Bisphenol A (BPA), an endocrine disruptor used in different commercial polymers, is a persistent pollutant commonly found in effluents. Conventional wastewater treatment processes have low chemical removal efficiency and are expensive. This work evaluated BPA removal using laccase enzyme produced from white rot fungus Pleurotus ostreatus. For the enzymatic production, residues of acai berry and pineapple were used as laccase inducers. The use of natural inducers led to a high enzyme production (1139 and 1031 U mL−1 to acai and pineapple, respectively). Bisphenol A was removed to a concentration lower than LOD (limit of detection) after 4 h. The degradation mechanism of BPA occurred by oxidation of methyl to hydroxymethyl group in a propane portion, with breakdown of the aromatic ring. The developed technology brought to the scene a new green, viable methodology, using vegetable waste, adding value to these residues and bringing an alternative to the BPA treatment. BPA, , (BPA) polymers effluents expensive ostreatus 1139 (113 103 mL1 mL 1 mL− respectively. respectively . respectively) limit detection h portion ring green methodology waste (BPA 113 (11 10 11 (1 (

RESUMO A densidade de plantas e o espaçamento correto são fatores fundamentais para alcançar produtividade rentável e qualidade dos frutos em culturas agrícolas. Este estudo teve como objetivo avaliar a produtividade do tomateiro de crescimento determinado e as características físico-químicas dos frutos, em diferentes arranjos de plantio e densidades de plantas. Foram realizados dois experimentos, sendo que no primeiro experimento e no segundo experimento foram utilizados os híbridos CVR-2909 (CVR Plant Breeding) e HMX 7885 (Agristar), respectivamente. Os experimentos foram conduzidos em delineamento de blocos casualizados, com quatro repetições. Foram avaliados dez tratamentos em esquema fatorial duplo (2 × 5). No primeiro fator, foram avaliados dois arranjos de plantio: linhas simples e linhas duplas. No segundo fator, foram avaliadas cinco densidades de plantas: 15, 20, 25, 30 e 35 mil plantas ha-1. Para o híbrido CVR-2909, o cultivo em linhas simples proporcionou maior produtividade, já as maiores densidades de plantas resultaram em menor produção e número de frutos por planta. Para o híbrido HMX-7885, maiores densidades de plantas proporcionaram maior produtividade, porém com menor produção por planta, massa média e número de frutos por planta. Para esse híbrido o arranjo de plantio proporcionou maior taxa de frutos verdes. agrícolas físicoquímicas físico químicas CVR2909 CVR 2909 CVR-290 Breeding 788 Agristar, Agristar , (Agristar) respectivamente casualizados repetições 2 ( 5. 5 . 5) fator duplas 15 20 25 3 ha1. ha1 ha 1. 1 ha-1 CVR2909, 2909, planta HMX7885, HMX7885 7885, HMX-7885 verdes CVR290 290 CVR-29 78 (Agristar ha- HMX788 HMX-788 CVR29 29 CVR-2 7 HMX78 HMX-78 CVR2 CVR- HMX7 HMX-7 HMX-

ABSTRACT Plant density and correct row spacing are fundamental factors for achieving profitable yield and fruit quality in crops. This study aimed to evaluate the yield of tomato plants of determinate growth and the physicochemical characteristics of the fruits in different planting arrangements and plant densities. Two experiments were conducted using the hybrids CVR-2909 (CVR Plant Breeding) and HMX 7885 (Agristar) in the first and second experiments, respectively. The experiments were conducted in a randomized block design with four replicates. Ten treatments were evaluated in a double factorial scheme (2 × 5). Two planting arrangements were evaluated in the first factor: single and double rows. The second factor comprised five plant densities: 15, 20, 25, 30, and 35 thousand plants ha-1. For the CVR-2909 hybrid, single-row cultivation resulted in the highest yield, while higher planting densities gave the lowest yield and number of fruits per plant. For the HMX-7885 hybrid, it was found that higher densities gave higher yields but caused a decreasing effect on fruit production per plant, average fruit mass, and number of fruits per plant. For this hybrid, the planting arrangement provided a higher rate of green fruits. crops CVR2909 CVR 2909 CVR-290 Breeding 788 Agristar (Agristar respectively replicates 2 ( 5. 5 . 5) rows 15 20 25 30 3 ha1. ha1 ha 1. 1 ha-1 hybrid singlerow HMX7885 HMX-788 mass CVR290 290 CVR-29 78 ha- HMX788 HMX-78 CVR29 29 CVR-2 7 HMX78 HMX-7 CVR2 CVR- HMX7 HMX-

