Abstract Introduction: Macrobrachium amazonicum is widely distributed in South America, occurring in the Orinoco, Amazon and Paraguay river basins, being used as an important source of protein for feeding riverside populations. This prawn is host to the crustacean ectoparasites Probopyrus pandalicola, Probopyrus floridensis and Probopyrus bithynis (Bopyridae) that infest the gill cavity of the species. Objective: To report the prevalence and abundance of ectopasites (P. pandalicola, P. floridensis and P. bithynis) and the condition factor in M. amazonicum from the Amazon River, Eastern Amazon. Methods: The prawns were collected from May 2017 to April 2018, with the artisanal trap matapi in the municipalities of Mazagão (state of Amapá) and Santarém (state of Pará), Brazil. Results: 216 prawns infected with ectoparasites were captured, 133 in the Mazagão region (53 P. pandalicola, 48 P. bithynis and 32 P. floridensis) and 83 in the Santarém region (45 P. pandalicola and 38 P. bithynis). In Mazagão, the highest prevalence values were recorded in May (1.98 %) for P. floridensis, July (2.18 %) for P. bithynis, and March (1.93 %) for P. pandalicola. In the Santarém region, the highest prevalence values were recorded in February, 3.47 for P. pandalicola and 2.89 for P. bithynis. Regarding the abundance of parasites, there was no difference between the species grouped between the regions (t = 0.32, p = 0.739). Parasitized prawns from Mazagão and Santarém presented condition factors below expectations, with an average of 0.85 ± 0.55 for Santarém and 0.88 ± 0.44 for Mazagão. Conclusions: During the study it was found that P. pandalicola, P. bithynis and P. floridensis were present in M. amazonicum throughout the year. Furthermore, there was an effect of parasitism on M. amazonicum in the study areas.

Resumen Introducción: Macrobrachium amazonicum está ampliamente distribuido en América del Sur, se encuentra en las cuencas de los ríos Orinoco, Amazonas y Paraguay, se utiliza como una fuente importante de proteínas para alimentar a las poblaciones ribereñas. Este camarón es hospedero de los ectoparásitos crustáceos: Probopyrus pandalicola, Probopyrus floridensis y Probopyrus bithynis (Bopyridae), que infestan la cavidad branquial de la especie. Objetivo: Reportar la prevalencia y abundancia de ectoparásitos (P. pandalicola, P. floridensis y P. bithynis) y el factor de condición de M. amazonicum en el| río Amazonas, al este de la Amazonía. Métodos: Los camarones fueron recolectados entre mayo 2017 y abril 2018, con la ayuda de matapis (trampa artesanal) en los municipios de Mazagão (Estado de Amapá) y Santarém (Estado de Pará), Brasil. Resultados: Se capturaron un total de 216 camarones parasitados con ectoparásitos, 133 de ellos en la región de Mazagão (53 P. pandalicola, 48 P. bithynis y 32 P. floridensis) y 83 en la región de Santarém (45 P. pandalicola y 38 P. bithynis). En Mazagão, los valores más altos de prevalencia se registraron en mayo (1.98 %) para P. floridensis, julio (2.18 %) para P. bithynis y marzo (1.93 %) para P. pandalicola. En la región de Santarém, los valores más altos de prevalencia se registraron en febrero, de 3.47 para P. pandalicola y 2.89 para P. bithynis. En cuanto a la abundancia de parásitos, no hubo diferencia entre las especies agrupadas entre las regiones (t = 0.32, p = 0.739). Los camarones parasitados de Mazagão y Santarém mostraron factores de condición por debajo de lo esperado, con un promedio de 0.85 ± 0.55 para Santarém y 0.88 ± 0.44 para Mazagão. Conclusiones: Durante el estudio, se encontró que P. pandalicola, P. bithynis y P. floridensis estuvieron presentes en M. Amazonicum todo el año. Además, hubo un efecto de parasitismo sobre M. Amazonicum en las áreas de estudio.

ABSTRACT The ecology, distribution, and taxonomy of bopyrid isopod species are often omitted from studies not systematically addressed to crustaceans. From a wider study of zooplankton in coastal and reef waters of the Mexican Caribbean Sea, two bopyrid species were collected, Probopyrinella latreuticola, attached to the branchial chamber of Latreutes fucorum andProbopyrus pandalicola parasitizing the branchial chamber of Palaemon northropi. For both parasite-host associations, these records represent a new locality and the southernmost record in the Mexican Caribbean. A map and a list of the bopyrid species belonging to Bopyridae recorded in the Mexican Caribbean are provided.

