Cupuaçu (Theobroma grandiflorum Schum.) is an Amazonian fruit rich in antioxidants potential due to its phenolic compounds. Its seeds produce cupulate, a chocolate-like product, and the residue remains rich in biocompounds. This study aimed to optimize and compare microwave-assisted (MAE) and high pressure/temperature extraction (HPTE) of polyphenolic and flavonoid compounds from cupuaçu seed by-products. A Box-Behnken 33 factorial design assessed the influence of extraction time, solid/liquid ratio, and ethanol concentration. Responses included total polyphenol content, total flavonoid content, and anti-radical power via the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid) method. In optimum conditions, MAE had the highest total polyphenol content and HPTE for flavonoids. Optimal conditions for both methods were 65% (m/v) ethanol concentration, 45 min extraction time, and 0.03 g mL−1 solid/liquid ratio. MAE and HPTE achieved greater recovery of (−)-epicatechin and (−)-epigallocatechin-3-gallate compared to percolation. Both techniques proved viable for recovering polyphenols from cupuaçu seed by-products, in less time and with less solvent. Thus, cupuaçu by-products can be a sustainable and functional food source, with MAE and HPTE providing high yields and quality extracts rich in polyphenols. Theobroma Schum. Schum cupulate chocolatelike chocolate like product biocompounds microwaveassisted microwave assisted (MAE pressuretemperature pressure temperature (HPTE byproducts. byproducts by products. products BoxBehnken Box Behnken 3 solidliquid solid liquid ratio concentration antiradical anti radical 2,2azinobis3ethylbenzothiazoline6sulphonic 22azinobis3ethylbenzothiazoline6sulphonic azinobisethylbenzothiazolinesulphonic 2,2 azino bis ethylbenzothiazoline 6 sulphonic 2 acid method flavonoids 65 m/v mv m v (m/v 4 003 0 03 0.0 mL1 mL 1 mL− −epicatechin epicatechin − −epigallocatechin3gallate epigallocatechin3gallate epigallocatechingallate epigallocatechin gallate percolation byproducts, products, solvent Thus source 2azinobis3ethylbenzothiazoline6sulphonic azinobis 22 2, 00 0.

Nanoparticles of TiO2 doped with low amount of Fe were synthesized from natural ilmenite obtained from alluvial gold mine wastes. The obtained TiO2 were characterized using X-ray diffraction (XRD), X-ray fluorescence (XRF), Fourier-transform infrared spectroscopy (FTIR), UV-Vis diffuse reflectance, thermogravimetric analysis/differential scanning calorimetry (TGA/DSC), N2 adsorption-desorption isotherms, transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). The results confirm the formation of mesoporous TiO2 nanoparticles in anatase phase with 0.05 wt.% of iron possibly replacing some titanium places. All the characterizations were performed to the synthesized solid and the reference materials (TiO2 anatase and Degussa P25). The photocatalytic activity of both the synthesized solid and references were evaluated in the photo-oxidation of cyanide in aqueous medium under UV illumination. An experimental design type Box-Behnken was performed choosing three different parameters: the initial concentration of cyanide (50, 150 and 250 ppm) the catalyst load (0.1, 0.5 and 1.0 g L-1) and the oxygen source. The percentage of cyanide conversion was chosen as response in the design obtaining as optimal conditions [CN-]0 = 50 ppm, catalyst load = 0.6 g L-1 and air bubbling. Under these conditions it was reached 32, 32 and 89% of cyanide conversion for Fe-TiO2 synthesized, TiO2 anatase and Degussa P25, respectively. TiO wastes Xray X ray XRD, XRD , (XRD) XRF, XRF (XRF) Fouriertransform Fourier transform FTIR, FTIR (FTIR) UVVis Vis reflectance analysisdifferential analysis differential TGA/DSC, TGADSC TGA/DSC TGA DSC (TGA/DSC) N adsorptiondesorption adsorption desorption isotherms TEM (TEM XPS. XPS . (XPS) 005 0 05 0.0 wt wt. places (TiO P25. P25 P P25) photooxidation photo oxidation illumination BoxBehnken Box Behnken parameters 50, (50 15 25 ppm 0.1, 01 1 (0.1 5 0. 10 1. L1 L source CN0 CN [CN-] 06 6 L- bubbling 3 89 FeTiO2 FeTiO Fe-TiO respectively (XRD (XRF (FTIR (TGA/DSC (XPS 00 P2 (5 2 0.1 (0. [CN- 8 ( (0 [CN

Abstract Currently, food products, such as oil, are displayed at high lighting intensities and sometimes at temperatures above 20 °C, factors that can accelerate oxidative rancidity, such as degradation of oil quality. This is the reason why a PID (Proportional-Integral-Derivative) control system was implemented that allowed quality tests to be carried out on the oil stored in different temperature conditions (13 °C to 27 °C). During these tests, the transfer function of the system and the controller, was found using the automatic tuning of the control box. This improved the precision and continuous voltage for illumination (from 155 lux to 1145 lux) by varying the intensity of the LEDs. For the experimental design, the central composite design (CCD) was used, resulting in 13 treatments. After 30 days, the quality of the oil was evaluated (acidity index and peroxide index), and the oxidative stability index was determined using the Rancimat method. It was found that temperature and lighting have a significant effect on the quality and oxidative stability of the oil (p < 0.05), achieving the storage condition with greater stability at 300 lux of lighting and 15 °C temperature.

