Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by extracellular accumulation of amyloid-β (Aβ) peptides in the brain. This study demonstrates the direct association of the disposable pipette extraction (DPX) with tandem mass spectrometry (MS/MS) for the analysis of Aβ peptides in cerebrospinal fluid (CSF) samples. Different parameters were optimized in order to improve detectability in the MS/MS including mobile phase percentage of ammonium hydroxide, mobile phase flow rate and acquisition mode. Also, this method used an electrospray ionization (ESI) low-flow probe and direct infusion of an organic solution in the MS/MS. The DPX-MS/MS method showed adequate linearity for determining Aβ peptides in CSF-linearity ranged from 0.1 to 1.5 ng mL 1. The coefficients of determination were higher than 0.99; the precision coefficient of variation (CV) ranged from 0.3 to 12.7%; and the accuracy relative standard deviation (RSD) ranged from -13.6 to 13.2%. Alzheimers Alzheimer s AD (AD amyloidβ amyloid β (Aβ brain DPX (DPX MSMS MS (MS/MS CSF (CSF samples hydroxide mode Also ESI (ESI lowflow low DPXMS/MS DPXMSMS CSFlinearity 01 0 1 0. 15 5 0.99 099 99 CV (CV 03 3 12.7% 127 12 7 RSD (RSD 13.6 136 13 6 -13. 132 2 13.2% DPXMS 0.9 09 9 12.7 13. -13 13.2 12. -1 -

ABSTRACT Purpose: The epithelial–mesenchymal transition of human lens epithelial cells plays a role in posterior capsule opacification, a fibrotic process that leads to a common type of cataract. Hyaluronic acid has been implicated in this fibrosis. Studies have investigated the role of transforming growth factor (TGF)-β2 in epithelial–mesenchymal transition. However, the role of TGF-β2 in hyaluronic acid-mediated fibrosis of lens epithelial cell remains unknown. We here examined the role of TGF-β2 in the hyaluronic acid-mediated epithelial–mesenchymal transition of lens epithelial cells. Methods: Cultured human lens epithelial cells (HLEB3) were infected with CD44-siRNA by using the Lipofectamine 3000 transfection reagent. The CCK-8 kit was used to measure cell viability, and the scratch assay was used to determine cell migration. Cell oxidative stress was analyzed in a dichloro-dihydro-fluorescein diacetate assay and by using a flow cytometer. The TGF-β2 level in HLEB3 cells was examined through immunohistochemical staining. The TGF-β2 protein level was determined through western blotting. mRNA expression levels were determined through quantitative real-time polymerase chain reaction. Results: Treatment with hyaluronic acid (1.0 μM, 24 h) increased the epithelial–mesenchymal transition of HLEB3 cells. The increase in TGF-β2 levels corresponded to an increase in CD44 levels in the culture medium. However, blocking the CD44 function significantly reduced the TGF-β2-mediated epithelial–mesenchymal transition response of HLEB3 cells. Conclusions: Our study showed that both CD44 and TGF-β2 are critical contributors to the hyaluronic acid-mediated epithelial-mesenchymal transition of lens epithelial cells, and that TGF-β2 in epithelial-mesenchymal transition is regulated by CD44. These results suggest that CD44 could be used as a target for preventing hyaluronic acid-induced posterior capsule opacification. Our findings suggest that CD44/TGF-β2 is crucial for the hyaluronic acid-induced epithelial-mesenchymal transition of lens epithelial cells. Purpose epithelialmesenchymal mesenchymal opacification cataract TGFβ2 TGFβ TGF β2 β (TGF)-β However TGF-β acidmediated mediated unknown Methods HLEB (HLEB3 CD44siRNA CDsiRNA siRNA CD 300 reagent CCK8 CCK 8 CCK- viability migration dichlorodihydrofluorescein dichloro dihydro fluorescein cytometer staining blotting realtime real time reaction Results 1.0 10 1 0 (1. μM 2 h CD4 medium TGFβ2mediated TGFβmediated Conclusions acidinduced induced CD44/TGFβ2 CD44TGFβ2 CDTGFβ CD44/TGF CD44/TGF-β (HLEB 30 1. (1 CD44/TGFβ CD44TGFβ CD44TGF CDTGF 3 (