RESUMEN El objetivo de este estudio fue determinar la prevalencia de Ehrlichia spp, Anaplasma spp, Dirofilaria immitis y la presencia de Borrelia burgdorferi en caninos de la ciudad de Guayaquil. Se tomaron muestras de sangre a 220 canes y se analizaron mediante la técnica de ELISA (Snap 4Dx Plus, Idexx®). En 120 de estos canes se determinó la presencia de ectoparásitos y sus muestras sanguíneas fueron analizadas además mediante frotis para contrastar los resultados con los obtenidos por ELISA. Los resultados se analizaron mediante Chi cuadrado y el test exacto de Fisher para determinar la asociación de riesgo y la influencia de los factores de riesgo (edad, sexo y zona) basado en Odds Ratio. Se hizo la prueba de Kappa para determinar la concordancia entre las pruebas utilizadas. La prevalencia de Erhlichia spp fue de 75.4% (166/220), Anaplasma spp de 22.8% (49/220), D. immitis de 2.3% (5/220) y nula para B. burgdorferi. Se encontró coinfección de Erhlichia spp y Anaplasma spp (20.9%) y de Erhlichia spp y D. inmitis (2.3%). No se encontró asociación (p>0.05) entre estos patógenos con respecto a la edad, sexo o la zona donde habitan los animales. El nivel de concordancia del índice Kappa entre la técnica de ELISA y el frotis sanguíneo fue aceptable (Erhlichia spp = 0.79; Anaplasma spp = 0.91), pero una mayor sensibilidad en el método de ELISA.

ABSTRACT The aim of this study was to determine the prevalence of Ehrlichia spp, Anaplasma spp, Dirofilaria immitis and the presence of Borrelia burgdorferi in canines from the city of Guayaquil. Blood samples were taken from 220 dogs and analysed using the ELISA technique (Snap 4Dx Plus, Idexx®). In 120 of these dogs the presence of ectoparasites was determined and their blood samples were also analysed by smears to compare the results with those obtained by ELISA. The results were analysed using Chisquare and Fisher’s exact test to determine the risk association and the influence of risk factors (age, sex, and area) based on the Odds Ratio. The Kappa test was performed to determine the agreement between the tests used. The prevalence of Erhlichia spp was 75.4% (166/220), Anaplasma spp 22.8% (49/220), D. immitis 2.3% (5/220) and null for B. burgdorferi. Coinfection of Erhlichia spp and Anaplasma spp (20.9%) and Erhlichia spp and D. immitis (2.3%) was found. No association (p>0.05) was found between these pathogens with respect to age, sex or the area where the animals live. The level of concordance of the Kappa index between the ELISA technique and the blood smear was acceptable (Erhlichia spp = 0.79; Anaplasma spp = 0.91), but a higher sensitivity in the ELISA method.

Abstract 19. This study reports an episode of fish mortality occurred in the Garupá and Pindapoy Grande streams, near the confluence with the Paraná River (Garupá, Argentina). Dead and moribund fish of various species were observed floating on the water surface. Environmental parameters measured were temperature 5 °C, water temperature 15.1 (±0.6) °C, dissolved oxygen 8.7 (±1.2) ppm, pH 7.5 (±0.2), electrical conductivity 67.6 (± 8.8) mS cm-1 and mean depth of 1.7 (±1.0) m. Macroscopic examination of moribund fish revealed corneal opacity, whitish tegument, increased gill mucus, and whitish areas on gill filaments. Microscopic analysis of gills revealed severe generalized epithelial hyperplasia, lamellar fusion, congestion, and inflammatory infiltrate, particularly lymphocytes and eosinophilic granular cells. In addition, microscopic examination allowed the identification of the following ectoparasites found in gills and tegument: Chilodonella, Ichthyophthirius, Dactylogyridae and Trichodinidae, representing 98%, 1.2%, 0.6% and 0.2%, respectively. This study constitutes a novel record of wild fish mortality associated with extreme environmental conditions and parasitosis. Furthermore, our findings are significant as there are no prior reports in Argentina of epizootic cases linked to Chilodonella sp. infection in wild or farmed fish populations, being the first report of chilodonelliasis for this region.

Resumen 23. Este estudio reporta un episodio de mortandad de peces ocurrido en los arroyos Garupá y Pindapoy Grande, cerca de la confluencia con el río Paraná (Garupá, Argentina). En este tramo se encontraron flotando en la superficie del agua, peces moribundos y muertos de diferentes especies. Los parámetros medidos fueron: temperatura ambiental 5 °C, temperatura del agua 15,1 (±0,6) °C, oxígeno disuelto 8,7 (±1,2) ppm, 24. pH 7,5 (±0,2), conductividad 67,6 (± 8,8) mS cm-1 y profundidad media de 1,7 (±1,0) m. En el examen macroscópico de peces moribundos se observó opacidad corneal, tegumento blanquecino, aumento de moco branquial y áreas blanquecinas en los filamentos branquiales. El análisis microscópico de las branquias reveló hiperplasia epitelial generalizada grave, fusión laminar, congestión e infiltrado inflamatorio, particularmente linfocitos y células granulares eosinófilas. Además, el examen microscópico permitió identificar los siguientes ectoparásitos encontrados en piel y branquias: Chilodonella, Ichthyophthirius, Dactylogyridae y Trichodinidae, que representó el 98%; 1,2%; 0,6% y 0,2%, respectivamente. El presente estudio es un nuevo registro de mortandad de peces silvestres relacionada con condiciones ambientales extremas y parasitosis. Además, nuestro hallazgo aporta información valiosa, ya que en Argentina no existen registros de casos epizoóticos asociados a infección por Chilodonella sp. en poblaciones de peces silvestres o de criaderos, siendo el primer reporte de quilodonelliasis para esta región.

