RESUMO Este estudo foca no desempenho de vacas para produção de carne, criadas nos pântanos do Paraguai, examinando cinco genótipos bovinos; Brahman, Brangus, Nelore, bem como duas raças locais em risco de extinção. O principal objetivo é identificar e classificar variáveis fenotípicas que incluem variáveis sanguíneas, clínicas, de pelagem e saúde, demonstrando ligação causal com o peso vivo das vacas analisadas. Inicialmente, foram identificadas correlações elevadas entre diferentes variáveis incluídas neste estudo, e, então, utilizando técnicas avançadas de aprendizado de máquina e a aplicação de explicações aditivas de Shapley (SHAP), foi proporcionado um entendimento mais profundo dos fatores fortemente associados à adaptabilidade nestes ambientes, e, portanto, o respectivo desempenho zootécnico. A associação entre o componente genotípico bovino ligado à estação do ano mostrou ser o fator influente mais predominante sobre o peso vivo bovino. Variáveis como comprimento do pelo, hematócrito, fosfatase, fósforo, creatina phosphokinase, creatinina, proteína, cortisol, cálcio e a presença de endoparasitas foram destacadas, demonstrando sua importância hierárquica para a seleção animal. Os modelos de ML são ferramentas eficazes para estabelecer hierarquias de relevância em multivariáveis fenotípicas complexas, o que é crucial em programas de melhoramento genético em diferentes espécies zootécnicas, bem como em ambientes especiais e específicos, como os pântanos. carne Paraguai bovinos Brahman Brangus Nelore extinção sanguíneas clínicas saúde analisadas Inicialmente então SHAP, SHAP , (SHAP) portanto zootécnico pelo hematócrito fosfatase fósforo phosphokinase creatinina proteína cortisol destacadas animal complexas zootécnicas específicos (SHAP

ABSTRACT This study focuses on the performance of cows for meat production raised in the wetlands of Paraguay, examining five cattle genotypes: Brahman, Brangus, and Nelore, as well as two local breeds at risk of extinction. The main objective is to identify and rank phenotypic variables, including blood, clinical, hair, and health variables, demonstrating causal linkage with the live weight of the cows analyzed. Initially, high correlations were identified between different variables included in this study; then, using advanced Machine learning (ML) techniques and the application of Shapley additive explanations (SHAP), a deeper understanding was provided of the factors strongly associated with adaptability in these environments, and, therefore, the respective zootechnical performance. The association between cattle genotypic components linked with the season of the year proved to be the most influential factor on cattle live weight. Variables such as hair length, hematocrit, phosphatase, phosphorus, creatine phosphokinase, creatinine, protein, cortisol, calcium, and the presence of endoparasites were highlighted, demonstrating their hierarchical importance for animal selection. ML models are effective tools for establishing hierarchies of relevance in complex phenotypic multivariable, which is crucial in breeding programs for different zootechnical species and in special and specific environments like wetlands. Paraguay genotypes Brahman Brangus Nelore extinction blood clinical analyzed Initially then (ML SHAP, SHAP , (SHAP) therefore length hematocrit phosphatase phosphorus phosphokinase creatinine protein cortisol calcium highlighted selection multivariable (SHAP

Abstract Introduction. Beekeeping in Honduras is an important activity for the livestock sector, threatened by the presence of pests and diseases that affect its development and production. Objective. To determine the parasitic load of Varroa destructor and Vairimorpha (Nosema) spp. associated with hygienic behavior, productivity, and the presence of other pests in Apis mellifera hives in the municipality of Tomalá, Lempira department, Honduras. Materials and Methods. A study was carried out on 57 samples from hives in 19 apiaries to determine the infestation rate of V. destructor. The average infection rate of Vairimorpha spp. was determined using the Cantwell method. Additionally, the association between parasitic loads and productivity, hygienic behavior, and the presence of other pests was determined through a stratified analysis based on whether or not treatment against Varroa spp. was used. Associations were determined using the non-parametric Mann-Whitney U and Kruskal-Wallis tests. Results. The infestation rates for V. destructor in dispersal and larval stages were 3.48 % and 6.82 %, respectively. The use of chemical treatment showed no association with the infestation rate of V. destructor in either larvae or a dults (p≥0.05). The presence of the small hive beetle (SHB) was associated with a higher infestation of V. destructor in larvae (p<0.007) in untreated hives. The infection range of Vairimorpha spp. was classified as very light with 5×104 spores per sample. The Vairimorpha spp. load showed no association with the mentioned variables (p≥0.05). Conclusion. The results indicate that varroosis is a disease of importance in the municipality of Tomalá, Lempira department, Honduras, associated with hygienic behavior and the presence of SHB in the hives. Nosemosis showed a very light infection.

