ABSTRACT The feeding habits of the bonito Euthynnus alletteratus and the albacore Thunnus alalunga were assessed by analyzing the contents of 85 stomachs (50 from bonito and 35 from albacore) collected between March 2018 and April 2019. A qualitative and quantitative analysis of the prey found in the stomach contents was conducted, as well as of the trophic spectrum between species and climatic seasons. Climatic information was obtained through satellite images, analyzing variables such as sea surface temperature, salinity, and winds. In terms of abundance, the most representative prey were the fish Sardinella aurita (42.48 %) and Teleostei remains (27.45 %) for E. alletteratus, and Megalopa larvae (56.89 %) and Teleostei remains (10.26 %) for T. alalunga. The relative importance index indicates that the main prey for E. alletteratus were Teleostei remains (IRI = 1460.65) and Sardinella aurita (IRI = 1179.70), while, for T. alalunga they were megalopa larvae (IRI = 1367.19) and Teleostei remains (IRI = 840.94). These large pelagic predators were classified as generalists, with a low prey diversity in their stomach content. No trophic overlap was observed between the two species.

RESUMEN Se evaluaron los hábitos alimentarios del bonito Euthynnus alletteratus y la albacora Thunnus alalunga a partir del análisis de los contenidos de 85 estómagos (50 de bonito y 35 de albacora) recolectados entre marzo de 2018 y abril de 2019. Se realizó un análisis cualitativo y cuantitativo de las presas encontradas en dichos estómagos, así como del espectro trófico entre especies y temporadas climáticas. La información climática se obtuvo a través de imágenes satelitales, analizando variables como la temperatura superficial del mar, la salinidad y los vientos. En términos de abundancia, las presas más representativas fueron el pez Sardinella aurita (42.48 %) y restos de Teleostei (27.45 %) para E. alletteratus, y larvas megalopa (56.89 %) y restos de Teleostei (10.26 %) para T. alalunga. El índice de importancia relativa indica, como presas principales, a restos de Teleostei (IRI = 1460.65) y Sardinella aurita (IRI = 1179.70) para E. alletteratus, y a larvas megalopa (IRI = 1367.19) y restos de Teleostei (IRI = 840.94) para T. alalunga. Estos grandes depredadores pelágicos fueron catalogados como generalistas, con baja diversidad de presas en el contenido estomacal, y no se observó solapamiento trófico entre las dos especies.

Abstract The present study addresses the identification and estimation of siliceous diagnostic microremains of maize (Zea mays) in archaeological contexts of North-east Argentina, contributing to the understanding of pre-Hispanic agricultural expansion in the region. The methodology involved the application of processing protocols for current and archaeological plants and sediments, establishing correlations between samples. The result of this process allowed us to calculate the number of cross-shaped morphotypes per gram of dry material in contemporary maize leaves, key information to estimate the relative abundance of diagnostic elements in archaeological cultivation areas, providing an estimation index applicable to other contexts. This analysis not only reveals the ubiquity of Zea mays at sites, but also provides a measure to estimate the approximate volume of its production at archaeological sites, addressing outstanding questions about exchange routes, horticultural modalities and needs of human groups in the past.

Resumen El presente estudio aborda la identificación y estimación de microrrestos silíceos diagnósticos de maíz (Zea mays) en plantas actuales y sitios arqueológicos del Noreste argentino, contribuyendo a la comprensión de la expansión agrícola prehispánica en la región. La metodología involucró la aplicación de protocolos de procesamiento para plantas y sedimentos actuales y arqueológicos, estableciendo correlaciones entre las muestras. El resultado de este proceso permitió calcular la cantidad de morfotipos en forma de cruz por gramo de material seco en hojas de maíz actual, información clave para estimar la abundancia relativa de elementos diagnósticos en áreas de cultivo arqueológicas, proporcionando un índice de estimación aplicable a otros contextos. Este análisis no solo revela la ubicuidad de Zea mays en los sitios, sino que también ofrece una medida para calcular el volumen aproximado de su producción en sitios arqueológicos, abordando preguntas pendientes sobre rutas de intercambio, modalidades hortícolas y necesidades y demandas de los grupos humanos en el pasado.

RESUMEN Las investigaciones sobre cianobacterias bentónicas en reservorios de alta montaña en regiones tropicales aún son limitadas, debido a su complejidad y vacíos de conocimiento en relación con su ecología. Este estudio tuvo como objetivo explorar la composición, diversidad y potencial tóxico de estas cianobacterias en sedimentos superiores de dos embalses colombianos, empleando múltiples metodologías, como experimentos de germinación para evaluar su presencia y viabilidad en sedimentos, ensayos de ELISA competitivo para cuantificar toxinas en cultivo, análisis de Unidades Taxonómicas Operativas (OTUs) a partir de submuestras de los cultivos para evaluar su diversidad y riqueza entre los diferentes phylum procarióticos, y análisis filogenético de cianobacterias cultivables. Se confirmó la presencia de poblaciones viables de cianobacterias en los sedimentos mediante mediciones de clorofila-a y la identificación microscópica demostró su capacidad de crecimiento en condiciones controladas de laboratorio. A pesar de los bajos niveles de microcistina en cultivo, la detección previa de genes mcy en muestras directas de sedimento, sugiere un potencial tóxico en las cianobacterias presentes. El análisis de la comunidad reveló una notable diversidad microbiana en los sedimentos de ambos embalses, con una mayor abundancia relativa de cianobacterias en comparación con otros phylum procarióticos. Estos hallazgos respaldan la hipótesis de que los sedimentos superficiales desempeñan un papel fundamental como depósito de cianobacterias que pueden plantear riesgos inherentes a la salud del ecosistema. En conclusión, esta investigación subraya la necesidad de realizar más estudios para lograr una comprensión holística de la dinámica de las cianobacterias bentónicas en reservorios tropicales de alta montaña.

