Resumo O estudo realizou uma revisão do perfil parasitológico de vegetais, de 2001 a 2021, considerando o tipo, consumo e cultivo, global. Foram pesquisadas as bases MEDLINE, SciELO, Web of Science, Science Direct e Scopus com os termos "Detection OR Prevalence OR Incidence OR occurrence OR contamination AND vegetable OR fruit AND Helminth OR egg OR Parasite OR cysts OR protozoa". Foram encontrados 16.600 artigos, 117 dos quais foram revisados. Das 391.291 amostras, 3,85% (15.095) estavam contaminadas por parasitos. Entre as positivas, 30,10% (4.543/15.095) continham enteroparasitos comumente de origem humana e 58,78% (8.873/15.095) eram originadas de mercados. Poucos artigos mencionaram o tipo de cultivo, mas entre esses, o convencional apresentou maior contaminação (42,34%; 224/529). Vegetais herbáceos foram os mais contaminados (56,84%; 8.580/15.095). Ascaris lumbricoides foi encontrado em 10,16% (1.535/15.095) das amostras. Alface foi o vegetal mais contaminado (20,43%; 3.084/15.095). vegetais 200 2021 cultivo global MEDLINE SciELO Detection protozoa. protozoa . protozoa" 16600 16 600 16.60 11 revisados 391291 391 291 391.29 amostras 385 3 85 3,85 15.095 15095 15 095 (15.095 parasitos positivas 3010 30 10 30,10 4.543/15.095 454315095 4 543 (4.543/15.095 5878 58 78 58,78 8.873/15.095 887315095 8 873 (8.873/15.095 mercados esses 42,34% 4234 42 34 (42,34% 224/529. 224529 224/529 224 529 224/529) 56,84% 5684 56 84 (56,84% 8.580/15.095. 858015095 8.580/15.095 580 8.580/15.095) 1016 10,16 1.535/15.095 153515095 1 535 (1.535/15.095 20,43% 2043 20 43 (20,43% 3.084/15.095. 308415095 3.084/15.095 084 3.084/15.095) 202 1660 60 16.6 39129 39 29 391.2 38 3,8 15.09 1509 09 (15.09 301 30,1 4.543/15.09 45431509 54 (4.543/15.09 587 5 7 58,7 8.873/15.09 88731509 87 (8.873/15.09 42,34 423 (42,34 22452 224/52 22 52 56,84 568 (56,84 85801509 8.580/15.09 101 10,1 1.535/15.09 15351509 53 (1.535/15.09 20,43 204 2 (20,43 30841509 3.084/15.09 08 166 6 16. 3912 391. 3, 15.0 150 0 (15.0 30, 4.543/15.0 4543150 (4.543/15.0 58, 8.873/15.0 8873150 (8.873/15.0 42,3 (42,3 2245 224/5 56,8 (56,8 8580150 8.580/15.0 10, 1.535/15.0 1535150 (1.535/15.0 20,4 (20,4 3084150 3.084/15.0 15. (15. 4.543/15. 454315 (4.543/15. 8.873/15. 887315 (8.873/15. 42, (42, 224/ 56, (56, 858015 8.580/15. 1.535/15. 153515 (1.535/15. 20, (20, 308415 3.084/15. (15 4.543/15 45431 (4.543/15 8.873/15 88731 (8.873/15 (42 (56 85801 8.580/15 1.535/15 15351 (1.535/15 (20 30841 3.084/15 (1 4.543/1 4543 (4.543/1 8.873/1 8873 (8.873/1 (4 (5 8580 8.580/1 1.535/1 1535 (1.535/1 (2 3084 3.084/1 ( 4.543/ 454 (4.543/ 8.873/ 887 (8.873/ 858 8.580/ 1.535/ 153 (1.535/ 308 3.084/ 4.543 45 (4.543 8.873 88 (8.873 8.580 1.535 (1.535 3.084 4.54 (4.54 8.87 (8.87 8.58 1.53 (1.53 3.08 4.5 (4.5 8.8 (8.8 8.5 1.5 (1.5 3.0 4. (4. 8. (8. 1. (1. 3. (8

Abstract The study conducted a review of the parasitological profile of vegetables from 2001 to 2021, considering the type, consumption, and cultivation, globally. The databases searched included MEDLINE, SciELO, Web of Science, Science Direct, and Scopus using the terms "Detection OR Prevalence OR Incidence OR occurrence OR contamination AND vegetable OR fruit AND Helminth OR egg OR Parasite OR cysts OR protozoa". A total of 16,600 articles were found, 117 of which were reviewed. Of the 391,291 samples, 3.85% (15,095) were contaminated by parasites. Among those positive, 30.10% (4,543/15,095) contained enteroparasites commonly of human origin and 58.78% (8,873/15,095) came from markets. Few articles mentioned the cultivation type, but among those, conventional cultivation showed more contamination (42.34%; 224/529). Herbaceous vegetables were the most contaminated (56.84%; 8,580/15,095. Ascaris lumbricoides was found in 10.16% (1,535/15,095) of the samples. Lettuce was the most contaminated (20.43%; 3,084/15,095). 200 2021 type consumption globally MEDLINE SciELO Direct Detection protozoa. protozoa . protozoa" 16600 16 600 16,60 11 reviewed 391291 391 291 391,29 samples 385 3 85 3.85 15,095 15095 15 095 (15,095 parasites positive 3010 30 10 30.10 4,543/15,095 454315095 4 543 (4,543/15,095 5878 58 78 58.78 8,873/15,095 887315095 8 873 (8,873/15,095 markets 42.34% 4234 42 34 (42.34% 224/529. 224529 224/529 224 529 224/529) 56.84% 5684 56 84 (56.84% 858015095 580 8,580/15,095 1016 10.16 1,535/15,095 153515095 1 535 (1,535/15,095 20.43% 2043 20 43 (20.43% 3,084/15,095. 308415095 3,084/15,095 084 3,084/15,095) 202 1660 60 16,6 39129 39 29 391,2 38 3.8 15,09 1509 09 (15,09 301 30.1 4,543/15,09 45431509 54 (4,543/15,09 587 5 7 58.7 8,873/15,09 88731509 87 (8,873/15,09 42.34 423 (42.34 22452 224/52 22 52 56.84 568 (56.84 85801509 8,580/15,09 101 10.1 1,535/15,09 15351509 53 (1,535/15,09 20.43 204 2 (20.43 30841509 3,084/15,09 08 166 6 16, 3912 391, 3. 15,0 150 0 (15,0 30. 4,543/15,0 4543150 (4,543/15,0 58. 8,873/15,0 8873150 (8,873/15,0 42.3 (42.3 2245 224/5 56.8 (56.8 8580150 8,580/15,0 10. 1,535/15,0 1535150 (1,535/15,0 20.4 (20.4 3084150 3,084/15,0 15, (15, 4,543/15, 454315 (4,543/15, 8,873/15, 887315 (8,873/15, 42. (42. 224/ 56. (56. 858015 8,580/15, 1,535/15, 153515 (1,535/15, 20. (20. 308415 3,084/15, (15 4,543/15 45431 (4,543/15 8,873/15 88731 (8,873/15 (42 (56 85801 8,580/15 1,535/15 15351 (1,535/15 (20 30841 3,084/15 (1 4,543/1 4543 (4,543/1 8,873/1 8873 (8,873/1 (4 (5 8580 8,580/1 1,535/1 1535 (1,535/1 (2 3084 3,084/1 ( 4,543/ 454 (4,543/ 8,873/ 887 (8,873/ 858 8,580/ 1,535/ 153 (1,535/ 308 3,084/ 4,543 45 (4,543 8,873 88 (8,873 8,580 1,535 (1,535 3,084 4,54 (4,54 8,87 (8,87 8,58 1,53 (1,53 3,08 4,5 (4,5 8,8 (8,8 8,5 1,5 (1,5 3,0 4, (4, 8, (8, 1, (1, 3, (8