Resumen Actualmente, los productos alimenticios, como el aceite, se exhiben a elevadas intensidades de iluminación y en ocasiones a temperaturas por encima de los 20 °C, factores que pueden acelerar la rancidez oxidativa, como la degradación de la calidad del aceite. Esta es la razón por la cual se implementó un sistema de control PID (Proporcional-Integral-Derivativo) que permitió realizar pruebas de calidad del aceite almacenado en diferentes condiciones de temperatura (13 °C a 27 °C). Durante esas pruebas, se halló la función de transferencia del sistema y el controlador, utilizando la sintonía automática de la caja de control. Esto mejoró la precisión y el voltaje continuo para la iluminación (de 155 lux a 1145 lux), variando la intensidad de los LED. Para el diseño experimental, se utilizó el diseño central compuesto (DCC), lo que resultó en trece tratamientos. A los treinta días, se evaluó la calidad del aceite (índice de acidez e índice de peróxidos), y se determinó el índice de estabilidad oxidativa mediante el método Rancimat. Se encontró que la temperatura y la iluminación presentan un efecto significativo sobre la calidad y la estabilidad oxidativa del aceite (p < 0.05), logrando la condición de almacenamiento con mayor estabilidad a 300 lux de iluminación y 15 °C de temperatura.

Resumo Actuamente, os produtos alimenticios, como o óleo, são exibidos em elevadas intensidades de iluminação e em ocasiões em temperaturas por acima dos 20 °C, fatores que podem acelerar o ranço oxidativo, como a degradação da qualidade do óleo. Este é o motivo pelo qual implementou-se um sistema de controle PID (Proporcional-Integral-Derivativo) que permitiu realizar provas de qualidade do óleo almacenado em diferentes condições de temperatura (13 °C até 27 °C). Durante essas provas, encontrou-se a função de transferência do sistema e o controlador, utilizando a sintonia automática da caixa de controle. Isso melhorou a precisão e a tensão contínuo para a iluminação (de 155 lux até 1145 lux), variando a intensidad dos LED. Para o desenho experimental, utilizou-se o disenho central composto (DCC), o que resultou em treze tratamentos. Aos trinta días, avaliou-se a qualidade do óleo (índice de acidez e índice de peróxidos), e determinou-se o índice de estabilidade oxidativa mediante o método Rancimat. Encontrou-se que la temperatura e a iluminação apresentam um efeto significativo sobre a qualidade e a estabilidade oxidativa do óleo (p < 0.05), conseguindo a condição de almacenamento com maior estabilidade, em 300 lux de iluminação e 15 °C de temperatura.

ABSTRACT Introduction: This project aimed to recognize relational intelligence as an instrument of cohesion, satisfaction, and professional motivation with inherent repercussions on the quality and safety of care provided, as well as the promotion of healthy work environments. Diagnosis: Was use consensus research to identify the area of intervention, more specifically the “brainstorming” technique, prioritizing relational issues based on the number of times they were mentioned by employees. Objective: Promote relational intelligence in the team at the Surgery A unit, at Hospital Curry Cabral, as a management tool, increasing employee satisfaction in relation to the work environment by at least 20%. Methodology: The methodology used focused on two assessment moments, qualitative and quantitative. The qualitative assessment began with the project “Thinking outside the box…writing inside the box”, in which employees were encouraged to share emotions, suggestions, compliments, complaints, or any other subject they considered relevant. The anonymity of the contributions allowed sincerity and directness in the work environment to emerge without constraints, allowing psychological safety. This strategy was the “driver” of this project because, based on its contributions, it was possible to identify areas that could be improved. For a quantitative analysis, to measure the intended objective, was applied questionnaires on two evaluation moments. Results: With the contributions of the qualitative assessment, mas observed the need for intervention in terms of employees’ mental health. This began new routines, which include weekly reflection meetings, training on interpersonal relationships and health and well-being measures in the team. The strategies used allowed the results obtained in the quantitative evaluation with an increase of 44,1% in the satisfaction of this population. Conclusion: There is an association of the ability to actively listen to employees’ problems and their involvement. Participatory, transformative, and humanized leadership contributed to the well-being of professionals. If is therefore considered that this project added value in terms of improving the working environment, with repercussions on productivity and the quality of service provided to users.

RESUMO Introdução: Este projeto visa reconhecer a inteligência relacional como instrumento de coesão, satisfação e motivação profissional, com inerente repercussão na qualidade e segurança dos cuidados prestados, bem como na promoção de ambientes de trabalho saudáveis. Diagnóstico da situação: Procedeu-se ao diagnóstico da situação utilizando-se a pesquisa de consenso, mais especificamente a técnica de “brainstorming”, para identificar a área de intervenção, priorizando-se as questões relacionais pelo número de vezes que foram mencionadas pelos colaboradores. Objetivo: Promover a inteligência relacional na equipa da unidade Cirurgia A, do Hospital Curry Cabral, como instrumento de gestão, aumentando em pelo menos 20% a satisfação dos colaboradores, em relação ao ambiente de trabalho. Metodologia: A metodologia focou-se em dois momentos de avaliação, qualitativa e quantitativa. A primeira iniciou-se com o projeto “Pensar fora da caixa…escrevendo para dentro da caixa”- nesta os colaboradores foram estimulados a partilhar emoções, sugestões, elogios, reclamações ou qualquer outro assunto que considerassem pertinente. O anonimato dos contributos permitiu que a sinceridade e frontalidade sobre o ambiente de trabalho emergisse sem constrangimentos e em segurança psicológica. Esta estratégia foi, sem dúvida, o “motor” deste projeto pois, a partir dos seus contributos, foi possível identificar as áreas passiveis de melhoria. Para uma análise quantitativa, de forma que existissem indicadores para medir o objetivo pretendido, foram realizados dois momentos de avaliação através da aplicação de questionários. Resultados: Com os contributos da avaliação qualitativa observou-se a necessidade de intervenção a nível da saúde mental dos colaboradores. Deu-se assim inicio a novas rotinas, onde se incluíram reuniões semanais de reflexão, formação sobre relacionamento interpessoal e medidas de saúde e bem-estar na equipa. As estratégias utilizadas permitiram os resultados obtidos na avaliação quantitativa com um aumento de 44,1% na satisfação desta população. Conclusões: A capacidade de escuta ativa dos problemas dos colaboradores e o envolvimento dos mesmos na sua melhoria associado a uma liderança participativa, transformadora e humanizada contribuiu para o bem-estar dos profissionais. Considera-se assim que este projeto acrescentou valor no que diz respeito à melhoria do ambiente de trabalho, com repercussão na produtividade e na qualidade do atendimento feito aos utentes.

