Over the last two decades, metabolomics has emerged as a pivotal tool in multidisciplinary research, providing invaluable insights into metabolome modulations and finding applications across various scientific domains, including medicine and agronomy. Nonetheless, the absence of standardized procedures in sample preparation, data acquisition, and documentation of data quality presents a significant challenge. This review underscores the critical importance of quality assurance and quality control (QA/QC) in untargeted metabolomics, advocating for the establishment of agreed-upon QA/QC reporting standards within the scientific community. We discuss the requisite quality controls at various stages of untargeted metabolomics studies, encompassing blank samples, pooled samples, and the utilization of external quality control samples. Methods for assessing accuracy, reproducibility, and identifying/correcting batch effects are addressed. Furthermore, emphasis is placed on standardizing the description of QA/QC data in scientific publications and repositories to foster reproducibility and transparency. We recommend the publication of QC data alongside studies in appropriate databases to facilitate data comparison and sharing among researchers, thereby enhancing the quality of untargeted metabolomics research. In summary, implementing standardized QA/QC data reporting, along with promoting best practices within the untargeted metabolomics community, is crucial for improving result quality and credibility and advancing research utilizing this powerful technique. decades domains agronomy Nonetheless preparation acquisition challenge QAQC QA (QA/QC agreedupon agreed upon community samples accuracy identifyingcorrecting identifying correcting addressed Furthermore transparency researchers summary technique

RESUMO A introdução da soja tolerante a 2,4-D (ENLIST®) oferece um novo potencial para a aplicação de herbicidas. Dessa forma, o presente trabalho teve como objetivo: (a) avaliar o efeito das combinações de herbicidas pós-emergentes no controle de Conyza spp., e (b) a seletividade da associação de herbicidas pós-emergentes em soja, garantindo uma aplicação eficaz sem comprometer a saúde da cultura. Um experimento de campo com soja tolerante ao 2,4-D utilizou um delineamento experimental de blocos casualizados, com 14 tratamentos em quatro repetições. Os tratamentos incluíram várias combinações de 2,4-D com glifosato, chlorimuron, cloransulam, imazethapyr, bentazona e imazamox. Estes foram aplicados como um único tratamento ou sequencialmente 15 dias após o tratamento (DAT) usando glufosinato de amônio, juntamente com dois controles sem aplicação de herbicida. Os resultados mostraram menos de 15% de fitotoxicidade em todos os tratamentos. Aos 21 DAT, apenas os tratamentos aplicados sequencialmente mostraram controle significativo, com eficácia superior a 80% contra Conyza spp. Em contraste, as aplicações únicas das combinações de herbicidas com 2,4-D foram ineficazes para o controle pós-emergência de Conyza spp. 2,4D 24D D 2,4 2 4 ENLIST® ENLIST (ENLIST® forma objetivo (a pósemergentes pós emergentes spp b (b cultura casualizados 1 repetições glifosato chlorimuron cloransulam imazethapyr imazamox DAT (DAT amônio herbicida significativo 80 contraste pósemergência emergência 4D 24 2, (ENLIST 8

ABSTRACT The introduction of 2,4-D-tolerant soybeans (ENLIST®) offers a new potential for herbicide application. Therefore, the present study aimed to (a) evaluate the effect of combinations of post-emergent herbicides on the control of Conyza spp. and (b) assess the selectivity of the post-emergent herbicide association in soybeans, ensuring effective application without compromising the health of the crop. A field experiment with 2,4-D-tolerant soybeans utilized a randomized complete block design with 14 treatments across four replications. Treatments included various combinations of 2,4-D with glyphosate, chlorimuron-ethyl, cloransulam-methyl, imazethapyr, bentazon, and imazamox. These were applied either as a single treatment or sequentially 15 days after treatment (DAT) using glufosinate-ammonium alongside two controls without herbicide application. The results showed less than 15% control across all treatments. At 21 DAT, only the treatments applied sequentially showed significant control, with effectiveness exceeding 80% against Conyza spp. In contrast, the single applications of the herbicide combinations with 2,4-D were ineffective for post-emergence control of Conyza spp. 2,4Dtolerant 24Dtolerant Dtolerant 2,4 D tolerant 2 4 ENLIST® ENLIST (ENLIST® Therefore (a postemergent post emergent spp b (b crop 1 replications 2,4D 24D glyphosate chlorimuronethyl, chlorimuronethyl chlorimuron ethyl, ethyl chlorimuron-ethyl cloransulammethyl, cloransulammethyl cloransulam methyl, methyl cloransulam-methyl imazethapyr bentazon imazamox DAT (DAT glufosinateammonium glufosinate ammonium 80 contrast postemergence emergence 4Dtolerant 24 2, (ENLIST 4D 8

