RESUMO: Vários resíduos agroindustriais podem ser utilizados para a extração de açúcares por meio de reações químicas. Este estudo avalia a extração de açúcares totais e açúcares redutores de resíduos de casca de banana (Musa paradisiaca) e laranja (Citrus sinensis) usando reações de hidrólise ácida para saber se há diferença entre a concentração de açúcares presentes, analisando o efeito de diferentes concentrações de ácido sulfúrico e tempos de reação. Foi utilizado o delineamento experimental 2 × 4 × 2 completamente aleatório, avaliando dois tipos de resíduos, expostos a quatro concentrações de ácido sulfúrico (4, 6, 8 e 10% V/V) em tempos de reação de 2 e 4 horas. Totalizando 16 tratamentos com três repetições. As unidades experimentais foram compostas por frascos de vidro de borosilicato semi-submersos em um banho de óleo vegetal e montados em uma torre de resfriamento rápido, de onde foram obtidos os xaropes de glicose analisados. Os resíduos de casca de laranja apresentaram os maiores valores de extração de açúcares redutores com concentração de 8% de ácido sulfúrico, apresentando média de 16,46 mg L-1 ± SE = 0,591 e tempo ótimo de reação de hidrólise de 2 horas. Com 4 horas de tempo de reação, a extração de açúcares redutores foi maior nos resíduos de casca de laranja em comparação com os resíduos de casca de banana. Ambos os resíduos se mostraram adequados para a extração de açúcar, apresentando uma valiosa oportunidade de utilização em regiões onde estão prontamente disponíveis. Eles podem ser empregados em processos de fermentação para a produção de bioetanol.

ABSTRACT Several agro-industrial residues can be utilized for the extraction of sugars through chemical reactions. This study evaluated the extraction of total sugars and reducing sugars from banana (Musa paradisiaca) and orange (Citrus sinensis) peel residues using acid hydrolysis. It also investigated if there is a difference between the concentration of sugars present, and analyzed the effect of different sulfuric acid concentrations and reaction times. A completely randomized 2 × 4 × 2 experimental design was used to evaluate two types of waste, exposed to four concentrations of sulfuric acid (4, 6, 8 and 10% V/V) at reaction times of 2 and 4 hours. This amounted to 16 treatments with three repetitions. The experimental units consisted of borosilicate glass flasks, half submerged in a bath of vegetable oil and assembled in a rapid cooling tower from which the analyzed glucose syrups were obtained. The orange peel residues showed the highest values of reducing sugar extraction with 8% sulfuric acid concentration, presenting mean of 16.46 mg L-1 ± SE = 0.591 and optimal hydrolysis reaction time of 2 hours. With 4 hours reaction time, the extraction of reducing sugars was higher in orange peel residues compared to banana peel residues. Both residues proved to be suitable for sugar extraction, presenting a valuable opportunity for utilization in regions where they are readily available. They can be employed in fermentation processes for bioethanol production.

RESUMO O objetivo desta pesquisa foi avaliar a influência de diferentes épocas de plantio e variáveis meteorológicas no desempenho produtivo da mandioca industrial na região do Agreste de Alagoas. Os tratamentos foram cinco datas de plantio, com espaçamento temporal de 25 dias entre elas: D1 - 10 de abril, D2 - 05 de maio, D3 - 30 de maio, D4 - 24 de junho e D5 - 19 de julho. O período experimental foi de abril de 2021 a setembro de 2023, dois ciclos de cultivo. Utilizou-se o delineamento em blocos casualizados, com quatro repetições. As variáveis analisadas foram: dados agrometeorológicas e de crescimento e produção da cultura. Na região do Agreste de Alagoas, entre as estações chuvosa e seca, a ET0 média diária varia de 2,9 a 4,7 mm, respectivamente. A temperatura média do ar é 24,3 °C e atende as demandas térmicas da cultura da mandioca. A precipitação anual é suficiente para garantir a produção de mandioca, porém, há sazonalidade nas chuvas, com período seco da primavera ao verão, o que prejudica plantios mais tardios. Na região do Agreste de Alagoas, o melhor período para o plantio da mandioca é de 10 de abril a 5 de maio, que é o início do período chuvoso da região e garante melhores resultados em: altura do dossel vegetativo (3,2 m), diâmetro do caule (2,8 cm), índice de área foliar (3,0), comprimento máximo (43,1 cm), número (6,6) e rendimento de raízes (72,8 t ha-1), biomassa total (145 t ha-1) e teor de amido (34,3%).

