The rapid advance of nanotechnology has led to the proliferation of a wide range of water-soluble nanomaterials and therefore, a profound impact on the environment, underscoring the need to devise effective methods for isolating and confining these nanomaterials. This study presents the development of a highly effective system capable of removing and storing gold nanoparticles in solution using modified resins with different linking groups, such as tris(2-aminoethyl) amine, 3-mercaptopropionic acid, 11-mercaptoundecanoic acid, and L-cysteine. The catalytic properties of the resins functionalized with the thiol group containing gold nanoparticles were evaluated, using the reduction of the nitro compounds (3,5-dinitrosalicylic acid, 4-nitroguaiacol and 4-nitroaniline) as model reaction. Solid-phase organic synthesis was employed to prepare the modified resins and their interaction with gold nanoparticles was characterized using infrared spectroscopy, thermogravimetric analysis, field emission scanning electron microscopy-energy dispersive spectroscopy, and X-ray photoelectron spectroscopy. The removal capacity of the resins was evaluated using ultraviolet-visible optical emission spectroscopy and inductively coupled plasma techniques. Ultraviolet-visible technique was suitable to assess the catalytic properties of the functionalized resins containing gold nanoparticles. It was determined that the functionalized resins showed remarkable capacity for complex gold nanoparticles, removing them from the solution. The most significant removal was with 11-mercaptoundecanoic acid. watersoluble water soluble therefore environment groups tris2aminoethyl trisaminoethyl tris 2 aminoethyl tris(2-aminoethyl amine 3mercaptopropionic mercaptopropionic 3 acid 11mercaptoundecanoic mercaptoundecanoic 11 Lcysteine. Lcysteine L cysteine. cysteine L-cysteine 3,5dinitrosalicylic 35dinitrosalicylic dinitrosalicylic 3,5 5 4nitroguaiacol nitroguaiacol 4 4nitroaniline nitroaniline 4-nitroaniline reaction Solidphase Solid phase analysis microscopyenergy microscopy energy Xray X ray ultravioletvisible ultraviolet visible techniques Ultravioletvisible Ultraviolet 1 5dinitrosalicylic 35 3,

Niobium pentoxide shows an interesting reactivity that allows the control of different aspects of its morphology and chemistry. In this study, Nb2O5 nanoparticles were modified with protoporphyrin IX (PPIX) and tris(ethynylphenyl) pyrene derivative (PyPh3) by using 3-aminopropyltriethoxysilane as linkage group and used as photosensitizers against lung cancer. The antitumor photoactivity against the A549 tumor cell line as a model of in vitro study showed half maximal inhibitory concentration (IC50) ca. 15 μmol L-1 for both materials and the absence of dark activity, indicating the viability of dye-modified Nb2O5 as a photodynamic therapy (PDT) agent. chemistry NbO Nb O Nb2O PPIX (PPIX trisethynylphenyl tris ethynylphenyl tris(ethynylphenyl PyPh3 PyPh (PyPh3 3aminopropyltriethoxysilane aminopropyltriethoxysilane 3 cancer A A54 IC50 IC (IC50 ca 1 L1 L L- activity dyemodified dye PDT (PDT agent (PyPh A5 IC5 (IC5 (IC

