ABSTRACT Purpose: The epithelial–mesenchymal transition of human lens epithelial cells plays a role in posterior capsule opacification, a fibrotic process that leads to a common type of cataract. Hyaluronic acid has been implicated in this fibrosis. Studies have investigated the role of transforming growth factor (TGF)-β2 in epithelial–mesenchymal transition. However, the role of TGF-β2 in hyaluronic acid-mediated fibrosis of lens epithelial cell remains unknown. We here examined the role of TGF-β2 in the hyaluronic acid-mediated epithelial–mesenchymal transition of lens epithelial cells. Methods: Cultured human lens epithelial cells (HLEB3) were infected with CD44-siRNA by using the Lipofectamine 3000 transfection reagent. The CCK-8 kit was used to measure cell viability, and the scratch assay was used to determine cell migration. Cell oxidative stress was analyzed in a dichloro-dihydro-fluorescein diacetate assay and by using a flow cytometer. The TGF-β2 level in HLEB3 cells was examined through immunohistochemical staining. The TGF-β2 protein level was determined through western blotting. mRNA expression levels were determined through quantitative real-time polymerase chain reaction. Results: Treatment with hyaluronic acid (1.0 μM, 24 h) increased the epithelial–mesenchymal transition of HLEB3 cells. The increase in TGF-β2 levels corresponded to an increase in CD44 levels in the culture medium. However, blocking the CD44 function significantly reduced the TGF-β2-mediated epithelial–mesenchymal transition response of HLEB3 cells. Conclusions: Our study showed that both CD44 and TGF-β2 are critical contributors to the hyaluronic acid-mediated epithelial-mesenchymal transition of lens epithelial cells, and that TGF-β2 in epithelial-mesenchymal transition is regulated by CD44. These results suggest that CD44 could be used as a target for preventing hyaluronic acid-induced posterior capsule opacification. Our findings suggest that CD44/TGF-β2 is crucial for the hyaluronic acid-induced epithelial-mesenchymal transition of lens epithelial cells. Purpose epithelialmesenchymal mesenchymal opacification cataract TGFβ2 TGFβ TGF β2 β (TGF)-β However TGF-β acidmediated mediated unknown Methods HLEB (HLEB3 CD44siRNA CDsiRNA siRNA CD 300 reagent CCK8 CCK 8 CCK- viability migration dichlorodihydrofluorescein dichloro dihydro fluorescein cytometer staining blotting realtime real time reaction Results 1.0 10 1 0 (1. μM 2 h CD4 medium TGFβ2mediated TGFβmediated Conclusions acidinduced induced CD44/TGFβ2 CD44TGFβ2 CDTGFβ CD44/TGF CD44/TGF-β (HLEB 30 1. (1 CD44/TGFβ CD44TGFβ CD44TGF CDTGF 3 (

RESUMO Kalanchoe daigremontiana é uma espécie ornamental de propagação obrigatoriamente assexuada. Entretanto, nenhum estudo in vitro foi realizado para avaliar a relação entre mudanças no ambiente de cultivo e variações genéticas de K. daigremontiana. Portanto, o objetivo deste estudo foi estabelecer a melhor concentração de sais no meio Murashige & Skoog (MS) e a concentração de sacarose no meio de cultura, além da qualidade da luz, no desenvolvimento de plântulas de explantes foliares de K. daigremontiana. Além disso, realizamos uma avaliação genética das plântulas resultantes para estudar alterações fenotípicas que poderiam ser atribuídas à variação somaclonal utilizando marcadores da Amplificação Aleatória de DNA polimórfico (RAPD). Para tanto, explantes foliares de K. daigremontiana foram desinfestados e inoculados em diferentes concentrações de sais do meio MS (25, 50 ou 100%), sem sacarose. As culturas foram transferidas para sala de crescimento e submetidas a três condições de luz (branca, azul e vermelha) com fotoperíodo de 16 horas, mais a ausência de luz, sob temperatura de 27 ± 1 °C. Os explantes também foram inoculados em 25% das concentrações de sais do meio MS, suplementado com diferentes concentrações de sacarose (controle - sem sacarose; 1,5; 3,0; 4,5 e 6,0%). Os explantes foliares de K. daigremontiana desenvolveram-se melhor sob luz branca e em qualquer uma das concentrações de sal do meio MS. Meio de cultura isento de sacarose pode ser utilizado e nenhuma variação no perfil genético das plântulas foi observada em comparação com as plantas-mãe nas condições de cultura testadas. assexuada Entretanto K Portanto (MS disso RAPD. RAPD . (RAPD) tanto 25, 25 (25 5 100%, 100 100% , 100%) branca, (branca vermelha horas 2 C °C controle 1,5 15 3,0 30 3 0 45 4 4, 6,0%. 60 6,0% 6 6,0%) desenvolveramse desenvolveram se plantasmãe plantas mãe testadas (RAPD (2 10 1, 3, 6,0 ( 6,