ABSTRACT The aim of this study was to evaluate the influence of different planting times and meteorological variables on the productive performance of industrial cassava in the Agreste region of Alagoas state, Brazil. The treatments were five planting dates, 25 days apart: D1 - April 10, D2 - May 5, D3 - May 30, D4 - June 24 and D5 - July 19. The experiment was conducted from April 2021 to September 2023 (two cultivation cycles), using randomized blocks, with four replicates. The variables analyzed were agrometeorological data, crop growth and yield. In the Agreste region of Alagoas, the average daily ET0 varies from 2.9 to 4.7 mm between the rainy and dry seasons, respectively. The average temperature of 24.3 °C meets the thermal demands of cassava cultivation. Although annual rainfall is sufficient to guarantee cassava production, it is seasonal, with a dry period from spring to summer, which compromises subsequent plantings. In the Agreste region of Alagoas, the best time to plant cassava is from April 10 to May 5, which is the beginning of the rainy season and ensures better vegetative canopy height (3.2 m), stem diameter (2.8 cm), leaf area index (3.0), maximum root length (43.1 cm), number (6.6) and yield (72.8 t ha-1), total biomass (145 t ha-1) and starch content (34.3%).

Abstract Proper nutritional management is crucial for the development and production of cannabinoids in the cultivation of Cannabis sativa var. Cherry Oregon. The objective of this research was to evaluate the effect of five concentrations of Steiner nutrient solution on the phenology of medicinal hemp, and to determine the optimal dosage of the nutrient solution for cannabinoid content (cannabidiol [CBD] and tetrahydrocannabinol [THC]). Research was conducted at the Instituto Nacional de Investigaciones Agropecuarias [INIAP], Estación Experimental Santa Catalina [EESC], in Ecuador, under greenhouse conditions. Five concentrations of Steiner nutrient solution (50, 75, 100, 125 and 150%). Agronomic variables such as plant height, diameter at collar height, yield, and cannabinoid content in flowers were evaluated. Significant effects (p > 0,05) were used. Agronomic variables such as plant height, diameter at neck height, yield, and cannabinoid content in flowers were evaluated. Significant effects (p > 0.05) were observed in plant height and diameter at neck height. Tetrahydrocannabinol (THC) remained at concentrations lower than 1%. Concentrations at 75% and 100% of the nutrient solution showed higher levels of cannabidiol (CBD) with 15,3% and 15,15%, respectively. The nutrient solution at 125% and 100% concentrations promoted greater growth in plant height and diameter. The 125% concentration also produced the highest dry biomass yield with 810 g m-2 or 8,1 t ha-1, surpassing the other concentrations. It is concluded that the optimal concentration of Steiner solution was 125% for accumulation of dry biomass, and 75 and 100% for cannabinoid production in flowers (CBD), complying with current regulations (< 1% THC).

Resumen El adecuado manejo nutricional es crucial para el desarrollo y producción de cannabinoides en el cultivo de Cannabis sativa var. Cherry Oregon. Esta investigación tuvo por objetivo evaluar el efecto de cinco concentraciones de solución nutritiva Steiner en la fenología del cáñamo medicinal, y determinar la dosis óptima de la solución nutritiva para el contenido de cannabinoides (cannabidiol [CBD] y tetrahidrocannabinol [THC]). La investigación fue realizada en el Instituto Nacional de Investigaciones Agropecuarias [INIAP], Estación Experimental Santa Catalina [EESC], en Ecuador, en condiciones de invernadero. Se emplearon cinco concentraciones de solución nutritiva de Steiner (50, 75, 100, 125 y 150%). Se evaluaron variables agronómicas como altura de planta, diámetro a la altura del cuello, rendimiento y contenido de cannabinoides en las flores. Se observó efecto significativo (p > 0,05) en altura de planta y diámetro del cuello. El THC se mantuvo < 1%. Concentraciones al 75% y 100% de la solución nutritiva mostraron niveles superiores de CBD con 15,3 y 15,15%, respectivamente. La solución nutritiva al 125 y 100% promovieron un mayor crecimiento en altura y diámetro a la altura del cuello. La concentración al 125% también produjo mayor rendimiento de biomasa seca con 810 g m-2 o 8,10 t ha-1, superando al resto de concentraciones. Se concluye que la concentración óptima de solución Steiner fue de 125% para acumulación de biomasa seca, y del 75 y 100% para la producción de cannabinoides en flores (CBD), cumpliendo con las regulaciones vigentes (< 1% de THC).

