Abstract (Introduction): The search for techniques and methods for managing sludge generated during wastewater treatment is necessary to ensure sustainable sanitation in the medium term in Latin America. An interesting approach involves converting sludge into a biosolid usable in agriculture through alkaline sanitization. (Objective): We evaluated this technique’s efficacy and economic and environmental viability for obtaining an agriculturally usable type A biosolid from municipal wastewater treatment plant (WWTP) sludge. (Methodology): To this end, the efficacy in reducing microbiological parameters was experimentally tested at the laboratory scale using quicklime, for anaerobic and aerobic WWTP sludge. The experimental results fed a benefit/cost analysis model and a life cycle assessment. (Results): It was confirmed that applying a 40 % lime dose allowed the obtention of a type A biosolid with non-detectable concentrations of pathogenic microorganisms, which remained in that condition for more than 30 days. Additionally, the benefit/cost and life cycle analyses concluded that this option would be more attractive than sludge disposal in a landfill, due to the corresponding savings in chemical compounds (fertilizers, agricultural lime). (Conclusions): Therefore, alkaline sanitization can be considered suitable for WWTP sludge management. Its application on a larger scale should be further investigated in the country and the Latin-American region, considering the risk associated to the presence of heavy metals too.

Resumen (Introducción): La búsqueda de técnicas y modelos de gestión del lodo generado durante el tratamiento de las aguas residuales es necesaria para garantizar un saneamiento sostenible a mediano plazo en América Latina. Una vía interesante consiste en convertir el lodo en un biosólido aprovechable en la agricultura por medio de la higienización alcalina. (Objetivo): Se evaluó la eficacia y la viabilidad económica y ambiental de esta técnica para la obtención de un biosólido tipo A aprovechable en la agricultura a partir de lodo de planta de tratamiento de aguas residuales (PTAR) ordinaria. (Metodología): Para ello se probó experimentalmente en escala de laboratorio la eficacia en el decaimiento de parámetros microbiológicos higienizados con cal viva, usando lodo anaeróbico y aeróbico de PTAR. Los resultados experimentales alimentaron un modelo de análisis de beneficio/costo y de ciclo de vida. (Resultados): Pudo corroborarse que la aplicación de dosis de cal de 40 % permitió obtener un biosólido tipo A con concentraciones de microorganismos patógenos no detectables, lo cual se mantuvo en esa condición por más de 30 días. Además, el análisis de beneficio/costo y de ciclo de vida permitieron concluir que esta opción sería más atractiva que la disposición del lodo en un relleno sanitario, debido al ahorro correspondiente en compuestos químicos (fertilizantes o cal agrícola) derivado de su aprovechamiento. (Conclusiones): Por lo tanto, la técnica de higienización alcalina puede considerarse como adecuada para la gestión del lodo de PTAR y su aplicación a mayor escala debe investigarse en mayor detalle en el país y la región latinoamericana, considerando también el riesgo de presencia de metales pesados en este subproducto.

The present work focused on the screening process of different inorganic oxides and different temperatures for the epoxidation reaction of macaw palm vegetable oil without using solvent, a promising Brazilian biomass that can substitute other vegetable oils (e.g., soybean oil) in technological applications. This oil has an iodine value of 108.48 g of I2 per 100 g being categorized as a semi-dry oil. The epoxidized vegetable oil can be applied in manifold areas in polymer science. The epoxidation reaction here presented avoided organic solvents and organic acids and aimed to produce a greener route using metal oxides or niobium phosphate as a peroxide-activating catalyst. The experimental parameters were maintained as 10 mol% of catalyst loading and a time of 24 h; however, the temperature for some catalysts was changed to improve the epoxide conversion. Experiments were measured by proton nuclear magnetic resonance (1H NMR). Experiments using titanium oxide (TiO2) as a catalyst reached a conversion greater than 84.7%, while experiments using aluminium oxide (Al2O3) or lanthanum oxide (La2O3) achieved conversions of 22.6 and 30.1%, respectively. Furthermore, it was observed that both niobium compounds instantly react with hydrogen peroxide to form peroxy derivatives. solvent e.g., eg e (e.g. applications 10848 108 48 108.4 I semidry semi dry science peroxideactivating activating 1 mol 2 h however 1H H NMR. NMR . NMR) TiO2 TiO (TiO2 847 84 7 84.7% Al2O3 AlO Al O (Al2O3 La2O3 LaO La (La2O3 226 22 6 22. 301 30 30.1% respectively Furthermore derivatives e.g. (e.g 1084 4 108. (TiO 8 84.7 Al2O (Al2O La2O (La2O 3 30.1 e.g 84. 30.

