The present work focused on the screening process of different inorganic oxides and different temperatures for the epoxidation reaction of macaw palm vegetable oil without using solvent, a promising Brazilian biomass that can substitute other vegetable oils (e.g., soybean oil) in technological applications. This oil has an iodine value of 108.48 g of I2 per 100 g being categorized as a semi-dry oil. The epoxidized vegetable oil can be applied in manifold areas in polymer science. The epoxidation reaction here presented avoided organic solvents and organic acids and aimed to produce a greener route using metal oxides or niobium phosphate as a peroxide-activating catalyst. The experimental parameters were maintained as 10 mol% of catalyst loading and a time of 24 h; however, the temperature for some catalysts was changed to improve the epoxide conversion. Experiments were measured by proton nuclear magnetic resonance (1H NMR). Experiments using titanium oxide (TiO2) as a catalyst reached a conversion greater than 84.7%, while experiments using aluminium oxide (Al2O3) or lanthanum oxide (La2O3) achieved conversions of 22.6 and 30.1%, respectively. Furthermore, it was observed that both niobium compounds instantly react with hydrogen peroxide to form peroxy derivatives. solvent e.g., eg e (e.g. applications 10848 108 48 108.4 I semidry semi dry science peroxideactivating activating 1 mol 2 h however 1H H NMR. NMR . NMR) TiO2 TiO (TiO2 847 84 7 84.7% Al2O3 AlO Al O (Al2O3 La2O3 LaO La (La2O3 226 22 6 22. 301 30 30.1% respectively Furthermore derivatives e.g. (e.g 1084 4 108. (TiO 8 84.7 Al2O (Al2O La2O (La2O 3 30.1 e.g 84. 30.

The Schröeder-van Laar equation to predict the theoretical eutectic melting temperature values was applied to a set of fourteen binary mixtures (UR1 to UR14). The fourteen binary mixtures belong to a structurally diverse group of organic molecules. They are represented by the 5-membered heterocycles containing N-S and N-O rings, flexible methylene chains (RH), semi-rigid semi-fluorinated chains (RF), and hydrogen bonds (HB). A virtual screening totalizing ninety-one possible theoretical combinations was planned. The UR1 to UR14 were prepared by mixing individual components using the calculated mole fractions. The experimental (Te) and theoretical (Tt) temperatures for the eutectic melting points were determined using polarized optical microscopy (POM) and differential scanning calorimetry (DSC) techniques. Heterogeneous mixtures were formed for components with large structural diversity as seen by POM. During the melting of the mixtures, irregular behavior through the segregation of the components was recorded. The study showed that the similarities in structural diversities favor the formation of homogeneous mixtures, with Te values approaching Tt, as predicted by the Schröeder-van Laar equation and by the theory of eutectic mixtures. Liquid crystal properties were affected in homogeneous binary mixtures containing one of the individual liquid crystal components. Schröedervan Schröeder van UR (UR UR14. . UR14) molecules 5membered membered 5 NS N S NO O rings RH, RH , (RH) semirigid semi rigid semifluorinated fluorinated RF, RF (RF) HB. HB (HB) ninetyone ninety planned fractions (Te Tt (Tt POM (POM DSC (DSC techniques recorded (RH (RF (HB

Worldwide, millions of people suffer from neurological and psychiatric conditions, such as schizophrenia, depression, and neurodegenerative diseases. These conditions are often linked to hyperactivity of monoamine oxidase B (MAO-B) in the brain, and inhibiting this enzyme can reduce oxidative stress, stabilize neurotransmitter levels, and alleviate symptoms. In this context, a pharmacophoric model was created using PharmaGist with 31 hits extracted from BindingDB to enable a pharmacophoric-based search of potential MAO-B inhibitors in ZincPharmer’s chemical space, followed by the building of 10 conformers for each molecule in the software OMEGA, screening for structural and electrostatic similarity to safinamide using ROCS (Rapid overlay of chemical structures) and EON (Electrostatic similarity for lead-hopping), respectively. In addition, the molecular docking with Gold using the score function was performed to rank the ligands with the highest probabilities of affinity to MAO-B and then discriminate those with suitable physical chemical, pharmacokinetic, and toxicological features using the tools QikProp, SwissADME, and DEREK. Four potential ligands with selective MAO-B inhibitor properties were identified, which show promise as future therapeutic agents for MAO-B-related disorders. Worldwide schizophrenia depression diseases MAOB MAO (MAO-B brain stress levels symptoms context 3 pharmacophoricbased based ZincPharmers ZincPharmer s space 1 OMEGA Rapid structures Electrostatic leadhopping, leadhopping lead hopping , lead-hopping) respectively addition pharmacokinetic QikProp SwissADME DEREK identified MAOBrelated related disorders lead-hopping

RESUMO: O Rio Grande do Sul possui uma grande diversidade de espécies de répteis distribuídos por todo o Estado. Devido às ações antrópicas, como a fragmentação de habitats, estes animais vêm sendo observados com frequência no meio urbano e periurbano. Isso facilita a disseminação de patógenos entre animais e seres humanos, sendo um risco a saúde única, visto que muitas doenças são consideradas zoonoses. A leptospirose está entre as zoonoses mais comuns do mundo e é causada pelas espécies patogênicas da bactéria do gênero Leptospira. Ainda não se sabe o papel dos répteis no ciclo da doença, porém, estudos sorológicos demonstraram positividade para anticorpos contra Leptospira spp. em cágados e serpentes, o que pode indicar que estes animais atuam na manutenção do patógeno no ambiente. O objetivo deste estudo foi observar a presença de anticorpos anti-Leptospira em répteis oriundos de resgate e levados ao Núcleo de Reabilitação da Fauna Silvestre e Centro de Triagem de Animais Silvestres da Universidade Federal de Pelotas (NURFS-CETAS/UFPEL). As amostras foram coletadas de 55 animais (39 Trachemys dorbigni; 3 Philodryas patagoniensis; 3 Caiman latirostris; 3 Salvator merianae; 2 Acanthochelys spixii; 2 Phrynops hilarii; 2 Hydromedusa tectifera; 1 Philodryas aestiva), durante o período de agosto de 2022 a dezembro de 2023, e testadas para 12 sorovares de referência. Dois animais demonstraram positividade, um deles para o sorovar patogênico Pyrogenes e outro para o sorovar patogênico Canicola. Este resultado demonstra a importância de outras espécies, além dos mamíferos, como potenciais reservatórios e responsáveis pela manutenção da leptospirose no ambiente. RESUMO Estado antrópicas habitats periurbano humanos única doença porém spp serpentes ambiente antiLeptospira anti NURFSCETAS/UFPEL. NURFSCETASUFPEL NURFS CETAS/UFPEL . CETAS UFPEL (NURFS-CETAS/UFPEL) 5 39 (3 dorbigni patagoniensis latirostris merianae spixii hilarii tectifera aestiva, aestiva , aestiva) 202 2023 referência Canicola mamíferos NURFSCETAS NURFSCETAS/UFPEL CETASUFPEL (NURFS-CETAS/UFPEL ( 20