Resumen Introducción. La apicultura en Honduras es una actividad importante para el sector pecuario, amenazada por la presencia de plagas y enfermedades que afectan su desarrollo y producción. Objetivo. Determinar la carga parasitaria de Varroa destructor y Vairimorpha (Nosema) spp. asociadas al comportamiento higiénico, productividad y presencia de otras plagas en colmenas de Apis mellifera en el municipio de Tomalá, departamento de Lempira, Honduras. Materiales y métodos. Se realizó un estudio en 57 muestras de colmenas de 19 apiarios, para determinar la tasa de infestación de V. destructor. La tasa media de infección de Vairimorpha spp., se determinó mediante el método de Cantwell. Además, se determinó la asociación entre las cargas parasitarias y la productividad, comportamiento higiénico y presencia de otras plagas, mediante un análisis estratificado según el uso o no de tratamiento contra Varroa spp. La determinación de asociaciones se realizó mediante las pruebas no paramétricas U de Mann Whitney y Kruskal Wallis. Resultados. Las tasas de infestación para V. destructor en fase de dispersión y larval fueron de un 3,48 % y un 6,82 %, respectivamente, el uso de tratamiento químico no mostró asociación con la tasa de infestación de V. destructor en larvas ni en adultos (p≥0,05), la presencia del pequeño escarabajo de las colmenas (PEC), se asoció con una mayor infestación de V. destructor en larvas (p<0,007) en las colmenas sin tratamiento. El rango de infección de Vairimorpha spp. se encontró dentro de la clasificación muy ligera con 5×104 esporas por muestra, la carga de Vairimorpha spp. no mostró asociación con las variables mencionadas (p≥0,05). Conclusión. Los resultados indican que varroasis es una enfermedad de importancia en el municipio de Tomalá, departamento de Lempira, Honduras, asociada con el comportamiento higiénico y la presencia de PEC en las colmenas. Nosemosis presentó una infección muy ligera.

SUMMARY: Because horned animals are prone to conflict, they may have a higher risk of developing a brain contusion as a result of a horn strike. Repeated brain trauma can affect the hippocampus, which is involved in memory and orientation. The aim of this study was to histopathologically examine the blood-brain barrier (BBB) of the hippocampus in horned and hornless female sheep. Skulls of 4 horned and 6 hornless female sheep aged 16±4 months were obtained from the abattoir. Brains were carefully removed and preserved in 10 % formalin for 5 days. Sections were taken from bilateral hippocampus. Specimens were stained with hematoxylin-eosin (H&E) and Tunel methods, and the histopathologic structures of the BBB were examined by light microscopy. BBB damage scores evaluated. The results were analyzed by Mann-Whitney U test. The BBB damage score was found to be higher in horned female sheep. Astrocytes was more branching and BBB was more intact in hornless female sheep. This is considered that more astrocyte branches may play an important role in the protective mechanism. The difference in intracerebral defense mechanism against brain trauma in female sheep may serve as a model for investigating molecular mechanisms. The impairment of the BBB in the hippocampal region of horned female sheep may result in their memory, intelligence, and navigational abilities being lower than those of hornless female sheep. This could open the horizon for better advances in human and animal brain health.

RESUMEN: Debido a que los animales con cuernos son propensos a los conflictos, pueden tener un mayor riesgo de desarrollar una contusión cerebral como resultado de un golpe con un cuerno. Los traumatismos cerebrales reiterados pueden afectar el hipocampo relacionado con la memoria y la orientación. El objetivo de este estudio fue examinar histopatológicamente la barrera hematoencefálica (BHE) del hipocampo en ovejas con y sin cuernos. Se obtuvieron de un matadero los cráneos de 4 ovejas con cuernos y 6 sin cuernos de 16 ± 4 meses de edad. Los cerebros se extrajeron cuidadosamente y se conservaron en formalina al 10 % durante 5 días. Se tomaron secciones del hipocampo bilateral. Las muestras se tiñeron con hematoxilina-eosina (H&E) y métodos Tunel, y las estructuras histopatológicas de la BHE se examinaron mediante microscopía óptica. Se evaluaron las puntuaciones de daño de la BHE. Los resultados fueron analizados mediante la prueba U de Mann-Whitney. Se descubrió que la puntuación de daño de la BHE era mayor en las ovejas con cuernos. Los astrocitos estaban más ramificados y la BHE estaba más intacta en las ovejas sin cuernos. Se considera que más ramas de astrocitos pueden desempeñar un papel importante en el mecanismo de protección. La diferencia en el mecanismo de defensa intracerebral contra el trauma cerebral en las ovejas puede servir como modelo para investigar los mecanismos moleculares. El deterioro de la BHE en la región del hipocampo de las ovejas con cuernos puede provocar que su memoria, inteligencia y capacidades de orientación sean inferiores a las de las ovejas sin cuernos. Esto podría abrir el horizonte para mejores avances en la salud del cerebro humano y animal.