ABSTRACT Benthic cyanobacteria research in high mountain reservoirs remains limited, mainly due to their complexity and knowledge gaps that persist in relation to their ecology in tropical regions. This study aimed to explore the composition, diversity, and toxic potential of cyanobacterial in the upper sediment of two Colombian reservoirs. Our investigation involved multiple methodologies, such as germination experiments, that allowed us to assess the presence and viability of cyanobacteria in upper sediment samples, while the competitive ELISA assay allowed for the quantification of toxins within the cultures. The molecular analysis of Operational Taxonomic Units (OTUs) from subsamples of sediment cultures focused on evaluating cyanobacterial diversity and richness among prokaryotic phyla, and the Phylogenetic analysis of culturable cyanobacteria. Chlorophyll-a measurements confirmed the presence of viable populations in sediment cultures, while microscopic identification demonstrated the growth capacity of cyanobacteria from the orders Nostocales, Chroococcales, Oscillatoriales, and Synechococcales under controlled laboratory conditions. Despite low microcystin levels in culture, the prior detection of mcy genes in direct sediment samples suggests a possible toxic potential of cyanobacterial inhabiting the upper sediments. Community analysis, based on the OTUs abundance, revealed a notably diverse microbial community in both reservoir sediments, with a higher relative abundance of cyanobacteria compared to other prokaryotic phyla. These findings support the hypothesis that surface sediments play a fundamental role as a repository for cyanobacteria that may pose inherent risks to ecosystem health. In conclusion, this research underscores the necessity of further studies to achieve a holistic comprehension of benthic cyanobacteria dynamics in high-mountain tropical reservoirs.

ABSTRACT: The zooplankton species present in Argopecten purpuratus “fan shell” culture areas were determined in Vichayo, Sechura, Piura; The physical and chemical parameters were related to the composition of the zooplankton; The sampling unit was 100 ml of water, in which zooplankton, temperature, pH, oxygen and salinity were evaluated. 180 samples were collected from 5 sampling points, chosen at random, using drag and vertical sampling, for six months. ; species are determined using taxonomic keys and the appropriate equipment to evaluate physical and chemical factors. The data obtained were analyzed using descriptive and inferential statistics, with decisions of 95% confidence. The results indicate that the phyllum Arthropoda predominates in the zooplankton and within it, the Copepoda class; The physical and chemical values were within the established ranges. Concluding that of 100% of individuals of the zooplankton species community present in the evaluated areas, 69.84% of them belong to the Phylum Arthropoda; and that the zooplankton community remains in dynamic equilibrium due to low dominance and high values of species richness, diversity and equitability.

RESUMEN: Se determinaron las especies de zooplancton presentes en áreas de cultivo de Argopecten purpuratus “concha de abanico” Vichayo, Sechura, Piura; se relacionaron los parámetros físicos y químicos con la composición del zooplancton; la unidad muestral fue de 100 ml de agua, en la que se evaluó zooplancton, temperatura, pH, oxígeno y salinidad, fueron colectadas 180 muestras en 5 puntos de muestreo elegidos al azar, muestreos por arrastre y vertical durante seis meses; determinándose las especies con el uso de claves taxonómicas y oxímetro, peachímetro y refractómetro para evaluar factores físicos y químicos. Los datos obtenidos fueron analizados mediante estadística descriptiva e inferencial, con decisiones de 95% de confianza. Los resultados indican que en el zooplancton predomina el phyllum Arthropoda y dentro de él, la clase Copepoda; los valores físicos y químicos se hallaron dentro de los rangos establecidos. Concluyéndose que del 100% de las especies de zooplancton presentes en las áreas evaluadas, el 69,84% pertenecen al phylum Arthropoda; y que, la comunidad de zooplancton se mantiene en equilibrio dinámico debido a una baja dominancia y valores elevados de riqueza de especies, diversidad y equitabilidad.

Abstract The fragmentation of natural ecosystems has become a serious global problem caused by the construction of conventional infrastructure. While this development has contributed to the economic growth of large cities, its installation and operation have socio-economic impacts on various rural populations, creating conditions and feelings of marginalization and poverty among them. Therefore, an evaluation has been conducted using landscape ecology and specialized software to assess whether the infrastructure built since 1975 in the highlands of the Tacna Department has had ecological effects on its ecosystems and high Andean societies. It has been determined that 267.3 km2 of conventional infrastructure (5.51% of the total surface) has caused a barrier effect on the Suri and an area effect on various wild plant species, reducing their abundance, diversity, and coverage. Additionally, there is evidence of the edge effect, as indicated by changes in relative humidity and soil and ambient temperatures. The interaction of rural people with their natural environment fulfills their human needs, and it has been observed that the partial or total replacement of ecosystems has limited essential development conditions, such as access to resources and exclusion from decision-making about them. This replacements have generated a climate of exclusion and injustice, leading to protests and opposition in past social conflicts that still linger. Therefore, implementing better mechanisms for environmental impact assessment, territorial management, restoration, and rural development based on the conservation of ecosystems and adequate compensation for ecosystem services will ensure the development and social peace in the high Andean societies of the Puna ecoregion.