ABSTRACT: The state of Rio Grande do Sul has a great diversity of reptile species distributed throughout its territory. Due to human actions, such as habitat fragmentation, these animals have been frequently observed in urban and peri-urban environments. This facilitates the spread of pathogens between animals and humans, posing a unique health risk, as many diseases are considered zoonoses. Leptospirosis is among the most common zoonoses in the world and is caused by pathogenic species of bacteria of the genus Leptospira. The role of reptiles in the cycle of this disease is yet unknown. However, serological studies have demonstrated positivity for antibodies against Leptospira spp. in tortoises and snakes, which may indicate that these animals act in maintaining the pathogen in the environment. This observed the presence of anti-Leptospira antibodies in rescued reptiles taken to the Center for Rehabilitation of Wild Fauna and Screening Center for Wild Animals at the Universidade Federal de Pelotas (NURFS-CETAS/UFPEL). Samples were collected from 55 animals (39 Trachemys dorbigni; 3 Philodryas patagoniensis; 3 Caiman latirostris; 3 Salvator merianae; 2 Acanthochelys spixii; 2 Phrynops hilarii; 2 Hydromedusa tectifera; 1 Philodryas aestiva) from August 2022 to December 2023 and tested for 12 reference serovars. Two animals demonstrated positivity, one for the pathogenic serovar Pyrogenes and the other for the pathogenic serovar Canicola. This result reflected the importance of different species besides mammals as potential reservoirs and responsible for maintaining leptospirosis in the environment. ABSTRACT territory actions fragmentation periurban peri environments humans risk unknown However spp snakes environment antiLeptospira anti NURFSCETAS/UFPEL. NURFSCETASUFPEL NURFS CETAS/UFPEL . CETAS UFPEL (NURFS-CETAS/UFPEL) 5 39 (3 dorbigni patagoniensis latirostris merianae spixii hilarii tectifera aestiva 202 serovars Canicola NURFSCETAS NURFSCETAS/UFPEL CETASUFPEL (NURFS-CETAS/UFPEL ( 20

Fruits of Schinus terebinthifolia Raddi were investigated. A fast and efficient method for the simultaneous determination of 11 polyphenolic substances by high performance liquid chromatography-diode array detector (HPLC-DAD) was applied. Limits of quantification ranged between 0.78 and 3.14 mg L-1. High concentrations of kaempferol were found. An exploratory evaluation was performed using principal component analysis (PCA) for fruit origin differentiation. Total phenolic content (TPC) ranged from 274 to 517 mg GAE 100 g-1; total flavonoid content (TFC) ranged from 101 to 283 mg QE 100 g-1. In vitro analyses showed that antioxidant capacity with 2,2-diphenyl-1-picrylhydrazyl (DPPH) ranged from 1.7 to 7 µM DPPH 100 g-1. The bacteriostatic and bactericidal effect were also evaluated against: Bacillus subtilis, B. cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella choleraesuis, Candida albicans and C. glabrata, using broth microdilution assay. It can be inferred that S. terebinthifolia Raddi fruit has the potential to act as an ally in the search for new alternatives in the action against pathogens, and it can be used as a potential natural antioxidant and antimicrobial agent. Metals (Cu, Fe, Mg, Mn and Zn) were also determined by flame atomic absorption spectrometry (FAAS), with emphasis on high concentrations of Mg, Zn and Fe. investigated 1 chromatographydiode chromatography diode HPLCDAD HPLC DAD (HPLC-DAD applied 078 0 78 0.7 314 3 14 3.1 L1. L1 L 1. L-1 found PCA (PCA differentiation TPC (TPC 27 51 10 g1 g g-1 TFC (TFC 28 g1. 2,2diphenyl1picrylhydrazyl 22diphenyl1picrylhydrazyl diphenylpicrylhydrazyl 2,2 diphenyl picrylhydrazyl 2 (DPPH 17 subtilis B cereus aureus coli aeruginosa choleraesuis C glabrata assay S pathogens agent Cu, Cu (Cu Fe Mg FAAS, FAAS , (FAAS) 07 0. 31 3. L- 5 g- 2diphenyl1picrylhydrazyl 22 2, (FAAS

ABSTRACT Introduction: body mass index and abdominal perimeter are anthropometric measurements that could be concordant in the Peruvian population for screening for overweight or obesity. Objective: to analyze the correlation and agreement between body mass index and abdominal circumference tests for the detection of overweight or obesity in Peruvian adults. Methodology: observational, analytical and cross-sectional study based on data from the national demographic family health survey. The population was 28,960 adults. The variables were: body mass index and abdominal perimeter. Cramer's V test, student's t, Spearman's correlation and Cohen's kappa were used. Results: there was a strong association between body mass index and abdominal perimeter in women (V=0.701), moderate in men (V=0.632). In Cohen's kappa test, both variables agreed substantially in women (kappa=0.677) and moderately in men (kappa=0.580). Using the Odds Ratio, women with a high body mass index had a 97.22 times greater probability of having a high abdominal circumference, while in men with a high body mass index it was 110.76 times greater. The Spearman correlation was very high and positive in both sexes. Conclusions: abdominal circumference and body mass index are significantly associated and correlated in Peruvian adults; they presented substantial agreement in women and moderate agreement in men. It is suggested to prioritize the use of the abdominal perimeter, because it distinguishes overweight or obesity according to sex, optimizing it by determining a cut-off point adjusted to the Peruvian population.

