RESUMO Amostras de sangue e fezes de perdiz chukar (Alectoris chukar), faisão-albino (Phasianus colchicus), faisão-prateado (Lophura nycthemera), periquito-de-rosa (Psittacula krameri) e perus (Meleagris gallopavo) foram analisadas para verificar a prevalência de parasitas. Para registrar os parasitas, essas cinco espécies de aves foram colocadas em gaiolas separadas no Centro de Conservação e Pesquisa de Aves, Departamento de Vida Selvagem e Ecologia, Universidade de Veterinária e Ciências Animais, Lahore, Paquistão. Cem amostras fecais e 100 amostras de sangue para cada espécie de ave foram inspecionadas para analisar os parasitas internos. Durante o presente estudo, foram examinadas 17 espécies de endoparasitas, 14 de amostras fecais e 3 de sangue. Foram estudadas duas espécies de ectoparasitas, ou seja, o ácaro Dermanyssus gallinae 42% e o carrapato aviário Args persicus 41%. Os parasitas sanguíneos incluíram Plasmodium juxtanucleare 50%, Leucoctoyzoon simond com prevalência parasitária de 40% e Aegyptinella pullorum com prevalência parasitária de 40%. As espécies parasitas registradas em amostras fecais incluíram 6 espécies de nematoides viz. Allodpa suctoria 2%, Syngamus traqueia com prevalência parasitária de 60%, Capillaria annulata 37,5%, Ascardia galli 24%, Capillaria anatis 40% e Heterakis gallinarum 28,3%. Da mesma forma, duas espécies de trematódeos viz. Prosthogonimus ovatus com prevalência parasitária de 50% e Prosthogonimus macrorchis 21% também foram documentados em amostras fecais de aves. Espécies de cestoide único Raillietina echinobothrida com prevalência parasitária de 72% e 3 espécies de protozoários, isto é, Eimeria maxima com prevalência parasitária de 21%, Giardia lamblia 41% e Histomonas meleagridis 18% foram documentadas durante a análise corpológica. Em nossa recomendação, o saneamento adequado, medicação e vacinação de invólucros de pássaros são sugeridos para evitar parasitas.

Abstract Blood and fecal samples of chukar partridge (Alectoris chukar), albino pheasant (Phasianus colchicus), silver pheasant (Lophura nycthemera), rose-ringed parakeet (Psittacula krameri) and turkeys (Meleagris gallopavo) were analyzed to check parasitic prevalence. To record parasites these five avian species were placed kept in separate cages at Avian Conservation and Research Center, Department of Wildlife an Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan. 100 fecal and 100 blood samples for each bird species were inspected to analyze internal parasites. During present study, 17 species of endoparasites 14 from fecal samples and three from blood were examined. Two species of ectoparasites i.e. mite Dermanyssus gallinae 42% and fowl ticks Args persicus 41%were studied. Blood parasites included Plasmodium juxtanucleare 50%, Leucoctoyzoon simond having parasitic prevalence 40%, and Aegyptinella pullorum having parasitic prevalence of 40%. Parasitic species recorded from fecal samples included 6 species of nematodes viz. Allodpa suctoria 2%. Syngamus trachea with parasitic prevalence of 60%, Capillaria annulata 37.5%, Ascardia galli 24%, Capillaria anatis 40% and Heterakis gallinarum 28.3%. Similarly, two species of trematodes viz. Prosthogonimus ovatus having parasitic prevalence of 50% and Prosthogonimus macrorchis 21% were also documented from fecal avian samples . Single cestode species Raillietina echinobothrida having parasitic prevalence of 72% and 3 protozoan species i.e. Eimeria maxima having parasitic prevalence of 21%, Giardia lamblia 41% and Histomonas meleagridis 18% were documented during corpological analysis. In our recommendation, proper sanitation, medication and vaccination of bird’s enclousres are suggested to avoid parasites.