Abstract (Introduction): In the removal of chromium (VI) from tannery wastewater, physicochemical methods are the most used. However, these require high operating costs, and worse still, generate secondary pollutants that again require complementary treatments for their elimination. Given this, the applicability of vegetable organic biomass (VOB) emerges as an environmental alternative based on: the simplicity of its obtaining, the reduction as waste, it does not generate secondary pollutants and, above all, it removes chromium (VI). (Objective): Use VOB waste in an aerobic sequential biological reactor (SBR) for the removal of chromium (VI) in the treatment of tannery wastewater. (Methodology): 3 aerobic SBR systems were implemented; 2 controls (SBR1 and SBR2) and 1 study subject (SBR+VOB). Each system was fed with tannery wastewater (18 L) and connected to an aeration mechanism (12.7 L/min). The bacterial inoculum, a fundamental part of an SBR system, was gained from the selective culture of chromium (VI) reducing bacteria (RBCrVI) taken from the wastewater under study, and integrated into SBR2 and SBR+VOB at 0.6 g/L. The VOB was obtained from Stenotaphrum secundatum residues, fragmented, sieved, sterilized and integrated into the SBR+VOB at 1.5 g/L. (Results): The SBR+VOB system achieved the highest percentage of chromium (VI) removal (93 %), followed by SBR2 (80.1 %), and finally SBR1 (2.8 %). With respect to COD and TSS, the statistical test (Tukey) showed that there was no significant difference between the system under study and the controls, recording COD removal percentages ranging from 87.8 % to 88.4 %, and TSS ranging from 63.8 % to 69.2 %. (Conclusions): The viability of using VOB waste to remove chromium (VI) in the treatment of tannery wastewater has been evidenced. The results of this research create a promising panorama to mitigate the effects derived from inadequate management of tannery wastewater and organic solid waste.

Resumen (Introducción): En la remoción de cromo (VI) de aguas residuales de curtiembre, los métodos fisicoquímicos son los más empleados. Sin embargo, estos demandan altos costos operativos y, peor aún, generan contaminantes secundarios que nuevamente requieren tratamientos complementarios para su eliminación. Ante ello, la aplicabilidad de la biomasa orgánica vegetal (BOV) emerge como una alternativa ambiental sobre la base de: la simplicidad de su obtención, la disminución como residuo, no genera contaminantes secundarios y, sobre todo, de remover cromo (VI). (Objetivo): Usar residuos de BOV en un reactor biológico secuencial (RBS) aerobio, para la remoción de cromo (VI) en el tratamiento de agua residual de curtiembre. (Metodología): Se implementaron 3 sistemas RBS aerobios; 2 controles (RBS1 y RBS2) y 1 materia de estudio (RBS+BOV). Cada sistema fue alimentado con agua residual de curtiembre (18 L) y conectado a un mecanismo de aireación (12.7 L/ min). El inóculo bacteriano, parte fundamental de un sistema RBS, se obtuvo a partir del cultivo selectivo de bacterias reductoras de cromo (VI) (BRCrVI), tomadas del agua residual en estudio e integradas al RBS2 y RBS+BOV a 0.6 g/L. La BOV se obtuvo de residuos de Stenotaphrum secundatum, fragmentado, tamizado, esterilizado e integrado al RBS+BOV a 1.5 g/L. (Resultados): El sistema SRB+BOV alcanzó el mayor porcentaje de remoción de cromo (VI) (93 %), seguido por SBR2 (80.1 %) y, por último, de RBS1 (2.8 %). Con respecto a la DQO y SST, la prueba estadística (Tukey) arrojó que no había diferencia significativa entre el sistema en estudio y los controles, por lo que registró porcentajes de remoción de DQO que van de 87.8 % a 88.4 % y SST que van de 63.8 % a 69.2 %. (Conclusiones): Se ha evidenciado la viabilidad del aprovechamiento de residuos de BOV para remover cromo (VI) en el tratamiento de agua residual de curtiembre. Los resultados de la presente investigación crean un panorama prometedor para mitigar los efectos derivados de la inadecuada gestión de las aguas residuales de curtiembre y de los residuos sólidos orgánicos.