RESUMEN Introducción: el índice de masa corporal y el perímetro abdominal son medidas antropométricas que podrían ser concordantes en la población peruana para el cribado de sobrepeso u obesidad. Objetivo: analizar la correlación y concordancia entre las pruebas de índice de masa corporal y perímetro abdominal para la detección de sobrepeso u obesidad en adultos peruanos. Metodología: estudio observacional, analítico y transversal basado en datos de la encuesta nacional demográfica de salud familiar. La población fue de 28960 adultos. Las variables fueron: índice de masa corporal y perímetro abdominal. Se utilizó la prueba V de Cramer, t de student, correlación de Spearman y kappa de Cohen. Resultados: hubo una asociación fuerte entre índice de masa corporal y perímetro abdominal en mujeres (V=0,701), moderada en hombres (V=0,632). En la prueba kappa de Cohen, ambas variables concordaron sustancialmente en mujeres (kappa=0,677) y moderadamente en hombres (kappa=0,580). Mediante el Odds Ratio, las mujeres con índice de masa corporal elevado tuvieron una probabilidad 97,22 veces mayor de perímetro abdominal elevado, mientras que en hombres con índice de masa corporal elevado fue 110,76 veces mayor. La correlación de Spearman fue muy alta y positiva en ambos sexos Conclusiones: el perímetro abdominal y el índice de masa corporal están asociados y correlacionados significativamente en adultos peruanos, presentaron una concordancia sustancial en mujeres y moderada en hombres. Se sugiere priorizar el uso del perímetro abdominal, debido a que distingue sobrepeso u obesidad según sexo, optimizándolo mediante la determinación de un punto de corte ajustado a la población peruana.

Abstract Introduction: Chronic kidney disease (CKD) and metabolic syndrome (MS) are recognized as public health problems which are related to overweight and cardiometabolic factors. The aim of this study was to develop a model to predict MS in people with CKD. Methods: This was a prospective cross-sectional study of patients from a reference center in São Luís, MA, Brazil. The sample included adult volunteers classified according to the presence of mild or severe CKD. For MS tracking, the k-nearest neighbors (KNN) classifier algorithm was used with the following inputs: gender, smoking, neck circumference, and waist-to-hip ratio. Results were considered significant at p < 0.05. Results: A total of 196 adult patients were evaluated with a mean age of 44.73 years, 71.9% female, 69.4% overweight, and 12.24% with CKD. Of the latter, 45.8% had MS, the majority had up to 3 altered metabolic components, and the group with CKD showed statistical significance in: waist circumference, systolic blood pressure, diastolic blood pressure, and fasting blood glucose. The KNN algorithm proved to be a good predictor for MS screening with 79% accuracy and sensitivity and 80% specificity (area under the ROC curve – AUC = 0.79). Conclusion: The KNN algorithm can be used as a low-cost screening method to evaluate the presence of MS in people with CKD. Introduction (CKD (MS factors Methods crosssectional cross sectional Luís MA Brazil tracking knearest k nearest (KNN inputs gender smoking circumference waisttohip hip ratio 005 0 05 0.05 19 4473 44 73 44.7 years 719 71 9 71.9 female 694 69 4 69.4 1224 12 24 12.24 latter 458 45 8 45.8 components pressure glucose 79 80 area 0.79. 079 0.79 . 0.79) Conclusion lowcost low cost 00 0.0 1 447 7 44. 71. 6 69. 122 2 12.2 45. 07 0.7 0. 12.

Resumo Introdução: A doença renal crônica (DRC) e a síndrome metabólica (SM) são reconhecidas como problemas de saúde pública relacionados ao excesso de peso e a fatores cardiometabólicos. O objetivo deste estudo foi desenvolver um modelo para prever a SM em pessoas com DRC. Métodos: Este foi um estudo transversal prospectivo de pacientes de um centro de referência em São Luís, MA, Brasil. A amostra incluiu voluntários adultos classificados de acordo com a presença de DRC leve ou grave. Para o rastreamento da SM, o algoritmo de classificação k-nearest neighbors (KNN) foi utilizado com os seguintes dados: sexo, tabagismo, circunferência do pescoço e relação cintura-quadril. Os resultados foram considerados significativos com p < 0,05. Resultados: Foram avaliados 196 pacientes adultos com média de idade de 44,73 anos, 71,9% do sexo feminino, 69,4% com sobrepeso e 12,24% com DRC. Desses últimos, 45,8% apresentaram SM, a maioria tinha até 3 componentes metabólicos alterados, e o grupo com DRC apresentou significância estatística em: circunferência da cintura, pressão arterial sistólica, pressão arterial diastólica e glicemia de jejum. O algoritmo KNN comprovou ser um bom preditor para a triagem de SM com acurácia e sensibilidade de 79% e especificidade de 80% (área sob a curva ROC – AUC = 0,79). Conclusão: O algoritmo KNN pode ser usado como um método de triagem de baixo custo para avaliar a presença de SM em pessoas com DRC. Introdução (DRC (SM cardiometabólicos Métodos Luís MA Brasil grave knearest k nearest (KNN dados tabagismo cinturaquadril. cinturaquadril cintura quadril. quadril cintura-quadril 005 0 05 0,05 Resultados 19 4473 44 73 44,7 anos 719 71 9 71,9 feminino 694 69 4 69,4 1224 12 24 12,24 últimos 458 45 8 45,8 alterados sistólica jejum 79 80 área 0,79. 079 0,79 . 0,79) Conclusão 00 0,0 1 447 7 44, 71, 6 69, 122 2 12,2 45, 07 0,7 0, 12,

Abstract: This research explores the impact of digital books on student’s performance in mathematics. The theoretical arguments of this research are based on the self-regulated learning theory. To employ the complete research strategy, 65 papers were retrieved in the first round of research, including 30 from ERIC, 20 from Science Direct, and 15 from EBSCO. Following that, only 40 papers produced findings from the major section screening. The article’s systematic literature review and thematic analysis of the published material resulted in a sample size of 23 articles for this study. A qualitative thematic analysis Software, NVivo 12, was used to evaluate qualitative data. The findings indicated that motivation, technological advancement, information technology, learning objectives, sources for digital application, challenges of technology, traditional learning style and visual information have a significant collision with students’ mathematical learning.

