Abstract Introduction. “Green mold,” caused by Penicillium digitatum is one of the phytosanitary issues that limits citrus production. For its control, synthetic fungicides are used, which have lost effectiveness due to resistance developed by the fungus. An alternative is the use of endophytic fungi with biocontrol action. Objective. To evaluate the effect of secondary metabolites (SM) from endophytic fungi (EF) on the mycelial inhibition of Penicillium digitatum and the effectiveness of disease control in mandarin fruits (Citrus reticulata) post-harvest. Materials and methods. The study was conducted in 2022, using a completely randomized experimental design in the Plant Health Laboratory at the Universidad Nacional de Cañete (UNDC), Lima, Peru. For the antibiosis test, secondary metabolites were extracted from ten strains of EF from the genera Trichoderma sp., Fusarium sp., and Aspergillus sp., where mycelial inhibition of the pathogen (%) was evaluated. SMs were sprayed at 15 % (v/v) concentration on mandarin fruits that were disinfected and inoculated with the pathogen by spraying (1x 105 CFU/cc). Additionally, a commercial biocontrol, a vegetable oil, and a fungicide were used. After 6 and 9 days, disease incidence (%) and severity index (0, 1, 2, 3) were evaluated. Results. Secondary metabolites from strains the HEA-111 (Aspergillus), HEA-109 (Fusarium), and HSA-1 (Trichoderma) showed the highest mycelial inhibition with 95.05 %, 87.51 %, and 47.47 %, respectively. In mandarin fruits treated with SMs from strains HEA-109 and HSA-1, there was a control of 80 % and 55 %, respectively. Conclusions. SMs from EF were effective in inhibiting the mycelial growth of P. digitatum and reduces disease progression in mandarin fruits post-harvest.

Resumen Introducción. El “moho verde” causado por Penicillium digitatum es uno de los problemas fitosanitarios que limita la producción en los cítricos. Para su control se utilizan fungicidas sintéticos, que han perdido eficacia, debido a la resistencia generada por el hongo. Una alternativa, es el uso de hongos endófitos con acción biocontroladora. Objetivo. Evaluar el efecto de metabolitos secundarios (MS) de hongos endófitos (HE) en la inhibición micelial de Penicillium digitatum y la eficacia de control de la enfermedad, en frutos de mandarina (Citrus reticulata) después de la cosecha. Materiales y métodos. El estudio se realizó en 2022, bajo un diseño experimental completo al azar, en el laboratorio de Sanidad Vegetal, Universidad Nacional de Cañete (UNDC), Lima, Perú. Para la prueba de antibiosis se extrajeron los metabolitos secundarios de diez cepas de HE de los géneros Trichoderma sp., Fusarium sp. y Aspergillus sp., en donde se evaluó la inhibición micelial del patógeno (%). Se asperjaron MS a una concentración del 15 % (v/v) sobre frutos de mandarina que fueron desinfectados e inoculados con el patógeno por aspersión (1x105 UFC/cc). Adicionalmente, se utilizaron un biocontrol comercial, un aceite vegetal y un fungicida. Después de 6 y 9 días, se evaluaron la incidencia (%) y el índice de severidad (0,1,2,3) de la enfermedad. Resultados. Los metabolitos secundarios de las cepas HEA-111 (Aspergillus), HEA-109 (Fusarium) y HSA-1 (Trichoderma) presentaron la mayor inhibición micelial con 95,05 %, 87,51 % y 47,47 %, respectivamente. En los frutos de mandarina tratados con los MS de las cepas HEA-109 y HSA-1, hubo un control del 80 % y 55 %, respectivamente. Conclusiones. Los MS de HE tuvieron efecto en la inhibición micelial de P. digitatum y redujeron el avance de la enfermedad en frutos de mandarina después de la cosecha.

Abstract Introduction: Bioindicator monitoring is a crucial conservation strategy within natural protected areas. Various taxonomic groups have been investigated as bioindicators due to their distinctive attributes, including ease of sampling, differential responses to disturbances, associations with species richness, and accessible taxonomy. Objective: To compare the potential of four taxa (Chilopoda, Scarabaeidae, Carabidae, and Pompilidae) as environmental bioindicators, considering their ease of collection, presence of changes in community composition due to disturbances, including species with preferences for disturbed or conserved areas; and to correlate their diversity with the diversity of other taxa. Methods: The study was conducted at the Manu Learning Centre Biological Station in the Manu Biosphere Reserve, which comprises three areas along a disturbance gradient. Specimens were collected using pitfall traps, yellow pan traps, Malaise traps, and monoliths from May to June in 2021. Ease of collection was measured in terms of richness and abundance; changes in the community composition were assessed using similarity analysis, non-metric multidimensional scaling, and beta diversity partitioning; presence of species with preferences was analyzed using correspondence analysis and G. Williams’ goodness-of-fir test; and diversity correlation using the Spearman index. Results: Among the taxa investigated, Scarabaeidae and Pompilidae were the easiest to collect. Changes in composition were observed in all taxa but were significant only in Chilopoda and Scarabaeidae. All taxa exhibited species with preferences for disturbed and undisturbed environments. There was a high correlation in diversities among Carabidae, Scarabaeidae, and Chilopoda. Conclusions: Our findings underscore the diverse responses exhibited by taxa along a disturbance gradient. Notably, Scarabaeidae and Chilopoda emerged as particularly robust bioindicators within the studied ecosystem.