ABSTRACT Medicinal plants (MP) are used in various forms (teas, tinctures, creams, food supplements, etc.), with a single purpose, that of improving the quality of human life from birth to senescence. The choice of the species Cassia angustifolia Vahl, to be introduced into culture and for the development of mechanized cultivation technology is argued by the high demand for ecological raw material, correlated with climate changes. Establishing the optimal planting distance is very important in crop management to improve plant growth and yield. In this study, the effects of planting density on the growth and yield of C. angustifolia plants were evaluated in the edaphoclimatic conditions of Brasov, Romania. The experiment included three factors: Factor A - experimental year: 5 April 2016 (A1), 11 April 2017 (A2), 4 April 2018 (A3). Factor B - Distance row by row (RR): 25 (B1), 50 (B2), and 70 cm (B3). Factor C - Distance plant by plant (PP): Continuous row (C1), 15 cm (C2), and 25 cm (C3). Three years’ data indicated that plant density of 25 (RR) × continuous row (PP) resulted in obtaining the highest yield of fresh (48.33 t ha-1) and dry (7.93 t ha-1) biomass. Increasing planting density increased individual plant development (height, number of branches and weight), but reduced yield of biomass.

ABSTRACT Intercropping has been widely studied owing to its potential to increase crop production and improve soil properties. The combination of wheat (Triticum aestivum L.) with chickpea (Cicer arietinum L.) and lupin (Lupinus albus L.), along with P fertilization, has shown promising results in enhancing wheat yield. This study investigated the effects of intercropping wheat with chickpea and lupin, as well as P fertilization, on wheat yield, soil properties, and root morphological characteristics. A field experiment was conducted to evaluate chemical properties, basal soil respiration, and enzymatic activity. The results revealed that intercropping with chickpea and lupin, along with the addition of P, significantly increased the relative wheat yield compared with monoculture. The land equivalent ratio (LER) was also influenced by P fertilization, with wheat/lupin showing higher values than wheat/chickpea. Furthermore, aerial biomass exhibited significant differences between cropping systems and P fertilization, with the P+ treatment (220 kg ha-1) resulting in 24% higher aerial biomass during anthesis in the wheat/chickpea intercropping system than in wheat monocrops. The findings of this study indicate that the cropping system did not have a substantial impact on soil chemical properties. However, the interaction between cropping system and P fertilization had a significant effect on soil basal respiration. Although phosphatase activity remained unchanged by the cropping system and P fertilization, urease activity was significantly influenced by both factors, with a notable interaction effect.

ABSTRACT Quinoa (Chenopodium quinoa Willd.) exhibits remarkable adaptability to different agroecological conditions, but there are still challenges in introducing it to new environments, especially those in northern latitudes. This study investigates the impact of genotype, season, sowing density (5 and 10 cm between plants), and their interaction on agro-morphological traits of quinoa cultivars (Puno and Titicaca). Seasonal variations, primarily influenced by precipitation, significantly affected all analyzed traits (plant height, number of side branches and flower branches, biomass and grain yield per plant) with the highest values recorded in a favorable season. The season factor had the greatest influence in the variation of grain yield, with a share of 89.7% in the total variation. Grain yield per plant was 31.41 g in the favorable season compared to 14.64 g in the less favorable season. The significance of the Genotype × Season interaction in variation of plant height, number of side branches and biomass production per plant, as well as the significance of the Season × Sowing density interaction in biomass production, highlighted the importance of these factors to optimize cultivation practices and increase quinoa productivity in different environmental conditions. Principal component analysis (PCA) highlighted the interrelationships among agro-morphological traits, where in less favorable environments grain yield was most closely related to biomass, number of side branches and number of flower branches. Lower sowing density (10 cm between plants) may be more favorable for achieving high values across all analyzed traits, particularly increasing the number of flower branches (16.75 at lower density and 14.61 at higher density). The ‘Puno’ genotype stands out with a pronounced adaptability to less favorable environmental conditions, especially at lower sowing density. In contrast, the ‘Titicaca’ genotype stands out for its high biomass production, especially in the second season (74.9 g).