Resumo Parasitos zoonóticos caninos têm sido reconhecidos como um importante problema de saúde pública, especialmente em países em desenvolvimento com condições socioambientais vulneráveis. No contexto de “One Health” o objetivo deste trabalho foi avaliar o domínio animal pela avaliação de enteroparasitas zoonóticos caninos associados a um Índice de Vulnerabilidade Parasitária (PVI) em comunidades periféricas (PC) e urbanas (UC) de Mar del Plata cidade. O PVI foi elaborado em trabalho anterior como indicador de vulnerabilidade a doenças parasitárias, em relação às condições socioambientais levantadas nas comunidades nas seguintes dimensões: moradia, saneamento, higiene, educação e trabalho. Um estudo coproparasitológico foi realizado para estabelecer a presença e a riqueza de parasitas caninos no ambiente em ambas as comunidades. O PC evidenciou domicílios com maior número médio de amostras fecais caninas (CFS) com parasitas do que o UC, apresentando também a maior riqueza específica parasitária com helmintos e protozoários. As frequências de Ancilostomídeos, Capillariids e Toxocara canis foram maiores no PC, mas as frequências de Trichuris vulpis e o teste de coproantígeno positivo para detectar Echinococcus granulosus, foram semelhantes entre as comunidades. O CP evidenciou associação com variáveis relacionadas à posse de cães (ausência de atendimento veterinário e não vermifugação adequada), com a presença de SFC com parasitas e o teste de coproantígeno positivo. Famílias com PVI médio e alto do PC evidenciaram forte associação com a presença de SFC com parasitos e também com alta riqueza parasitária, enquanto famílias da UC com baixo PVI evidenciaram SFC sem parasitos. Esses resultados revelaram um cenário vulnerável para a permanência e transmissão de parasitoses zoonóticas caninas na maioria das famílias do PC, destacando o valor das características socioambientais como preditores de parasitoses.

Resumen Los parásitos zoonóticos caninos han sido reconocidos como un importante problema de salud pública, especialmente en países en desarrollo con condiciones socioambientales vulnerables. En el contexto de “Una Salud” el objetivo de este trabajo fue evaluar el dominio animal mediante la evaluación de enteroparásitos zoonóticos caninos asociados a un Índice de Vulnerabilidad Parasitaria (IVP) en comunidades periféricas (CP) y urbanas (CU) de la ciudad de Mar del Plata. El IPV fue elaborado en un trabajo anterior como un indicador de vulnerabilidad de enfermedades parasitarias, en relación a las condiciones socioambientales relevadas en las comunidades sobre las siguientes dimensiones: vivienda, saneamiento, higiene, educación y trabajo. Se realizó un estudio coproparasitológico para establecer la presencia y la riqueza de parásitos caninos en el ambiente de ambas comunidades. La CP evidenció hogares con mayor número medio de muestras fecales caninas (MFC) con parásitos que la CU, mostrando también la mayor riqueza específica parasitaria con helmintos y protozoos. Las frecuencias de Ancylostomids, Capillariids y Toxocara canis fueron mayores en la CP, pero las frecuencias de Trichuris vulpis y la prueba de coproantígeno positivo para detectar Echinococcus granulosus, fueron similares entre comunidades. La CP evidenció asociación de variables relacionadas con la tenencia de perros (ausencia de atención veterinaria y desparasitación no adecuada), con la presencia de MFC con parásitos y la prueba de coproantígeno positiva. Las familias con IVP medio y alto de la CP evidenciaron una fuerte asociación con la presencia de MFC con parásitos y también con alta riqueza parasitaria, mientras que las familias de la CU con IVP bajo evidenciaron MFC sin parásitos. Estos resultados revelaron un escenario vulnerable para la permanencia y la transmisión de parásitos zoonóticos caninos en la mayoría de las familias de la CP, destacando el valor de las características socio ambientales como predictores de parasitosis.