Resumen Introducción: El monitoreo de bioindicadores es una herramienta importante para la conservación de áreas naturales protegidas. Diferentes grupos taxonómicos fueron estudiados como bioindicadores por presentar: facilidad de muestreo, respuestas variadas a la perturbación, riqueza asociada a otras especies, y taxonomía accesible. Objetivo: Comparar el potencial de cuatro taxones (Chilopoda, Scarabaeidae, Carabidae y Pompilidae) como bioindicadores ambientales, tomando en cuenta su facilidad de recolección, presencia de cambios en la composición de sus comunidades por perturbaciones, presencia de especies con preferencia por áreas perturbadas o conservadas; y diversidad correlacionada con la diversidad de los otros taxones. Métodos: El trabajo se realizó en la estación Biológica Manu Learning Centre en la Reserva de Biosfera del Manu, que comprende tres áreas en un gradiente de perturbación. Los ejemplares fueron recolectados con trampas pitfall, bandejas amarillas, trampas Malaise y monolitos, de mayo a diciembre del 2021. La facilidad de recolección se midió en términos de riqueza y abundancia; los cambios en la composición de sus comunidades mediante el análisis de similitud, escalamiento no métrico multidimensional, y partición de la diversidad beta; presencia de especies con preferencia con el análisis de correspondencia y el ajuste de bondad G de Williams; y finalmente la correlación de las diversidades con el índice de Spearman. Resultados: De los taxones estudiados, las de más fácil recolección fueron Scarabaeidae y Pompilidae. Existen cambios en la composición en todos los taxones, pero son importantes solo en Chilopoda y Scarabaeidae. En todos los taxones hay especies con preferencia por ambientes perturbados y no perturbados. Existe una alta correlación de las diversidades de Carabidae, Scarabaeidae y Chilopoda. Conclusiones: Todos los grupos responden de diferente forma a los gradientes de perturbación, Scarabaeidae y Chilopoda fueron los que tuvieron mejores características para ser considerados buenos bioindicadores.

Abstract Introduction: Gorse (Ulex europaeus, family Fabaceae) is an evergreen shrub native to Europe and invasive in the Andean high-mountain tropical paramos. Objective: To quantify the extent of a biological invasion within a paramo near Los Nevados National Natural Park in Tolima, Colombia, and evaluate bioeconomic solutions to encourage shrub eradication while promoting the local economy and industry. Methods: An object-based supervised classification approach was conducted using UAS (Uncrewed Aircraft Systems)-based RGB imagery and a Planet-derived NDVI (Normalized Difference Vegetation Index) layer, both from 2022, to quantify the area invaded. This value, the height obtained from a UAS-derived nDSM (Normalized Digital Surface Model), and a pair of allometric equations found in the literature were employed to estimate the gorse aboveground biomass (AGB) and aboveground available fuel, also known as fuel load. Then, documented bioethanol production estimations were applied to calculate its potential extraction based on the AGB values. Results: The invaded area was quantified to be 66 465 m2, with an overall accuracy rate of 85.3 %. Furthermore, the fuel load was found to be approximately half of the AGB, which poses a high risk of fire in the ecosystem. The findings also suggest that up to USD 88 933.7 could be generated if all gorse in the study area is exploited to produce bioethanol. Conclusions: This study underscores the urgency of managing the biological invasion of gorse in the Andean paramo and suggests the potential for bioeconomic solutions to mitigate the impact of these invasions.

Resumen Introducción: El retamo espinoso (Ulex europaeus, familia Fabaceae) es un arbusto perenne originario de Europa e invasor en los páramos andino-tropicales de alta montaña. Objetivo: Cuantificar la extensión de una invasión biológica dentro de un páramo cerca del Parque Nacional Natural Los Nevados en Tolima, Colombia, y evaluar soluciones bioeconómicas para fomentar la erradicación del arbusto y, al mismo tiempo, promover la economía e industria locales. Métodos: Se llevó a cabo una clasificación supervisada basada en objetos utilizando imágenes RGB tomadas con UAS (sistemas de aeronaves no tripulados) y una capa NDVI (índice de vegetación de diferencia normalizada) derivada de Planet, ambas de 2022, para cuantificar el área invadida. Este valor, así como la altura obtenida a partir de un nDSM (modelo digital de superficie normalizado) derivado de las imágenes UAS y un par de ecuaciones alométricas halladas en la literatura, se emplearon para estimar la biomasa aérea (AGB) y el combustible disponible superficial, también conocido como carga de combustible. Luego, se utilizaron estimaciones documentadas de producción de bioetanol para calcular su potencial extracción con base en los valores de AGB. Resultados: El área invadida se cuantificó en 66 465 m2, con una exactitud global del 85.3 %. Además, se encontró que la carga de combustible era aproximadamente la mitad del AGB, lo que representa un alto riesgo de incendio en el ecosistema. Los resultados también sugieren que se podrían generar hasta 88 933.7 dólares estadounidenses, si se explotara todo el retamo espinoso de la zona de estudio para producir bioetanol. Conclusiones: Este estudio resalta la urgencia de gestionar la invasión biológica del retamo espinoso en el páramo andino y sugiere potenciales soluciones bioeconómicas para mitigar el impacto de estas invasiones.

