Resumen: Las Reservas Ecológicas, son la base primordial para la subsistencia de la biodiversidad, desempeñan funciones cruciales para el mantenimiento de los ecosistemas, especialmente, en la preservación de los servicios ecosistémicos de regulación del clima y suministro de agua. Determinar la capacidad de acumulación del COS en tres ecosistemas de la Reserva Ecológica Arenillas (REAR) y la influencia que tienen con los factores de profundidad y el tipo de suelo, fue el objetivo de la investigación. Se partió de la hipótesis que dichos factores ejercen un efecto sobre las propiedades físicas-químicas del suelo, provocando la variabilidad del carbono. Se establecieron 14 unidades de muestreo para los tres ecosistemas; 1) Intervenido, 2) Bosque deciduo y 3) Bosque arbustal deciduo, se extrajo de cada unidad dos muestras de suelo a profundidades de (0-10 y 10-30 cm), dando un total de 28 muestras. Los resultados revelaron diferencias significativas entre los tres tipos de ecosistemas, permitiendo afirmar que, en la REAR, el contenido de COS es mayor en el ecosistema de Bosque deciduo, que en los denominados Bosque arbustal deciduo y la zona Intervenida. Así mismo, los suelos del tipo Alfisol y Entisol en el Bosque deciduo presentaron la mayor capacidad de almacenamiento de COS, siendo la capa superficial la de mayor acumulación en los tres ecosistemas. Se concluyó que las bajas precipitaciones, el aumento de la temperatura y la esporádica vegetación producida por actividades antrópicas, afectan el almacenamiento de COS, limitando la prestación de servicios ecosistémicos relacionados con la captura de carbono edáfico.

Abstract: Ecological Reserves are the primary basis for the subsistence of biodiversity, they play crucial roles in the maintenance of ecosystems, especially in the preservation of ecosystem services of climate regulation and water supply. The objective of the research was to determine the accumulation capacity of COS in three ecosystems of the Arenillas Ecological Reserve (REAR) and the influence of depth factors and soil type. It was hypothesized that these factors exert an effect on the physical-chemical properties of the soil, causing carbon variability. Fourteen sampling units were established for the three ecosystems; 1) Intervened, 2) Deciduous Forest and 3) Deciduous shrub forest, two soil samples were extracted from each unit at depths of (0-10 and 10-30 cm), giving a total of 28 samples. The results revealed significant differences between the three types of ecosystems, allowing us to affirm that, in the REAR, the COS content is higher in the deciduous forest ecosystem than in the deciduous shrub forest and the Intermediate zone. Likewise, Alfisol and Entisol soils in the deciduous forest had the highest COS storage capacity, being the surface layer the one with the highest accumulation in the three ecosystems. It was concluded that low precipitation, increased temperature and sporadic vegetation produced by anthropic activities affect COS storage, limiting the provision of ecosystem services related to soil carbon sequestration.

Abstract: Background: Through field work in the municipality of Altotonga, Veracruz, populations of a species of the genus Matelea with a small flower were located. This entity did not match with the species reported for Mexico, but it is morphologically similar to M. anomala, which is endemic to Bolivia. Questions: Do the Altotonga Matelea populations correspond to a new species for science or it is a disjunct distribution of M. anomala? Studied species: Matelea sp. nov. Study site and dates: Pancho Poza River Reserve, Altotonga, Veracruz, Mexico. Methods: A morphological comparison was made with Mexican species of Matelea and with M. anomala, using the cohesive species concept. Results: Matelea lilliputiana is described as a new species to science, endemic to the municipality of Altotonga, Veracruz. It differs from M. anomala in having a 5-lobed gynostegial crown with bifurcate apices (vs. crown not clearly lobed and fimbriate margins in M. anomala), angular stipe (vs. cylindrical) and a densely pubescent-hirsute calyx (vs. glabrescent). Additionally, M. lilliputiana is endemic to Mexico and grows in pine-oak forest with mesophyll elements, while M. anomala is endemic to Bolivia and grows in seasonally dry scrub. Conclusions: With the present discovery, the state of Veracruz reaches 126 species of Apocynaceae, with five endemics. Worldwide, it is the second species of the genus with corollas less than 5 mm long and the first in Mexico.

