Abstract Lichens are symbiotic organisms, usually composed of a fungal partner, the mycobiont, and one or more photosynthetic partners, the photobiont, which is most often either a green alga or a cyanobacterium, that harbor a diverse community of invertebrates such as rotifers, tardigrades, mites, springtails, crustaceans, and nematodes. In this work, we isolated the nematode Geraldius galapagoensis (Chambersiellidae) associated with the lichen Hyperphyscia syncolla (Physciaceae) in a region of Buenos Aires Province, Argentina. This species was discovered in a tropical forest of Ecuador and is characterized mainly by a head offset by a constriction from the rest of the body, a esophagus with a cylindrical pharyngeal corpus without a median bulb, a narrow isthmus and an oval basal pharyngeal bulb with strong transverse/butterfly valve apparatus, a tail curved ventrally, ending in dorsally hooked end; the male with seven pairs of latero-ventral pre-anal papillae and three pairs of post-anal in the following positions: one pair latero-ventral and two pairs dorso-lateral and two slightly curved spicules with asymmetric manubrium with an anterior extension. The comparison of the morphometrics of our G. galapagoensis male with that of the original description shows that the body length is shorter, as are the distance of the excretory pore to the anterior end and the tail. On the other hand, the distance from the anterior end to the nerve ring and the esophagus length are greater. The head width, body diameter and spicule length are quite similar. We provide a morphological and morphometrical characterization of a G. galapagoensis second isolate and the first world report of molecular sequences belonging to this species. organisms partner mycobiont partners photobiont cyanobacterium rotifers tardigrades mites springtails crustaceans nematodes work Chambersiellidae (Chambersiellidae Physciaceae (Physciaceae Province Argentina transversebutterfly transverse butterfly apparatus ventrally lateroventral latero ventral preanal pre anal postanal post positions dorsolateral dorso lateral extension G shorter hand greater width similar

Abstract Communication in science requires standardized terminology with concepts unified that facilitate the processing and exploration of information in any knowledge domain. The morphology is not the exception; however, it has challenged problems, called “the linguistic problem of the morphology”, which is related to the processing of morphological data result of taxonomic work. Ontologies, standardized vocabularies expressed through language parsable (Resource Description Framework, RDF) by machines, are proposed to resolve the linguistic problems in morphology. Springtails are dominants in the soil and other types of habitats with ca 9000 described species. The anatomical terms employed in Collembola are not exempt from problems such as the presence of homonyms related to the chaetotaxy, idiosyncratic language employed in morphological descriptions, and lack of consensual definitions of anatomical terms, which difficult the comparison of morphological data. Collembola Anatomy Ontology (CLAO) is built according to principles established by The OBO Foundry and includes 1554 anatomical terms for the region of the cuticle, appendages, anatomical systems, and cells. CLAO is directed toward morphological descriptions and the production of phenotypic data produced from taxonomic and anatomical works before the obtaining of homologies in a phylogenetic framework. Also, to increase the interoperability with other anatomical ontologies for Arthropoda and knowledge domains. domain exception however morphology, , morphology” work Ontologies Resource Framework RDF machines 900 species chaetotaxy (CLAO 155 cuticle appendages systems cells framework Also domains 90 15 9 1

Resumo O objetivo deste estudo foi avaliar os efeitos do esterco de ovelha em solos agrícolas no comportamento de Folsomia candida e no crescimento e desenvolvimento inicial de Avena sativa. Para isso, um Latossolo foi submetido a diferentes doses de esterco de ovelha e posteriormente avaliado quanto ao comportamento de fuga e a sobrevivência de Folsomia candida por meio de ensaios ecotoxicológicos padronizados, desempenho inicial da aveia pelo teste de germinação e taxa respiratória basal do solo pela metodologia da respirometria. Houve um aumento na taxa de respiração basal do solo pela aplicação de esterco de ovelha e isso foi consistente com o aumento das doses. A taxa de sobrevivência e o comportamento de fuga dos colêmbolos não foram alterados e não houve alteração no desempenho inicial da aveia, indicando que esse esterco pode ser usado para fertilização orgânica de solos com baixo potencial poluente no solo.