Abstract Introduction: The River Tambo basin is one of the main hydrographic systems of the Peruvian western watershed, being a source of development for agriculture, agroindustry, livestock, and domestic use in the Tambo Valley and Arequipa City. Objective: To analyze the structure of macroinvertebrates in River Tambo, Perú and assess the water quality through this community. Methods: Five sampling events were conducted between December 2018 and November 2019 in 12 stations, using a Surber net (500 μm). Macroinvertebrates were identified at a family level. To assess community structure, richness, Simpson’s dominance index (D), Pielou’s evenness (J’), and true diversity of order 1 were determined. A nMDS based on the Bray-Curtis index was used to evaluate dissimilarity. To check for differences in community structure, ANOSIM and two-way MANOVA were used. SIMPER was used to establish family contribution to sample similarity. Relations between physicochemical and biological variables were determined by CCA. ABI index was applied to assess ecological quality. Results: 32 families were recognized, being the most abundant: Baetidae, Chironomidae, Leptohyphidae, Simuliidae, Hydroptilidae, and Elmidae. The middle and low zones of the basin showed the highest and lowest diversity respectively. There were significant differences (P < 0.05) in the community structure indices among the 12 stations differed. There was a higher similarity among the 12 stations in December. ABI scores increased from the low to the middle part of the river, which showed the highest ecological quality. Conclusion: Macroinvertebrate communities from River Tambo reflect ecosystem conditions. Environments impacted by human activities show lower diversity and ecological quality, due to the structure, habitat, and water quality of the river being altered.

Resumen Introducción: La cuenca del rio Tambo es uno de los principales sistemas hidrográficos de la vertiente occidental del Perú y fuente de desarrollo para la agricultura, agroindustria, ganadería y consumo doméstico en el valle de Tambo y la ciudad de Arequipa. Objetivo: Analizar la estructura de macroinvertebrados en el río Tambo, Perú y evaluar la calidad del agua a través de esta comunidad. Métodos: Entre diciembre del 2018 y noviembre del 2019 se realizaron cinco muestreos en 12 estaciones, empleando una red Surber (500 μm). Los macroinvertebrados se identificaron a nivel de familia. Se registró la riqueza, dominancia de Simpson, equidad de Pielou, y diversidad verdadera de orden 1, para evaluar la estructura de la comunidad. Para estimar la similitud, se utilizó nMDS (índice de Bray-Curtis). Para verificar si existen diferencias en la estructura comunitaria, se empleó MANOVA de dos vías y ANOSIM. Para establecer la contribución de las familias a la similitud entre muestras, se utilizó SIMPER. La relación entre variables fisicoquímicas y biológicas se determinó con ACC. Se aplicó el índice ABI para evaluar la calidad ecológica. Resultados: se reconocieron 32 familias, siendo las más abundantes: Baetidae, Chironomidae, Leptohyphidae, Simuliidae, Hydroptilidae y Elmidae. La mayor y menor diversidad se presentó en la parte media y baja de la cuenca respectivamente. Existieron diferencias significativas (P < 0.05) de los índices de estructura comunitaria entre las 12 estaciones. Se evidenció mayor similitud entre las 12 estaciones en diciembre. Los puntajes del índice ABI se incrementan de la parte baja del río a la media, que se presentó la mejor calidad ecológica. Conclusión: Las comunidades de macroinvertebrados del río Tambo reflejan el estado del ecosistema. Los ambientes con impacto por actividad humana registran menor diversidad y calidad ecológica, ya que se altera la estructura, hábitat y calidad de agua del río.

ABSTRACT Maize (Zea mays L.) is highly sensitive to salt stress, which poses a threat to its production, especially with predicted increases in salinity due to climate change. Enhancing salt tolerance in maize is critical to mitigating these adverse effects, sustaining its production, and ensuring global food security. This study aimed to evaluate the effects of exogenously applied lipoic acid or Si, alone or in integration with soil application of vermicompost-tea on physio-biochemical components, antioxidant defense systems, growth, and yield of maize plants exposed to salt stress. The application of vermicompost-tea was carried out as a soil treatment incorporated into irrigation water at a concentration of 200 L ha-1, and administered in three equal doses in irrigation water within the last 10 min of drip irrigation. Silicon was applied at a rate of 6 mM using potassium silicate and lipoic acid was applied at 0.1 mM. Silicon and lipoic acid were applied three times at 25, 40, and 55 d after planting. The results indicated that the integrative application of soil-based vermicompost-tea with exogenously applied Si or lipoic acid significantly enhanced all studied parameters. Specifically, the combination of Si with vermicompost-tea exhibited the highest improvement percentage compared to untreated control in chlorophyll a (60.3%), chlorophyll b (72.7%), carotenoids (69.9%), net photosynthetic rate (111%), transpiration rate (134%), stomatal conductance (129.5%), relative water content (28.4%), membrane stability index (44.3%), N (33.4%), P (71.9%), K (39.7%), Ca (89.9%), K/Na (81.2%), total soluble sugars (63.7%), proline (60.1%), ascorbate (44.4%), glutathione (83.5%), and α-tocopherol (72.1%). These positive effects were reflected in enhancing yield traits under salt-affected soil conditions by enhancing plant height (25.1%), number of grains per row (43.3%), number of rows per ear (26.7%), 1000‐grain weight (25.9%), grain yield (45.4%), and biological yield (42.8%) compared to untreated control.

