Abstract Background Cutaneous squamous cell carcinoma (CSCC) is one of the most common types of skin cancer worldwide. Therefore, the identification of biomarkers associated with CSCC progression could aid in the early detection of high-risk squamous cell carcinoma and the development of novel therapeutic strategies. Objective This study aimed to investigate the expression patterns of silent mating type Information Regulation 2 homolog 6 (SIRT6) in CSCC and its clinical significance. Methods The protein expression level of SIRT6 in tissues was detected by immunohistochemistry, and the correlation between SIRT6 expression and clinicopathological parameters in CSCC patients was analyzed. The relative expression of SIRT6 in CSCC cell lineage and tissue specimens was determined by western blotting and PCR. The effect of SIRT6 silencing on cell proliferation was evaluated using cell counting kit 8. Wound healing, transwell method, and flow cytometry were used to investigate the migration, invasion, and cell cycle distribution/apoptosis of CSCC cells after SIRT6 silencing, respectively. Western blot was used to detect the expression of EMT (Epithelial-Mesenchymal Transition), cycle, apoptosis, and other related proteins. Results The high expression of SIRT6 was correlated with the location of cancer tissue and Broder staging in CSCC patients. Knockdown of SIRT6 inhibited the proliferation, migration, invasion and EMT of CSCC cells, and promoted their apoptosis, with cells blocked in G1 phase. Study limitations No animal experiments were conducted to further verify the results. Conclusion Decreased expression of SIRT6 can inhibit the occurrence and development of CSCC. (CSCC worldwide Therefore highrisk risk strategies SIRT (SIRT6 significance immunohistochemistry analyzed PCR 8 healing method migration distributionapoptosis distribution apoptosis respectively EpithelialMesenchymal Epithelial Mesenchymal Transition, Transition , Transition) proteins G phase results (SIRT

ABSTRACT: The Amazonian manatee (Trichechusinunguis) is an aquatic mammal threatened with extinction. However, few studies have investigated the pathogens in this species, which may affect both animal and human health. This study aimed to evaluate the frequency, distribution, and patterns of antimicrobial susceptibility of Staphylococcus spp. and Escherichiacoli colonizing the nasal and rectal cavities of Amazonian manatees kept in captivity at the National Institute for Amazonian Research (INPA) in the state of Amazonas, Brazil. Rectal and nasal swabs from 44 manatees of different ages were used in this study. The genus Staphylococcus was isolated from the nasal swabs of 32 (72.7%) animals, with two individuals harboring more than one species of Staphylococcus. S. sciuri was the most commonly isolated species. Resistance to penicillin was observed in 13 (40.6%) isolates, more frequent than the other antimicrobials tested (P = 0.01). E. coli was isolated from the rectal swabs of all animals, with phylogroup B1 being the most frequent among the strains obtained (P = 0.0008). Four isolates (6.8%) were positive for virulence factors, three of which were classified as enterotoxigenicE. coli (ETEC) and one as enteropathogenicE. coli (EPEC). To our knowledge, this is the first study to evaluate Staphylococcus spp. and E. coli in Amazonian manatee samples. This study revealed nasal colonization by Staphylococcus spp., mainly S. sciuri, and diarrheagenicE. coli isolates, including antimicrobial-resistant strains. ABSTRACT Trichechusinunguis (Trichechusinunguis extinction However health frequency distribution spp INPA (INPA Amazonas Brazil 4 3 72.7% 727 72 7 (72.7% animals S 1 40.6% 406 40 6 (40.6% P 0.01. 001 0.01 . 0 01 0.01) E B 0.0008. 00008 0.0008 0008 0.0008) 6.8% 68 8 (6.8% factors enterotoxigenicE ETEC (ETEC enteropathogenicE EPEC. EPEC (EPEC) knowledge samples diarrheagenicE antimicrobialresistant resistant 72.7 (72.7 40.6 (40.6 00 0.0 0000 0.000 000 6.8 (6.8 (EPEC 72. (72. 40. (40. 0. 0.00 6. (6. (72 (40 (6 (7 (4 (

RESUMO: O peixe-boi amazônico (Trichechus inunguis) é um mamífero aquático ameaçado de extinção. Apesar disso, poucos estudos investigaram patógenos nessa espécie que podem impactar a saúde animal e humana. O objetivo deste estudo foi avaliar a frequência, distribuição e padrões de suscetibilidade antimicrobiana de Staphylococcus sp. e Escherichia coli pertencentes à microbiota nasal e retal de peixes-bois da Amazônia mantidos em cativeiro no Instituto Nacional de Pesquisas da Amazônia (INPA) (Amazonas, Brasil). Foram utilizados suabes retais e nasais de 44 peixes-boi de diferentes idades. O gênero Staphylococcus foi isolado do swab nasal de 32 (72,7%) animais, sendo que dois indivíduos apresentaram mais de uma espécie de Staphylococcus. S. sciuri foi a espécie mais comumente isolada. A resistência à penicilina foi observada em 13 (40,6%) isolados, sendo mais frequente do que os outros antimicrobianos testados (P = 0,01). Escherichia coli foi isolada dos suabes retais de todos os animais, sendo o filogrupo B1 o mais frequente entre as cepas obtidas (P = 0,0008). Quatro isolados (6,8%) foram positivos para fatores de virulência, três dos quais foram classificados como E. coli enterotoxigênica (ETEC) e um isolado como E. coli enteropatogênica (EPEC). Este é o primeiro estudo a avaliar Staphylococcus spp. e E. coli de amostras de peixe-boi da Amazônia. O estudo revelou colonização nasal por Staphylococcus sp., principalmente S. sciuri, e por isolados diarreiogênicos de E. coli, incluindo cepas resistentes a antimicrobianos. RESUMO peixeboi peixe boi Trichechus inunguis extinção disso humana frequência sp peixesbois peixes bois INPA (INPA Amazonas, Amazonas (Amazonas Brasil. Brasil . Brasil) 4 peixesboi idades 3 72,7% 727 72 7 (72,7% animais S 1 40,6% 406 40 6 (40,6% P 0,01. 001 0,01 0 01 0,01) B 0,0008. 00008 0,0008 0008 0,0008) 6,8% 68 8 (6,8% virulência E ETEC (ETEC EPEC. EPEC (EPEC) spp 72,7 (72,7 40,6 (40,6 00 0,0 0000 0,000 000 6,8 (6,8 (EPEC 72, (72, 40, (40, 0, 0,00 6, (6, (72 (40 (6 (7 (4 (