Abstract Canine zoonotic parasites have been recognized as a significant public health problem especially in developing countries with vulnerable socio-environmental conditions. In the context of “One Health” the aim of this work was to assess the animal domain by the evaluation of canine zoonotic enteroparasites associated to a Parasite Vulnerability Index (PVI) in peripheral (PC) and urban communities (UC) from Mar del Plata city. The PVI was elaborated in a previous work as a vulnerability indicator of parasite diseases, in relation to socio-environmental conditions surveyed in the communities about the next dimensions: house, sanitation, hygiene, education and work. A coproparasitological study was performed to establish the presence and the richness of canine parasites in the environment in both communities. The PC evidenced homes with a higher mean number of canine fecal samples (CFS) with parasites than the UC, showing also the highest parasite specific richness with helminths and protozoan. Frequencies of Ancylostomids, Capillariids and Toxocara canis were higher in the PC, but Trichuris vulpis frequencies and the positive coproantigen test to detect Echinococcus granulosus, were similar between communities. The PC evidenced association to variables related to dogs’ ownership (absence of veterinary attention and not adequate deworming), with the presence of CFS with parasites and the positive coproantigen test. Families with medium and high PVI from the PC evidenced a strong association with the presence of CFS with parasites and also with high parasitic richness, while families from the UC with low PVI evidenced CFS without parasites. These results revealed a vulnerable scenario for the permanence and the transmission of canine zoonotic parasites in most families from the PC, highlighting the value of the socio-environmental features as predictors of parasitoses.

Abstract Enteroparasites are an important public health problem and the treatment seeks to cure and reduce transmission. The aim of this study was to evaluate the therapeutic efficacy of anthelmintic treatment in individuals living in a rural community area in Camamu, Bahia, Brazil. The parasitological diagnosis was performed by spontaneous sedimentation, Baermann-Moraes and Agar Plate Culture methods. A total of 212 individuals were evaluated. The most frequent helminth was Trichuris trichiura, 24.5% (52/212), followed by Ascaris lumbricoides, 21.2% (45/212), hookworms, 16.5% (35/212), and S. stercoralis, 4.7% (10/212). In the anthelmintic treatment follow up, T. trichiura infection presented the lowest parasitological cure rate, only 60.6% (20/33). Hookworm, Ascaris lumbricoides and Strongyloides stercoralis infections demonstrated cure rates of 70.5 (12/17), 78.1 (25/32) and 100% (5/5), respectively. Individuals who remained infected underwent a new drug therapy. The second parasitological cure rate for T. trichiura was 38.5% (5/13), and 66.7% (2/3) and 75% (3/4) for hookworms and Ascaris lumbricoides, respectively. Trichuris trichiura infection presented the lowest parasitological cure rate at this second evaluation. This reinforces the need to perform a follow-up of all treated individuals. The possibility of drug resistance denotes the necessity for studies to clarify the mechanisms and to evaluate new therapeutic approaches. transmission Camamu Bahia Brazil sedimentation BaermannMoraes Baermann Moraes methods 21 evaluated 245 24 5 24.5 52/212, 52212 52/212 , 52 (52/212) 2 21.2 45/212, 45212 45/212 45 (45/212) 165 16 16.5 35/212, 35212 35/212 35 (35/212) S 47 4 7 4.7 10/212. 10212 10/212 . 10 (10/212) up T 606 60 6 60.6 20/33. 2033 20/33 20 33 (20/33) Hookworm 705 70 70. 12/17, 1217 12/17 12 17 (12/17) 781 78 1 78. 25/32 2532 25 32 (25/32 100 5/5, 55 5/5 (5/5) respectively therapy 385 38 38.5 5/13, 513 5/13 13 (5/13) 667 66 66.7 2/3 23 3 (2/3 75 3/4 34 (3/4 evaluation followup approaches 24. 5221 52/21 (52/212 21. 4521 45/21 (45/212 16. 3521 35/21 (35/212 4. 1021 10/21 (10/212 60. 203 20/3 (20/33 121 12/1 (12/17 25/3 253 (25/3 5/ (5/5 38. 51 5/1 (5/13 66. 2/ (2/ 3/ (3/ 522 52/2 (52/21 452 45/2 (45/21 352 35/2 (35/21 102 10/2 (10/21 20/ (20/3 12/ (12/1 25/ (25/ (5/ (5/1 (2 (3 52/ (52/2 45/ (45/2 35/ (35/2 10/ (10/2 (20/ (12/ (25 (5 ( (52/ (45/ (35/ (10/ (20 (12 (52 (45 (35 (10 (1 (4

Resumo Hortaliças contaminadas com resíduos fecais são importantes vias de transmissão de parasitas intestinais ao homem. O objetivo deste estudo foi avaliar a presença de formas parasitárias de protozoários e helmintos em alface (Lactuca sativa) e couve (Brassica oleracea) vendidas em feiras e supermercados na cidade de Aparecida de Goiânia, Goiás, Brasil. Um total de 30 amostras de cada vegetal (15 amostras de supermercados e 15 de feiras livres) foi analisado. Todas as amostras foram processadas pelos métodos de sedimentação espontânea e centrífugo-flutuação. Em 45% das amostras foram identificadas formas imaturas de parasitas intestinais sendo 66,7% ovos de helmintos e 33,3% de cistos ou oocistos de protozoários. Significativamente, mais amostras de alface estavam contaminadas com ovos, cistos ou oocistos de pelo menos um parasita do que as amostras de couve (U = 216; Z = -3,45; P <0,001). As formas parasitárias foram identificadas morfologicamente ao nível de família com ovos de Ancylostomatidae, Strongyloididae, Ascarididae e Taeniidae ou oocistos de Eimeriidae, dos gêneros Cystoisospora sp. e Toxocara sp., e ao nível de espécies com Cystoisospora canis, Dipylidium caninum e Hymenolepis nana. A presença desses agentes infecciosos em alface e couve, provenientes tanto de feiras quanto de supermercados, ressalta o alto risco de veiculação de parasitas pela ingestão de hortaliças cruas comercializadas em Aparecida de Goiânia.