This study aims to propose a new method for derivatizing fatty acids (FAs) from human milk (HM), eliminating the lipid extraction step, and simplifying the preparation for gas chromatography with a flame ionization detection (GC-FID) analysis to quantify the FAs. The Design Expert software optimized the reaction times, concentrations, and sample amount. The proposed method (PM) was validated for lyophilized HM and results for the figures of merit for precision in relative standard deviation (RSD) (RSDintra-day 1.34-4.03% and RSDinter day 2.08 5.16%), accuracy (99.87-102.16%), and robustness are within a linear range of 3 to 38% lipids in HM samples. The atmospheric solids analysis probe tandem mass spectrometry (ASAP-MS/MS) technique confirmed the efficiency of PM by expressing the molecular composition of triacylglycerol formed by FAs from the GC-FID technique. The PM requires a small sample size and conducts derivatization directly in the sample matrix, minimizing extraction errors. (FAs HM, , (HM) step GCFID GC FID (GC-FID times concentrations amount (PM RSD (RSD RSDintraday RSDintra 1.344.03% 134403 1.34 4.03% 1 34 4 03 1.34-4.03 208 2 08 2.0 5.16%, 516 5.16% 5 16 5.16%) 99.87102.16%, 998710216 99.87 102.16% 99 87 102 (99.87-102.16%) 38 samples ASAPMS/MS ASAPMSMS ASAP MS/MS MS (ASAP-MS/MS matrix errors (HM 344 1.344.03 13440 134 1.3 403 4.03 0 1.34-4.0 20 2. 51 5.16 87102 99.87102.16% 99871021 9987 99.8 10216 102.16 9 8 10 (99.87-102.16% ASAPMS MSMS 1.344.0 1344 13 1. 40 4.0 1.34-4. 5.1 8710 99.87102.16 9987102 998 99. 1021 102.1 (99.87-102.16 1.344. 4. 1.34-4 5. 871 99.87102.1 998710 102. (99.87-102.1 1.344 1.34- 99.87102. 99871 (99.87-102. 99.87102 (99.87-102 99.8710 (99.87-10 99.871 (99.87-1 (99.87- (99.87 (99.8 (99. (99 (9 (

Gold and platinum bimetallic nanoparticles, Au@PtNPs, have gained attention due to their plasmonic properties. The controlled synthesis of Au@PtNPs involves complex parameters, and the resulting synergy between Au and Pt introduces possibilities for tailoring their properties. This study explores the effects of key-parameters of synthesis (gold core diameters, reaction time, and Pt concentration) on the final properties of Au@PtNPs. Gold nanoparticles of varying diameters were synthesized and used as cores for the reduction of hexachloroplatinic acid, forming a surrounding shell of Pt nanoparticles. The parameters were systematically tuned to understand their impact on morphology, dimension, stability, and plasmonic properties of Au@PtNPs. Increasing Pt precursor concentration resulted in thicker and denser Pt shells, broadening the localized surface plasmon resonance (LSPR) band and improving Au@PtNPs stability. These properties are essential for applications such as photothermal treatment. The synthesis achieved satisfactory products within just 1 h of Pt4+ reduction at mild temperature without using surfactants. Only limited changes in plasmonic properties were observed after 4 h of synthesis, suggesting an optimal reaction period. Manipulating Au core diameter provided LSPR band control, with smaller cores exhibiting greater broadening towards the infrared region. This systematic exploration provided valuable insights for understanding key-parameters governing the synthesis and properties of Au@PtNPs. AuPtNPs PtNPs keyparameters key gold time acid morphology dimension stability shells (LSPR treatment Pt4 surfactants period control region