ABSTRACT Kalanchoe daigremontiana is an ornamental species propagated exclusively asexually. However, no in vitro studies have been conducted to assess the relationship between changes in the culture environment and genetic variations of K. daigremontiana. Therefore, this study aimed to determine the optimal concentration of salts in the Murashige & Skoog (MS) medium and the optimal concentration of sucrose in the culture medium, in addition to the quality of light, in the development of in vitro plantlets from leaf explants of K. daigremontiana. Additionally, a genetic evaluation of the resulting plantlets was conducted to study phenotypic changes that could be attributed to somaclonal variation using Random Amplified Polymorphic DNA (RAPD) markers. For this purpose, leaf explants of K. daigremontiana were disinfected and inoculated in different salt concentrations (25, 50, and 100%) of MS medium without sucrose. The cultures were transferred to a growth chamber and subjected to three light conditions (white, blue, and red) with a 16-hour photoperiod, plus the absence of light, at a temperature of 27 ± 1 °C. Explants were also inoculated in 50% salt concentrations of MS medium supplemented with various concentrations of sucrose (control - without sucrose, 1.5, 3.0, 4.5, and 6.0%). K. daigremontiana leaf explants showed optimal development under white light and in any MS medium salt concentrations. A sucrose-free culture medium was viable, and no genetic variation was observed in the plantlets compared to the parent plants under the tested conditions. asexually However K Therefore (MS Additionally RAPD (RAPD markers purpose 25, 25 (25 50 100% 100 white, (white blue red 16hour hour 16 photoperiod 2 C °C control 15 5 1.5 30 3 0 3.0 45 4 4.5 6.0%. 60 6.0% . 6 6.0%) sucrosefree free viable (2 10 1. 3. 4. 6.0 ( 6.

RESUMO: Descrevemos os achados patológicos de um aborto causado por infecção por Yersinia enterocolitica em uma ovelha. Um feto ovino abortado, em estágio tardio de gestação, e a placenta correspondente foram submetidos à avaliação patológica. As alterações patológicas estavam restritas ao corioalantoide. Macroscopicamente, os cotilédones e as regiões intercotiledonárias estavam avermelhadas e os cotilédones estavam cobertos por pequena quantidade de fibrina. As lesões histológicas consistiam em placentite fibrinosupurativa necrosante multifocal severa, com vasculite fibrinoide e mineralização. A cultura bacteriana de amostras de pulmão, abomaso e placenta revelou crescimento puro de Y. enterocolitica. Este relato descreve o primeiro caso de infecção por Y. enterocolitica causando aborto em ovino no Brasil. RESUMO ovelha abortado gestação patológica corioalantoide Macroscopicamente fibrina severa mineralização pulmão Y Brasil

ABSTRACT: We described the pathological findings of an abortion associated with Yersinia enterocolitica infection in an ewe. A late-gestation aborted ovine fetus and placenta were submitted for necropsy. Pathologic changes were restricted to the chorioallantois. Grossly, cotyledons and intercodilenary regions had areas of mild red discoloration, and cotyledons were covered with a small amount of fibrin. Histological lesions consisted of multifocal, severe necrotizing and fibrinosuppurative placentitis with fibrinoid vasculitis and mineralization. Bacterial culture of lung, abomasum, and placenta samples yielded pure growth of Y. enterocolitica. This report described the first case of infection by Yersinia enterocolitica causing ovine abortion in Brazil. ABSTRACT ewe lategestation late gestation necropsy chorioallantois Grossly discoloration fibrin multifocal mineralization lung abomasum Y Brazil

ABSTRACT Introduction: the COVID-19 pandemic presented challenges worldwide. The acceptance of vaccines in the population is essential as a preventive measure. It is undeniable that there are factors that increase rejection or questioning in this regard, which may be related to culture, politics, trust, convenience, experience in health services, and also the opinion of the environment. Objective: to determine the percentage of students and teachers vaccinated, and the number of doses applied against COVID-19 in Facultad de Odontología at the Universidad Nacional de Asunción, in the year 2022. Methodology: cross-sectional descriptive observational study, applying a digital survey with 23 questions about knowledge, application and self-perception of the vaccine; in addition to demographic data. Results: 168 respondents were included, 128 students and 40 teachers. 80.0 % were women. 97.6 % have been vaccinated against COVID-19, the majority (85.4 %) received three doses of the vaccine. 34.1 % received the Covaxin vaccine, 32.9 % Sputnik V, 15.9 % AstraZeneca and 17.1 % others (Pfizer, Sinopharm and Moderna). 35.1 % had doubts about the effectiveness but most of them were applied anyway. 94.1 % considered the vaccine necessary and 88.7 % safe. 78.5 % responded that coverage is high to prevent the symptoms of the disease. 35.1% mentioned feeling fully protected with the vaccine. 78.6% know that some adverse reaction may occur after application. Conclusion: almost all of the respondents were vaccinated and had received all three doses of the COVID-19 vaccine at the time of the survey.