Resumen: Antecedentes: En el trabajo de campo realizado en el municipio de Altotonga, Veracruz, se localizaron poblaciones de una especie del género Matelea con flor sobresalientemente pequeña, que no coincidía con las especies reportadas para México, sino que es morfológicamente similar a M. anomala, especie endémica de Bolivia. Preguntas: ¿las poblaciones de la Matelea de Altotonga corresponden a una especie nueva para la ciencia o a una distribución disjunta de M. anomala? Especies de estudio: Matelea sp. nov. Sitio y años de estudio: Reserva del Río Pancho Poza, Altotonga, Veracruz, México. Métodos: Se realizó comparación morfológica con las especies mexicanas de Matelea y con M. anomala, empleando el concepto cohesivo de especie. Resultados: Se describe a Matelea lilliputiana como especie nueva para la ciencia, endémica del municipio de Altotonga, Veracruz. Se diferencia de M. anomala por presentar una corona ginostegial 5-lobada con ápices bifurcados (vs. corona no claramente lobada y márgenes fimbriados en M. anomala), estípite anguloso (vs. cilíndrico) y cáliz densamente pubescente-hirsuto (vs. glabrescente). Adicionalmente, M. lilliputiana es endémica de México y crece en bosque de pino-encino con elementos de mesófilo, mientras que M. anomala es endémica de Bolivia y crece en matorral estacionalmente seco. Conclusiones: Con el presente descubrimiento, el estado de Veracruz alcanza 126 especies de Apocynaceae, con cinco endémicas. A nivel mundial, es la segunda especie del género en presentar corolas menores a 5 mm de largo y la primera para México.

Abstract (Introduction): In Latin America, the sensitive tropical biodiversity found outside of protected wilderness areas is under threat. In Costa Rica, the payment for environmental services (PSA) represents a mechanism to protect forests on private farms, but it lacks specific indicators on the state of biodiversity and forest conservation. (Objective): To analyze the use of acoustic monitoring for ecosystem assessment at 11 sites in the Osa Peninsula, nine on private farms, and two sites within Corcovado National Park. (Methods): Soundscapes were recorded from May 25 to June 1, 2021, using acoustic recorders. It was analyzed the behavior of six acoustic indices at each sampling site and applied an automated detection analysis of vocalizations of two representative bird species of the area. (Results): It was found that the most fragmented sites presented higher activity, energy, and acoustic diversity, possibly due to edge effects and ecotones present, as well as a high forest component in the surrounding matrix. Automated vocalization detection indicated that the species Hylopezus perspicillatus turned out to be more sensitive to differences in the landscape than Trogon bairdii. (Conclusions): The use of acoustic indices, although it associates features of the degree of conservation, was not a differentiating element of the quality of the ecosystem in the evaluated farms. The monitoring of species through the detection of their vocalizations was promising. A careful and rigorous sampling design, with more sampling points per site, is recommended to achieve accurate measurements and avoid bias in the analysis.

Resumen (Introducción): En Latinoamérica, la sensible biodiversidad tropical que se encuentra fuera de las áreas silvestres protegidas está bajo amenaza. En Costa Rica, el pago por servicios ambientales (PSA) representa un mecanismo para proteger bosques en fincas privadas, pero carece de indicadores específicos sobre el estado de la biodiversidad y de conservación de los bosques. (Objetivo): Analizar el uso del monitoreo acústico para la evaluación de ecosistemas en 11 sitios en la Península de Osa: 9 en fincas privadas y 2 sitios dentro del Parque Nacional Corcovado. (Metodología): Se grabaron paisajes sonoros del 25 de mayo al 01 de junio del 2021, utilizando grabadoras acústicas. Se estudia el comportamiento de 6 índices acústicos en cada sitio de muestreo y se aplicó un análisis de detección automatizada de vocalizaciones de 2 especies de aves representativas de la zona. (Resultados): Se encuentra que los sitios más fragmentados presentaron mayor actividad, energía y diversidad acústica, posiblemente por efectos de borde y ecotonos presentes, así como un alto componente de bosque en la matriz circundante. La detección automatizada de vocalizaciones indicó que la especie Hylopezus perspicillatus resultó ser más sensible a las diferencias en el paisaje que Trogon bairdii. (Conclusiones): El uso de índices acústicos, aunque asocia rasgos del grado de conservación del bosque, no resultó un elemento diferenciador de la calidad del ecosistema en las fincas evaluadas, pero el monitoreo de especies mediante la detección de sus vocalizaciones sí resultó promisorio. Es recomendable un diseño de muestreo metódico y riguroso, con más puntos de muestreo por sitio, para lograr mediciones precisas y evitar sesgos en el análisis.