Abstract Foliar vegetables contaminated with fecal residues are an important route of transmission of intestinal parasites to humans. The aim of this study was to evaluate the presence of parasitic forms of protozoa and helminths on lettuces (Lactuca sativa) and collard greens (Brassica oleracea) sold in street- and supermarkets in the city of Aparecida de Goiânia, Goiás, Brazil. A total of 30 samples of each vegetable (15 samples from each supermarkets and street markets) was analyzed. All samples were processed by spontaneous sedimentation method and centrifugal flotation. In 45% of the samples, immature forms of intestinal parasites were identified with 66.7% helminths eggs and 33.3% protozoan cysts or oocysts. Significantly more lettuce samples were contaminated with eggs, cysts or oocyst of at least one parasite than collard green samples (U=216; Z=-3.45; P <0.001). The parasitic forms were identified morphologically up to the family level with eggs of Ancylostomatidae, Strongyloididae, Ascarididae and Taeniidae, or oocysts of Eimeriidae, to the genus with Cystoisospora sp. and Toxocara sp., and to the species level with Cystoisospora canis, Dipylidium caninum and Hymenolepis nana. The presence of these infective agents in lettuce and collard green from both street- and supermarkets highlights the high risk of spreading parasites by eating raw vegetables sold in Aparecida de Goiânia.

ABSTRACT Introduction: Blastocystis spp. are among the most common intestinal parasites affecting humans. Their pathogenicity continues to be a topic of debate. Objective: Determine the prevalence of Blastocystis spp. and their association to other intestinal parasites in preschool children. Methods: Analysis was performed of 90 stool samples from preschool children. The coproparasitological techniques used were direct examination with 0.85% saline solution and lugol. The chi-square test was used to show the independence between the variables parasitic infection, sex and age. Fager's affinity index was determined to establish associations between the main enteroparasites identified. Results: The study found 68 children infected with parasites, for a 75.6% prevalence. There were no significant differences with respect to age and sex (p> 0.05). The most common parasites were Blastocystis spp. (45.6%), Endolimax nana (30.9%) and Entamoeba coli (26.5%). The only helminth found was Enterobius vermicularis (1.5%). 42.6% of the children were infected with one parasite and 57.4% with two. The affinity index (IAB) and t tests performed revealed a significant association between Blastocystis spp. and the protozoa E. nana and E. coli. Conclusions: High prevalence was determined of Blastocystis spp. and intestinal protozoan species, all of which were indicative of fecal contamination. Environmental and sociocultural factors are likely to facilitate their transmission. This topic should continue to be addressed and examined due to the relevance of this parasite in current scientific debate, both nationally and internationally.

RESUMEN Introducción: Blastocystis spp. es uno de los parásitos intestinales más comunes en el ser humano y su patogenicidad sigue siendo controvertida. Objetivo: Determinar la prevalencia de Blastocystis spp. y su asociación con otros parásitos intestinales a niños en edad preescolar. Métodos: Se procesaron 90 muestras fecales provenientes de niños en edad preescolar. Las técnicas copro-parasitológicas usadas fueron: examen directo con solución salina (0,85 %) y lugol. Se usó la prueba chi cuadrado para demostrar la independencia entre las variables: parasitosis, sexo y edad. Se determinó el índice de afinidad de Fager para establecer las asociaciones entre los principales enteroparásitos identificados. Resultados: Se encontraron 68 niños parasitados, con 75,6 % de prevalencia. No hubo diferencias significativas con respecto a la edad y el sexo (p> 0,05). Los parásitos más comunes fueron: Blastocystis spp. (45,6 %), Endolimax nana (30,9 %), Entamoeba coli (26,5 %), y como único helminto, Enterobius vermicularis (1,5 %). El 42,6 % estuvo monoparasitado y 57,4 % biparasitados. Los índices de afinidad (IAB) y pruebas de “t” efectuadas, señalaron asociación significativa entre Blastocystis spp. y los protozoarios E. nana y E. coli. Conclusiones: Se determina una elevada prevalencia de Blastocystis spp. y especies comensales intestinales, todas indicadoras de contaminación fecal, en que probablemente factores ambientales y socioculturales promueven su transmisión. Este tema debe continuarse y profundizarse por la connotación que ha adquirido este parásito en los debates científicos actuales, tanto en el ámbito nacional como internacional.

The high frequency of intestinal parasites is favored by environmental and socio-cultural conditions of indigenous populations, and is still a neglected public health problem. Mass administration of broad-spectrum drugs aims to reduce the prevalence and intensity of the infections. The prevalence of intestinal parasites in school-children in an indigenous Guarani village was evaluated before and after the mass treatment of the population with albendazole. In the first phase of collection of stool samples, 81.4% of them were positive for enteroparasites and in the second phase, after two doses of antiparasitic medication, 87.5% were positive. Although the prevalence of infections by some helminths has reduced after treatment, many parasites remained frequent in the studied population, showing a change in the epidemiological profile in the distribution of these diseases in the population. The prevalence of intestinal parasites in indigenous schoolchildren proved to be high even after mass treatment with albendazole.

A alta frequência de parasitoses intestinais é favorecida por condições ambientais e socioculturais das populações indígenas, sendo ainda um problema de saúde pública negligenciado. A administração em massa de medicamentos de amplo espectro busca reduzir o número e a intensidade das infecções. Avaliou-se a prevalência de parasitoses intestinais em escolares de uma aldeia indígena Guarani, antes e após o tratamento em massa da população com o albendazol. Na primeira fase de coleta das amostras de fezes, 81,4% delas apresentaram-se positivas para enteroparasitos e na segunda fase, após duas doses de antiparasitário, 87,5% apresentaram-se positivas. Embora a prevalência de infecções por alguns helmintos tenha reduzido após o tratamento, muitos parasitos continuaram frequentes na população estudada, mostrando uma mudança no perfil epidemiológico na distribuição dessas doenças na população. A prevalência de enteroparasitoses nos escolares indígenas mostrou-se elevada mesmo após o tratamento em massa com albendazol.