RESUMO: Ratos e camundongos são os modelos experimentais mais utilizados em pesquisa. Globalmente, as instalações de animais estão sujeitas a surtos de parasitas. No Brasil, o perfil parasitológico é utilizado para refletir a condição sanitária dos animais de laboratório e deve ser monitorado frequentemente. O presente estudo teve como objetivo desenvolver uma revisão integrativa de estudos científicos sobre o perfil parasitológico de ratos e camundongos em biotérios no Brasil. A revisão identifica as espécies parasitárias mais prevalentes relatadas em biotérios de diferentes regiões geográficas do país, bem como os fatores que contribuem para a perpetuação desses parasitas, e propõe medidas para ajudar a prevenir tais infecções. Com base na questão norteadora “quais endoparasitas e ectoparasitas já foram identificados em colônias de ratos e camundongos em biotérios no Brasil?” e considerando os critérios de inclusão e exclusão, foram selecionados 28 estudos publicados entre 1974 e 2021 em quatro bases científicas. Esses estudos abrangeram instalações em 12 estados brasileiros, descrevendo 16 gêneros de parasitas em camundongos e 18 em ratos. A maioria das instalações dos estudos selecionados era do tipo convencional e com poucas barreiras sanitárias, e essas condições podem favorecer a recorrência de parasitas. Portanto, esforços são necessários para que as instituições tenham biotérios de acordo com a legislação e pratiquem metodologias otimizadas. As medidas propostas neste artigo podem contribuir para mudar o panorama das parasitoses nos biotérios nacionais, visando salvaguardar a qualidade dos dados científicos e o bem-estar animal. RESUMO pesquisa Globalmente Brasil frequentemente país infecções quais Brasil? exclusão 2 197 202 científicas 1 brasileiros sanitárias Portanto otimizadas nacionais bemestar estar animal 19 20

ABSTRACT: Rats and mice are the most used experimental models in research. Globally, animal houses are subjected to parasite outbreaks. In Brazil, the parasitological profile is used to reflect the sanitary condition of laboratory animals and must be monitored frequently. The present study developed an integrative review of scientific studies on the parasitological profile of rats and mice in animal facilities in Brazil. It identified the most prevalent parasite species reported in animal facilities from different geographic regions of the country, as well as factors contributing to the perpetuation of these parasites, and proposed measures to help prevent such infections. Based on the guiding question “which endoparasites and ectoparasites have already been identified in rat and mouse colonies in animal facilities in Brazil?” and considering inclusion and exclusion criteria, 28 studies published between 1974 and 2021 were selected in four scientific bases. These studies covered facilities in 12 Brazilian States, describing 16 genera of parasites in mice and 18 in rats. Most of the facilities in the selected studies were of the conventional type and with few sanitary barriers, and these conditions may favor the recurrence of parasites. Efforts are, therefore, necessary for institutions to have animal facilities in accordance with the legislation and practice optimal methodologies. The measures proposed in this article can contribute to change the panorama of parasites in the national animal facilities, aiming to safeguard the quality of scientific data and animal welfare. ABSTRACT research Globally outbreaks Brazil frequently country infections which Brazil? criteria 2 197 202 bases 1 States barriers therefore methodologies welfare 19 20

RESUMO Amostras de sangue e fezes de perdiz chukar (Alectoris chukar), faisão-albino (Phasianus colchicus), faisão-prateado (Lophura nycthemera), periquito-de-rosa (Psittacula krameri) e perus (Meleagris gallopavo) foram analisadas para verificar a prevalência de parasitas. Para registrar os parasitas, essas cinco espécies de aves foram colocadas em gaiolas separadas no Centro de Conservação e Pesquisa de Aves, Departamento de Vida Selvagem e Ecologia, Universidade de Veterinária e Ciências Animais, Lahore, Paquistão. Cem amostras fecais e 100 amostras de sangue para cada espécie de ave foram inspecionadas para analisar os parasitas internos. Durante o presente estudo, foram examinadas 17 espécies de endoparasitas, 14 de amostras fecais e 3 de sangue. Foram estudadas duas espécies de ectoparasitas, ou seja, o ácaro Dermanyssus gallinae 42% e o carrapato aviário Args persicus 41%. Os parasitas sanguíneos incluíram Plasmodium juxtanucleare 50%, Leucoctoyzoon simond com prevalência parasitária de 40% e Aegyptinella pullorum com prevalência parasitária de 40%. As espécies parasitas registradas em amostras fecais incluíram 6 espécies de nematoides viz. Allodpa suctoria 2%, Syngamus traqueia com prevalência parasitária de 60%, Capillaria annulata 37,5%, Ascardia galli 24%, Capillaria anatis 40% e Heterakis gallinarum 28,3%. Da mesma forma, duas espécies de trematódeos viz. Prosthogonimus ovatus com prevalência parasitária de 50% e Prosthogonimus macrorchis 21% também foram documentados em amostras fecais de aves. Espécies de cestoide único Raillietina echinobothrida com prevalência parasitária de 72% e 3 espécies de protozoários, isto é, Eimeria maxima com prevalência parasitária de 21%, Giardia lamblia 41% e Histomonas meleagridis 18% foram documentadas durante a análise corpológica. Em nossa recomendação, o saneamento adequado, medicação e vacinação de invólucros de pássaros são sugeridos para evitar parasitas.