Resumo Este trabalho propõe uma aplicação web que usa um modelo de caixa preta Support Vector Machine (SVM) com 79% de acurácia para classificar o sentimento de tweets sobre a COVID-19 integrando o framework LIME de forma interativa para explicar decisões sobre previsões. Além do ganho de transparência em relação à avaliação de amostras falso-positivas, notou-se também que o modelo SVM tende a falhar ao associar um teste positivo de COVID-19 a um bom sentimento e se confunde em previso˜es envolvendo palavras sobre a COVID-19, como Omicron, que indica falta de representatividade na base de dados. Além disso, a partir dos resultados do LIME, foi possível melhorar a acura´cia do modelo para 81% ao incluir as stopwords ”not” e ”no”.

Abstract The present work proposes a web application that uses an Support Vector Machine (SVM) black box model with 79% accuracy to classify sentiment from tweets about COVID-19 integrating the LIME framework in an interactive way to explain decisions about predictions. Besides the gain in transparency in relation to the evaluation of false positive samples, it was also noted that the SVM model tends to fail when associating a positive COVID-19 test with a good sentiment and gets confused in specific predictions involving words related to COVID-19 variants such as Omicron, which indicate lack of representativeness in the database. In addition, from the LIME results, it was possible to improve the model accuracy to 81% by including the stopwords “not” and “no”.

Abstract Background: Verbesina molinaria B.L. Rob. & Greenm. and V. oncophora B.L. Rob. & Seaton constitute species with conflicting circumscription; different authors consider them as two different or just one species (V. oncophora). Questions: ¿Are they one or two species of Verbesina? ¿A linear discriminant analysis (LDA) will differentiate them as two species? Species studied/Mathematic model: Verbesina molinaria and V. oncophora / Royston, Shapiro-Wilk, Levene, correlation (Pearson), Variance inflation factor (VIF), and LDA. Study site and years: Mexico; 2022-2023. Methods: Types, protologues, as well as herbarium specimens at ENCB, HUMO, MEXU, and UAMIZ of the two species were analyzed, assigning a preliminary name to each one. A data matrix with the characters and character states was created to carry out the statistical analyses. Box plots were generated to evaluate differences among characters and the statistical tests were done using the software Rstudio. Results: Types and protologs analyses revealed morphological differences between both species. Royston and Shapiro-Wilk tests supported normality among characters evaluated, and the Levene test suggests there are no significant differences among them. Finally, Pearson correlation and the VIF values suggested the absence of correlation or collinearity. The LDA analysis showed 100 % accuracy in the assigning of specimens to the a priori species allocated. Conclusions: The two species represent distinct taxa based on the morphological differences and statistical support, one of them widespread in central Mexico (V. oncophora) and the other (V. molinaria) restricted as far as now to the states of Mexico, Morelos, and Michoacán.

Resumen Antecedentes: Verbesina molinaria B.L. Rob. & Greenm. y V. oncophora B.L. Rob. & Seaton son especies con circunscripción conflictiva; algunos autores las consideran como especies distintas mientras que otros como una sola (V. oncophora). Preguntas: ¿Son dos especies de Verbesina? ¿Un análisis linear discriminante (LDA) ayudará a diferenciarlas? Especies de estudio/Modelo matemático: Verbesina molinaria y V. oncophora / Pruebas de Royston, Shapiro-Wilk, Levene, correlación (Pearson), factor de inflación de la varianza (VIF) y LDA. Sitio y años de estudio: México; 2022-2023. Métodos: Se revisaron tipos, protólogos, así como ejemplares de ambas especies depositados en ENCB, HUMO, MEXU y UAMIZ, asignándoles un nombre preliminar y elaborando una matriz de datos. Se generaron diagramas de cajas para evaluar diferencias entre los caracteres analizados; las pruebas estadísticas se realizaron utilizando el software Rstudio. Resultados: Los tipos y protólogos permitieron identificar diferencias morfológicas entre ellas. Las pruebas de Royston y Shapiro-Wilk respaldaron la normalidad entre los caracteres evaluados. La prueba de Levene sugiere que no hay diferencias significativas entre los caracteres; finalmente, la correlación de Pearson y el VIF indican ausencia de correlación o colinealidad. El análisis LDA mostró una precisión del 100 % en la asignación de los ejemplares con las asignaciones a priori. Conclusiones: Las diferencias morfológicas y las pruebas estadísticas, apoyan el reconocimiento de dos entidades distintas, V. oncophora con amplia distribución en el centro de México y V. molinaria con distribución más restringida, mejor conocida hasta la fecha en los estados de México, Michoacán y Morelos.

