 kg ha-1.

Resumen El ecotono es de gran importancia dado los servicios ambientales que prestan tanto al ambiente como al ser humano. El objetivo fue conocer la estructura y la composición florística en una zona de contacto entre dos tipos de matorrales y conocer además el potencial que tiene como sumidero de carbono. Se determinaron los índices de valor de importancia, distribución vertical de especies, Margalef, Shannon y la diversidad verdadera de Shannon. Además de la biomasa, área total y la concentración de carbono. Se registraron 11 especies de plantas vasculares, la familia mejor representada fue Fabaceae. La especie Cordia boisieri tuvo el mayor índice de valor de importancia. La diversidad de la comunidad vegetal fue baja. El área total de copa fue de 4 276.03 m2 ha-1, con tres estratos de altura. El carbono almacenado fue de 2.82 Mg ha-1, mientras que la biomasa aérea total fue de 567.15 kg ha-1.

RESUMEN Es conocido que los bosques, según su estado de conservación, influyen en la calidad ecológica de la red hídrica, en especial las aguas para mantenimiento de la humedad del suelo, si éstas poseen calidad, se atenúan los efectos de la sequía, favoreciendo también la calidad de los suelos; el servicio ecosistémico de biodiversidad con la presencia de un adecuado índice de diversidad de especies, tanto de la flora como de la fauna, garantiza los procesos necesarios relacionados con la polinización, el control natural de plagas, y la calidad estética del paisaje. Para ello, se evaluó el papel de bosques y algunos de sus servicios ecosistémicos, dentro de un polígono demostrativo, para presentar a una propuesta de medidas que favorezcan los mismos, identificándose indicadores y variables de cada servicio ecosistémico estudiado, teniendo en cuenta las problemáticas de cada uno; permitiendo la operacionalización de las variables durante la evaluación, con el fin de mejorar el estado del agua, los suelos y la biodiversidad en el polígono demostrativo Los Monsegui.

RESUMO Sabe-se que as florestas, dependendo de seu estado de conservação, influenciam a qualidade ecológica da rede hídrica, especialmente a água utilizada para manter a umidade do solo; se forem de boa qualidade, os efeitos da seca são atenuados, favorecendo também a qualidade do solo; o serviço ecossistêmico da biodiversidade, com a presença de um índice adequado de diversidade de espécies, tanto da flora quanto da fauna, garante os processos necessários relacionados à polinização, ao controle natural de pragas e à qualidade estética da paisagem. Para isso, o papel das florestas e alguns de seus serviços ecossistêmicos foram avaliados em um polígono de demonstração, a fim de apresentar uma proposta de medidas que os favoreçam, identificando indicadores e variáveis para cada serviço ecossistêmico estudado, levando em conta os problemas de cada um deles; permitindo a operacionalização das variáveis durante a avaliação, com o objetivo de melhorar o estado da água, dos solos e da biodiversidade no polígono de demonstração de Los Monsegui.

ABSTRACT It is known that forests, depending on their state of conservation, influence the ecological quality of the water network, especially the waters to maintain soil humidity. If these have quality, the effects of drought are attenuated, also favoring the soil quality. The ecosystem service of biodiversity with the presence of an adequate index of species diversity, both flora and fauna, guarantees the necessary processes related to pollination, natural pest control, and the aesthetic quality of the landscape. For this, the role of forests and some of their ecosystem services were evaluated, within a demonstrative polygon, to present a proposal for measures that favor them, identifying indicators and variables of each ecosystem service studied, taking into account the problems of each one; allowing the operationalization of the variables during the evaluation, in order to improve the state of water, soils and biodiversity in the Los Monsegui demonstration site.