Schinopsis brasiliensis Engl. (braúna) is a tree species of the Anacardiaceae family. It is native to Brazil, Paraguay, and Bolivia. It is a candidate for agro-industrial system that can be used to produce phytotherapeutics and bioproducts. However, to comprehensively understand the characteristics of plant within the framework of sustainable development for agro-industries, exploring the chemical and phenotypic diversity of samples from various microenvironments across different collection periods is essential. Accordingly, herein, the influence of different environmental factors and sample collection durations on braúna samples was investigated over several years using multivariate statistical analyses associated with nuclear magnetic resonance (NMR) spectroscopy. Bark samples from different areas and environmental data from different locations were analyzed. Distinct chemical profiles were observed across reserve, forest, and management areas, all marked by elevated sugar levels suggesting water stress adaptation. High radiation and low rainfall correlated with increased sugar and amino acids. Each microenvironment responded uniquely to these environmental conditions. Consequently, plant samples from different microenvironments collected over different periods were observed to respond differently to environmental factors, such as solar radiation and temperature. We correlated the metabolomic data with climatic data evaluating the influence of abiotic factors on the production of metabolites in braúna bark samples. Engl (braúna family Brazil Paraguay Bolivia agroindustrial agro industrial bioproducts However agroindustries, agroindustries industries, industries agro-industries essential Accordingly herein NMR (NMR spectroscopy analyzed reserve forest adaptation acids conditions Consequently temperature

Abstract The lowland Atlantic Forest, at altitudes of below 500 m, is highly fragmented, and is home to many threatened species of birds. The Brazilian state of Rio de Janeiro has a high concentration of threatened birds in the Americas and is an important residual of the dense ombrophilous formations of the Atlantic Forest, which includes the Reserva Biológica União, a high priority area for conservation of Atlantic Forest birds. This study compiled empirical and secondary records of threatened birds in this reserve and discusses potential factors determining their local occurrence. Since 2008, regular observations, including transect surveys and mist-netting, in addiction to secondary data, have provided records of 306 bird species, of which 49 appear on red lists at some level (state, national or global), including 34 listed as endangered, of which 13 are listed globally, eight of them dependent on well-conserved lowland rainforest. Future studies should include new areas of the reserve, with the aim of confirming the presence of additional threatened species. The long-term conservation of this fauna will depend not only on the adequate management of the reserve, but also the reestablishment of its connectivity with adjacent forest fragments and upland areas in the state. 50 m fragmented União occurrence 2008 observations mistnetting, mistnetting mist netting, netting mist-netting data 30 4 state, (state global, global , global) 3 endangered 1 globally wellconserved well conserved rainforest longterm long term 5 200 20 2

Abstract The objective of this work was to evaluate the potential of native species used in ecological restoration in Brazil (Peltophorum dubium - canafístula, Hymenaea courbaril - jatobá, Jacaranda cuspidifolia - jacarandá, and Enterolobium contortisiliquum - timboril) as raw material for the supply of sawmills. Due to the difficulties in sampling trees of native species, four trees per species were collected, at age of 12 years, in a legal reserve area. The sampled trees were evaluated as being quite characteristic of trees of the same genetic material. The trees’ diameter was measured, the percentage of bark and the taper were measured, and the lumber yield of each species was calculated. The boards were air-dried. Based on the DBH, timboril had the greatest potential for biomass production, but its wood quality was unsatisfactory. To supply raw material for sawmills, in addition to jatobá, we suggest investing in plantations of canafístula and jacarandá. Peltophorum jatobá jacarandá sawmills collected 1 years area measured calculated airdried. airdried air dried. dried air-dried DBH production unsatisfactory

Abstract Threats must be eliminated from the tropical mountain forest habitat of Dolok Sipirok Natural Reserve, which is home to the Tapanuli Orangutan (Pongo tapanuliensis). The threat arises from human-orangutan conflict resulting from the occupation of agricultural land and the opening of accessibility. The aim of this research is to identify areas and patterns of conflict between orangutans and humans in the vicinity of the Dolok Sipirok Nature Reserve. Data was gathered through community interviews and direct observation of the Tapanuli orangutan. The magnitude of community losses was determined by analyzing economic losses and crop destruction. The majority of conflicts were discovered in community fields that were about to be harvested. The main attraction for orangutans is fruit plants in those areas. These fruit trees are located in a roaming area and animal corridor. A buffer zone must be managed effectively to provide for the orangutan’s sustenance and nesting requirements. Reserve Pongo tapanuliensis. tapanuliensis . tapanuliensis) humanorangutan human orangutan accessibility destruction harvested corridor s requirements