RESUMEN Introducción: la pandemia por COVID-19 presentó desafíos a nivel mundial. La aceptación de las vacunas en la población es indispensable como medida preventiva. Es innegable que existen factores que elevan el rechazo o cuestionamiento al respecto, que pueden estar relacionados con la cultura, política, confianza, conveniencia, experiencia en servicios sanitarios, también la opinión del entorno. Objetivo: determinar el porcentaje de estudiantes y docentes vacunados, y el número de dosis aplicadas contra el COVID-19 de la Facultad de Odontología de la Universidad Nacional de Asunción, en el año 2022. Metodología: estudio observacional descriptivo de corte transversal, aplicando una encuesta en digital con 23 preguntas acerca del conocimiento, aplicación y autopercepción de la vacuna; además de datos demográficos. Resultados: fueron incluidos 168 encuestados, 128 estudiantes y 40 docentes. 80,0 % fueron mujeres. 97,6 % se ha vacunado contra COVID-19, la mayoría (85,4 %) se aplicó tres dosis de la vacuna. 34,1 % se aplicó la vacuna Covaxin, 32,9 % Sputnik V, 15,9 % AstraZeneca y 17,1 % otras (Pfizer, Sinopharm y Moderna). El 35,1 % tuvo dudas acerca de la eficacia pero que en su mayoría se aplicaron igualmente. El 94,1 % consideró necesaria y el 88,7 % segura la vacuna. El 78,5 % respondió que la cobertura es elevada para prevenir los síntomas de la enfermedad. El 35,1 % mencionó sentirse protegido en su totalidad con la vacuna. El 78,6 % sabe que puede presentarse luego de la aplicación alguna reacción adversa. Conclusión: casi la totalidad de los encuestados se encontraban vacunados y se aplicaron las tres dosis de la vacuna COVID-19 al momento de la encuesta.

Resumen: Este artículo analiza la centralidad de las políticas públicas de seguridad y justicia en América Latina y el Caribe a partir de diversas técnicas de investigación teórico (análisis documental y bibliográfico) y trabajo de campo (visitas “in loco” y observaciones participantes), realizadas entre 2015 y 2021. Se parte del supuesto de que la violencia y las inseguridades son abordadas como un obstáculo flagrante para el desarrollo humano y la consolidación del estado de derecho en la región. Se identifica, basado en la literatura especializada y estudio comparado de estas políticas públicas, la relevancia del papel que juega el liderazgo político del estado y el compromiso cívico de varios actores sociales en torno a una gobernanza territorial integrada e integral de políticas de seguridad pública Ciudadana dedicados a salvar y preservar vidas. Resumen análisis bibliográfico visitas in loco participantes, participantes , participantes) 201 2021 región identifica vidas 20 202 2

Abstract: This article analyzes the centrality of public security and justice policies in Latin America and the Caribbean based on various theoretical research techniques (documental and bibliographical analysis) and fieldwork (“in loco” visits and participant observations), undertaken between 2015 and 2021. It starts from the assumption that violence and insecurities are approached as a flagrant obstacle to human development and the consolidation of the rule of law in the region. It is identified, in the comparison of the specialized literature and in the comparative study of these public policies, the relevance of the role played by the political leadership of the State and the civic engagement of several social actors around an integrated and integral territorial governance of citizen public security policies. Despite a punitive political culture and a history of authoritarian governments in the region, there are, after all, key principles and concepts for a new type of governance of citizen security (and justice) in Brazil and in the region aimed at saving and preserve lives. Abstract documental analysis loco observations, observations , observations) 201 2021 identified all lives 20 202 2

Resumo: Este artigo analisa a centralidade das políticas públicas de segurança e justiça na América Latina e no Caribe com base em diversas técnicas de pesquisa teórica (análise documental e bibliográfica) e de campo (visitas “in loco” e observações participantes), empreendidas entre 2015 e 2021. Parte-se do pressuposto de que as violências e inseguranças são abordadas como um flagrante obstáculo ao desenvolvimento humano e à consolidação do estado de direito na região. Identifica-se, no cotejo da literatura especializada e no estudo comparado dessas políticas públicas, a relevância do papel desempenhado pela liderança política do estado e pelo engajamento cívico de diversos atores sociais em torno de uma governança territorial integrada e integral das políticas de segurança pública cidadã. Inobstante a uma cultura política punitiva e a um histórico de governos autoritários na região, vislumbram-se, afinal, princípios e conceitos-chave para uma governança de novo tipo da segurança cidadã (e justiça) no Brasil e na região voltada a salvar e a preservar vidas. Resumo análise bibliográfica visitas in loco participantes, participantes , participantes) 201 2021 Partese Parte se Identificase, Identificase Identifica se, Identifica-se vislumbramse, vislumbramse vislumbram vislumbram-se afinal conceitoschave conceitos chave vidas 20 202 2