Resumen Introducción. Los enteroparásitos pueden generar problemas en animales bajo cuidado humano en zoológicos y centros de acogida. Los animales silvestres presentan bajas cargas parasitarias, pero estas pueden ser mayores y llevar a manifestaciones clínicas cuando se trata de animales resguardados en recintos, lo que aumenta los gastos en tratamientos y cuidados médicos. Por otro lado, algunos enteroparásitos pueden causar infecciones zoonóticas en los cuidadores, los visitantes y otros animales del zoológico, así como afectar los programas de recuperación de especies amenazadas de extinción. Objetivos. Determinar la presencia y prevalencia de enteroparásitos con potencial de transmisión zoonótica en primates de las familias Cebidae y Callitrichidae del Zoológico de Cali, entre septiembre y noviembre de 2017. Materiales y métodos. Se hizo un estudio transversal prospectivo, para lo cual se recolectaron muestras seriadas de 50 individuos pertenecientes a siete especies de dos familias de primates y se analizaron mediante examen coprológico, flotación y coloración Kinyoun, entre septiembre y noviembre de 2017. Resultados. Según su prevalencia, los géneros de parásitos hallados en las siete especies de primates evaluadas, fueron Blastocystis spp., Trichomonas spp., Giardia spp., Entamoeba spp., Strongyloides spp., Cyclospora sp. y Trichuris sp. Conclusiones. Por lo menos, seis de los géneros de parásitos identificados tienen implicaciones zoonóticas, lo cual hace necesario establecer las posibles vías de infección de los primates del Zoológico de Cali e implementar protocolos de manejo que reduzcan el riesgo de transmisión a los humanos y a otros animales de la colección. Además, se presenta la información relevante sobre el potencial zoonótico de los enteroparásitos hallados.

Abstract Introduction: Enteroparasites can cause problems in animals kept under human care in zoos and shelters. Wild animals have low parasitic loads but when sheltered in closed places they can be higher and lead to clinical manifestations, which increases the cost of medical treatments and care. On the other hand, some enteroparasites can represent a potential risk of zoonotic infection for their animal keepers, visitors, and other zoo animals. In addition, they could affect recovery programs for endangered species. Objectives: To establish the presence and prevalence of potentially zoonotic enteroparasites in primates of the Cebidae and Callitrichidae families at the Zoológico de Cali from September to November, 2017. Materials and methods: We conducted a prospective cross-sectional study. Serial samples from 50 individuals belonging to seven species and two primate families were analyzed by ova and parasite test, flotation, and Kinyoun stain between September and November, 2017. Results: In order of frequency, the parasite genera found in the seven primate species evaluated were Blastocystis spp.,Trichomonas spp., Giardia spp., Entamoeba spp., Strongyloides spp., Cyclospora sp., and Trichuris sp. Conclusions: At least six of the parasite genera found have potential zoonotic implications. It is necessary to establish what are the infection sources at the Zoológico de Cali and implement management protocols to reduce the risk of transmission to both humans and other animals in the collection. Additionally, we offer relevant information on the zoonotic potential of each of the enteroparasites found.

ABSTRACT Objective To monitor the frequency of enteroparasites in lettuce (Lactuca sativa) and parsley (Petrosolium sativum) samples, marketed in commercial buildings in the municipality of Conceição da Barra, Espírito Santo, Brazil. Materials and Methods Between August 2015 and July 2016, four different popular commercial buildings (fairs, markets and supermarkets) were selected as the largest vegetable suppliers in the municipality of Conceição da Barra, Espírito Santo. A total of 40 samples were analyzed using the modified spontaneous sedimentation method at the Laboratory of Parasitology and Hematology of Centro Universitário Norte do Espirito Santo, Universidade Federal do Espírito Santo. Results The most frequent organism in the study population were the commensal Entamoeba coli and the parasites E. histolytica/E.dispar, hookworm, Balantidium coli, Ascaris sp., Toxocara sp., Taenia sp. and Dipylidium sp. Conclusions The results indicate a high degree of contamination of the vegetables, probably related to the precariousness of production, transportation and handling, making it necessary to adopt measures that guarantee the best quality of food and, consequently, better population health.

RESUMEN Objetivo Monitorear la frecuencia de enteroparásitos en muestras de lechuga (Lactuca sativa) y perejil (Petrosolium sativum), comercializadas en edificios comerciales en el municipio de Conceição da Barra, Espírito Santo, Brasil. Materiales y Métodos Entre agosto de 2015 y julio de 2016, cuatro diferentes edificios comerciales populares (ferias, mercados y supermercados) fueron seleccionados como los proveedores de vegetales más grandes en el municipio de Conceição da Barra, Espírito Santo. Se analizaron un total de 40 muestras utilizando el método modificado de sedimentación espontánea en el Laboratorio de Parasitología y Hematología del Centro Universitario Norte del Espírito Santo, Universidade Federal del Espírito Santo. Resultados Los organismos más frecuentes en la población de estudio fue el comensal Entamoeba coli y los parásitos E. histolytica/E. dispar, ancilostomídeo, Balantidium coli, Ascaris sp., Toxocara sp., Taenia sp. y Dipylidium sp. Conclusiones Los resultados indican un alto grado de contaminación de las hortalizas, probablemente relacionado con la precariedad de la producción, el transporte y la manipulación, por lo que es necesario adoptar medidas que garanticen la mejor calidad de los alimentos y, en consecuencia, una mejor salud de la población.

RESUMEN Objetivo Monitorear la frecuencia de enteroparásitos en muestras de lechuga (Lactuca sativa) y perejil (Petrosolium sativum), comercializadas en edificios comerciales en el municipio de Conceição da Barra, Espírito Santo, Brasil. Materiales y Métodos Entre agosto de 2015 y julio de 2016, cuatro diferentes edificios comerciales populares (ferias, mercados y supermercados) fueron seleccionados como los proveedores de vegetales más grandes en el municipio de Conceição da Barra, Espírito Santo. Se analizaron un total de 40 muestras utilizando el método modificado de sedimentación espontánea en el Laboratorio de Parasitología y Hematología del Centro Universitario Norte del Espírito Santo, Universidade Federal del Espírito Santo. Resultados Los organismos más frecuentes en la población de estudio fue el comensal Entamoeba coli y los parásitos E. histolytica/E. dispar, ancilostomídeo, Balantidium coli, Ascaris sp., Toxocara sp., Taenia sp. y Dipylidium sp. Conclusiones Los resultados indican un alto grado de contaminación de las hortalizas, probablemente relacionado con la precariedad de la producción, el transporte y la manipulación, por lo que es necesario adoptar medidas que garanticen la mejor calidad de los alimentos y, en consecuencia, una mejor salud de la población.