RESUMO: Dentre as neoplasias hematopoiéticas, as leucemias são originadas pelas células mieloides ou linfoides na medula óssea, podendo ser de forma aguda, com um pior prognóstico, principalmente, acometendo gatos com leucemia felina (FeLV positivos) ou, de forma crônica, que apresentam um prognostico reservado, especialmente em gatos idosos. As leucemias possuem várias classificações, cujas diferenciações irão depender de exames complementares como hemograma, mielograma, citologia e citometria de fluxo, importantes para a caracterização e para o melhor tratamento do animal. RESUMO hematopoiéticas óssea aguda prognóstico principalmente FeLV positivos crônica reservado idosos classificações hemograma mielograma fluxo animal

ABSTRACT: Among the hematopoietic neoplasms, leukemias are caused by myeloid or lymphoid cells in the bone marrow, which can either be acute with an unfavorable prognosis and mostly affect cats that tested positive for feline viral leukemia (FeLV) or chronic in older cats that have a poor prognosis. Leukemias have several classifications in which the differentiation depends on complementary tests, such as blood profile, myelogram, cytology. and flow cytometry, which help determine the best treatment for the animal. ABSTRACT neoplasms marrow FeLV (FeLV tests profile myelogram cytology cytometry animal

RESUMO A aplicação de esgoto sanitário utilizando o gotejamento subsuperficial contribui para mitigar os problemas atuais de disponibilidade de água e produção de alimento. Objetivou-se avaliar o feijão-caupi recebendo esgoto sanitário por gotejadores operando com diferentes vazões e profundidades. Os gotejadores operaram com as vazões de 1,6 e 3,8 L h-1 e foram instalados nas profundidades de 0, 5, 10, 15, 20, 25 e 30 cm. O feijão-caupi foi cultivado em vasos de 65 L preenchidos com solo argiloso em casa de vegetação. Utilizou-se esgoto sanitário sintético e a quantidade aplicada nos vasos foi baseada no elemento limitante, que no caso foi o nitrogênio, para o cultivo do feijão-caupi. O manejo da irrigação foi realizado com sondas de TDR. Avaliou-se a germinação e as respostas fisiológicas e morfologia das raízes do feijão-caupi. A ascensão capilar, quando a água foi aplicada em profundidade, não foi suficiente para umedecer de maneira uniforme a superfície do solo. As variáveis de germinação apresentaram um decréscimo em função do aumento das profundidades de instalação dos gotejadores. A distribuição da água com o aumento da profundidade dos emissores foi responsável pelos prejuízos nas respostas fisiológicas e morfológicas das raízes feijão-caupi. As vazões dos gotejadores não afetaram a germinação do feijão-caupi. Utilizar o sistema de irrigação por gotejamento subsuperficial não foi apropriado para o feijão-caupi nas condições deste estudo. alimento Objetivouse Objetivou se feijãocaupi feijão caupi 16 1 6 1, 38 3 8 3, h1 h h- 0 5 10 15 20 2 cm vegetação Utilizouse Utilizou limitante nitrogênio feijãocaupi. caupi. TDR Avaliouse Avaliou capilar estudo

ABSTRACT The application of sanitary sewage using subsurface drip irrigation contributes to mitigating the current problems of water availability and food production. The aim in this study was to evaluate cowpea receiving sanitary sewage through drippers operating at different flow rates and depths. The drippers operated at flow rates of 1.6 and 3.8 L h-1 and were installed at depths of 0, 5, 10, 15, 20, 25, and 30 cm. Cowpea was grown in 65 L pots filled with sandy clay soil in a greenhouse. Synthetic sanitary sewage was used and the amount applied to the pots was based on the limiting element, which in this case was nitrogen, for growing cowpea. Irrigation was managed using TDR probes. Germination and the physiological responses and morphology of cowpea roots were assessed. Capillary rise, when water was applied in subsurface, was not sufficient to evenly moisten the soil surface. The germination variables decreased as a result of the increase in drip installation depths. The distribution of water as the depth of the emitters increased was responsible for the damage to the physiological and morphological responses of cowpea roots. The flow rates of the drippers did not affect the germination of cowpea. Using the subsurface drip irrigation system was not appropriate for growing cowpea under the conditions of this study. production 16 1 6 1. 38 3 8 3. h1 h h- 0 5 10 15 20 25 cm greenhouse element nitrogen probes assessed rise surface 2