Parthenolide (PTN) is a secondary metabolite of the plant Tanacetum parthenium (L.) Schulz Bip. and is considered the chemical marker of this species. This sesquiterpene lactone (germacrene skeleton) has been described as responsible for the biological activity of the leaf extract. In addition, several studies in the literature have demonstrated its antiparasitic and antineoplastic activity. However, there is a need for knowledge of other safety parameters, such as pharmacokinetic, pharmacodynamic and toxicity evaluations. Therefore, in this investigation, the in vitro metabolism of parthenolide was performed with rat liver microsomes and biomimetic metabolism (such as cytochrome P-450 system) using organometallic catalysts. The biomimetic procedure was validated since the major compound of biomimetic oxidative reaction with meta-chloroperbenzoic acid catalyzed by metalloporphyrin 5,10,15,20-tetrakis(pentafluorophenyl)-porphyrin iron(III) chloride was also observed with rat liver microsomes. Previous chemoenzymatic synthesis studies of PTN afforded the same epoxide formation, this major oxidative compound was isolated and fully characterized as (1 R,10R)-epoxyparthenolide based on experimental and theoretical calculations of infrared (IR) and vibrational circular dichroism (VCD) data at the B3PW91/6-311G. Furthermore, for the first time, this metabolite was evaluated for trypanocidal and leishmanicidal activity. The mean inhibitory concentration (IC50) values were lower for PTN than its metabolite and showed significant cytotoxic effects for both parasites. This finding holds promise for addressing neglected diseases. (PTN L. L (L. Bip species germacrene skeleton extract addition However parameters pharmacokinetic evaluations Therefore investigation P450 P 450 P-45 system catalysts metachloroperbenzoic meta chloroperbenzoic 5,10,15,20tetrakispentafluorophenylporphyrin 5101520tetrakispentafluorophenylporphyrin tetrakispentafluorophenylporphyrin 5,10,15,20 tetrakis pentafluorophenyl porphyrin 5 10 15 20 ironIII iron III iron(III formation 1 ( R,10Repoxyparthenolide R10Repoxyparthenolide RRepoxyparthenolide R,10R epoxyparthenolide R 10R IR (IR VCD (VCD B3PW91/6311G. B3PW916311G BPWG B3PW91/6 311G. B3PW91 6 311G B PW G B3PW91/6-311G Furthermore time IC50 IC (IC50 parasites diseases (L P45 45 P-4 20tetrakispentafluorophenylporphyrin 5101520 5,10,15,2 2 10Repoxyparthenolide Repoxyparthenolide R10R RR 6311G B3PW91/6311G B3PW916 BPW B3PW91/ B3PW9 IC5 (IC5 P4 4 P- 510152 5,10,15, B3PW (IC 51015 5,10,15 5101 5,10,1 510 5,10, 51 5,10 5,1 5,

Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by extracellular accumulation of amyloid-β (Aβ) peptides in the brain. This study demonstrates the direct association of the disposable pipette extraction (DPX) with tandem mass spectrometry (MS/MS) for the analysis of Aβ peptides in cerebrospinal fluid (CSF) samples. Different parameters were optimized in order to improve detectability in the MS/MS including mobile phase percentage of ammonium hydroxide, mobile phase flow rate and acquisition mode. Also, this method used an electrospray ionization (ESI) low-flow probe and direct infusion of an organic solution in the MS/MS. The DPX-MS/MS method showed adequate linearity for determining Aβ peptides in CSF-linearity ranged from 0.1 to 1.5 ng mL 1. The coefficients of determination were higher than 0.99; the precision coefficient of variation (CV) ranged from 0.3 to 12.7%; and the accuracy relative standard deviation (RSD) ranged from -13.6 to 13.2%. Alzheimers Alzheimer s AD (AD amyloidβ amyloid β (Aβ brain DPX (DPX MSMS MS (MS/MS CSF (CSF samples hydroxide mode Also ESI (ESI lowflow low DPXMS/MS DPXMSMS CSFlinearity 01 0 1 0. 15 5 0.99 099 99 CV (CV 03 3 12.7% 127 12 7 RSD (RSD 13.6 136 13 6 -13. 132 2 13.2% DPXMS 0.9 09 9 12.7 13. -13 13.2 12. -1 -

APPLICATION OF MASS SPECTROMETRY WITH PAPER SPRAY IONIZATION AND THE OPLS-DA CHEMOMETRIC METHOD FOR THE DIAGNOSIS OF PROSTATE CANCER BY ANALYSIS OF URINE SAMPLES. In this study, paper spray ionization mass spectrometry (PSI-MS) allied to orthogonal projections on the latent structure-discrimination analysis (OPLS-DA) was employed to differentiate urine samples from patients with prostate cancer and control individuals (without prostate cancer). The parameters of sensitivity, specificity, efficiency and Matthew’s correlation coefficient determined to analyze the efficiency and quality of the OPLS-DA model obtained a value equal to 1, which means, respectively, that the model has a good performance to correctly classify true positive samples and true negatives. These results showed 100% correct classification of the samples from the training and test set, demonstrating a high efficiency of the OPLS-DA model when classifying all samples considered as belonging to the class of interest (urine from control patient) into class 1 and all samples considered as belonging to the non-interest class (urine from patients with prostate cancer) in class 0. This efficiency resulting from the application of this model suggests that the PSI-MS combined to OPLS-DA is a promisor approach for a rapid diagnosis of prostate cancer, via chemical fingerprints of urine samples. OPLSDA OPLS DA SAMPLES study PSIMS PSI MS (PSI-MS structurediscrimination structure discrimination (OPLS-DA without cancer. . sensitivity specificity Matthews Matthew s means respectively negatives 100 set patient noninterest non 0 10

Computational predictive approaches present themselves as an attractive alternative in the search for new compounds that may present toxic or growth regulatory activity in Aedes aegypti. Aspergillus species is a producer of secondary metabolites with diverse biological activities. In this study, it was possible to evaluate the pharmacokinetic and toxicological properties in silico, as well as the prediction of the insecticidal biological activities of the juvenile hormone of natural substances produced by the genus Aspergillus. Initially, 187 molecules were cataloged from various species of Aspergillus spp., and only seven molecules of indole alkaloids were found to exhibit a toxic dose 50% (TD50) after conducting toxicity predictions. The molecular dynamic simulation trajectories were utilized to study parameters such as root mean square deviation, root mean square fluctuation, radius of gyration and intermolecular hydrogen bond to understand the behavior and stability of proposed compounds in the binding pocket of the target protein. The N-β-acetyltryptamine molecule showed a binding affinity value of –8.100 ± 0.200 kcal mol-1, which classifies the compound as a potential insecticidal agent against the Aedes aegypti vector. silico Initially 18 spp spp. 50 TD50 TD (TD50 predictions deviation fluctuation protein Nβacetyltryptamine N β acetyltryptamine 8100 8 100 –8.10 0200 0 200 0.20 mol1, mol1 mol 1, 1 mol-1 vector 5 TD5 (TD5 810 10 –8.1 020 20 0.2 mol- (TD 81 –8. 02 2 0. –8 –