RESUMO A disposição de resíduos sólidos em aterros sanitários é um grande desafio para o setor de celulose e papel, e sua utilização como fertilizantes e corretivos de solo tem apresentado potencial como uma nova alternativa de destino. Assim, este trabalho objetivou identificar os fatores que influenciam esta alternativa de destinação dos resíduos e validar sua viabilidade técnica, legal e econômica através de uma revisão sistemática de literatura, de acordo com os principais itens para relatar revisões sistemáticas e meta-análises. De forma geral, foram identificados como benefícios o aumento da fertilidade e controle da acidez do solo através da aplicação de lodo de estações de tratamento de efluentes, resíduos da recuperação do licor verde e cinzas de biomassa, e os teores de metais tóxicos se mantiveram abaixo dos limites da legislação. Estes resultados, além de alavancarem ambientalmente o setor de celulose e papel pela destinação adequada de resíduos industriais, promovem a circularidade no manejo florestal e culturas agrícolas. Em relação aos aspectos econômicos, no entanto, não foram encontrados trabalhos conclusivos, sendo necessário o desenvolvimento de um estudo robusto para comprovação da viabilidade econômica desta aplicação.

ABSTRACT Industrial waste disposal in landfills is a challenge for the pulp and paper industry, and its use as fertilizers and soil conditioners has exhibited potential as a new alternative destination. As such, the present study aimed to identify factors that affect this alternative use for these residues and validate its technical, legal and economic feasibility through a systematic literature review based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocol. In general, applying wastewater treatment plant sludge, green liquor dregs and biomass ashes in the field has been shown to increase soil fertility, control its acidity, and maintain toxic metal content within the recommended limits. This enables the environmental leveraging of the pulp and paper sector by providing an appropriate alternative destination for industrial waste and promotes circularity in forest and agricultural management. However, no conclusive studies on the economic aspects of this alternative application were found, demonstrating the importance of conducting comprehensive research to confirm its economic feasibility.

RESUMO: As características químicas da biomassa lignocelulósica determinam sua adequação como fonte de energia, afetando sua combustibilidade, estabilidade de chama e eficiência energética geral. Portanto, este estudo avaliou a correlação entre as características químicas e o poder calorífico superior (PCS) nos componentes da biomassa de eucalipto (madeira, casca, galhos e folhas) de três espécies (E. benthamii, E. dunnii e E. saligna). Os resultados revelaram composições químicas variadas entre os componentes, o que impactou o PCS de forma diferente. Notavelmente, os materiais voláteis influenciaram positivamente o PCS, enquanto o teor excessivo de cinzas afetou negativamente o potencial energético. Correlações positivas com o PCS foram observadas para o teor de extrativos totais na casca, teor de lignina total nas folhas, teor de materiais voláteis na casca, folhas e caule e teor de carbono fixo no caule. Por outro lado, o teor de holocelulose reduziu o PCS da casca. Os materiais voláteis, exceto nos galhos, aumentaram eficientemente o PCS. Nenhuma das características químicas teve impacto significativo no PCS dos galhos.

ABSTRACT: The chemical characteristics of lignocellulosic biomass determine its suitability as an energy source, affecting its combustibility, flame stability, and overall energy efficiency. Therefore, this study assessed the correlation between the chemical characteristics and higher heating value (HHV) in Eucalyptus biomass components (wood, bark, branches, and leaves) of three species (E. benthamii, E. dunnii, and E. saligna). The results revealed varying chemical compositions among the components and species, which impacted the HHV differently. Notably, volatile materials positively influenced the HHV, whereas excessive ash content negatively affected the energy potential. Positive correlations with HHV were observed for the total extractives content in the bark, total lignin content in the leaves, volatile materials content in the bark, leaves, and stem, and fixed carbon content in the stem. Conversely, the holocellulose content reduced the bark HHV. Volatile materials, except in the branches, efficiently increased the HHV. None of the chemical characteristics had a significant impact on the branches HHV.