RESUMO Este estudo teve como objetivo caracterizar a epidemiologia da presença de ácaros hematófagos em granjas de postura no estado de Minas Gerais. Foi utilizado um banco de dados secundário, com informações de 402 galpões de 42 propriedades comerciais. As variáveis utilizadas para compor o modelo de correspondência foram selecionadas por meio do teste qui-quadrado (P≤0,05). Foi construído um índice para a presença de ácaros hematófagos, considerando alguns fatores de risco. Além disso, um estudo da análise espacial foi realizado para avaliar a presença de ácaros hematófagos em Minas Gerais. Observou-se a presença de ácaros hematófagos em 48% dos galpões, sendo a de O. sylviarum de 45,5%, O. bursa de 17,4% e D. gallinae de 2,7%. Houve associação entre o índice de risco com os intervalos de remoção de fezes e com a presença ou a ausência de aves sinantrópicas. Verificou-se que granjas mais tecnificadas não utilizam acaricida em seus galpões. A presença de ácaros hematófagos foi observada na mesorregião Sul/Sudoeste de Minas Gerais e entre as mesorregiões Oeste de Minas e Metropolitana de Belo Horizonte. Esses resultados fornecem conhecimento sobre a epidemiologia desses ectoparasitos e podem contribuir na tomada de decisões, reduzindo os riscos de possíveis infestações em aves de postura.

ABSTRACT This study aimed to characterize the epidemiology of the presence of hematophagous mites in posture farms in the state of Minas Gerais. Was used a secondary database with information from 402 chicken houses in 42 commercial properties. The variables used to compose the correspondence model were selected through chi-square tests (P≤0.05). A risk index was built to the presence of hematophagous mites considering some risk factors. Additionally, a study of the spatial analysis was done to evaluate the presence of hematophagous mites in Minas Gerais. Was observed the presence of hematophagous mites in 48% of chicken houses, the presence of O. sylviarum was 45.5%, O. bursa 17.4% and D. gallinae 2.7%. There was an association between the risk index with the intervals for the removal of manure and the presence or absence of synanthropic birds. It was verified that more technified farms do not use acaricide in their chicken houses. The presence of hematophagous mites was observed in the Messoregions South/Southwest of Minas Gerais and between the West and Metropolitan Mesoregion of Belo Horizonte. These results provide knowledge about the epidemiology of these ectoparasites and may contribute to decision making by reducing the risks of possible infestations in poultry.

ABSTRACT The aim of this study was to determine the prevalence of ectoparasites in guinea pigs (Cavia porcellus) of family-commercial breeding in the district of Matahuasi, Junín, Peru, as well as to identify parasitic species, type of parasitic association and evaluate the association between the presence of ectoparasites and the productive stage and sex variables. A total of 299 guinea pigs were evaluated between January and March 2017. Ectoparasites were collected using four techniques (deep skin scraping, adhesive tape, trichogram and fine combing). The ectoparasites were examined by direct observation under the microscope at 10X and 40X, and in some cases they were rinsed with 10% KOH. The prevalence of ectoparasites was 67.0 ± 5.3%, identifying three species of acariforms (63.0 ± 5.5%) and one species of Phthiraptera (12.0 ± 3.7%). Among the acariforms, Ornithonyssus bursa (53%), Chirodiscoides caviae (15%) and Dermanyssus gallinae (7%) were identified. The Phthiraptera species was Gliricola porcelli (12%). Monoparasitism was the most frequent (75%). No significant association was found between the presence of ectoparasites and the productive stage and sex variables.