ABSTRACT Purpose: To assess the impact of thinness on the outcome of the percutaneous nephrolithotomy (PCNL). Materials and Methods: A matched case–control study was performed using a prospectively collected database of all patients who underwent PCNL between June 2011 and October 2021. The patients were stratified into two groups according to their phenotypic characteristics, arbitrarily defined according to their body mass index (BMI): <20 kg/m2 (Group 1, very thin patients, G<20) and ≥25 kg/m2 (Group 2, non-thin patients, G≥25). Patients were randomly matched based on Guy's Stone Score (GSS) according to case complexity at a ratio of 1:3. Results: A total of 204 patients were enrolled in this study: 51 patients (G<20) and 153 controls (G≥25). Complications occurred in 15.2% of the patients, with 5.4% of these complications classified as major complications (Clavien grade ≥ 3). According to complications there were no significant differences between the groups. The overall complication rates were 17.6% in the G<20 and 14.4% in the G≥25 (p = 0.653). The major complication rates were 3.9% in the G<20 and 5.8% in the G≥25 (p=0.429). No differences in transfusion or urinary fistula rates were found. Conclusions: In this study, very thin patients were not at a higher risk of complications when submitted to PCNL than in those with a BMI of ≥25 kg/m2. Apparently, this technique can be used in these patients, just as it is used in any other type of patient, independently of their BMI. Purpose PCNL. . (PCNL) Methods casecontrol control 201 2021 characteristics (BMI) 20 <2 kgm2 kgm kg m2 m kg/m Group 1 G20 G 25 ≥2 2 nonthin non G≥25. G25 G≥25) Guys Guy s GSS (GSS 13. 13 3. 3 1:3 Results 5 (G<20 15 (G≥25) 152 15.2 54 4 5.4 Clavien 3) 176 17 6 17.6 G<2 144 14 14.4 G≥2 p 0.653. 0653 0.653 0 653 0.653) 39 9 3.9 58 8 5.8 p=0.429. p0429 p=0.429 429 (p=0.429) found Conclusions Apparently patient (PCNL 202 (BMI < G2 1: (G<2 (G≥25 15. 5. 17. G< 14. G≥ 065 0.65 65 p042 p=0.42 42 (p=0.429 (G< (G≥2 06 0.6 p04 p=0.4 (p=0.42 (G (G≥ 0. p0 p=0. (p=0.4 p=0 (p=0. p= (p=0 (p=

Abstract Introduction. Evaluating onion cultivars (Allium cepa L.) in different environments to understand their adaptation is important due to the crop’s relevance in food supply across various countries, the reduction in its genetic diversity, the loss of beneficial traits, and resistance to diseases and pests. Objective. To assess the agronomic performance of onion cultivars under different environments to identify the best ones in terms of adaptation, yield, and interaction. Materials and methods. From November 2017 to August 2018, at the La Selva Research Center of the Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), Rionegro, Antioquia, Colombia, twenty-one onion cultivars plus two controls were evaluated: Peruana of the Aggregatum group and Yellow Granex of the Cepa, under open-field and protected conditions with plastic roofing, using multilocation trials in a randomized complete block design and four replications. Evaluations were consucted on categories such as bulb weight, number, length, and diameter, along with the interaction of cultivars and environments. Results. In othe open field, due to lower temperatures compared to those recorded under protected conditions resulted in higher yields of weight and number of bulbs. Under protected environments, Aggregatum group cultivars behaved as if they were from the Cepa group, producing fewer large-size bulbs. The adaptation of cultivars varied by environment: in the open field, L55 and L63 cultivars exhibited the best yields, while under protected conditions, RPT20 and RPT11 showed the best adaptation. Conclusion. Environments significantly influenced the behavior of Aggregatum group cultivars, with higher production of extra-large bulbs. Temperature contrasts in protected enviroments with plastic roofing affected cultivars yields, while open-field conditions were more favorable.

Resumen Introducción. La evaluación de cultivares de cebolla (Allium cepa L.) en diferentes ambientes para conocer su adaptación es importante debido a la relevancia del cultivo en la alimentación en diferentes países, la reducción de su diversidad genética, la perdida de sus rasgos benéficos y la resistencia a enfermedades y plagas. Objetivo. Evaluar el comportamiento agronómico de cultivares de cebolla bajo diferentes ambientes, para identificar los mejores en adaptación, rendimiento e interacción. Materiales y métodos. De noviembre 2017 a agosto de 2018, en el Centro de Investigación La Selva de la Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), Rionegro, Antioquia, Colombia, se evaluaron veintún cultivares más dos testigos de cebolla: Peruana del grupo Aggregatum y Yellow Granex del Cepa, bajo condiciones de campo abierto y protegido con techo plástico, con ensayos multilocacionales en un diseño de bloques completos al azar y cuatro repeticiones. Se evaluó por categorías: peso, número, longitud y diámetro de los bulbos; junto con la interacción de los cultivares y los ambientes. Resultados. En campo abierto, por presentar temperaturas menores a las registradas en las condiciones protegidas, se obtuvieron los mayores rendimientos de peso y número de bulbos. En ambientes protegidos, los cultivares del grupo Aggregatum, se comportaron como si fueran del grupo Cepa, al producir pocos bulbos de gran tamaño. La adaptación de los cultivares fue diferente en los ambientes: en campo abierto los cultivares L55 y L63 presentaron los mejores rendimientos, mientras que bajo condiciones protegidas, RPT20 y RPT11 fueron los que mejor se adaptaron. Conclusión. Los ambientes influyeron significativamente en el comportamiento de los cultivares del grupo Aggregatum con una mayor producción de bulbos extragrandes. Los contrastes de temperaturas que se presentaron en los ambientes protegidos con techo plástico, afectaron los rendimientos de los cultivares, mientras que las condiciones ambientales a campo abierto fueron favorables.