Resumen La fragmentación de los ecosistemas naturales viene siendo un grave problema mundial, ocasionado por la construcción de infraestructura convencional, que, si bien ha contribuido al desarrollo económico de las grandes ciudades, su instalación y funcionamiento afecta socioeconómicamente a diferentes poblaciones rurales, creando en ellas condiciones y sensaciones de marginalidad y pobreza. Por ello, se ha evaluado mediante la ecología del paisaje y un software especializado, si aquella construida desde el año 1975 en la puna del Departamento de Tacna, ha provocado efectos ecológicos sobre sus ecosistemas y sociedades altoandinas, determinándose que 267,3 km2 de infraestructura convencional (5.51% de la superficie total) ha provocado el efecto barrera sobre el Suri y el efecto área en diversas especies de flora silvestre, reduciendo su abundancia, diversidad y cobertura, pero también del efecto borde, evidenciado por cambios en la humedad relativa y temperaturas del suelo y ambiente encontradas. La interacción del hombre rural con su entorno natural satisface sus necesidades humanas, comprobándose .- que el reemplazo parcial o total de los ecosistemas, ha limitado las condiciones básicas de desarrollo, como el acceso a recursos y la exclusión 110 en las decisiones sobre ellos, generando un clima de injusticia, provocando reclamos y oposiciones durante los conflictos sociales pasados que aún son latentes, por lo que la implementación de mejores mecanismos de evaluación del impacto ambiental, de gestión territorial, de restauración y desarrollo rural, basado en la conservación de sus ecosistemas y las compensaciones o retribuciones efectivas por servicios ecosistémicos, garantizaran el desarrollo y la paz social en las sociedades altoandinas de la ecorregión Puna.

Abstract Sustainable forest management requires the integration of better practices for the conservation of biological diversity. Therefore, it is necessary to know the composition and richness of wild communities in sites where different forest management practices are applied. To this end, the fauna of managed forests in the Chignahuapan region of Puebla State was sampled for four years. A total of 112 species were recorded: 13 medium and large mammals, 83 birds, four amphibians and 12 reptiles. The abundances of these observed species were used to calculate the expected richness with the non-parametric Chao1 estimator. The management method with the highest expected richness was the Silvicultural Development Method (MDS) with 113, followed by Successive Cutting (CS) with 97, and the lowest was observed in the Mexican Method for the Management of Irregular Forests (MMOBI) with 71. Sampling completeness ranged from 92 % in amphibians to 99 % in mammals. Sixteen species are in some risk category in the official Mexican standard NOM-059-SEMARNAT-2010, and 25 are endemic to Mexico. Taxonomic lists contain information on conservation status, endemism and relative abundance. Intensive forest management methods, such as MDS, have a richer mammal and bird community than MMOBI and CS but are similar to protected areas. The richness and abundance of amphibians and reptiles, most of them in some risk category, are low, especially in MMOBI and CS, and almost absent in protected areas.

Resumen El manejo forestal sustentable requiere de la integración de mejores prácticas de conservación de la diversidad biológica. Para esto, es necesario conocer la composición y riqueza de las comunidades silvestres en los sitios donde se aplican diferentes prácticas silvícolas. Con esta finalidad, se muestreó durante cuatro años la fauna de bosques productivos de la región de Chignahuapan, Puebla. Se registraron 112 especies: 13 de mamíferos medianos y grandes, 83 de aves, cuatro de anfibios y 12 de reptiles. A partir de la abundancia de estas especies observadas, se determinó la riqueza esperada con el estimador no paramétrico Chao1. El método de manejo con mayor riqueza esperada fue el Método de Desarrollo Silvícola (MDS) con 113, seguido por Cortas Sucesivas (CS) con 97, y la menor se observó en el Método Mexicano de Ordenación de Bosques Irregulares (MMOBI) con 71. La completitud de los muestreos varió del 92 % en anfibios al 99 % en mamíferos. Dieciséis especies se encuentran en alguna categoría de riesgo en la norma oficial mexicana NOM-059-SEMARNAT-2010, y 25 son endémicas de México. Se presentan listas taxonómicas con información sobre la situación de conservación, endemismo y abundancia relativa. Los métodos intensivos de manejo forestal, como MDS, tienen una comunidad de mamíferos y aves más rica que el MMOBI y CS, pero similar a las de áreas bajo protección. La riqueza y abundancia de anfibios y reptiles, la mayoría en alguna categoría de riesgo, son bajas, especialmente en el MMOBI y CS, y casi ausentes en zonas de protección.

Abstract To determine the species composition, relative abundance and seasonal variation of different mosquitoes Genera (Aedes, Anopheles, Armigeres, Culex, and Culiseta) in different habitats the present research work was carried out in Entomology Research Laboratory of The University of Peshawar. Sampling performed from variety of permanent and temporary breeding habitats was carried out on monthly basis from targeted breeding sites for two consecutive years through dipping method. Species diversity in the survey sites was noted. Collection from these seventeen various types of potential larval habitats, yielded a total of 42,430 immature constituting 41,556 larvae and 874 pupae. Among these only 19,651 adult mosquitoes emerged comprising 11,512 female and 8,139 male mosquitoes. 78% (n= 15333) of mosquito larvae were from permanent and 22% (n=4318) were from temporary breeding sites. This study showed that Peshawar valley harbours 15 species from the genera Aedes, Anopheles, Armigeres, Culex and Culiseta. When the density of each species was examined, Culex quinquifasciatus was found to be dominant (79%) and constant in distribution. Among the temporary habitats Aedes albopictus was found as the most prevalent species particularly from tree holes and water cisterns. The highest intensity of mosquitoes was in June (2243 emerged adults) and November (2667 emerged adults) while the lowest was in January (203 emerged adults). A perfect positive correlation (r = +0.8) was found between temperature and population of mosquitoes (df 10 and α 0.05). The species diversity index for mosquitoes remained between 0.12 and 1.76. The Margalef’s richness components was noticeably low for bamboo traps (0.2) and fairly high for rice fields, Percolating water and Animal tracks (1.3) which shows the abundance of mosquito species in these habitats. Similarly Pielou’s Evenness was highest for bamboo traps (E=1) showing species uniform distribution. Animal tracks were presumed not only the diverse habitat rather also possessed high value for species richness and species evenness. Temperature, rainfall, humidity and other related attributes responsible for species variation and abundance need to be analysed further to pave way for controlling vector species in their oviposition targeted sites. composition (Aedes Anopheles Armigeres Culiseta method noted 42430 42 430 42,43 41556 41 556 41,55 87 pupae 19651 19 651 19,65 11512 11 512 11,51 8139 8 139 8,13 78 n= n (n 15333 22 n=4318 n4318 4318 (n=4318 1 examined 79% 79 (79% distribution cisterns 2243 (224 adults 2667 (266 203 (20 adults. . r +0.8 08 0 df 0.05. 005 0.05 05 0.05) 012 12 0.1 176 76 1.76 Margalefs Margalef s 0.2 02 2 (0.2 fields 1.3 13 3 (1.3 Pielous Pielou E=1 E1 E (E=1 evenness Temperature rainfall 4243 4 43 42,4 4155 55 41,5 1965 65 19,6 1151 51 11,5 813 8,1 7 1533 n=431 n431 431 (n=431 (79 224 (22 266 (26 20 (2 +0. 00 0.0 01 0. 17 1.7 (0. 1. (1. E= (E= 424 42, 415 5 41, 196 6 19, 115 11, 81 8, 153 n=43 n43 (n=43 (7 26 ( +0 (0 (1 (E n=4 n4 (n=4 +