Abstract The aim of this study was to evaluate the effects of sheep manure in agricultural soils on the behavior of Folsomia candida and initial growth and development of Avena sativa. For this, an Oxisol was submitted to different doses of sheep manure and was subsequently evaluated for Folsomia candida survival and avoidance behavior through standardized ecotoxicological assays, the initial performance of oats by germination test and the soil basal respiration rate by respirometry methodology. There was an increase in the basal respiration rate of the soil by the application of sheep manure and this was consistent with the increase of the doses. The survival rate and avoidance behavior of springtails were not altered and there was no change in the initial performance of oats, indicating that this manure can be used for organic fertilization of soils with low soil pollutant potential.

RESUMEN Se estudió la diversidad, riqueza y abundancia de Collembola, en el dosel de un bosque mesófilo en el Estado de Hidalgo, México, durante las estaciones lluviosa y seca de 2004 y 2005, mediante fumigaciones con Piretrinas naturales en tres áreas: una en la zona de borde cerca del camino, otra ubicada a una distancia intermedia, y la tercera en la zona más interna y conservada. Se recolectó un total de 5267 colémbolos representando once familias y 22 géneros, de ellos 4377 (83 %) corresponden a la temporada de lluvias, y 890 (17 %) a la de sequía. Las familias con mayor abundancia fueron Hypogasturidae (con casos como Schoettella distincta y Xenylla sp.), Entomobryidae (Entomobrya sp., Orchesella sp. y Willowsia mexicana), Paronellidae (Salina sp.) y Dicyrtomidae (Dicyrtoma sp.). En la zona interna se recolectaron 1586 (30 %) colémbolos y la mayor abundancia se registró en la zona intermedia, con 2157 (41 %) especímenes. Los valores menores fueron para el área de borde con 1527 (29 %) ejemplares. Se calcularon también los índices de diversidad y equitatividad, y el efecto de la temporada de recolección y sitio sobre la abundancia. Se encontraron diferencias significativas en la abundancia, composición y diversidad de las comunidades en las dos estaciones, y el efecto de borde también tiene una influencia significativa en la composición de la comunidad de colémbolos del dosel, con mayor cantidad de grupos considerados como cosmopolitas en la zona de borde.

ABSTRACT Diversity, richness, and abundance of Collembola communities from the canopy of a montane cloud forest at Hidalgo State, Mexico were studied, through the rainy and drought seasons in 2004 and 2005, using natural piretrine insecticide in three areas: one at the border close to the road, a second one at an intermediate distance, and a third one in the internal and better preserved area. A total of 5267 springtails from eleven families and 22 genera were obtained, of them, 4377 (83 %) belong to the rainy season, and 890 (17 %) at drought. Most abundant families were Hypogasturidae (with cases such as Schoettella distincta and Xenylla sp.), Entomobryidae (Entomobrya sp., Orchesella sp. and Willowsia mexicana), Paronellidae (Salina sp.), and Dicyrtomidae (Dicyrtoma sp.). In the internal area, 1586 (30 %) springtails were collected, the highest abundance was presented at the intermediate area with 2157 (41 %) specimens. The lowest amount was recorded at the border area with 1527 (29 %). Diversity and equitability indexes were calculated and the effect of sampling season and site on the abundance of Collembola were estimated. A significant variation in composition, abundance, and diversity of the community was observed between both seasons, and the border effect has a significant influence in springtails composition in the canopy, with more cosmopolite groups in the border area.

Resumen Introducción: Los colémbolos epiedáficos participan en una amplia variedad de servicios ecosistémicos relacionados con la disponibilidad de nutrientes para las plantas, el almacenamiento y la regulación del agua, la estabilidad del suelo y el control de la humedad y el pH necesarios para la fertilidad de los suelos. Por esta razón, los colémbolos epiedáficos se consideran como organismos sensibles a los cambios generados en el uso del suelo. Objetivo: Comparar la fauna epiedáfica de dos tipos de vegetación introducida: pastos y plantaciones de Eucalyptus pellita en una región de los Llanos Orientales de Colombia. Métodos: La recolección de los colémbolos se realizó en áreas con cobertura de pastos introducidos y áreas forestadas de diferentes edades, con trampas pitfall que contenían Propilenglicol al 40 % como preservante; durante la época seca y la época húmeda. Se comparó la riqueza efectiva de géneros y se explicó su composición en función de 14 variables ambientales y dasométricas mediante un análisis de redundancia (RDA). Resultados: La colembofauna estuvo compuesta por Entomobryomorpha (62 %), Poduromorpha (17 %) y Symphypleona (21.1 %) representados en 12 familias y 26 géneros. La riqueza efectiva de géneros fue significativamente mayor en los pastos que en las plantaciones de E. pellita, pero la abundancia no disminuye significativamente, con excepción de las plantaciones jóvenes durante la época seca. El ensamblaje de colémbolos observados en el área se relacionó principalmente con el desarrollo de cobertura forestal, la disponibilidad de materia orgánica alrededor de la trampa y el contenido de Nitrógeno en el suelo. Conclusiones: Las áreas forestadas permitieron un ensamblaje de colémbolos, menos diverso que las pasturas, pero compuesto tanto por especies adaptadas a áreas abiertas, como de las áreas naturales boscosas circundantes.