Resumo: Esta investigação explora o impacto dos livros digitais no desempenho dos alunos em matemática. Os argumentos teóricos desta investigação baseiam-se na teoria da aprendizagem autorregulada. Para aplicar a estratégia completa de pesquisa, foram recuperados 65 artigos na primeira rodada de pesquisa, incluindo 30 do ERIC, 20 do Science Direct e 15 do EBSCO. Em seguida, apenas 40 artigos produziram resultados a partir da triagem da seção principal. A revisão sistemática da literatura e a análise temática do material publicado resultaram em uma amostra de 23 artigos para este estudo. Foi utilizado um software de análise temática qualitativa, o NVivo 12, para avaliar os dados qualitativos. Os resultados indicaram que a motivação, o avanço tecnológico, a tecnologia da informação, os objetivos de aprendizagem, as fontes de aplicação digital, os desafios da tecnologia, o estilo de aprendizagem tradicional e a informação visual têm um impacto significativa na aprendizagem matemática dos alunos.

Resumen: Esta investigación explora el impacto de los libros electrónicos en el rendimiento de los alumnos en matemáticas. Los argumentos teóricos de esta investigación se basan en la teoría del aprendizaje autorregulado. Para emplear la estrategia de investigación completa, se recuperaron 65 artículos en la primera ronda de investigación, incluidos 30 de ERIC, 20 de Science Direct y 15 de EBSCO. A continuación, solo 40 artículos arrojaron resultados en la criba de la sección principal. La revisión sistemática de la literatura y el análisis temático del material publicado dieron como resultado una muestra de 23 artículos para este estudio. Para evaluar los datos cualitativos se utilizó un programa informático de análisis temático cualitativo, NVivo 12. Los resultados indicaron que la motivación, el avance tecnológico, la tecnología de la información, los objetivos de aprendizaje, las fuentes de aplicación digital, los retos de la tecnología, el estilo de aprendizaje tradicional y la información visual tienen una colisión significativa con el aprendizaje matemático de los estudiantes.

Resumo Introdução: Disfunção erétil (DE) é um problema sexual comum entre homens com doença renal crônica (DRC). A gravidade da disfunção sexual tende a piorar com a lesão renal. Este estudo tem como objetivo avaliar função erétil e qualidade de vida sexual de pacientes homens adultos com DRC em hemodiálise (HD) em um hospital na Amazônia brasileira. Métodos: Realizou-se estudo quantitativo transversal no Setor de HD da Unidade de Nefrologia, incluindo homens com DRC ≥ 18 anos, submetidos a ≥ 3 sessões semanais de HD por ≥ 3 meses e sexualmente ativos por ≥ 6 meses. Utilizamos o Quociente Sexual Masculino (QSM) para medir satisfação sexual e o Índice Internacional de Função Erétil (IIEF5) para estabelecer função erétil. A análise estatística foi realizada com o SPSS 21.0 usando testes apropriados, como Mann-Whitney e Kruskal-Wallis (P < 0,05). Resultados: Avaliamos 98 pacientes (51,68 ± 15,28 anos). Eles eram predominantemente casados/ou vivendo com um(a) parceiro(a) (60,20%), com tempo de HD entre 1 e 5 anos (55,10%) e KTV médio de 1,17. A prevalência de DE foi 66,30% e foi associada a maior faixa etária (p = 0,01), menor renda familiar (p = 0,02), diabetes (p = 0,01), menor hemoglobina corpuscular média (p = 0,04), maior cálcio total (p = 0,04) e menor albumina (p = 0,03). Aproximadamente 75% classificaram sua vida sexual como regular a excelente. Conclusão: Apesar da elevada prevalência de DE, a maioria dos homens com DRC em HD relatou vida sexual regular a excelente. O estudo ressalta a importância de estabelecer triagem eficaz e realizar avaliações de rotina sobre questões sexuais nesses homens. Introdução (DE DRC. . (DRC) (HD brasileira Métodos Realizouse Realizou se Nefrologia QSM (QSM IIEF5 IIEF (IIEF5 210 21 0 21. apropriados MannWhitney Mann Whitney KruskalWallis Kruskal Wallis P 0,05. 005 0,05 05 0,05) Resultados 9 51,68 5168 51 68 (51,6 1528 15 28 15,2 anos. anos) casadosou casados ou uma um(a parceiroa parceiro parceiro(a 60,20%, 6020 60,20% , 60 20 (60,20%) 55,10% 5510 55 10 (55,10% 117 17 1,17 6630 66 30 66,30 p 0,01, 001 0,01 01 0,01) 0,02, 002 0,02 02 0,02) 0,04, 004 0,04 04 0,03. 003 0,03 03 0,03) 75 excelente Conclusão (DRC (IIEF 2 00 0,0 51,6 516 (51, 152 15, 602 60,20 (60,20% 55,10 551 (55,10 11 1,1 663 66,3 7 0, 51, (51 60,2 (60,20 55,1 (55,1 1, 66, (5 60, (60,2 55, (55, ( (60, (55 (60 (6