ABSTRACT Introduction: According to Barros (2017), Bentonite is a mixture of clays, generally impure, very fine grained, formed mainly by 60 to 80% of montmorillonite and also Kaolinite, Calcite and Pyrite. At the operational base of Petromoc Maputo Company, there is a set of tanks for fuel storage, that from a complex of tanks with coupled pipelines for the entry and withdrawal of fuels and effluent disposal. Objective: To evaluate the use of bentonite in the removal of toxic cations from the effluents generated at Petromoc Company. Materials and Methods: Bentonite samples were collected at the Industrial Minerals company. The effluent and influent samples were collected in the separator box of the operational base. The physicochemical experiments were carried out at the Analytical Chemistry Laboratory of the Eduardo Mondlane University. For the physical characterization of the bentonite, the pycnometric density, porosity (P) and specific surface area were determined. The mineralogical characterization was performed by X-ray diffraction analysis. The chemical characterization of the effluent and influent was performed by Inductively Coupled Plasma Atomic Emission Spectroscopy. Results and Discussion: Mineralogical characterization showed the presence of bentonite minerals. The pH of the effluent before interacting with the bentonite was 3.22, after interaction the pH is between 5.8 and 9. High concentrations of Cadmium (Cd), Chromium (Cr), Copper (Cu), Titanium (Ti) and Zinc (Zn) were determined in the effluent before the interaction with the bentonite. After the interaction, a removal of toxic elements is verified. Conclusions: The bentonite showed high efficiency in the removal of toxic cations.

RESUMEN Introducción: Según Barros (2017), la Bentonita es una mezcla de arcillas, generalmente impuras, de grano muy fino, formada principalmente por 60 a 80% de montmorillonita y también Caolinita, Calcita y Pirita. En la base operacional de la Empresa Petromoc Maputo, existe un conjunto de tanques para el almacenamiento de combustibles que forman un complejo de depósitos con tuberías acopladas para la entrada y retirada de combustibles y eliminación de efluentes. Objetivo: Evaluar el uso de la bentonita en la remoción de cationes tóxicos de los vertidos generados en la empresa Petromoc. Materiales y Métodos: La recogida de muestras de bentonita se realizó en la empresa de Minerales Industriales. Las muestras efluente y afluente se colectaron en la caja separadora de la base Operacional. Los experimentos fisicoquímicos se ejecutaron en el Laboratorio de Química Analítica de la Universidad Eduardo Mondlane. Para la caracterización física de la bentonita, se determinaron la densidad picnométrica, porosidad (P) y superficie específica. La caracterización mineralógica se realizó por análisis de difracción de rayos X. La caracterización química del efluente y afluente se realizó mediante la Espectroscopía de Emisión Atómica por Plasma Inductivamente acoplado. Resultados y Discusión: La caracterización mineralógica demostró la presencia de minerales de bentonita. El pH del efluente antes de interactuar con la bentonita era de 3,22, tras la interacción el pH está entre 5,8 a 9. Se determinaron altas concentraciones de Cadmio (Cd), Cromo (Cr), Cobre (Cu), Titanio (Ti) y Zinc (Zn), en el efluente antes de la interacción con la bentonita. Después de la interacción, se verifica remoción de elementos tóxicos. Conclusiones: La bentonita demostró alta eficiencia en la remoción de cationes tóxicos.

RESUMEN In recent decades, glacier retreat has been observed in all the world’s mountain ranges. Over the last 20 years, glaciers in the Andes have lost mass at one of the highest rates on record. Particularly, glaciers in the northern Patagonian Andes show the highest rate of loss of all the Andean regions for the last decade. Detailed records of long-term variations in glacier extent are crucial to put current climate change into context and quantify its impact on mountain areas. In the present study, a reconstruction of the extension (length and area) of the Alerce glacier (41.15° S-71.81° W) for the last 70 years was carried out. By means of historical documents and satellite images (Landsat and Pléiades), detailed maps of glacier fluctuations were generated for the period 1953-2020. Four methodologies were evaluated to measure the front position: i) central flow line; ii) curvilinear box; iii) multiline; and iv) variable box. The magnitude of the front position variations depends on the methodology applied. The method that best addresses glacier front variations over the period analyzed is the variable box method. Between 1953 and 2020, the Alerce glacier lost 35% of its area (1.1±0.1 km2) and 67% of its total length (1.49±0.04 km). It is possible to distinguish periods with different rates of retreat. A stage of slight retreat (-11.3±0.5 ma-1) between 1953 and 1976, followed by a period of stability (-6.7±0.5 ma-1) between 1977 and 1983, then a strong retreat (-58.7±0.5 ma-1) between 1984 and 1999, followed by another period of stability (-5.4±0.5 ma-1) between 2000 and 2009 and, finally, another retreat (-8.7±0.5 ma-1) between 2010 and 2020. The retreat rates for the Alerce glacier mimic those observed in other glaciers in the region, possibly representing a regional climatic signal.