Abstract Problem: Traditionally, both the environment and other animals have been excluded from the sphere of moral consideration, even though humans’ positive relationship with them brings benefits for the health of both creatures and promotes the development of empathy and the display of prosocial behaviors (Ngai, Yu, Chau, Lee & Wong, 2021). In fact, in recent decades there have been cases of zoonoses, inappropriate use of resources, deterioration of the environment and an increase in recorded cases of animal abuse. Animal abuse, a complex and multi-determined phenomenon, frequently works as a precursor or indicator of other behaviors classified as “antisocial”, violent and non-violent. There is conflicting evidence regarding the role that empathy plays in the emission or inhibition of aggressive behaviors and how generalizable interspecies empathy is. In this respect, three phenomena of interest stand out: empathic erosion, progressive desensitization to violence and compassion fatigue. Objective: To determine whether the levels of empathy, the willingness to behave abusively against other animals, and actual animal abuse behavior are related to the context of pre-professional training, self-perceived gender, and current and/or previous ownership of other companion animals in university students who are in the final stage of their training to obtain their university degree in Psychology. Methodology: Retrospective ex post facto single case design (N=241). A self-administered questionnaire was applied that included the Interpersonal Reactivity Index, the Animal Empathy Scale, the Animal Abuse Proclivity Scale and the quantity and frequency of effective animal abuse behaviors. Contribution: the contribution of self-perceived gender to the relationship between various measures of empathy and abuse towards other animals is evidenced, and the thesis of the non-generalizability of interspecies empathy is reinforced.

Resumen Problema: tradicionalmente se ha excluido de la esfera de consideración moral tanto al ambiente como a otros animales, aun cuando una relación positiva con ellos reporta beneficios para la salud de unas y otras criaturas y promueve el desarrollo de empatía y la emisión de conductas prosociales (Ngai, Yu, Chau, Lee y Wong, 2021). De hecho, en las últimas décadas se han observado casos de zoonosis, uso inadecuado de recursos, deterioro del ambiente y un aumento de casos registrados de abuso animal. El abuso animal, fenómeno complejo y multideterminado, frecuentemente funciona como precursor o indicador de otras conductas calificadas como “antisociales”, violentas y no violentas. Respecto del rol que juega la empatía en la emisión o inhibición de conductas agresivas y de cuán generalizable es la empatía interespecie, existe evidencia contradictoria y se resaltan aquí tres fenómenos de interés: erosión empática, desensibilización progresiva a la violencia y fatiga por compasión. Objetivo: determinar si los niveles de empatía, la disposición a comportarse de manera abusiva contra otros animales y la conducta efectiva de abuso animal se relacionan con el contexto de formación preprofesional, el género autopercibido y la tenencia actual y/o previa de animales de compañía en estudiantes universitarios que cursan el tramo final de la Licenciatura en Psicología. Metodología: diseño ex post facto retrospectivo de grupo único (N = 241). Se aplicó un cuestionario autoadministrado que incluyó el Interpersonal Reactivity Index, la Animal Empathy Scale, la Animal Abuse Proclivity Scale y la cantidad y frecuencia de conductas efectivas de abuso animal. Contribución: se evidencia sobre el aporte del género autopercibido a la relación entre diversas medidas de empatía y el abuso hacia otros animales y se refuerza la tesis de la no generalizabilidad de la empatía interespecie.

The South African food system is facing severe challenges as increasing hunger, rising food costs, lack of dietary diversity, child stunting, foodborne illnesses, food waste and an obesity epidemic coupled with malnutrition are observed. In this study, we aimed to establish the application of science and technology advances in the food and beverage industry in South Africa in response to food consumption pattern changes since 1994 and how they could be used to address food security challenges. We found that food consumption shifts have been towards sugar-sweetened beverages, processed and packaged food, animal-source foods and added caloric sweeteners, and away from vegetables. These dietary shifts are concerning as they relate to public health. Most commitments to improve the nutritional status of South Africans have been limited to corporate social investment strategies and should be extended into core business strategies. Furthermore, although the South African food and beverage industry has kept pace with developments in food manufacturing practices, there has been little experimentation with non-commercial novel technologies. The expert survey revealed that indigenous African crops and food waste recovery are the two most promising emerging food sources that could be available to South Africans in the shortest time frame. South Africa has many enabling drivers to become a global leader in food technology advances. However, many barriers need to be overcome for industry, academia and government to collaborate to advance novel food science and technologies to reach commercialisation. SIGNIFICANCE: • Drivers and consequences of food consumption changes in South Africa were modelled and broad food consumption trends between 1994 and 2009/2012 were identified. • For the first time, the Access to Nutrition Index methodology was applied to South African owned food companies to identify strategies to enhance nutrition practices. Companies need to do more to deliver affordable and accessible products. • Drivers and barriers in adopting advanced food science and technology were modelled. A collective ecosystem approach with industry, academia and government mobilisation around critical areas like hunger, malnutrition and poverty could be a way to tackle the failing food system.