RESUMO: Os estudos visando o melhoramento de lúpulo (Humulus lupulus) no Brasil são recentes e buscam estabelecer a autossuficiência da produção. O conhecimento da variabilidade dos genótipos disponíveis no país é de fundamental importância para o desenvolvimento de um programa de melhoramento de lúpulo. O objetivo do trabalho foi identificar genitores de lúpulo com potencial agronômico e cervejeiro para o Planalto Catarinense, Brasil. Foi utilizado o delineamento em blocos casualizados em esquema fatorial 3x4 com 12 tratamentos: Fator 1: três anos de cultivo (2019, 2020 e 2021) e combinado com o fator 2: quatro genótipos (Cascade, Chinook, Columbus e Hallertau). Os caracteres avaliados foram a massa fresca de plantas (MFP, g), massa verde de cones (MTV, g), a massa seca de cones (MSC, g), os teores de alfa-ácidos (ALFA, %) e a produção (PROD, g per planta). O genótipo Hallertauer demonstrou melhor desempenho e comportamento diferenciado para os caracteres de massa fresca de plantas, massa seca de cones e produção, quando comparado aos demais. Assim, a partir destes resultados observou-se que, existe variação disponível ao melhoramento genético do lúpulo para esses caracteres, fator que permitirá obter ganhos produtivos no desenvolvimento de novas cultivares, a partir deste genótipo promissor. RESUMO Humulus lupulus Catarinense x 3x 1 tratamentos 2019, 2019 (2019 202 2021 2 Cascade, Cascade (Cascade Chinook Hallertau. Hallertau . Hallertau) MFP, MFP (MFP g, , g) MTV, MTV (MTV MSC, MSC (MSC alfaácidos alfa ácidos ALFA, ALFA (ALFA % PROD, PROD (PROD planta. planta planta) demais Assim observouse observou se cultivares promissor 201 (201 20 (20 (2 (

ABSTRACT: Studies on the improvement of hops (Humulus lupulus) in Brazil are recent and seek to establish self-sufficiency in production. Knowledge of the variability of genotypes available in the country is of fundamental importance for the development of a hop improvement program. The objective of the research was to characterize and identify hop parents with agronomic and brewing potential for the Planalto Catarinense region, Brazil. The randomized block design arranged in a 3x4 factorial scheme was used with 12 treatments: (Factor 1: three years of cultivation (2019, 2020 and 2021) and combined with factor: four genotypes (Cascade, Chinook, Columbus and Hallertau). The characters evaluated were fresh mass of plants (MFP, g), green mass of cones (MTV, g), dry mass of cones (MSC, g), alpha-acid content (ALFA, %) and production (PROD, g per plant). The Hallertauer genotype showed better performance and differentiated behavior for the characters of fresh mass of plants, dry mass of cones and production, when compared to the others. Thus, from these results it was observed that there is variation available for the genetic improvement of hops for these characters, a factor that will allow obtaining productive gains in the development of new cultivars, based on this promising genotype. ABSTRACT Humulus lupulus selfsufficiency self sufficiency program region x 3x 1 treatments Factor 2019, 2019 (2019 202 2021 Cascade, Cascade (Cascade Chinook Hallertau. Hallertau . Hallertau) MFP, MFP (MFP g, , g) MTV, MTV (MTV MSC, MSC (MSC alphaacid alpha acid ALFA, ALFA (ALFA % PROD, PROD (PROD plant. plant plant) others Thus cultivars 201 (201 20 (20 2 (2 (

RESUMO: Os padrões paisagísticos em zonas montanhosas com rápido desenvolvimento económico são fortemente afetados por múltiplas atividades humanas. No entanto, as mudanças nas alterações da paisagem nas áreas montanhosas cársticas atribuídas a atividades humanas complexas permanecem obscuras. A investigação sobre o grau de alteração da paisagem em zonas montanhosas sob a influência das atividades humanas é de grande importância para a conservação e gestão científica das paisagens montanhosas. Selecionamos zonas rurais com quatro tipos típicos de acidentes geográficos de pequena escala em áreas montanhosas como objetos de pesquisa e analisamos a resposta das mudanças na perturbação da paisagem rural à atividade humana nessas áreas. Nossos resultados revelam que o grau de alteração da paisagem e a atividade humana influenciam o aumento do índice na maioria das áreas montanhosas. As mudanças na atividade humana e nas perturbações paisagísticas são geralmente observadas na região de baixo relevo do terreno. A utilização intensiva do solo associada ao rápido desenvolvimento econômico tem um sério impacto negativo no grau de perturbação da paisagem, enquanto os projetos de restauração ecológica exercem um impacto positivo. Acelerar a implementação dos projetos para área “verde” e regular a expansão não regulamentada da produção e da terra viva são medidas cruciais para a gestão ecológica em áreas montanhosas cársticas. As futuras estratégias de gestão ecológica deverão centrar-se em áreas de terreno relativamente planas. Nossas descobertas contribuem para a gestão da paisagem e a regulação da atividade humana de diferentes tipos de relevo de pequena escala em áreas montanhosas cársticas. RESUMO entanto obscuras positivo verde “verde centrarse centrar se planas

ABSTRACT: Landscape patterns in mountainous areas with rapid economic development are strongly affected by multiple human activities. However, the changes in landscape disturbances across karst mountainous areas attributed to complex human activities remain unclear. Research on the degree of landscape disturbance in mountainous areas under the influence of human activities is of great significance to the conservation and scientific management of mountainous landscapes. We selected rural zones with four typical small-scale landform types in karst mountainous areas as the research objects and analyzed the response of changes in rural landscape disturbance to human activity in these areas. Our results revealed that the landscape disturbance degree and human activity influence index increase in the majority of karst mountainous areas. The changes in human activity and landscape disturbances are generally observed in the low terrain relief region. High-intensity land use associated with rapid economic development has a serious negative impact on the degree of landscape disturbance, while ecological restoration projects exert a positive impact. Accelerating the implementation of the grain for green project and regulating the unregulated expansion of production and living land are crucial measures for the ecological management in karst mountainous areas. Future ecological management strategies should focus on relatively flat terrain areas. Our findings contributed to the landscape management and human activity regulation of different small-scale landform types in karst mountainous areas. ABSTRACT However unclear landscapes smallscale small scale region Highintensity High intensity