Abstract Introduction: Erectile dysfunction (ED) is a common sexual problem among men with chronic kidney disease (CKD). The severity of sexual dysfunction tends to worsen with kidney damage. This study aims to evaluate the erectile function and sexual quality of life of adult male CKD patients undergoing hemodialysis (HD) in a hospital located in the Brazilian Amazon. Methods: A cross-sectional quantitative study was performed within the HD Sector of the Nephrology Unit including men with CKD aged ≥ 18 years, undergoing ≥ 3 weekly HD sessions for ≥ 3 months who had been sexually active for ≥ 6 months. We used the Male Sexual Quotient (MSQ) to measure sexual satisfaction and the International Index of Erectile Function (IIEF5) to establish erectile function. Statistical analysis was performed with SPSS 21.0 using appropriate tests, such as Mann-Whitney and Kruskal-Wallis (P < 0.05). Results: Ninety-eight patients (51.68 ± 15.28 years) were evaluated. They were primarily married/or living with a partner (60.20%), with HD time between 1 to 5 years (55.10%), and an average KTV of 1.17. ED prevalence was 66.30%, and it was associated with a higher age group (p = 0.01), lower family income (p = 0.02), diabetes (p = 0.01), lower mean corpuscular hemoglobin (p = 0.04), higher total calcium (p = 0.04), and lower albumin (p = 0.03). Around 75% classified their sex life as regular to excellent. Conclusion: Despite the high ED prevalence, most men with CKD in HD reported experiencing regular to excellent sex life. The study underscores the importance of establishing effective screening and conducting routine evaluations regarding sexual issues in these men. Introduction (ED CKD. . (CKD) damage (HD Amazon Methods crosssectional cross sectional MSQ (MSQ IIEF5 IIEF (IIEF5 210 21 0 21. tests MannWhitney Mann Whitney KruskalWallis Kruskal Wallis P 0.05. 005 0.05 05 0.05) Results Ninetyeight Ninety eight 51.68 5168 51 68 (51.6 1528 15 28 15.2 evaluated marriedor married or 60.20%, 6020 60.20% , 60 20 (60.20%) 55.10%, 5510 55.10% 55 10 (55.10%) 117 17 1.17 6630 66 30 66.30% p 0.01, 001 0.01 01 0.01) 0.02, 002 0.02 02 0.02) 0.04, 004 0.04 04 0.04) 0.03. 003 0.03 03 0.03) 75 Conclusion (CKD (IIEF 2 00 0.0 51.6 516 (51. 152 15. 602 60.20 (60.20% 551 55.10 (55.10% 11 1.1 663 66.30 7 0. 51. (51 60.2 (60.20 55.1 (55.10 1. 66.3 (5 60. (60.2 55. (55.1 66. ( (60. (55. (60 (55 (6

Abstract Introduction: Hereditary transthyretin amyloidosis (ATTRv) is a severe autosomal dominant systemic disease. It affects the peripheral and autonomic nervous systems, heart, kidneys, and eyes. Amyloid deposition has been demonstrated in the glomerular and tubulointerstitial compartments of the kidney. Therefore, urinary acidification disorders such as renal tubular acidosis (RTA) may be early manifestations of renal involvement in this population. Objective: To evaluate the prevalence of RTA in individuals with ATTRv. Methods: We included symptomatic and asymptomatic individuals with TTR mutation, older than 18 years, GFR >45 mL/min/1.73m2, without systemic metabolic acidosis. Urinary acidification protocol was performed with furosemide and fludrocortisone after 12 h of water deprivation (water deprivation test - WDT) and measurements of urine ammonium ( UNH 4 +) and titratable acidity (UTA). Proximal RTA (pRTA) was diagnosed when FEHCO3>10%. Incomplete form distal RTA (dRTA) was diagnosed if UpH>5.3. Results: We selected 49 individuals with a mean age of 40 (35.5–56.5) years, 63% of which were female, 84% were Caucasian, and mean GFR was 85.5 ± 20.5 mL/min/1.73m2. 94% had the genetic variant Val50Met and 57% were symptomatic. The prevalence of pRTA was 2% and of dRTA was 16.3%. In the subgroup with dRTA, there was no significant increase in excretion of UNH 4 + and UTA. We observed a good correlation between UpH by potentiometry and UpH dipstick. A UpH<5.5 on the dipstick had 100% sensitivity and negative predictive value to exclude dRTA. Conclusion: A high prevalence of RTA was found in individuals with TTR mutations. The UpH dipstick after WDT had good accuracy for screening for dRTA. Further studies are needed to evaluate the impact of early diagnosis and treatment of RTA in this population. Introduction ATTRv (ATTRv disease systems heart kidneys eyes kidney Therefore (RTA population Objective Methods mutation 1 years 45 >4 mLmin173m2 mLminm mL min 73m2 m mL/min/1.73m2 UTA . (UTA) (pRTA FEHCO310 FEHCO FEHCO3 10 FEHCO3>10% (dRTA UpH53 5 3 UpH>5.3 Results 35.5–56.5 355565 35 56 (35.5–56.5 63 female 84 Caucasian 855 85 85. 205 20 20. 94 ValMet Val Met 57 2 163 16 16.3% UpH55 UpH<5. 100 Conclusion mutations > mLmin mLmin173m 73m mL/min/1.73m (UTA FEHCO31 FEHCO3>10 UpH5 UpH>5. 35.5–56. 35556 (35.5–56. 6 8 9 16.3 UpH<5 FEHCO3>1 UpH>5 35.5–56 3555 (35.5–56 16. UpH< FEHCO3> UpH> 35.5–5 355 (35.5–5 35.5– (35.5– 35.5 (35.5 35. (35. (35 (3

Resumo Introdução: A amiloidose hereditária por transtirretina (ATTRv) é uma doença sistêmica autossômica dominante grave. Afeta os sistemas nervoso periférico e autônomo, coração, rins e olhos. A deposição de amiloide foi demonstrada nos compartimentos glomerular e tubulointersticial do rim. Portanto, distúrbios de acidificação urinária, como acidose tubular renal (ATR), podem ser manifestações precoces de envolvimento renal nessa população. Objetivo: Avaliar a prevalência de ATR em indivíduos com ATTRv. Métodos: Incluímos indivíduos sintomáticos e assintomáticos com mutação na TTR, maiores de 18 anos, TFG >45 mL/min/1,73m2, sem acidose metabólica sistêmica. Realizou-se protocolo de acidificação urinária com furosemida e fludrocortisona após 12 horas de privação hídrica (teste de restrição hídrica - TRH) e medições de amônia urinária ( uNH 4 +) e acidez titulável (uTA) na urina. ATR proximal (ATRp) foi diagnosticada quando FEHCO3>10%. ATR distal (ATRd) de forma incompleta foi diagnosticada se pHu>5,3. Resultados: Selecionamos 49 indivíduos com idade média de 40 (35,5–56,5) anos, 63% mulheres, 84% caucasianos e TFG média de 85,5 ± 20,5 mL/min/1,73m2. 94% apresentaram a variante genética Val50Met; 57% eram sintomáticos. A prevalência de ATRp foi 2% e a de ATRd foi 16,3%. No subgrupo com ATRd, não houve aumento significativo na excreção de uNH 4 + e uTA. Observamos uma boa correlação entre pHU por potenciometria e pHU por fita reagente. Um pHU<5,5 na fita reagente apresentou 100% de sensibilidade e valor preditivo negativo para excluir a ATRd. ConclusÃO: Uma alta prevalência de ATR foi encontrada em indivíduos com mutações na TTR. O pHU por fita reagente após TRH teve boa precisão para triagem de ATRd. São necessários mais estudos para avaliar o impacto do diagnóstico e tratamento precoces da ATR nessa população. Introdução ATTRv (ATTRv grave autônomo coração olhos rim Portanto ATR, , (ATR) população Objetivo Métodos TTR 1 anos 45 >4 mLmin173m2 mLminm mL min 73m2 m mL/min/1,73m2 Realizouse Realizou teste uTA (uTA urina (ATRp FEHCO310 FEHCO FEHCO3 10 FEHCO3>10% (ATRd pHu53 pHu 5 3 pHu>5,3 Resultados 35,5–56,5 355565 35 56 (35,5–56,5 63 mulheres 84 855 85 85, 205 20 20, 94 Val50Met ValMet Val Met 57 2 163 16 16,3% pHU55 pHU<5, 100 ConclusÃO (ATR > mLmin mLmin173m 73m mL/min/1,73m FEHCO31 FEHCO3>10 pHu5 pHu>5, 35,5–56, 35556 (35,5–56, 6 8 9 16,3 pHU5 pHU<5 FEHCO3>1 pHu>5 35,5–56 3555 (35,5–56 16, pHU< FEHCO3> pHu> 35,5–5 355 (35,5–5 35,5– (35,5– 35,5 (35,5 35, (35, (35 (3