This research aims to develop a precise and efficient dispersive solid-phase microextraction (DSPE) technique using third-generation polyamidoamine (PAMAM) dendrimer-grafted nanoparticles to quantify benzoylurea pesticides in green tea, black tea, and oolong tea infusions. The extraction efficiency is optimized through single-factor adjustments of key parameters, including adsorbent quantity (5-20 mg), adsorption duration (5-20 min), desorbent identity (methanol, acetonitrile), volume (100-600 μL), sodium chloride concentration (0-10%, m/v), and sample solution pH (3-8). The optimized method demonstrates linearity in the range of 2-500 μg L-1, with limits of detection (LODs) ranging from 0.6-1.5 μg L-1 and limits of quantification (LOQs) from 2.0 5.0 μg L-1. Recovery rates of 81.2-96.0% are achieved, with intra-day relative standard deviations (RSDs) of 1.4-2.8% and inter-day relative standard deviations of 2.0-3.9%, indicating high accuracy and repeatability. The successful application of detecting residues in commercial tea samples underscores the strong potential of the technique to determine benzoylurea analyte levels. solidphase solid phase DSPE (DSPE thirdgeneration third generation PAMAM (PAMAM dendrimergrafted dendrimer grafted infusions singlefactor single factor parameters 520 5 20 (5-2 mg, mg , mg) min, min min) methanol, methanol (methanol acetonitrile, acetonitrile acetonitrile) 100600 100 600 (100-60 μL, μL μL) 010%, 010 0 10%, 10 (0-10% m/v, mv m/v m v m/v) 38. 38 3 8 . (3-8) 2500 2 500 2-50 L1, L1 L 1, 1 LODs (LODs 0.61.5 0615 0.6 1.5 6 0.6-1. L- LOQs (LOQs 2. 50 5. L1. 1. 81.296.0% 812960 81.2 96.0% 81 96 81.2-96.0 achieved intraday intra day RSDs (RSDs 1.42.8% 1428 1.4 2.8% 4 1.4-2.8 interday inter 2.03.9%, 2039 3.9%, 9 2.0-3.9% repeatability levels 52 (5- 10060 60 (100-6 010% 01 10% (0-10 (3-8 250 2-5 61 0.61. 061 06 0. 15 0.6-1 296 81.296.0 81296 812 81. 960 96.0 81.2-96. 42 1.42.8 142 14 28 2.8 1.4-2. 03 2.03.9% 203 39 3.9% 2.0-3.9 (5 1006 (100- (0-1 (3- 25 2- 0.61 0.6- 29 81.296. 8129 96. 81.2-96 1.42. 1.4-2 2.03.9 3.9 2.0-3. ( (100 (0- (3 81.296 81.2-9 1.42 1.4- 2.03. 3. 2.0-3 (10 (0 81.29 81.2- 2.03 2.0- (1

The in silico study aimed to suggest phytochemicals from Commiphora leptophloeos leaves essential oil as therapies against coronavirus disease (COVID-19) (or severe acute respiratory syndrome coronavirus 2, SARS-CoV-2). For that, we used molecular docking (MD), absorption, distribution, metabolism, excretion, and toxicity (ADMET) parameters, and pharmacokinetic analysis. MD showed the presence of hydrophobic and hydrogen bonds for nine compounds. β-Selinene and bicyclogermacrene showed the best interaction values with the protein Mpro (ID: 6Y2F) (-5.9) and chimeric receptor complexed with its receptor human ACE2 (ID: 6VW1) (-6.1 and -5.5) proteins, as well as low gastrointestinal and high epithelial absorptions, medium permeability for Caco-2 cells and high epithelial permeability, low oral excretion, and were inhibitors of CYP2C19 and CYP2C9 enzymes. On the other hand, both were not permeable to the blood-brain barrier and inhibitors or substrates for glycoprotein-D, and showed carcinogenic potential, but only β-selinene was considered mutagenic. Although little bioavailable, both presented aspects structurally adequate, being compounds with moderate synthesis difficulty. Therefore, more analyses are necessary to evaluate the mechanism of action and unsatisfactory parameters foreseen in bioinformatic assays. COVID19 COVID 19 (COVID-19 2 SARSCoV2. SARSCoV2 SARSCoV SARS CoV . SARS-CoV-2) that MD, , (MD) absorption distribution metabolism excretion ADMET (ADMET analysis βSelinene β Selinene ID (ID 6Y2F YF Y F 5.9 59 5 9 (-5.9 ACE 6VW1 VW 6.1 61 6 1 (-6. 5.5 55 -5.5 proteins absorptions Caco2 Caco Caco- CYPC CYP C CYP2C1 CYP2C enzymes hand bloodbrain blood brain glycoproteinD, glycoproteinD glycoprotein D, D glycoprotein-D potential βselinene selinene mutagenic bioavailable adequate difficulty Therefore assays COVID1 (COVID-1 SARS-CoV-2 (MD 5. (-5. 6VW 6. (-6 -5. (COVID- SARS-CoV- (-5 (- -5 (COVID SARS-CoV ( -