Abstract Blood and fecal samples of chukar partridge (Alectoris chukar), albino pheasant (Phasianus colchicus), silver pheasant (Lophura nycthemera), rose-ringed parakeet (Psittacula krameri) and turkeys (Meleagris gallopavo) were analyzed to check parasitic prevalence. To record parasites these five avian species were placed kept in separate cages at Avian Conservation and Research Center, Department of Wildlife an Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan. 100 fecal and 100 blood samples for each bird species were inspected to analyze internal parasites. During present study, 17 species of endoparasites 14 from fecal samples and three from blood were examined. Two species of ectoparasites i.e. mite Dermanyssus gallinae 42% and fowl ticks Args persicus 41%were studied. Blood parasites included Plasmodium juxtanucleare 50%, Leucoctoyzoon simond having parasitic prevalence 40%, and Aegyptinella pullorum having parasitic prevalence of 40%. Parasitic species recorded from fecal samples included 6 species of nematodes viz. Allodpa suctoria 2%. Syngamus trachea with parasitic prevalence of 60%, Capillaria annulata 37.5%, Ascardia galli 24%, Capillaria anatis 40% and Heterakis gallinarum 28.3%. Similarly, two species of trematodes viz. Prosthogonimus ovatus having parasitic prevalence of 50% and Prosthogonimus macrorchis 21% were also documented from fecal avian samples . Single cestode species Raillietina echinobothrida having parasitic prevalence of 72% and 3 protozoan species i.e. Eimeria maxima having parasitic prevalence of 21%, Giardia lamblia 41% and Histomonas meleagridis 18% were documented during corpological analysis. In our recommendation, proper sanitation, medication and vaccination of bird’s enclousres are suggested to avoid parasites.

Resumo Um macho de Pteronura brasiliensis (Carnivora: Mustelidae) foi encontrado morto às margens do Rio Negro, no Pantanal do estado de Mato Grosso do Sul, município de Aquidauana. Dois carrapatos encontrados aderidos à sua pele foram identificados, morfologicamente, como uma ninfa de segundo ínstar de Ornithodoros rostratus (Argasidae) e um macho de Amblyomma sculptum (Ixodidae). Para complementar a identificação morfológica, esses espécimes de carrapatos foram submetidos à extração de DNA e testados por meio de ensaios de PCR, para confirmar a identidade molecular dos espécimes. Além disso, os extraídos dos carrapatos foram testados e revelaram-se negativos nos ensaios de PCR para todos os patógenos testados. Também foram examinados 30 lotes, compostos por 174 indivíduos de O. rostratus, depositados na Coleção Acari do Instituto Butantan, e encontramos material de quatro estados brasileiros, incluindo-se um lote contendo 2 machos e 2 fêmeas de Aquidauana, estado de Mato Grosso do Sul (MS), coletado do solo. Este foi, portanto, o primeiro registro de O. rostratus parasitando P. brasiliensis e o primeiro registro de localidade (Aquidauana). Da mesma forma, A. sculptum é comumente encontrado no Pantanal e relatado aqui, pela segunda vez, parasitando a ariranha, hospedeiro pouco estudado em relação a ectoparasitas. Carnivora (Carnivora Mustelidae Negro Aquidauana identificados morfologicamente Argasidae (Argasidae Ixodidae. Ixodidae . (Ixodidae) morfológica disso revelaramse revelaram se 3 lotes 17 O Butantan brasileiros incluindose incluindo MS, MS , (MS) solo portanto P (Aquidauana) forma A aqui vez ariranha ectoparasitas (Ixodidae 1 (MS (Aquidauana