RESUMEN El objetivo del estudio fue determinar la prevalencia de ectoparásitos en cuyes (Cavia porcellus) de crianza familiar-comercial en el distrito de Matahuasi, Junín, Perú, así como identificar las especies parasitarias, tipo de asociación parasitaria y evaluar la asociación entre la presencia de ectoparásitos y las variables etapa productiva y sexo. Se evaluaron 299 cuyes entre enero y marzo de 2017. Los ectoparásitos fueron recolectados mediante cuatro técnicas (raspado profundo de piel, cinta adhesiva, tricograma y peinado fino). Los ectoparásitos fueron examinados por observación directa al microscopio a 10X y 40X, y en algunos casos fueron aclarados con KOH al 10%. Se encontró una prevalencia de 67.0 ± 5.3%, identificándose tres especies de acariformes (63.0 ± 5.5%) y una especie de Phthiraptera (12.0 ± 3.7%). Dentro de los acariformes se identificó Ornithonyssus bursa (53%), Chirodiscoides caviae (15%) y Dermanyssus gallinae (7%). La especie Phthiraptera fue Gliricola porcelli (12%). El monoparasitismo fue el más frecuente (75%). No se encontró asociación significativa entre la presencia de ectoparásitos y las variables etapa productiva y sexo.

Resumen El acaro rojo Dermanyssus gallinae afecta las producciones avicolas, principalmente de ponedoras, al alimentarse de su sangre. Su control se realiza a través de acaricidas químicos, hacia los cuales aumenta su resistencia. Se evaluo la actividad acaricida de aceite esencial (ae) de cilantro (Coriandrum sativum) a concentraciones de 1, 2 y 3 mg/cm2 a través de bioensayos por contacto sobre adultos de D. gallinae y se comparo con un acaricida químico (Neguvon*). Los ácaros fueron colectados de aves (Gallus gallus L.) de traspatio. Se realizaron seis repeticiones por tratamiento. Estadísticamente, se determinaron los porcentajes de mortalidad y se calculó el anova. Se estableció el porcentaje de la actividad letal. Se estimo la CL50 y el TL50. El ae de cilantro causo el 100 % de mortalidad sobre la población de D. gallinae a los 25 min después del contacto con una concentración de 2 y 3 mg/cm2 y a los 30 min con una concentración de 1 mg/cm2. La CL50 del ae de cilantro fue de 1,3 mg /cm2, con un TL50 de 13 min sobre la población de D. gallinae. Se sugiere el aceite esencial de cilantro C. sativum como una alternativa a los acaricidas químicos para el control del acaro rojo de aves D. gallinae.

Abstract The red mite Dermanyssus gallinae affects poultry production mainly of laying hens by feeding on its blood. Its control is carried out using chemical acaricides, toward which its resistance increases. The aim of this study was to evaluate the acaricidal activity of essential oil (eo) of coriander (Coriandrum sativum) at concentrations of 1, 2 and 3 mg/cm2 through contact bioassays on adults of D. gallinae, compared to a chemical acaricide (Neguvon*). The mites were collected from backyard hens (Gallus gallus L.). Six replicates were performed per treatment. Statistically, mortality percentages were established and an anova was carried out. The lethal activity percentage was established. Additionally, the median lethal concentration (LC50) and the median lethal time (TL50) were estimated. Coriander eo caused 100 % mortality on D. gallinae population 25 min after contact with a concentration of 2 and 3 mg/cm2 and after 30 min with a lower concentration of 1 mg/cm2. The LC50 of the coriander eo was 1.3 mg/cm2, with a 13 min TL50 on the D. gallinae population. The eo of coriander is suggested as an alternative to be used instead of the chemical acaricides for the control of the red poultry mite D. gallinae.