Resumo O presente estudo avaliou a incorporação de finos de rocha granítica (GRP) e areia artificial (MS) em argamassas auto-adensáveis (SCM), visando adequação das características reológicas. Foram analisadas SCMs com relações água/cimento de 0,42, 0,46 e 0,63, teores de GRP de 17%, 20% e 40%, respetivamente, e substituição volumétrica de 50% da areia natural por MS. Para avaliar o desempenho das SCMs, foram realizados ensaios de mini V-Funnel e mini Slump, além da caraterização físico-mecânica. A distância de separação interpartículas e a espessura máxima da pasta também foram analisadas e concluiu-se que o teor de GRP é o parâmetro de maior influência no consumo de superplastificante e no tempo de escoamento das misturas. Com a incorporação de GRP, a viscosidade da argamassa foi ajustada sem a necessidade de adição de agentes modificadores de viscosidade, aumentando o tempo de escoamento, enquanto a substituição parcial de NS por MS resultou numa diminuição deste parâmetro. Além disso, a incorporação simultânea de GRP e MS aumentou a densidade das misturas, promovendo aumento da resistência à compressão em até 29% e da resistência à tração na flexão em até 19%, apesar da redução de cerca de 40% do volume de cimento. (GRP (MS autoadensáveis auto adensáveis SCM, SCM , (SCM) reológicas águacimento água cimento 042 0 42 0,42 046 46 0,4 063 63 0,63 17 17% 20 40 respetivamente 50 VFunnel V Funnel Slump físicomecânica. físicomecânica físico mecânica. mecânica físico-mecânica concluiuse concluiu se misturas disso 29 19 19% (SCM 04 4 0, 06 6 0,6 1 2 5

Abstract The study assessed the use of granitic rock powder (GRP) and manufactured sand (MS) in self-compacting mortars (SCM) to achieve desired rheological properties. Different water/cement ratios of 0.42, 0.46, and 0.63 were used with partial replacement of cement volume by GRP at levels of 17%, 20%, and 40%. Additionally, a volumetric replacement of 50% natural sand with MS was studied. The performance of SCMs was evaluated using mini V-Funnel and mini Slump Tests, as well as physical-mechanical characterization. It was found that GRP content significantly influenced superplasticizer consumption and flow time without requiring additional viscosity modifiers while incorporating MS decreased flow time parameter. Furthermore, simultaneous incorporation of GRP and MS increased mixture density by up to 14% improved compressive strength by up to 29%, and flexural tensile strength by up to19%. (GRP (MS selfcompacting self compacting SCM (SCM properties watercement water 042 0 42 0.42 046 46 0.46 063 63 0.6 17 17% 20 20% 40 40% Additionally 50 studied VFunnel V Funnel Tests physicalmechanical physical mechanical characterization parameter Furthermore 14 29 29% to19 to19% 04 4 0.4 06 6 0. 1 2 5 to1

RESUMEN Este trabajo tiene por objetivo presentar un método de evaluación y análisis de la cadena de suministro como estructura organizativa cuya salida es un producto de calidad y alta demanda en el sector agropecuario. Se presentan definiciones, así como los elementos que definen a la cadena de suministro como estructura organizativa. Se brinda un método de evaluación y análisis, estableciendo sus dimensiones de trabajo y las etapas para su desarrollo. Su aplicación práctica permite realizar una síntesis de los factores que entorpecen y/o facilitan la efectividad de la cadena en el contexto de la economía cubana. Se expone el resultado del proyecto de investigación «Mejorar la cadena de suministro de productos cárnicos del sector agroalimentario para la exportación en fronteras. Código: PN131SS014-011», perteneciente a un Programa Nacional de Ciencia y Técnica.