Abstract Introduction: Neanderthals, our extinct relatives, exhibited distinct nasal morphology compared to Homo sapiens. Objectives: This study explores these differences through a comparative analysis of cranionasal measurements. Material and Methods: Data on Neanderthal nasal features were obtained from existing paleoanthropological literature, focusing on 16 hominid fossils. A parallel sample of 38 modern human CT scans was retrospectively reviewed, with measurements focusing on nasal region. Results: Neanderthals displayed significantly larger nasal cavities with increased basion-nasion and basion-prosthion distances, wider intercanine dimensions, and larger nasal breadth and height compared to modern humans. Choanal width and nasal index did not differ significantly. Conclusions: These findings suggest functional adaptations in Neanderthal nasal morphology, potentially aiding in thermoregulation within cold environments. Further research is needed to fully understand the evolutionary significance of these adaptations.

Resumo Introdução: Os Neandertais, nossos parentes extintos, exibiam uma morfologia nasal distinta em comparação com o Homo sapiens. Objetivos: Este estudo explora essas diferenças através de uma análise comparativa de medidas nasocranianas. Material e Métodos: Dados sobre as características nasais do Homo Neanderthalensis e Homo Heilderbergensis foram obtidos a partir da literatura paleoantropológica existente, com foco em 16 fósseis. Uma amostra paralela de 38 de crânios de Homo Sapiens foi analisada retrospetivamente mediante tomografia computadorizada (TC), com obtenção de medições relativas à região nasal. Resultados: Os Hominídeos exibiram cavidades nasais significativamente maiores, com distâncias basion-nasion e basion-prosthion aumentadas, dimensões intercaninas maiores e maior largura e altura nasal em comparação com os humanos modernos. A largura coanal e o índice nasal não diferiram significativamente. Conclusões: Esses achados sugerem adaptações funcionais na morfologia nasal neandertal, potencialmente auxiliando na termorregulação em ambientes frios. Pesquisas adicionais são necessárias para compreender totalmente o significado evolutivo dessas adaptações.

Resumo O desenvolvimento humano é influenciado pela interação entre fatores biológicos e sociais. Este estudo teve como objetivo verificar o efeito moderador do risco social na relação entre risco biológico e desenvolvimento infantil. Os dados foram coletados em 201 crianças, com idades entre 6 e 72 meses. A variável independente foi medida pelo índice de risco biológico, e a variável moderadora pelo índice de risco social. O desenvolvimento infantil foi avaliado por meio do teste Denver II. Regressão linear, tamanho do efeito e análise de moderação foram utilizados para verificar a relação entre IRB e o desenvolvimento infantil (Denver II) e o efeito moderador do IRS. O IRB associou-se negativamente ao desenvolvimento infantil, a interação entre o IRB e o IRS aumentou a variância explicada no resultado do Denver II para 14%. O IRS também foi um moderador significativo dos domínios Linguagem e Motor Grosso. Esta pesquisa evidenciou que o risco social é um moderador da relação entre risco biológico e desenvolvimento infantil; quanto mais fatores de risco social, mais forte essa relação se torna. Por outro lado, pode-se dizer que alguns fatores sociais favorecem o desenvolvimento infantil, mesmo na presença de fatores de risco biológicos. 20 crianças 7 meses linear associouse associou 14 14% Grosso torna lado podese pode 2 1

Abstract Human development is influenced by the interaction between biological and social factors. This study aimed to verify the moderating effect of social risk on the relationship between biological risk and child development. Data were collected on 201 children, aged 6 to 72 months. The independent variable was measured by the biological risk index, and the moderator variable by the social risk index was assessed by the Denver II test. Linear regression, effect size, and analysis of moderation were used to verify the relationship between BRI and the child development (Denver II), and the moderating effect of the SRI. BRI was negatively associated with child development, the interaction between the BRI and SRI increased the explained variance in the Denver II result to 14%. The SRI was also a significant moderator of the Language and Gross Motor domains. This research evidence that social risk moderates the relationship between biological risk and child development, the more social risk factors, the stronger this relationship becomes. On the other hand, it can be said that some social factors favor child development, even in the presence of biological risk factors. 20 children 7 months test regression size II, , II) 14 14% domains becomes hand 2 1

Abstract Objectives: Age at menarche (MA) is a proxy for biological maturation and a parameter of socioeconomic changes. Worldwide, anticipation of menarche is associated with nutritional transition and excess weight. The objective of this study was to evaluate the MA in Amazonian students and its association with nutritional status, ethnicity, and socioeconomic level. Methods: Cross-sectional study with 1,017 students aged 6 to 17 living in the city of Manaus, Brazil. MA was analyzed by status quo and recall; its association with body mass index (BMI), race, socioeconomic status, and adult height was examined. Results: 559 (51.9%) participants had already experienced menarche. In 91.7%, menarche occurred between 10 and 14 years of age; the mean age at the onset of menarche was 11.9 years. Overweight (11.6 years) and obese (11.4 years) participants reached menarche earlier than those with normal weight (12 years) and lean (12.7 years) participants. The associations between MA and nutritional status showed that overweight and obesity are risk factors for the early occurrence of menarche. MA was not associated with socioeconomic status/parental education or race. However, excess weight was associated with earlier MA in all races and social classes. The adult height was slightly lower in girls with menarche before 12 years old (157.9 vs 159.4 cm). Conclusion: Regardless of socioeconomic level or ethnicity, excess weight was associated with earlier menarche in Amazonian students. Objectives (MA changes Worldwide ethnicity Methods Crosssectional Cross sectional 1017 1 017 1,01 Manaus Brazil recall BMI, BMI , (BMI) race examined Results 55 51.9% 519 51 9 (51.9% 917 91 7 91.7% 119 11 11. 11.6 116 (11. 11.4 114 4 (1 12.7 127 (12. statusparental parental However classes 157.9 1579 157 (157. 1594 159 159. cm. cm . cm) Conclusion 101 01 1,0 (BMI 5 51.9 (51.9 91.7 (11 ( 12. 157. 15 (157 0 1, 51. (51. 91. (15 (51 (5