ABSTRACT En las últimas décadas se ha observado un retroceso de los glaciares en todos los cordones montañosos del mundo. Durante los últimos 20 años, los glaciares de los Andes perdieron masa a una tasa sin precedentes en el registro histórico. En particular, los glaciares de Patagonia norte son los que presentan la mayor tasa de pérdida de todas las regiones andinas para la última década. Contar con registros detallados sobre las variaciones en la extensión de los glaciares en el largo plazo es crucial para poner en contexto el cambio climático actual y cuantificar su impacto en zonas de montaña. En el presente estudio se realizó la reconstrucción de la extensión (área y longitud) del glaciar Alerce (41,15° S-71,81° O) para los últimos 70 años. Utilizando una combinación de documentos históricos e imágenes satelitales (Landsat y Pléiades), se generó cartografía de detalle para cuantificar las fluctuaciones del frente del glaciar Alerce para el período 1953-2020. Se evaluaron cuatro metodologías para medir la posición del frente: i) línea central de flujo; ii) caja curvilínea; iii) multilínea; y iv) caja variable. La magnitud de la variación del frente depende de la metodología aplicada. El método que mejor contempla la variación del frente del glaciar a lo largo del período analizado es el de caja variable. Entre 1953 y 2020, el glaciar Alerce perdió el 35% de su superficie (1,1±0,1 km2) y el 67% de su longitud total (1,49±0,04 km). Es posible distinguir períodos con diferentes tasas de retroceso. Una leve (-11,3±0,5 ma−1) entre 1953 y 1976, seguida de un período de relativa estabilidad (-6,7±0,5 ma−1) entre 1977 y 1983, luego un marcado retroceso (-58,7±0,5 ma−1) entre 1984 y 1999, otro período de estabilidad (-5,4±0,5 ma−1) entre 2000 y 2009 y, finalmente, otra etapa de retroceso (-8,7±0,5 ma−1) entre 2010 y 2020. Las tasas de retroceso para el glaciar Alerce coinciden con las observadas en otros glaciares de la región, lo cual puede representar una señal climática regional.

Resumo A coruja-das-torres americana (Tyto furcata) vive em ambientes urbanos e se alimenta principalmente de pequenos roedores, sendo de grande importância no controle biológico de pragas. O objetivo deste trabalho foi descrever os hábitos alimentares, parentais e reprodutivos de um casal de corujas-das-torres americanas, naturalmente vivendo fora de uma residência na zona urbana do município de Campos dos Goytacazes, estado do Rio de Janeiro, Brasil. Uma caixa de madeira foi instalada em uma parede externa da casa, monitorada por uma câmera de vídeo. Uma planilha foi criada para manter o controle das observações registradas. A fêmea pôs quatro ovos, e após período de incubação de 30-32 dias todos os ovos eclodiram, mas apenas dois filhotes sobreviveram após o canibalismo entre os filhotes. Inicialmente, o macho fornecia a comida aos filhotes e a fêmea permanecia no ninho cuidando da ninhada. Depois de cerca de um mês, a fêmea também começou a sair do ninho e voltar com a presa, que era oferecida aos filhotes, com o macho também continuando com esse comportamento. Os filhotes deixaram o ninho em setembro de 2017. Os dados obtidos mostram a existência de cooperação e divisão de tarefas entre corujas machos e fêmeas durante o período reprodutivo.

Abstract The American Barn Owl (Tyto furcata) lives in urban, periurban and wild environments and feeds mainly on small rodents, meaning it has great importance in the biological control of pests. The aim of this work was to describe the reproductive, parental and eating habits of a pair of American barn owls naturally living outside a residence in the urban area of the municipality of Campos dos Goytacazes, Rio de Janeiro state, Brazil. A wood box was installed on an outside wall of the home, monitored by a video camera. A spreadsheet was created to keep track of the observations recorded. The female laid four eggs, and after an incubation period of 30-32 days all the eggs hatched, but only two chicks survived after cannibalism among the chicks. Initially, the male provided the food to the chicks and the female remained in the nest caring for the brood. After approximately a month, the female also began to leave the nest and return with prey, which was offered to the chicks, with the male also continuing this behavior. The chicks left the nest in September, 2017. The data obtained show the existence of cooperation and division of tasks between male and female owls during the reproductive period.

Resumo O aumento da ansiedade e dos sintomas depressivos têm relatado sua associação com doenças de longa duração. Por causa dos efeitos indesejáveis dos novos medicamentos disponíveis, os pacientes que administram medicamentos ansiolíticos geralmente interrompem o tratamento antes de estarem completamente recuperados. Portanto, há uma necessidade séria de desenvolver novos medicamentos ansiolíticos. Foi avaliado o efeito ansiolítico do extrato hidroalcoólico de Agaricus blazei em modelos animais. Vinte e quatro camundongos machos (gênero Mus musculus) foram incluídos no estudo. Quatro grupos foram preparados, e cada grupo continha seis animais. Os grupos foram controle de veículo, controle positivo (diazepam 1,0 mg/kg, i.p.), bem como dois grupos de tratamento recebendo extrato hidroalcoólico de Agaricus blazei na dose de 136,50 mg/kg e 273,0 mg/kg por via oral. O teste de enterrar Marble, o teste de retalhamento Nestlet e o teste de caixa clara e escura são usados para avaliar a atividade ansiolítica. Camundongos foram administrados com diazepam 1,0 mg/kg, i.p., enquanto o extrato hidroalcoólico de AbM (136,50 e 273,0 mg/kg, respectivamente) foi administrado por via oral, que exibiu redução acentuada no número de mármores enterrados em comparação com o grupo de controle de veículo. Camundongos administrados com diazepam 1,0 mg/kg, i.p. e a administração oral de extrato hidroalcoólico de AbM (136,50 e 273,0 mg/kg, respectivamente) exibiu diminuição significativa na trituração de ninhos em comparação ao grupo de controle de veículo. A administração oral de extrato hidroalcoólico na dose de 136,5mg/kg e 273mg/kg mostrou elevação no tempo gasto na caixa de luz e foi comparável ao grupo tratado padrão, enquanto o tempo gasto por camundongos após a administração oral de extrato hidroalcoólico de Agaricus blazei na dose de 273,0 mg/kg também mostrou elevação e foi mais próximo do grupo tratado padrão (diazepam 1 mg/kg, ip).