Abstract The effects of antibiotics on sperm longevity in collared peccary (Pecari tajacu) fresh diluted semen was evaluated. Semen samples from six adult males were collected by electroejaculation and diluted in Tris-citrate-fructose alone (control) and plus streptomycin-penicillin (2 mg/ml-2000 IU/ml) or gentamicin (70 µg/ml). Membrane integrity and functionality, mitochondrial activity and sperm morphology were assessed subjectively. Sperm motility and other kinetic parameters were objectively assessed using CASA (computer-assisted semen analysis). The semen diluted according to the treatments were submitted to the thermoresistance test, incubated at 37 ° C, and the sperm parameters analyzed at 0, 30, 60, 120 and 180 min. The average values of the treatments were compared with each other and between the times. There were no differences (P > 0.05) between treatments until the end of the test. Control and streptomycin-penicillin samples maintained sperm function for up to 180 min (with total motility of 24.3 ± 7.1% and 28 ± 8.7%, respectively). Gentamicin aliquots retained most parameters until the end of the incubation, except for membrane integrity and mitochondrial activity that declined (P < 0.05) at 180 min (53.1 ± 7.1% and 50.7 ± 6.2%, respectively) compared to 0 min (80.5 ± 4.7% and 86.3 ± 3.4%, respectively). In conclusion, a multiparametric thermoresistance test proved that Tris-based extenders used for collared peccary semen can be effectively supplemented by streptomycin-penicillin (2 mg/ml-2000 IU/ml) or gentamicin (70 µg/ml), especially during 180-min incubation at 37 °C. Pecari tajacu evaluated Triscitratefructose Tris citrate fructose control (control streptomycinpenicillin streptomycin penicillin 2 ( mg/ml2000 mgml2000 mgml mg/ml 2000 mg ml mg/ml-200 IU/ml IUml IU 70 (7 µg/ml. µgml µg/ml . µg µg/ml) functionality subjectively computerassisted computer assisted analysis. analysis analysis) 3 C 30 60 12 18 times P 0.05 005 05 243 24 24. 71 7 1 7.1 87 8 8.7% respectively. respectively 53.1 531 53 (53. 507 50 50. 62 6 6.2% 80.5 805 80 5 (80. 47 4 4.7 863 86 86. 34 3.4% conclusion Trisbased based µg/ml, , 180min °C ml2000 mg/ml200 mgml200 200 mg/ml-20 0.0 00 7. 8.7 53. (53 6.2 80. (80 4. 3.4 ml200 mg/ml20 mgml20 20 mg/ml-2 0. 8. (5 6. (8 3. ml20 mg/ml2 mgml2 mg/ml- ml2

Reactive oxygen species at supra-physiological levels trigger oxidative stress during cryopreservation, which can be neutralised by incorporating suitable antioxidants into the semen extender medium. This study was intended to explore the effect of asiatic acid (AA) as an antioxidant in semen extender on frozen-thawed sperm quality and in vivo fertility of bull sperm. Semen was collected from Holstein Friesian bulls for 10 consecutive weeks (total ejaculates = 60). Semen was cryopreserved with a Tris citric acid egg yolk-based extender supplemented with 0 (control), 20, 40, 60, and 100 μM AA. The supplementation of the extender with 40 and 60 μM AA improved (p < 0.05) post-thaw motility kinematics, plasma membrane integrity, acrosome integrity, sperm viability, and DNA integrity of bull sperm. Mitochondrial membrane potential was high (p < 0.05) with 60 μM of AA concentration in extender media. The catalase activity in seminal plasma was maintained (p < 0.05) when semen was added with 20, 40, and 60 μM of AA. The in vivo fertility was found to be significantly high with the semen extended with 60 μM AA. Conclusively, this study showed that AA supplementation in semen extender significantly improved sperm motility kinematics and cell integrity, conserved antioxidant enzyme activity, and improved in vivo fertility.

Chloroquine is a drug belonging to the aminoquinoline family that is widely used for treating diseases such as lupus, cancer, and malaria. In this sense, the present work describes the development of a simple method based on electrogenerated chemiluminescence (ECL) and a screen-printed carbon electrode (SPE) modified with carbon black for chloroquine determination. The carbon black modified SPE was characterized by scanning electron microscopy, Raman spectroscopy, cyclic voltammetry and linear sweep voltammetry to obtain the ECL-potential curves. The ECL method is based on the chemiluminescence resulting from the interaction between chloroquine and the tris(2’2-bipyridyl) ruthenium(II) complex. Under optimized experimental conditions, the method showed a wide linear working range between 0.5 and 500 µmol L-1. The method presented good precision and accuracy in drug samples used in the treatment of malaria and artificial urine sample, showing recovery values from 100 to 103% and 99 to 103%, respectively. lupus cancer sense (ECL screenprinted screen printed (SPE determination microscopy spectroscopy ECLpotential potential curves tris22bipyridyl trisbipyridyl tris 2 bipyridyl tris(2’2-bipyridyl rutheniumII ruthenium II ruthenium(II complex conditions 05 0 5 0. 50 L1. L1 L 1. 1 L-1 sample 10 103 9 respectively L-

Abstract: The bactericidal permeability increasing protein is a molecule of the immune system which participates in the defense against pathogens. This protein binds to Gram-negative bacterial membranes altering permeability and inducing lysis. It also binds to free lipopolysaccharides, inhibiting Theo sígnala chat leads no inflamarían. In admitían, tris porten induces he opsonization activity contributing to phagocytosis. The role of the bactericidal permeability increasing protein, during infections by Gram-positive bacteria is still controversial. However, this protein increases in meningitis caused by Streptococcus pneumoniae and Neisseria meningitidis and during respiratory infections caused by the influenza A virus, possibly modulating the production of proinflammatory cytokines. In other respiratory disorders such as chronic obstructive pulmonary disease, cystic fibrosis, and asthma, the production of autoantibodies against this protein is recurrent. This antibody production reduces the bactericidal permeability increasing protein serum levels, decreasing antimicrobial defense against pulmonary infections. Therefore, it is essential to understand the role of this protein during respiratory diseases to propose possible therapies to improve patient health.