Resumen Esta es una investigación base en la que se planteó el objetivo de estimar el Índice Aproximado de Sostenibilidad (IAS) en La Paz, San Ramón, Costa Rica. Para recopilar datos se aplicó un censo habitacional entre agosto de 2016 y marzo de 2017. El resultado del IAS fue 0,7856. los resultados reflejaron que en ocho indicadores poseen condición como muy aceptable, tres indicadores resultaron aceptable, dos indicadores en condición regular y uno de los indicadores se encuentra en condición totalmente inaceptable. El parámetro social obtuvo el índice parcial más bajo con 0,583, los parámetros ambiental y económico comparten un valor de 0,875, mientras el parámetro institucional alcanzó 0,75. La investigación marca un parámetro de partida para futuros estudios que permitan monitorear la sostenibilidad en la zona.

Abstract This is a base investigation in which the objective of estimating the Approximate Sustainability Index (IAS) in La Paz, San Ramón, Costa Rica was set. To collect data, a housing census was applied between August 2016 and March 2017. The IAS result was 0.7856. The results reflected that eight indicators have a very acceptable condition, three indicators were acceptable, two indicators in a regular condition and one of the indicators is in a totally unacceptable condition. The social parameter obtained the lowest partial index with 0.583, the environmental and economic parameters share a value of 0.875, while the institutional parameter reached 0.75. The research marks a starting parameter for future studies that allow monitoring sustainability in the area.

Abstract Background: Older adults have highly heterogeneous aging rates. Objective: To explore the association of biological age (BA) and accelerated aging with frailty in community-dwelling older adults. Material and methods: We assessed 735 community-dwelling older adults from the Coyocan Cohort. BA was measured using AnthropoAge, accelerated aging with AnthropoAgeAccel, and frailty using Fried’s phenotype and the frailty index. We explored the association of BA and accelerated aging (AnthropoAgeAccel ≥ 0) with frailty at baseline and characterized the body composition and physical function phenotype of accelerated aging in non-frail/frail participants. We also explored accelerated aging as a risk factor for frailty progression after 3-years of follow-up. Results: Older adults with accelerated aging have higher frailty prevalence and indices, lower handgrip strength and gait speed. AnthropoAgeAccel was associated with higher frailty indices (β = 0.0053, 95 % CI = 0.0027-0.0079), and increased odds of frailty at baseline (OR = 1.16, 95 % CI = 1.09-1.25). We observed sex-based differences in body composition and physical function linked to accelerated aging in non-frail participants; however, these differences were absent in pre-frail/frail participants. Accelerated aging at baseline was associated with higher risk of frailty progression over time (OR = 1.74, 95 % CI = 1.11-2.75). Conclusions: Despite being intertwined, biological accelerated aging is largely independent of frailty in community-dwelling older adults.

Resumen Antecedentes: Los adultos mayores tienen tasas de envejecimiento heterogéneas. Objetivo: Explorar la asociación entre edad biológica (EB) y envejecimiento acelerado (EA) con fragilidad en adultos mayores. Material y métodos: Análisis de 735 adultos mayores de la Cohorte de Coyocán. Se estimó EB con AnthropoAge, EA con AnthropoAgeAccel y fragilidad con el fenotipo de Fried y el índice de fragilidad (IF). Se exploró la asociación de EB y EA (AnthropoAgeAccel ≥ 0) con fragilidad; se caracterizó su presencia simultánea sobre fenotipos de composición corporal y función física. Se determinó el riesgo de EA para progresión del fenotipo de fragilidad a tres años de seguimiento. Resultados: Los adultos mayores con EA presentaron mayor prevalencia de fragilidad e IF, menor fuerza de prensión y velocidad de marcha. Los resultados de AnthropoAgeAccel se asociaron a IF (β = 0.0053, IC 95 % = 0.0027-0.0079) y al fenotipo de fragilidad (RM = 1.16, IC 95 % = 1.09-1.25). Existieron diferencias por sexo en composición corporal y función física relacionadas con EA solo en participantes no frágiles. El EA en la evaluación basal se asoció con progresión de la fragilidad con el tiempo (RM = 1.74, IC 95 % = 1.11-2.75). Conclusiones: A pesar su asociación, el EA es independiente de la fragilidad en adultos mayores que viven en la comunidad.