ABSTRACT Objective To monitor the frequency of enteroparasites in lettuce (Lactuca sativa) and parsley (Petrosolium sativum) samples, marketed in commercial buildings in the municipality of Conceição da Barra, Espírito Santo, Brazil. Materials and Methods Between August 2015 and July 2016, four different popular commercial buildings (fairs, markets and supermarkets) were selected as the largest vegetable suppliers in the municipality of Conceição da Barra, Espírito Santo. A total of 40 samples were analyzed using the modified spontaneous sedimentation method at the Laboratory of Parasitology and Hematology of Centro Universitário Norte do Espirito Santo, Universidade Federal do Espírito Santo. Results The most frequent organism in the study population were the commensal Entamoeba coli and the parasites E. histolytica/E.dispar, hookworm, Balantidium coli, Ascaris sp., Toxocara sp., Taenia sp. and Dipylidium sp. Conclusions The results indicate a high degree of contamination of the vegetables, probably related to the precariousness of production, transportation and handling, making it necessary to adopt measures that guarantee the best quality of food and, consequently, better population health.

ABSTRACT Objective To monitor the frequency of enteroparasites in lettuce (Lactuca sativa) and parsley (Petrosolium sativum) samples, marketed in commercial buildings in the municipality of Jaguaré, Espírito Santo, Brazil. Materials and Methods Between August 2016 and July 2017, five different popular commercial buildings (markets, supermarkets and fairs) in the municipality of Jaguaré, considered the main local vegetable suppliers were selected. Throughout the research, 30 vegetable samples were randomly selected for parasitological analysis in 3 replicates for each commercial building under study. The obtained samples were analyzed by the modified spontaneous sedimentation method at the Laboratory of Parasitology and Hematology of Universidade Federal do Espírito Santo. Results Of the 30 samples analyzed, 83.3% were positive for parasitic contamination and the most common parasites were Hookworm, Balantidium coli, Isospora belli, Ascaris sp. and Entamoeba sp. Conclusions The results indicate a high degree of contamination of the vegetables, probably related to the precariousness of production, transportation and handling, making it necessary to adopt measures that ensure the best quality of food and, consequently, better population health.

RESUMEN Objetivo Monitorear la frecuencia de enteroparásitos en muestras de lechuga (Lactuca sativa) y perejil (Petrosolium sativum), comercializadas en edificios comerciales en el municipio de Jaguaré, Espírito Santo, Brasil. Materiales y Métodos Entre agosto de 2016 y julio de 2017, se seleccionaron cinco diferentes edificios comerciales populares (mercados, supermercados y ferias) en el municipio de Jaguaré, considerados los principales proveedores locales de vegetales. A lo largo de la investigación, se seleccionaron al azar 30 muestras de vegetales para el análisis parasitológico en 3 repeticiones para cada edificio comercial en estudio. Las muestras obtenidas fueron analizadas por el método modificado de sedimentación espontánea en el Laboratorio de Parasitología y Hematología de la Universidade Federal del Espírito Santo. Resultados De las 30 muestras analizadas 83.3% fueron positivas para la contaminación parasitaria y los parásitos más comunes fueron Anquilostoma, Balantidium coli, Isospora belli, Ascaris sp. y Entamoeba sp. Conclusiones Los resultados indican un alto grado de contaminación de las hortalizas, probablemente relacionado con la precariedad de la producción, el transporte y la manipulación, por lo que es necesario adoptar medidas que aseguren la mejor calidad de los alimentos y, en consecuencia, una mejor salud de la población.

ABSTRACT Objective To monitor the frequency of enteroparasites in lettuce (Lactuca sativa) and parsley (Petrosolium sativum) samples, marketed in commercial buildings in the municipality of Jaguaré, Espírito Santo, Brazil. Materials and Methods Between August 2016 and July 2017, five different popular commercial buildings (markets, supermarkets and fairs) in the municipality of Jaguaré, considered the main local vegetable suppliers were selected. Throughout the research, 30 vegetable samples were randomly selected for parasitological analysis in 3 replicates for each commercial building under study. The obtained samples were analyzed by the modified spontaneous sedimentation method at the Laboratory of Parasitology and Hematology of Universidade Federal do Espírito Santo. Results Of the 30 samples analyzed, 83.3% were positive for parasitic contamination and the most common parasites were Hookworm, Balantidium coli, Isospora belli, Ascaris sp. and Entamoeba sp. Conclusions The results indicate a high degree of contamination of the vegetables, probably related to the precariousness of production, transportation and handling, making it necessary to adopt measures that ensure the best quality of food and, consequently, better population health.

RESUMEN Objetivo Monitorear la frecuencia de enteroparásitos en muestras de lechuga (Lactuca sativa) y perejil (Petrosolium sativum), comercializadas en edificios comerciales en el municipio de Jaguaré, Espírito Santo, Brasil. Materiales y Métodos Entre agosto de 2016 y julio de 2017, se seleccionaron cinco diferentes edificios comerciales populares (mercados, supermercados y ferias) en el municipio de Jaguaré, considerados los principales proveedores locales de vegetales. A lo largo de la investigación, se seleccionaron al azar 30 muestras de vegetales para el análisis parasitológico en 3 repeticiones para cada edificio comercial en estudio. Las muestras obtenidas fueron analizadas por el método modificado de sedimentación espontánea en el Laboratorio de Parasitología y Hematología de la Universidade Federal del Espírito Santo. Resultados De las 30 muestras analizadas 83.3% fueron positivas para la contaminación parasitaria y los parásitos más comunes fueron Anquilostoma, Balantidium coli, Isospora belli, Ascaris sp. y Entamoeba sp. Conclusiones Los resultados indican un alto grado de contaminación de las hortalizas, probablemente relacionado con la precariedad de la producción, el transporte y la manipulación, por lo que es necesario adoptar medidas que aseguren la mejor calidad de los alimentos y, en consecuencia, una mejor salud de la población.