Abstract Increased anxiety and depressive symptoms have reported to be its association with long term illness. Because of having unwanted effects of newly available drugs, patients administering anxiolytic drugs usually discontinue the treatment before they are completely recovered. Therefore, there is a serious need to develop new anxiolytic drugs. The anxiolytic effect of hydro-alcoholic extract of Agaricus blazei in animal models was assessed. 24 male mice (Mus musculus genus) were included in the study. Four groups were prepared and each group contained six animals. The groups were vehicle control, positive control (diazepam 1.0 mg/kg, i.p.) as well as two treatment groups receiving Agaricus blazei hydro-alcoholic extract at a dose of 136.50 mg/kg and 273.0 mg/kg orally. The Marble burying test, Nestlet shredding test and Light and Dark box test used to assess anxiolytic activity. Mice administered with diazepam 1.0 mg/kg, i.p. while hydro-alcoholic extract of AbM (136.50 and 273.0 mg/kg, respectively) was administered via oral route which exhibited marked reduction in number of marbles-burying as compared to vehicle control group. Mice administered with diazepam 1.0 mg/kg, i.p. and Oral administration of hydro-alcoholic extract of AbM (136.50 and 273.0 mg/kg, respectively) exhibited significant decrease in nestlet shredding in comparison to vehicle control group. The oral administration of hydro-alcoholic extract at a dose of 136.5mg/kg and 273mg/kg showed elevation in time spent in light box and was comparable to standard treated group while time spent by mice following oral administration of hydro-alcoholic extract of Agaricus blazei at a dose of 273.0 mg/kg also showed elevation and was found to be more near to standard treated group (diazepam 1 mg/kg, i.p.).

Resumo Em condições de estresse salino, o crescimento das plantas é reduzido, em virtude, do desequilíbrio osmótico, nutricional e oxidativo. Contudo, o ácido salicílico atua na mitigação desse estresse abiótico por promover incremento no crescimento, fotossíntese, metabolismo do nitrogênio, síntese de osmorreguladores e enzimas antioxidantes. Nesse contexto, objetivou-se avaliar o efeito de doses de ácido salicílico sobre o crescimento e alterações fisiológicas de mudas de berinjela sob estresse salino. O experimento foi conduzido em casa de vegetação, onde os tratamentos foram distribuídos em blocos ao acaso utilizando uma matriz composta central Box com cinco níveis de condutividade elétrica da água de irrigação (CEa) (0,50; 1,08; 2,50; 3,92 e 4,50 dS m-1), associada a cinco doses de ácido salicílico (AS) (0,00; 0,22; 0,75; 1,28 e 1,50 mM), com quatro repetições e cada parcela composta por três plantas. Aos 40 dias após a semeadura, foram determinados a altura da planta, diâmetro do caule, número de folhas, área foliar, vazamento de eletrólito, teor relativo de água e massa seca total. A CEa e a aplicação de AS influenciaram no crescimento e nas alterações fisiológicas das mudas de berinjela. O aumento da CEa reduziu o crescimento na ausência de AS. O dano de membrana com o uso de AS manteve-se estável até 3,9 dS m-1 de CEa. O conteúdo relativo de água independentemente da CEa aumentou com 1 mM de SA. O uso de AS na concentração de 1 mM mitigou o efeito deletério da salinidade no crescimento das mudas até 2,50 dS m-1 de CEa.

Abstract Under salt stress conditions, plant growth is reduced due to osmotic, nutritional and oxidative imbalance. However, salicylic acid acts in the mitigation of this abiotic stress by promoting an increase in growth, photosynthesis, nitrogen metabolism, synthesis of osmoregulators and antioxidant enzymes. In this context, the objective was to evaluate the effect of salicylic acid doses on the growth and physiological changes of eggplant seedlings under salt stress. The experiment was conducted in a greenhouse, where the treatments were distributed in randomized blocks using a central composite matrix Box with five levels of electrical conductivity of irrigation water (CEw) (0.50; 1.08; 2.50; 3.92 and 4.50 dS m-1), associated with five doses of salicylic acid (SA) (0.00; 0.22; 0.75; 1.28 and 1.50 mM), with four repetitions and each plot composed of three plants. At 40 days after sowing, plant height, stem diameter, number of leaves, leaf area, electrolyte leakage, relative water content, and total dry mass were determined. ECw and SA application influenced the growth and physiological changes of eggplant seedlings. Increasing the ECw reduced growth in the absence of SA. Membrane damage with the use of SA remained stable up to 3.9 dS m-1 of ECw. The relative water content independent of the CEw increased with 1.0 mM of SA. The use of SA at the concentration of 1.0 mM mitigated the deleterious effect of salinity on seedling growth up to 2.50 dS m-1 of ECw.

