ABSTRACT Ewing sarcoma (ES) is a highly malignant and aggressive small round-cell tumor originating from primitive neuroepithelium and mesenchymal stem cells. It is usually seen in children and adolescents with a male predilection and a preponderance to occur in long bones. Although skeletal/soft tissue ES is encountered in clinical practice, primary ES of the genital tract, particularly bilateral primary ovarian ES, is highly uncommon, with only a handful of cases reported worldwide. Ovarian ES is occasionally reported to involve para-aortic and pelvic lymph nodes in advanced stages. Still, cervical lymph node metastasis from ovarian ES is an infrequent clinical occurrence and, when present, indicates a worse prognosis. Here, we present an intriguing case of bilateral peripheral primary ovarian ES in an adult female, recurring as metastasis in the left submandibular lymph node. This case underlines the importance of keeping metastasis from ES as a possible differential while diagnosing metastatic small round cell tumors in peripheral lymph nodes. It also highlights the usefulness of a minimally invasive diagnostic modality of fine needle aspiration cytology and cell block preparation with applied ancillary techniques of immunohistochemistry and confirmatory molecular testing by fluorescence in-situ hybridization (FISH), for an accurate and quick diagnosis of such entities. The cytological diagnosis of our patient helped in the prompt and early initiation of chemotherapy without requiring any invasive procedure. (ES roundcell cells bones skeletalsoft skeletal soft practice tract uncommon worldwide paraaortic para aortic stages Still prognosis Here female insitu situ FISH, FISH , (FISH) entities procedure (FISH

SUMMARY Acute suppurative thyroiditis is an uncommon disorder caused by a bacterial infection, usually presenting with normal thyroid function. It is a serious condition that requires a prompt diagnosis and treatment with antibiotics and supportive measures. A 62 years-old female presented with a painful cervical induration and odynophagia a week after a fish bone had been removed from her pharynx. She was febrile, and tachycardic and, on physical examination, a painful thyroid mass was detected. High inflammatory parameters and thyrotoxicosis were confirmed: thyroid stimulating hormone (TSH) < 0.01 mIU/L (normal range [NR] 0.27-4.2); free thyroxine (FT4) 3.86 ng/dL (NR 0.9-1.7) and anti-TSH receptor antibodies (TRABs) 5.3 U/L (NR < 1.5). Thyroid scintigraphy showed a diffuse uptake of the thyroid parenchyma suggesting Graves disease. Cervical ultrasonography revealed an abscess of the left thyroid lobe of 36 × 36 mm and fine needle aspiration biopsy (FNAB) with partial drainage was performed. Staphylococcus aureus and Streptococcus viridans were isolated, and directed antibiotic therapy was started. Clinical improvement was observed as well as a decrease of inflammatory parameters and the patient was discharged after 9 days of hospitalization. Eighteen days after discharge, thiamazole was initiated due to persistent thyrotoxicosis. Complete resolution of the abscess was documented within 6 months and the patient became euthyroid under thiamazole one year after initial presentation. To our knowledge, this is the third case reporting an association between acute thyroiditis and Graves disease. Furthermore, this is the first case detailing the simultaneous diagnosis of acute suppurative thyroiditis caused by a foreign body and Graves disease. infection function measures yearsold years old pharynx febrile examination detected confirmed TSH (TSH 001 0 01 0.0 mIUL mIU L NR [NR 0.274.2 02742 0.27 4.2 27 4 2 0.27-4.2) FT4 FT (FT4 386 3 86 3.8 ngdL ng dL 0.91.7 0917 0.9 1.7 1 7 0.9-1.7 antiTSH anti TRABs (TRABs 53 5 5. UL U 1.5. 15 1.5 . 1.5) disease FNAB (FNAB performed isolated started hospitalization discharge presentation knowledge Furthermore 00 0. 274 0.274. 0274 027 0.2 42 4. 0.27-4.2 (FT 38 8 3. 91 0.91. 091 09 17 1. 0.9-1. 0.274 02 0.27-4. 0.91 0.9-1 0.27-4 0.9- 0.27-

ResumenAlgunas especies de peces marinos tienen complejas relaciones con los ecosistemas costeros durante sus periodos reproductivos, tal es el caso del pez aguja Tylosurus pacificus, que conforma agregaciones de desove en playas de grava en el Parque Nacional Natural Utría. Se describe la actividad pesquera y algunos aspectos de la biología reproductiva de T. pacificus y se proponen algunas medidas para mitigar el impacto sobre este evento y los procesos ecológicos asociados. Se realizó un monitoreo pesquero entre abril 2008 y febrero 2009 en el Parque Nacional Natural Utría (PNNU), Pacífico colombiano. Se analizaron los volúmenes de captura, estructura por talla, proporción de sexos y fecundidad (n= 84). La longitud total osciló entre 60.5 cm y 104 cm con una media y desviación estándar de 85.35 ± 9.09 cm. La fecundidad promedio fue 189 685.56 ovocitos por gónada, con una moda de 8 mm de diámetro y un desarrollo gonadal asincrónico. La especie desova en forma colectiva en la playa cuando la marea alcanza el nivel más alto durante la luna llena y nueva, generalmente al anochecer. Pescadores que habitan los pueblos cercanos aprovechan este recurso, durante la agregación reproductiva de la especie. Es importante ampliar el estudio de la reproducción de T. pacificus y los procesos ecológicos asociados a su desove para poder dar un aprovechamiento adecuado y garantizar la sostenibilidad de su pesquería a largo plazo.