Abstract A male of Pteronura brasiliensis (Carnivora: Mustelidae) was found dead on the banks of the Rio Negro, in the Pantanal wetlands of Mato Grosso do Sul state, Aquidauana municipality. Two ticks found attached to its skin were morphologically identified as a second-instar nymph of Ornithodoros rostratus (Argasidae) and a male of Amblyomma sculptum (Ixodidae). In order to complement the morphological identification, these tick specimens were subjected to DNA extraction, and tested using PCR assays to confirm the molecular identity the specimens. Also, the tick DNA samples were tested and were negative in the PCR assays for all the pathogens tested. We also examined 30 batches, consisting of 174 individuals of O. rostratus deposited in the Acari Collection of the Butantan Institute, and we found material from four Brazilian states, including one batch containing 2 males and 2 females from Aquidauana, of Mato Grosso do Sul state, collected from the soil. This was therefore the first record of O. rostratus parasitizing P. brasiliensis and the first locality record (Aquidauana). Likewise, A. sculptum is commonly found in the Pantanal and is reported here for the second time parasitizing the giant otter, which is a host little studied regarding the ectoparasites. Carnivora (Carnivora Mustelidae Negro state municipality secondinstar instar Argasidae (Argasidae Ixodidae. Ixodidae . (Ixodidae) identification extraction Also 3 batches 17 O Institute states soil P Aquidauana. (Aquidauana) Likewise otter ectoparasites (Ixodidae 1 (Aquidauana

RESUMO Este estudo investigou a eficácia in vitro do benzoato de emamectina e do glifosato contra monogenéticos de Colossoma macropomum e a tolerância dessa espécie a essas drogas terapêuticas. Ensaios in vitro demonstraram que as concentrações de benzoato de emamectina (12,5; 16,6; 20,8 e 25,0g/L) e de glifosato (2.000; 3.000; 4.000 e 5.000mg/L) foram 100% eficazes contra Anacanthorus spathulatus, Notozothecium janauachensis e Mymarothecium boegeri. As maiores concentrações de benzoato de emamectina (20,8 e 25,0g/L) foram 100% efetivas após 30 minutos de exposição. Para o glifosato, as maiores concentrações foram as mais efetivas, sendo que 4.000 e 5.000 mg/L imobilizaram 100% dos parasitos em 45 minutos e 30 minutos, respectivamente. A microscopia eletrônica de varredura revelou que os parasitos expostos a 20,8 e 25,0g/L de benzoato de emamectina e 5.000 mg/L de glifosato apresentaram tegumento coberto por rugas mais profundas. Alterações comportamentais ocorreram em C. macropomum exposto a todas as concentrações de ambas as drogas, como mortalidade após exposição a benzoato de emamectina e glifosato a 2,0;12,5 e 25,0g/L e 500; 1.000; 2.000; 3.000; 4.000 e 5.000mg/L, respectivamente. Baixas concentrações de benzoato de emamectina e de glifosato foram toleradas por C. macropomum, mas essas baixas concentrações podem não ter eficácia contra infecções por monogenéticos. terapêuticas 12,5 125 12 5 (12,5 16,6 166 16 6 208 20 8 20, 250gL gL 25 0g L g 2.000 2000 2 000 (2.000 3.000 3000 3 4000 4 4.00 5.000mg/L 5000mgL mgL 000mg mg 100 spathulatus boegeri (20, 5000 5.00 respectivamente profundas C 2,012,5 20125 2,0 0 2,0;12, 500 1.000 1000 1 12, (12, 16, 2.00 200 00 (2.00 3.00 300 400 4.0 10 (20 5.0 012 2,012, 2012 2, 2,0;12 50 1.00 (12 2.0 (2.0 3.0 40 4. (2 5. 01 2,012 201 2,0;1 1.0 (1 2. (2. 3. ( 2,01 2,0; 1.

ABSTRACT This study investigated the in vitro efficacy of emamectin benzoate and glyphosate against monogeneans from Colossoma macropomum and tolerance of this species to these therapeutic drugs. In vitro assays demonstrated that concentrations of emamectin benzoate (12.5, 16.6, 20.8 and 25.0g/L) and glyphosate (2,000; 3,000; 4,000 and 5,000mg/L) were 100% effective against Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri. The highest concentrations of emamectin benzoate (20.8 and 25.0g/L) were 100% effective after 30 minutes of exposure. For glyphosate, the highest concentrations were the most effective, and 4,000 and 5,000 mg/L immobilized 100% of parasites in 45 minutes and 30 minutes, respectively. Scanning electron microscopy revealed that the parasites exposed to 20.8 and 25.0g/L of emamectin benzoate and 5,000 mg/L of glyphosate presented integument covered with deeper wrinkles. Behavioral changes occurred in C. macropomum exposed to all concentrations of both drugs, were as mortality occurred following exposure to emamectin benzoate and glyphosate at 2.0,12,5 and 25.0g/L and 500, 1,000, 2,000, 3,000, 4,000 and 5,000mg/L, respectively. Low concentrations of emamectin benzoate and of glyphosate were tolerated by C. macropomum, but these concentrations may have not efficacy against infection of monogeneans. drugs 12.5, 125 12 5 (12.5 166 16 6 16.6 208 20 8 20. 250gL gL 25 0g L g 2,000 2000 2 000 (2,000 3,000 3000 3 4000 4 4,00 5,000mg/L 5000mgL mgL 000mg mg 100 spathulatus boegeri (20. 5000 5,00 respectively wrinkles C 20125 0 2.0,12, 500 1000 1 1,000 12.5 (12. 16. 2,00 200 00 (2,00 3,00 300 400 4,0 10 (20 5,0 2012 2.0,12 50 1,00 12. (12 2,0 (2,0 3,0 40 4, (2 5, 201 2.0,1 1,0 (1 2, (2, 3, ( 2.0, 1, 2.0 2.