Resumo As comunidades quilombolas, estão presentes em diversos estados brasileiros, vivendo em condições de saúde mais precárias. Isto ocorre por conta do isolamento geográfico, das limitações de acesso e da falta de qualidade no serviço quando este é prestado. Nesse sentido, o objetivo do estudo foi analisar a qualidade de vida de mulheres de uma comunidade quilombola do nordeste brasileiro. Estudo observacional, transversal e descritivo. Foram entrevistadas 160 mulheres adultas, através de um formulário para a coleta de perfil e do questionário de Qualidade de Vida WHOQOL – bref. Foi observado que as mulheres tinham em média 40,7 anos (±17,25), casadas, autodeclaradas negras, com fundamental incompleto, do lar, sem renda, com moradia de alvenaria, própria, com até 6 cômodos, abastecidas por caixa de água comunitária, tratada. A Qualidade de Vida, apresentou escores medianos nos domínios: físico (3,18), psicológico (3,4), relações sociais (3,45) e meio ambiente (2,59). Com a realização desta pesquisa foi possível caracterizar a comunidade quilombola de Santa Luzia do Norte-AL quanto as dificuldades de acesso a saúde e geração de renda, fatos que repercutem na sua condição de saúde. Os problemas descritos neste estudo podem contribuir para que ações de saúde sejam planejadas e efetivadas com o intuito de melhorar as condições socioeconômicas e de saúde nessa comunidade, considerando-se o contexto social, político e ambiental, valorizando seus saberes e práticas tradicionais.

Abstract Quilombola communities are present in many Brazilian states living in precarious health conditions. This is due to geographic isolation, limitations to the access of the area in which they live in, and the lack of quality in the service when it is needed to be provided. Therefore, the aim of this study was to analyze the quality of life of women from a quilombola community in northeastern Brazil. It is an observational, cross-sectional and descriptive study. 160 adult women were first interviewed through a form to collect a profile and then it was applied the WHOQOL Quality of Life questionnaire – bref. It was observed that the women were on average 40.7 years old (±17.25), married, self-declared black, who did not finish elementary school, housewife, had no income, with their own masonry house, with up to 6 rooms, supplied by a box of community treated water. Quality of Life had median scores in the domains: physical (3.18), psychological (3.4), social relationships (3.45) and environment (2.59). With this research, it was possible to characterize the quilombola community of Santa Luzia do Norte-AL regarding the difficulties of access to health and income generation, issues that affect their health condition. The problems described in this study can contribute to health actions being planned and carried out in order to improve socioeconomic and health conditions in this community, considering the social, political and environmental context, valuing their traditional knowledge and practices.

Resumen El artículo analiza las estrategias dirigidas a profesores y alumnos del periódico Resumo Escolar, publicado en 1929; comprendiéndolo como un dispositivo pedagógico de difusión e implementación de principios de la reforma escolanovista. Basado en el método indiciário (Ginzburg, 2002), utiliza como fuentes: siete ediciones de la publicación investigada, informes gubernamentales e informes de inspección escolar. En el contexto de la reforma educativa elaborada en Espírito Santo entre 1928 y 1930, el proyecto editorial del periódico Resumo Escolar y las estrategias adoptadas por parte de la Secretaría de Instrucción Pública, se configuraron como una caja de utensilios (Carvalho, 2006) que transmitía el ideal de hombre nuevo y las orientaciones didáctico-pedagógicas que guiarían a las escuelas locales. 1929 escolanovista Ginzburg, Ginzburg (Ginzburg 2002, 2002 , 2002) fuentes investigada escolar 192 1930 Pública Carvalho, Carvalho (Carvalho 2006 didácticopedagógicas didáctico pedagógicas locales 200 19 193 20 1 2

Abstract The article analyzes the strategic uses of Resumo Escolar, published in 1929, in addressing teachers and students, understanding it as a pedagogical device for the dissemination and implementation of the principles of school reform in Espírito Santo. Based on the evidentiary method (Ginzburg, 2002), it uses as sources: seven editions of the investigated journal, government reports, school inspection reports and journalistic materials. In the context of the educational reform produced in Espírito Santo between 1928 and 1930, it is understood that the editorial project of Resumo Escolar and its strategic uses by the Secretariat of Public Education, conform it as a box of utensils (Carvalho, 2006) that conveyed the new man ideal and the didactic-pedagogical guidelines that would guide local schools. 1929 students Ginzburg, Ginzburg (Ginzburg 2002, 2002 , 2002) sources journal materials 192 1930 Education Carvalho, Carvalho (Carvalho 2006 didacticpedagogical didactic schools 200 19 193 20 1 2

Resumo O artigo analisa os usos estratégicos do Resumo Escolar, publicado em 1929, no endereçamento a professores/as e estudantes, compreendendo-o como dispositivo pedagógico para a divulgação e implementação dos princípios da reforma escolanovista no Espírito Santo. Com base no método indiciário (Ginzburg, 2002), utiliza como fontes sete edições do impresso investigado, relatórios de governo, relatórios de inspeção escolar e matérias jornalísticas. No contexto da reforma educacional produzida no Espírito Santo, entre 1928 e 1930, conclui que o projeto editorial do Resumo Escolar e os seus usos estratégicos pela Secretaria da Instrução Pública conformam-no como caixa de utensílios (Carvalho, 2006) que veiculava o ideal de homem novo e as diretrizes didático-pedagógicas que orientariam as escolas locais. 1929 professoresas professores estudantes compreendendoo compreendendo Santo Ginzburg, Ginzburg (Ginzburg 2002, 2002 , 2002) investigado governo jornalísticas 192 1930 conformamno conformam Carvalho, Carvalho (Carvalho 2006 didáticopedagógicas didático pedagógicas locais 200 19 193 20 1 2