RESUMO Trilhas ecológicas são propostas para auxiliar na Educação Ambiental, sobretudo em Unidades de Conservação, promovendo uma educação dinâmica e interação entre os visitantes e a natureza. A Floresta Nacional de Açu é uma Unidade de Conservação na Caatinga, localizada no município de Açu, Rio Grande do Norte. Aqui objetivamos desenvolver uma trilha de Educação Ambiental para visitantes, coletores de sementes, parataxonomistas e pesquisadores. Os indivíduos arbóreos foram marcados com placas de alumínio numeradas, identificadas e georreferenciadas. Foram identificadas 13 espécies de Leguminosae e marcados 34 indivíduos, além de 58 indivíduos pertencentes a 13 famílias distintas. Foram definidas três trilhas: (1) Trilha das Leguminosas; (2) Trilha da Catingueira-cinzenta e (3) trilha de imersão na Caatinga. Essas trilhas foram testadas pelos autores e visitantes nos dias 23-24 julho de 2022. Concluímos que as trilhas se tornam uma importante ferramenta para proporcionar a proteção destes ambientes e uma maior percepção das espécies locais. natureza Caatinga Norte sementes pesquisadores numeradas georreferenciadas 1 3 5 distintas (1 Leguminosas 2 (2 Catingueiracinzenta Catingueira cinzenta (3 2324 23 24 23-2 2022 locais ( 232 23- 202 20

ABSTRACT Ecological trails are proposed to assist in environmental education programs, especially in reserves promoting a dynamic education and interaction with nature. The Floresta Nacional de Açu is a reserve of Caatinga dry forest, located in Açu, Rio Grande do Norte, Brazil. Here we aimed to develop an environmental education trail for visitors, seed collectors, parataxonomists and researchers. Adult tree individuals were marked with numbered aluminum tags, identified and georeferenced. Thirteen species of the Leguminosae were identified and 34 individuals were tagged, in addition to 58 individuals belonging to 13 different families. Three trails were defined: (1) Trail of Legumes; (2) Catingueira-cinzenta Trail; and (3) immersion trail in the Caatinga. These trails were tested by the authors and visitors between july 23-24, 2022. We conclude that the trails become an important tool to provide the protection of these environments and to increase perception of the local species. programs nature forest Norte Brazil collectors researchers tags georeferenced 3 tagged 5 1 families defined (1 Legumes 2 (2 Catingueiracinzenta Catingueira cinzenta (3 2324, 2324 23 24, 24 23-24 2022 ( 232 23-2 202 23- 20

ABSTRACT We sought to evaluate the floristic and structural variations between three areas with distinct backgrounds in the Sooretama Biological Reserve, remnantd of Lowland Atlantic Forest in Brazil. For vegetation sampling, 30 transects were established in three different areas: post-pasture (PAS), post-fire (FIR), and preserved (PRE) remnants, totaling a sample area of 0.3ha. All individuals in the woody layer with diameter at breast height ≥ 2.5cm were sampled. Variations in floristic composition were evaluated by means of NMDS, based on the presence or absence of species. There are clear variations between the three areas evaluated, in which the PRE area presents greater species richness, floristic diversity, and basal area, with parameters similar to forests of high diversity in northern Espírito Santo and southern Bahia. The performance of inventories and monitoring of the remnants of Lowland Atlantic Forests in neglected regions are fundamental for planning conservation and enrichment measures of these vegetations Reserve Brazil sampling 3 postpasture post pasture PAS, PAS , (PAS) postfire fire FIR, FIR (FIR) (PRE 03ha ha 0 3ha 0.3ha 25cm cm 2 5cm sampled NMDS richness Bahia (PAS (FIR