Abstract Introduction: Vascular and neuropathic complications of diabetes cause diabetic foot, being the main risk factor for non-traumatic amputations. Objective: To analyze the available scientific evidence on lifestyle and self-care of patients at risk of diabetic foot. Methodology: Literature review using the preferred reporting items for systematic reviews and meta-analyses methodology; published in EBSCO host, Google academic, PubMed, Scielo and Elsevier; in English and Spanish from 2016 to 2022. Nursing articles were included, mentioning two or more variables of interest: diabetes mellitus, lifestyle, diabetic foot, and self-care; duplicates, no access, and gray literature were eliminated. From a total of 1,080 articles, 14 were included that met the inclusion criteria. Checklists were used to assess the quality of the manuscripts used. Results: Systematic reviews, observational, experimental and qualitative studies showed evidence that self-care can be influenced by culture, context and knowledge deficit, being this a risk factor for the development of diabetic foot. An association was found between the risk of diabetic foot with years of diagnosis, inadequate self-care of the feet and advanced age. Conclusion: The association between the self-care deficit and the risk of diabetic foot in the elderly was evidenced. It is recommended to broaden the search and include people under 40 years of age in order to establish effective preventive measures and reduce the risk of diabetic foot.

Abstrato Introdução: As complicações vasculares e neuropáticas da diabetes causam o pé diabético, sendo o principal fator de risco para amputações não traumáticas. Objetivo: Analisar a evidência científica disponível sobre o estilo de vida e o autocuidado dos doentes em risco de pé diabético. Metodologia: Revisão da literatura utilizando a metodologia preferred reporting items for systematic reviews and meta-analyses; publicada no EBSCO host, Google Scholar, PubMed, Scielo e Elsevier; em inglês e espanhol de 2016 a 2022. Foram incluídos artigos de enfermagem que mencionavam duas ou mais variáveis de interesse: diabetes mellitus, estilo de vida, pé diabético e autocuidado; foram removidos os duplicados, sem acesso e literatura cinzenta. De um total de 1.080 artigos, 14 que preencheram os critérios de inclusão foram incluídos. Foram utilizadas listas de verificação para avaliar a qualidade dos manuscritos utilizados. Resultados: Revisões sistemáticas, estudos observacionais, experimentais e qualitativos mostraram evidências de que o autocuidado pode ser influenciado pela cultura, contexto e défices de conhecimento, que são factores de risco para o desenvolvimento da doença do pé diabético. Foram encontradas associações entre o risco de pé diabético e os anos de diagnóstico, o autocuidado inadequado dos pés e a idade avançada. Conclusão: Verificou-se a associação entre défices de autocuidado e o risco de doença do pé diabético em idosos. Recomendamos o alargamento da pesquisa a pessoas com menos de 40 anos de idade, de forma a estabelecer medidas preventivas eficazes e reduzir o risco de doença do pé diabético.

Resumen Introducción: Las complicaciones vasculares y neuropáticas de la diabetes causan el pie diabético, siendo el principal factor de riesgo para amputaciones no traumáticas. Objetivo: Analizar la evidencia científica disponible sobre el estilo de vida y autocuidado de los pacientes con riesgo de pie diabético. Metodología: Revisión de la literatura mediante la metodología preferred reporting items for systematic reviews and meta-analyses; publicada en EBSCO host, Google académico, PubMed, Scielo y Elsevier; en inglés y español desde 2016 a 2022. Se incluyeron artículos de enfermería, que mencionaran dos o más variables de interés: diabetes mellitus, estilo de vida, pie diabético y autocuidado; se eliminaron duplicados, sin acceso abierto y literatura gris. De un total de 1,080 artículos se incluyeron 14 que reunieron los criterios de inclusión. Se emplearon listas de verificación para evaluar la calidad de los manuscritos utilizados. Resultados: Las revisiones sistemáticas, estudios observacionales, experimentales y cualitativos mostraron evidencias de que el autocuidado puede verse influenciado por la cultura, contexto y déficit de conocimientos, siendo este factor de riesgo para el desarrollo de pie diabético. Se encontró asociación entre el riesgo de pie diabético con años de diagnóstico, autocuidado inadecuado de los pies y edad avanzada. Conclusión: Se evidenció asociación entre déficit de autocuidado y riesgo de pie diabético en el adulto mayor. Se recomienda ampliar la búsqueda e incluir personas menores de 40 años para establecer medidas preventivas eficaces y reducir el riesgo de pie diabético.