Abstract Introduction: Epiedaphic springtails participate in a wide variety of services linked to plant nutrient availability, water storage and regulation, soil stability and control of moisture and pH necessary for soil fertility. Therefore, epiedaphic springtails are sensitive to soil use change. Objective: To compare diversity of epiedaphic springtails in two types of introduced vegetation: pastures and forestations of Eucalyptus pellita in a region of the Colombian eastern plans. Methods: Springtails were collected from introduced pastures and young, middle and mature forested areas of E. pellita, using pitfall traps containing Propylene Glycol (40 %) as preservative, during one dry and one wet season. Alfa and Beta diversity were compared at genus level, and community composition was explained using a set of 14 environmental variables through a redundancy analysis (RDA). Results: Epiedaphic springtails were represented by Entomobryomorpha (62 %), Poduromorpha (17 %) and Symphypleona (21.1 %), 12 families and 26 genera. Effective number of Collembola genera was lower in plantations but abundance was different only in young plantations during dry season. The variables related to forest coverage, soil organic matter coverage and Nitrogen better explained the observed genera assemblage. Conclusions: Forest plantations allowed an assembly of springtails less diverse than in pastures; but comprised both types of genera, those proper of open areas and those from surrounding natural wooded areas.

RESUMEN El glifosato es el herbicida más utilizado a nivel mundial. Sin embargo, los efectos de esta molécula sobre poblaciones no objetivo aún es tema de estudio. La presente investigación tuvo como objetivo determinar el efecto de la aplicación de diferentes dosis de glifosato sobre la variación de las poblaciones de colémbolos. Para esto, se recolectaron muestras de un sustrato orgánico que contenía diferentes poblaciones de colémbolos. Las muestras se llevaron al laboratorio y se aclimataron durante 48 h. Después se les aplicó glifosato C14 en dosis equivalentes a 0 L ha-1, 2 L ha-1, y 4 L ha-1 bajo un diseño experimental completamente al azar, con tres tratamientos y cinco repeticiones. Se realizaron conteos poblacionales implementando el método de flotación a los 0, 4, 7 y 11 d después de aplicación (DDA). Se encontró una distribución de individuos en las familias Isoto-midae y Entomobryidae, divididos en especies de los géneros Proisotoma (Bõrner), Lepidocyrtus (Bourlet) y Seira (Lubbock). Se reportó una disminución entre el 40% y 60% en el número de artrópodos para los tratamientos donde se utilizó el herbicida a los 4 y 7 DDA, mostrando una disminución del tamaño de la comunidad en aquellos tratamientos en los cuales se aplicó el herbicida en relación con el testigo. Sin embargo, no se observaron diferencias entre las dosis de herbicida. Adicionalmente se demostró la presencia de glifosato C14 en los individuos muertos; esto confirma un posible efecto del herbicida sobre algunos sistemas biológicos que llevo a una disminución del tamaño de la población.

ABSTRACT Glyphosate is the most widely used herbicide worldwide. However, the effects of this molecule on non-target populations are still a subject of study. The objective of this research was to determine the effect of the application of different glyphosate doses on variation in collembolan (springtail) populations. To accomplish this goal, samples of organic substrate that contained different collembolan populations were collected. Samples were taken to the laboratory and acclimatized for 48 h. Glyphosate C14 was then applied to the samples in doses equivalent to 0 L ha-1, 2 L ha-1, and 4 L ha-1 under a completely randomized experimental design with three treatments and five replicates. Population counts were performed by implementing the flotation method at 0, 4, 7 and 11 d after application (DAA). We found that individuals were distributed in the families Isotomidae and Entomobryidae and divided into species of the genus Proisotoma (Borner), Lepidocyrtus (Bourlet) and Seira (Lubbock). A decrease in the number of arthropods between 40% and 60% was reported for the treatments with herbicide application at 4 and 7 DAA, showing a drop in the size of the community in those treatments in which the herbicide was applied compared to the control. However, no differences were observed between herbicide doses. Additionally, the presence of glyphosate C14 was demonstrated in dead individuals. This confirms a possible effect of the herbicide on some biological systems that led to a decrease in the size of the population.