ABSTRACT The search for cropping systems that aim to have the best area use, optimize production, and guarantee financial profitability to the producer and their establishment in the field are challenges of modern research. Thus, the objective of this study was to evaluate the productive and monetary viability of intercropping radish and coriander fertilized with equitable amounts of hairy woodrose and roostertree biomass from the Caatinga ecosystem at different coriander population densities in two cropping seasons. The experimental design used was randomized blocks, with treatments arranged in a 4 × 4 factorial scheme with four replications. The first factor was constituted by four equitable amounts of hairy woodrose and roostertree biomass (20, 35, 50 and 65 t ha-1 on a dry basis), and the second factor was constituted by coriander population densities (40, 60, 80 and 100% of the recommended density for monocropping, RDM). The greatest agronomic and monetary advantages of intercropping radish with coriander were obtained with a land equivalent coefficient and a monetary equivalence ratio of 0.42 and 0.76, respectively, in the amounts of hairy woodrose and roostertree biomass of 20 and 39 t ha-1 added to the soil, in the coriander population density of 100% of the RDM. The coriander green mass yield and commercial productivity of radish roots optimized in the intercropping were 1.25 and 14.90 t ha-1 when fertilized with 65 t ha-1 of green manures biomass in the coriander population density of 100% RDM.

ABSTRACT Breeding of new cultivars of sugarcane uses several species belonging to the Saccharum complex genus. In this study, a set of 20 genotypes from the Saccharum complex was assessed for their growth promotion during germination using a mixed inoculant containing five selected diazotrophs and their resistance to short heat treatment (52°C / 30 min). The percentage of germination was evaluated, leading to the identification of three response groups. Saccharum officinarum (group I) showed the highest germination percentage, succeeded by Erianthus arundinaceus genotypes (group II) and the other genotypes (group III). The mean germination time (MGT) varied among the genotypes and segregated the growth response into two groups. Saccharum officinarum only required less than 15 days to reach the MGT, whereas the other genera required more than two or three days during the initial growth process. Bacterial counting also differed between the groups, being effective for S. officinarum and other genotypes, improving the colonization of the plants 100–1,000 times higher than the control and heat treatments. Further comparison was made among eight genotypes, including one Saccharum spontaneum(US72-1319), one S. officinarum (IN845), and six E. arundinaceus. This comparison measured biomass accumulation and root parameters. The E. arundinaceus group demonstrated less susceptibility to the heat treatment, resulting in stimulation of the root dry mass of IJ76-381 and root parameters of this genotype, as well as IJ76-364, although not associated to the inoculation treatment. Inoculation applied to US72-1319 and IN845 enhanced only the Shoot/Root ratio.

Due to the expansion of farming, many agricultural systems now depend more on chemical pesticides and inorganic fertilisers to boost farm output. Leguminous cover crops are used to improve soil fertility and to increase nitrogen availability for crop production. This review focuses mainly on evaluating the role of leguminous cover crops on soil's physical, chemical and biological properties. Furthermore, it focuses on the role of cover crops in crop biomass, grass nutritive value and crop-livestock grazing systems. This review used the Web of Science, Scopus and Google Scholar databases (accessed between January 2003 and December 2022). To find publications in the scope of the study, the authors combined different groups of keywords. The reviewed literature revealed that leguminous cover crops significantly increase yield due to an increase of nitrogen through nitrogen fixation. In addition, leguminous cover crops boost the forage's nutritional value, lowering feed costs and increasing livestock productivity. As a result of these findings, farmers can reap the benefits of leguminous cover crops in various ways, including enhancement of soil health, biomass, yield and reducing overall production costs.