Resumo: As funções executivas (FE) e a regulação emocional (RE) estão associadas à base dos desenvolvimentos cognitivo e socioemocional de crianças. Em vista da corrente de intervenções neuropsicológicas em FE, o presente estudo buscou investigar se uma intervenção de FE (programa PENcE) seria equivalente, superior ou inferior em relação à intervenção de FE em conjunto com estratégias de RE (programas PENcE e REPENcE) no desempenho executivo, emocional e escolar de alunos do Ensino Fundamental I que foram submetidos a programas de desempenho exclusivamente focados nas FE (PENcE) versus programa misto de FE e RE (PENcE+REPENcE) versus controles. Participaram 111 crianças, dividida em três grupos: grupo 1 recebeu uma intervenção mista com os programas PENcE e REPENcE, grupo 2 realizou somente o PENcE, e grupo 3 foi o grupo controle (currículo escolar). O grupo submetido à intervenção mista apresentou na avaliação pós-intervenção desempenho superior em um maior número de domínios, como, iniciação, velocidade de processamento, consciência corporal, e comunicação das emoções. Deste modo, percebe-se que um programa focado em habilidades de RE pode potencializar e incrementar os desfechos de um programa de estimulação cognitiva exclusivamente de FE.

Resumen: Las funciones ejecutivas (FE) y la regulación emocional (RE) se asocian a partir de los desarrollos cognitivos y socioemocionales de los niños. En vista de las intervenciones neuropsicológicas actuales en FE, el presente estudio buscó investigar si una intervención de FE (programa PENcE) sería equivalente, superior o inferior en relación a la intervención de FE en conjunto con estrategias de RE (programas PENcE y REPENcE) en el desempeño ejecutivo, emocional y académico de estudiantes de la escuela primaria sometidos a programas de desempeño enfocados exclusivamente en FE (PENcE) versus un programa mixto de FE y RE (PENcE+REPENcE) versus controles. Participaron 111 niños, divididos en tres grupos: el grupo 1 recibió una intervención mixta con los programas PENcE y REPENcE, el grupo 2 recibió solo PENcE, y el grupo 3 fue el grupo de control (currículo escolar). El grupo sometido a la intervención presentó un desempeño superior en la evaluación posintervención en un gran número de dominios, como iniciación, velocidad de procesamiento, conciencia corporal y comunicación de emociones. Así, es claro que un programa centrado en las habilidades de RE puede potenciar y aumentar el resultado de un programa de estimulación cognitiva exclusivamente de FE.

Abstract: Executive functions (EF) and emotion regulation (ER) are associated with the basis of children's cognitive and socio-emotional development. In view of the current of neuropsychological interventions in EF, the present study sought to investigate whether an EF intervention (PENcE program) would be equivalent, superior or inferior in relation to the EF intervention in conjunction with ER strategies (PENcE and REPENcE programs) in performance executive, emotional and school performance of elementary school students who underwent performance programs exclusively focused on EF (PENcE) versus a mixed EF and ER program (PENcE+REPENcE) versus controls. One hundred and eleven children participated, divided into three groups: group 1 received a mixed intervention with the PENcE and REPENcE programs, group 2 performed only the PENcE, and group 3 was the control group (school curriculum). The group submitted to the mixed intervention showed superior performance in a greater number of domains, such as initiation, processing speed, body awareness, and communication of emotions, in the post-intervention evaluation. Thus, it is clear that a program focused on ER skills can enhance and increase the outcomes of a cognitive stimulation program exclusively for EF.