ABSTRAC This work aims to present a method of evaluation and analysis of the supply chain as an organizational structure whose output is a quality product and high demand in the agricultural sector. Definitions are presented, as well as the elements that define the supply chain as an organizational structure. An evaluation and analysis method is provided, establishing its dimensions of work and the stages for its development. Its practical application allows a synthesis of the factors that hinder and/or facilitate the effectiveness of the chain in the context of the Cuban economy. The result of the research project «Improving the supply chain of meat products in the agri-food sector for export at borders Code PN131SS014-011», belonging to add files National Program of Science and Technique

Summary: Avocado is a fruit (Persea americana), that has increased its consumption worldwide. Mexico is the main producer and exporter of avocado in the world, representing more than 30% of the world harvest, of which 73.2% of the total avocado production in Mexico was concentrated in the state of Michoacán. In the state of Michoacán, there is a constant production of avocado fruit throughout the year, but this production is affected by the variation in climate, water management and flowering type, putting agricultural production and sustainable development at risk. The objective of this study was to evaluate the climatic effect, water management, and flowering type on fruit nutrient content in six orchards in the state of Michoacan. Six orchards were evaluated in the towns of Basilia, Cheranguerán, Tecario, Araparicuaro, Peribán and Patamburu in the state of Michoacan, which represent 90% of the climate types (TC), predominant in the state (ACW1, ACW2 and CW1)., with 100% of the water regime (irrigated and seasonal, RH) and 97% of the type of fruit produced (loca and normal, FF). The dry matter contents and total mineral content of the fruit were evaluated. The arrangement of treatments was a factorial [A= water regime (RH); B= type of climate (TC); C= floral flow (FF)], distributed completely randomly with 20 repetitions taking each tree as a repetition. The results indicated that the dry matter content is affected by TC, but not by RH and FF, FF affects the nutritional content more than TC and RH. The type of climate is the factor with the least impact on the mineral content of the fruit. In floral flow, the total nutritional content was significant in four elements with higher values for N, Cl, and Zn in crazy fruit and Na in normal fruit.

Resumen: El aguacate (Persea americana), es un fruto que ha incrementado su consumo a nivel mundial. México es el principal productor y exportador de aguacate en el mundo que representa más del 30% de la cosecha mundial, del cual el 73.2% de la producción total de aguacate en México se concentra en el estado de Michoacán, pero dicha producción es afectada por la variación del clima, poniendo en peligro la producción agrícola y el desarrollo sostenible. El objetivo del fue evaluar el efecto climático, manejo de agua y el tipo de floración sobre el contenido nutrimental del fruto en seis huertas del estado de Michoacán. Se evaluaron seis huertos de las localidades de la Basilia, Cheranguerán, Tecario, Araparicuaro, Peribán y Patamburu del estado de Michoacan, que representan un 90% de los tipos de clima (TC), predominantes en el estado (ACW1, ACW2 y CW2), con un 100% del régimen de agua (riego y temporal, RH) y un 97% del tipo de fruto producido (loca y normal, FF). Se evaluaron los contenidos de materia seca y el contenido mineral total del fruto. El arreglo de tratamientos fue una factorial [A= régimen hídrico (RH); B= tipo de clima (TC); C= flujo floral (FF)], distribuido completamente al azar con 20 repeticiones tomando cada árbol como una repetición. Los resultados indicaron que el contenido de materia seca es afectado por TC, pero no por RH y FF, FF afecta más el contenido nutricional que TC y RH. El tipo de clima es el factor de menor impacto en el contenido mineral del fruto. En flujo floral el contenido nutricional total fue significativo en cuatro elementos con mayor valor para N, Cl, y Zn en fruto loca y Na en fruto normal.

Resumen:El presente estudio analiza los principales factores que tienen influencia en el consumo de agua potable, así como los patrones de consumo horario del recurso hídrico en zonas residenciales de la parroquia El Rosario. Se aplicó un estudio de caracterización urbanística por medio de fichas técnicas y análisis estadístico que denotan 3 estratificaciones en el sector (B, C y D). Se aplicaron encuestas para conocer factores que inciden en el consumo de agua potable y se realizó el registro horario de lecturas de 219 medidores de agua durante 24 horas los 7 días de la semana. El consumo de agua potable registrado presenta incrementos que tienen dependencia a factores como el número de usuarios que disponen del servicio, tipo de residencia, unidades sanitarias en uso y otras características externas. Se obtuvieron curvas de consumo horarias para cada red de distribución y los coeficientes de variación de consumo horario máximos y mínimos. Los caudales máximos ocurren a las 13h00 con 94.76 l/h y a las 19h00 con 73.46 l/h, el coeficiente máximo de variación de consumo horario (Kh_max) es de 3.44, valor que sobrepasa lo establecido en la normativa nacional vigente CPE INEN 5 (1992); los valores de los coeficientes máximos de cada barrio se presentan mediante digitalización georreferenciada.