Abstract Common duckweeds are flowering plants of the family Araceae, comprising the smallest angiosperms of the plant kingdom, a species of aquatic algae of universal distribution, found on the surface of freshwater bodies, mainly in puddles, swamps, lakes, and calm rivers. Recently, different research has been carried out on its potential and usefulness. Due to its nutritional composition, protein contribution, high fiber content and low fat and carbohydrate content, it would be an adequate input to generate products of high nutritional value, characteristics that make it interesting compared to other species. It is used as a complement to commercial diets in a wide variety of animals such as birds, ruminants, non-ruminants, crustaceans, and fish, reducing feed costs by up to 50 %. Likewise, used in remediation processes of a wide range of chemical contaminants with a high elimination rate, they can absorb some dissolved substances and provide oxygen through photosynthesis. It has been indicated that they are low cost of construction, maintenance, easy to operate, have a wide tolerance to growing conditions, are generally easy to harvest, and do not compete with farmland. In the environmental field, it is important to find alternative and innovative raw materials, even without the need to use growth media or fertilizers, however, their acceptance as a food source needs extensive research regarding their nutritional value, large-scale yield, economic market supply and analysis of antinutritive components for human food.

Resumen Las lentejas de agua son plantas con flores de la familia Aráceas, comprenden las angiospermas más pequeñas del reino vegetal, una especie de algas acuáticas de distribución universal, se encuentran en la superficie de los cuerpos de agua dulce principalmente en charcos, ciénagas, lagos y ríos calmados. Recientemente, se han llevado a cabo diferentes investigaciones sobre su potencial y utilidad. Por su composición nutricional, aporte de proteína, alto contenido de fibra y bajo contenido de grasas y carbohidratos, resultaría ser un insumo adecuado para generar productos de alto valor nutricional, características que la hacen interesante frente a otras especies. Se emplea como complemento a dietas comerciales en una gran variedad animales como aves, rumiantes, no rumiantes, crustáceos y peces, reduciendo hasta un 50 % los costos por alimentación. Así mismo, usada en procesos de remediación de una amplia gama de contaminantes químicos con alta tasa de eliminación, pueden absorber algunas sustancias disueltas y brindar oxígeno mediante la fotosíntesis. Se ha indicado bajo costo de construcción, mantenimiento, fáciles de operar, poseen amplia tolerancia a condiciones de crecimiento, facilidad general de cosecha y no compiten con las tierras de cultivo. En el ámbito ambiental es importante encontrar materias primas alternativas e innovadoras, incluso sin la necesidad de utilizar medios de crecimiento o fertilizantes, sin embargo, su aceptación como fuente de alimento necesita investigaciones exhaustivas con respecto a su valor nutritivo, rendimiento a gran escala, suministro de mercado económico y análisis de componentes antinutritivos para la alimentación humana.

Abstract In order to establish the incidence and distribution of termite populations on native grassland (Paspalum notatum L.) production, cattle farms and their surroundings were examined in Itacurubi del Rosario, department of San Pedro, Paraguay; using the GPS nest location method (Garmin Map 76 CSx), drones, capture of aerial photographs and then the evaluation of the incidence and distribution of termite nests per hectare. Maps of incidence and distribution of nests were made for a surface of seven hectares. From the data generated, a map with the distribution of active termite nests was prepared using the QGIS software version 3.18. The formation of termite mounds represented an average area of 12 m² and an intensity of 13.5% per hectare, distributed in an average of 96.2 nests per hectare, totaling some 8,085 termite nests within the study area for a total area of 7 ha. Examples of species were represented by the family: Termitidae (Cornitermes cumulans, Nasutitermes nigriceps and Termes sp.) of wide distribution in the study area. The presence of termite mounds negatively affects the useful productive area due to the occupied area and a significant loss of pasture per day to feed the animals in each season of the year.

Resumen Con el fin de evaluar la incidencia y distribución de las poblaciones de termitas sobre la producción del pastizal nativo (Paspalum notatumL.), se muestrearon fincas ganaderas y su entorno en Itacurubi del Rosario - Departamento de San Pedro - Paraguay. Los termiteros fueron localizados utilizando un GPS de la marca Garmin Map 76 CSx y drones equipados con cámaras fotográficas para las capturas de fotografías aéreas. Se realizaron mapas de incidencia y distribución de termiteros para la superficie de uno y 7,4 hectáreas. A partir de los datos generados, se elaboró un mapa con la distribución de los termiteros activos mediante el software QGIS versión 3.18. La formación de los termiteros representó una superficie promedio de 12 m² en promedio y una intensidad de 13,5% por hectárea, distribuidos en un promedio de 96,2 nidos por hectárea, totalizando unos 711,88 termiteros dentro del área de estudio para una superficie total de 7,4 ha. La familia Termitidae estuvo representadas por las siguientes especies: (Cornitermes cumulans,Nasutitermes nigricepsyTermessp.) de amplia distribución en el área de estudio. La cantidad de termiteros encontrados afecta la superficie útil productiva del pastizal, así como a la disponibilidad de pastos para la alimentación animal en las distintas épocas del año.