ABSTRACT. Ticks are obligate ectoparasite arthropods of the class Arachnida distributed worldwide and can parasitize many animals. They constitute one of the most important groups of vectors of infectious diseases, including zoonoses. The objective of this study was to report the parasitism by Amblyomma ovale Koch, 1844 in a domestic dog (Canis lupus familiaris Linnaeus, 1758) in Santa Maria, in the central region of the state of Rio Grande do Sul, Brazil. Two specimens of ectoparasites found on a domestic dog, living in a rural property, were sent to the Laboratory of Veterinary Parasitology at the Federal University of Santa Maria for taxonomic identification. The two specimens found were classified as adult male and female of A. ovale. Through this study, the parasitism of this species is reported for the first time in the central region of the state of Rio Grande do Sul, Brazil, in a domestic dog, allowing the infestation and transmission of diseases to humans. ABSTRACT animals zoonoses Koch 184 Canis Linnaeus 1758 Sul Brazil property identification A humans 18 175 1 17

BACKGROUND: Parasite infections, unlike regulated animal diseases, do not often receive attention. In fact, parasites are major sources of financial losses in pig enterprises, particularly in subsistence and small-scale pig production systems. OBJECTIVE: To identify and quantify the prevalence of ecto- and endo-parasites among peri-urban free-roaming pigs (FRP) in Gert Sibande District Municipality (GSDM), Mpumalanga. METHOD: Pig owners were identified using the snowball sampling method since no sampling frame for FRP farmers exists. Stratified sampling was used to select pigs for sampling for ecto- and endo-parasites. A form was used to record the observations. Pairwise correlation analysis was performed using Stata 15.0. The SPSS V28.0 statistical package was used to perform the chi-square test (X²) to assess the distribution of parasites in different age groups. The prevalence of parasites was assessed in different age groups of pigs using multi-response crosstabs. Multivariate analysis of variance (MANOVA) was used to estimate the marginal mean of parasites according to municipality. Statistical significance was assessed at α < 0.05. RESULTS: Over 90% (91.2%) of the pigs examined were infested with at least one parasite, including Haematopinus suis, Sarcoptes scabiei, Ascaris suum, Fasciola hepatica, Trichuris suis, Strongylids, Coccidia spp, Moniezia expansa, and Siphonaptera spp. The correlation between Ascaris suum and body condition was weak but statistically significant (r = 0.24; p < 0.05. CONCLUSION: Policy makers, animal researchers and veterinary services must focus on developing policies, risk communication and community engagement materials, which target pig farmers in peri-urban areas such as Gert Sibande District Municipality, Mpumalanga Province.

Resumo A fauna de carrapatos do Brasil compreende, atualmente, 77 espécies válidas, categorizadas em duas famílias: Ixodidae (53 espécies) e Argasidae (24 espécies). No estado de Alagoas, apenas seis carrapatos ixodídeos foram registrados até o momento, sem nenhum relato anterior de argasídeos. Neste estudo, 33 gambás-de-orelha-branca (Didelphis albiventris Lund, 1840), resgatados na região metropolitana de Maceió, foram encaminhados ao Centro de Triagem de Animais Silvestres (Cetas) da cidade. Ao chegarem, os animais foram submetidos a exames de ectoparasitas em um prazo de 24 horas. Do total, 10/33 (30%) deles foram encontrados infestados por 26 larvas do carrapato argasídeo Ornithodoros mimon Kohls, Clifford & Jones, 1969. A identificação morfológica dos carrapatos foi corroborada pela obtenção de sequências parciais do gene mitocondrial 16S rRNA de três espécimes deles. Este estudo marca o primeiro relato de um carrapato argasídeo no estado de Alagoas. Estudos futuros devem investigar se as populações de ambos os carrapatos, O. mimon e seu hospedeiro, D. albiventris, no estado de Alagoas, carregam potenciais agentes zoonóticos capazes de causar doenças transmitidas por carrapatos. compreende atualmente 7 válidas famílias 53 (5 (2 espécies. . Alagoas momento argasídeos 3 gambásdeorelhabranca gambás orelha branca Didelphis Lund 1840, 1840 , 1840) Maceió Cetas (Cetas cidade chegarem 2 horas total 1033 10 10/3 30% 30 (30% Kohls Jones 1969 S O hospedeiro D 5 ( 184 103 1 10/ (30 196 18 (3 19