Abstract:This study analyzes the main factors that influence drinking water consumption, as well as the hourly water consumption patterns in residential areas of El Rosario parish. A study of urban characterization was applied by means of technical data sheets and statistical analysis that showed 3 stratifications in the sector (B, C and D). Surveys were conducted to determine the factors that affect drinking water consumption, and an hourly record was made of readings of 219 water meters 24 hours a day, 7 days a week. The consumption of drinking water recorded showed increases that depend on factors such as the number of users who have the service, type of residence, sanitary units in use and other external characteristics. Hourly consumption curves were obtained for each distribution network and the coefficients of variation of maximum and minimum hourly consumption. The maximum flows occur at 13h00 with 94.76 l/h and at 19h00 with 73.46 l/h, the maximum coefficient of variation of hourly consumption (Kh_max) is 3.44, a value that exceeds that established in the current national regulation CPE INEN 5 (1992); the values of the maximum coefficients of each neighborhood are presented through georeferenced digitalization.

Abstract Introduction: Evidence suggests that herbivores, such as peccaries, shape vegetation structure and diversity through predation, trampling, dispersal, and rooting behavior. Objective: To evaluate the impact of peccaries (Dycotiles tajacu) on the understory vegetation of the tropical rainforest in the Nogal-La Selva Local Biological Corridor, Costa Rica, comparing a site with the absence of peccaries to another with the presence of these animals. Methodology: From June to November 2021, 20 experimental exclusions and 20 free access plots, each measuring 2 m2 were used to quantify herbivory, the number of leaf blades, damaged leaves, healthy leaves, sapling height, and fallen biomass at both sites. Results: A higher sapling density was found in the Nogal Reserve, but a lower sapling diversity, while in La Selva there was a higher sapling diversity, but a lower density of seedlings. Herbivory and sapling height in La Selva exceeded those in Nogal. The exclusion of peccaries reduced seedling damage but did not affect the dynamics of fallen biomass. Conclusion: For the design, implementation, and evaluation of the effectiveness of biological corridors, it is crucial to consider plant-animal interactions to enhance the flow of ecological processes through functional and structural connectivity, analyzed from interactions such as those presented in this paper.

Resumen Introducción: Existe evidencia que herbívoros, como los saínos, dan forma a la estructura y diversidad de la vegetación a través del comportamiento de depredación, pisoteo, dispersión y enraizamiento. Objetivo: Evaluar el impacto de los saínos (Dycotiles tajacu) en la vegetación del sotobosque del bosque tropical húmedo en el Corredor Biológico Local Nogal-La Selva, Costa Rica, en un sitio con ausencia y en otro con presencia de saínos. Métodos: De junio a noviembre de 2021 se utilizaron 20 exclusiones experimentales y 20 parcelas de acceso libre de 2 m2, se cuantifico la herbivoría, número de láminas foliares, hojas dañadas, hojas sanas, altura de brinzales y biomasa caída en ambos sitios. Resultados: Se encontró una mayor densidad de brinzales en Reserva Nogal pero una menor diversidad, contrario en La Selva donde se encontró una mayor diversidad de brinzales, pero una menor densidad de plántulas. La herbivoría y la altura de brinzales en La Selva fue mayor que en Nogal. La exclusión de los saínos disminuyó el daño a las plántulas, pero no afectó la dinámica de la biomasa caída. Conclusión: Es necesario contemplar para el diseño, implementación y evaluación de la efectividad de corredores biológicos, las interacciones planta-animal, para potencializar el flujo de procesos ecológicos mediante la conectividad funcional y estructural, analizada a partir de interacciones como las presentadas en este trabajo.