ABSTRACT Proper waste management is a major worldwide concern; waste is disposed of directly into the environment, affecting water, soil and air quality. The development and production of green energy sources is strongly encouraged in modern society. The aim of this study, in accordance with Sustainable Development Goals (SDGs) 1, 2 and 7 of the 2030 Agenda of the UN, is to combine the correct disposal of animal waste with the generation of energy to mitigate the energy insecurity of families in an extremely vulnerable semi-arid region in the north-east of the country using biodigesters. To this end, a conceptual proposal was drawn up for a low-cost, easy-to-maintain and easy-to-use biodigester. A small-scale prototype was used to assess its operation by evaluating the main factors that affect biogas production (pH, temperature and the biomass properties: total solids, fixed solids, volatile solids, concentration of volatile fatty acids and C/N ratio). Evaluation of the test prototype gave satisfactory results that corroborate other studies and make it possible to propose an economically viable and efficient full-scale biodigester for biogas production. concern environment water quality society study SDGs (SDGs 1 203 UN semiarid semi arid northeast north east biodigesters end lowcost, lowcost low cost, cost low-cost easytomaintain easy maintain easytouse use smallscale small scale pH, pH (pH properties solids CN C N ratio. ratio . ratio) fullscale full 20

RESUMO O aumento da fertilidade do solo no cultivo de hortaliças apresenta desafios, notadamente usando adubo verde de espécies espontâneas do bioma Caatinga, como a flor-de-seda (Calotropis procera [Ait.] R. Br.). Este estudo teve como objetivo avaliar e otimizar as eficiências física e econômica da rúcula em monocultivo, influenciadas por quantidades variáveis de biomassa de flor-de-seda. Um delineamento em blocos casualizados com sete tratamentos e cinco repetições foi utilizado. Os tratamentos consistiram de diferentes quantidades de biomassa de C. procera (20, 40, 60, 80 e 100 t ha-1 em base seca), juntamente com dois tratamentos adicionais em cada bloco: um controle (sem fertilização) e um com fertilização mineral. A cultivar de rúcula "Cultivada" foi adubada para máxima eficiência produtiva usando 63,31 t ha-1 de biomassa seca de C. procera, rendendo 8,45 t ha-1. A maior eficiência agroeconômica otimizada, refletindo uma renda líquida de R$ 111.007,64 por hectare, foi alcançada com 59,26 t ha-1 de biomassa seca de C. procera. A taxa de retorno foi de R$ 4,65 para cada real investido, com um índice de rentabilidade de 77,38%. Assim, utilizar biomassa de C. procera como adubo verde é uma tecnologia viável para produtores de rúcula em regiões semiáridas. desafios Caatinga flordeseda flor seda Calotropis Ait. Ait [Ait. R Br.. Br Br. . Br.) monocultivo flordeseda. seda. utilizado C 20, 20 (20 40 60 8 10 ha1 ha 1 ha- seca, , seca) bloco sem mineral Cultivada "Cultivada 6331 63 31 63,3 845 45 8,4 ha1. 1. otimizada 11100764 111 007 64 111.007,6 hectare 5926 59 26 59,2 465 4 65 4,6 investido 7738 77 38 77,38% Assim semiáridas [Ait 2 (2 6 633 3 63, 84 8, 1110076 11 00 111.007, 592 5 59, 46 4, 773 7 77,38 ( 111007 0 111.007 77,3 11100 111.00 77, 1110 111.0 111.

ABSTRACT Enhancing soil fertility in vegetable cultivation presents challenges, notably using green manure from spontaneous species in the Caatinga biome, such as roostertree (Calotropis procera [Ait.] R. Br.). This study aimed to evaluate and optimize the physical and economic efficiencies of monocropped arugula, as influenced by varying amounts of roostertree biomass. We employed a randomized block design with seven treatments and five replications. Treatments involved different quantities of C. procera biomass (20, 40, 60, 80, and 100 t ha-1 on a dry basis), along with two additional treatments in each block: a control (no fertilization) and one with mineral fertilization. The "Cultivada" arugula cultivar was fertilized for maximum productive efficiency using 63.31 t ha-1 of C. procera dry biomass, yielding 8.45 t ha-1. The highest optimized agroeconomic efficiency, reflecting a net income of BRL 111,007.64 per hectare, was achieved with 59.26 t ha-1 of C. procera dry biomass. The return rate was BRL 4.65 for every real investment, with a profitability index of 77.38%. Thus, using C. procera biomass as green manure is a viable technology for arugula producers in semi -arid regions. challenges biome Calotropis Ait. Ait [Ait. R Br.. Br Br. . Br.) replications C 20, 20 (20 40 60 80 10 ha1 ha 1 ha- basis, basis , basis) no fertilization Cultivada "Cultivada 6331 63 31 63.3 845 8 45 8.4 ha1. 1. 11100764 111 007 64 111,007.6 hectare 5926 59 26 59.2 465 4 65 4.6 investment 7738 77 38 77.38% Thus arid regions [Ait 2 (2 6 633 3 63. 84 8. 1110076 11 00 111,007. 592 5 59. 46 4. 773 7 77.38 ( 111007 0 111,007 77.3 11100 111,00 77. 1110 111,0 111,