Abstract The Brazilian tick fauna currently comprises 77 valid species categorized into two families: Ixodidae (53 species) and Argasidae (24 species). In the state of Alagoas, only six Ixodid ticks have been reported to date, with no previous reports of ticks in the Argasidae family. Here, we assessed 33 White-eared Opossum (Didelphis albiventris Lund, 1840) rescued in the metropolitan region of Maceió and referred to the Wild Animal Screening Center (Cetas) in the city. Upon arrival, the animals were examined for ectoparasites within 24 hours. In total, 10/33 (30%) opossums were found to be infested by 26 larvae of the argasid tick Ornithodoros mimon Kohls, Clifford & Jones, 1969. Morphological identification of ticks was corroborated by generating partial sequences of the mitochondrial 16S rRNA gene from three tick specimens. This study marks the first report of an argasid tick in the state of Alagoas. Future studies should investigate whether populations of both O. mimon ticks and their host, D. albiventris, in the state of Alagoas carry potential zoonotic agents capable of causing tick-borne diseases. 7 families 53 (5 (2 species. . date family Here 3 Whiteeared White eared Didelphis Lund 1840 Cetas (Cetas city arrival 2 hours total 1033 10 10/3 30% 30 (30% Kohls Jones 1969 S specimens O host D tickborne borne diseases 5 ( 184 103 1 10/ (30 196 18 (3 19

Abstract Fish parasites are an important part of aquatic biodiversity and knowing these species and their interactions with their hosts helps in monitoring the aquatic biota. The present study investigated the ectoparasite crustacean fauna of ten fish species from the upper Araguari River, in the state of Amapá, northern Brazil. A total of 508 fish were collected and analyzed from July to November 2014, of which 82.6% (109) were parasitized by one or more crustacean ectoparasite species. In the ten host fish species, a total of 308 ectoparasite specimens were collected, from 12 taxa, such as Argulus multicolor Stekhoven, 1937, Argulus spinulosus Silva, 1980, Argulus sp.1, Argulus sp.2, Argulus sp.3, Dipteropeltis sp., Dipteropeltis hirundo Calman, 1912, Dolops bidentata Bouvier, 1899, Dolops striata Bouvier, 1899 (Argulidae), Braga fluviatilis Richardson, 1911, Braga amapaensis Thatcher, 1996 (Cymothoidae) and Excorallana berbicensis Boone, 1918 (Corallanidae). Higher levels of prevalence and abundance were recorded for Hoplias aimara (Valenciennes, 1847) and Tometes trilobatus Valenciennes, 1850, respectively. These ectoparasites were found in the fins, integument, mouth, and anus of the host fish. Argulus sp.2 and D. bidentata were the most abundant parasites (65.1%), and had the highest species richness. This study registered 36 novel host-parasite interactions, and thus represents a new record for all host species here examined. biota River Amapá Brazil 50 2014 826 82 6 82.6 109 (109 30 1 taxa Stekhoven 1937 Silva 1980 sp1 sp sp.1 sp2 2 sp3 3 sp.3 sp. Calman 1912 Bouvier 189 Argulidae, Argulidae , (Argulidae) Richardson 1911 Thatcher 199 Cymothoidae (Cymothoidae Boone 191 Corallanidae. Corallanidae . (Corallanidae) Valenciennes (Valenciennes 1847 1850 respectively fins integument mouth D 65.1%, 651 65.1% 65 (65.1%) richness hostparasite parasite examined 5 201 8 82. 10 (10 193 198 18 (Argulidae 19 (Corallanidae 184 185 65.1 (65.1% 20 (1 65. (65.1 ( (65. (65 (6

Abstract This study investigated the spatial distribution, parasite interactions, and affinities of Branchiura parasites according to macrohabitat and surface type on fish from the Araguari River, Amapá State, in the Brazilian Amazon. This study presents novel information on the interactions of branchiuran species with host fish, and how the morphology of their attachment structures is associated with different host surface morphologies, with and without scales. A total of 558 fish specimens of 26 species were collected, of which 109 fish (10 species) were parasitized by at least one branchiuran parasite; these were selected for further study. The parasites were collected from the buccal cavity, gills, fins, and integument of the host fish. Parasites with suction discs (Argulus) were mainly associated to hosts with small scales and no scales, while parasites with hooks (Dolops) were mainly associated to hosts with large scales. Overall, parasites with suction discs were more specialized per site than those with hooks. distribution River State Amazon morphologies 55 2 10 (1 cavity gills fins Argulus (Argulus Dolops (Dolops Overall 5 1 (