Resumo: Introdução: A hipertensão arterial (HA) é a principal causa de morte por doenças cardiovasculares. Apesar dos avanços, o percentual de hipertensos não diagnosticados e não tratados é de 58,4%. A avaliação do dano cognitivo na hipertensão concentra-se na prevenção do acidente vascular cerebral, enquanto o dano funcional é ignorado. Esse manejo inadequado pode ser multifatorial. É objetivo fue analisar a opinião dos médicos sobre a relação entre hipertensão arterial e danos cognitivos. Metodologia: Estudo observacional, descritivo, transversal desenvolvido no período entre agosto de 2020 e agosto de 2023. Análise de dados obtidos a partir de questionário autoaplicável, anônimo e voluntário. Revelar informações sobre o perfil profissional, conhecimento sobre a HA, sua ligação com o comprometimento cognitivo (DC), diagnóstico e tratamento. Resultados: Foram incluídos 222 profissionais, 215 (96,8%) concordam com a existência de ligação entre hipertensão e outros fatores de risco cardiovascular na DC e 218 (98,1%) reconhecem ajudar pacientes com risco de sofrer de D.C. A avaliação da DC é realizada em casos selecionados por 132 (59,4%) participantes e 59 (26,7%) a fazem sempre. Dos que realizam avaliação, 103 (54%) utilizam o Mini Exame do Estado Mental (MEEM), 10 (5,2%) utilizam a Avaliação Cognitiva de Montreal (MoCA) e 9 (4,7%) utilizam o Clock Drawing Test. Em relação à diminuição da pressão arterial em pacientes idosos e a ligação com o risco de DC: 54 (24,3%) não reconhecem risco e 65 (29,2%) reconhecem risco moderado-alto. Em referência à implicação do tratamento de doenças cardiovasculares e DC: 217 (97,7%) reconheceram o efeito benéfico. Discussão: Dado o reconhecimento da ligação entre hipertensão e DC, seria de esperar que a DC fosse investigada na grande maioria, no entanto apenas 26,7% sempre a avaliam. Não há consenso sobre o método, sendo o MEEM o mais utilizado, com baixa aplicação do teste MoCA e/ou Clock Drawing Test, sendo estes últimos os que avaliam a função executiva, majoritariamente alterada nos DC vinculados à HA. Embora o tratamento das doenças cardiovasculares seja reconhecido como benéfico em relação à DC, o controle da HA em idosos é considerado arriscado. É feito um diagnóstico de uma situação em que é evidente uma disparidade entre o que se reconhece como saber e o que se afirma fazer. Conclusões: O papel da doença vascular no dano cerebral funcional é reconhecido, considerando-se necessário conhecer o estado cognitivo dos pacientes, porém há baixa aplicação de testes de triagem que avaliam a função executiva. Nesse contexto, evidencia-se uma lacuna entre o conhecimento e a prática médica.

Abstract: Introduction: High blood pressure (HBP) is the leading cause of death from cardiovascular disease. Despite the advances, the percentage of undiagnosed and untreated hypertensive patients is 58.4%. The evaluation of cognitive damage in HBP focuses on preventing stroke, while functional damage is ignored. This inadequate management may be multifactorial. The objective was to analyze the opinions that doctors have about the relationship between high blood pressure and cognitive damage. Methodology: Observational, descriptive, cross-sectional study developed in the period between August 2020 and August 2023. Analysis of data obtained from a self-administered, anonymous and voluntary questionnaire. Revealing information on the professional profile, knowledge of HBP, its link with cognitive impairment (CD), diagnosis and treatment. Results: 222 professionals were included, 215 (96.8%) agree with the existence of a link between HBP and other cardiovascular risk factors in CD, and 218 (98.1%) acknowledge assisting patients at risk of suffering from CD. The CD evaluation is carried out in selected cases by 132 (59.4%) participants and 59 (26.7%) always do it. Of those who perform evaluation, 103 (54%) use the Mini Mental State Examination (MMSE), 10 (5.2%) use the Montreal Cognitive Assessment (MoCA) and 9 (4.7%) use the Clock Drawing Test. Regarding the decrease in blood pressure in elderly patients and the link with risk of CD: 54 (24.3%) do not recognize risk and 65 (29.2%) recognize a moderate-high risk. In reference to the implication of the treatment of cardiovascular disease and CD: 217 (97.7%) recognized a beneficial effect. Discussion: Given the recognition of the link between HBP and CD, it would be expected that CD would be investigated in the vast majority, however only 26.7% always evaluate it. There is no consensus on the method, the MMSE being the most used, with a low application of the MoCA test and/or Clock Drawing Test, the latter being the ones that evaluate executive function, mostly altered in CD linked to HBP. Although the treatment of cardiovascular disease is recognized as beneficial with respect to CD, the control of HBP in older adults is considered risky. A diagnosis is made of a situation where a disparity is evident between what one recognizes as knowing and what one claims to do. Conclusions: The role of vascular disease in functional brain damage is recognized, considering it necessary to know the cognitive status of patients, however there is a low application of screening tests that evaluate executive function. In this context, a gap between medical knowledge and practice is shown.