Summary: Currently, agricultural activity is constantly threatened by the effects of climate change and radical expression of environmental factors as irregular rainfall distribution responsible for water availability and deficit. These factors generate water stress in productive systems, whose negative effect is reflected on morphological, physiological and biochemical aspects of vegetative growth. The use of biostimulants arises as a strategy to counteract this impact, improving crop efficiency in the use of resources and generating responses, as metabolite production, among other substances that mitigate the effects of oxidative stress. In this context, waxes, hydrogen peroxide (H2O2), salicylic acid (SA) and ALGAENZIMSMR were used on raspberry plants subjected to moderate water stress (-0. 8 MPa) to evaluate the fruit response in aspects of vegetative growth, physiological and biochemical quality. For the establishment, a completely randomized design was used with 5 treatments: Green Cover as 5 mL L-1, H2O2 10-4 M, salicylic acid 0.27 mM, ALGAENZIMSMR 7.5 mL L-1 and the control with 50% recovery of the transpired volume as well as water potential similar to the rest of plants and 4 replicates. The results showed that waxes, SA and algal extract increased the photosynthetic rate; the four treatments increased root length; waxes increased root height; and H2O2 increased root biomass. Compared to the control, higher levels of vitamin C and anthocyanins were produced in fruits - a very important aspect due to the functionality of these compounds in human health. Therefore, the use of these biostimulants are a very useful tool for crop management in environments with water restriction.

Resumen: En la actualidad la actividad agrícola se ve amenazada constantemente por los efectos del cambio climático y la expresión radical de los factores ambientales, como la irregularidad en la distribución pluvial responsable de la disponibilidad del agua y déficit, que genera estrés hídrico en los sistemas productivos, cuyo efecto negativo se refleja en aspectos de crecimiento vegetativo, morfológicos, fisiológicos y bioquímicos. El uso de bioestimulantes surge como estrategia para contrarrestar este impacto, mejorando la eficiencia de los cultivos en el uso de los recursos y generar respuestas, como la producción de metabolitos entre otras sustancias, que mitigan los efectos del estrés oxidativo. Bajo este contexto se planteó el uso de ceras, peróxido de hidrogeno (H2O2), ácido salicílico (AS) y algaenzims, sobre plantas de frambuesa sometidas a estrés hídrico moderado (-0.8 MPa) con el objetivo de evaluar la respuesta en aspectos de crecimiento vegetativo, fisiológicos y calidad bioquímica de frutos, para el establecimiento se empleó un diseño completamente al azar con 5 tratamientos: greencover as® 5 mL L-1, H2O2 10-4 M, ácido salicílico 0.27 mM, algaenzims® 7.5 mL L-1 y el control con recuperación del 50% del volumen transpirado y con potencial hídrico similar al resto de plantas y 4 repeticiones. Como resultados se obtuvo que las ceras, AS y el extracto de algas incrementaron la tasa fotosintética, los cuatro tratamientos aumentaron la longitud de raíces, las ceras la altura, y el H2O2 la biomasa de raíz. Por otro lado, en comparación con el control, se produjeron mayores niveles de vitamina C y antocianinas en frutos, un aspecto muy importante por la funcionalidad de estos compuestos en la salud humana. Por lo tanto, el uso de estos bioestimulantes se puede visualizar como una herramienta de gran utilidad para el manejo de cultivos en ambientes con restricción de agua.

Summary: The fraction of vegetation cover (FVC) and the Leaf Area Index (LAI) are biophysical variables closely related to the evapotranspiration rate of crops and their biomass production. Despite their importance, they are usually not measured directly due to their time-consuming and costly nature; however, they can be estimated on a large scale and near-real-time using spectral information captured in satellite images, although this requires prior validation at the field level. Simple linear regression models were developed and validated using 13 vegetation indices (VI) related to LAI and FCV in alfalfa (Medicago sativa). Three spectral information sources were used: (i) reflectance to derive the VI; (ii) digital photographs processed with the Canopeo application to estimate FVC; and (iii) direct LAI measurements using a ceptometer. Six field samples were taken between March and June, 2020 in four alfalfa plots located in Palmar de Bravo, Puebla, Mexico. To evaluate each VI, the determination coefficient (R2) and the root mean square error (RMSE) were used. We found that the most suitable VI for estimating FVC was the VIgreen (Green Vegetation Index, R2 =0.987 and RMSE = 0.093). For the LAI, the VI that performed best was the NDVI (Normalized Difference Vegetation Index, R2 = 0.935 and RMSE = 0.746). The results showed the suitability and practical utility of spectral information for estimating biophysical variables in alfalfa cultivation and their monitoring.

Resumen: La fracción de cobertura vegetal (FCV) y el índice de área foliar (IAF) son variables biofísicas relacionadas estrechamente con la tasa de evapotranspiración de los cultivos y su producción de biomasa. A pesar de su importancia, usualmente no se miden de manera directa debido al tiempo invertido y costo; sin embargo, se pueden estimar a gran escala y en tiempo casi real mediante información espectral captada en imágenes de satélite, pero esto requiere de su validación a nivel de campo. Se desarrollaron y validaron modelos de regresión lineal simple utilizando 13 índices de vegetación (IV) relacionados con el IAF y la FCV en alfalfa (Medicago sativa). Se usaron tres fuentes de información espectral: (i) La reflectancia para derivar los IV; (ii) fotografías digitales procesadas con Canopeo para estimar la FCV; y (iii) mediciones del IAF utilizando un ceptómetro. Se realizaron seis muestreos de campo entre marzo y junio de 2020, en cuatro parcelas de alfalfa situadas en Palmar de Bravo, Puebla, México. Para evaluar cada IV, se utilizó el coeficiente de determinación (R2) y la raíz del cuadrado medio del error (RCME). Se encontró que el IV más apropiado para estimar la FCV fue el VIgreen (Green Vegetation Index, R2 = 0.987 y RCME = 0.093). En el caso del IAF, el IV que presentó el mejor desempeño fue el Normalized Difference Vegetation Index (NDVI), R2 = 0.935 y RCME = 0.746). Los resultados mostraron la aptitud y utilidad práctica de la información espectral para estimar las variables biofísicas en el cultivo de alfalfa y su monitoreo.