Abstract To determine the species composition, relative abundance and seasonal variation of different mosquitoes Genera (Aedes, Anopheles, Armigeres, Culex, and Culiseta) in different habitats the present research work was carried out in Entomology Research Laboratory of The University of Peshawar. Sampling performed from variety of permanent and temporary breeding habitats was carried out on monthly basis from targeted breeding sites for two consecutive years through dipping method. Species diversity in the survey sites was noted. Collection from these seventeen various types of potential larval habitats, yielded a total of 42,430 immature constituting 41,556 larvae and 874 pupae. Among these only 19,651 adult mosquitoes emerged comprising 11,512 female and 8,139 male mosquitoes. 78% (n= 15333) of mosquito larvae were from permanent and 22% (n=4318) were from temporary breeding sites. This study showed that Peshawar valley harbours 15 species from the genera Aedes, Anopheles, Armigeres, Culex and Culiseta. When the density of each species was examined, Culex quinquifasciatus was found to be dominant (79%) and constant in distribution. Among the temporary habitats Aedes albopictus was found as the most prevalent species particularly from tree holes and water cisterns. The highest intensity of mosquitoes was in June (2243 emerged adults) and November (2667 emerged adults) while the lowest was in January (203 emerged adults). A perfect positive correlation (r = +0.8) was found between temperature and population of mosquitoes (df 10 and α 0.05). The species diversity index for mosquitoes remained between 0.12 and 1.76. The Margalef’s richness components was noticeably low for bamboo traps (0.2) and fairly high for rice fields, Percolating water and Animal tracks (1.3) which shows the abundance of mosquito species in these habitats. Similarly Pielou’s Evenness was highest for bamboo traps (E=1) showing species uniform distribution. Animal tracks were presumed not only the diverse habitat rather also possessed high value for species richness and species evenness. Temperature, rainfall, humidity and other related attributes responsible for species variation and abundance need to be analysed further to pave way for controlling vector species in their oviposition targeted sites. composition (Aedes Anopheles Armigeres Culiseta method noted 42430 42 430 42,43 41556 41 556 41,55 87 pupae 19651 19 651 19,65 11512 11 512 11,51 8139 8 139 8,13 78 n= n (n 15333 22 n=4318 n4318 4318 (n=4318 1 examined 79% 79 (79% distribution cisterns 2243 (224 adults 2667 (266 203 (20 adults. . r +0.8 08 0 df 0.05. 005 0.05 05 0.05) 012 12 0.1 176 76 1.76 Margalefs Margalef s 0.2 02 2 (0.2 fields 1.3 13 3 (1.3 Pielous Pielou E=1 E1 E (E=1 evenness Temperature rainfall 4243 4 43 42,4 4155 55 41,5 1965 65 19,6 1151 51 11,5 813 8,1 7 1533 n=431 n431 431 (n=431 (79 224 (22 266 (26 20 (2 +0. 00 0.0 01 0. 17 1.7 (0. 1. (1. E= (E= 424 42, 415 5 41, 196 6 19, 115 11, 81 8, 153 n=43 n43 (n=43 (7 26 ( +0 (0 (1 (E n=4 n4 (n=4 +

Resumo Para determinar a composição de espécies, abundância relativa e variação sazonal de diferentes gêneros de mosquitos (Aedes, Anopheles, Armigeres, Culex e Culiseta) em diferentes habitats, o presente trabalho foi realizado no Laboratório de Pesquisa em Entomologia da Universidade de Peshawar. A amostragem coletada a partir de uma variedade de habitats de reprodução permanentes e temporários foi realizada mensalmente a partir de locais de reprodução alvo por 2 anos consecutivos através do método de imersão. A diversidade de espécies nos locais de pesquisa foi anotada. A coleta desses 17 tipos diferentes de habitats larvais potenciais rendeu um total de 42.430 imaturos, constituindo 41.556 larvas e 874 pupas. Destes, emergiram apenas 19.651 mosquitos adultos, sendo 11.512 fêmeas e 8.139 machos. 78% (n = 15333) das larvas do mosquito eram de criadouros permanentes e 22% (n = 4318) de criadouros temporários. Este estudo mostrou que o vale de Peshawar abriga 15 espécies dos gêneros Aedes, Anopheles, Armigeres, Culex e Culiseta. Quando a densidade de cada espécie foi examinada, Culex quinquifasciatus foi considerado dominante (79%) e constante na distribuição. Entre os habitats temporários, o Aedes albopictus foi encontrado como a espécie mais prevalente, principalmente em ocos de árvores e cisternas de água. A maior intensidade de mosquitos foi em junho (2.243 adultos emergidos) e novembro (2.667 adultos emergidos), enquanto a menor foi em janeiro (203 adultos emergidos). Uma correlação positiva perfeita (r = +0,8) foi encontrada entre temperatura e população de mosquitos (DF 10 e α 0,05). O índice de diversidade de espécies de mosquitos permaneceu entre 0,12 e 1,76. Os componentes de riqueza de Margalef foram visivelmente baixos para armadilhas de bambu (0,2) e razoavelmente altos para campos de arroz, água percolada e rastros de animais (1,3), o que mostra a abundância de espécies de mosquitos nesses habitats. Da mesma forma, a uniformidade de Pielou foi maior para armadilhas de bambu (E = 1), mostrando distribuição uniforme das espécies. As pegadas de animais foram presumidas não apenas como habitat diverso, mas também possuíam alto valor para riqueza e uniformidade de espécies. Temperatura, chuva, umidade e outros atributos relacionados responsáveis pela variação e abundância das espécies precisam ser analisados mais a fundo para abrir caminho para o controle de espécies de vetores em seus locais de oviposição alvo. (Aedes Anopheles Armigeres Culiseta imersão anotada 1 42430 42 430 42.43 imaturos 41556 41 556 41.55 87 pupas Destes 19651 19 651 19.65 11512 11 512 11.51 8139 8 139 8.13 machos 78 n 15333 22 4318 examinada 79% 79 (79% prevalente 2.243 2243 243 (2.24 emergidos 2.667 2667 667 (2.66 emergidos, , 203 (20 emergidos. . r +0,8 08 0 DF 0,05. 005 0,05 05 0,05) 012 12 0,1 176 76 1,76 0,2 02 (0,2 arroz 1,3, 13 1,3 3 (1,3) forma E 1, 1) diverso Temperatura chuva 4243 4 43 42.4 4155 55 41.5 1965 65 19.6 1151 51 11.5 813 8.1 7 1533 431 (79 2.24 224 24 (2.2 2.66 266 66 (2.6 20 (2 +0, 00 0,0 01 0, 1,7 (0, (1,3 424 42. 415 5 41. 196 6 19. 115 11. 81 8. 153 (7 2.2 (2. 2.6 26 ( +0 (0 (1, 2. + (1