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Resumen (Objetivo) Plasmodium vivax infecta ampliamente a muchas personas en algunas regiones. La singularidad de formar una etapa latente hace que la malaria vivax pueda ser inducida por una recaída por una infección adicional. En el presente estudio, utilizamos un enfoque de simulación dinámica y acoplamiento molecular ara predecir la posibilidad de que compuestos bioactivos de la familia de plantas Zingiberaceae sean candidatos a fármaco contra la malaria dirigiéndose a la proteína de unión a Duffy de Plasmodium vivax (PvDBP). Se requiere la interacción molecular PvDBP-DARC para mediar el proceso de invasión de merozoitos en los glóbulos rojos. La inhibición de este proceso posiblemente pueda controlar el crecimiento y desarrollo del parásito. (Metodología) El análisis de acoplamiento molecular dio como resultado los dos compuestos principales con el valor de energía de unión más bajo, incluida la 5,7-dihidroxiflavanona (-9,3 kcal/mol) y la pinostrobina (-9,2 kcal/mol). (Resultados) Estos compuestos previstos tienen una interacción más fuerte que la cloroquina como control. Además, los compue potenciales también interactúan con los residuos del sitio de unión de DARC y lo mantienen durante el proceso de simulación de dinámica molecular. De lo contrario, la cloroquina como control no puede retener el 75% de los residuos de unión hacia PvDBP. El estudio de dinámica molecular reveló que los tres complejos tienen una estabilidad relativamente similar. (Conclusiones) Predijimos que los dos compuestos bioactivos tienen potencial como inhibidores de la invasión de merozoitos.

Resumo (Objetivo) O Plasmodium vivax infecta amplamente muitas pessoas em algumas regiões. A singularidade de formar um estágio latente significa que a malária vivax pode ser induzida por recaída devido à infecção adicional. No presente estudo, utilizamos uma abordagem de simulação dinâmica e de acoplamento molecular para prever a possibilidade de compostos bioativos da família de plantas Zingiberaceae como candidatos a medicamentos antimaláricos, visand o aproteína de ligação Duffy do Plasmodium vivax (PvDBP). A interação molecular PvDBP-DARC é necessária para mediar o processo de invasão de merozoítos nos glóbulos vermelhos. A inibição deste processo pode possivelmente controlar o crescimento e desenvolvimento do parasita. (Metodologia) A análise de acoplamento molecular resultou nos dois principais compostos com o menor valor de energia de ligação, incluindo ,7-dihidroxiflavanona (-9,3 kcal/mol) e pinostrobina (-9,2 kcal/mol). (Resultados) Esses compostos previstos têm uma interação mais forte do que a cloroquina como controle. Além disso, os compostos potenciais também interagem e mantêm resíduos do sítio de ligação do DARC durante o processo de simulação de dinâmica molecular. Caso contrário, a cloroquina como controle não pode reter 75% dos resíduos de ligação ao três complexos apresentam estabilidade relativa compostos bioativos têm potencial como inibidor Palavras chave: Medicamentos antimalária; ePvmeesdmsilDBP. ntesa invício;O estudo de dinâmica molecular revelou que os emelhante. (Conclusões) Previmos que os dois asão de merozoítos. Plasmodium vivax; PvDBP; Zingiberáceas.

Abstract (Objective) Plasmodium vivax predominantly infects many people in numerous tropical areas, including Southeast Asia, the Western Pacific, the Americas, and the Eastern Mediterranean. The uniqueness of forming dormant stages can lead to relapse in vivax malaria upon further infection. This study used molecular docking and dynamic simulation to predict potential bioactive compounds from the Zingiberaceae plant family as inhibitors by targeting Plasmodium vivax Duffy Binding Protein (PvDBP). PvDBP-DARC molecular interaction is required to mediate the merozoite invasion process into red blood cells. Inhibiting this process can possibly control parasite growth and development. (Methodology) Molecular docking screening was conducted by using 138 natural compounds from the Zingiberaceae plant family targeting Plasmodium vivax Duffy binding protein (PvDBP). The top two compounds with the lowest binding energy were selected to be analyzed by pharmacokinetics prediction and molecular dynamic (MD) simulation. (Results) Molecular docking screening resulted in the top two compounds with the lowest binding energy value, including 5,7-dihydroxyflavanone (-9.3 kcal/mol) and pinostrobin (-9.2 kcal/mol). These compounds are predicted to have stronger interaction than chloroquine as a control. Furthermore, the potential compounds also interact with DARC binding site residues and maintain them during the molecular dynamic simulation process. Otherwise, chloroquine as a control cannot retain 75% binding residues towards PvDBP. A molecular dynamic study revealed that all three complexes have relatively similar stability. (Conclusions) We predicted that the two bioactive compounds (5,7-dihydroxyflavanone and pinostrobin) have the potential as merozoite invasion inhibitors.

Abstract Introduction/Objective: Research on adult attachment has predominantly been conducted in Anglo-Saxon and European countries, with no comprehensive compilation of contributions from Latin America to date. The objective of the present study was to perform a systematic review of empirical research on adult attachment in Latin America over the past decade. Method: Using the PRISMA methodology, a search was conducted in the Web of Science, Scopus, PsycINFO, and Scielo databases between April and August 2023. Empirical research published in the last 10 years in Spanish, English, and Portuguese, focusing on the study of adult attachment, were included. Some of the exclusion criteria were empirical research with samples of children and adolescents, as well as studies using Latino samples recruited outside of Latin American countries. The risk of bias in the included studies was assessed using The Mixed Methods Appraisal Tool. Results: After the screening and review process, 62 articles were examined. Most of the studies employed quantitative methodologies and were rooted in the tradition of social and personality psychology rather than developmental psychology. The topics addressed are grouped into individual and relational functioning variables, with results consistent with those identified in studies conducted in other regions. Conclusions: The implications of these findings are discussed, and future research directions are proposed. One limitation is the possible omission of relevant unpublished studies or available in other data bases.