RESUMO: O processo de identificação de uma área contaminada envolve uma avaliação preliminar que consiste em análise química e comparação com valores orientadores. No Brasil, esses valores constam na Resolução CONAMA no 420/2009, baseados em análises químicas e fitotoxicidade. O objetivo deste trabalho foi avaliar a ecotoxicidade do cobre em dois solos naturais do Estado do Rio de Janeiro, um Planossolo Háplico (arenoso) e um Argissolo Vermelho-Amarelo (textura média), para verificar se os valores estabelecidos pela legislação oferecem proteção à fauna do solo. Foram realizados ensaios de letalidade e de reprodução com minhocas da espécie Eisenia andrei e com colêmbolos da espécie Folsomia candida. Os resultados indicaram no Planossolo valores de cobre para E. andrei de CL50 435 mg kg-1 e CE50 29 mg kg-1 e para F. candida CL10 137 mg kg-1 e EC50 117 mg kg-1. No Argissolo esse resultado foi para E. andrei CL50 690 mg kg-1 e CE50 61 mg kg-1, e para F. candida CL10 42 mg kg-1 e CE50 138 mg kg-1. Os valores encontrados são menores que o valor de investigação (200 mg kg-1) estabelecidos pela resolução 420/09 do CONAMA, indicando que concentrações menores do que os valores-limite podem afetar esses organismos, a depender do tipo de solo. As minhocas mostraram-se mais sensíveis que os colêmbolos à contaminação por cobre, e o solo arenoso mostrou-se mais suscetível a apresentar ecotoxicidade diante de uma contaminação por cobre, devida, provavelmente, à maior biodisponibilidade do metal. Os ensaios de ecotoxicidade devem ser considerados no desenvolvimento de valores orientadores para solos.

ABSTRACT: The process of identifying a contaminated area involves a preliminary assessment consisting of chemical analysis and comparison with guideline values. In Brazil, these values are included in the CONAMA Resolution no. 420/2009, based on chemical analysis and phytotoxicity. The objective of this research was to evaluate the ecotoxicity of copper in two natural soils in the state of Rio de Janeiro, a Haplic Planosol (sandy) and a Red-Yellow Argisol (medium texture), to verify if the values established by the legislation offer protection to the soil fauna. Lethality and reproduction tests were performed with Eisenia andrei worms and Folsomia candida springtails. Results indicated copper values in Planosol as LC50 435 mg kg 1 and EC50 29 mg kg 1 for E. Andrei and LC10 137 mg kg 1 and EC50 117 mg kg 1 for F. candida. In Argisol, these results were LC50 690 mg kg 1 and EC50 61 mg kg 1 for E. andrei, and LC10 42 mg kg 1 and EC50 138 mg kg 1 for F. candida. Values reported are lower than the research value (200 mg kg-1) established by the CONAMA resolution 420/09, indicating that concentrations lower than the limit values may affect these organisms, depending on the type of soil. Worms were more sensitive than springtails to copper contamination, and sandy soil was more susceptible to ecotoxicity due to copper contamination, probably due to the greater bioavailability of the metal. Results of such ecotoxicity tests should be considered in the development of soil guideline values.

Abstract The afforestation of savannas, previously dedicated to extensive cattle ranching, with fast-growing forest species is a growing phenomenon in the Colombian Orinoquía. In order to estimate changes in the springtail communities due to the establishment of Acacia mangium in the savannas, their diversity in plantations of 1, 3 and 5 years was compared with savannas in a forestry project located in the municipality of Puerto Gaitán (Meta), during the dry and rainy season. Samples were obtained using pitfall traps exposed for 9 days in each season. Springtails richness and diversity among different plantations ages and savannas was estimated and compared in each season. Additionally, the composition was explained based on physicochemical variables of the soil and variables related to the structure of the plantations and management (RDA). Savanna afforestation produced changes in diversity and springtail assemblages. Differences among plantations and savannas were explained by changes in soil temperature and moisture, amount of clay, organic carbon, soil compaction and height of the plantation. It is concluded that early stages of the afforestation of savannas with A. mangium favors the diversity of springtails.