Abstract The work aims to analyze the associations of polymorphic variants of the PRL and BLG genes with resistance and susceptibility to mastitis in Holstein cows. The experimental study consisted of the selection of biomaterial samples from 250 heads of Holstein cows aged 3 years divided into two groups (healthy and with a confirmed diagnosis of mastitis). The determination of animal genotypes was carried out using polymerase chain reaction and restriction fragment length polymorphism. The study of the nature of the association of polymorphic variants of the PRL and BLG gene with resistance/increased risk of mastitis established a significant deviation from the theoretically expected distribution of bBLG-HaeIII genotypes in the group of animals suffering from mastitis (the value of χ2 was 0.24). The bBLG-HaeIIIBB genotype can act as a marker of an increased risk of developing mastitis in Holstein cows; its frequency in the group of sick animals exceeds the frequency in the control group by more than 2 times (44.0 compared to 17.0%, respectively). The bBLG-HaeIIIAB genotype is significantly associated with mastitis resistance in Holstein cows; its frequency is 2 times lower than in the control group (28.0 compared to 54.0%). 25 healthy mastitis. . mastitis) polymorphism resistanceincreased bBLGHaeIII bBLG HaeIII χ 0.24. 024 0.24 0 24 0.24) bBLGHaeIIIBB HaeIIIBB 44.0 440 44 (44. 170 17 17.0% respectively. respectively respectively) bBLGHaeIIIAB HaeIIIAB 28.0 280 28 (28. 54.0%. 540 54.0% 54 54.0%) 02 0.2 44. 4 (44 1 17.0 28. (28 54.0 5 0. (4 17. (2 54. (

Resumo O trabalho tem como objetivo analisar as associações de variantes polimórficas dos genes PRL e BLG com resistência e suscetibilidade à mastite em vacas holandesas. O estudo experimental consistiu na seleção de amostras de biomateriais de 250 cabeças de vacas holandesas com 3 anos de idade divididas em dois grupos (saudáveis e com diagnóstico confirmado de mastite). A determinação dos genótipos dos animais foi realizada utilizando reação em cadeia da polimerase e polimorfismo de comprimento de fragmentos de restrição. O estudo da natureza da associação de variantes polimórficas do gene PRL e BLG com resistência/risco aumentado de mastite estabeleceu um desvio significativo da distribuição teoricamente esperada dos genótipos bBLG-HaeIII no grupo de animais que sofrem de mastite (o valor de χ2 foi 0,24). O genótipo bBLG-HaeIIIBB pode atuar como marcador de risco aumentado de desenvolvimento de mastite em vacas holandesas; sua frequência no grupo de animais doentes excede a frequência no grupo controle em mais de duas vezes (44,0% contra 17,0%, respectivamente). O genótipo bBLG-HaeIIIAB está significativamente associado à resistência à mastite em vacas holandesas; sua frequência é duas vezes menor que no grupo controle (28,0% contra 54,0%). 25 saudáveis mastite. . mastite) restrição resistênciarisco bBLGHaeIII bBLG HaeIII o χ 0,24. 024 0,24 0 24 0,24) bBLGHaeIIIBB HaeIIIBB 44,0% 440 44 (44,0 170 17 17,0% respectivamente. respectivamente respectivamente) bBLGHaeIIIAB HaeIIIAB 28,0% 280 28 (28,0 54,0%. 540 54,0% 54 54,0%) 2 02 0,2 44,0 4 (44, 1 17,0 28,0 (28, 54,0 5 0, 44, (44 17, 28, (28 54, (4 (2 (

Resumo A raposa-vermelha (Vulpes vulpes) é um carnívoro de médio porte que ocorre em diferentes regiões do Paquistão, porém ainda carece de dados científicos sobre sua ecologia e distribuição. O presente estudo investigou o status filogenético e a dieta da raposa-vermelha (V.v. griffithii) que ocorre no Parque Nacional de Ayubia, Paquistão. Por meio de armadilhas fotográficas e análises moleculares, confirmamos a ocorrência de raposa-vermelha na área de estudo. Com base no citocromo B mitocondrial (304 bp) e amostragem limitada, quase todas as raposas-vermelhas do Parque Nacional de Ayubia e áreas circundantes do Himalaia se enquadram na linhagem materna holártica, enquanto as raposas-vermelhas encontradas nas planícies do Paquistão fazem parte da linhagem materna basal paleártica. Usando 32 fezes, descobrimos que a dieta da raposa-vermelha compreende 80% de espécies de presas de origem animal (selvagens e domésticas) e 19% de matéria vegetal. As espécies de presas de animais selvagens incluíram a lebre-do-cabo (Lepus capensis) e o esquilo-voador (Pteromyini sp.), que constituíram 17% e 15% da dieta, respectivamente. As raposas-vermelhas consumiam raramente ratos domésticos (Mus musculus), algas do Himalaia (Paguma larvata) e ovelhas (Ovis aries), cada um compreendendo cerca de 6% a 9% da dieta da raposa-vermelha. A espécie de raposa também se alimentava de burros domésticos de forma oportunista. Com base em nossa amostragem, nosso estudo sugere que a raposa-vermelha (V.v. griffithii) que ocorre no Parque Nacional de Ayubia e nas cordilheiras menores do Himalaia pertence à linhagem materna holártica. O estudo também destaca o consumo de sementes de plantas por raposas-vermelhas, indicando que pode desempenhar um papel ecológico importante na dispersão de sementes no Parque Nacional de Ayubia.