RESUMEN La torta residual de Jatropha curcas es un subproducto generado durante la extracción del aceite de Jatropha curcas. Crea un serio problema ambiental al acumularse por no poder ser utilizada como alimento animal ni tener otro destino de utilidad, por lo que su aprovechamiento permitiría dar solución a diferentes problemáticas ambientales originadas tanto por la generación y disposición de estos residuos como por otros factores producto del desarrollo de otros sectores productivos. El cultivo de Pleurotus ostreatus se puede hacer en diferentes sustratos lignocelulósicos siendo los residuos agrícolas y agroindustriales los más utilizados. El objetivo del presente trabajo es evaluar el crecimiento micelial de Pleurotus ostreatus CCEBI 3024, en cultivos sólidos a base de extractos de la torta residual de Jatropha curcas. El estudio se basó en cultivar el inóculo del Pleurotus ostreatus en medio sólido a base de extractos de torta residual de la Jatropha curcas tóxica, no tóxica y una mezcla de ambas en proporción 1:1 y se comparó con el crecimiento micelial en el medio convencional agar nutriente. La medición del crecimiento micelial del hongo y la descripción morfológica del micelio evidencian el crecimiento adecuado del micelio de Pleurotus ostreatus en los medios evaluados, permitiendo afirmar que el empleo de la torta residual de Jatropha curcas como medio de cultivo para potenciar el crecimiento micelial y la formación de biomasa de Pleurotus ostreatus es factible en la propuesta de emplear este subproducto como sustrato en la producción de setas Pleurotus por FES

ABSTRACT Jatropha curcas residual cake is a by-product generated during the extraction of Jatropha curcas oil. It creates a serious environmental problem by accumulating because it cannot be used as animal feed or have another useful destination, so its use would allow a solution to different environmental problems caused by both the generation and disposal of this waste and other factors of the product development of other productive sectors. The cultivation of Pleurotus ostreatus can be done in different lignocellulosic substrates, with agricultural and agroindustrial waste being the most used. The objective of this work is to evaluate the mycelial growth of Pleurotus ostreatus CCEBI 3024, in solid cultures based on extracts from the residual cake of Jatropha curcas. The study was based on cultivating the inoculum of Pleurotus ostreatus in solid medium based on residual cake extracts of toxic and non-toxic Jatropha curcas and a mixture of both in a 1:1 proportion and was compared with mycelial growth in conventional medium nutrient agar. The measurement of the mycelial growth of the fungus and the morphological description of the mycelium show the adequate growth of the mycelium of Pleurotus ostreatus in the evaluated media, allowing us to affirm that the use of the residual cake of Jatropha curcas as a culture medium to enhance mycelial growth and the formation of Pleurotus ostreatus biomass is feasible in the proposal to use this byproduct as a substrate in the production of Pleurotus mushrooms by FES.

ABSTRACT Plants require iron (Fe) and copper (Cu) at low concentrations for metabolic functions; however, excess soil Fe and Cu can cause toxicity and affect plant development. The objective of this study was to assess the effects of soil Cu and Fe concentrations on seedling emergence, as well as on the metabolism of the main reserve compounds and the biomass in roots, stems, leaves, and cotyledons of Canavalia ensiformis. A greenhouse experiment was conducted in a completely randomized design; the Cu and Fe treatments in the soil were: (control, 50, 150, 250 and 350 mg dm-3) over a period of 10 days. Seedling emergence was not affected by the evaluated treatments; however, the results showed an increase in the emergence speed index (EVI) for the Fe treatment at a dose of 150 mg dm-3 of soil compared to 350 mg dm-3 of soil. Treatment with Cu at a dose of 350 mg dm-3 of soil reduced root length. Cu treatments reduced root dry mass. Fe treatments affected the content of soluble amino acids in the stems, leaves and cotyledons, as well as the total soluble proteins in the cotyledons and the carbohydrate content in the leaves. Cu treatments increased protein and carbohydrate content in leaves and starch content in cotyledons. High concentrations of Cu and Fe modify the production of nitrogenous compounds in the plant and reduce root biomass. (Fe (Cu functions however development roots stems ensiformis design were control, control (control 50 25 35 dm3 dm 3 1 days EVI (EVI 15 dm- length mass 5 2