RESUMO Buscamos avaliar as variações florísticas e estruturais entre três áreas com origens distintas na Reserva Biológica de Sooretama. Para amostragem da vegetação, foram estabelecidos 30 transectos em três áreas distintas: pós-pasto (PAS), pós-fogo (FIR) e remanescentes conservados (PRE), totalizando uma área amostral de 0,3ha. Todos os indivíduos da camada lenhosa com diâmetro à altura do peito ≥ 2,5 cm foram amostrados. As variações na composição florística foram avaliadas por meio do NMDS, com base na presença ou ausência de espécies. Há claras variações entre as três áreas avaliadas, sendo que a área do PRE apresenta maior riqueza de espécies, diversidade florística e área basal, com parâmetros semelhantes às florestas de alta diversidade do norte do Espírito Santo e sul da Bahia. A realização de inventários e monitoramento dos remanescentes de Floresta Ombrófila Densa de Terras Baixas em regiões negligenciadas são fundamentais para o planejamento de medidas de conservação e enriquecimento dessas vegetações. Sooretama vegetação 3 póspasto pós pasto PAS, PAS , (PAS) pósfogo fogo FIR (FIR PRE, (PRE) 03ha ha 0 3ha 0,3ha 25 2 5 2, amostrados NMDS espécies basal Bahia vegetações (PAS (PRE

Abstract A new species of Cambeva occurring in the rio Piquiri and Ivaí, upper rio Paraná basin, Brazil, is described using the combination of morphological and molecular data. The new species is distinguished from most congeners by the presence of a notch in the posterior portion of the metapterygoid, number of branchiostegal rays, opercular and interopercular odontodes, and ribs. In addition, the results corroborated the existence of a single species with wide intraspecific variation in body coloration. The type-locality is within the area of influence of the Perobas Biological Reserve, a Conservation Unit in the Paraná State, composed of two Atlantic Forest physiognomic forms. Considering that the upper rio Paraná basin is an area of significant anthropic influence, it is crucial to describe and preserve species to understand their ichthyofauna. Ivaí Brazil data metapterygoid rays odontodes ribs addition coloration typelocality type locality Reserve State forms ichthyofauna

Resumo Uma espécie nova de Cambeva com ocorrência em tributários dos rios Piquiri e Ivaí, bacia do alto rio Paraná, Brasil, é descrita utilizando a combinação de dados morfológicos e moleculares. A espécie nova pode ser diferenciada de suas congêneres pela presença de um entalhe na porção posterior do metapterigóide, número de raios branquiostegais, odontódeos operculares e interoperculares, e de costelas. Além disso, os resultados corroboraram a existência de uma única espécie com ampla variação intraespecífica no padrão de coloração corporal. A localidade-tipo está situada na área de influência da Reserva Biológica das Perobas, uma Unidade de Conservação no estado do Paraná, composta por duas fisionomias vegetais da Mata Atlântica. Considerando que a bacia do alto rio Paraná é uma área de influência antrópica, é fundamental descrever e preservar suas espécies para conhecer sua ictiofauna. Ivaí Brasil moleculares metapterigóide branquiostegais interoperculares costelas disso corporal localidadetipo localidade tipo Perobas Atlântica antrópica ictiofauna

Resumen (Introducción): La restauración ecológica es una prioridad global para recuperar la biodiversidad y alcanzar los Objetivos de Desarrollo Sostenible. Desde su creación en el año 2000, la Reserva Natural Madre Verde en Costa Rica ha sido un modelo en este sentido. En 2005 se realizó una caracterización de las comunidades vegetales presentes: pastizal, charral, cultivo abandonado, área dominada por burío, bosque secundario denso, bosque secundario ralo y dos remanentes de bosque maduro. (Objetivo): En 2020 se repitió el estudio, excluyendo los remanentes de bosque maduro, con el fin de evaluar los cambios en la vegetación después de 15 años de restauración. (Metodología): Se identificaron las especies de árboles, se calcularon índices de riqueza y diversidad y se elaboraron perfiles de vegetación en cada tipo de comunidad. (Resultados): Los mayores cambios en los índices de riqueza y diversidad, composición y estructura vertical de la vegetación se observaron en las zonas de pastizal que fueron sometidas a reforestación activa. Además, se registraron cambios importantes en el charral y en la zona dominada por burío. Los menores cambios se dieron donde ya existía algún tipo de bosque secundario relativamente maduro debido a la sombra creada por los árboles. (Conclusiones): En todos los sitios predominan las especies pioneras nativas de esta zona de vida, lo que garantiza el proceso natural de regeneración. Estos resultados permiten conocer los avances en la restauración y saber cuáles son las especies más adecuadas para reforestar otras áreas similares y asegurar la recuperación del bosque secundario de manera más rápida y efectiva.