The poultry red mite, Dermanyssus gallinae, is considered as a limiting factor in poultry production. In this study we evaluated the effect of Bacillus thuringiensis var. Kurstaki (Btk) on larvae of poultry red mite. The contact bioassay method on larvae of D. gallinae per experimental unit was used. The assessed concentrations of Btk were: 15, 25, 35 and 45 mg/ml. Observations were made every 24 hours for 7 days. A moderate mortality of D. gallinae was observed from day 2 of application (66%), which increased up to 78% at 7 days at a concentration of 35 mg/ml. Tukey's mean (95% confidence) did not show significant differences (p≥0.05) between the mortality of D. gallinae nymphs produced by the concentrations 25, 35 and 45 mg/ml, while concentrations of 0 and 15 mg/ml did have a killing effect. A LC50 of 17.8 mg/ml was determined. Btk caused damage on the cuticle of D. galllinae and loss of mobility of the poultry red mite in a period of 24 hours. Btk may be considered as an alternative control method to chemical acaricides for the control of D. gallinae in integrated management programs.

El ácaro rojo Dermanyssus gallinae es limitante en la producción de aves de corral. En este estudio se evaluó el efecto de Bacillus thuringiensis var.kurstaki (Btk) sobre larvas del ácaro rojo. Se utilizó el método de bioensayos por contacto sobre larvas de D. gallinae por unidad experimental. Las concentraciones evaluadas de Btk fueron: 15, 25, 35 y 45 mg/ml. Se realizaron observaciones cada 24 horas durante 7 días. Se observó una mortalidad moderada de D. gallinae a partir del segundo día de aplicación de la bacteria (66%) y al séptimo día aumentó a 78% a 35 mg/ml de concentración. La comparación de medias de Tukey (95% de confianza) no presentó diferencias significas (p≥0,05) entre la mortalidad de las ninfas de D. gallinae producida por las concentraciones 25, 35 y 45 mg/ml, mientras que sí se presentaron cuando se utilizaron concentraciones de 0 y 15 mg/ml. La CL50 obtenida fue de 17,8 mg/ml. Btk causó daños sobre la cutícula de D. galllinae y generó pérdida de la movilidad del ácaro rojo en un periodo de 24 horas, por lo cual puede ser considerado como un método de control alternativo a los acaricidas químicos para el control de D. gallinae en programas de manejo integrado.

ABSTRACT Poultry red mite Dermanyssus gallinae is a cosmopolitan and hematophagous species commonly found in layer houses around the world. Poultry mite infestations may cause anemia, stress, low body weight and egg production, and mortality. Mite control is typically based on chemical products, but they are not effective and leave residues in eggs; therefore, alternative control methods, such as entomopathogenic fungi, need to researched. This study aimed at evaluating, in the laboratory, the activity of Brazilian isolates of entomopathogenic fungi against D. gallinae. The mites were collected from a commercial layer house and were sprayed with conidial suspensions (1 × 108 conidia/mL) of five isolates of Beauveria bassiana and Metarhizium anisopliae. All tested isolates were pathogenic for the red mite, with confirmed mortality ranging from 22.9 to 52.4%. This demonstrate the potential of the tested entomopathogenic fungi isolates for mite control, and reinforces the need for further studies with other isolates, application strategies, and with fungal formulations.

Global poultry production is plagued by a wide variety of arthropods. The problems associated with their chemical control have led to an increasing search for control alternatives, and entomopathogenic fungi seem to be a promising strategy. Despite the large number of insects and mites considered as important pests in animal production, studies on the use of entomopathogenic fungi for their control are still scarce compared with agricultural pests, particularly in Brazil. This article reviews some damages and control aspects of the main arthropod pests that affect Brazilian poultry production, including house flies, lesser mealworms, and feather mites, by the use of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae. Studies published in the last 20 years were reviewed, and the main problems and limitations of that pest-control strategy are discussed.