ABSTRACT Weeds have several mechanisms of dispersal and reproduction, resulting high spatial variability. The objective of this study was to assess the scale and spatial heterogeneity of weeds using multifractal analysis in a no-tillage system in the Pre-Amazon region. Sampling was conducted in a commercial soybean (Glycine max) production plot in the Mata Roma, Maranhão, Brazil, comprising 1,071 points marked on a 10 × 10 m grid. Data were analyzed using descriptive statistics and multifractal analysis through the box-counting method. Weeds showed varying degrees of multifractality, resulting in different scales and spatial heterogeneity in the study area. Euphorbia hirta and Turnera subulata exhibited asymmetry of branches to the left in the singularity spectrum, indicating dominance of high measurement values. reproduction variability notillage no tillage PreAmazon Pre Amazon region Glycine max Roma Maranhão Brazil 1071 1 071 1,07 grid boxcounting box counting method multifractality area spectrum values 107 07 1,0 0 1,

RESUMO As plantas-daninhas possuem diversos mecanismos de dispersão e reprodução descrevendo elevada variabilidade espacial. O objetivo deste estudo foi avaliar a heterogeneidade de escala e espacial de plantas-daninhas utilizando análise multifractal em sistema de semeadura direta na região Pré-Amazônia. A amostragem foi realizada em uma parcela de produção comercial cultivada com soja (Glycine max). Na área de estudo foram demarcados 1.071 pontos, em malha regular de 10 × 10 m, no município de Mata Roma (Maranhão, Brasil). Os dados foram analisados por meio da estatística descritiva e análise multifractal empregando o método box-counting. As plantas-daninhas apresentaram diferentes graus de multifractalidade, descrevendo maior ou menor heterogeneidade de escala e espacial na área de estudo. Euphorbia hirta e Turnera subulata apresentaram assimetria dos ramos do espectro de singularidade para a esquerda, indicando domínio de valores elevados de medidas. plantasdaninhas plantas daninhas PréAmazônia. PréAmazônia Pré Amazônia. Amazônia Pré-Amazônia Glycine max. max . max) 1071 1 071 1.07 pontos m Maranhão, Maranhão (Maranhão Brasil. Brasil Brasil) boxcounting. boxcounting box counting. counting box-counting multifractalidade esquerda medidas 107 07 1.0 0 1.

ABSTRACT Introduction: Atrial fibrillation is one of the common complications of mitral valve disease. Currently, in the absence of freezing equipment, it’s still impossible to fully conduct a minimally invasive Cox-maze IV procedure to treat atrial fibrillation. Methods: We analyzed the clinical data of 28 patients who underwent thoracoscopic minimally invasive mitral valve full maze surgery in our hospital from October 2021 to September 2022; 13 patients were male and 15 were female, three suffered from paroxysmal atrial fibrillation, and 25 suffered from permanent atrial fibrillation; average age was 61.88±8.30 years, and mean preoperative left atrial diameter was 47.12±8.34 mm. Isolation of left atrial posterior wall (box lesion) was completed in all patients by cut-and-sew technique and bipolar clamp ablation. Results: For these subjects, the median cardiopulmonary bypass time was 169 (109.75-202.75) minutes, aortic cross-clamping time was 106 (77.75-125.50) minutes, and ventilator assistance time was 6.5 (0-10) hours. Among them, eight subjects had the endotracheal tubes removed immediately after surgical operation. Three subjects were in the blanking period; two subjects still had atrial fibrillation at three months after operation, one of whom resumed sinus rhythm after electrical cardioversion therapy; and all the remaining 23 subjects had sinus rhythm. Conclusion: The minimally invasive cut-and-sew technique for electrical isolation of left pulmonary veins can improve sinus conversion rate of patients suffering from both mitral valve disease and atrial fibrillation. In selected subjects, it is safe and has good results in the short-term postoperative period. Introduction Currently equipment its s Coxmaze Cox Methods 2 202 2022 1 female 6188830 61 88 8 30 61.88±8.3 years 4712834 47 12 34 47.12±8.3 mm box lesion cutandsew cut sew ablation Results 16 109.75202.75 1097520275 109.75 202.75 109 75 (109.75-202.75 minutes crossclamping cross clamping 10 77.75125.50 777512550 77.75 125.50 77 125 50 (77.75-125.50 65 6 5 6. 010 0 (0-10 hours them operation period therapy Conclusion shortterm short term 20 618883 3 61.88±8. 471283 4 47.12±8. 75202 109.75202.7 109752027 10975 109.7 20275 202.7 7 (109.75-202.7 75125 77.75125.5 77751255 7775 77.7 12550 125.5 (77.75-125.5 01 (0-1 61888 61.88±8 47128 47.12±8 7520 109.75202. 10975202 1097 109. 2027 202. (109.75-202. 7512 77.75125. 7775125 777 77. 1255 125. (77.75-125. (0- 6188 61.88± 4712 47.12± 752 109.75202 1097520 (109.75-202 751 77.75125 777512 (77.75-125 (0 618 61.88 471 47.12 109.7520 109752 (109.75-20 77.7512 77751 (77.75-12 ( 61.8 47.1 109.752 (109.75-2 77.751 (77.75-1 61. 47. (109.75- (77.75- (109.75 (77.75 (109.7 (77.7 (109. (77. (109 (77 (10 (7 (1