Abstract The red fox (Vulpes vulpes) is a medium-sized carnivore that occurs in different regions of Pakistan, however, still lacks scientific data on its ecology and distribution. The current study investigated the phylogenetic status and diet of the red fox (V.v. griffithii) occurring in Ayubia National Park, Pakistan. Through camera trapping and molecular analysis, we confirmed the occurrence of red fox in the study area. Based on mitochondrial cytochrome B (304 bp) and limited sampling, nearly all red foxes of Ayubia National Park and surrounding Himalayan ranges fall within Holarctic maternal lineage, whereas red foxes found in plains of Pakistan are part of the basal Palearctic maternal lineage. Using 32 scats, we found that red fox diet comprises of 80% animal-based prey species (both wild and domestic) and 19% plant matter. The wild animal prey species included Cape hare (Lepus capensis) and flying squirrel (Pteromyini sp.), which constituted 17% and 15% of diet, respectively. Red foxes infrequently consumed House mouse (Mus musculus), Himalayan Palm civet (Paguma larvata) and sheep (Ovis aries), each comprising around 6% to 9% of red fox diet. The fox species also scavenged on domestic donkey opportunistically. Based on our sampling, our study suggests that the red fox (V.v. griffithii) that occurs in Ayubia National Park and across the lesser Himalayan ranges belongs to Holarctic maternal lineage. The study also highlights consumption of plant seeds by red foxes, indicating it may play an important ecological role in seed dispersal in Ayubia National Park.

Resumo O efeito de borda tem impactos severos na sobrevivência de sementes e plântulas devido a modificações dos fatores bióticos e abióticos. Frequentemente, espécies arbóreas com sementes grandes perdem seus dispersores na borda da floresta devido à raridade ou ausência de grandes frugívoros neste tipo de habitat. Neste estudo, comparei a abundância e distribuição de plântulas de S. flexuosa entre bordas e interiores de três grandes remanescentes de Cerrado. Em cada remanescente, o número de plântulas ao redor das palmeiras-mãe, na borda, era menor do que ao redor dos indivíduos no interior do Cerrado. Nas bordas, a maioria das plântulas foi encontrada junto às plantas mãe, enquanto no interior ocorreu o contrário. A alta concentração de plântulas sob as plantas adultas sugere diminuição da dispersão de sementes nas bordas. Como S. flexuosa é uma palmeira amplamente distribuída que serve como um recurso importante para vários animais nos habitats do Cerrado, mudanças no processo de regeneração dessa palmeira devido aos efeitos de borda podem impactar ainda mais as populações de frugívoros. Portanto, o declínio do estabelecimento de plântulas ao longo das bordas do Cerrado implica em mudanças na dinâmica de regeneração do Cerrado, o que pode comprometer a persistência de processos ecológicos e comunidades animais.

Abstract The edge effect has impacts on seed and seedling survival due to modifications in biotic and abiotic factors. Often, large-seeded tree species lost seed vectors in the forest edge due to the rarity or absence of large frugivores at this habitat type. In this study, I compared the seedling abundance and distribution of the palm Syagrus flexuosa between edges and interiors of three large Cerrado remnants. In every remnant, the number of seedlings around parent palms in the edge was smaller than around palm individuals located in the Cerrado interior. Moreover, the distribution of seedlings around parent palms differed between edges and interiors. In the edges, most seedlings were found under parent crowns, while in the interiors, the contrary occurred. The high concentration of seedlings under parent palms suggests a decrease of seed dispersal at the edges. Because S. flexuosa is a widely distributed palm that serves as an important resource for several animals along Cerrado habitats, changes on the regeneration process of this palm due to edge effects can further impact frugivore populations. Therefore, the decline of seedling establishment along forest edges implies changes in the Cerrado regeneration dynamics, which may compromise the persistence of ecological processes and animal communities.

ABSTRACT The ordinary least squares (OLS) can be affected by errors associated with heteroscedasticity and outliers, and extreme points can influence the regression parameters. Methods based on the median rather than on the mean and variance are more resistant to outliers and extreme points. These methods could be used to obtain regression parameter estimates that reflect more accurately the genuine relationship between the Y and X variables, leading to better identification of outliers and extreme points by comparing the slopes and intercepts of both methods. The Theil-Sen (TS) regression computes all possible pairwise slopes and determines the median of slopes as the regression slope. Here, we illustrated the potential use of TS and frequently used robust regression (RR) techniques to single linear regression using synthetic datasets and a practical problem in animal science. Three synthetic datasets were created assuming the normal distribution of Y and X values: one was free of outliers, while the other two had one or two clusters of outliers but the same X values. The TS, OLS, and RR had nearly identical regression parameter estimates for the dataset without synthetic outliers. However, the intercept and slope estimates by the OLS method differed considerably from the TS and RR methods when one or two clusters of outliers were included. The TS approach could be used to indirectly determine the presence of outliers or extreme points by comparing the 95 % confidence interval of the TS and OLS parameter estimates. (OLS parameters variables TheilSen Theil Sen (TS Here (RR science values However included 9