O presente trabalho teve como objetivo comparar a eficiência de armadilhas de papel corrugado com a de armadilhas de colmo de bambu taquaril (Phyllostachys sp.) seco na captura de Dermanyssus gallinae, presentes em uma bateria de gaiolas de metal utilizadas na criação de galinhas de postura. A presença de ovos no interior de cada tipo de armadilha foi comparada por meio do teste de Qui-Quadrado e pelo intervalo de confiança para duas proporções. Os valores das somas de estágios móveis nas diferentes armadilhas a cada intervalo de tempo foram comparados utilizando-se o teste de Wilcoxon. A proporção de armadilhas contendo ovos não foi diferente nos dois tipos de armadilha (p < 0,05). O número total de estágios móveis capturados durante a coleta a cada dois dias de intervalo em cada tipo de armadilha foi diferente (p < 0,01), enquanto que as contagens, nas armadilhas de papelão, apresentaram distribuição normal e foram superiores aos valores de espécimes móveis capturados nas armadilhas de bambu.

This work aims to compare the performance of corrugated paper and "taquaril" bamboo (Phyllostachys sp.) straw traps for collecting (in sampling) Dermanyssus gallinae in a metal cages battery laying hens. The presence of eggs in the two trap models were compared using a Qui-square test and a proportion confidence interval test. Total daily values of mobile instars gathered in each type of trap were compared using the Wilcoxon's test. The amount of traps containing eggs was not different in neither of the traps (p < 0,05). The number of mobile instars sampled at every two days per trap model was different (p < 0,01). Counting in the corrugated paper traps showed a Gauss distribution histogram and was superior to the values of the bamboo straw traps.

RESUMO O trabalho teve por objetivo estudar a influência do jejum na fecundidade de Dermanyssus gallinae. Fêmeas foram colocadas em tubos para se alimentar sobre uma ave jovem e, em seguida mantidas sob jejum por períodos de 1 a 12 dias. Os resultados obtidos demonstraram que as fêmeas ovipuseram até o 11º dia. O número de ovos depositados variou de 1 a 8, sendo as maiores médias obtidas nas fêmeas mantidas até o 8° dia de jejum. A maior porcentagem de oviposição ocorreu até o terceiro dia de jejum, havendo uma redução do 4° ao 12° dia.

ABSTRACT The objective of the present study was to evaluate the influence of fasting on the fecundity of D. gallinae. Females were maintained in tubes in order to feed on a young bird and then were kept fasting for 1 to 12 days. The results demonstrated that females laid eggs up to the 11th day. The number of eggs laid ranged from 1 to 8, and the greatest means were obtained in females kept up to 8 days fasting. The greatest percentage of eggs laid occurred up to the 3rd day of fasting, with a decrease from the 4th to the 12th day.

Experimentos de laboratório foram realizados visando estimar as exigências térmicas de Dermanyssus gallinae. Para isso, o desenvolvimento do ácaro foi estudado em condições de laboratório usando-se câmaras climatizadas reguladas a 15, 20, 25, 30 e 35°C, fotofase de 12h e UR de 6085%. As exigências térmicas determinadas para D. gallinae foram: Pré-oviposição: temperatura base de 3,4 ºC, constante térmica (k) igual a 562,85 graus-hora, coeficiente de determinação (R²) igual a 0,59, equação de regressão: Y= -0,006035 + 0,001777 x. Ovo. Temperatura base de 10,60 ºC, constante térmica (k) igual a 689,65 graus-hora, coeficiente de determinação (R²) igual a 0,94, equação de regressão: Y= -0,015367 + 0,001450 x. Larva. Temperatura base 9,82 ºC, constante térmica (k) igual a 464,91 graus-hora, R² igual a 0,87, equação de regressão: Y= -0,021123+0,002151x. Protoninfa. Temperatura de 10,17 ºC, constante térmica (k) igual a 504,49 graus-hora, coeficiente de determinação (R²) igual a 0,90, equação de regressão: Y= -0,020152 + 0,001982 x. Deutoninfa. Temperatura de 11,80 ºC, constante (k) térmica igual a 501,11 graus-hora, coeficiente de determinação (R²) igual a 0,99, equação de regressão: Y= -0,023555 + 0,001996 x. Os resultados obtidos permitem concluir que no Estado de São Paulo podem ocorrem de 15 a 42 gerações/ano de D. gallinae, em estimativa baseada em isotermas. Dermanyssus gallinae pode se desenvolver continuamente no Estado de São Paulo, com diminuição da população no inverno. Existem diferenças entre os estágios de desenvolvimento de D. gallinae com relação às exigências térmicas.