Abstract (Introduction): Ecological restoration is a global priority for recovering biodiversity and achieving Sustainable Development Goals. Since its creation in 2000, the Madre Verde Natural Reserve in Costa Rica has been a model in this regard. In 2005, a characterization of the present vegetation communities was conducted, including grassland, shrubland, abandoned crop, area dominated by burío, dense secondary forest, sparse secondary forest, and two remnants of mature forest. (Objective): In 2020, the study was repeated, excluding the remnants of mature forest, to evaluate changes in vegetation after 15 years of restoration. (Methodology): Tree species were identified, richness and diversity indices were calculated, and vegetation profiles were developed for each community type. (Results): The greatest changes in vegetation richness and diversity indices, composition, and vertical structure were observed in grassland areas that underwent active reforestation. Additionally, significant changes were recorded in shrubland, and the area dominated by burío. The least changes were observed where some type of relatively mature secondary forest already existed due to the shade created by the trees. (Conclusions): Pioneering native species from this life zone predominate in all sites, ensuring the natural regeneration process. These results allow us to know the advances in restoration and which species are most suitable for reforesting other similar areas and ensuring the recovery of secondary forest more quickly and effectively.

ABSTRACT Sixty-two species of fungi from Argentina have been listed in different categories of the International Union for Conservation of Species (IUCN). Rickiella edulis (Speg.) Pfister is known as an ascoma-forming species of considerable size, easy to identify and recognize in the field. The number of records of this species in Argentina is still scarce. The two natural ecosystems in which it has been record- ed are in alarming decline due to their conversion to agricultural and livestock activities and urban growth. Those factors led the IUCN to categorize R. edulis as an Endangered species in 2020. Additionally, other sixty-two species of fungi from Argentina are currently included in different IUCN categories. This paper provides the first review of the Argentine species included in the IUCN Red List of Species reviewed in the unit “The Global Fungal Red List Initiative”, with emphasis on relevant species. This work also provides new information on the geographic distribution of R. edulis in Argentina, which has had no new contributions since 2016, incorporating the first record for the Yungas Biosphere Reserve in the province of Jujuy, NW Argentina.

RESUMEN Sesenta y dos especies de hongos de Argentina han sido incluidas en diferentes categorías de la Unión Internacional para la Conservación de las Especies (UICN). Rickiella edulis (Speg.) Pfister es conocida como una especie formadora de ascomas de tamaño considerable, de fácil identificación y reconocimiento a campo y aun así el número de registros de esta especie es bajo en Argentina. Los dos ecosistemas na- turales en los que ha sido registrada se encuentran en alarmante estado de retroceso por su reconversión para actividades agrícola-ganaderas y por el crecimiento urbano; por lo cual la especie ha sido categorizada como Amenazada (Endangered) por la UICN en 2020. El presente trabajo proporciona la primera revisión de las especies Argentina incluidas en la Lista Roja de Especies de la UICN revisadas en la unidad “The Global Fungal Red List Initiative”, con énfasis en las especies relevantes; y contribuye con nueva información respecto a la distribución geográfica de R. edulis en la Argentina, que no contaba con nuevos aportes desde el año 2016, incorporando el primer registro para la Reserva de la Biosfera de las Yungas en la provincia de Jujuy, NO de Argentina.

Resumen Introducción: La falta de conocimiento sobre la germinación de semillas y el establecimiento de plántulas es una de las principales limitaciones para la restauración de áreas degradadas, incluido el bosque seco tropical conocido como Caatinga. Objetivo: Evaluar la movilización de reservas y metabolitos secundarios durante estas etapas de desarrollo en Erythina velutina. Métodos: Las semillas fueron escarificadas, desinfectadas, embebidas, sembradas entre toallas de papel e incubadas bajo condiciones controladas. Cultivamos las plántulas hidropónicamente en un invernadero. Recolectamos los cotiledones en la imbibición de la semilla, la protrusión de la radícula, la emergencia del hipocótilo, la formación del gancho apical y la expansión de las hojas cordiformes, la primera y segunda hoja trifoliada. Resultados: Las semillas contenían 20 % de almidón, 14.5 % de proteínas de almacenamiento, 11.6 % de lípidos neutros y 5.7 % de azúcares no reductores en peso seco. Los azúcares solubles se consumieron desde la emergencia del hipocótilo hasta la formación del gancho apical. Las principales reservas se movilizaron desde la formación del gancho apical hasta la expansión de la primera hoja trifoliada. La actividad enzimática aumentó desde la mitad hasta el final del establecimiento de las plántulas, movilizando almidón, aceites y proteínas. Se detectaron derivados de terpenoides, flavonoides, ácidos fenólicos y alcaloides. Los flavonoides y los ácidos fenólicos estuvieron en casi todas las etapas y los derivados terpenoides desaparecieron en la expansión de las hojas cordiformes. Conclusión: Los azúcares solubles apoyan el crecimiento temprano de las plántulas; el almidón, los aceites y las proteínas se movilizan simultáneamente desde el establecimiento medio hasta el final por amilasas, lipasas y proteasas ácidas. Los cotiledones contienen metabolitos secundarios, que pueden actuar en la defensa de las plántulas. El alto contenido de reservas y los metabolitos secundarios en los cotiledones podría permitir que las plántulas de E. velutina toleren estrés, validando su uso en la restauración de áreas degradadas.