Resumo Para determinar a composição de espécies, abundância relativa e variação sazonal de diferentes gêneros de mosquitos (Aedes, Anopheles, Armigeres, Culex e Culiseta) em diferentes habitats, o presente trabalho foi realizado no Laboratório de Pesquisa em Entomologia da Universidade de Peshawar. A amostragem coletada a partir de uma variedade de habitats de reprodução permanentes e temporários foi realizada mensalmente a partir de locais de reprodução alvo por 2 anos consecutivos através do método de imersão. A diversidade de espécies nos locais de pesquisa foi anotada. A coleta desses 17 tipos diferentes de habitats larvais potenciais rendeu um total de 42.430 imaturos, constituindo 41.556 larvas e 874 pupas. Destes, emergiram apenas 19.651 mosquitos adultos, sendo 11.512 fêmeas e 8.139 machos. 78% (n = 15333) das larvas do mosquito eram de criadouros permanentes e 22% (n = 4318) de criadouros temporários. Este estudo mostrou que o vale de Peshawar abriga 15 espécies dos gêneros Aedes, Anopheles, Armigeres, Culex e Culiseta. Quando a densidade de cada espécie foi examinada, Culex quinquifasciatus foi considerado dominante (79%) e constante na distribuição. Entre os habitats temporários, o Aedes albopictus foi encontrado como a espécie mais prevalente, principalmente em ocos de árvores e cisternas de água. A maior intensidade de mosquitos foi em junho (2.243 adultos emergidos) e novembro (2.667 adultos emergidos), enquanto a menor foi em janeiro (203 adultos emergidos). Uma correlação positiva perfeita (r = +0,8) foi encontrada entre temperatura e população de mosquitos (DF 10 e α 0,05). O índice de diversidade de espécies de mosquitos permaneceu entre 0,12 e 1,76. Os componentes de riqueza de Margalef foram visivelmente baixos para armadilhas de bambu (0,2) e razoavelmente altos para campos de arroz, água percolada e rastros de animais (1,3), o que mostra a abundância de espécies de mosquitos nesses habitats. Da mesma forma, a uniformidade de Pielou foi maior para armadilhas de bambu (E = 1), mostrando distribuição uniforme das espécies. As pegadas de animais foram presumidas não apenas como habitat diverso, mas também possuíam alto valor para riqueza e uniformidade de espécies. Temperatura, chuva, umidade e outros atributos relacionados responsáveis pela variação e abundância das espécies precisam ser analisados mais a fundo para abrir caminho para o controle de espécies de vetores em seus locais de oviposição alvo. (Aedes Anopheles Armigeres Culiseta imersão anotada 1 42430 42 430 42.43 imaturos 41556 41 556 41.55 87 pupas Destes 19651 19 651 19.65 11512 11 512 11.51 8139 8 139 8.13 machos 78 n 15333 22 4318 examinada 79% 79 (79% prevalente 2.243 2243 243 (2.24 emergidos 2.667 2667 667 (2.66 emergidos, , 203 (20 emergidos. . r +0,8 08 0 DF 0,05. 005 0,05 05 0,05) 012 12 0,1 176 76 1,76 0,2 02 (0,2 arroz 1,3, 13 1,3 3 (1,3) forma E 1, 1) diverso Temperatura chuva 4243 4 43 42.4 4155 55 41.5 1965 65 19.6 1151 51 11.5 813 8.1 7 1533 431 (79 2.24 224 24 (2.2 2.66 266 66 (2.6 20 (2 +0, 00 0,0 01 0, 1,7 (0, (1,3 424 42. 415 5 41. 196 6 19. 115 11. 81 8. 153 (7 2.2 (2. 2.6 26 ( +0 (0 (1, 2. + (1

Resumo Espécies de besouros Scolytinae, em altas populações, podem danificar a madeira e reduzir a produtividade de cultivos florestais. Esses besouros são monitorados com armadilhas iscadas com etanol e o aumento da eficiência das mesmas pode melhorar o manejo integrado desses insetos. O objetivo foi avaliar o aumento da eficiência de captura de Scolytinae com armadilha modelo semifunil, em dois experimentos, um incluindo elementos de madeira e outro aumentando a área de interceptação de voo e correlacionar o número desses besouros coletados com fatores climáticos. No experimento 1, ripas de Eucalyptus urophylla S. T. Blake foram fixadas, diretamente, no frasco coletor e em outro tratamento, além dessas ripas, fitas de Cedrela sp. foram inseridas no interior da mangueira porta isca. No experimento 2, a área de interceptação de insetos na armadilha, originalmente, com 480 cm2, foi ampliada para 1200 cm2 e comparada com a armadilha modelo Pet–Santa Maria com área de interceptação de 550 cm2. Coletas semanais foram realizadas entre maio de 2018 a junho de 2019. Os insetos capturados foram levados ao Laboratório de Biodeterioração da Madeira da Universidade Federal Rural do Rio de Janeiro (UFRRJ) onde foram triados, identificados em nível de família, contados e a abundância correlacionada com fatores climáticos. As análises estatísticas dos dados coletados foram processadas pelo programa BioStat® 5.3. No experimento 1 foram coletados 869 Scolytinae. Os números de besouros coletados por armadilha sem modificação, com ripas de E. urophylla e com ripas de E. urophylla + fita de Cedrela sp. foram semelhantes, 7,3 ± 3,8; 7,8 ± 6,2 e 7,7 ± 5,0 respectivamente. No experimento 2 foram coletados 4398 Scolytinae. O aumento da área de interceptação dos besouros aumentou a eficiência da armadilha semifunil, com 42,7 ± 20,5 Scolytinae coletados por armadilha comparado a semifunil original, 28,6 ± 12,6 e a Pet–Santa Maria, 20,4 ± 10,4. O número de Scolytinae não se correlacionou com os fatores climáticos no experimento 1 e se correlacionou com a temperatura, umidade relativa e velocidade do vento, mas não com a precipitação, no 2. A incorporação de ripas de E. urophylla ou fitas de Cedrela sp. na armadilha semifunil não aumentou o número de besouros coletados, mas, o aumento da área de intercepção de voo e a temperatura, umidade relativa do ar e velocidade do vento se correlacionaram com o número de besouros coletados.