Resumen La forestación de sabanas, previamente dedicadas a la ganadería extensiva, con especies de rápido crecimiento es un fenómeno creciente en la Orinoquía Colombiana. Con el fin de estimar cambios en las comunidades de colémbolos por el establecimiento de Acacia mangium en las sabanas, se comparó la diversidad en plantaciones de 1, 3 y 5 años con la de sabanas del proyecto forestal en el municipio de Puerto Gaitán (Meta), en las temporadas seca y de lluvias. Las muestras fueron obtenidas mediante trampas de caída expuestas durante 9 días en cada época. Se estimó y comparó la riqueza y diversidad de colémbolos entre edades y sabanas en cada temporada. Adicionalmente, se explicó la composición en función de variables fisicoquímicas del suelo y variables relacionadas con la estructura de las plantaciones, mediante el uso de ordenación (RDA). La forestación de sabanas produjo cambios en la diversidad y los ensamblajes de colémbolos. Las diferencias entre plantaciones y sabanas fueron explicadas por cambios en temperatura y contenido de humedad del suelo, contenido de arcilla, carbono orgánico, compactación y altura de la plantación. Se concluye que estados tempranos de la forestación de sabanas con A. mangium favorecen la diversidad de colémbolos.

Abstract The production model used in Brazilian pig farms promotes a concentration of animals in small territorial extensions, causing difficulty in disposing of the manure generated, since the economically viable alternative is to use this as an agricultural fertilizer. The objective was to evaluate the effect of swine manure doses on the biological and behavioral parameters in Folsomia candida by ecotoxicological tests. An Ultisol, Oxisol and Tropical Artificial Soil (TAS) were contaminated with 0, 25, 50, 75 and 100 m3 ha-1 of swine manure, to which springtails were exposed for evaluation of mortality, and the doses 0, 10, 15, 20 and 25 m3 ha-1 of swine manure to evaluated reproduction and avoidance. Lethality was observed in all doses as well as all soils, indicating toxicity of the manure. In the reproduction tests dose since 10 m3 ha-1 caused a reduction of juveniles in all soils. Avoidance behavior was observed in all doses of the Oxisol treatments and at 20 and 25 m3 ha-1 in Ultisol treatments. However, in TAS occurred not avoidance response of F. candida. We conclude that the swine manure was toxic to F. candida and the toxicity is dependent on the soil characteristics and the manure concentrations applied.

Resumo: O objetivo deste trabalho foi avaliar a qualidade do solo de florestas nativas, reflorestamento de eucalipto, pastagem, sistemas de integração lavoura-pecuária e plantio direto, correlacionando a diversidade morfológica de colêmbolos com propriedades físicas e químicas do solo. Amostras de colêmbolos foram coletadas de solos do planalto sul do Estado de Santa Catarina no Brasil, durante o inverno e o verão, usando armadilhas de queda, em grade amostral de 3 × 3. A morfotipagem dos colêmbolos consistiu na observação de cinco características e para cada uma foi atribuído um valor parcial do índice eco-morfológico para a obtenção do Índice de Qualidade do Solo modificado. As avaliações foram da abundância e diversidade de morfotipos e de atributos físicos (umidade do solo, densidade do solo, bioporos, microporosidade e macroporosidade) e químicos do solo (pH em água, relação cálcio/magnésio e carbono orgânico total), descrevendo os resultados por análises de variância e multivariada. A abundância e a diversidade de colêmbolos foram influenciadas pelos diferentes sistemas de uso do solo. Maior índice de qualidade do solo foi determinado em floresta nativa seguida de reflorestamento de eucalipto, pastagem, plantio direto e integração lavoura-pecuária, no inverno. Além disso, encontrou-se maior índice de qualidade do solo em floresta nativa seguida de integração lavoura-pecuária, reflorestamento de eucalipto, plantio direto e pastagem, no verão. Portanto, o índice de qualidade do solo pode ser avaliado pelas características morfológicas de colêmbolos em correlação com as propriedades físicas e químicas, como razão cálcio/magnésio, teores de carbono orgânico total, bioporos, macroporosidade, microporosidade, umidade do solo, densidade do solo e pH.