Caffeine (CAF) and nicotine (NIC) are emerging contaminants and are among the most consumed substances in the world, making it crucial to monitor these contaminants. In this work, an alternative electrolyte, 2-hydroxyethylammonium acetate (2HEAA) was used for the development of an electroanalytical method for determination and quantification of CAF and NIC, simultaneously, using differential pulse voltammetry (DPV). The system with the 2HEAA electrolyte was characterized by cyclic voltammetry and electrochemical impedance spectroscopy, and the DPV parameters were optimized for the best conditions. The method was validated from a calibration curve obtained which showed limit of detection (LOD) of 0.82 and 6.26 μmol L 1 and limit of quantification (LOQ) of 2.73 and 20.8 μmol L-1 for CAF and NIC, respectively. In the precision analyses, values lower than 10% of relative standard deviation were obtained. In the presence of concomitant inorganic and organic species, the system proved to be selective in the determination of analytes. The method was used to determine the analytes in fortified samples (river water, synthetic urine and commercial milk), obtaining recoveries between 87.25 and 111.40%. The 2HEAA demonstrated high efficiency as an alternative electrolyte with good signal-to-noise ratio, increased analytical sensitivity of the method, in addition to presenting low cost and fast electrolyte preparation. (CAF NIC (NIC world work 2hydroxyethylammonium hydroxyethylammonium 2 HEAA (2HEAA simultaneously DPV. . (DPV) spectroscopy conditions LOD (LOD 082 0 82 0.8 626 6 26 6.2 LOQ (LOQ 273 73 2.7 208 20 8 20. L1 L- respectively analyses 10 species river water milk, milk , milk) 8725 87 25 87.2 11140 111 40 111.40% signaltonoise signal noise ratio preparation (DPV 08 0. 62 6. 27 7 2. 872 87. 1114 11 4 111.40 111.4 111.

Groundwater is considered an important source of water aid for human consumption. However, this water can be contaminated by impurities, such contamination spots that might reach the water tables. The aim of this study was to investigate the quality of shallow groundwater in Flores neighborhood, located in the municipality of Benevides, within Belém metropolitan region, State of Pará, Brazil. Recommendations described in Standard Methods for Examination of Water and Wastewater were followed at the time to determine physical-chemical parameters such as potential hydrogen (pH), electrical conductivity (EC), total dissolved solids (TDS), apparent color, turbidity, total hardness (TH), ions and trace elements. Multivariate statistical analysis has shown that most predictive water-quality parameters set for human consumption are associated with natural events involving weathering and leaching processes. Nevertheless, parameters of nitrate, phosphate, EC, TDS and pH are also influenced by anthropic conditions in the surroundings, mainly phosphate and nitrate ions. Therefore, it can be concluded that shallow groundwaters in Flores neighborhood are unsuitable for human consumption, making it necessary for municipal public authorities to take action to solve the problem of water shortage in the borough, thus preventing the population from use those shallow groundwaters, as their consumption can bring health problems. However impurities tables Benevides region Pará Brazil physicalchemical physical chemical pH, , (pH) EC (EC) TDS, (TDS) color turbidity TH, TH (TH) elements waterquality processes Nevertheless surroundings Therefore borough problems (pH (EC (TDS (TH