Resumo Os resultados obtidos de estudos parasitológicos em amostras fecais e esfregaços anais enviados ao Laboratório de Parasitologia e Gastroenterologia do Hospital de Pediatría “Prof. Dr. Juan P. Garrahan” da Cidade de Buenos Aires, Argentina, foram avaliados retrospectivamente. O objetivo do estudo foi avaliar a prevalência dos parasitas intestinais observados nas amostras enviadas a este hospital e sua distribuição por faixas etárias. Durante o período compreendido entre maio de 2018 a abril de 2019 foram analisadas 4713 amostras, correspondentes a 3311 pacientes, o que deu um resultado total de 29% de positivos (1371/4713). 30% dos exames coproparasitológicos foram positivos (1193/4025) e foram encontrados ovos de Enterobius vermicularis em 26% (178/688) dos esfregaços anais. Nas amostras seriadas fecais foram obtidos 37% de resultados positivos, enquanto que em amostras únicas, 21%. Os enteroparasitas mais prevalentes na totalidade das amostras fecais foram Blastocystis spp. em 14% (579/4025), Giardia duodenalis 11% (456/4025) e Dientamoeba fragilis 7% (291/4025). Os resultados demonstraram diferenças significativas com maior prevalência de parasitas intestinais em pacientes em idade escolar da faixa etária entre 6 e 10 anos, em relação aos da pré-escola. Nas amostras fecais analisadas, evidenciou-se um predomínio de parasitismo por protozoários por sobre helmintos, pelo qual deve ser considerado o reforço do diagnóstico laboratorial dessas espécies por meio de técnicas, como colorações que permitam a sua localização e confirmação.

Abstract The results obtained of the coproparasitological studies and anal swab submitted to the laboratory of the Parasitology Section of the Hospital de Pediatría “Prof. Dr. Juan P. Garrahan” of the Buenos Aires City, Argentina, were retrospectively evaluated The aim of this study was to determine the prevalence of intestinal parasites found on the submitted samples and to evaluate their frequency according to the age of the patients. Within the period between May 2018 and April 2019, a total of 4713 samples corresponding to 3311 patients were analyzed and intestinal parasites were present in 29% (1371/4713). In 30% of coproparasitological studies parasite estructures were found (1193/4025) and 26% of anal swab samples were positive for the presence of Enterobius vermicularis eggs (178/688). In serial fecal samples, 37% positive results were obtained, while in single samples, 21%. The most prevalent enteroparasites calculated from the total fecal samples were: Blastocystis spp. with 14% (579/4025), followed by Giardia duodenalis 11% (456/4025) and Dientamoeba fragilis 7% (291/4025). The incidence of intestinal parasites was higher in the group of patients between 6 and 10 years, and the existence of significant differences between proportions of parasitized patients in preschool and school infants, was observed. In the faecal samples analyzed, a greater prevalence of parasitism caused by protozoa than by helminths was evidenced, for which reason it should be considered, reinforcing the laboratory diagnosis using techniques such as stains that allow its finding and confirmation.

Resumen Se evaluaron retrospectivamente los resultados obtenidos de estudios parasitológicos en muestras de heces y escobillados anales remitidos al Laboratorio de Parasitología y Gastroenterología del Hospital de Pediatría “Prof. Dr. Juan P. Garrahan” de la Ciudad de Buenos Aires, Argentina. El objetivo del trabajo fue conocer la prevalencia de los parásitos intestinales observados en las muestras remitidas a este hospital y su distribución en rangos etarios. En el período comprendido entre mayo de 2018 y abril de 2019 se analizó un total de 4713 muestras pertenecientes a 3311 pacientes, que arrojó un resultado total de 29% de positivos (1371/4713). De los exámenes coproparasitológicos el 30% fueron positivos (1193/4025) y se hallaron huevos de Enterobius vermicularis en el 26% (178/688) de los escobillados anales. En las muestras seriadas de heces se obtuvo un 37% de positividad mientras que en muestras únicas un 21%. En la totalidad de muestras fecales, los enteroparásitos más prevalentes fueron Blastocystis spp. en un 14% (579/4025), Giardia duodenalis 11% (456/4025) y Dientamoeba fragilis 7% (291/4025). Los resultados demostraron diferencias significativas con mayor prevalencia de parásitos intestinales en pacientes en edad escolar comprendidos entre los 6 y 10 años, en relación a los de edad preescolar. En las muestras fecales analizadas se evidenció un predominio de parasitismo de protozoos sobre helmintos, por lo que se debería considerar reforzar el diagnóstico de laboratorio de esas especies mediante técnicas, como coloraciones, que posibiliten su hallazgo y confirmación.

ABSTRACT The aim of this study was to determine the association of enteroparasitosis in school-age children in the Santa María de Chicmo district (Apurímac, Peru) with the nutritional status, characteristics of the house, and raising of backyard animals. Stool samples were taken from 493 children aged 6-16 years, of both sexes, and their homes were visited to determine their characteristics. The prevalence of enteroparasites was 82.4%, where the highest prevalence corresponded to Entamoeba coli (59.6%), followed by Ascaris lumbricoides (51.1%) and Taenia sp with 33.3%. Other parasites presented prevalence less than 12%. The raising of pigs and chickens was associated with Taenia sp and E. vermicularis, respectively. Defecating in the open field, housing on the edge of crops and on the edge of ditches were associated with E. vermicularis, H. nana and A. lumbricoides. Nutritional status was not associated with parasitosis in school children. It is concluded that the characteristics of the houses and the raising of backyard animals present characteristics that favour the presentation of intestinal parasitism in children in the Santa María de Chicmo district.