The thermal requirements for development of Dermanyssus gallinae were studied under laboratory conditions at 15, 20, 25, 30 and 35°C, a 12h photoperiod and 60-85% RH. The thermal requirements for D. gallinae were as follows. Preoviposition: base temperature 3.4ºC, thermal constant (k) 562.85 degree-hours, determination coefficient (R²) 0.59, regression equation: Y= -0.006035 + 0.001777 x. Egg: base temperature 10.60ºC, thermal constant (k) 689.65 degree-hours, determination coefficient (R²) 0.94, regression equation: Y= -0.015367 + 0.001450 x. Larva: base temperature 9.82ºC, thermal constant (k) 464.91 degree-hours, determination coefficient R² 0.87, regression equation: Y= -0.021123+0.002151 x. Protonymph: base temperature 10.17ºC, thermal constant (k) 504.49 degree-hours, determination coefficient (R²) 0.90, regression equation: Y= -0.020152 + 0.001982 x. Deutonymph: base temperature 11.80ºC, thermal constant (k) 501.11 degree-hours, determination coefficient (R²) 0.99, regression equation: Y= -0.023555 + 0.001996 x. The results obtained showed that 15 to 42 generations of Dermanyssus gallinae may occur during the year in the State of São Paulo, as estimated based on isotherm charts. Dermanyssus gallinae may develop continually in the State of São Paulo, with a population decrease in the winter. There were differences between the developmental stages of D. gallinae in relation to thermal requirements.

RESUMO O presente trabalho teve por objetivo estudar a fecundidade de Dermanyssus gallinae em condições de laboratório. As fêmeas foram isoladas em pipetas "Pasteur" e mantidas em estufa climatizada sob 27° C, 80%UR e 12:12 (C:E). As avaliações foram feitas no terceiro dia após a alimentação, ocasião em que foi oferecida às fêmeas uma nova alimentação. Esta etapa foi repetida a cada 3 dias até o final do experimento. As avaliações foram feitas anotando-se o número total de ovos colocados após cada alimentação. As fêmeas iniciaram a oviposição logo após o 1o repasto sanguíneo e continuaram ovipondo após 7 alimentações. A maior porcentagem das fêmeas (58,1%) ovipôs após a primeira alimentação. A partir do segundo repasto esta porcentagem foi diminuindo até a oitava alimentação, onde se recuperou apenas uma fêmea, que morreu antes de ovipor. No presente experimento as fêmeas de D. gallinae foram capazes de realizar um total de 7 oviposições com uma produção total de 25 ovos. O padrão de oviposição de D. gallinae é do tipo declinante, ocorrendo um aumento no número de ovos após cada repasto até atingir um número máximo (3a alimentação) e decrescendo nas alimentações subseqüentes.

ABSTRACT The purpose of this work was to study the fecundity and the fertility of Dermanyssus gallinae under laboratory conditions. Females were maintained at 27° C, 80%UR and 12:12 (C:E). Observations were made on 3rd day after a blood meal when the total number of eggs was evaluated and a new blood meal was offered. The results showed that the females began to lay eggs just after the first blood meal and continued to lay eggs up to the 7th day after the meal. The highest percentage of females (58%) laid eggs after the first meal. After thesecond meal this percentage began to decrease until the8th meal, when only one female was present, but it died before it was able to lay eggs. Dermanyssus gallinae females had a total of 7 oviposition cycles totaling 25 eggs.