Abstract Introduction: Biohacking's emergence has reshaped scientific research, especially within transhumanism and biological manipulation. Evaluating its impact on the scientific community is crucial for defining future research directions and communication in this field. Objective: To critically examine perspectives, approaches, and debates in academic literature to develop a comprehensive understanding of the ethical, social, and cultural implications of technological advancement within the transhumanist context. Methods: SCOPUS and bibliometric analysis were employed to quantify biohacking's influence. Social Network Analysis identified key themes and research focus areas. Results: North American and European countries led in scientific productivity. Published articles and citation impact showed a moderate to low index. Social Network Analysis highlighted ethical considerations, social implications, and an emerging discourse on cognitive evolution. Conclusion: The results highlight the need for comprehensive education and communication strategies to ensure that the public is well-informed about the risks, benefits, and ethical considerations associated with genetic modification technologies. Therefore, more research on legislation and regulations is required.

Resumen Introducción: La aparición del biohacking ha reconfigurado la investigación científica, especialmente en el ámbito del transhumanismo y la manipulación biológica. Evaluar su impacto en la comunidad científica es crucial para definir futuras direcciones de investigación y comunicación en este campo. Objetivo: Examinar críticamente perspectivas, enfoques y debates en la literatura académica para desarrollar una comprensión global de las implicaciones éticas, sociales y culturales del avance tecnológico dentro del contexto transhumanista. Métodos: Se empleó la base de datos SCOPUS y análisis bibliométricos para cuantificar la influencia del biohacking. El análisis de redes sociales identificó temas clave y áreas de investigación. Resultados: Los países norteamericanos y europeos lideraron la productividad científica. Los artículos publicados y el impacto de las citas mostraron un índice de moderado a bajo. El análisis de redes sociales puso de relieve consideraciones éticas, implicaciones sociales y un discurso emergente sobre la evolución cognitiva. Conclusión: Los resultados ponen de relieve la necesidad de estrategias integrales de educación y comunicación para garantizar que el público esté bien informado sobre los riesgos, beneficios y consideraciones éticas asociados a las tecnologías de modificación genética. Por lo tanto, se requiere más investigación sobre legislación y normativa.

Resumo Introdução: A emergência do biohacking veio remodelar a investigação científica, nomeadamente no domínio do transhumanismo e da manipulação biológica. A avaliação do seu impacto na comunidade científica é crucial para definir futuras direcções de investigação e comunicação neste domínio. Objetivo: Examinar criticamente perspectivas, abordagens e debates na literatura académica para desenvolver uma compreensão abrangente das implicações éticas, sociais e culturais do avanço tecnológico no contexto transhumanista. Métodos: A base de dados SCOPUS e a análise bibliométrica foram utilizadas para quantificar a influência do biohacking. A análise de redes sociais identificou os principais temas e áreas de investigação. Resultados: Os países da América do Norte e da Europa lideraram a produtividade científica. Os artigos publicados e o impacto das citações revelaram uma taxa moderada a baixa. A análise da rede social destacou considerações éticas, implicações sociais e um discurso emergente sobre a evolução cognitiva. Conclusão: Os resultados sublinham a necessidade de estratégias abrangentes de educação e comunicação para garantir que o público esteja bem informado sobre os riscos, benefícios e considerações éticas associadas às tecnologias de modificação genética. Por conseguinte, é necessária mais investigação sobre legislação e regulamentação.

ABSTRACT Understanding the dietary needs is important for the conservation of the bighorn sheep. The objective was to identify the diet selection in Coahuila, Mexico. The research was conducted at the Rancho San Juan Wildlife Management Unit (UMA), Monclova municipality, from August 2018 to October 2019. Forage availability was assessed using 18 Canfield transects. Diet composition was estimated through microhistology on 280 fecal samples, and diet and forage diversity were assessed using the Shannon index. Cluster and principal component analyses (PCA) were applied to analyze similarity in consumption across different species and seasonal influences. Shrubs were the most available (48%). A total of 50 species and 14 families were identified in the diet, with shrubs (49.2%) and grasses (17.21%) being predominant. Tiquilia canescens, Gymnosperma glutinosum, Opuntia rufida, Medicago sativa, and Erioneuron pulchellum were the most consumed species. The bighorn sheep consumed tree and shrub species proportionally (1.1 and 1.02, respectively), preferred herbaceous plants in spring (2.24) and autumn (15.28), and grasses in spring (6.10), except in summer (0.83) when they avoided succulents. PCA revealed that the first two components explained 96.05% of the correlation between species consumption frequencies and their biological forms. Cluster analysis grouped five forage consumption categories. Conserving shrub species, which form the basis of the bighorn sheep's diet, grasses-its preference-and succulents as buffering elements, is essential.

RESUMEN Comprender las preferencias alimentarias es importante para la conservación del borrego cimarrón. El objetivo fue identificar la selección de su dieta en Coahuila, México. La investigación se realizó en la UMA Rancho San Juan, municipio de Monclova, de agosto 2018 a octubre 2019. La disponibilidad de forraje se evaluó con 18 líneas de Canfield. La composición de la dieta se estimó con microhistología en 280 muestras fecales, y la diversidad de la dieta y del forraje con el índice de Shannon. Para analizar la similitud en el consumo de diferentes especies y la influencia estacional, se aplicaron análisis de clústers y componentes principales (ACP). Las arbustivas estuvieron más disponibles (48%). Se identificaron 50 especies y 14 familias en la dieta, y predominaron las arbustivas (49.2%) y los pastos (17.21%). Tiquilia canescens, Gymnosperma glutinosum, Opuntia rufida, Medicago sativa y Erioneuron pulchellum fueron las más consumidas. El borrego consumió especies arbóreas y arbustivas de manera proporcional (1.1 y 1.02, respectivamente), prefirió las herbáceas en primavera (2.24) y otoño (15.28), los pastos en primavera (6.10), y con excepción de verano (0.83), evitó las suculentas. El ACP reveló que los dos primeros componentes explican el 96.05% de la correlación entre las frecuencias de consumo de las especies y sus formas biológicas. El análisis de clústers agrupó cinco categorías de consumo de forraje. Es fundamental conservar las arbustivas, que constituyen la base de la dieta del borrego cimarrón, los pastos, que son sus preferidos, y las suculentas, como elementos amortiguadores.