Abstract Scolytinae species that, in high populations, can damage reducing wood production in forest crops. These beetles are monitored with traps baited with ethanol and increasing their efficiency can improve the integrated management of these insects. The objective was to evaluate the increase in the capture efficiency of Scolytinae with a semi-funnel trap model, in two experiments, one including wooden elements and other increasing the flight interception area and to correlate the numbers of these beetles collected with climatic factors. In the experiment 1, Eucalyptus urophylla S. T. Blake slats were directly attached to the collector flask and in another treatment, in addition to these slats, Cedrela sp. strips were inserted inside the bait holding hose. In the experiment 2, the insect interception area in the trap, originally 480 cm2, was expanded to 1,200 cm2 and compared with the model Pet–Santa Maria trap with an interception area of 550 cm2. Weekly collections were carried out between May 2018 and June 2019. The beetles collected were taken to the Wood Biodeterioration Laboratory of the Federal Rural University of Rio de Janeiro (UFRRJ) where they were sorted, identified at family level, counted and their number correlated with climatic factors. Statistical analyzes of the collected data were processed by the BioStat® 5.3 program. In the experiment 1 were collected 869 Scolytinae. The numbers of beetles collected per trap without modification, with E. urophylla slats and E. urophylla slats + Cedrela sp. strips were similar, 7.3 ± 3.8, 7.8 ± 6.2 and 7.7 ± 5.0 respectively. In the experiment 2 were collected 4,398 Scolytinae. Increasing the interception area of the beetles increased the efficiency of the semi-funnel trap, with 42.7 ± 20.5 Scolytinae collected compared to the original semi-funnel trap, 28.6 ± 12.6 and the Pet–Santa Maria, 20.4 ± 10.4, per trap. The number of Scolytinae did not correlate with climatic factors in the experiment 1 and it was correlated with temperature, relative humidity and wind speed, but not with precipitation, in the 2. The incorporation of E. urophylla slats or Cedrela sp. strips in the semi-funnel trap did not increase the number of beetles collected, but, the increase in the flight interception area and the temperature, relative humidity and wind speed were correlated with the number of beetles collected.

Abstract Epiphytes are considered indicators of forest ecological integrity, but the factors that explain their abundance are still not well understood. We here evaluated tree colonization by epiphytes in old-growth monospecific reforestation stands of Astronium urundeuva (M.Allemão) Engl. (Anacardiaceae) and Eucalyptus saligna Sm. (Myrtaceae), in comparison to a neighbor seasonal tropical forest fragment under similar environmental conditions. In each forest type, we identified and measured all trees (planted and colonizers) from 5-cm stem diameter in five 200 m² plots and quantified all vascular epiphytes per tree. Tree species were categorized by bark roughness, canopy deciduousness and growth rate. The abundance of epiphytes and the frequency of host trees were higher in the A. urundeuva plantation than in the native forest, with the E. saligna stand in an intermediate position. Also, we found that host traits influenced the abundance of epiphytes in their trunks. Host trees had average stem perimeter and height both higher than non-hosts, which indicates that colonization is more likely to occur in older trees. The average abundance of epiphytes per tree was higher in species with rough bark, but no relationship was found with canopy deciduousness or tree growth rate. We evidenced, therefore, that forest plantations, even if monospecific, can provide habitat for epiphytes. However, at community level, colonization success, either in native or restored forest, depends on the relative abundance of species whose bark type favors epiphytes establishment. integrity understood oldgrowth old M.Allemão MAllemão M Allemão (M.Allemão Engl Anacardiaceae (Anacardiaceae Sm Myrtaceae, Myrtaceae , (Myrtaceae) conditions planted colonizers 5cm cm 5 20 m roughness rate A E position Also trunks nonhosts, nonhosts non hosts, hosts non-hosts evidenced therefore plantations However level success establishment (Myrtaceae 2