ABSTRACT The objective of this study was to analyze data of the genus Melocactus (Cactaceae) in the Caatinga biome using a virtual herbarium, determine its reproductive aspects, and establish its geographical distribution. Data were collected from the SpeciesLink platform. Notes on exsiccata labels were analyzed to assess reproductive characteristics. Coordinate information was used to develop a distribution map for the genus in the Caatinga biome. A total of 469 records were found in 35 herbaria in Brazil. Melocactus zehntneri stood out with 208 records, followed by M. ernestii, and M. bahiensis. The data were concentrated in a few herbaria, mainly in the Herbarium of the State University of Feira de Santana, which had 28% of the records. Collections were carried out in June (63 records) and in July (59 records). Regarding the reproductive period, Melocactus was active throughout the year, but more concentrated from June to October. The genus is composed of species that depend on animal pollination and dispersal, according to coloration and visitation data. Melocactus is widely distributed throughout the Caatinga, with a tendency to concentrated in drier areas of the biome, in the states of Bahia, Pernambuco, and Paraiba. Herbaria are valuable tools for ecological investigations; therefore, promoting the digitalization of collections and improving the quality and quantity of annotations on specimens are necessary. The genus Melocactus has several characteristics that make it an essential group for maintaining biodiversity in the Caatinga biome. Cactaceae (Cactaceae herbarium aspects platform 46 3 Brazil 20 M ernestii bahiensis Santana 28 63 (6 59 (5 . period year October dispersal Bahia Pernambuco Paraiba investigations therefore necessary 4 2 6 ( 5

RESUMO O objetivo do estudo foi analisar os dados do gênero Melocactus (Caatinga) na Caatinga em herbário virtual e avaliar seus aspectos reprodutivos e estabelecer a sua distribuição geográfica. Para tanto, foram coletadas informações na plataforma SpeciesLink. As exsicatas foram analisadas quanto às notas em suas etiquetas para a avaliação dos aspectos reprodutivos. A partir das informações das coordenadas, foi elaborado um mapa da distribuição do gênero ao longo da Caatinga. Um total de 469 registros foram encontrados em 35 herbários brasileiros. Melocactus zehntneri se destacou com 208 registros, seguido por M. ernestii, e M. bahiensis. Os dados estiveram concentrados em poucos herbários, com destaque ao HUEFS, que liderou com 28% dos registros. Verificou-se 63 registros nos meses de junho e 59 em julho, referentes à quantidade de coletas realizada por mês. Em período reprodutivo Melocactus foi ativo ao longo do ano inteiro, porém com concentração de junho a outubro. O gênero é composto por espécies que dependem da polinização e da dispersão realizada por animais, de acordo com os dados de coloração e de visitação. Melocactus é amplamente distribuído pela Caatinga, com tendência de concentração em áreas mais secas dentro do bioma, nos estados da Bahia, Pernambuco e Paraíba. Os herbários são ferramentas valiosas para investigações ecológicas, portanto é necessário promover a digitalização dos acervos, bem como melhorar a qualidade e quantidade das anotações sobre os espécimes. O gênero Melocactus apresenta diversas características que o torna um grupo essencial para a manutenção da biodiversidade da Caatinga. (Caatinga geográfica tanto SpeciesLink coordenadas 46 3 brasileiros 20 M ernestii bahiensis HUEFS 28 Verificouse Verificou 6 5 julho mês inteiro outubro animais visitação bioma Bahia Paraíba ecológicas acervos espécimes 4 2

ABSTRACT. The aim of the present study was to evaluate the dietary effect of an enzymatic complex on the growth performance variables, blood parameters, and economic viability of piglets. To achieve this, we used 80 piglets (40 castrated males and 40 females) in a 2 × 2 factorial design following a randomized block experimental distribution, with two levels of metabolizable energy (adequate: 3206.09 and low: 3005.45 kcal kg-1) and two enzyme levels (0 and 50 g ton-1). The results showed no significant difference between treatments in the growth performance variables or blood parameters of piglets. The economic viability, economic efficiency index, and cost index were improved when we used a diet with a low energy supplemented with the enzyme complex as compared to other experimental diets. Thus, we concluded that enzyme complex supplementation can maintain growth performance and blood parameters in piglets even when they are fed low energy diets. Moreover, this could reduce production costs. ABSTRACT 8 (4 4 females distribution adequate (adequate 320609 3206 09 3206.0 300545 3005 45 3005.4 kg1 kg 1 kg-1 0 ( 5 ton1. ton1 ton . ton-1) diets Thus Moreover costs 32060 320 3206. 30054 300 3005. kg- ton-1 32 30 ton- 3

ABSTRACT In this study, optimization models for the production and logistics tactical planning of the corn supply chain are presented and several outputs are explored. They consider the domestic market for animal consumption, ethanol production, and the international market. They are mainly oriented to support policies and decisions of public agents who have some interference in the supply chain. To evaluate the consistency and effectiveness of the models, they were applied to solve realistic problem instances of the Brazilian corn supply chain considering different scenarios and using data collected from several sources. The outcomes demonstrated that models can produce analysis considering aspects that have not been fully addressed in the literature before, such as some opportunities to use different modes of transport for corn distribution, to contract extra capacity for stocking and the impacts of some state taxes on its production and logistic systems. Other important aspects explored in the scenario analysis are the identification of capacity bottlenecks and investment opportunities. The conclusions and insights are converted into practical interpretations to support relevant supply chain decisions. study consumption sources before distribution systems

ABSTRACT Since prehistoric times, indigenous communities have relied on fish as a staple source of protein for their subsistence. In most cases, fish is the sole source of income and of animal protein for the communities. Nevertheless, the documentation of fish from an ethnological perspective is rare, and biological research alone might not provide sufficient information required to manage fisheries resources. We discuss the traditional ichthyological knowledge of the Piapoco, Piaroa, Puinave, and Sikuani ethnic groups inhabiting the Matavén Forest (Vichada, Colombia). Each group has its perception regarding knowledge, at times detailed, of fish groups, their distribution, and uses. To obtain information regarding traditional ichthyological knowledge, fish were collected over one month at 31 sampling sites in different habitats along the lower Matavén River basin. The fish were identified by formal taxonomy and the informal classification by experienced fishermen of the four ethnic groups. There was consistency among the names given by the ethnic groups and formal taxonomy. The indigenous collaborators used binomial classification systems in which the organisms are grouped at family and genus levels and, to some extent, categorization depends on distribution within the aquatic habitats (i.e., rivers, streams and lagoons). The indigenous collaborators did not provide sociocultural or mythological information related to the fishes. Our findings contribute to the development of conservation and rural development projects in the Colombian Amazon. subsistence cases Nevertheless rare resources Piapoco Piaroa Puinave Vichada, Vichada (Vichada Colombia. Colombia . Colombia) detailed uses 3 basin extent i.e., ie i e (i.e. rivers lagoons. lagoons lagoons) fishes Amazon i.e. (i.e i.e