Abstract This research aims to identify the main factors that have been studied about the marketing of services and guest satisfaction in the hotel industry through a review of the literature during the period 2003 to 2023. The research path that was considered was of a qualitative order, since the first attempt was to discover and refine research questions until the main components around the study variables were identified using the database through the LENS platform. ORG, and the use of the VOSviewer tool to carry out bibliometric networks. Two clusters were identified that, through a bibliometric network directed towards the incidence of marketing in the hotel field with emphasis on the hospitality industry, the main study components were distinguished. It can be affirmed that, by effectively linking the advocacy component to human resources towards the organizational goals and objectives, as well as towards the adaptation of the prevailing organizational culture, people can be recruited, developed, encouraged, and retained to gain a competitive advantage; therefore, the success at a strategic and tactical level of a company immersed in the hotel industry.

Resumen Esta investigación tiene como objetivo identificar los principales factores que se han estudiado acerca de la mercadotecnia de servicios y la satisfacción del huésped en la hotelería a través de una revisión de la literatura durante el periodo 2003 a 2023. La ruta de investigación que se consideró fue de orden cualitativo, ya que, se trató primero de descubrir y refinar preguntas de investigación hasta identificar los principales componentes alrededor de las variables de estudio usando la base de datos a través de la plataforma LENS. ORG, y, el uso de la herramienta VOSviewer para realizar redes bibliométricas. Se identificaron dos clústeres que, a través una red bibliométrica dirigida hacia la incidencia de la mercadotecnia en el ámbito hotelero con énfasis en la industria de la hospitalidad se distinguieron los principales componentes de estudio. Se puede afirmar que, al vincular de forma efectiva el componente de incidencia a los recursos humanos hacia las metas y objetivos organizacionales, así también, como hacia la adaptación de la cultura organizacional prevaleciente, las personas pueden ser reclutados, desarrollados, alentados y retenidos para obtener una ventaja competitiva; por lo tanto, el éxito a nivel estratégico y táctico de una empresa inmersa en la industria hotelera.

Abstract: This text proposes a methodology for teaching and learning the history of architecture, based on the temporal influence of certain legitimizing media as a source of interpretation of architectural languages. The resulting pedagogy is based on the immediate, the mediatic and the mediatized forms, derived from research in architectural communication that analyzes the acceptance speeches of the Pritzker Architecture Prize between 1979 and 2015 and their impact on contemporary architectural culture. It examines the architectural signs enunciated by Luis Barragán and compares them with those of other editions, crossing the information gathered with other approaches, such as that of the languages of the architectural collective imaginary in different contexts, to obtain an alternative didactic for this type of readings. The resulting non-canonical teaching methodology interweaves theories, concepts or terms that reflect the understanding of architecture at a specific time thanks to the work of certain media actors.

Resumen: Este texto propone una metodología para enseñar y aprender la historia de la arquitectura, basada en la influencia temporal de ciertos medios de legitimación como fuente de interpretación de lenguajes arquitectónicos. La pedagogía resultante se fundamenta en lo inmediato, lo mediático y lo mediatizado, derivando de una investigación en comunicación arquitectónica que analiza los discursos de aceptación del Premio Pritzker de arquitectura entre 1979 y 2015 y su impacto en la cultura arquitectónica contemporánea. Se examina los signos arquitectónicos enunciados por Luis Barragán y se compara con el de otras ediciones, cruzando la información recopilada con otros enfoques, como el de los lenguajes del imaginario colectivo arquitectónico en diferentes contextos, para obtener una didáctica alternativa para este tipo de lecturas. La metodología docente resultante, no canónica, entrelaza teorías, conceptos o términos que reflejan la comprensión de la arquitectura en un momento específico gracias a la labor de ciertos actores mediáticos.

Abstract Introduction. Endophytic fungi (EF) in grapevines (Vitis vinifera) have great potential for agricultural applications and may represent possible biological control agents for vineyards. Objective. To isolate and identify endophytic fungi (EF) from grapevine plants with antibiosis capacity against Botrytis cinerea. Materials and methods. Young and mature branches from forty healthy grapevine plants were collected from seven production lots in the Cañete province, Lima, Peru, during March to May 2022. EF isolation was performed on 702 sections of disinfected tissues using potato dextrose agar (PDA) culture medium, and identification was carried out at the genus level. Secondary metabolites were extracted from twenty-eight EF strains to determine the mycelial inhibition of the pathogen. An analysis of variance (ANOVA) was conducted on the evaluated parameters using the Minitab statistical program. Results. A total of 129 EF strains were isolated from leaves and stems of young and mature grapevine branches, encompassing seven genera (Trichoderma, Aspergillus, Alternaria, Cladosporium, Fusarium, Nigrospora, and Stemphylium). The highest frequency was found in leaves and mature branches. Trichoderma and Alternaria were the most frecuent genera in grapevine plants, with 22.48 % and 21.71%, respectively. Metabolites obtained from the EFVH-01 strain of the genus Cladosporium, and EF-99 and EF-70 strains of the genus Alternaria, had the greatest effect on mycelial inhibition of the pathogen. Conclusions. Strains of endophytic fungi from the genera Cladosporium and Alternaria showed antibiosis capacity against Botrytis cinerea at the laboratory scale, which could potential biocontrol agents of pathogens in grapevine cultivation.