Abstract: The aim of this work was to evaluate the soil quality of native forest, eucalyptus plantations, pasture, integrated crop-livestock, and no-tillage systems, correlating the morphological diversity of springtails with physical and chemical soil properties. Springtail samples were captured from soils of the southern plateau of the State of Santa Catarina in Brazil, during winter and summer, by using Pitfall traps, using a 3 × 3 point grid. The morphotyping of springtails consisted of the observation of five traits and for each one a partial value of the eco-morphological index was assigned to obtain the modified Soil Quality Index. A correlation of the morphotype abundance and diversity with physical (soil moisture, bulk density, biopores, microporosity, and macroporosity) and chemical (pH in water, calcium/magnesium ratio and total organic carbon content) soil properties was studied, describing all results by variance and multivariate analyses. The springtail abundance and diversity were influenced by the different land use systems. Higher soil quality index was determined in native forest followed by eucalyptus plantations, pasture, no-tillage system and integrated crop-livestock, in the winter. Moreover, higher soil quality index was found in native forest followed by integrated crop-livestock, eucalyptus plantations, no-tillage system and pasture, in the summer. Therefore, the quality index of a soil can be evaluated by the springtail morphological traits in correlation with the physical and chemical properties such as calcium/magnesium ratio, total organic carbon contents, biopores, macroporosity, microporosity, soil moisture, bulky density and pH.

Resumo: O objetivo deste estudo foi avaliar a influência do uso do solo na diversidade morfológica de colêmbolos e verificar a relação destes organismos com os atributos físicos, químicos e microbiológicos do solo. Foram coletadas amostras na região Sul de Santa Catarina, envolvendo três municípios: Orleans, Lauro Müller e Siderópolis, nos sistemas de uso do solo (SUS) de floresta nativa (FN), reflorestamento de eucalipto (RE), pastagem perene (PA), integração lavoura-pecuária (ILP) e plantio direto (PD). As coletas de solo para determinação dos atributos edáficos e a instalação de armadilhas de queda, foram realizadas no inverno e verão, nos mesmos pontos. Os colêmbolos coletados foram contados e morfotipados e os dados foram analisados por meio da abundância, diversidade de Shannon-Wiener (H') e Margalef, índice de dominância de Simpson (D), uniformidade de Pielou (J), riqueza de morfotipos e Análise de Componentes Principais (ACP). Os morfotipos de Collembola foram influenciados pelas condições de manejo de cada sistema, sobretudo o impacto dos SUS nesses organismos depende da intensidade das práticas florestais/agrícolas utilizadas, incluindo rotações culturais e preparo do solo.

Abstract: The aim of this study was to evaluate the influence of soil use on the morphological diversity of springtails and to verify the relationship of these organisms with soil physical, chemical, and microbiological properties. Samples were collected in the Southern region of Santa Catarina, involving three municipalities: Orleans, Lauro Müller, and Siderópolis, in the land use systems (LUS) of native forest (NF), Eucalyptus plantation (EP), pasture (PA), integrated crop-livestock (ICL), and no-tillage (NT). Soil samples to determine edaphic properties and pitfall traps were collected in winter and summer, in the same areas. The collected springtails were counted and morphotyped and the data were analyzed through abundance, Shannon-Wiener diversity (H') and Margalef index, Simpson dominance index (D), Pielou evenness index (J), morphotype richness, and Principal Components Analysis (PCA). Springtails morphotypes were influenced by the management conditions of each system, especially the impact of LUS on these organisms depends on the intensity of the forest/agricultural practices used, including crop rotation and soil preparation.

ABSTRACT: In recent years, various studies and development using nanoparticles (NPs) have been carried out in the most diverse areas of knowledge. Although nanomaterials are widely employed by many sectors and some may have a fertilizing potential, little is known about their effects on the environment. This study aimed to evaluate the effect of applying, in tropical natural soil, different contents of nanoparticles of zinc oxide (NPs-ZnO) and non-nano zinc oxide (ZnO) on soil pH and on the survival and reproduction rates of earthworms ( Eisenia andrei ) and springtails ( Folsomia candida ) through standardized ecotoxicological tests. The tests used a tropical soil representative of Brazil, classified as Entisol ( Neossolo Quartzarênico órtico típico ) with no history of agricultural use, collected in the 0.00-0.20 m layer, previously sieved (2-mm mesh) and defaunated. The experimental design was completely randomized, and treatments consisted of two forms of zinc (Zn), NPs-ZnO and ZnO, at the following doses: 0, 50, 100, 200, 400, 800, 2,000, and 4,000 mg kg−1. Standardized ecotoxicological tests showed no toxicity of NPs-ZnO in terms of lethality of E. andrei and F. candida . In E. andrei reproduction tests, NPs-ZnO were toxic at doses higher than 400 mg kg−1 (EC50 of 1,021 mg kg−1). Tests with F. candida demonstrated that its reproduction rate was significantly affected by NPs-ZnO at a rate of 4,000 mg kg−1 (EC50 of 3,636 mg kg−1). When used in Entisol, the NPs-ZnO inhibit the reproduction of earthworms and springtails; earthworms are more sensitive to such an effect, it being demonstrate at lower contents than those found for springtails.