RESUMEN Desde la prehistoria, las comunidades indígenas han dependido del pescado como fuente primaria de proteína para su subsistencia. En la mayoría de los casos, el pescado es la única fuente de ingresos y proteína animal para la comunidad. Sin embargo, el estudio de los peces desde una perspectiva etnológica no es común y la investigación científica por sí sola puede no proporcionar información suficiente para gestionar los recursos pesqueros. Se discute aquí el conocimiento ictiológico tradicional de las etnias Piapoco, Piaroa, Puinave y Sikuani que habitan la Selva de Matavén (Vichada, Colombia). Cada etnia tiene una percepción propia, a veces detallada, de los grupos de peces, su distribución y usos. Para obtener información sobre el conocimiento ictiológico tradicional, se colectaron peces durante un mes en 31 sitios de muestreo en diferentes hábitats en la cuenca baja del Río Matavén. Los peces fueron identificados usando taxonomía formal y clasificación informal por pescadores expertos de las cuatro etnias. Hubo coherencia entre los nombres dados por los grupos étnicos y el sistema de taxonomía formal. Los colaboradores indígenas usaron sistemas de clasificación binomial en los que los organismos se agrupan a nivel de familia y género y la categorización depende, hasta cierto punto, de la distribución dentro de los hábitats acuáticos (ríos, arroyos y lagunas). Los colaboradores indígenas no proporcionaron información sociocultural o mitológica relacionada con los peces. Nuestros hallazgos contribuyen al desarrollo de proyectos de conservación y desarrollo rural en la Amazonía colombiana. prehistoria subsistencia casos comunidad embargo pesqueros Piapoco Piaroa Vichada, Vichada (Vichada Colombia. Colombia . Colombia) propia detallada usos 3 depende punto ríos, ríos (ríos lagunas. lagunas lagunas) colombiana

Abstract The objectives were to evaluate the effects of recipient category, season and administration of a gonadorelin analogue at the time of fixed-time embryo transfer (FTET) on pregnancy per FTET at 30 and 60 days after oestrus and on pregnancy losses (PL). Recipients were randomly assigned to: treated group (n = 624), in which recipients received an intramuscular injection of 0.2 mg of gonadorelin (Fertagyl®) at FTET; or a control group (n = 687) that remained untreated. Recipients were previously treated with a synchronisation protocol based on progesterone and oestradiol. All embryos were produced in vitro. The data with binomial distribution were analysed by multivariate logistic regression, using the GLIMIX procedure of SAS. Higher P/FTET was observed at 30 days (45.8 vs. 40.0%; P = 0.03) and 60 days (43.0 vs. 37.0%; P = 0.01) in the treated group. There was a tendency toward reduced PL in the treated group (4.0 vs. 7.0%; P = 0.09). Dry cows (2.70%) and lactating cows (2.47%) had less PL (P = 0.001) compared with heifers (10.42%). In the spring/summer season the P/ FTET at 60 days was smaller (P =0.024). Greater PL tended to occur in the warmer season. Treatment with gonadorelin at the time of bovine ET increased the pregnancy per ET at days 30 and 60 and reduced PL. Additionally, dry and lactating recipient cows showed a lower PL rate compared to heifers. Furthermore, ET performed in the warmer seasons of the year resulted in a lower pregnancy rate at day 60 and greater PL. category fixedtime fixed (FTET 3 6 . (PL) n 624, 624 , 624) 02 0 2 0. Fertagyl® Fertagyl (Fertagyl® 687 untreated oestradiol vitro regression SAS PFTET 45.8 458 45 8 (45. vs 40.0% 400 40 0.03 003 03 43.0 430 43 (43. 37.0% 370 37 0.01 001 01 4.0 4 (4. 7.0% 70 7 0.09. 009 0.09 09 0.09) 2.70% 270 (2.70% 2.47% 247 47 (2.47% 0.001 0001 10.42%. 1042 10.42% 10 42 (10.42%) springsummer spring summer =0.024. 0024 =0.024 024 =0.024) Additionally Furthermore (PL 62 (Fertagyl 68 45. (45 40.0 0.0 00 43. (43 37.0 4. (4 7.0 2.70 27 (2.70 2.47 24 (2.47 0.00 000 104 10.42 1 (10.42% 002 =0.02 40. 37. ( 7. 2.7 (2.7 2.4 (2.4 10.4 (10.42 =0.0 2. (2. 10. (10.4 =0. (2 (10. =0 (10 (1