RESUMEN El objetivo del estudio fue determinar la asociación de la enteroparasitosis en niños en edad escolar del distrito de Santa María de Chicmo (Apurímac, Perú) con el estado nutricional, características de la vivienda y crianza de animales de traspatio. Se tomaron muestras coprológicas a 493 niños de 6-16 años, de ambos sexos y se visitaron sus viviendas para determinar sus características. La prevalencia de enteroparásitos fue de 82.4%, donde la mayor prevalencia correspondió a Entamoeba coli (59.6%), seguida de Ascaris lumbricoides (51.1%) y Taenia sp con 33.3%. Otros parásitos presentaron prevalencias menores a 12%. La crianza de porcinos y pollos estuvo asociada a Taenia sp y E. vermicularis, respectivamente. Defecar a campo abierto, vivienda al borde de cultivos y al borde de acequias estuvieron asociados a E. vermicularis, H. nana y A. lumbricoides. El estado nutricional no estuvo asociado a la parasitosis en los escolares. Se concluye que las características de las viviendas y la crianza de animales de traspatio presentan características que favorecen la presentación de parasitismo intestinal en los niños del distrito de Santa María de Chicmo.

Resumo Introdução As creches desempenham um importante papel social na educação e desenvolvimento inicial das crianças. O objetivo deste estudo foi investigar a frequência de infecções parasitárias intestinais, além de infecção por Toxocara spp. em crianças de uma creche na cidade do Rio Grande, Rio Grande do Sul, Brasil. Métodos: Para o estudo, foram coletadas amostras fecais de 50 crianças de três a seis anos, analisadas pelas técnicas de Ritchie, Faust, Rugai e Kinyoun, sendo aplicado um questionário epidemiológico fechado e estruturado aos pais/responsáveis das crianças. Também foi realizada uma pesquisa sorológica de anticorpos para Toxocara spp. em 41 crianças, pelo ensaio imunoenzimático, associado ao antígeno de excreção e secreção (ES), com soros pré-adsorvidos com antígeno somático de Ascaris lumbricoides. Resultados: A frequência de enteroparasitos foi de 18%, enquanto que 43.9% das crianças foram soropositivas para Toxocara spp.. Baixa renda familiar e baixo nível de alfabetização dos pais/responsáveis foram fatores comuns entre as famílias das crianças parasitadas. A maioria dos pais ou responsáveis das crianças, com diagnóstico positivo para enteroparasitos ou para Toxocara spp., relatou utilizar apenas água para a higienização de verduras e frutas. Conclusões: As altas taxas de soropositividade para Toxocara spp. indicam que as crianças foram expostas a esse parasito, sendo importante minimizar o risco de infecção. Além disso, a positividade de 18% para enteroparasitos e os dados obtidos com as entrevistas realizadas com os pais ou responsáveis demonstram a necessidade da realização de trabalhos com a população estudada que visem a divulgação sobre as parasitoses intestinais, especialmente sobre medidas profiláticas especificas.

Abstract Introduction Day care centers play an important social role in children’s early education and development. The objective of this study was to investigate the frequency of intestinal parasitic infections and infection with Toxocara spp. in children from a day care center in the city of Rio Grande, Rio Grande do Sul, Brazil. Methods: The study was conducted using fecal samples from 50 children, ages three to six years, using Ritchie, Faust, Rugai, and Kinyoun’s techniques. A closed and structured epidemiological questionnaire was used to collect data from the parents/guardians of the children. Serological studies for detection of antibodies to Toxocara spp. by immunoenzymatic assays using Toxocara excretion and secretion antigen (TES) were conducted on sera collected from 41 children. Results: The frequency of enteroparasites was 18%, with 43.9% of the children testing seropositive for Toxocara spp. Low family income and low literacy levels of parents/guardians were common factors between the families of the parasite-positive children. For the children who tested positive for Toxocara spp., most of the parents/guardians reported using only water for sanitizing raw vegetables and fruits. Conclusions: The high seropositivity rates for Toxocara spp. indicate that children were exposed to this parasite, and it is important to reduce the risk of infection. In addition, the seropositivity for enteroparasites and the interviews with the parents/guardians indicate the need to educate the studied population regarding the modes of intestinal parasite transmission and the prophylactic measures needed to prevent their dissemination.

Resumen Introducción. La falta de estudios en intercomunicadores como medios de propagación de enteroparásitos en Venezuela, motivó el presente estudio. Metodología. Se frotó el intercomunicador tres veces con un hisopo impregnado con solución salina 0,85% (SSI), resuspendiéndolo en un tubo con 10 ml de SSI, el cual se centrifugó a 3000 rpm por 10 minutos para luego observar el sedimente al microscopio. Resultados. El 65% de las muestras estuvieron contaminadas, encontrando 10 taxones de enteroparásitos, a saber: Blastocystis sp (49% del total de muestras examinadas), Endolimax nana (36%), lodamoeba butschlii (8%), Giardia lamblia (7%), Entamoeba coli (4%), Chilomastix mesnili (2%), Entamoeba hartmanni (2%), Trichuris trichiura (2%), Hymenolepis nana (2%) y Taenia sp (1%). Discusión. La diversidad de parásitos encontrados y su abundancia, se corresponden con lo reportado en poblaciones de la misma zona. Conclusión. La falta de higiene en la mayoría de los intercomunicadores facilitan el potencial de estos para transmitir los parásitos endémicos del estado Lara.

Abstract Introduction. The lack of studies in intercoms as means of propagation of enteroparasites in Venezuela, motivated the present study. Methodology. The intercom was rubbed three times with a swab impregnated with 0.85% saline solution (SSI), resuspending it in a tube with 10 ml of SSI, which was centrifuged at 3000 rpm for 10 minutes to then observe the pellet under a microscope. Results. 65% of the samples were contaminated, finding 10 taxa of enteroparasites, namely: Blastocystis sp (49% of the samples examined), Endolimax nana (36%), Iodamoeba butschlii (8%), Giardia lamblia (7%), Entamoeba coli (4%), Chilomastix mesnili (2%), Entamoeba hartmanni (2%), Trichuris trichiura (2%), Hymenolepis nana (2%) and Taenia sp (1%). Discussion. The diversity of parasites found and their abundance correspond to that reported in populations of the same area. Conclusion. The lack of hygiene of most of the intercoms facilitates the potential of these to transmit the endemic parasites of the Lara State.