Resumen Introducción. Los hongos endófitos (HE) de la vid (Vitis vinifera) tienen un gran potencial de aplicación en la agricultura y pueden representar posibles agentes de control biológico de los viñedos. Objetivo. Aislar e identificar los hongos endófitos (HE) de plantas de vid con capacidad de antibiosis hacia Botrytis cinerea. Materiales y métodos. Se colectaron ramas jóvenes y maduras de cuarenta plantas sanas de vid de siete lotes de producción en la provincia de Cañete, Lima, Perú, durante los meses de marzo a mayo 2022. El aislamiento de HE se realizó de 702 secciones de tejidos desinfectados en medio de cultivo papa dextrosa agar (PDA) y la identificación fue a nivel de género. Se extrajeron metabolitos secundarios de veintiocho cepas de HE para determinar la inhibición micelial del patógeno. Se llevó a cabo un análisis de varianza (ANDEVA) de los parámetros evaluados con el programa estadístico Minitab. Resultados. Se aislaron un total de 129 cepas de HE de hojas y tallos de ramas jóvenes y maduras de vid, que correspondieron a siete géneros (Trichoderma, Aspergillus, Alternaria, Cladosporium, Fusarium, Nigrospora y Stemphylium). La mayor frecuencia se presentó en las hojas y rama madura. Trichoderma y Alternaria fueron los géneros más frecuentes en las plantas de vid con 22,48 % y 21,71 %, respectivamente. Los metabolitos obtenidos de las cepas HEVH-01 del género Cladosporium, HEV-99 y HEV-70 del género Alternaria, tuvieron mayor efecto en la inhibición micelial del patógeno. Conclusiones. Cepas de hongos endofitos del género Cladosporium y Alternaria mostraron capacidad de antibiosis hacia Botrytis cinerea a escala de laboratorio, los cuales podrían ser potenciales biocontroladores de patógenos en el cultivo de vid.

Resumen En el presente artículo se documenta y analiza la historia de La Casa Real-Imperial de Tijuana desde su fundación en 1982 hasta nuestros días. Se explora la posición de esta agrupación en la historia de la movilización LGBTIQ+ en la región y se propone el análisis de un circuito particular de espectáculos travesti o drag mediante su estudio como organización gay-travesti que apoyaba proyectos comunitarios LGBTIQ+. Con base en la investigación de los Archivos de la Sociedad Histórica GLBT de la ciudad de San Francisco, se problematiza la conformación de espacios de sociabilidad, solidaridad y ayuda mutua mediante la compleja parodia –de género y de la realeza– que realizaban emperatrices y emperadores de La Casa de Tijuana. En este marco, se evidencian puentes de comunicación transfronteriza poco documentados y se aportan reflexiones sobre los espacios de libertad y, a la vez, los rasgos de colonialidad de la subcultura gay-travesti.

Abstract This article proposes to analyze the history of the Imperial-Royal Casa de Tijuana since its foundation in 1982. It explores the position of this group in the history of LGBTIQ+ mobilization in this region and proposes the analysis of a particular circuit of travesti and drag shows, through its study as a gay- travesti organization that supported LGBTIQ+ community projects. Based on research from the GLBT Historical Society Archives of San Francisco, this work studies the formation of spaces of sociability, solidarity, and mutual aid through the complex parody—of gender and royalty—carried out by the Empresses and Emperors of the Casa de Tijuana. This framework shows cross-border communication bridges and provides reflections about the spaces of freedom, as well as the colonial traits embedded in gay-travesti subculture.

Abstract: In the current situation marked by a necessary ecosocial transition, the new urban agendas have been acquiring a leading role, establishing strategies for the transformation of cities based on the principles of sustainability, where the cultural approach takes on a relevant role. The aim of this article is to analyze, the framework agendas drawn up at different levels of government, from the perspective of heritage and culture. To this end, the documents that make up the International, European, Spanish and Andalusian urban agendas are analyzed by means of a bibliographical review, considering heritage and citizenship as key components in the urban configuration. As a result of the multilevel analysis, a series of key factors are identified as a reference framework for the implementation of new urban agendas in cities from a heritage and citizenship approach.