Abstract: The integration of electric mobility is real nowadays. Government policies increasingly focus on encouraging the use of electric vehicles (EVs); particularly, replacing conventional public mobility with electric buses. An adequate energy management of electric bus systems must optimize resource usage, and extend the lifespan of transport units and charging stations, while providing quality service to users. The performance of the EV transport system depends on various deterministic and stochastic variables that need to be accurately simulated to analyze its behavior as closely as possible to real conditions. In this study, a model for the energy analysis of EVs has been implemented, by using the software SUMO (Simulation of Urban Mobility) and applying it to public transport routes in Quito, Ecuador. Through the executed simulations, energy consumption has been estimated and, a sensitivity analysis of the energy consumption of electric bus units to the variations in relevant parameters in the energy management of public transport systems, has been carried out. The impact of certain parameters on energy consumption has been verified, by indicating a significant decrease in the autonomy of EVs on the simulated route under very similar to reality operating conditions. For study case, autonomy reduction of electric bus is around 27% in comparison with manufacturer's specifications. The analysis of energy performance under various traffic scenarios will allow future optimizations of the electric public transport system operation, since traffic levels, variable traffic flow, and pronounced changes in terrain elevation, are considered.

Resumen: La integración de la movilidad eléctrica es una realidad en el mundo actual, las políticas gubernamentales se enfocan cada vez más en incentivar el uso de vehículos eléctricos (VE) particulares, pero, principalmente en reemplazar la movilidad pública convencional por el uso de buses eléctricos. Una adecuada gestión energética de los sistemas de buses eléctricos debe optimizar el uso de recursos, prolongando la vida útil de las unidades de transporte, estaciones de carga y brindando siempre un servicio de calidad a sus usuarios. El desempeño del sistema de transporte mediante VE depende de diversas variables determinísticas y estocásticas que necesitan ser debidamente simuladas a fin de analizar su comportamiento, lo más cercano a las condiciones reales. En el presente trabajo, mediante la aplicación del software SUMO (Simulation of Urban Mobility), se ha implementado un modelo para el análisis energético de VE y se lo ha aplicado en rutas de transporte público en Quito-Ecuador. A partir de las simulaciones realizadas se ha estimado el consumo energético y se ha efectuado un análisis de sensibilidad del consumo energético de las unidades de buses eléctricos ante variaciones de parámetros relevantes en la gestión energética de sistemas de transporte público. Para el caso de estudio se comprueba la reducción de la autonomía del bus eléctrico en 27% de acuerdo con la información del fabricante. El análisis del desempeño energético realizado bajo diversos escenarios de tráfico permitirá en futuros trabajos optimizar la operación de los sistemas de transporte público eléctrico, considerando niveles de tráfico, flujo vehicular variable y pronunciados desniveles en su relieve.

ABSTRACT Streamflow data from gauging stations are essential for effective water resources management. However, some regions in Brazil lack the necessary data. Hydrological regionalization is an alternative technique for obtaining data such regions. However, not all regions in Brazil have defined hydrological regionalization models, including the state of Goiás and the Brazilian Federal District. The objective of this study was to develop a hydrological regionalization methodology based on the separation of hydrologically homogeneous regions and multiple linear regression, using a Geographic Information System (GIS) program. Historical series data were used to calculate reference flows with 90 or 95% duration over time in the watercourse (Q90 and Q95) and the mean flow (Ǭ). Rain gauge station data were used to calculate the mean annual rainfall in each watershed through spatial interpolation by ordinary kriging. Subsequently, the physiographic characteristics of each watershed were calculated. The hydrologically homogeneous regions were delimited based on these data using cluster analysis, which identified seven hydrologically homogeneous regions in Goiás, two of them belonging to the Federal District. Multiple regression allowed the development of seven regionalization models. Models for regions 1, 3, 4, 5, and 7 showed better performance. management However models District GIS (GIS program 9 95 Q90 Q (Q9 Q95 Ǭ. Ǭ . (Ǭ) kriging Subsequently calculated analysis 1 3 4 5 performance Q9 (Q (Ǭ