Resumen: La proteína bactericida que aumenta la permeabilidad es una proteína del sistema inmune que participa en la defensa contra patógenos. Esta proteína puede unirse a las membranas de bacterias Gram negativas, alterando la permeabilidad e induciendo la lisis. También se une a los lipopolisacáridos libres al inhibir la señalización que lleva a la inflamación. Además, esta proteína induce opsonización, contribuyendo a la fagocitosis. El papel de la proteína bactericida que aumenta la permeabilidad, durante las infecciones por bacterias Gram positivas aún es controversial, pero se ha demostrado su inducción durante la meningitis ocasionada por Streptococcus pneumoniae y Neisseria meningitidis y durante infecciones respiratorias generadas por el virus de la influenza A, posiblemente modula la producción de citocinas proinflamatorias. En otros padecimientos respiratorios como: la enfermedad pulmonar obstructiva crónica, la fibrosis quística y el asma, se producen por lo común autoanticuerpos contra esta proteína, disminuyendo sus niveles séricos, lo que repercute en la defensa contra infecciones pulmonares. Por ello, es importante comprender el papel de la proteína bactericida que aumenta la permeabilidad durante las enfermedades respiratorias, con el fin de proponer posibles terapias para mejorar la salud de los pacientes.

Abstract The cryopreservation reduces ram sperm quality, decreasing the pregnancy rate of ewes inseminated with thawed sperm. Hence, we aimed to improve the post-thaw quality of ram sperm replacing egg yolk on Tris-Glucose extender with different concentrations of LDL (2 or 8%), associated with the addition of 10 mM non-enzymatic antioxidants (ascorbic acid, hydroxytoluene butylate, ascorbyl palmitate, and trehalose). Semen samples were collected from six rams, split into different treatments, and frozen. After thawing, kinematic (CASA), structural (propidium iodide and carboxyfluorescein diacetate) and functional (hypoosmotic test) sperm membrane integrity was assessed. Total motility, VCL, and LIN were also assessed in thawed samples during 3 h of incubation (38 °C). The results showed that hydroxytoluene butylate at 10 mM in Tris-Glucose extender with 8% LDL improved velocity parameters immediately post-thaw compared with Tris-Glucose egg yolk extender, as well as prevented the reduction of total motility and VCL after incubation. There was no benefit of adding ascorbic acid and trehalose. Moreover, for the first time, it was shown the motility impairment promoted by ascorbyl palmitate to ram sperm. Hence postthaw post thaw TrisGlucose Tris Glucose 2 ( 8%, 8 , 8%) 1 nonenzymatic non enzymatic trehalose . trehalose) rams treatments frozen thawing CASA, CASA (CASA) propidium diacetate hypoosmotic test 38 (3 °C. C °C °C) Moreover time (CASA