Resumo Epífitas são consideradas indicadores de integridade ecológica em florestas, mas os fatores que explicam sua abundância ainda não são bem compreendidos. Neste estudo, avaliamos a colonização por epífitas em antigos talhões monoespecíficos de Astronium urundeuva (M.Allemão) Engl. (Anacardiaceae) e Eucalyptus saligna Sm. (Myrtaceae), em comparação com um fragmento vizinho de floresta estacional semidecidual sob condições ambientais semelhantes. Em cada tipologia florestal, identificamos e medimos todas as árvores (plantadas e que colonizaram os locais) a partir de 5 cm de diâmetro à altura padrão, em cinco parcelas de 200 m². Nelas, também quantificamos todas as epífitas vasculares por árvore. Em busca de uma explicação funcional para as diferenças entre espécies, utilizamos rugosidade da casca, deciduidade da copa e taxa de crescimento como atributos potencialmente relevantes. A abundância das epífitas e a frequência de forófitos foi maior no talhão de A. urundeuva do que na floresta nativa, com o talhão de E. saligna ocupando uma posição intermediária. Encontramos evidências, também, de que os atributos dos forófitos influenciaram a abundância de epífitas em seus troncos. Os forófitos apresentaram maior perímetro médio e altura que as árvores não hospedeiras, o que indica que a colonização é mais provável de ocorrer em árvores mais velhas. A abundância média de epífitas por árvore foi maior em espécies com casca rugosa, mas nem a deciduidade da copa, nem a velocidade de crescimento exerceram efeito neste aspecto. Evidenciamos, portanto que, plantações florestais, ainda que monoespecíficas, podem prover habitat para epífitas. Contudo, em nível de comunidade, o sucesso da colonização, seja em florestas nativas ou restauradas, depende da abundância relativa de espécies cujo tipo de casca favorece o estabelecimento de epífitas. compreendidos estudo M.Allemão MAllemão M Allemão (M.Allemão Engl Anacardiaceae (Anacardiaceae Sm Myrtaceae, Myrtaceae , (Myrtaceae) semelhantes florestal plantadas locais padrão 20 m² m Nelas relevantes nativa E intermediária evidências troncos hospedeiras velhas rugosa aspecto Evidenciamos florestais monoespecíficas Contudo comunidade restauradas (Myrtaceae 2

Resumen La cuenca del Usumacinta en el sureste de México es una de las de mayor riqueza ictiofaunística en el país, pero también una de las menos estudiadas. En el presente trabajo se clasificaron todas las especies registradas en la zona de influencia de la Reserva de la Biosfera Montes Azules de acuerdo con sus atributos funcionales y se analizaron las comunidades de peces de 3 sitios contrastantes. Se registraron 7 especies nativas con combinaciones de atributos únicas y 4 conjuntos de especies con alta similitud funcional. Se detectó la presencia de especies exóticas, pero únicamente en el cauce principal del río Lacantún. La abundancia relativa de las especies se distribuyó en todo el espectro funcional, excepto en el arroyo Danta, donde 2 entidades funcionales fueron las dominantes. El sitio Lacantún tuvo la mayor riqueza tanto taxonómica como funcional, pero con una diversidad intermedia debido a la distribución de las abundancias relativas. La clasificación sugerida en el presente estudio puede utilizarse para analizar otras series de datos para entender mejor el funcionamiento ecológico de las comunidades y las diferencias entre sitios sujetos a procesos de antropización en la región.

Abstract The Usumacinta River basin in southeastern Mexico has one of the country’s richest but least studied ichthyofaunas. This study presents a classification of functional traits for all fish species recorded in the area of influence of the Montes Azules Biosphere Reserve and a comparative analysis of the functional diversity among 3 contrasting sites. Seven native species, each with unique functional attribute combinations, and 4 sets of species with high functional similarity were identified. While exotic species were detected, they were found exclusively in the main channel of the Lacantún River. The relative abundance of species spanned the functional spectrum, except in the Danta stream, where 2 dominant functional entities were observed. The Lacantún site exhibited the highest taxonomic and functional richness, though with intermediate diversity attributed to the distribution of relative abundances. The functional classification proposed in this study can serve as a framework for analyzing other data series, facilitating a deeper understanding of ecological function across communities and for exploring variability among sites subject to differential anthropogenic impacts in the region.

RESUMO A cirurgia bariátrica induz uma variedade de alterações, incluindo alterações na composição da microbiota intestinal. A investigação avaliou o efeito da cirurgia bariátrica experimental relógio de areia com bypass gastrojejunal (EBSSC G-YB) na microbiota bacteriana fecal em porcos Landrace obesos. Foram utilizados 12 animais, com seis meses de idade, os quais foram suplementados com energia a partir de 2,5 meses, por um período de 120 dias, tempo suficiente para atingir a condição. Posteriormente, foi realizada intervenção com o EBSSC G-YB e avaliadas as alterações na microbiota, comparando-se um grupo experimental com dois grupos controle, em um período de 30 dias. O resultado foi que a microbiota dos três grupos apresentou Proteobacteria, Firmicutes e Bacteroidetes como as de maior abundância relativa, porém, ao final do experimento, destaca-se uma diferença, em que o filo Spirochaetes foi constante e com maior frequência no grupo experimental, enquanto para os grupos controle 1 e 2, foi o filo Tenericutes. Embora as diferenças não tenham sido significativas, qualitativamente houve maior diversidade no grupo experimental com maior abundância de Escherichia-Shigella e Pseudohongiella, o que pode ser útil como biomarcadores quantificáveis de perda de peso em pesquisas futuras. intestinal GYB G YB obesos animais idade 25 2 5 dias condição Posteriormente comparandose comparando se 3 Proteobacteria relativa porém experimento destacase destaca diferença Tenericutes significativas EscherichiaShigella Escherichia Shigella Pseudohongiella futuras

ABSTRACT Bariatric surgery induces a variety of changes, including changes in the composition of the gut microbiota. The investigation evaluated the effect of the experimental gastrojejunal bypass bariatric surgery hourglass (EBSSC G-YB) on the fecal bacterial microbiota in obese Landrace pigs. Twelve six-month-old animals were used, which were supplemented with energy from 2.5 months for a period of 120 days, enough time to reach the condition. Subsequently, they were intervened with the EBSSC G-YB and the changes in the microbiota were characterized, comparing an experimental group with two control groups in a period of 30 days. The result was that the microbiota of the three groups showed Proteobacteria, Firmicutes and Bacteroidetes as the ones with the highest relative abundance, however, at the end of the experiment a difference is highlighted, where the Phylum Spirochaetes was constant and more frequently in the experimental group, while for control groups 1 and 2 it was the Phylum Tenericutes. Although the differences were not significant, qualitatively there was greater diversity in the experimental group, with greater abundance of Escherichia-Shigella and Pseudohongiella, which may be useful as quantifiable biomarkers of weight loss in future research. GYB G YB pigs sixmonthold six month old used 25 5 2. 12 days condition Subsequently characterized 3 Proteobacteria however highlighted Tenericutes significant EscherichiaShigella Escherichia Shigella Pseudohongiella research