Resumen Introducción. En agricultura orgánica, se utilizan abonos verdes y biocontroladores como métodos de fertilización y control de plagas. Sin embargo, la aplicación independiente de estos incrementa los costos de producción. Se propone una presentación peletizada que permita la aplicación conjunta de ambos insumos. Objetivo. Evaluar el efecto de Mucuna pruriens (L.) DC. peletizada e inoculada con Trichoderma harzianum Rifai en el aporte nutricional, el rendimiento agrícola y la regulación de Fusarium oxysporum f. sp. lycopersici en tomate (Solanum lycopersicum L.). Materiales y métodos. El experimento se ejecutó el año 2022 en Tablón del Guarco, Cartago, Costa Rica, bajo un diseño de bloques completos al azar con siete tratamientos y siete repeticiones. Se aplicaron dos formulaciones (A y B) de biopélet (M. pruriens + zeolita + T. harzianum) en tres dosificaciones (15, 30, 50 g/planta). La adición de T. harzianum se efectuó a razón de 5 g por cada kilo del biopélet. Se realizaron aplicaciones cada 15 días por tratamiento para un total de ocho aplicaciones. Se inoculó F. oxysporum (100 mL/planta con una concentración de 1 x 103 UFC) a la siembra. Resultados. La aplicación del biopélet mostró un rendimiento y peso de frutos de primera calidad similar al testigo comercial. En frutos de segunda y tercera calidad, no hubo diferencias significativas entre tratamientos. La eficiencia en el control de F. oxysporum alcanzó el 97,6 % con biopélet. Esta enmienda aumentó los niveles de Carbono, Nitrógeno y materia orgánica en el suelo e influenció el contenido nutricional en el follaje a los 60 días después de la siembra. Conclusiones. El biopélet tiene la capacidad de realizar aportes químicos y orgánicos al suelo, por lo cual mejora la nutrición del cultivo del tomate. Los resultados sugieren además que es una alternativa de biocontrol contra F. oxysporum y compatible con T. harzianum.

Abstract Introduction. In organic agriculture, green manures and biocontrols are used as methods for fertilization and pest control. However, their independent application of these inputs increases production costs. A pelletized formulation is proposed to allow simultaneous application of both inputs. Objective. To evaluate the effect of pelletized Mucuna pruriens (L.) DC. inoculated with Trichoderma harzianum Rifai on nutritional intake, agricultural yield, and regulation of Fusarium oxysporum f. sp. lycopersici in tomato (Solanum lycopersicum L.). Materials and methods. The experiment was carried out in 2022 in Tablón del Guarco, Cartago, Costa Rica, using a randomized complete block design with seven treatments and seven repetitions. Two formulations (A and B) of biopellet (M. pruriens + zeolite + T. harzianum) were applied at three dosages (15, 30, 50 g/plant). T. harzianum was added at a rate of 5 g/kg of biopellet. Applications of each treatment were conducted every 15 days for a total of eight applications. At the time of planting, F. oxysporum was inoculated (100 mL/plant with a concentration of 1 x 103 CFU). Results. The application of biopellet achieved a yield and weight of high-quality fruits similar to the commercial control. There were no significant differences among treatments for second and third-quality fruits. The technical efficiency in controlling F. oxysporum reached 97.6 % with biopellet. This amendment increased the levels of Carbon (C), Nitrogen (N), and organic matter in the soil and affected the nutritional content of the plant foliage in the first 60 days after sowing. Conclusions. The biopellet has the capacity to make chemical and organic contributions to the soil, which improves the nutrition of the tomato crop. It could be also a biocontrol alternative against F. oxysporum and compatible with T. harzianum.

Resumen Introducción. El “moho verde” causado por Penicillium digitatum es uno de los problemas fitosanitarios que limita la producción en los cítricos. Para su control se utilizan fungicidas sintéticos, que han perdido eficacia, debido a la resistencia generada por el hongo. Una alternativa, es el uso de hongos endófitos con acción biocontroladora. Objetivo. Evaluar el efecto de metabolitos secundarios (MS) de hongos endófitos (HE) en la inhibición micelial de Penicillium digitatum y la eficacia de control de la enfermedad, en frutos de mandarina (Citrus reticulata) después de la cosecha. Materiales y métodos. El estudio se realizó en 2022, bajo un diseño experimental completo al azar, en el laboratorio de Sanidad Vegetal, Universidad Nacional de Cañete (UNDC), Lima, Perú. Para la prueba de antibiosis se extrajeron los metabolitos secundarios de diez cepas de HE de los géneros Trichoderma sp., Fusarium sp. y Aspergillus sp., en donde se evaluó la inhibición micelial del patógeno (%). Se asperjaron MS a una concentración del 15 % (v/v) sobre frutos de mandarina que fueron desinfectados e inoculados con el patógeno por aspersión (1x105 UFC/cc). Adicionalmente, se utilizaron un biocontrol comercial, un aceite vegetal y un fungicida. Después de 6 y 9 días, se evaluaron la incidencia (%) y el índice de severidad (0,1,2,3) de la enfermedad. Resultados. Los metabolitos secundarios de las cepas HEA-111 (Aspergillus), HEA-109 (Fusarium) y HSA-1 (Trichoderma) presentaron la mayor inhibición micelial con 95,05 %, 87,51 % y 47,47 %, respectivamente. En los frutos de mandarina tratados con los MS de las cepas HEA-109 y HSA-1, hubo un control del 80 % y 55 %, respectivamente. Conclusiones. Los MS de HE tuvieron efecto en la inhibición micelial de P. digitatum y redujeron el avance de la enfermedad en frutos de mandarina después de la cosecha.