Abstract Objectives The authors evaluated mortality and indices of cost of care among inpatients with Atrial Fibrillation (AF) and a diagnosis of a Temperature-Related Illness (TRI). The authors also assessed trends in the prevalence of TRIs among AF hospitalizations. Methods In this cross-sectional study, the authors used discharge data from the Nationwide Inpatient Sample (NIS) collected between January 2005 and September 2015 to identify patients with a diagnosis of AF and TRI. Outcomes of interest included in-hospital mortality, invasive mechanical ventilation, hospital length of stay, and cost of hospitalization. Results A total of 37,933 encounters were included. The median age was 79 years. Males were slightly overrepresented relative to females (54.2% vs. 45.8%, respectively). Although Blacks were only 6.6% of the cohort, they represented 12.2% of the TRI cases. Compared to non-TRI-related hospitalizations, a diagnosis of a TRI was associated with an increased likelihood of invasive mechanical ventilation (16.5% vs. 4.1%, p< 0.001), longer length-of-stay (5 vs. 4 days, p <0.001), higher cost of care (10,082 vs. 8,607, in US dollars p <0.001), and increased mortality (18.6% vs. 5.1%, p <0.001). Compared to non-TRI, cold-related illness portends higher odds of mortality 4.68, 95% Confidence Interval (4.35-5.04), p <0.001, and heat-related illness was associated with less odds of mortality, but this was not statistically significant 0.77 (0.57-1.03), p= 0.88. Conclusion The occurrence of TRI among hospitalized AF patients is small but there is an increasing trend in the prevalence, which more than doubled over the decade in this study. Individuals with AF who are admitted with a TRI face significantly poorer outcomes than those admitted without a TRI including higher mortality. Cold-related illness is associated with higher odds of mortality. Further research is required to elucidate the pathogenic mechanisms underlying these findings and identify strategies to prevent TRIs in AF patients. (AF TemperatureRelated Temperature Related . (TRI) hospitalizations crosssectional cross sectional study NIS (NIS 200 201 inhospital stay hospitalization 37933 37 933 37,93 7 years 54.2% 542 54 2 (54.2 vs 458 45 8 45.8% respectively. respectively respectively) 66 6 6.6 cohort 122 12 12.2 cases nonTRIrelated non related 16.5% 165 16 5 (16.5 41 1 4.1% 0.001, 0001 0.001 , 0 001 0.001) lengthofstay ( days <0.001 <0.001) 10,082 10082 10 082 (10,08 8607 607 8,607 18.6% 186 18 (18.6 51 5.1% <0.001. nonTRI, nonTRI TRI, non-TRI coldrelated cold 468 68 4.68 95 4.355.04, 435504 4.35 5.04 35 04 (4.35-5.04) heatrelated heat 077 77 0.7 0.571.03, 057103 0.57 1.03 57 03 (0.57-1.03) 088 88 0.88 Coldrelated Cold (TRI 20 3793 3 93 37,9 54.2 (54. 45.8 6. 12. 16.5 (16. 4.1 000 0.00 00 <0.00 10,08 1008 08 (10,0 860 60 8,60 18.6 (18. 5.1 46 4.6 9 355 4.355.04 43550 435 4.3 504 5.0 (4.35-5.04 07 0. 571 0.571.03 05710 057 0.5 103 1.0 (0.57-1.03 0.8 379 37, 54. (54 45. 16. (16 4. 0.0 <0.0 10,0 100 (10, 86 8,6 18. (18 5. 4.355.0 4355 43 50 (4.35-5.0 0.571.0 0571 05 1. (0.57-1.0 (1 <0. 10, (10 8, 4.355. (4.35-5. 0.571. (0.57-1. <0 4.355 (4.35-5 0.571 (0.57-1 < (4.35- (0.57- (4.35 (0.57 (4.3 (0.5 (4. (0. (4 (0

Abstract The aim of the present study was to evaluate semen cryopreservation with ACP-Lact® diluent, which consists of coconut water powder (ACP) added to goat milk powder. After thawing, the samples were evaluated for sperm kinetics, membrane evaluation and in vivo insemination. For cryopreservation, a pool was made with the ejaculate of six goats, diluted in four equal aliquots for the respective treatments: T1 (ACP-Lact®); T2 (ACP-Lact® 50%); T3 (ACP + 2.5% egg yolk) and T4 (Tris + 2.5% egg yolk). After dilution of the treatments, the samples were placed in 0.5 ml straws and chilled at a rate of -1.07°C/min. After reaching 4°C and stabilizing for one hour, the straws were placed in nitrogen vapour at -60°C for 15 minutes and then immersed in liquid nitrogen (-196ºC). The straws were thawed in a 37°C water bath and kinetic assessments were performed immediately using a computerized semen analysis program (CSA), viability (EN), membrane functionality (HOST), mitochondrial activity (DAB) and DNA integrity assessment of spermatozoa. For the in vivo experiment, ten goats were inseminated, divided into two groups of five goats each, G1 inseminated with ACP-Lact® and G2 with ACP, by fixed-time artificial insemination (FTAI). Regarding the kinetic parameters, the ACP-Lact® treatment showed higher progressive motility (PM) and sperm velocity than the other treatments (36.77%). In the VSL parameter the ACP-Lact diluent was superior to ACP and Tris. In viability the treatment with ACP-Lact® was superior to the treatment with Tris, 95% and 83% respectively. In FTAI two goats were born out of the 5 goats inseminated with ACP-Lact®. It was concluded that the use of ACP-Lact® for cryopreservation of caprine semen is efficient in maintaining seminal parameters during thawing in vitro and in vivo and proved to be a good alternative extender for the caprine species. ACPLact® ACPLact Lact® Lact kinetics T (ACP-Lact®) (ACP-Lact 50% 50 50%) 25 2 2.5 yolk Tris yolk. . 05 0 0. 1.07°C/min. 107Cmin Cmin 1 07 C min -1.07°C/min 4C 4 hour 60°C 60C 60 196ºC. 196ºC ºC (-196ºC) 37C 37 CSA, CSA , (CSA) EN, EN (EN) HOST, HOST (HOST) DAB (DAB spermatozoa experiment each G fixedtime fixed time FTAI. (FTAI) PM (PM 36.77%. 3677 36.77% 36 77 (36.77%) 95 83 respectively ACPLact®. Lact®. species 2. 1.07°C/min 6 (-196ºC 3 (CSA (EN (HOST (FTAI 367 36.77 7 (36.77% 9 8 36.7 (36.77 36. (36.7 (36. (36 (3 (