Resumo Objetivou-se avaliar os efeitos da administração de análogo da gonadorelina no momento da transferência de embrião em tempo fixo (TETF), da categoria da receptora e da estação do ano na prenhez por TETF aos 30 (P/TETF 30) e 60 (P/ TETF60) após o estro e nas perdas gestacionais (PG). As receptoras foram distribuídas aleatoriamente: grupo tratado (n = 624), onde as receptoras receberam injeção intramuscular de 0,2 mg de gonadorelina (Fertagyl®) na TETF e grupo controle (n =687) permaneceu sem tratamento. As receptoras foram previamente tratadas com protocolo de sincronização à base de progesterona e estradiol. Todos os embriões foram produzidos in vitro. Os dados foram analisados por regressão logística multivariada, utilizando o procedimento GLIMIX do SAS. Foi detectada maior P/TETF30 (45,8 vs. 40,0%; P =0,03) e P/TETF60 (43,0 vs. 37,0%; P =0,01) no grupo tratado. Houve tendência de redução da PG no grupo tratado (4,0 vs. 7,0%; P =0,09). Vacas secas (2,70%) e vacas em lactação (2,47%) apresentaram menor PG (P =0,001), em comparação com novilhas (10,42%). Na estação primavera/verão a P/TETF60 foi menor (P = 0,024). Maior PG tendeu a ocorrer na estação mais quente. O tratamento com gonadorelina no momento do TETF aumentou a prenhez por TE aos dias 30 e 60 e reduziu o PG. Vacas receptoras secas e lactantes apresentaram menor taxa de PG em comparação com novilhas. Além disso, a TE realizada nas estações mais quentes do ano resultou em menor taxa de prenhez aos 60 dias e maior PG. Objetivouse Objetivou se TETF, , (TETF) 3 P/TETF PTETF 6 P/ TETF60 . (PG) aleatoriamente n 624, 624 624) 02 0 2 0, Fertagyl® Fertagyl (Fertagyl® =687 687 estradiol vitro multivariada SAS PTETF30 TETF30 P/TETF3 45,8 458 45 8 (45, vs 40,0% 400 40 =0,03 003 03 PTETF60 P/TETF6 43,0 430 43 (43, 37,0% 370 37 =0,01 001 01 4,0 4 (4, 7,0% 70 7 =0,09. 009 =0,09 09 =0,09) 2,70% 270 (2,70% 2,47% 247 47 (2,47% =0,001, 0001 =0,001 =0,001) 10,42%. 1042 10,42% 10 42 (10,42%) primaveraverão primavera verão 0,024. 0024 0,024 024 0,024) quente disso (TETF TETF6 (PG 62 (Fertagyl =68 68 PTETF3 TETF3 45, (45 40,0 =0,0 00 PTETF6 43, (43 37,0 4, (4 7,0 2,70 27 (2,70 2,47 24 (2,47 000 =0,00 104 10,42 1 (10,42% 002 0,02 =6 40, =0, 37, ( 7, 2,7 (2,7 2,4 (2,4 10,4 (10,42 0,0 =0 2, (2, 10, (10,4 (2 (10, (10 (1

Abstract The three-toed sloth (Bradypus variegatus) has been a victim of disorderly human interventions, leading to an increase in accidents and diseases for the species. This study aimed to provide a description of the coronary arteries and to high light the histopathological findings in the sloth’s heart. This information should be beneficial for medical clinics dealing with these species. Eighteen B. variegatus specimens were dissected after natural death. Fifteen of them, comprising five adult males, nine adult females, and one juvenile, were fixed with formaldehyde and preserved in saline solution. In two males and one female, red-coloured latex was injected through the left common carotid artery to make the coronary arteries more visible. In the female, blue-coloured latex was also injected through the right external jugular vein to aid in arterial identification. The chests were opened to evaluate and extract the heart for a detailed description of the coronary arteries. For histopathological analysis, histological slides were prepared from four hearts collected from four animals (three adults and one juvenile). Arterial analyses revealed that sloths possess two coronary arteries: one on the right and one on the left side, each of which supplies blood to their respective surfaces. The heart is composed of the endocardium, myocardium, and epicardium, similar to mammals in general. However, necrosis of endocardial tissue and cardiac muscle fbres, as well as myxomatous degeneration of valves and thrombi in vascular lumens, were observed. These necrotic events, more extensive in nature, are related to infarctions, while the more subtle ones may be associated with stressful situations experienced by the individuals. threetoed three toed Bradypus interventions species s B death them females juvenile solution female redcoloured red coloured visible bluecoloured blue identification analysis juvenile. . juvenile) side surfaces endocardium myocardium epicardium general However fbres lumens observed events nature infarctions individuals

Resumo A preguiça-comum (Bradypus variegatus) tem sido vítima das investidas antrópicas desordenadas, o que tem elevado o número de acidentes e doenças para a espécie. Propôs-se com esse estudo, realizar uma descrição das artérias coronárias, assim como apontar os achados histopatológicos do coração do bicho-preguiça, a fim de favorecer o diagnóstico por imagem, semiologia e clínica médica das espécies. Foram dissecados 18 exemplares de B. variegatus obtidos após morte natural. Quinze deles, sendo 5 machos e 9 fêmeas adultas e 1 jovem, foram fixados com formaldeído e conservados em solução salina. Em 2 machos e 1 fêmea, foi injetado, a partir da artéria carótida comum esquerda, látex corado em vermelho, para tornar as coronárias mais evidentes. Na fêmea também foi injetado através da veia jugular externa direita, látex corado em azul, para evitar equívoco na identificação arterial. Os animais tiveram o tórax aberto a fim de avaliar e retirar o coração para descrição detalhada das artérias coronárias. Para análise histopatológica, foram confeccionadas lâminas histológicas oriundas de corações coletados de 4 animais (3 adultos e 1 jovem). Com base nas análises arteriais, as preguiças possuem duas coronárias: uma a direita e uma a esquerda, que irrigam suas respectivas faces. O coração é composto por endocárdio, miocárdio e epicárdio, semelhante aos mamíferos em geral. Todavia, observou-se necroses endocárdicas, de fibras musculares cardíacas, além de degenerações mixomatosas de válvulas e trombos no lúmen vascular. Esses eventos necróticos, mais extensos, estão relacionados a infartos, enquanto os mais discretos podem ser associados a situações de estresse vivenciadas pelos espécimes. preguiçacomum preguiça Bradypus desordenadas espécie Propôsse Propôs se estudo bichopreguiça, bichopreguiça bicho preguiça, bicho-preguiça imagem espécies B natural deles jovem salina esquerda vermelho evidentes azul arterial histopatológica 3 ( jovem. . jovem) arteriais faces endocárdio epicárdio geral Todavia observouse observou endocárdicas cardíacas vascular necróticos extensos infartos espécimes