Abstract Introduction. “Green mold,” caused by Penicillium digitatum is one of the phytosanitary issues that limits citrus production. For its control, synthetic fungicides are used, which have lost effectiveness due to resistance developed by the fungus. An alternative is the use of endophytic fungi with biocontrol action. Objective. To evaluate the effect of secondary metabolites (SM) from endophytic fungi (EF) on the mycelial inhibition of Penicillium digitatum and the effectiveness of disease control in mandarin fruits (Citrus reticulata) post-harvest. Materials and methods. The study was conducted in 2022, using a completely randomized experimental design in the Plant Health Laboratory at the Universidad Nacional de Cañete (UNDC), Lima, Peru. For the antibiosis test, secondary metabolites were extracted from ten strains of EF from the genera Trichoderma sp., Fusarium sp., and Aspergillus sp., where mycelial inhibition of the pathogen (%) was evaluated. SMs were sprayed at 15 % (v/v) concentration on mandarin fruits that were disinfected and inoculated with the pathogen by spraying (1x 105 CFU/cc). Additionally, a commercial biocontrol, a vegetable oil, and a fungicide were used. After 6 and 9 days, disease incidence (%) and severity index (0, 1, 2, 3) were evaluated. Results. Secondary metabolites from strains the HEA-111 (Aspergillus), HEA-109 (Fusarium), and HSA-1 (Trichoderma) showed the highest mycelial inhibition with 95.05 %, 87.51 %, and 47.47 %, respectively. In mandarin fruits treated with SMs from strains HEA-109 and HSA-1, there was a control of 80 % and 55 %, respectively. Conclusions. SMs from EF were effective in inhibiting the mycelial growth of P. digitatum and reduces disease progression in mandarin fruits post-harvest.

Resumen El sector turístico en la actualidad está marcado por las concepciones de gestión, los espacios y la visión económica de las regiones, territorios y ciudades. Además, se aprecia que, como industria y como campo, debe contribuir al desarrollo social y comunitario sostenible en función de elevar la calidad de vida de los habitantes. El objetivo de la investigación estuvo encaminado en analizar la producción científica sobre turismo sostenible en la base de datos SCOPUS en el período 2022-2024. Para ello se implementó un diseño mixto en dos etapas, con un estudio bibliométrico como principal y una revisión hermenéutica de la literatura adaptada a los intereses cognoscitivos y metas de la investigación. Los resultados de la primera etapa evidenciaron un ligero incremento de la producción del año 2022 al año 2023, siendo las áreas más representativas las ciencias sociales y las ciencias ambientales. En la segunda etapa se examinó la relación entre sostenibilidad y turismo en el marco de los objetivos del desarrollo sostenible, así como las principales tendencias y retos.

Abstract The tourism sector today is marked by management concepts, spaces and the economic vision of regions, territories and cities. Furthermore, it is appreciated that, as an industry and as a field, it must contribute to sustainable social and community development in order to raise the quality of life of the inhabitants. The objective of the research was aimed at analyzing the scientific production on sustainable tourism in the SCOPUS database in the period 2022-2024. To achieve this, a mixed design was implemented in two stages, with a bibliometric study as the main one and a hermeneutical review of the literature adapted to the cognitive interests and goals of the research. The results of the first stage showed a slight increase in production from 2022 to 2023,0 with the most representative areas being social sciences and environmental sciences. In the second stage, the relationship between sustainability and tourism was examined within the framework of the sustainable development objectives, as well as the main trends and challenges.

Abstract Improvements in production systems that use the lean manufacturing approach known as “Lean” have the objective of eliminating or reducing waste or process inefficiencies (muda). This approach is increasingly adopted by different industrial sectors, including agriculture. Although Lean improvements are traditionally applied to existing processes from cultivation to distribution, opportunities to intervene in processing wastes (losses) are left aside. This article presents a methodological approach framed in five Lean principles, made up of six activities aimed at the design of new processes that make use of the losses generated by harvesting and agricultural transformation. The methodological approach is applied in a Colombian coffee farm where parchment coffee transformation processes are developed. A parallel losses composting process is designed that transforms defective, green, overripe beans, coffee pulp, mucilage and husk. The results show that by applying the methodological approach on the coffee farm, a level of losses transformation of 86% is achieved. Molting is also reduced for both the current parchment coffee production line and the composting line to 35% and 50% respectively. The main reductions are reprocessing and waiting times. Finally, a 30.3% reduction is obtained in the time of activities that do not add value, making both the current and proposed processes more efficient and with better delivery times.