Abstract This study aimed to evaluate whether bacterial inoculation improves the fermentative, microbiological, and chemical characteristics of silages of the elephant grass cv. BRS Capiaçu on different regrowth days. The experimental design was completely randomized and set up in a 3x2 factorial arrangement (three regrowth days, with and without inoculant), with four replications. There was a significant interaction between the regrowth days and inoculant on the pH, ammoniacal nitrogen (N-NH3), and effluent losses (EL) of the silages. Inoculation decreased the EL with the advance of regrowth days and increased the dry matter recovery index compared to the silages without inoculant. The population of molds and yeasts decreased when inoculation was adopted to the forage harvested after 85 d. There was a significant interaction between the dry matter (DM), crude protein (CP) and neutral detergent fiber corrected for ash and protein (NDFap) contents of the silages. Inoculation in the grass harvested after 85 d increased the DM contents of the silage. The highest CP contents were observed in the silages after 85 d. The NDFap contents of the grasses harvested after 110 and 135 d were higher than those of the grass harvested after 85 d. The NDFap contents of the silages without inoculant increased with the harvest age. The BRS Capiaçu forage silage harvested at 110 d demonstrated favorable performance for silage production. However, the influence of inoculant use was low for the characteristics evaluated.

Resumen Este estudio tuvo como objetivo evaluar si la inoculación bacteriana mejora las características fermentativas, microbiológicas y químicas de los ensilados del pasto elefante cv. BRS Capiaçu en diferentes días de rebrote. El diseño experimental fue completamente al azar y se estableció en un arreglo factorial 3x2 (tres días de rebrote, con y sin inoculante), con cuatro repeticiones. Hubo una interacción significativa entre los días de rebrote y el inoculante sobre el pH, el nitrógeno amoniacal (N-NH3) y las pérdidas por efluentes (PE) de los ensilados. La inoculación disminuyó la PE con el avance de los días de rebrote y aumentó la tasa de recuperación de materia seca en comparación con los ensilados sin inoculante. La población de mohos y levaduras disminuyó cuando se adoptó la inoculación al forraje cosechado después de 85 d. Hubo una interacción significativa entre la materia seca (MS), la proteína cruda (PC) y la fibra detergente neutro corregida por los contenidos de cenizas y proteína (FDNcp) de los ensilados. La inoculación en el pasto cosechado después de 85 días aumentó el contenido de MS del ensilado. Los mayores contenidos de PC se observaron en los ensilados después de 85 días. Los contenidos de FDNcp de los pastos cosechados después de 110 y 135 días fueron mayores que el del pasto cosechado después de 85 días. El contenido de FDNcp de los ensilados sin inoculante aumentó con la edad de cosecha. El ensilado de forraje de BRS Capiaçu cosechado a los 110 días demostró un desempeño favorable para la producción de ensilado. Sin embargo, la influencia del uso de inoculantes fue baja para las características evaluadas.

Resumo Introdução: a escassez de debates sobre o cuidado de enfermagem às gestantes com doença falciforme no país e a urgente necessidade de intervenção e melhorias na assistência de enfermagem prestada às essas mulheres, motivaram este estudo. Além disso, em instrumentos para uso em pesquisa e/ou na prática clínica, a validade é um atributo essencial. Objetivo: desenvolver e validar um instrumento para cuidados às gestantes com doença falciforme. Materiais e método: estudo metodológico, de validação de conteúdo com juízas(es) especialistas. O instrumento foi estruturado com 19 diagnósticos de enfermagem que abordaram as dimensões biológica, psíquica e social, e 126 intervenções de enfermagem. Utilizou-se a técnica Delphi, com participação de 18 juízas(es). Os resultados foram analisados por meio do índice de validade de conteúdo e nível de concordância acima de 0,80. Resultados: dos 145 itens analisados, 22 (15,17 %) apresentaram índice de validade de conteúdo < 0,80, e os ajustes sugeridos pelas(os) juízas(es) foram implementados. O índice de validade de conteúdo geral do instrumento foi calculado em 0,87. O instrumento alcançou parámetros aceitáveis de validade de conteúdo, segundo o critério utilizado. Conclusões: destaca-se o potencial do instrumento, que pode ser aprimorado a partir do seu uso, como recurso na orientação das práticas de enfermagem voltadas às mulheres grávidas com doença falciforme, nos âmbitos da atenção primária à saúde e da rede hospitalar.