RESUMO Para gerenciar os recursos hídricos de forma eficaz, os dados de vazão das estações hidrométricas são cruciais. No entanto, no Brasil, algumas regiões não dispõem dos dados necessários. A regionalização hidrológica é uma técnica alternativa para obter dados para essas regiões. Infelizmente, nem todas as regiões do Brasil têm modelos de regionalização hidrológica definidos, incluindo Goiás e o Distrito Federal. O objetivo deste estudo foi desenvolver uma metodologia de regionalização hidrológica, baseada na separação de regiões hidrologicamente homogêneas e na regressão multivariada, utilizando o programa Sistema de Informações Geográficas (SIG). Com base na série histórica, foram calculadas as vazões de referência com 90 ou 95% de permanência ao longo do tempo no curso d’água (Q90 e Q95) e a vazão média (Ǭ). Com os dados das estações pluviométricas, foi calculada a precipitação média anual de cada uma das bacias hidrográficas fluviométricas, por meio de interpolação espacial por krigagem ordinária. Posteriormente, foram calculadas as características fisiográficas de cada bacia. Com esses dados, as regiões hidrologicamente homogêneas foram delimitadas por meio da análise de agrupamento. A análise de cluster identificou sete regiões hidrologicamente homogêneas em Goiás, sendo que duas delas pertencem ao Distrito Federal. A regressão multivariada levou ao desenvolvimento de sete modelos de regionalização. Os modelos para as regiões 1, 3, 4, 5 e 7 apresentaram melhor desempenho. eficaz cruciais entanto necessários Infelizmente definidos Federal SIG. SIG . (SIG) histórica 9 95 dágua d água Q90 Q (Q9 Q95 Ǭ. Ǭ (Ǭ) pluviométricas fluviométricas ordinária Posteriormente bacia agrupamento 1 3 4 desempenho (SIG Q9 (Q (Ǭ

O campo digital pode ser visto de modo polissêmico, com dinâmicas de controle e vigilância, mas também como espaço criativo para emergência de novas sociabilidades. O presente trabalho enfatiza a segunda dimensão, investigando as contribuições das culturas digitais para o fortalecimento do campo social da Literatura de Cordel (LC) em suas articulações em redes, e na difusão e visibilidade dos seus atores sociais. A Literatura de Cordel  uma forma de poesia popular tradicional do Nordeste do Brasil, tem exibida uma nova vida nas mídias sociais. Muitos cordelistas divulgam seus projetos no Instagram, Facebook e YouTube para compartilhar seus legados e alcançar maior número de pessoas possível. Nessas mídias sociais circulam versos tradicionais de cordel, mas também adaptações para o ambiente digital, como vídeos de cordelistas recitando poemas, e até mesmo interações em tempo real com os seguidores. Isso tem contribuído para a preservação da tradição, da memória e identidade da LC e para a sua divulgação. O artigo investe na abordagem qualitativa da etnografia com observação participante para analisar os fluxos de interações de alguns cordelistas nas mídias sociais. Como resultado parcial, observa-se que as mídias sociais oferecem plataformas potentes para a divulgação e promoção de obras de Literatura de Cordel; bem como ambiente para encontros dos poetas e cordelistas em geral.

The digital field can be seen in a polysemic way, with dynamics of control and surveillance, but also as a creative space for emerging new sociabilities. This paper emphasizes the second dimension, investigating the contributions of digital cultures to strengthening the social field of Cordel Literature (LC) in its articulations in networks, and in the dissemination and visibility of its social actors. LC, a traditional form of popular poetry from the northeast of Brazil, has been given a new lease of life on social media. Many poets publicize their projects on Instagram, Facebook, and YouTube to share their legacies and reach as many people as possible. In these social media, traditional verses of Literatura de Cordel circulate, but also adaptations for the digital environment, such as videos of poets reciting poems, and even real-time interactions with followers. This has contributed to preserving the tradition, memory, and identity of the LC and its dissemination. This is a qualitative study that uses ethnography and participant observation to analyze the flow of interactions between some poets on social media. As a partial result, it was observed that social media offer powerful platforms for the dissemination and promotion of works of Cordel Literature, as well as an environment for meetings between poets and cordelistas in general.