Resumen: En la actual coyuntura marcada por una necesaria transición ecosocial, las nuevas agendas urbanas han ido adquiriendo un papel protagonista, estableciendo estrategias para la transformación de las ciudades basadas en los principios de la sostenibilidad, donde el enfoque cultural adquiere un papel relevante. El objetivo de este artículo es analizar, desde el punto de vista del patrimonio y la cultura, estas agendas marco elaboradas en diferentes niveles de gobierno como el internacional, europeo, nacional y regional. Para ello, se realiza una revisión bibliográfica de los documentos que conforman las agendas urbanas Internacional, Europea, Española y Andaluza, considerando el patrimonio y la ciudadanía como componentes clave en la configuración urbana. Como resultado del análisis multinivel, se identifican una serie de factores clave como marco de referencia para la implementación de nuevas agendas urbanas en las ciudades desde un enfoque de patrimonio y ciudadanía.

ABSTRACT Due to the reduction of Chondracanthus chamissoi natural beds in Peru, different alternatives have been proposed for its cultivation, generally opting for suspended (CS) and bottom (CF) cultivation systems. When thalli reach commercial size, they get harvested for sale, and it is unknown if their specific growth rate (TCE) and/or regenerative capacity decrease. Therefore, the present work aimed to determine the TCE in the post-harvest regenerative capacity and the life cycle phase dominance of C. chamissoi in two pruning events comparing CS and CF during august 2019 and february 2020 in the south-central coast of Peru. The TCE was higher in CS (1.67 % day-1) in comparison to CF (1.47 % day-1). Likewise, CS showed better results in biomass (631.6 ± 81.3 gm-1) and length (23.91 ± 5.8 cm) compared to CF (256.5 ± 33.6 gm-1 and 21.10 ± 7.50 cm) during the first harvest. After the second harvest, lower values in biomass (CS: 160.2 ± 180 gm-1, CF: 59.4 ± 110 gm-1) and length (CS: 16.23 ± 3.68 cm, CF: 15.73 ± 4.25 cm) were observed. The dominant phase was the vegetative one, followed by the carposporic and tetrasporic phases. In conclusion, suspended cultivation showed better results; it is recommended to leave an average of 4-5 cm during pruning, to allow a better growth for a new harvest in less than 3 months.

RESUMEN Ante la reducción de praderas naturales de Chondracanthus chamissoi en Perú se han planteado diferentes alternativas para su cultivo, principalmente optando por sistemas de cultivo suspendido (CS) y cultivo de fondo (CF). Al alcanzar la talla comercial, los talos sufren podas para su comercialización, desconociéndose si disminuyen su tasa de crecimiento especifica (TCE) y/o capacidad regenerativa. Por ello, el presente trabajo tuvo como objetivo determinar la TCE en la capacidad regenerativa post-cosecha y la dominancia de fases del ciclo de vida de C. chamissoi en dos eventos de poda comparando CS y CF entre agosto de 2019 y febrero de 2020 en la costa centro-sur del Perú. La TCE fue mayor en CS (1.67 % día-1) respecto de CF (1.47 % día-1). Asimismo, CS mostró mejores resultados en biomasa (631.6 ± 81.3 gm-1) y longitud (23.91 ± 5.8 cm) en comparación a CF (256.5 ± 33.6 gm-1 y 21.10 ± 7.50 cm) tras la primera poda. Luego de la segunda poda, se observaron valores reducidos en biomasa (CS: 160.2 ± 180 gm-1, CF: 59.4 ± 110 gm-1) y longitud (CS: 16.23 ± 3.68 cm, CF: 15.73 ± 4.25 cm). Se determino a la fase vegetativa como dominante, seguida de talos carpospóricos y finalmente los tetraspóricos. Se concluye que CS muestra mejores TCE luego de eventos poda y se recomienda dejar en promedio 4-5 cm de talo para facilitar una rápida regeneración.

Resumo: As habilidades cognitivas das crianças variam conforme os contextos de desenvolvimento cultural em que elas se desenvolvem. Partindo do pressuposto da variabilidade cultural, este estudo teve como objetivo comparar as habilidades executivas de 110 crianças, com idades entre 9 e 11 anos, pertencentes a três grupos: não mapuche urbanas, mapuche urbanas e mapuche rurais, de municípios da região de La Araucanía, Chile. Foi utilizado um desenho descritivo e correlacional para comparar o desempenho das crianças nas variáveis de interesse. A bateria de instrumentos foi composta por três testes que avaliaram: atualização, mudança entre conjuntos mentais e inibição, respectivamente. Os resultados indicam que não houve diferença estatisticamente significativa em atualização e mudança entre conjuntos mentais, mas houve significância estatística para as diferenças em inibição, com as crianças não mapuches apresentando maior inibição do que os outros dois grupos. Os resultados são discutidos de acordo com a hipótese de que o desenvolvimento de habilidades está relacionado às práticas cotidianas, demandas e características sociodemográficas dos contextos em que as crianças se desenvolvem.