Resumen Introducción/objetivo: las investigaciones sobre el apego adulto se han realizado principalmente en países anglosajones o europeos, y hasta el momento no ha habido una recopilación de las contribuciones provenientes de Latinoamérica. El presente estudio tiene como objetivo hacer una revisión sistemática de la investigación empírica sobre el apego adulto en América Latina en la última década. Método: utilizando la metodología PRISMA, se efectuó una búsqueda en las bases de datos Web of Science, Scopus, PsycINFO y Scielo entre abril y agosto de 2023. Se incluyeron investigaciones empíricas publicadas en los últimos 10 años en español, inglés y portugués, centradas en el estudio del apego adulto. Entre los criterios de exclusión considerados se encuentran las investigaciones empíricas con muestras de niños y adolescentes, así como estudios que utilicen muestras latinas reclutadas fuera de los países latinoamericanos. El riesgo de sesgo de los estudios incluidos se evaluó mediante la herramienta The Mixed Methods Appraisal Tool. Resultados: tras el proceso de tamizaje y revisión, se revisaron 62 artículos. La mayoría de los estudios han empleado metodologías cuantitativas, principalmente desde la tradición de la psicología social y de la personalidad, más que de la proveniente de la psicología evolutiva. Los tópicos abordados se agrupan en variables de funcionamiento individual y relacional, con resultados consistentes con los identificados en otras regiones. Conclusiones: se discuten las implicancias de estos hallazgos y se proponen líneas de investigación futura. Una limitación del presente estudio es la posible omisión de investigaciones relevantes no publicadas o disponibles en otras bases de datos.

Abstract Shrimp aquaculture has rapidly expanded in the last decades, representing an important economic activity worldwide; however, infections (bacterial and viral) are one of the biggest challenges in aquaculture activity. Acute Hepatopancreatic Necrosis Disease (AHPND) is a bacterial disease that affects shrimp farm ponds and occurs during the first 30 days after stocking in shrimp ponds, causing mortalities of up to 70 - 100 %. Microbiota has an important role in shrimp aquaculture and disease control. In the present study, pirA and pirB genes were amplified by PCR to confirm AHPND and non-AHPND in shrimp hepatopancreas; samples were sequenced using the MiSeq platform targeting the V3-V4 16S ribosomal (rRNA) hypervariable regions. Results demonstrated a diverse microbiota in the non-AHPND group, mainly composed of phyla Proteobacteria, Firmicutes, and Actinobacteria. Furthermore, Proteobacteria was the dominant phyla in the hepatopancreas infected with AHPND, while Firmicutes tended to decrease. Vibrio was the most abundant at the genus level, prevailing in some genera like Corynebacterium, Weissella, Lactobacillus, Photobacterium, and Geobacillus. Results suggest that AHPND causes dysbiosis in the hepatopancreatic microbiota, and the Firmicutes phylum could require to be restored under such scenario.

Resumen La producción acuícola de camarón se ha expandido rápidamente en las últimas décadas, convirtiéndose en una importante actividad económica a nivel mundial. Sin embargo, algunas infecciones bacterianas y virales son un gran desafío para esta actividad. La Necrosis Hepatopancreática Aguda (AHPND) es una enfermedad bacteriana que afecta a los estanques de granjas camaroneras y se presenta durante los primeros 30 días después de la siembra en estanques de camarones, causando una mortalidad del 70 - 100 %. La microbiota tiene un papel importante en la producción del camarón y el control de enfermedades. En el presente estudio, los genes pirA y pirB fueron amplificados por PCR para confirmar tanto organismos positivos como negativos a AHPND en hepatopáncreas de camarones. Posteriormente se secuenciaron las regiones hipervariables ribosómicas (ARNr) V3-V4 del gen 16S utilizando la plataforma MiSeq. Los resultados demostraron una microbiota diversa en el grupo negativo a AHPND, compuesto principalmente por filos Proteobacteria, Firmicutes y Actinobacteria. Además, Proteobacteria fue el filo dominante en el hepatopáncreas infectado con AHPND, mientras que Firmicutes tendió a disminuir. Vibrio fue el género más abundante, prevaleciendo sobre algunos géneros como Corynebacterium, Weissella, Lactobacillus, Photobacterium y Geobacillus. Los resultados sugieren que la enfermedad de AHPND causa disbiosis en la microbiota hepatopancreática, y el filo Firmicutes podría requerir ser restaurado en dicho escenario.

Abstract Within this academic research article, a bacterium isolated from a marine soil environment near the Mediterranean Sea is observed to possess the potential for producing various fatty acids, particularly n-caproic and oleic acids, as evidenced by FAME profiling. Furthermore, the introduction of glucose into the growth medium enhances the production of caprylic acid rather than caproic acid. The 16S rDNA sequencing suggested that the MZ1 strain belongs to Bacillus subtilis and is closely related to many halophilic species. FAME profiling reveals that the isolated MZ1 is competent in the total production of fatty acids compared to other marine bacterial candidates. Furthermore, the results indicate that MZ1 is efficient in the production of many other fatty acids. This exploration suggests that the marine bacterium Bacillus subtilis MZ1 can be used for fatty acids synthesis, which could be valuable for biodiesel production and other applications.

Resumen Dentro de este artículo de investigación académica, se observa que una bacteria aislada de un entorno de suelo marino cerca del mar Mediterráneo posee el potencial de producir diversos ácidos grasos, especialmente los ácidos n-caproico y oleico, según lo evidencia el perfil FAME. Además, la introducción de glucosa en el medio de crecimiento potencia la producción de ácido caprílico en lugar de ácido caproico. La secuenciación del 16S rDNA sugiere que la cepa MZ1 pertenece a Bacillus subtilis y está estrechamente relacionada con muchas especies halófilas. El perfil FAME revela que la cepa MZ1 aislada es competente en la producción total de ácidos grasos en comparación con otros candidatos bacterianos marinos. Además, los resultados indican que MZ1 es eficiente en la producción de muchos otros ácidos grasos. Esta exploración sugiere que la bacteria marina Bacillus subtilis MZ1 puede ser utilizada para la síntesis de ácidos grasos, lo cual podría ser valioso para la producción de biodiesel y otras aplicaciones.