Abstract Background Cutaneous squamous cell carcinoma (CSCC) is one of the most common types of skin cancer worldwide. Therefore, the identification of biomarkers associated with CSCC progression could aid in the early detection of high-risk squamous cell carcinoma and the development of novel therapeutic strategies. Objective This study aimed to investigate the expression patterns of silent mating type Information Regulation 2 homolog 6 (SIRT6) in CSCC and its clinical significance. Methods The protein expression level of SIRT6 in tissues was detected by immunohistochemistry, and the correlation between SIRT6 expression and clinicopathological parameters in CSCC patients was analyzed. The relative expression of SIRT6 in CSCC cell lineage and tissue specimens was determined by western blotting and PCR. The effect of SIRT6 silencing on cell proliferation was evaluated using cell counting kit 8. Wound healing, transwell method, and flow cytometry were used to investigate the migration, invasion, and cell cycle distribution/apoptosis of CSCC cells after SIRT6 silencing, respectively. Western blot was used to detect the expression of EMT (Epithelial-Mesenchymal Transition), cycle, apoptosis, and other related proteins. Results The high expression of SIRT6 was correlated with the location of cancer tissue and Broder staging in CSCC patients. Knockdown of SIRT6 inhibited the proliferation, migration, invasion and EMT of CSCC cells, and promoted their apoptosis, with cells blocked in G1 phase. Study limitations No animal experiments were conducted to further verify the results. Conclusion Decreased expression of SIRT6 can inhibit the occurrence and development of CSCC. (CSCC worldwide Therefore highrisk risk strategies SIRT (SIRT6 significance immunohistochemistry analyzed PCR 8 healing method migration distributionapoptosis distribution apoptosis respectively EpithelialMesenchymal Epithelial Mesenchymal Transition, Transition , Transition) proteins G phase results (SIRT

ABSTRACT: The Amazonian manatee (Trichechusinunguis) is an aquatic mammal threatened with extinction. However, few studies have investigated the pathogens in this species, which may affect both animal and human health. This study aimed to evaluate the frequency, distribution, and patterns of antimicrobial susceptibility of Staphylococcus spp. and Escherichiacoli colonizing the nasal and rectal cavities of Amazonian manatees kept in captivity at the National Institute for Amazonian Research (INPA) in the state of Amazonas, Brazil. Rectal and nasal swabs from 44 manatees of different ages were used in this study. The genus Staphylococcus was isolated from the nasal swabs of 32 (72.7%) animals, with two individuals harboring more than one species of Staphylococcus. S. sciuri was the most commonly isolated species. Resistance to penicillin was observed in 13 (40.6%) isolates, more frequent than the other antimicrobials tested (P = 0.01). E. coli was isolated from the rectal swabs of all animals, with phylogroup B1 being the most frequent among the strains obtained (P = 0.0008). Four isolates (6.8%) were positive for virulence factors, three of which were classified as enterotoxigenicE. coli (ETEC) and one as enteropathogenicE. coli (EPEC). To our knowledge, this is the first study to evaluate Staphylococcus spp. and E. coli in Amazonian manatee samples. This study revealed nasal colonization by Staphylococcus spp., mainly S. sciuri, and diarrheagenicE. coli isolates, including antimicrobial-resistant strains. ABSTRACT Trichechusinunguis (Trichechusinunguis extinction However health frequency distribution spp INPA (INPA Amazonas Brazil 4 3 72.7% 727 72 7 (72.7% animals S 1 40.6% 406 40 6 (40.6% P 0.01. 001 0.01 . 0 01 0.01) E B 0.0008. 00008 0.0008 0008 0.0008) 6.8% 68 8 (6.8% factors enterotoxigenicE ETEC (ETEC enteropathogenicE EPEC. EPEC (EPEC) knowledge samples diarrheagenicE antimicrobialresistant resistant 72.7 (72.7 40.6 (40.6 00 0.0 0000 0.000 000 6.8 (6.8 (EPEC 72. (72. 40. (40. 0. 0.00 6. (6. (72 (40 (6 (7 (4 (