RESUMO As plantas necessitam de ferro (Fe) e cobre (Cu) em baixas concentrações para realizar funções metabólicas. Porém, o excesso de Fe e Cu no solo pode causar toxicidade e afetar o desenvolvimento das plantas. O objetivo desse estudo foi avaliar os efeitos das concentrações de Cu e Fe no solo na emergência das plântulas, bem como no metabolismo dos principais compostos de reserva e na biomassa de raízes, caules, folhas e cotilédones de Canavalia ensiformis. O experimento foi conduzido em casa de vegetação, em delineamento inteiramente casualizado; os tratamentos de Cu e Fe no solo foram: (controle, 50, 150, 250 e 350 mg dm-3) durante um período de 10 dias. A emergência das plântulas não foi afetada pelos tratamentos avaliados; entretanto, os resultados mostraram aumento do índice de velocidade de emergência (EVI) para o tratamento com Fe na dose de 150 mg dm-3 de solo em comparação com 350 mg dm-3 de solo. O tratamento com Cu na dose de 350 mg dm-3 de solo reduziu o comprimento das raízes. Os tratamentos com Cu reduziram a massa seca das raízes. Os tratamentos com Fe afetaram o conteúdo de aminoácidos solúveis nos caules, folhas e cotilédones, bem como as proteínas solúveis totais nos cotilédones e o conteúdo de carboidrato nas folhas. Os tratamentos com Cu aumentaram os teores de proteínas e carboidratos nas folhas e o teor de amido nos cotilédones. Altas concentrações de Cu e Fe modificam a produção de compostos nitrogenados na planta e diminuem a biomassa radicular. (Fe (Cu metabólicas Porém raízes caules ensiformis vegetação casualizado foram controle, controle (controle 50 25 35 dm3 dm 3 1 dias avaliados entretanto EVI (EVI 15 dm- radicular 5 2

In the Amazon, Euterpe oleracea Mart. (açaí), is part of the identity of traditional communities, and the fruit is integrated into their everyday sociocultural dynamics. Hydrolysis and pyrolysis of residual biomass can be alternatives to yield sugars, ethanol, biochar and bio-oil. The açaí seeds subjected to oxidative pretreatment followed by acid hydrolysis increased cellulose to fermentable sugars (reaching 23.5%) and second-generation ethanol production (17.441 L t−1). Açaí oil (ca. 30%) was extracted using a Soxhlet apparatus (using hexane as solvent) and detected through gas chromatography coupled to mass spectrometry (GC-MS), where acids accounted for 82.43% of the total composition, while alcohols comprised only 13.15%. For pyrolysis, temperatures (500 and 550 °C), time (20 and 30 min), and activation temperature (750 and 800 °C) were evaluated, resulting in yields of ca. 22% for biochar and 30% for bio-oil. For the bio-oil, the main compounds detected by GC-MS were acids (16.3%), aldehydes (13.08%), amides (12.19%), alcohols (6.72%), and phenols (4.64%). The obtained biochar displayed surface area values of 553.7 m2 g−1 and 10% crystallinity, indicating its more amorphous nature. Amazon Mart açaí, , (açaí) communities dynamics biooil. biooil bio oil. bio-oil reaching 23.5% 235 23 5 secondgeneration second generation 17.441 17441 17 441 (17.44 t−1. t1 t t−1 . 1 t−1) ca (ca solvent GCMS, GCMS GC MS (GC-MS) 8243 82 43 82.43 composition 1315 13 15 13.15% 500 (50 55 °C, C °C 20 (2 3 min, min min) 750 (75 80 evaluated 22 biooil, oil, 16.3%, 163 16.3% 16 (16.3%) 13.08%, 1308 13.08% 08 (13.08%) 12.19%, 1219 12.19% 12 19 (12.19%) 6.72%, 672 6.72% 6 72 (6.72%) 4.64%. 464 4.64% 4 64 (4.64%) 5537 553 7 553. m g1 g g− 10 crystallinity nature (açaí 23.5 2 17.44 1744 44 (17.4 t− (GC-MS 824 8 82.4 131 13.15 50 (5 ( 75 (7 16.3 (16.3% 130 13.08 0 (13.08% 121 12.19 (12.19% 67 6.72 (6.72% 46 4.64 (4.64% 23. 17.4 174 (17. 82. 13.1 16. (16.3 13.0 (13.08 12.1 (12.19 6.7 (6.72 4.6 (4.64 17. (17 13. (16. (13.0 12. (12.1 6. (6.7 4. (4.6 (1 (16 (13. (12. (6. (4. (13 (12 (6 (4