Abstract Introduction: The lack of knowledge on seed germination and seedling establishment is a main constraint for the restoration of degraded areas, including the tropical dry forest known as Caatinga. Objective: To assess reserve and secondary metabolite mobilization during seed germination and seedling establishment in Erythina velutina. Methods: We scarified, disinfected, imbibed, sown between towel paper, and incubated seeds under controlled conditions. We hydroponically cultivated seedlings in a greenhouse. We harvested cotyledons at seed imbibition, radicle protrusion, hypocotyl emergence, apical hook formation and expansion of cordiform leaves, first trifoliate leaf, and second trifoliate leaf. Results: Seeds contained approximately 20 % starch, 14.5 % storage proteins, 11.6 % neutral lipids, and 5.7 % non-reducing sugars on a dry weight basis. Soluble sugars were mainly consumed from hypocotyl emergence to apical hook formation, while major reserves were mobilized from apical hook formation to expansion of first trifoliate leaf. Enzymatic activity increased from mid to late seedling establishment, causing the mobilization of starch, oils, and proteins. Terpenoid-derivatives, flavonoids, phenolic acids, and alkaloids were detected. Flavonoids and phenolic acids were present at almost all stages and terpenoid-derivatives disappeared at expansion of cordiform leaves. Conclusion: Soluble sugars support early seedling growth, while starch, oils and proteins are simultaneously mobilized from mid to late establishment by amylases, lipases, and acid proteases. The cotyledons contain secondary metabolites, which may act in seedling defense. High content of reserves and presence of secondary metabolites in the cotyledons could enable E. velutina seedlings endure stress, validating their use in the restoration of degraded areas.

ABSTRACT Afforestation and reforestation were the challenges the farmer can seize to plant a culture that can capture more carbon than the amount emitted for cultivation. Assuming that the land was legally rented and owned, and part of an area that had not been obtained through recent deforestation, the main questions were: “why a farmer should have preferred to reserve the area to plant trees?”; and “How much did one ton of Carbon Dioxide [CO2] have to be rewarded to buy this opportunity?” This work had the target to estimate which was the minimum price for carbon credit so that the farmer will plant a forest instead of using the soil for grain cultivation. Based on the analysis that economic aspects and profit were the main drivers considered by the farmer to decide how to use the soil in case the area was not classified as Legal Reserve or Permanent Protection Area, seeking the usage which maximized the value per hectare. Considering a eucalyptus commercial forest planted under the premises of the current study, results showed that a price of around 24 BRL per ton of CO2 in 2021 is enough to turn it economically feasible. Business case had been estimated with and without profit coming from the commercial use of forest, and even assuming that no wood is cut and sold, the 2021 price of 40,48 BRL per ton of CO2 can ensure more profit than grain production over 14 years timeframe, allowing the farmer to make money beyond the usual commercial use of a forest. cultivation owned deforestation why trees trees?” How CO [CO2 opportunity opportunity? Area hectare study 2 202 feasible sold 4048 40 48 40,4 1 timeframe trees? [CO 20 404 4 40,