RESUMO: O peixe-boi amazônico (Trichechus inunguis) é um mamífero aquático ameaçado de extinção. Apesar disso, poucos estudos investigaram patógenos nessa espécie que podem impactar a saúde animal e humana. O objetivo deste estudo foi avaliar a frequência, distribuição e padrões de suscetibilidade antimicrobiana de Staphylococcus sp. e Escherichia coli pertencentes à microbiota nasal e retal de peixes-bois da Amazônia mantidos em cativeiro no Instituto Nacional de Pesquisas da Amazônia (INPA) (Amazonas, Brasil). Foram utilizados suabes retais e nasais de 44 peixes-boi de diferentes idades. O gênero Staphylococcus foi isolado do swab nasal de 32 (72,7%) animais, sendo que dois indivíduos apresentaram mais de uma espécie de Staphylococcus. S. sciuri foi a espécie mais comumente isolada. A resistência à penicilina foi observada em 13 (40,6%) isolados, sendo mais frequente do que os outros antimicrobianos testados (P = 0,01). Escherichia coli foi isolada dos suabes retais de todos os animais, sendo o filogrupo B1 o mais frequente entre as cepas obtidas (P = 0,0008). Quatro isolados (6,8%) foram positivos para fatores de virulência, três dos quais foram classificados como E. coli enterotoxigênica (ETEC) e um isolado como E. coli enteropatogênica (EPEC). Este é o primeiro estudo a avaliar Staphylococcus spp. e E. coli de amostras de peixe-boi da Amazônia. O estudo revelou colonização nasal por Staphylococcus sp., principalmente S. sciuri, e por isolados diarreiogênicos de E. coli, incluindo cepas resistentes a antimicrobianos. RESUMO peixeboi peixe boi Trichechus inunguis extinção disso humana frequência sp peixesbois peixes bois INPA (INPA Amazonas, Amazonas (Amazonas Brasil. Brasil . Brasil) 4 peixesboi idades 3 72,7% 727 72 7 (72,7% animais S 1 40,6% 406 40 6 (40,6% P 0,01. 001 0,01 0 01 0,01) B 0,0008. 00008 0,0008 0008 0,0008) 6,8% 68 8 (6,8% virulência E ETEC (ETEC EPEC. EPEC (EPEC) spp 72,7 (72,7 40,6 (40,6 00 0,0 0000 0,000 000 6,8 (6,8 (EPEC 72, (72, 40, (40, 0, 0,00 6, (6, (72 (40 (6 (7 (4 (

Abstract Objective: To analyze the arguments and sources about veganism/vegetarianism in 2022 in the Mexican press, with the objective of how these movements are portrayed. Methodology: Applying content analysis, the arguments were classified by themes in an inductive way, identifying the sources. Results: Health, sustainability, and animal wellbeing are the issues most linked to these movements, portrayed in a positive light. Veganism had more prominence, and the own newsroom of the newspapers was the most cited source, followed by celebrities, scientific sources, and activists. Limitations: The analysis is limited to the Mexican press. Conclusions: A Latin American perspective is brought to studies on veganism/vegetarianism in the media. The coverage of veganism/vegetarianism by the Mexican press in 2022 was predominantly positive, so the pattern observed in previous press analysis in Anglo-Saxon countries is not repeated. Veganism seems to be more popular than vegetarianism.

Resumen Objetivo: Analizar los argumentos y fuentes sobre el veganismo/vegetarianismo en 2022 presentes en la prensa mexicana, con el objetivo de comprobar cómo se retratan estos movimientos. Metodología: Aplicando el análisis de contenido, los argumentos se clasificaron por temas de forma inductiva, identificando las fuentes. Resultados: Salud, sostenibilidad y bienestar animal son los temas más vinculados a estos movimientos, retratados de forma positiva. El veganismo tuvo más protagonismo y la redacción de los periódicos fue la fuente principal, seguida de celebridades, fuentes científicas y activistas. Limitaciones: El análisis se limita a la prensa mexicana. Conclusiones: Se aporta una perspectiva latinoamericana a estudios sobre veganismo/vegetarianismo en los medios. La cobertura del veganismo/vegetarianismo por parte de la prensa mexicana en 2022 fue predominantemente positiva, por lo que no se repite el patrón observado en análisis anteriores de prensa de países anglosajones. El veganismo parece estar más en auge que el vegetarianismo.

Resumen: El estudio de la microbiota ha adquirido un nuevo enfoque de interés, ya que interviene en diversos procesos fisiológicos involucrados en el desarrollo y rendimiento de los animales domésticos. Participa en el eje-microbiota-intestino-cerebro, con procesos que rigen el sistema nervioso hacia el intestino y viceversa. El objetivo de este trabajo fue identificar la importancia de la microbiota gastrointestinal de pollos de engorda y gallinas de postura y su relación con procesos fisiológicos que afecten el desarrollo, rendimiento, comportamiento y salud. En el tracto gastrointestinal de estas aves se han identificado alrededor de 17 filos de bacterias, con microorganismos predominantes según el sitio anatómico dentro del tracto y por ende con diferente función, por ejemplo, buche: Lactobacillus, Enterobacteriaceae; proventrículo: Clostridiaceae, Enterococcus; intestino: Escherichia, Streptococcus. Distintos factores intervienen en la colonización y desarrollo de la microbiota, desde el programa de alimentación y manejo, tipo de cama y densidad animal, entre otros. Existen microorganismos potencialmente patógenos que impactan negativamente en la microbiota y puedan llegar al consumidor, como Campylobacter jejuni, Salmonella enteritidis y Escherichia coli, de ahí su importancia al momento de trazar las directrices en las producciones pecuarias.