Abstract Introduction: Bio bays in Puerto Rico play an important socio-economic role and declines in dominant bioluminescent dinoflagellate Pyrodinium bahamense are concerning. Studies show erratic blooms with is weak correlation to in situ environmental factors. Our study examines shorter field and longer proxy records on dinoflagellate density at Laguna Grande de Fajardo (LGF). Objectives: To quantify temporal changes in dinoflagellate density in a long-term monitoring study, understand how the marine environment modulates those changes, and determine the wider impacts of a fluctuating climate and extreme events on proxies for dinoflagellate density. Methods: Bimonthly samples were collected from 2016 to 2021 at three sampling sites in LGF. Dinoflagellates density was estimated by Sedgewick Rafter counting cells. Environmental conditions were obtained from Rio Fajardo 5007100 station and NOAA buoy 41056. Marine climate and biotic proxies were obtained from remote sensing measurements. Kruskal Wallis, Spearman correlations and cross-correlations in the shorter field and longer proxy records were used to evaluate environmental controls on LGF dinoflagellate blooms. Results: Six years of field monitoring densities found a low period in 2016-2017, frequent and intense blooms in 2018-2021 punctuated by hurricanes. Generally low values were recorded in late winter in contrast with higher values in late summer (Aug-Nov). Light winds and mixed layer response to seasonal warming in the form of high tides and low salinity, were found to sustain dinoflagellate reproduction. Conclusions: Bioluminescent dinoflagellates are vital to coastal tourism and require resource management. LGF results show that: 1) dinoflagellate counts fluctuate widely, 2) fluorescing dinoflagellates are sensitive to environmental conditions because of limited seasonality and narrow physiological range, 3) hurricanes play a role by ‘raking and refreshing’ the coastal lagoon for subsequent biotic reproduction, and 4) intra-seasonal fluctuations of density and proxies relate to air-sea thermodynamic conditions, the salinity budget and sea level.

Resumen Introducción: Las bahías bioluminiscentes en Puerto Rico desempeñan un papel socioeconómico importante y disminuciones del dinoflagelado bioluminiscente dominante Pyrodinium bahamense son preocupantes. Los estudios muestran proliferaciones erráticas débilmente correlacionadas con factores ambientales locales. Objetivos: Cuantificar cambios en la densidad de dinoflagelados a largo plazo en la Laguna Grande de Fajardo (LGF), comprender cómo el ambiente marino modula esos cambios y determinar los impactos de eventos climáticos extremos en la densidad de dinoflagelados. Métodos: Se recolectaron muestras bimestrales del 2016-2021 en tres sitios de la LGF. La densidad se estimó mediante el conteo de células de Sedgewick Rafter. Condiciones ambientales se obtuvieron de la estación Río Fajardo 5007100 y de la boya NOAA 41056. El clima marino y los indicadores bióticos se obtuvieron por mediciones de teledetección. Se utilizaron correlaciones Kruskal Wallis, Spearman y las correlaciones cruzadas en los registros de campo corto y proxy largos para evaluar los controles ambientales en las proliferaciones de dinoflagelados LGF. Resultados: En seis años de monitoreo se encontraron densidades bajas entre el 2016-2017 y proliferaciones frecuentes e intensas en 2018-2021 marcadas por huracanes. Se registraron valores bajos a finales del invierno, en contraste con valores más altos a finales del verano (agosto-noviembre). Vientos ligeros y la respuesta de capas mixtas al calentamiento estacional en forma de mareas altas y baja salinidad, mantienen la reproducción de los dinoflagelados. Conclusiones: Los dinoflagelados bioluminiscentes son vitales para el turismo costero y requieren manejo de los recursos. En general: 1) los recuentos de dinoflagelados fluctúan ampliamente, 2) los dinoflagelados fluorescentes son sensibles a las condiciones ambientales debido a su estacionalidad limitada y estrecho rango fisiológico, 3) los huracanes juegan un rol de rastrillar y refrescar la laguna costera para su posterior reproducción, y 4) fluctuaciones intra-estacionales se relacionan con las condiciones termodinámicas aire-mar, el balance de salinidad y el nivel del mar.

Direct current flow through power transformers in HVDC systems can lead to significant half-cycle saturation issues, putting the power system at risk. The HVDC system can function in monopolar ground return and unbalanced bipolar without earth return conductors. During these two HVDC modes of operation, a substantial direct current flows through the HVDC ground terminals, creating a ground DC potential difference between the neutrally grounded transformers. As a result, DC flows through the neutrals into the transformer windings. The study presents a transformer-neutral DC compensating device incorporating a novel control to solve the issue. Using a proper control strategy, injecting reverse DC into the grounding grid can compensate for direct current flow in transformer windings to mitigate the biased operating flux of power transformers. In this article, an in-depth analysis of transformer response to DC bias was investigated. Then, an Interval type-II fuzzy logic control (IT2FLC) was proposed as an effective control strategy for managing the neutral DC compensating system. Its robustness was assessed and analysed by comparing it with type-I fuzzy logic-based (T1FLC) and a PI-based compensation system. The control performance is examined using MATLAB/Simulink models and validated with rapid control prototype tests conducted with a SpeedgoatTM real-time target machine, assessing the transient response, oscillations, and settling time of the compensation device under DC bias voltage variations. The outcomes indicate that the IT2FLC controls the compensation device more effectively than other controllers to mitigate half-cycle saturation. This approach introduces a novel strategy to prevent transformer half-cycle saturation.