Resumen Las mejoras en sistemas productivos que utilizan el enfoque de manufactura esbelta conocido como “Lean” tienen el objetivo de eliminar o reducir desperdicios o ineficiencias de proceso (muda). Este enfoque es adoptado por diferentes sectores industriales, entre ellos el agrícola de manera reciente. Aunque tradicionalmente se aplican mejoras Lean en procesos ya existentes desde el cultivo hasta la distribución, se dejan de lado oportunidades de intervención de pérdidas de procesamiento (mermas). Este artículo presenta un enfoque metodológico enmarcado en principios Lean, conformado por actividades orientadas al diseño de nuevos procesos que dan uso de las mermas generadas por cosecha y transformación agrícola. Se aplica el enfoque metodológico en una finca cafetera colombiana en la que se desarrollan procesos de transformación de café pergamino. Se diseña un proceso paralelo de compostaje de mermas que transforma granos defectuosos, verdes, sobremaduros, pulpa de café, mucilago y cascarilla. Los resultados demuestran que al aplicar el enfoque metodológico en la finca cafetera se logra un nivel de transformación de mermas del 86%. También se reducen las muda tanto para la línea actual de producción de café pergamino como la de compostaje al 35% y 50% respectivamente. Las principales muda reducidas son reprocesos y tiempos de espera. Finalmente se obtiene una reducción del 30,3% en el tiempo de actividades que no agregan valor haciendo de los procesos tanto actual como propuesto más eficientes y con mejores tiempos de entrega.

ABSTRACT The research work presents the conclusions of the analysis of the use of electrical energy as a resource in businesses classified as micro and small that are located in the city of San Lorenzo and registered in the same municipality. The general objective was established to “Analyze the effectiveness of electricity consumption in MSMEs in San Lorenzo and its relationship with the Green Economy in 2023”, research related to Sustainable Development Goal No. 8 Decent Work and Economic Growth that promotes inclusive and sustained economic growth as a strategy to drive progress, create decent jobs for all and improve living standards. This research had a qualitative-quantitative approach. The main results obtained were the following, 62% of the MSMEs in the city of San Lorenzo present some criterion of effectiveness in relation to the use of electrical energy, 78% of the MSMEs affirm that the quality of the supply in the electrical service It is poor and not safe. 86% of microentrepreneurs responded that electricity consumption is part of the cost of their products or services, 71% affirm that the rate for electricity consumption can be improved for the benefit of MSMEs. 65% maintain that the use of electrical energy has a direct effect on their costs and 91% of MSMEs affirm that they can use electrical energy more efficiently and rationally.

RESUMEN El trabajo de investigación presenta las conclusiones del análisis del aprovechamiento en la utilización de la energía eléctrica como recurso en los negocios tipificados como micro y pequeños que se encuentran situados en la ciudad de San Lorenzo y catastrados en el mismo municipio. Se estableció como objetivo general “Analizar la efectividad del consumo de energía eléctrica en las mipymes de San Lorenzo y su relación con la Economía verde en el año 2023”, investigación relacionada a el Objetivo de Desarrollo Sustentable N° 8 Trabajo Decente y Crecimiento Económico que promueve un crecimiento económico inclusivo y sostenido como estrategia para impulsar el progreso, crear empleos decentes para todos y mejorar los estándares de vida. Esta investigación fue de enfoque cuali-cuantitativo. Los principales resultados obtenidos fueron los siguientes, el 62% de las mipymes de la ciudad de San Lorenzo presentan algún criterio de efectividad en relación a la utilización de energía eléctrica, el 78% de las mipymes afirman que la calidad del suministro en el servicio eléctrico es deficiente y no seguro. El 86% de los microemprendedores respondieron que el consumo de energía eléctrica forma parte del costo de sus productos o servicios, el 71% afirma que la tarifa por el consumo de energía eléctrica puede ser mejorada en provecho de las mipymes. Un 65% sostiene que la utilización de la energía eléctrica tiene un efecto directo en sus costos y un 91% de las mi pymes afirman que pueden utilizar en forma más eficiente y racional la energía eléctrica.

Abstract Introduction. Corn (Zea mays) is widely used in animal feed, either as grain, green fodder or silage. Objective. To evaluate the yield and nutritional quality of 6 Costa Rican corn varieties selected for grain production. Materials and methods. The study was conducted at 4 experimental sites (Aguas Zarcas, Turrialba, San Luis de Monteverde and Guápiles) between September and December 2018. At each site, a randomized complete block design with 4 replications and 8 treatments (6 varieties and 2 corn hybrids). Plant height was evaluated, Dry Mass (DM) production, chemical composition and in vitro DM digestibility at forage harvest were evaluated. Results. Plant height and DM production were affected by treatments. Yields of the Costa Rican DM varieties EJN2, Upiav-G6, Los Diamantes 8843 and JSáenz (12.7-14 t.ha-1) were similar to the forage hybrid CLTHY002 (13.5 t.ha-1). Only DM and CP content varied in the treatments. Conclusions. The Costa Rican corn varieties EJN2, Los Diamantes 8843, JSáenz and Upiav-G6, have potential to be used as a forage source for animal feeding because they achieved yields and nutritive values like those obtained with the hybrids selected by Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) for forage production.