ABSTRACT: We studied the predatory behavior of two spider species of the genus Phonotimpus (Araneae: Phrurolithidae) when confronted with four different types of potential prey (one Collembola, two Hemiptera and one Psocoptera). These spiders live in the soil leaf litter of a cloud forest and coffee orchards in Chiapas, Mexico. There are no previous studies of the hunting behavior for this spiders’ family. Due to the small size of the spiders, the study was made in laboratory conditions with video recordings of the encounters with each prey type. From the analyses of the video recordings we established a catalog of 16 behavioral acts that the spiders displayed in the encounters with prey, among these we characterized “waving” and “attack”. The recordings showed that both spider species had significant higher aptitudes to attack and capture springtails than hemipterans and psocopterans, the spiders displayed a richer behavioral repertoire when confronted the springtails and, for Phonotimpus pennimani a higher activity level when confronting the springtails. Thus, our results are similar to other studies on prey consumption in laboratory conditions by two different species of Phrurolithidae, pointing as highly possible that, in its natural habitat, springtails are an important part of the prey spectrum of the Phrurolithidae. The recordings also showed that both spider species employ two hunting strategies. One is ambushing, where the spider remains fixed in one place for relatively long periods, waiting for the approach of a potential prey, and the other is active searching, where the spider actively wanders its milieu until it encounters a potential prey. In terms of the distribution of times during encounters, ambushing predominated over active searching.

RESUMEN: Se estudió el comportamiento depredador de dos especies de arañas del género Phonotimpus (Araneae: Phrurolithidae) frente a cuatro tipos diferentes de presas potenciales (un Collembola, dos Hemiptera y un Psocoptera). Estas arañas habitan en la hojarasca del suelo de un bosque de niebla y de un cafetal en Chiapas, México. No existen estudios previos sobre el comportamiento de caza de esta familia de arañas. Debido al reducido tamaño de las arañas, el estudio se realizó en laboratorio, mediante videograbaciones de los encuentros con cada tipo de presa. A partir de las grabaciones se elaboró un catálogo de 16 actos de comportamiento que las arañas presentaron durante los encuentros con las presas, entre los que destacan el de “abanicar” y el de “ataque”. Las grabaciones evidenciaron que ambas especies de arañas tuvieron una capacidad significativamente mayor de ataque y captura de los colémbolos que de los hemípteros y psocópteros, además de presentar un repertorio más variado de actos frente a los colémbolos y, para Phonotimpus pennimani un mayor nivel de actividad durante los encuentros con colémbolos. Así, nuestros resultados son similares a los de otros autores que estudiaron el consumo de presas en laboratorio, por dos especies distintas de Phrurolithidae, indicando que es altamente posible que en su hábitat natural los colémbolos sean parte importante de las presas de las Phrurolithidae. Las grabaciones también mostraron que ambas especies de arañas emplean dos estrategias de caza. Una es la emboscada, donde la araña permanece fija en un sitio por periodos relativamente prolongados, a la espera de la aproximación de una presa potencial, y la otra es la búsqueda activa, en la que la araña recorre activamente su entorno hasta que se topa con una presa potencial. De las dos estrategias, hubo un claro predominio de la emboscada, en cuanto su distribución de tiempos durante los encuentros.

Resumo Nesse trabalho são apresentadas informações sobre a ocorrência e a caracterização morfológica de galhas induzidas por insetos em uma área de Cerrado do Oeste da Bahia, Brasil. Os materiais foram coletados mensalmente durante duas horas entre Março e Setembro de 2015. Foram encontrados quinze morfotipos de galhas em doze espécies vegetais, distribuídas em sete famílias. A família de planta com maior riqueza de galhas foi Fabaceae (n = 8). Com relação à morfologia externa, foram encontradas galhas com forma globoide, lenticular, enrolamento marginal, cônica, cilíndrica, fusiforme, esférica e bolso. Quanto à fauna associada às galhas, os morfotipos globoide, cônico, lenticular, fusiforme, esférico e cilíndrico foram induzidos por Cecidomyiidae. Além disso, espécies de microhimenópteros pertencentes às famílias Eulophidae, Eurytomidae e Encyrtidae foram encontrados. A indução de galhas por Thysanoptera, nos morfotipos enrolamento marginal e formato de bolso, também foi detectada. Espécies de colembôlos foram identificadas como sucessoras. Espécies não descritas de Schizomyia e Lopesia foram registradas em Bauhinia cupulata e Andira humilis, respectivamente.