Abstract The study evaluated the effect of two animal protein-free extenders on ram semen cryopreservation of two tropical hair breeds. The ejaculates were collected from 8 rams (4 Blackbelly and 4 Pelibuey), which were mixed (pooled) by breed, diluted and cryopreserved in three different extenders: Animal protein [Tris egg yolk (Triladyl®)], and animal protein-free extenders AndroMed® (lecithin soy bean) and OPTIXcell® (liposome). Sperm analyses of total (TM) and progressive (PM) motility, viability, mitochondrial activity, acrosome integrity, and plasma membrane integrity (PMI) were carried out at 0 and 6 h after semen thawing. OPTIXcell® and Triladyl® extenders showed similar results between them and differed with AndroMed® in TM, PM, viability, and PMI (P < 0.0017). In the Blackbelly breed, the TM was higher (P < 0.0159) in OPTIXcell® than in AndroMed®. In the Pelibuey breed, the OPTIXcell® and Triladyl® showed similar results between them and differed with AndroMed® in TM, PM, viability, mitochondrial activity, and PMI (P < 0.0140). However, Triladyl® showed a higher percentage of sperm with intact acrosome than AndroMed® (P < 0.0392). In both breeds, spermatic parameters decreased progressively over the incubation time similarly in all three extenders. In conclusion, OPTIXcell® and Triladyl® proved to be the best extenders to cryopreserve Blackbelly and Pelibuey ram semen. However, OPTIXcell® is an animal protein-free extender that decreases the risk of bacterial contamination, whereas Triladyl® is composed of animal protein (egg yolk), which may impact the fertilizing capacity of sperm.

Resumen El desarrollo teórico y práctico del fenómeno de acidificación oceánica (AO) involucra al océano abierto y si lo poco que se sabe del significado de la variación del pH y el sistema de carbonatos en ambientes costeros tiene algún efecto en la AO. Las mediciones potenciométricas tradicionales de pH se hacen en la escala NBS (pHNBS), desarrollada para aguas dulces, pero para la AO es necesario usar la escala total (pHT), que incluye el efecto de los iones hidronio y los complejos formados con el ion sulfato. En la laguna costera artificial La Escollera en Santa Marta (Colombia) se probó una metodología para calcular p H T usando series de mediciones in-situ de potencial (EX) realizadas con un electrodo de pHNBS. Para ello se usó la expresión , la cual calcula el pHT(X) de la muestra a partir del the pHT(TRIS) de la solución estándar TRIS, los potenciales E medidos a la temperatura T, y las constantes R y F. ETRIS se determinó experimentalmente para el rango de temperaturas de la laguna y la regresión lineal presentó un coeficiente de determinación (R2) de 0,9977. En un primer análisis cualitativo se constató que las variaciones de pH durante el ciclo día-noche están estrechamente asociadas con las del oxígeno disuelto, por la producción y consumo de CO2 por fotosíntesis y respiración. Estas variaciones de alta frecuencia son de mayor magnitud que las del océano abierto, generando preguntas sobre el efecto real de la AO en los ecosistemas costeros.