Resumen Introducción. El cultivo de maíz (Zea mays) es ampliamente utilizado en la alimentación animal, tanto en forma de grano, como forraje verde o ensilaje. Objetivo. Evaluar la producción y calidad nutritiva de 6 variedades de maíces costarricenses seleccionados para la producción de grano. Materiales y métodos. El estudio se realizó en 4 sitios experimentales (Aguas Zarcas, Turrialba, San Luis de Monteverde y Guápiles) entre setiembre y diciembre del 2018. En cada sitio, se trabajó con un diseño de bloques completos al azar con 4 repeticiones y 8 tratamientos (6 variedades y 2 híbridos de maíces). Se evaluó la altura de la planta, la producción de materia seca (MS), la composición química y la digestibilidad in vitro de la MS a la cosecha de los forrajes. Resultados. La altura de la planta y la producción de MS fueron afectadas por los tratamientos. Los rendimientos de MS de las variedades costarricenses EJN2, Upiav-G6, Los Diamantes 8843 y JSáenz (12,7-14 t.ha-1) fueron semejantes al híbrido forrajero CLTHY002 (13,5 t.ha-1). Solamente el contenido de MS y PC varío entre los tratamientos. Conclusión. Las variedades de maíces costarricenses EJN2, Los Diamantes 8843, JSáenz y Upiav-G6, tienen potencial para ser utilizadas como fuente forrajera para la alimentación de rumiantes, ya que alcanzaron rendimientos y valores nutritivos semejantes a los obtenidos con los híbridos seleccionados por el Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) para la producción de forrajes.

Abstract Introduction. Canavalia ensiformis, widely used as green manure, has proven benefits from mycorrhizal inoculation. The effectiveness of the arbuscular mycorrhizal inoculants used in Cuba depend on the edaphic condition in which the crop develops, however there are no experimental results on eutric chromic cambisol soils from Jibacoa. Objective. Evaluate in this soil the response of canavalia to the application of 3 mycorrhizal inoculants. Materials and methods. The research was carried out at the Jibacoa Agro-Forestry Experimental Station, Villa Clara province, Cuba. In a completely randomized design, 3 inoculants formulated with the strains INCAM-2/Funneliformis mosseae, INCAM-4/Glomus cubense and INCAM-11/Rizhophagus irregularis and a control without inoculation were studied. At the time that the canavalia flowered, the following were evaluated: the number of effective nodules; the height; the dry mass of the root, stem, leaves, total and the efficiency index was determined. Results. Canavalia presented a positive and differentiated response to mycorrhizal inoculation. The inoculation with INCAM-2 significantly increased the number of effective nodules (21.9%) with values higher than the control treatment and the inoculation with INCAM-4, it also caused the greatest height with increases of 66.1%, higher (p<0.05) to those found with the other inoculants. INCAM-2 always caused higher values of dry mass (p<0.01) in any of the organs and total in relation to the control treatment, however, they did not differ (p<0.01) from those obtained with the other inoculants, which did not always differ from the control treatment. The response based on total dry mass was ordered as follows: INCAM-2 with 71.90% > INCAM-11 with 50.17% > INCAM-4 with 38.29%. Conclusions. Canavalia presented a positive response to mycorrhizal inoculation in the eutric chromic cambisol soil with increases in its nodulation, growth and dry mass production. The INCAM-2 inoculant was always the most effective.

Resumen Introducción. Canavalia ensiformis ampliamente utilizada como abono verde, presenta beneficios comprobados por la inoculación micorrízica. La efectividad de los inoculantes micorrízicos arbusculares utilizados en Cuba dependen de la condición edáfica en que se desarrolla el cultivo; sin embargo, hay pocos resultados experimentales en suelos Cambisol éutrico crómico de Jibacoa. Objetivo. Evaluar en este suelo la respuesta de la canavalia a la aplicación de 3 inoculantes micorrizicos. Materiales y métodos. La investigación se realizó en la Estación Experimental Agro-Forestal de Jibacoa, provincia Villa Clara, Cuba. En un diseño completamente aleatorizado se estudiaron 3 inoculantes formulados con las cepas INCAM-2/Funneliformis mosseae, INCAM-4/Glomus cubense e INCAM-11/Rizhophagus irregularis y un control sin inocular. En el momento que floreció la canavalia se evaluó: la cantidad de nódulos efectivos; la altura; la masa seca de la raíz, del tallo, de las hojas, la total y se determinó el índice de eficiencia. Resultados. La canavalia presentó una respuesta positiva y diferenciada a la inoculación micorrízica. La inoculación con INCAM-2 incrementó significativamente la cantidad de nódulos efectivos (21,9%) con valores superiores al tratamiento control y a la inoculación con INCAM-4, además originó la mayor altura con aumentos del 66,1%, superiores (p<0,05) a las encontradas con los otros inoculantes. INCAM-2 siempre provocó valores superiores de masa seca (p<0,01) en cualquiera de los órganos y la total en relación con el tratamiento control, no obstante, no se diferenciaron (p<0,01) a los obtenidos con los otros inoculantes, los cuales no siempre se diferenciaron del tratamiento control. La respuesta se ordenó de acuerdo con la masa seca total como sigue: INCAM-2 con 71,90% > INCAM-11 con 50,17% > INCAM-4 con 38,29%. Conclusiones. La canavalia presentó una respuesta positiva a la inoculación micorrízica en el suelo Cambisol éutrico crómico con incrementos en su nodulación, crecimiento y producción de masa seca. El inoculante INCAM-2 siempre resultó el más efectivo.