Abstract: There is an increasing interest in microbiota studies due to their relevance in physiological processes such as animal development and productivity. In addition, it its involved in the microbiota-intestinebrain axis that regulates the nervous system to the intestine and vice versa. This paper is aimed at identifying the importance of the broilers and laying hens’ gastrointestinal microbiota and its relationship with diverse physiological processes that intervene in the development, productivity, behavior, and health. There are 17 filos of bacteria within the poultry gastrointestinal tract, which are site dependent and have specific functions (Example; crop: Lactobacillus, Enterobacteriaceae; proventriculus: Clostridiaceae, Enterococcus; intestine: Escherichia, Streptococcus). In addition, different factors affect the development of microbiota such as the feeding program, handling, kind of substrate, stocking density, among others. There are several potentially pathogenic microorganisms that impact microbiota negatively and may reach the final consumer such as Campylobacter jejuni, Salmonella enteritidis and Escherichia coli. Therefore, it is important to continue supporting poultry microbiota research and areas of opportunity to improve poultry production.

Resumen: La introducción de la parafiscalidad para fortalecer los programas de salud animal en Colombia trajo profundos cambios en el gobierno del sector pecuario del país, al pasar de un modelo público a uno de cooperación público-privada. La investigación analiza los mecanismos de gobernanza que a partir de estos cambios se constituyeron en la ganadería, avicultura y porcicultura, con el fin de revisar la incidencia de los ajustes en los avances del control sanitario. Para ello, se realizó un estudio de casos múltiples de los fondos de fomento pecuario de los tres subsectores, en el que se evidencia que la parafiscalidad y el modelo de asociación público-privada han permitido la consolidación formal y en la práctica para el control de enfermedades en el país.

Abstract: The introduction of quasi-tax to strengthen animal health programs in Colombia brought profound changes in the government of the country’s livestock sector by moving from a public model to one of public-private cooperation. The research analyzes the governance mechanisms that were established in livestock, poultry, and pig farming, in order to review the incidence of adjustments in the progress of health control. For this purpose, a multiple case study of the livestock promotion funds of the three subsectors was carried out, which showed that quasi-tax and the public-partnership model have allowed formal and practical consolidation for disease control in the country.

Abstract The management of silvopastoral systems associated with carob trees (Neltuma spp.) as a tree component constitutes a forestry potential for the Chaco ecoregion, in addition to benefiting animal well-being by providing shade, an alternative source of forage, protection against wind and the effects adverse effects of temperature extremes, and mitigate the effects of climate change by storing carbon in both biomass and soil, thus constituting an integrated sustainable production system for the Paraguayan Chaco. The objective was to evaluate the management of the regeneration of carob trees integrated into silvopastoral systems of the Paraguayan Central Chaco. For this, the survival of carob trees subjected to silvicultural treatments in silvopastoral systems was determined, the increase in diameter of carob trees subjected to silvicultural treatments in silvopastoral systems was evaluated, determining the basal area, volume, biomass, accumulated carbon and carbon dioxide, equivalent carbon in the studied systems, study the phenology and health status of the inventoried tree individuals, characterize the herbaceous animal component and determine the physical and chemical properties of the soils of the paddocks under study. The results obtained refer to a high survival of carob trees in silvopastoral systems, with the averages of the dasometric variables in terms of basal area being 11.99 m2/ha, stem volume being 10.93 m3/ha and total volume being 75. 48 m3/ha. At the time of the inventory, it was recorded that 74% of the individuals were in flowering and 68% were sick. The herbaceous component was made up of Gatton panic (Panicum maximun) with an animal load of 0.8 AU and only one of the three paddocks had compacted soils. The accumulated carbon dioxide content was similar in the three pastures. It is concluded that the silvopastoral systems associated with Neltuma spp. As a tree component, they constitute a forest potential for sustainable production in the Chaco ecoregion.

Resumen El manejo de los sistemas silvopastoriles asociados con los algarrobos (Neltuma spp.) como componente arbóreo constituyen un potencial forestal para la ecorregión chaqueña, además de beneficiar al bienestar animal por suministro de sombra, fuente alternativa de forraje, protección contra el viento y los efectos adversos de los extremos de temperatura, y mitigar los efectos del cambio climático por el almacenamiento de carbono tanto en la biomasa como el suelo, por lo que constituye un sistema integrado de producción sustentable para el Chaco paraguayo. El objetivo fue evaluar la regeneración natural de algarrobos (Neltuma spp.) integrados a sistemas silvopastoriles del Chaco Central Paraguayo como respuesta manejo silvicultural. Para ello, se determinó la sobrevivencia de algarrobos sometidos a tratamientos silviculturales en sistemas silvopastoriles, se evaluó el incremento en diámetro de algarrobos, el área basal, el volumen, la biomasa, el carbono acumulado y dióxido de carbono equivalente, la fenología y el estado sanitario de los individuos arbóreos inventariados, el componente herbáceo y animal, y las propiedades físicas y químicas de los suelos. Los resultados obtenidos refieren una sobrevivencia alta de algarrobos en los sistemas silvopastoriles, siendo los promedios de las variables dasométricas en cuanto a área basal de 11,99 m2/ha, volumen de fuste de 10,93 m3/ha y del volumen total 75,48 m3/ha. Al momento del inventario se registró que 74% de los individuos se encontraban en floración y 68% enfermos. El componente herbáceo estuvo integrado por Gatton panic (Panicum maximun) con una carga animal de 0,8 UA y solo uno de los tres potreros presentó suelos compactados.El contenido de anhidrido carbónico acumulado fue similar en los tres potreros. Se concluye que los sistemas silvopastoriles asociados con Neltuma spp. como componente arbóreo constituyen un potencial forestal para producción sustentable de la ecorregión chaqueña.