Abstract In this study, we report the first records and morphological characterization of galls in a Cerrado area in western Bahia, Brazil. The data were collected monthly over two hours between March and September 2015. Fifteen gall morphotypes were found in twelve plant species distributed among seven families. The plant family with the greatest richness of galls was Fabaceae (n = 8). The following gall morphologies were found: globoid, lenticular, marginal leaf roll, conical, cylindrical, fusiform, spherical and pocket shaped. Cecidomyiidae induced globoid, lenticular, conical, fusiform, spherical and cylindrical morphotypes. In addition, species of microhymenoptera belonging to the Eulophidae, Eurytomidae and Encyrtidae families were found. Marginal leaf roll and pocket-shaped galls induced by Thysanoptera were also verified. Springtails were also identified as a successor. Undescribed species of Schizomyia and Lopesia were recorded in B. cupulata and Andira humilis, respectively.

RESUMEN Objetivo. El objetivo de este estudio fue evaluar la influencia de la suplementación subcutánea con diselenuro de difenilo (PhSe)2 en ovejas lecheras infectadas con nematodos sobre la salud animal y posible daño al ambiente cuando las heces de estos animales se utilicen para fertilizar. Materiales y métodos. El experimento se realizó utilizando 16 ovejas lecheras, que se dividieron en dos grupos: el grupo A se usó como control y el grupo B se suplementó con 3 µmol/kg of (PhSe)2 vía subcutánea. Se utilizaron muestras de sangre para determinar la función hepática, así como el metabolismo de proteínas y lípidos en animales. Las heces se utilizaron para determinar el número de huevos por gramo de heces (EPG), así como para las pruebas de ecotoxicología. Resultados . La suplementación (PhSe)2 no afectó la reproducción de helmintos. Los niveles totales de proteína y globulina aumentan (p<0.05) en los animales suplementados, mientras que los niveles séricos de alanina aminotransferasa (ALT) disminuyen (p<0.05) al final del diseño experimental. Los niveles de colesterol aumentan (p<0.05) en los animales suplementados, mientras que los triglicéridos, la albúmina y la urea no difieren entre los grupos (p>0.05). Las heces de ovejas suplementadas no interferían en la reproducción de las colas de caballo. Conclusiones. A la dosis administrada, el (PhSe)2 no es capaz de controlar el parasitismo; sin embargo, aumenta los niveles de globulinas y colesterol, que son importantes para la respuesta inmune y para la reproducción, respectivamente. Las heces de animales suplementados pueden usarse como fertilizantes orgánicos, sin impactos negativos en ambiente.

ABSTRACT Objective. The aim of this study was to evaluate the influence of subcutaneous supplementation with diphenyl diselenide ((PhSe)2) in dairy sheep infected with gastrointestinal nematodes on animal health and possible damage to environment when the feces of these animals will be used for fertilizing. Materials and methods. The experiment was performed using 16 primipara dairy sheep, that were divided into two groups: the group A as control and the group B supplemented with 3 µmol/kg of (PhSe)2 subcutaneously. Blood samples were used to determine the hepatic function, as well as the protein and lipid metabolism in animals. Feces were used to determine the number of helminths eggs per gram of feces (EPG), as well as used for ecotoxicology tests. Results. The (PhSe)2 supplementation not affected the helminths reproduction, since the EPG did not differ (p>0.05) between groups. Total protein and globulin levels increase (p<0.05) in supplemented animals, while the seric alanine aminotransferase (ALT) levels decrease (p<0.05) in the end of experimental design. Cholesterol levels increase (p<0.05) in the supplemented animals, while triglycerides, albumin and urea not differ between groups (p>0.05). The feces of supplemented sheep not interfered the springtails reproduction. Conclusions. At the administered dose, the (PhSe)2 is not able to control the parasitism, however, it did increase the globulins and cholesterol levels, that are important to immune response and for sheep reproduction, respectively. Also, the feces of supplemented animals with (PhSe)2 can be used as organic fertilizing, without negative impacts to environment.