RESUMEN En el riego por goteo, el volumen de suelo mojado representa la cantidad de agua almacenada y su extensión, profundidad y diámetro deben coincidir con el sistema radicular de la planta y el espaciamiento entre emisores, sin embargo, en la práctica del diseño, no se considera el radio de humedecimiento efectivo que alcanza estos dispositivos. Se comparan los resultados del diseño agronómico obtenido con procedimientos concebidos para condiciones específicas, y se identifican los efectos que sobre los parámetros de explotación ejerce los parámetros de diseño. Se utilizó el método de análisis y síntesis, a partir de la comparación de tres procedimientos de diseño, uno empleando los criterios expuestos por Arapa /2002, el segundo, teniendo en cuenta los criterios expuestos por Cruz-Batista et al. /2015 y el tercero utilizando el procedimiento alternativo que se aplica en las UEB de Consultoría y Diseño de la ENPA. Los resultados mostraron la viabilidad de utilizar modelos experimentales para al diseño de sistemas de riego por goteo, ante la imposibilidad de realizar pruebas de campo, estas herramientas permiten prever el avance lateral y vertical del agua debajo de los emisores. Se constató que la transferencia de humedad debajo de los emisores, es función del volumen de agua aplicada, el caudal del emisor, la conductividad hidráulica saturada, el contenido de humedad inicial y residual y de limo en el suelo. Esta comparación permite afirmar la validez de utilizar modelos de simulación para estimar la separación entre emisores necesarios para humedecer el volumen de suelo requerido.

ABSTRACT In drip irrigation, the volume of wetted soil represents the amount of water stored and its shape and dimensions should correspond to the plant root system and the spacing between emitters, however in design practice, the effective wetting radius reached by these devices is not considered. The results of the agronomic design obtained with different design procedures are compared and their effects on operating parameters are identified. The analysis and synthesis method was used, based on the comparison of three design procedures, one using the criteria set out by Arapa /2002, the second, taking into account the criteria set out by Cruz-Batista et al. /2015 and the third using the alternative procedure applied in the UEB Consultancy and Design of the ENPA. The results showed the feasibility of using experimental models for the design of drip irrigation systems, given the impossibility of carrying out field tests; these tools make it possible to predict the lateral and vertical advance of water under the emitters. It was found that moisture transfer under the emitters is a function of the volume of water applied, the flow rate of the emitter, the saturated hydraulic conductivity, the initial and residual moisture content and the silt content of the soil. The comparison allowed affirming the validity of using simulation models to estimate the emitter spacing necessary to wet the required soil volume.

RESUMEN El Instituto Nacional de Recursos Hidráulicos promueve la gestión eficiente del agua en las empresas agropecuarias, sobre la base de aplicar resultados de investigaciones científicas. A pesar de la inversión realizada en la Empresa Agropecuaria Laguna Blanca, aún persisten dificultades para hacer sostenible las producciones agrícolas, debido a la baja agroproductividad de los suelos y el descenso de la eficiencia en el uso del agua de riego, por ello se infiere la necesidad de actualizar, desde el diseño de las instalaciones, los parámetros de explotación de los sistemas de riego con máquinas de Pivote Central y Enrolladores. Se aborda, a partir de la solución de dos casos de estudios reales, los efectos negativos de la presencia de sales en la solución del suelo, sobre el rendimiento de los cultivos y en la sostenibilidad de las fuentes de abasto implicadas en la actividad del riego. Se empleó el método hipotético - deductivo, para validar la conveniencia de considerar en la etapa de diseño, la salinidad en el extracto de saturación del suelo. Como resultado se identificó el caudal medio de bombeo, como el parámetro de diseño que influye significativamente en la explotación de estas máquinas de riego; el incremento de este parámetro infiere la necesidad de establecer sistemas de drenaje parcelario para disminuir los efectos de la saturación de los suelos y la presencia de sales en la solución del suelo.

ABSTRACT The National Institute of Water Resources promotes efficient water management in agricultural enterprises, based on the application of scientific research results. In spite of the investment made in the Laguna Blanca Agricultural Enterprise, there are still difficulties in making agricultural production sustainable, due to the low agro-productivity of the soils and the decrease in the efficiency of irrigation water use. For this reason, it is necessary to update, from the design of the installations, the operating parameters of the irrigation systems with centre pivot and reel machines. Based on the solution of two real case studies, the negative effects of the presence of salts in the soil solution on crop yields and on the sustainability of the supply sources involved in the irrigation activity are addressed. The hypothetical-deductive method was used to validate the convenience of considering salinity in the soil saturation extract at the design stage. As a result, the average pumping flow rate was identified as the design parameter that significantly influences the operation of these irrigation machines; the increase of this parameter infers the need to establish plot drainage systems to reduce the effects of soil saturation and the presence of salts in the soil solution.