Abstract: In vitro propagation protocols for Theobroma cacao have focused on the multiplication of genotypes with the use of 2,4-diclorofenoxiacetic acid (2,4-D) and thidiazuron (TDZ) in the initial phase of the process. There are no reports of the use of indole-3-acetic acid (AIA) as a source of auxin. Due to risks for human health and the environment, international bans have been placed on the use of 2,4-D. The objective of this study was to evaluate the capacity of AIA in combination with TDZ as inducers of embryogenic usfrom staminodes and petals of the Creole and Trinitarian genotypes of T. cacao. The results showed that the combination of AIA 1.0 mg L-1 and TDZ0.005 mg L-1 induced callus in 100 % of explants; the rest of the combinations of AIA and TDZ induced callus in 50 % and 80 % of explants. In conclusion, the use of AIA is useful for the formation of friable callus in in vitro cultures of T. cacao.

ABSTRACT This article analyzes Calvo’s work based on his attempt to narrate the disease(s) outside the topics of biomedical discourse. His main objective is to explain how the main features of the jungle novel are subverted by making a vindication of the actions and rhetoric of the Amazonian healers. In this way, our analysis focuses on the healing practices that revolve around the ingestion of ayahuasca and how they are incorporated into Calvo's fiction not only as a dissemination of the knowledge of the vegetalistas but also resulting in the exposition of the core of the disease: the disharmonization of the subject and its breaking with the ties that, otherwise, would manage to grasp its macroscopic kinship with the animal and the plant. In conclusion, the record of the intimate that Calvo’s fiction puts into operation introduces not only another way of approaching the topic of illness but also transforming the travel diary through the jungle into a diary of the illness capable of stressing the sacred techniques of sorcerers and the imaginary of health understood from a secular perspective. Calvos Calvo s diseases disease disease(s discourse healers otherwise plant conclusion perspective

RESUMEN Este artículo analiza la obra de Calvo a partir de su intento de narrar la(s) enfermedad(es) por fuera de los tópicos del discurso biomédico. Su objetivo principal es dar cuenta de cómo se subvierten los rasgos principales de la novela de la selva al realizar una reivindicación del accionar y de la retórica de los curanderos amazónicos. De esta forma, nuestro análisis se centra en las prácticas curativas que orbitan alrededor de la ingesta de ayahuasca y en cómo son incorporadas a la ficción de Calvo no solamente como divulgación de los saberes de los vegetalistas, sino también dando por resultado la exposición del meollo de la enfermedad: la desarmonización del sujeto y su ruptura con los lazos que, de lo contrario, lograrían asir su parentesco macroscópico con lo animal y lo vegetal. En conclusión, el registro de lo íntimo que pone en funcionamiento la ficción de Calvo introduce otra forma de abordar el tópico de la enfermedad y, asimismo, transforma el diario de viaje por la selva en un diario de la enfermedad capaz de tensionar las técnicas sagradas de los brujos y el imaginario de lo saludable entendido desde una óptica secular. s la(s enfermedades enfermedad(es biomédico amazónicos vegetalistas contrario vegetal conclusión asimismo secular

Abstract Introduction: Even though only a few species are considered to be dangerous, pests or vectors, the majority of invertebrates produce a feeling of aversion in humans. This has contributed to the delay in the development of ethical considerations as regards this group in contrast with vertebrates, with the exception of cephalopods. Objective: In the present study, we provide an overview of the current situation on animal ethics and welfare in order to contribute to the development of a framework for ensuring invertebrate welfare. Methods: Today, animal welfare is multidisciplinary in nature to a very high degree as it includes ethology, physiology, pathology, biochemistry, genetics, immunology, nutrition, cognitive-neural, veterinary medicine, and ethics. Animal welfare is a complex concept, difficult to achieve successfully from one perspective. Results: As a consequence, we propose to include the five domains (nutrition, environment, health, behaviour and mental state) along with the three conceptions (basic health and functioning, affective state and natural living), as well as the 5R Principle (Replace, Reduction, Refinement, Respect and Responsibility) in seeking to achieve a comprehensive welfare state. Conclusions: We consider that in both research and animal production, the individual and collective ethical concerns coexist and, in fact, the main moral concern to account for is the collective one and that, within that collective view, the individual moral concern should be applied with responsibility and respect for the individual. Finally, we propose a practical example of invertebrate welfare production in sea urchin aquaculture with the aim of including animal production of invertebrates in this important discussion.