Abstract Review of an unpublished master’s dissertation by one of Brazil’s leading historians. Digital access to it, only recently made available, has revealed an innovative interpretation on one of Brazil’s economic history central questions: when, exactly, and why was there a crisis in coffee production at the Paraíba valley in the 19th century? The author relates the widely used slash and burn technique to factor endowments (land abundance and labor scarcity), thus presenting a new explanation to the valley’s and Brazil’s coffee production history, hitherto widely ignored. masters master s Brazils Brazil historians it available questions when exactly th century land scarcity, scarcity , scarcity) valleys ignored

Resumo Resenha da dissertação de mestrado de um dos mais importantes historiadores brasileiros, até hoje não publicada, cuja defesa completa 40 anos em 2023. A recente digitalização da obra revela uma interpretação inovadora sobre a decadência produtiva do Vale do Paraíba no fim do século XIX. O autor explica o sistema de plantio extensivo na região, com utilização de queimadas, como uma solução racional para o problema da oferta relativa de fatores à época: abundância de terras e escassez de trabalhadores. Cria, assim, uma terceira via interpretativa para a história do Vale e a crise do café na região, até hoje ignorada. brasileiros publicada 4 2023 XIX região queimadas época trabalhadores Cria assim ignorada 202 20 2

Abstract. Stock assessment of the striped marlin from the Mexican Pacific coast was done with historical (1963-2014) catch and effort data provided by commercial and recreational fleets, including bycatch data. Eight fishery-independent indices of relative abundance were used to fit a biomass dynamic model by maximum likelihood. For the most recent period (2015-2019), stock status was evaluated through simulations with the fitted model and available catch-per-unit-effort data of the recreational fleets as reference framework. Results indicated that up until 2014, striped marlin abundance showed 3 different trends. The first one was a decreasing trend from 1964 to 1977, where the catch and effort of commercial fleets exceeded the maximum sustainable yield (MSY) and the level of effort to reach MSY, respectively. The second one occurred from 1977 to 1990, and it was a stable trend with low abundance, less than the abundance when MSY is reached; this change was related to the reduction of commercial effort, although catches greater than the MSY were observed. The third one occurred from 1990 to 2014, and it showed increasing abundance due to the cessation of commercial fleets. This increase, however, has become slower because of the development of the recreational fishery and the increased bycatch. After 2014, the indicator estimated with the data from the recreational fleets suggested a shift in abundance; according to simulations, the fishing mortality was insufficient to explain this change. It is hypothesized that the natural mortality of striped marlin has been high since the mid-2010s. Conditions that could influence this situation and their implications on fishery management are discussed.

Resumen. Se evaluó el estado de explotación del marlin rayado en el Pacífico mexicano con datos históricos (1963-2014) de captura y esfuerzo de flotas comerciales y deportivas, incluso datos de captura incidental. Se estimaron 8 indicadores de abundancia relativa independientes para ajustar un modelo dinámico de biomasa por máxima verosimilitud. Para el periodo más reciente (2015-2019), se evaluó el estado de explotación a través de simulaciones hechas con el modelo ajustado, y se tomó como referencia los datos disponibles de captura por unidad de esfuerzo de las flotas deportivas. Los resultados indicaron que hasta 2014, la abundancia del marlin rayado ha pasado por 3 etapas diferentes. La primera fue una tendencia decreciente de 1964 a 1977, donde las capturas y el esfuerzo de las flotas comerciales excedieron, respectivamente, el máximo rendimiento sostenible (MRS) y el esfuerzo para alcanzar el MRS. La segunda ocurrió de 1977 a 1990, y fue una tendencia estable de baja abundancia, inferior a la abundancia cuando se alcanza el MRS; este cambio se relacionó con la reducción del esfuerzo de las flotas comerciales, aunque con capturas superiores al MRS. La tercera ocurrió de 1990 a 2014, y mostró una tendencia al incremento de la abundancia, debido al cese de operaciones de las flotas comerciales. Este incremento ha sido lento debido al desarrollo de la pesca deportiva y al incremento de las capturas incidentales. Después de 2014, el indicador para las flotas deportivas sugirió un cambio en la abundancia; de acuerdo con las simulaciones, la mortalidad por pesca fue insuficiente para explicar dicho cambio. Se plantea como hipótesis que la mortalidad natural del marlin rayado ha sido alta desde la década de 2010. Se discuten las condiciones que pudieron influir en esta situación y sus implicaciones en el manejo pesquero.

Summary: Maize is an important cereal in terms of human and animal nutrition worldwide. In our study, the bacterial microbiome composition of the soil rhizosphere was compared among three maize (Zea mays L.) races: forage (C1), dual-purpose (C2) and grain (C3). Additionally, the impact of the bacterial microbiome of the soil rhizosphere over yield was determined. Our study was conducted in plots located in the El Retiro Experimental Field at Universidad Autónoma Agraria Antonio Narro (UAAAN). Soil sampling was carried out in June 13, 2021, 51 days after sowing. DNA was extracted from three samples of each maize race. The V3-V4 region of the 16S rRNA gene was amplified and massive next-generation sequencing was performed with Illumina. The bioinformatic analysis was conducted in QIIME using the bacterial taxonomic reference of EzBioCloud. The phylum Proteobacteria, the class Phycisphaerae, the orders Shingomonadales, Micrococcales and Phycisphaerales, the families Moroxellaceae, Micrococcaceae, Chitinophagaceae, Shingobacteriaceae and Enterobacteriaceae, as well as the genera Acinetobacter, Shingobacterium and Cryseobacterium, were the bacterial taxa that differed in relative abundance among the three races (P < 0.05, Kruskal-Wallis test and an average dissimilarity > 0.10 in the SIMPER analysis). Among the taxa identified, plant growth promoting bacteria (PGPB) such as Acinetobacter and Sphingobacterium were detected. The race C2 showed the best yields of grain weight (15.56 Mg ha-1) and forage dry weight (10.61 Mg ha-1). Thus, we conclude that C2 represents a good option to preserve the seed and that further research should be developed for this race under different conditions.