Resumen Introducción: la escasez de debates sobre la atención de enfermería a gestantes con anemia de células falciformes en el país y la urgente necesidad de intervención y mejora de los cuidados de enfermería prestados a esas mujeres motivaron la realización de este estudio. Además, la validez es un atributo esencial en los instrumentos para uso en investigación y/o práctica clínica. Objetivo: elaborar y validar un instrumento de atención a gestantes con anemia de células falciformes. Material y método: estudio metodológico, de validación de contenido con jueces/as expertos/as. El instrumento se estructuró con 19 diagnósticos de enfermería que abordaban las dimensiones biológica, psicológica y social, y 126 intervenciones de enfermería. Se utilizó la técnica Delphi, con la participación de 18 jueces/as. Los resultados se analizaron utilizando el índice de validez de contenido y un nivel de acuerdo superior a 0,80. Resultados: de los 145 ítems analizados, 22 (15,17 %) tenían un índice de validez de contenido < 0,80, y se aplicaron los ajustes sugeridos por los/las jueces/as. El índice global de validez de contenido del instrumento se calculó en 0,87. El instrumento alcanzó parámetros de validez de contenido aceptables, según los criterios utilizados. Conclusiones: se destaca el potencial del instrumento, que puede ser perfeccionado a través de su utilización como recurso para orientar las prácticas de enfermería dirigidas a gestantes con anemia de células falciformes, tanto en la atención primaria de salud como en la red hospitalaria.

Abstract Introduction: The lack of discussions on nursing care for pregnant women with sickle cell disease in Brazil and the urgent need for intervention and improvements in the nursing care provided to these women sparked this study. In addition, validity is an essential attribute for instruments used in research and/or clinical practice. Objective: To develop and validate an instrument covering the care of pregnant women with sickle cell disease. Materials and methods: This is a methodological, content validation study conducted with specialist judges. The instrument was structured with 19 nursing diagnoses that addressed the biological, psychological, and social dimensions, as well as 126 nursing interventions. The Delphi technique was used with the participation of 18 judges. The results were analyzed using the content validity index and a level of agreement above 0.80. Results: Of the 145 items analyzed, 22 (15.17 %) scored a content validity index < 0.80, and the adjustments suggested by the judges were implemented. The instrument’s general content validity index was calculated at 0.87. The instrument reached acceptable content validity parameters, according to the criteria used. Conclusions: The instrument’s potential stands out, and it can be improved through its use as a resource for guiding nursing practices directed to pregnant women with sickle cell disease, both in primary health care and in hospital networks.

ABSTRACT The aim of this study was to evaluate the biological, physicochemical and technological related characteristics of the blue shark Prionace glauca. The sample included 128 specimens to determine their biological and technological properties and 27 specimens for their physicochemical characteristics. Biometric data of length and weight (142 to 281 cm and 10.5 to 93.0 kg for males; 153.5 to 255.0 cm and 14.5 to 80.0 kg for females), allometry index (males 3.27 and females 2.87), technological potential through different types of performance (RED: 83.9±0.03%, RESV: 67.2±0.04%, RSCV: 48.9±0.07% and RPC: 33.5±0.07%) and water retention capacity in the muscle (16.1±0.03%). Within the physicochemical characteristics, the proximal chemical composition was analysed (protein 21.8±0.23; fat 0.6±0.03; humidity78.0±0.31; ash 1.3±0.01) and mineral composition (Cu: 2.0±0.11 mg/kg; Fe: 17.8±1.95 mg/kg; Zn: 25.8±1.76 mg/kg; Ca: 4.3±0.27 mg/100 g; Mg:95.4±7.87 mg/100g;Na:716.3±24.03mg/100g;K:1526.1±54.4mg/100g;P:439.5±15.76 mg/100 g). There was no evidence of significant differences based on sex.

RESUMEN El objetivo del estudio fue evaluar las características biológicas, fisicoquímicas y tecnológicas relacionadas al tiburón azul Prionace glauca. Se trabajó con 128 especímenes para determinar sus características biológicas, tecnológicas y 27 ejemplares para evaluar la composición fisicoquímica de su carne. Se registraron los datos biométricos de longitud y peso (142 a 281 cm y 10.5 a 93.0 kg para machos; 153.5 a 255.0 cm y 14.5 a 80.0 kg para hembras), índice de alometría (machos 3.27 y hembras 2.87), potencial tecnológico mediante diferentes tipos de rendimientos (RED: 83.9±0.03%, RESV: 67.2±0.04%, RSCV: 48.9±0.07% y RPC: 33.5±0.07%) y capacidad de retención de agua en el músculo (16.1±0.03%). Dentro de las características fisicoquímicas se analizó la composición química proximal (proteína 21.8±0.23; grasa 0.6±0.03; humedad 78.0±0.31; ceniza 1.3±0.01) y los minerales (Cu: 2.0±0.11 mg/kg, Fe: 17.8±1.95 mg/kg, Zn: 25.8±1.76 mg/kg; Ca: 4.3±0.27 mg/100 g, Mg: 95.4±7.87 mg/100 g, Na: 716.3±24.03 mg/100 g, K: 1526.1±54.4 mg/100 g, P: 439.5±15.76 mg/100 g). No hubo evidencia de diferencias significativas en función al sexo. Respecto a las comparaciones realizadas con bases de datos de recursos pesqueros, el músculo de tiburón azul no presenta ventajas comparativas relativos al rendimiento, perfil mineral o composición química proximal de otras especies.