Resumen Introducción: Aunque sólo unas pocas especies son consideradas peligrosas, plagas o vectores, la mayoría de los invertebrados producen un sentimiento de aversión en el ser humano. Esto ha contribuido al retraso en el desarrollo de consideraciones éticas respecto a este grupo en comparación con los vertebrados, a excepción de los cefalópodos. Objetivo: En el presente trabajo, proporcionamos una visión general de la situación actual en materia de ética y bienestar animal con el fin de contribuir al desarrollo de un marco para garantizar el bienestar de los invertebrados. Métodos: Hoy en día, el bienestar animal es de naturaleza multidisciplinaria en un grado muy alto, ya que incluye etología, fisiología, patología, bioquímica, genética, inmunología, nutrición, cognitivo-neural, medicina veterinaria y ética. El bienestar animal es un concepto complejo, difícil de lograr con éxito desde una sola perspectiva. Resultados: Como consecuencia, proponemos incluir los cinco dominios (nutrición, ambiente, salud, comportamiento y estado mental) junto con las tres concepciones (Salud básica y funcionamiento, estado afectivo y vida natural), así como el Principio 5R (Reemplazar, Reducir, Refinar, Respetar y Responsabilidad) en la búsqueda de alcanzar un estado de bienestar integral. Conclusiones: Consideramos que tanto en la investigación como en la producción animal coexisten las preocupaciones éticas individuales y colectivas y, de hecho, la principal preocupación moral a dar cuenta es la colectiva y que, dentro de esa visión colectiva, se debe aplicar la preocupación moral individual. con responsabilidad y respeto por la persona. Finalmente, proponemos un ejemplo práctico de producción de bienestar de invertebrados en la acuicultura de erizos de mar con el objetivo de incluir la producción animal de invertebrados en esta importante discusión.

ABSTRACT The aim of this study was to describe the ownership of pets and knowledge of zoonoses among residents in the district of Bambamarca - Cajamarca, Peru, in 2022. In total, 394 surveys were carried out, 30.5% in the urban area (Bambamarca city) and 69.5% in the rural area (Llaucán, San Juan de Lacamaca, Chala and El Tuco). Of those surveyed, 54.8% were female, 20.8% had completed secondary school and the average age was 39.9 years. Besides, 58.4 and 35.5% of interviewees claimed to own dogs and cats, respectively. The person: dog ratio was 4:1 and person: cat was 6.7: 1; 45.2% of dog owners raised them as pets and 45% of cat owners raised them as rodent pest controllers; 67.9% of the dogs were males and 50.5% of the cats were females. The rate of sterilized animals was less than 4% in both species. In the case of dogs, 54.1% walked without restriction and 31.7% of cats were classified as «outdoor»; 50.3% of dog owners and 69.5% of cat owners mentioned performing internal deworming, and 45 and 61.5%, respectively, performed external deworming on a regular basis (at least every 3 months). Moreover, 58% of dogs and 12.8% of cats were reported to have been vaccinated against rabies in the last year. Ignorance of the meaning of the term zoonosis reached 95.4% of those surveyed; however, 90.4% said they knew that animals could transmit diseases to people, the most recognized being rabies (69.8%). It is concluded that it is necessary to develop health education campaigns so that pet owners develop responsible ownership and thus avoid negative impacts on public health and animal welfare.

RESUMEN El estudio tuvo como objetivo describir la tenencia de animales de compañía y los conocimientos de zoonosis entre pobladores en el distrito de Bambamarca - Cajamarca, Perú, en 2022. Se realizaron 394 encuestas, siendo 30.5% en la zona urbana (Bambamarca ciudad) y 69.5% en la zona rural (Llaucán, San Juan de Lacamaca, Chala y El Tuco). De los encuestados, 54.8% fueron mujeres, 20.8% contaban con secundaria completa y la edad promedio fue de 39.9 años. El 58.4 y 35.5% de entrevistados aseguraron poseer perros y gatos, respectivamente. La relación persona: perro fue 4:1 y persona: gato de 6.7: 1. El 45.2% de poseedores de canes lo criaban como mascota y el 45% de poseedores de gatos los criaban como controladores de plaga de roedores. El 67.9% de los perros fueron machos y 50.5% de los gatos fueron hembras. La tasa de animales esterilizados fue menor al 4% en ambas especies. En caso de los perros, 54.1% paseaban sin restricción y 31.7% de los gatos se clasificaron como «outdoor». El 50.3% de tenedores de perros y 69.5% de tenedores de gatos mencionaron realizar desparasitaciones internas, y 45 y 61.5%, respectivamente, desparasitaban externamente de manera regular (al menos cada 3 meses). El 58% de perros y el 12.8% de gatos fueron reportados como vacunados contra la rabia el último año. El desconocimiento del significado del término zoonosis alcanzó al 95.4% de los encuestados; sin embargo, el 90.4% afirmó conocer que los animales podrían transmitir enfermedades a las personas, siendo la más reconocida la rabia (69.8%). Se concluye que es necesario desarrollar campañas de educación sanitaria a fin de que los propietarios de animales de compañía desarrollen una tenencia responsable y evitar de esta forma impactos negativos sobre la salud pública y el bienestar animal.