RESUMO Florestar e reflorestar foram os desafios que o agricultor pode enfrentar para plantar uma cultura capaz de capturar mais carbono do que a quantidade emitida para o cultivo. Partindo do pressuposto de que a terra era legalmente arrendada e própria, e parte de uma área que não havia sido obtida por meio de desmatamento recente, as principais questões eram: “por que um agricultor teria preferido reservar a área para plantar árvores?” e “Quanto uma tonelada de Dióxido de Carbono [CO2] teve que ser recompensada para comprar esta oportunidade?” Este trabalho teve como objetivo estimar qual o preço mínimo do crédito de carbono para que o agricultor plante uma floresta ao invés de usar o solo para cultivo de grãos. Com base na análise, os aspectos econômicos e de lucro foram os principais direcionadores considerados pelo agricultor para decidir como usar o solo caso a área não fosse classificada como Reserva Legal ou Área de Proteção Permanente, buscando o uso que maximizasse o valor por hectare. Considerando uma floresta comercial de eucalipto plantada nas dependências do presente estudo, os resultados mostraram que um preço em torno de R$ 24 por tonelada de CO2 em 2021 é suficiente para torná-la economicamente viável. O caso de negócios foi estimado com e sem lucro proveniente do uso comercial da floresta e, mesmo assumindo que nenhuma madeira seja cortada e vendida, o preço de 2021 de R$ 40,48 por tonelada de CO2 pode garantir mais lucro do que a produção de grãos ao longo de 14 anos, permitindo ao agricultor ganhar dinheiro além do uso comercial usual de uma floresta. própria recente eram árvores árvores? Quanto CO [CO2 oportunidade oportunidade? análise Permanente hectare estudo R 2 202 tornála torná la viável vendida 4048 40 48 40,4 1 anos [CO 20 404 4 40,

Resumen Antecedentes: En la región de la Reserva de la Biosfera Sierra del Abra Tanchipa, la apicultura es una alternativa de producción sostenible. Su manejo, requiere conocer la riqueza, distribución y fenología de la flora melífera. Preguntas: ¿Cuáles son las especies melíferas del paisaje aledaño a la Reserva? ¿Cuál es su origen, forma de crecimiento, fenología y distribución? ¿Cómo es la riqueza en áreas naturales y perturbadas? Sitio y periodo de estudio: Área de influencia a la Reserva de la Biósfera Sierra del Abra Tanchipa, San Luis Potosí, México, agosto 2020 - marzo 2022. Métodos: Se recolectaron especies melíferas mensualmente en radios de pecoreo de seis apiarios, en diferentes tipos de vegetación. Se registró su forma de crecimiento, origen, recurso floral y fenología. Se comparó riqueza y composición de especies entre tipos de vegetación de áreas naturales y perturbadas. Resultados: Se registraron 191 especies melíferas; 91 nectaríferas, 16 poliníferas y 84 néctar-poliníferas. Fabaceae es la familia más representativa. La floración abunda en junio y septiembre, disminuye en enero y abril. El 49 % de especies habitan exclusivamente áreas perturbadas, 29 % áreas naturales, 22 % ambas. En áreas naturales 100 % son nativas, en áreas perturbadas 86 % son nativas y 14 % introducidas; en áreas naturales 70 % son árboles y en áreas perturbadas 57 % son hierbas. Conclusiones: La región presenta una gran riqueza de flora melífera nativa. La información generada permitirá a los apicultores mejorar el manejo de sus colmenas y valorar las áreas naturales de pecoreo.

Abstract Background: Beekeeping is an alternative for sustainable production in Sierra del Abra Tanchipa Biosphere Reserve region. Knowing richness of melliferous flora, its distribution and flowering season, improves its management. Questions: Which are the species of melliferous flora in the surrounding landscape of the Reserve? Which is its origin, growth form, floral resource, phenology and distribution? How is species richness in natural areas and disturbed areas? Study site and period: Influence area of Sierra del Abra Tanchipa Biosphere Reserve, San Luis Potosí, Mexico, between August 2020 to March 2022. Methods: Melliferous flora species were collected monthly in bee foraging radius of six apiaries, in different types of vegetation. Data was taken from growth form, origin, floral resource offered, and phenology. Species richness and composition were compared between vegetation types from natural and disturbed areas. Results: 191 melliferous species were recorded; 91 nectariferous, 16 polliniferous and 84 nectar-polliniferous. Fabaceae is the most representative family. Flowering increases in June and September, decreases in January and April. Forty nine percent of species exclusively inhabit disturbed areas, 29 % natural areas and 22 % both. In natural areas 100 % are native while in disturbed areas 14 % are introduced and 86 % native. In natural areas 70 % are trees while in disturbed areas 57 % are herbs. Conclusions: The region has a great richness of native melliferous flora. The information generated will allow beekeepers to have a better management of their hives and to value the natural areas of foraging.