Abstract:Some species of marine fish have complex relationships with coastal ecosystems during their reproductive periods, as the needle fish Tylosurus pacificus that forms spawning aggregations in gravel beaches in Utría Park. We described fishery and some aspects of the reproductive biology of T. pacificus and proposed some strategies to mitigate the impact of this event and associated ecological processes. Fisheries monitoring were conducted between April 2008 and February 2009 in the Utría National Park, Colombian Pacific. Catch volumes, length structure, sex ratio and fecundity (n= 84) were analyzed. The total length ranged among 60.5 and 104 cm with a mean and standard deviation of 85.35 ± 9.09 cm. The average fertility of oocytes per gonad was 189 685.56, with a mode of 8 mm diameter and an asynchronous gonadal development. This species spawns collectively on the beach when the tide reaches the highest level during full and new moon, usually in the evening. Fishermen of a near town take advantage of the spawning aggregation of this species. The reproduction study of T. pacificus and ecological processes associated with their spawning, should be expanded to give adequate use, and ensure the sustainability of their fishery over the long term. Rev. Biol. Trop. 65 (1): 77-87. Epub 2017 March 01.

O jundiá é espécie de interesse econômico para região sul do Rio Grande do Sul, pois adapta-se a diferentes ambientes e dietas artificiais, apresentando fácil manejo e boa aceitação comercial. O bom estado nutricional é fator determinante no desenvolvimento dos peixes. A formação das células sangüíneas é dependente da manipulação da dieta. O objetivo deste trabalho foi avaliar o efeito da dieta com diferentes níveis de proteína sobre os parâmetros eritrocitários do jundiá. Foram utilizados 90 peixes, com comprimento padrão médio de 20,0±1,2cm e peso médio de 131,4±25,9g. Os animais foram distribuídos em três caixas de 1.000L, contendo três cestos (80L) cada, com três níveis de proteína bruta (30, 40 e 50%). Os animais foram amostrados a cada duas semanas, durante 45 dias. Após serem anestesiados, foi realizada a coleta de sangue através de punção caudal, utilizando-se agulha e seringa que contêm EDTA a 10%. As análises sangüíneas correspondentes à série eritrocitária foram realizadas através de contador automatizado. O nível protéico da dieta influencia os parâmetros eritrocitários, em especial o número total de eritrócitos, a taxa de hemoglobina e o hematócrito, sendo que o nível de 50% de PB foi o que mais estimulou a eritropoiese.

Silver catfish is a species of economic interest for the southern Rio Grande do Sul region, Brazil, being well adapted to different environments and artificial diets, showing easy management and good commercial acceptance. Good nutritional status is a determinant factor in fish development. Difference in formation of blood cells is dependent of diet manipulation. The objective of this study was to evaluate the effect of a diet with different protein levels on erythrocytic parameters of silver catfish. Ninety (90) fish were used with an average standard length of 20.01±1.28cm and average weight of 131.4±25.9g distributed in three 1,000L tanks, containing three baskets (80L) each, with three levels of crude protein (30, 40 and 50%). Animals were sampled every two weeks during 45 days. After anesthetization blood was collected through caudal puncture using needle and syringe containing EDTA at 10%. Blood analyses corresponding to the erythrocyte series were performed through automatic counter. Proteic level of diet influences erythrocytic parameters, especially the total number of erythrocytes, hemoglobin rate and hematocrit. The level of 50% CP showed best stimulation of erythropoiesis.

O presente trabalho descreve um procedimento simples para reibridização in situ fluorescente em núcleos interfásicos de amostras frescas de tumores de próstata obtidas a partir de biopsias aspirativas. Sondas <img src="http:/img/fbpe/gmb/v22n2/s_alpha.gif" align="absmiddle">-centroméricas marcadas com digoxigenina foram hibridizadas em núcleos anteriormente avaliados com diferentes sondas marcadas com biotina. Esse método permitiu a análise da mesma célula com diferentes seqüências de DNA centroméricas, especialmente em casos com um número limitado de lâminas disponíveis.

We present a simple procedure for interphase fluorescence in situ rehybridization (FISH). This procedure was used to evaluate fresh prostate tumor from needle biopsy specimens. Digoxigenin-labeled DNA probes were hybridized onto nuclei which had been previously investigated by FISH using biotin-labeled DNA probes. This method makes it possible to reanalyze the same nuclei with different centromeric DNA probes and is useful in cases where a limited number of slides are available.