Electrooxidation is an efficient method for treating effluents with high concentrations of ammoniacal nitrogen and organic matter (R), in the presence of chloride ions (Cl-). The present study aimed at degrading ammoniacal nitrogen and organic matter from an effluent generated at a fertilizer storage port terminal. For this purpose, a batch electrochemical reactor with mechanical agitation was used, with a Ti/RuO2 anode. The parameters studied were the current density that varied in 20, 40 and 60 mA cm-2, the concentration of chloride ions that was 0.04, 0.74, 0.90 to 0.81 mol L-1 and the electrolysis time that varied from 4.0, 5.0, 7.0 and 8.0 h. A 22 experimental design was conducted to determine the significance level of the parameters studied. The results obtained indicated the removal of 99.99% of N-NH3 and 75.60% of total organic carbon (TOC) when a current density of 60 mA cm-2 and a Cl- concentration of 35.5 g L-1 were applied, after 4.0 h of electrolysis. R, R , (R) Cl. Cl . (Cl-) terminal purpose used TiRuO2 TiRuO Ti RuO2 RuO Ti/RuO anode 20 4 6 cm2, cm2 cm 2, 2 004 0 04 0.04 074 74 0.74 090 90 0.9 081 81 0.8 L1 L 1 L- 50 5 5.0 70 7 7. 80 8 8. 9999 99 99.99 NNH3 NNH N NH3 NH N-NH 7560 75 75.60 TOC (TOC cm- 355 35 35. applied 4. (R (Cl- 00 0.0 07 0.7 09 9 0. 08 5. 999 99.9 756 75.6 3 (Cl 99. 75.

RESUMO: O melão de polpa alaranjada honeydew apresenta elevado potencial nutricional e econômico, sendo amplamente apreciado como fruta doce e refrescante. Portanto, avaliar amostras comerciais de produtos tão relevantes para o mercado de frutas frescas é de grande importância. O objetivo deste estudo foi investigar a capacidade antioxidante, os parâmetros de qualidade e a composição química de melões do mercado brasileiro. Os valores de pH e ºBrix determinados para as amostras de melão foram 6,5 ± 0,30 e 9,2 ± 0,80 g/100 g (peso fresco), respectivamente, o que está de acordo com os resultados esperados para melões de alta qualidade. A análise cromatográfica revelou a sacarose (49% do açúcar total) como principal açúcar, seguida pela frutose (30%) e glicose (21%). Os carotenóides, que estão diretamente ligados à cor laranja característica, apresentaram teor total de 270,59 µg/g (peso seco). Além disso, o maior valor de b* comparado ao valor de a* indica a maior contribuição da cor amarela. Quanto ao potencial antioxidante, além de apresentar capacidade antioxidante para ambos os métodos aplicados, DPPH e FRAP, níveis variando de 16,40 ± 0,21 µmol equivalente de ácido ascórbico/g a 56,98 ± 0,80 µmol equivalente de Trolox/g (peso seco) foram encontrados nos extratos de melão. Ademais, os resultados foram maiores em termos de FRAP do que em DPPH. Desta forma, os resultados destacam os melões avaliados como fontes valiosas de compostos bioativos, como carotenóides e fenólicos, que contribuem para suas propriedades antioxidantes. Além disso, análises de cor, pH e teor de sólidos solúveis totais também refletem os atributos de qualidade. RESUMO econômico refrescante Portanto importância brasileiro 65 6 5 6, 030 0 30 0,3 92 9 2 9, 080 80 0,8 g100 100 g/10 peso fresco, fresco , fresco) respectivamente 49% 49 (49 30% (30% 21%. 21 21% . (21%) característica 27059 270 59 270,5 µgg µg seco. seco disso b amarela aplicados 1640 16 40 16,4 021 0,2 ascórbicog ascórbico 5698 56 98 56,9 Troloxg Trolox Ademais forma bioativos fenólicos antioxidantes 03 3 0, 08 8 g10 10 g/1 4 (4 (30 (21% 2705 27 270, 164 1 16, 02 569 56, g1 g/ ( (3 (21 (2