Resumen: El maíz es un cereal de importancia en términos de nutrición humana y animal a nivel mundial. En el presente estudio se comparó el microbioma bacteriano de la rizosfera del suelo entre tres razas criollas de maíz (Zea mays L.), forrajero (C1), doble propósito (C2) y grano (C3); asimismo se determinó su impacto sobre el rendimiento. Se trabajó en parcelas ubicadas en el Campo Experimental El Retiro de la Universidad Autónoma Agraria Antonio Narro (UAAAN). El muestreo de suelo se efectuó el 13 de junio del 2021, 51 días después de la siembra. El DNA se extrajo a partir de tres muestras de cada tipo de raza de maíz. Se amplificó la región V3-V4 del gen 16S rRNA y se realizó secuenciación masiva de siguiente generación con Illumina; el análisis bioinformático se desarrolló en QIIME utilizando la referencia taxonómica bacteriana EzBioCloud. El phylum Proteobacteria, la clase Phycisphaerae, los órdenes Shingomonadales, Micrococcales y Phycisphaerales, las familias Moroxellaceae, Micrococcaceae, Chitinophagaceae, Shingobacteriaceae y Enterobacteriaceae, así como los géneros Acinetobacter, Shingobacterium y Cryseobacterium, fueron los taxa bacterianos que difirieron en abundancia relativa entre las tres razas (P < 0.05, prueba Kruskal-Wallis y una disimilitud promedio > 0.10 en el análisis SIMPER). Dentro de los taxa mencionados se detectaron bacterias promotoras de crecimiento vegetal (BPCV) como Acinetobacter y Sphingobacterium. Se concluye que, de las razas analizadas, la C2 mostró los mejores rendimientos de peso del grano (15.56 Mg ha-1) y peso seco en forraje (10.61 Mg ha-1), la cual representa una buena opción para preservar la semilla y realizar investigaciones posteriores bajo diferentes condiciones.

Resumen Introducción: Los ensilajes del pasto king grass (Cenchrus purpureus (Schumach.) Morrone, syn. Pennisetum purpuphoides) y cáscaras de piña (Ananas comosus) son alternativas de alimento para ganado en condiciones de escasez alimentaria. Objetivo: Describir las dinámicas de la microbiota presente en los ensilajes de king grass y piña durante el proceso de fermentación usando secuenciación de próxima generación (NGS) y evaluar el efecto de protección de Lacticaseibacillus paracasei_6714 como inoculante de ensilaje ante Listeria monocytogenes. Métodos: Usamos un diseño aleatorio no restringido para caracterizar la microbiota presente en ensilajes de king Grass cosechados 70 días después de rebrote y de cáscaras de piña. Inoculamos mezclas de pasto y cáscara con L. paracasei_6714 o L. monocytogenes, o ambos, con un tratamiento control sin inocular. El perfil nutricional y de fermentación fue evaluado luego de 30 días. Después de 15 y 30 días de fermentación, usamos un análisis de para determinar la dinámicas y diversidad de la microbiota en los ensilajes inoculados y control. Resultados: Los contenidos de materia seca y digestibilidad, no difirieron significativamente; sin embargo, hubo diferencias en proteína cruda, pH y ácidos orgánicos. Obtuvimos 4 432 secuencias variantes de amplicon de Proteobacteria, Firmicutes, Bacterioidetes, Actinobacteria, Verrucomicrobia, Planctomycetes y de Patescibacteria. La abundancia relativa de cada filo vario dependiendo del material y periodo de fermentación. Similitudes de filo fueron mayores al 70 % (pero no mayor que 50 % con Bray-Curtis a nivel de especie). Conclusión: Estas comunidades bacterianas parecen cumplir un papel importante durante la fermentación del ensilaje. Un manejo apropiado del proceso de ensilaje puede reducir o eliminar baterías patogénicas.

Abstract Introduction: King grass (Cenchrus purpureus (Schumach.) Morrone, syn. Pennisetum purpuphoides) and pineapple peel (Ananas comosus) silages are food alternatives for livestock in conditions of feed shortage. Objective: To describe the dynamics of the microbiota present in king grass and pineapple silage during the fermentation process using next generation sequencing (NGS) and to evaluate the protective effect of Lacticaseibacillus paracasei_6714 as a silage inoculum against Listeria monocytogenes. Methods: We used an unrestricted randomized design to characterize the microbiota present in silages made from king grass harvested 70 days after regrowth and pineapple peel. We inoculated mixtures of grass and peel with L. paracasei_6714 or L. monocytogenes, or both, with a non-inoculated treatment as control. The nutritional and fermentative profile was evaluated after 30 days. After 15 and 30 days of fermentation, we used 16S rRNA analysis to determine the dynamics and diversity of the microbiota in the inoculated and control silages. Result: Dry matter content and digestibility did not differ significantly; however, there were differences in crude protein, pH and organic acids. We obtained 4432 amplicon sequence variants of Proteobacteria, Firmicutes, Bacterioidetes, Actinobacteria, Verrucomicrobia, Planctomycetes and Patescibacteria. The relative abundance of each phylum varied depending on the material and fermentation period. Phylum similarity was over 70 % (but not greater than 50 % with Bray-Curtis at the species level). Conclusion: These bacterial communities seem to have an important role during silage fermentation. Proper management of silage processing can reduce or eliminate pathogenic bacteria.