Resumen: Las habilidades cognitivas de los niños varían conforme a los contextos de desarrollo cultural en los que se desenvuelven. Asumiendo la variabilidad cultural, este estudio tuvo por objetivo comparar las habilidades ejecutivas en 110 niños, entre 9 y 11 años, pertenecientes a tres grupos: no mapuche urbanos, mapuche urbanos y mapuche rurales, de comunas de la región de La Araucanía, Chile. Se usó un diseño descriptivo y correlacional para contrastar el desempeño de los niños en las variables de interés. La batería de instrumentos estuvo formada por tres pruebas que evaluaron: actualización, cambio entre conjuntos mentales e inhibición, respectivamente. Los resultados indican que no hubo diferencias estadísticamente significativas en actualización y cambio entre conjuntos mentales, pero sí hubo significancia estadística para las diferencias en inhibición; siendo los niños no mapuche quienes tuvieron mayor inhibición respecto de los otros dos grupos. Se discuten los hallazgos según la hipótesis de que el desarrollo de habilidades se relaciona con las prácticas cotidianas, demandas y características sociodemográficas de los contextos en los que los niños se desarrollan.

Abstract: Children's cognitive abilities differ according to the cultural development settings in which they are raised. Assuming cultural variability, this study compared the executive functions in 110 children, aged 9 to 11 years, belonging to three groups: urban non-Mapuche, urban Mapuche, and rural Mapuche, from communes in the Araucanía region, Chile. A descriptive and correlational design was used to contrast children's performance on the variables of interest. The battery of instruments comprised three tests that assessed updating, set-shifting, and inhibition, respectively. The results indicate no statistically significant differences in updating and set-shifting, but there was a statistical significance for differences in inhibition, with non-Mapuche children having greater inhibition than the other two groups. The findings are discussed according to the hypothesis that skill development is related to the daily practices, demands, and sociodemographic characteristics of the settings in which children are raised.

Abstract From a philosophical-political approach to culture isolated from the assumptions of a modern-colonial-capitalist space-time, this essay examines the production of diverse spatio-temporal regimes. Museums, as emergent products of the spatio-temporal device of modern exhibition, stress the tradition-transmission relationship. Tradition implies an operation of separation that sacralizes a territory and a historical narrative -even when shown as secularized- while unifying, stabilizing and hierarchizing the version of an abstract, absolute, progressive, linear space-time over multiple spaces and times which it intends to proscribe. This does not occur without an intrinsic relationship with transmission as a process that includes an impossible point of closure based on which the possibility of change, which tradition seeks to control, rests. Based on a case study of museification processes associated with the 100th Anniversary of the city of Río Grande (Tierra del Fuego, Antarctica and South Atlantic Islands, Argentina), this essay explores four spatio-temporal regimes, and identifies several operations (profanation, inoperability, others) dismantling the spatio-temporal hierarchies that fix tradition. In this sense, supplementation process as the thought of the alteration, that is, change in terms of event occurrence, stands out. Supplementation thus involves an emancipatory thought in action, which dismantles the hierarchies and inequalities in the processes of museification linked to modernity.

Resumen Desde un enfoque filosófico-político de la cultura distanciado de los supuestos del espacio-tiempo moderno-colonial-capitalista, este ensayo indaga la producción de diversos regímenes espacio-temporales. Los museos, como productos emergentes del dispositivo espacio-temporal de exhibición moderno, tensionan la tradición y la transmisión. La tradición implica una operatoria de separación que sacraliza un territorio y una historia -aun cuando se muestre secularizada- a la vez que unifica, estabiliza y jerarquiza la versión de un espacio-tiempo abstracto, absoluto, progresivo y lineal por sobre espacios-tiempos múltiples que pretende clausurar. Esto no se da sin una intrínseca relación con la transmisión como proceso que incluye un punto imposible de clausura. Sobre este se apoya la posibilidad del cambio al que la tradición pretende controlar. A partir de un estudio de caso sobre procesos de museificación asociados al centenario de la ciudad de Río Grande (Tierra del Fuego, Antártida e Islas del Atlántico Sur, Argentina), el presente ensayo problematiza cuatro regímenes espacio-temporales e identifica diversas operatorias (profanación, inoperosidad, otras) que desmontan las jerarquías espacio-temporales que fijan la tradición. Destaca la operatoria de suplementación como pensamiento de la alteración, es decir, del cambio en términos acontecimentales. Esta última involucra un pensamiento emancipador en acto, que desarma las jerarquías y las desigualdades en los procesos de museificación ligados a la modernidad.