ABSTRACT: Orange-fleshed non-netted honeydew melon has high nutritional and economic potential, being widely appreciated as a refreshing sweet fruit. Therefore, evaluating commercial samples of such relevant products for the fresh fruit market is of great importance. This study investigated the antioxidant capacity, quality parameters, and chemical composition of honeydew melons from the Brazilian market. The values of pH and °Brix determined for melon samples were 6.5 ± 0.30 and 9.2 ± 0.80 g/100 g (fresh weight), respectively, which agree with the results expected for high-quality melons. The chromatography analysis revealed sucrose (49% of total sugar) as the main sugar, followed by fructose (30%) and glucose (21%). Carotenoids, which are directly linked to the characteristic orange color, showed a total level of 270.59 µg/g (dry weight). Additionally, the higher b* value compared to a* value indicates the highest yellow contribution. Regarding the antioxidant assays, in addition to presenting antioxidant capacity for both methods applied, DPPH and FRAP, levels ranging from 16.40 ± 0.21 µmol ascorbic acid equivalent/g to 56.98 ± 0.80 µmol Trolox equivalent/g (dry weight) were reported in melon extracts. Moreover, the results were higher in terms of FRAP than DPPH. Therefore, the results underscore the evaluated honeydew melons as valuable sources of bioactive compounds, such as carotenoids and phenolics, which contribute to their antioxidant properties. Besides, analyses of color, pH, and total soluble solids content also reflect the quality attributes. ABSTRACT Orangefleshed Orange fleshed nonnetted non netted potential Therefore importance parameters Brix 65 6 5 6. 030 0 30 0.3 92 9 2 9. 080 80 0.8 g100 100 g/10 weight, weight , respectively highquality 49% 49 (49 sugar 30% (30% 21%. 21 21% . (21%) Carotenoids color 27059 270 59 270.5 µgg µg dry weight. Additionally b contribution assays applied 1640 16 40 16.4 021 0.2 equivalentg equivalent 5698 56 98 56.9 extracts Moreover compounds phenolics properties Besides attributes 03 3 0. 08 8 g10 10 g/1 4 (4 (30 (21% 2705 27 270. 164 1 16. 02 569 56. g1 g/ ( (3 (21 (2

Resumen Ha sido la permanente preocupación de los ingenieros de empresas municipales en el país, respecto a la determinación de las velocidades máximas y los problemas relacionados por abrasión en las tuberías plásticas, parámetros que pueden ocasionar la pérdida de espesor de pared en las tuberías de alcantarillado sanitario y pluvial. Este artículo establece sobre la investigación obtenida en ensayos de laboratorio en tuberías de PRFV (poliéster reforzado en fibra de vidrio) que con nuevas innovaciones y formulaciones químicas (carburo de silicio), es actualmente, más resistente al fenómeno de la abrasión y por ende a la velocidad del fluido. Muestra que, aunque los métodos de las pruebas de abrasión pueden variar, los resultados obtenidos en los ensayos realizados son consistentes en laboratorio y constatados en campo.

Abstract It has been the permanent concern of Municipal Company engineers in the country, regarding the determination of maximum speeds and the problems related to abrasion in plastic pipes. Parameters that can cause the loss of wall thickness in sanitary and storm sewer pipes. This article establishes the research obtained in laboratory tests on GRP pipes (fiberglass reinforced polyester) that with new innovations and chemical formulations (silicon carbide), is currently more resistant to the phenomenon of abrasion and therefore to the velocity of the fluid. It shows that, although abrasion test methods may vary, the results obtained in the tests carried out are consistent in the laboratory and verified in the field.