Resumen Introducción: Las investigaciones han demostrado la importancia de diferenciar el trastorno por déficit de atención con hiperactividad (TDAH) de las quejas subjetivas de dificultades de atención. En ambos casos existe una manifestación clínica que afecta diferencialmente a mujeres y hombres. Este estudio evaluó las posibles relaciones entre los síntomas conductuales y emocionales, el diagnóstico de TDAH, las quejas atencionales y el sexo en adultos jóvenes de entre 18 y 25 años. Método: Utilizando un diseño transversal, examinamos las relaciones entre tres grupos (diagnosticados con TDAH, quejas atencionales y controles) en una muestra de 232 participantes (Mediaedad = 20.38 años, DEedad = 1.81 años; 52 % mujeres). Empleamos la escala de Autoinforme de TDAH en Adultos y la prueba Toulouse Pieron para clasificar a los participantes entre grupos; para medidas de síntomas conductuales/emocionales utilizamos el inventario multifásico de personalidad de Minnesota-2 - Forma reestructurada (MMPI-2-RF), por medio de las escalas de Alto Orden y las Somáticas/ Cognitivas. Resultados: El análisis multivariado de varianza (Manova) mostró una relación significativa entre el grupo y las variables del MMPI-2-RF después de controlar el sexo (p < 0.01). Las pruebas post hoc indicaron diferencias entre grupos en todas las variables, excepto en quejas de dolor de cabeza. Además, las comparaciones entre hombres y mujeres revelaron diferencias significativas en la disfunción conductual y emocional y en las quejas gastrointestinales. Conclusión: Los hallazgos resaltan la importancia de realizar un diagnóstico que no se base únicamente en una lista de síntomas y signos, sino que también tenga en cuenta las diferencias de sexo, las alteraciones emocionales/conductuales y las conexiones asociadas con una mayor rumiación en adultos jóvenes, lo cual puede influir en el sesgo de autoevaluación y contribuir a una sobreestimación o subestimación de la sintomatología del TDAH.

Abstract Introduction: Research has shown the importance of differentiating Attention Deficit Hyperactivity Disorder (ADHD) from subjective complaints of attention difficulties. In both cases, there is a clinical manifestation that differentially affects women and men. This study assessed the potential relationships among Behavioural and Emotional Symptoms, ADHD Diagnosis, Attentional Complaints, and Sex in Young Adults between 18 and 25 years of age. Method: Using a cross-sectional design, we examined the relationships across three groups (diagnosed with ADHD, attentional complaints, and controls) in a sample of 232 participants (Meanage = 20.38 years, SDage = 1.81 years; 52% women). We employed the Adult ADHD Self Report Scale and the Toulouse Pieron Test to classify participants among groups; for measures of behavioural/emotional symptoms using the Minnesota Multiphasic Personality Inventory-2 - Restructured Form (MMPI-2-RF), through High-Order and Somatic/Cognitive Scales. Results: Multivariate Analysis of Variance (MANOVA) revealed a significant relationship between group membership and the MMPI-2-RF variables after controlling for sex (p < 0.01). Post hoc tests indicated group differences across all variables except for Head Pain Complaints. Additionally, comparisons between men and women revealed significant differences in Behavioural and Emotional Dysfunction, and Gastro-Intestinal Complaints. Conclusion: The findings highlight the importance of making a diagnosis that is not only based on a list of symptoms and signs but also takes into account differences in sex, emotional/behavioural alterations, and associated connections to increased rumination in young adults, which may influence self-assessment bias and contribute to overestimation or underestimation of ADHD symptomatology.

SUMMARY: Drinking alcohol while pregnant can cause several diseases, belonging to an umbrella of disorders known as Fetal Alcohol spectrum disorders. Alcohol exposure during pregnancy is linked to reduced birth weights, shorter bones, delayed bone growth, decreased bone resilience, and delayed ossification of the bones. However, the specific effects on the craniofacial bones, particularly the mandible, remain less explored. This study aims to fill this gap by examining the impact of gestational alcohol consumption on mandibular structure. Time-mated pregnant Sprague Dawley dams were divided into an ethanol group (n=6) and a saline control group (n=6). The ethanol group was given 0.015ml/g of 25.2% ethanol, while the control group received 0.9% saline for the first 19 days of gestation. The presence of a vaginal plug indicated the first day of gestation. After birth, the pups were euthanized at three weeks of age, and their mandibles were harvested and analyzed using a 3D-mCT scanner to assess bone ratio, trabecular thickness, number, and spacing. Osteometric measurements for mandibular length and height were also taken. The study found no significant differences in trabecular morphometric parameters between the groups. However, one parameter for mandibular length (Cd-Bi) and two parameters for mandibular height (Cd-Ag and Cr-Ag) were significantly smaller in the ethanol group than in the controls. The study reveals that gestational alcohol consumption can significantly affect the development of the mandible, with potential implications for understanding the mechanisms behind alcohol-induced teratogenicity. The findings highlight the need for further research to explore the intricate interactions between embryological, biological, genetic, and epigenetic factors that contribute to the varied effects of alcohol exposure during pregnancy on different skeletal systems.