Abstract Most of the theoretical and practical development of the ocean acidification (OA) phenomenon involves the open ocean, and not much is known of the significance of variation in pH and carbonate system in coastal environments and the effect, if any, of OA. Traditional potentiometric pH measurements are carried out on the NBS scale (pHNBS), developed for freshwaters, but for OA it is necessary to use the total scale (pHT), which includes the additional ions of seawater. Using a series of in-situ measurements of potential, carried out with a pHNBS electrode in the artificial coastal lagoon La Escollera in Santa Marta (Colombia), a methodology to calculate p H T was tested. For this, the equation was used, which calculates pHT(X) of the sample from the pHT(TRIS) of the TRIS standard solution, the potentials E measured at temperature T, and the constants R and F. ETRIS was determined experimentally for the lagoon temperature range, and the linear regression showed a coefficient of determination (R2) of 0.9977. In a first qualitative analysis, it was verified that pH variations during the day-night cycle are closely associated with those of oxygen, from the production and consumption of CO2 by photosynthesis and respiration. These high-frequency variations are of greater magnitude than those of the open ocean, raising questions about the real effect of OA on coastal ecosystems.

Abstract Effects were assessed of the dilutants TRIS and ACP - 101c® with the addition of different guinea fowl (Numida meleagris) egg yolk concentrations. Fifteen ejaculates were collected from five goats of the Anglo Nubian breed. The ejaculates were pooled and then divided into 12 groups, two control groups (GC1 TRIS, with 2.5% Gallus gallus domesticus hen egg yolk GOGD), (GC2 Control Group ACP - 101c®, with the addition of 2.5% Gallus gallus domesticus hen egg yolk GOGD) and ten experimental groups (EG), containing TRIS and ACP added with different concentrations of egg yolk from guinea hen (Numida meleagris) (TRIS 2,5% GONM; TRIS 5% GONM; TRIS 10% GONM; TRIS 15% GONM; TRIS 20% GONM; ACP® 2,5% GONM; ACP® 5% GONM; ACP® 10% GONM; ACP® 15% GONM; ACP® 20% GONM). Then cryopreservation was carried out and the samples stored in liquid nitrogen (-196 °C). After seven days, the samples were thawed and assessed for spermatic kinetics, immunofluorescence and sperm morphology. Analysis of GOMN by the CASA system showed that the various parameters were similar to those of GOGD (P>0.05). The membrane integrity, mitochondrial potential and the acrosome were not influenced by the treatment (P>0.05) nor by the dilutant used for cryopreservation (P>0.05). The spermatic morphology was also preserved by the different GOGD and GONM concentrations in the ACP® and TRIS dilutants, with no statistically significant differences (P<0.05). It was concluded that Numida meleagris egg yolk, as external membrane cryoproctant added to the dilutants ACP-101c® and TRIS, improved goat semen quality.

RESUMO: Este estudo versa sobre os processos de produção e circulação de um manual intitulado Ginástica doméstica, médica e higiênica ou representação e descrição de movimentos ginásticos, publicado pela primeira vez em Leipzig, no ano de 1855, pelo médico alemão Daniel Gottlob Moritz Schreber e endereçado a pais e educadores. Partindo de uma escala particular de observação que inclui singularidades que caracterizam a difusão do referido manual entre a segunda metade do século XIX e início do XX, buscou-se identificar as estratégias autorais e editoriais adotadas, bem como os exercícios de “fabricação” presentes nas várias traduções, de modo a compreender como os textos impressos conferem e agregam efeitos de verdades a determinados saberes sobre o corpo. De modo mais específico, são analisadas duas traduções da obra que circularam no Brasil. A primeira publicada em Lisboa, em 1879, e a segunda no Rio de Janeiro pelo jornal Gazeta de Notícias, em 1887. O estudo nos permite afirmar que foram as traduções, como estratégias de apropriação e circulação cultural, que permitiram a esta obra um notável alcance em diferentes países.

ABSTRACT: This study deals with the production and circulation processes of a manual entitled Ginástica doméstica, médica e higiênica ou representação e descrição de movimentos ginásticos, first published in Leipzig, in 1855, by the German physician Daniel Gottlob Moritz Schreber and addressed to parents and educators. Starting from a particular scale of observation that includes singularities that characterize the diffusion of the referred manual between the second half of the 19th century and the beginning of the 20th, we sought to identify the adopted authorship and editorial strategies, as well as the “manufacturing” exercises present in the various translations, in order to understand how printed texts confer and add effects of truths to certain knowledge about the body. More specifically, two translations of the work that circulated in Brazil are analyzed. The first was published in Lisbon, in 1879, and the second in Rio de Janeiro by the newspaper Gazeta de Notícias, in 1887. The study allows us to affirm that it was translations, as a strategy of appropriation and cultural circulation, that allowed tris work to achieve a remarkable achievement reach in different countries.