Abstract Isopods of the suborder Cymothoida include ectoparasite species that cause physical damage and affect the development of the host by feeding on blood and tissues. On fishes, these parasites attach themselves to the skin, fins, gills, and oral cavity using claws on articulated legs. The ability to remain hidden in cavities even after the fish is caught enables isopods to be transported to new areas along with the host, making these parasites potential co-invaders. Understanding the role and environmental impact of parasites is essential, although research on parasitism in the context of biological invasions lags in comparison to general research on such invasions. Previous records indicate only two species of parasitic isopods in the São Francisco River basin. However, the present study reports the first record of two other species (Braga patagonica and Braga nasuta) on Pygocentrus piraya and in the basin itself. This demonstrates a lack of knowledge regarding fish parasites in the region, despite the presence of other parasites. Collaborations with research groups conducting fish sampling in the basin can contribute to expanding knowledge on these parasites and the training of human resources in fish parasitology. The present study provides important information on the presence of unidentified parasitic isopods on fishes in the São Francisco River basin, underscoring the need for further studies and expanding the geographic distribution of these parasites, as well as the host list for these isopods. tissues skin fins gills legs coinvaders. coinvaders co invaders. invaders co-invaders essential However nasuta itself region parasitology

Resumo O camarão-da-amazônia ou Macrobrachium amazonicum (Heller, 1862) é amplamente distribuído na América do Sul, ocorrendo nos rios Orinoco e Amazonas, importante fonte renda de familias riberinhas. Esse camarão é hospedeiro de ectoparasitas crustáceos do gênero Probopyrus (Giard & Bonnier, 1888) (Bopyridae) que infestam a cavidade branquial da espécie. O presente estudo tem o objetivo de relatar novas ocorrências de ectopasitas do gênero Probopyrus no camarão-da-amazônia, capturados no rio Amazonas. Macrobrachium amazonicum foram coletados no período de maio de 2017 e abril de 2018, e novamente de julho de 2021 a maio de 2022, nas regiões da Ilha de Santana e Rio Mazagão, estado do Amapá, Brasil. Entre 5.179 exemplares de camarões capturados, 133 foram parasitados sendo quatro pelos ectoparasitas Probopyrus pandalicola (Packard, 1879), Probopyrus bithynis (Richardson, 1904), Probopyrus floridensis (Richardson, 1904) e Probopyrus palaemoni (Lemos de Castro & Brasil Lima, 1974). Registrado pela primeira vez a ocorrência no M. amazonicum por P. floridensis e P. palaemoni no litoral norte do Brasil. Esses quatro ectoparasitos não se limitam a espécies ou gêneros hospedeiros específicos, observados nesse estudo que relata quatro espécies de Probopyrus infestando M. amazonicum. camarãodaamazônia amazônia Heller, Heller (Heller 1862 Sul Amazonas riberinhas Giard Bonnier 1888 Bopyridae (Bopyridae espécie camarãodaamazônia, amazônia, 201 2018 202 2022 Mazagão Amapá 5179 5 179 5.17 13 Packard, Packard (Packard 1879, 1879 , 1879) Richardson, Richardson (Richardson 1904, 1904 Lemos Lima 1974. 1974 . 1974) M P específicos 186 188 20 517 17 5.1 1 187 190 197 18 2 51 5. 19

Abstract The Amazon prawn or Macrobrachium amazonicum (Heller, 1862) is widely distributed in South America, occurring in the Orinoco and Amazon rivers, and forms an important source of income for riverside families. This prawn hosts crustacean ectoparasites of the genus Probopyrus (Giard & Bonnier, 1888) (Bopyridae) that infest its gill cavity. The aim of the present study was to report new occurrences of Probopyrus in Amazon prawns caught in the Amazon River. Macrobrachium amazonicum prawns were collected between May 2017 and April 2018, and again from July 2021 to May 2022 in the regions of Ilha de Santana and Rio Mazagão, state of Amapá, Brazil. Among the 5,179 prawn specimens caught, 133 were parasitized by the ectoparasites Probopyrus pandalicola (Packard, 1879), Probopyrus bithynis (Richardson, 1904), Probopyrus floridensis (Richardson, 1904) and Probopyrus palaemoni (Lemos de Castro & Brasil Lima, 1974). These occurrences of P. floridensis and P. palaemoni in M. amazonicum were the first records of this on the northern coast of Brazil. These four ectoparasites are not limited to specific host species or genera, as observed in this study, which reports four species of Probopyrus infesting M. amazonicum. Heller, Heller (Heller 1862 America rivers families Giard Bonnier 1888 Bopyridae (Bopyridae cavity River 201 2018 202 Mazagão Amapá Brazil 5179 5 179 5,17 13 Packard, Packard (Packard 1879, 1879 , 1879) Richardson, Richardson (Richardson 1904, 1904 Lemos Lima 1974. 1974 . 1974) P M genera 186 188 20 517 17 5,1 1 187 190 197 18 2 51 5, 19