RESUMEN: Beber alcohol durante el embarazo puede causar varias enfermedades, pertenecientes a un conjunto de trastornos conocidos como trastornos del espectro alcohólico fetal. La exposición al alcohol durante el embarazo está relacionada con un peso reducido al nacer, huesos más cortos, retraso en el crecimiento óseo, disminución de la resistencia ósea y retraso en la osificación de los huesos. Sin embargo, los efectos específicos sobre los huesos craneofaciales, particularmente la mandíbula, siguen siendo menos analizados. Este estudio tiene como objetivo llenar este vacío examinando el impacto del consumo de alcohol gestacional en la estructura mandibular. Las madres preñadas Sprague Dawley apareadas en el tiempo, se dividieron en un grupo de etanol (n=6) y un grupo control de solución salina (n=6). El grupo de etanol recibió 0,015 ml/g de etanol al 25,2 %, mientras que el grupo control recibió solución salina al 0,9 % durante los primeros 19 días de gestación. La presencia de un tapón vaginal indicó el primer día de gestación. Después del nacimiento, las crías fueron sacrificadas a las tres semanas de edad, y sus mandíbulas fueron extraídas y analizadas utilizando un escáner 3D-mCT para evaluar la proporción ósea, el grosor trabecular, el número y el espaciado. También se tomaron medidas osteométricas de longitud y altura mandibular. El estudio no encontró diferencias significativas en los parámetros morfométricos trabeculares entre los grupos. Sin embargo, un parámetro para la longitud mandibular (Cd-Bi) y dos parámetros para la altura mandibular (Cd-Ag y Cr-Ag) fueron significativamente menores en el grupo de etanol que en los controles. El estudio revela que el consumo de alcohol gestacional puede afectar significativamente el desarrollo de la mandíbula, con implicaciones potenciales para comprender los mecanismos detrás de la teratogenicidad inducida por el alcohol. Los hallazgos resaltan la necesidad de realizar más investigaciones para explorar las intrincadas interacciones entre factores embriológicos, biológicos, genéticos y epigenéticos que contribuyen a los diversos efectos de la exposición al alcohol durante el embarazo en diferentes sistemas esqueléticos.

Abstract: This research examines the incorporation of CuO and TiO2 nanoparticles into polypropylene or cotton fabrics to develop reusable personal protective equipment. Different preparation methods are applied to anchor the nanoparticles and avoid leaching. The composition and preparation of fabrics are verified by scanning electron microscopy and X-ray diffraction. Optical properties are assessed by diffuse reflectance spectroscopy and photoluminescence. The results show that for a low ratio of CuO to TiO2, the optical properties improve, reducing bandgap energy and the electron-hole pair recombination rate. Photodegradation of methylene blue increases by 22.8% in polypropylene fabrics and up to 350% in cotton fabrics. In conclusion, photoactivity in polypropylene fabrics is 23.7 times higher than in cotton fabrics, since the preparation method controls the loading of active species.

Resumen: Esta investigación examina la incorporación de nanopartículas de CuO y TiO2 sobre telas de polipropileno o algodón para su implementación como equipo de protección personal reusable. Se utilizan diferentes métodos de preparación para garantizar el anclaje de las nanopartículas y evitar su lixiviación. La composición y preparación de los textiles se verifica por microscopía electrónica de barrido y difracción de rayos X. Las propiedades ópticas se evalúan mediante espectroscopia de reflectancia difusa y fotoluminiscencia. Los resultados muestran que para una baja proporción de CuO a TiO2, se mejoran las propiedades ópticas de los textiles, reduciendo la energía de banda prohibida y la tasa de recombinación del par electrón-hueco. La fotodegradación del azul de metileno aumenta un 22.8% en textiles de polipropileno y hasta 350% en textiles de algodón. En conclusión, la fotoactividad en textiles de polipropileno es 23.7 veces mayor respecto al algodón, ya que con el método de preparación se controla el contenido de las especies activas.