RESUMEN: Este estudio aborda los procesos de producción y circulación de un manual titulado Ginástica doméstica, médica e higiênica ou representação e descrição de movimentos ginásticos, publicado por primera vez en Leipzig, en 1855, por el médico alemán Daniel Gottlob Moritz Schreber y dirigido a padres y educadores. Partiendo de una escala de observación particular que incluye singularidades que caracterizan la difusión del referido manual entre la segunda mitad del siglo XIX y principios del XX, se buscó identificar la autoría adoptada y las estrategias editoriales, así como los ejercicios de “fabricación” presentes en las distintas traducciones, para comprender cómo los textos impresos confieren y agregan efectos de verdades a ciertos conocimientos sobre el cuerpo. Más específicamente, se analizan dos traducciones del trabajo que circuló en Brasil. La primera fue publicada en Lisboa, en 1879, y la segunda en Río de Janeiro por el diario Gazeta de Notícias, en 1887. El estudio permite afirmar que fueron las traducciones, como estrategias de apropiación y circulación cultural, las que permitieron a este manual un alcance notable en diferentes países.

In this work, we have studied both experimentally and theoretically the oxybutynin chiral discrimination. According to the main results, the enantioseparation of oxybutynin was efficiently achieved by high performance liquid chromatography in normal phase using the Chiralpak® AD column, which has amylose tris (3,5‐dimethylphenylcarbamate) as a chiral selector. The energetic and structural parameters obtained via density functional theory calculations pointed out the chiral discrimination as well as the enantiomeric elution order of oxybutynin, thus explaining, at molecular level, the experimental data. Finally, the strength of the hydrogen bonds played a key role in the discrimination between the oxybutynin diastereomeric complexes formed.

Abstract Kacang goats are small ruminants produced by low-income households in smallholder and farm to reduce poverty and prevent undernutrition. Studies to find a cryopreservation protocol for Kacang goat semen are expected to multiplication of genetically superior animals selected by the paternal lineage. This study evaluated the effect of thawing temperature and supplementation of the green tea extract nanoparticle in skim milk-egg yolk (SM-EY) extender on post-thaw sperm quality of Kacang goat semen. Six ejaculates of Kacang goat were diluted in SM-EY supplemented or not (control group) with 0.001 mg/mL NPs GTE. The diluted semen was packaged with 0.25 mL straws (insemination dose: 60x106 sptz/mL) and cryopreserved. Then, six samples of the control group and NPs GTE groups were thawed at 37°C or 39°C sterile water for 30 s and submitted to sperm quality evaluations. The sperm viability, motility, and intact of the plasma membrane (IPM) were higher (p<0.05) in NPs GTE group than control group. In contrast, the NPs GTE group presented lower (p<0.05) malondialdehyde levels and sperm DNA fragmentation (SDF) compared with the control group. The catalase levels were not significantly different (p > 0.05) between the control and NPs GTE groups. Thawing at 39°C resulted in higher (p<0.05) sperm viability, motility, and IPM than thawing at 37°C. However, thawing at 39°C group presented lower (p<0.05) malondialdehyde levels compared with thawing at 37°C. SDF and catalase levels were similar (p>0.05) between thawing at 37°C and thawing at 37°C. In conclusion, supplementation of 0.001 mg/mL of NPs GTE in SM-EY extender and thawing temperature of 39°C resulted in a better quality of frozen-thawed Kacang goat semen. lowincome low income undernutrition lineage milkegg milk egg SMEY SM EY (SM-EY postthaw post thaw 0001 0 001 0.00 mgmL mg 025 25 0.2 insemination dose x 60x10 sptz/mL sptzmL sptz cryopreserved Then 37C C 37 39C 39 3 evaluations viability motility (IPM p<0.05 p005 p 05 (p<0.05 contrast (SDF 0.05 005 However p>0.05 (p>0.05 conclusion frozenthawed frozen 000 00 0.0 02 2 0. 60x1 p<0.0 p00 (p<0.0 p>0.0 (p>0.0 60x p<0. p0 (p<0. p>0. (p>0. p<0 (p<0 p>0 (p>0 p< (p< p> (p>