Abstract Objective To compare the phase angle (PhA) through bioelectrical impedance (BIA) of children with intestinal failure (IF) using prolonged parenteral nutrition (PN) followed by an Intestinal Rehabilitation Program, with a control group. Methods Children under 10 years of age with IF using prolonged PN for >60 days (study group) were included. The control group consisted of healthy children without chronic pathologies, matched by sex and age. Anthropometric parameters evaluated were: weight, height, weight/age z-score (W/A), height/age z-score (H/A), BMI, BMI/A z-score, arm circumference, triceps skinfold, subscapular skinfold, mid-arm muscle circumference. BIA parameters were resistance (R), reactance (Xc), and phase angle (PhA). Results Twenty-eight children were included in the study group, median (IQR) age was 11 (8-27) months, 53.6 % were male. In the control group, 28 children were included, median (IQR) age was 12.5 (8-24.7) months, 50 % were male. Children from the study group had W/A z-scores and H/A z-scores significantly lower than controls. There was no significant difference between PhA in the study group and controls, [median (IQR) 4.3° (3.8;4.6) vs 4.0° (3.8;5.4) respectively, p = 0.980]. Prematurity was significantly higher in the study group than in the controls, but there was no significant correlation between gestational age at birth and PhA of the children from the study group. Conclusion Children with IF using prolonged PN showed lower W/A and H/A compared to the control group, but without significant difference between the PhA of children with IF compared to controls. (PhA (BIA (IF (PN Program 1 60 >6 pathologies weight height weightage zscore z score W/A, WA , W A (W/A) heightage H/A, HA H (H/A) BMI BMIA zscore, score, circumference skinfold midarm mid R, R (R) Xc, Xc (Xc) PhA. . Twentyeight Twenty eight IQR (IQR 827 8 27 (8-27 months 536 53 6 53. male 2 125 12 5 12. 824.7 8247 24.7 24 7 (8-24.7 zscores scores controls 43 4 3 4.3 3.84.6 3846 3.8 4.6 (3.8;4.6 40 0 4.0 3.85.4 3854 5.4 (3.8;5.4 respectively 0.980. 0980 0.980 980 0.980] > (W/A (H/A (R (Xc 82 (8-2 824 824. 247 24. (8-24. 4. 84 3.84. 384 38 3. 46 (3.8;4. 85 3.85. 385 54 5. (3.8;5. 098 0.98 98 (8- (8-24 3.84 (3.8;4 3.85 (3.8;5 09 0.9 9 (8 (3.8; 0. ( (3.8 (3. (3

SUMMARY OBJECTIVE: This study aimed to assess the prevalence of temporomandibular dysfunction in ankylosing spondylitis patients and healthy controls, examining the relationship between temporomandibular dysfunction and disease activity in ankylosing spondylitis patients, as well as associations with psychosocial factors. METHODS: The study included 113 ankylosing spondylitis patients and 110 healthy individuals aged 18–75. Temporomandibular dysfunction presence was evaluated using Diagnostic Criteria for Temporomandibular Disorders Axis I. Disease activity was assessed with the Bath Ankylosing Spondylitis Disease Activity Index, Bath Ankylosing Spondylitis Metrology Index, and Bath Ankylosing Spondylitis Functional Index. RESULTS: Among healthy individuals, 60.9% did not receive a temporomandibular dysfunction diagnosis, while 39.1% received at least one diagnosis. In contrast, 69.9% of the 113 ankylosing spondylitis patients received at least one temporomandibular dysfunction diagnosis, and only 30.1% were not included in any diagnosis group (p<0.001). Joint (p=0.001) and pain disorders (p=0.008) were significantly more common in the ankylosing spondylitis group than in the healthy controls. Significant associations emerged between Bath Ankylosing Spondylitis Disease Activity Index (p<0.001) and Bath Ankylosing Spondylitis Functional Index (p=0.005) scores and pain disorders. CONCLUSION: Temporomandibular dysfunction is more prevalent in ankylosing spondylitis patients than in healthy individuals, linked to increased joint issues and pain associated with disease activity. ClinicalTrials.gov ID: NCT05839925. OBJECTIVE controls factors METHODS 11 1875 18 75 18–75 I RESULTS 609 60 9 60.9 391 39 1 39.1 contrast 699 69 69.9 301 30 30.1 p<0.001. p0001 p p<0.001 . 0 001 p=0.001 (p=0.001 p=0.008 p0008 008 (p=0.008 (p<0.001 p=0.005 p0005 005 (p=0.005 CONCLUSION ClinicalTrialsgov ClinicalTrials gov ID NCT05839925 NCT 187 7 18–7 6 60. 3 39. 69. 30. p000 p<0.00 00 p=0.00 (p=0.00 (p<0.00 NCT0583992 18– p00 p<0.0 p=0.0 (p=0.0 (p<0.0 NCT058399 p0 p<0. p=0. (p=0. (p<0. NCT05839 p<0 p=0 (p=0 (p<0 NCT0583 p< p= (p= (p< NCT058 (p NCT05 NCT0

ABSTRACT Agriculture requires new alternatives to control pests and diseases; biological controls can be a sustainable alternative for the continued success of this sector. This study was thus carried out to identify yeasts and lactic acid bacteria that have the antagonistic capacity to control three phytopathogens as Botrytis cinerea, Lasiodiplodia theobromae and Alternaria brassicae that have caused significant economic losses in agriculture. Thirteen strains of yeast and seven strains of lactic acid bacteria (LAB) were used to measure the percentage of growth inhibition, production of volatile organic compounds, production of biofilms, and the production of enzymes through a completely randomized design. The yeasts Hanseniaspora opuntiae and Saccharomyces cerevisiae and the LAB Lactococcus lactis and L. brevis stood out for their antagonistic capacity to inhibit the growth of phytopathogens by 66%, 58%, 65% and 39%, respectively. Pest and disease control highly depend on chemical phytosanitary inputs with negative economic, environmental, and social effects. This study demonstrated that the yeast H. opuntiae and the LAB L. brevis have potential as biological controls and has been observed to inhibit growth by more than 39%, providing a sustainable alternative that is less harmful to the environment and human health. To guarantee their effectivity under field conditions, their individual application, consortium application, and concentration, timing, and proper application method must be considered.

SUMMARY OBJECTIVE: Coronary artery disease is the leading cause of death worldwide. Vascular endothelial growth factor is known to induce endothelial cell migration and proliferation, increase vascular permeability, and modulate thrombogenicity. The aim of this study is to investigate the relationship between the VEGF insertion/deletion (I/D) variant (rs35569394) and coronary artery disease susceptibility in the Turkish population. METHODS: A total of 206 subjects, including 106 coronary artery disease patients and 100 controls, were included in this study. The VEGF I/D variant was genotyped using the polymerase chain reaction method. RESULTS: The frequency of the I/I, I/D, and D/D genotypes was 35.84 versus 37%, 33.97 versus 36%, and 30.19 versus 27% in patients and the control group, respectively. VEGF I/D genotype and allele distribution were not statistically significant between coronary artery disease patients and controls (p>0.05). There was no significant difference between VEGF I/D genotype distribution and patient characteristics including age, gender, disease duration, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglyceride, history of hypertension, history of diabetes mellitus, and smoking (p>0.05). CONCLUSION: This study suggests that the VEGF I/D variant is not a predisposing factor to coronary artery disease disease in a Turkish sample. OBJECTIVE worldwide proliferation permeability thrombogenicity insertiondeletion insertion deletion ID I D (I/D rs35569394 rs (rs35569394 population METHODS 20 subjects 10 method RESULTS II I/I DD 3584 35 84 35.8 37 37% 3397 33 97 33.9 36 36% 3019 30 19 30.1 27 group respectively p>0.05. p005 p p>0.05 . 0 05 (p>0.05) age gender duration highdensity high density cholesterol lowdensity low triglyceride hypertension mellitus CONCLUSION sample rs3556939 (rs3556939 2 1 358 3 8 35. 339 9 33. 301 30. p00 p>0.0 (p>0.05 rs355693 (rs355693 p0 p>0. (p>0.0 rs35569 (rs35569 p>0 (p>0. rs3556 (rs3556 p> (p>0 rs355 (rs355 (p> rs35 (rs35 (p rs3 (rs3 (rs

Abstract Introduction: External cephalic version (ECV) allows to reduce the rate of cesarean sections due to breech presentation, with a variable success rate and without increasing obstetric complications, although it has been described that after successful ECV there could be a higher rate of intrapartum cesarean sec3ons as well as instrumental deliveries. Objective: To analyse results and factors associated with vaginal delivery after ECV, compared to delivery in patients with a spontaneously cephalic fetus. Method: Retrospective case-control study in patients with ECV performed in the same clinic. Each case was matched to a control. Demographic and clinical variables were analysed to determine maternal and neonatal outcomes as well as predictors of mode of delivery in successful ECV patients. Results: Four hundred fifty-eight ECV patients proposed were included, of which 276 (60.3%) were successful. Success was associated with multiparity, uterine relaxation and female fetal sex. Two hundred twenty-seven patients had a birth in our centre, who were matched with 229 controls. The ECV group had a higher rate of cesarean sections (23.3 vs. 15.3%; p = 0.03). Dystocia was the most frequent indication in both groups. Conclusions: ECV is a safe technique with a high success rate and good results, but once a successful version is achieved, the patient has a higher cesarean section rate attributable to any indication. Other obstetric were similar to patients with a spontaneously cephalic presentation fetus.

Resumen Introducción: La versión cefálica externa (VCE) permite disminuir la tasa de cesáreas por presentación de nalgas, con tasa de éxito variable y sin aumentar las complicaciones obstétricas, aunque se ha descrito que tras VCE exitosa podría haber mayor tasa de cesáreas intraparto así como de partos instrumentales. Objetivo: Analizar resultados y factores relacionados con el parto vaginal tras VCE exitosa comparándolos con el parto en pacientes con feto espontáneamente en cefálica. Método: Estudio retrospectivo de casos y controles en pacientes con VCE realizada en un único centro. Se analizaron variables demográficas y clínicas para determinar resultados maternos, neonatales y predictores de tipo de parto en las pacientes de VCE exitosa. Resultados: Se incluyeron 458 pacientes con VCE, de las cuales 276 (60,3%) fueron exitosas. El éxito se asoció a multiparidad, relajación uterina y sexo fetal femenino. Tuvieron un parto en nuestro centro 227 pacientes. Se emparejaron con 229 controles. El grupo VCE tuvo mayor tasa de cesáreas (23,3 vs. 15,3%; p = 0,03). La distocia fue la indicación más frecuente en ambos grupos. Conclusiones: La VCE es una técnica segura con alta tasa de éxito y buenos resultados. Una vez se consigue una versión exitosa la paciente tiene mayor tasa de cesárea atribuible a cualquier indicación, siendo los demás resultados obstétricos similares a las pacientes con un feto espontáneamente en presentación cefálica.