RESUMO A ação acaricida sobre Dermanyssus gallinae e o teor de cafeina foram determinados em extratos clorofórmicos, etanólicos e aquosos de folhas secas de espécies de Coffea. Forte atividade acaricida foi observada nos extratos clorofórmicos de C. racemosa (100%), C. canephora (100%), C. salvatrix (82%), C. stenophylla (73%), C. arabica (83%) e no extrato etanólico de C. canephora (90%). Outros extratos etanólicos demonstraram moderada atividade contra D. gallinae (45-62%). Os extratos aquosos demonstraram fraca atividade acaricida. Os maiores teores de cafeína foram observados nos extratos clorofórmicos de C. arabica (0,436%), C. canephora (0,246%), C. stenophylla (0,132%), C. liberica (0,127%) e extratos etanólicos de C. arabica (0,131%) e C. canephora (0,077%). Testes com soluções de cafeína (0,01 - 1,0%) não demonstraram ação acaricida efetiva. Coffea C 100%, 100 100% , (100%) 82%, 82 82% (82%) 73%, 73 73% (73%) 83% 83 (83% 90%. 90 90% . (90%) D 4562%. 4562 45 62% 62 (45-62%) 0,436%, 0436 0,436% 0 436 (0,436%) 0,246%, 0246 0,246% 246 (0,246%) 0,132%, 0132 0,132% 132 (0,132%) 0,127% 0127 127 (0,127% 0,131% 0131 131 (0,131% 0,077%. 0077 0,077% 077 (0,077%) 0,01 001 01 (0,0 1,0% 10 1 efetiva (100% 8 (82% 7 (73% (83 9 (90% 4562% 456 4 6 (45-62% 043 0,436 43 (0,436% 024 0,246 24 (0,246% 013 0,132 13 (0,132% 0,127 012 12 (0,127 0,131 (0,131 007 0,077 07 (0,077% 0,0 00 (0, 1,0 (100 (82 (73 (8 (90 (45-62 04 0,43 (0,436 02 0,24 2 (0,246 0,13 (0,132 0,12 (0,12 (0,13 0,07 (0,077 0, (0 1, (10 (7 ( (9 (45-6 0,4 (0,43 0,2 (0,24 0,1 (0,1 (0,07 (1 (45- (0,4 (0,2 (45 (4

ABSTRACT Chloroformic, ethanolic and aqueous extracts of dried leaves of Coffea species were assessed for acaricide activity against Dermanyssus gallinae and caffeine content was measured. Strong acaricide activity was observed on chloroformic extracts of C. racemosa (100%), C. canephora (100%), C. salvatrix (82%), C. stenophylla (73%), C. arabica (83%) and ethanolic extract of C. canephora (90%). Others ethanolic extracts showed moderate activity against D. gallinae (45-62%). The aqueous extracts showed weak activity. Higher caffeine content were observed on chloroformic extracts of C. arabica (0.436%), C. canephora (0.246%), C. stenophylla (0.132%), C. liberica (0.127%) and ethanolic extracts of C. arabica (0.131%) e C. canephora (0.077%). Tests with caffeine solutions (0.01 - 1.0%) no demonstrated effective acaricidal activity. Chloroformic measured C 100%, 100 100% , (100%) 82%, 82 82% (82%) 73%, 73 73% (73%) 83% 83 (83% 90%. 90 90% . (90%) D 4562%. 4562 45 62% 62 (45-62%) 0.436%, 0436 0.436% 0 436 (0.436%) 0.246%, 0246 0.246% 246 (0.246%) 0.132%, 0132 0.132% 132 (0.132%) 0.127% 0127 127 (0.127% 0.131% 0131 131 (0.131% 0.077%. 0077 0.077% 077 (0.077%) 0.01 001 01 (0.0 1.0% 10 1 (100% 8 (82% 7 (73% (83 9 (90% 4562% 456 4 6 (45-62% 043 0.436 43 (0.436% 024 0.246 24 (0.246% 013 0.132 13 (0.132% 0.127 012 12 (0.127 0.131 (0.131 007 0.077 07 (0.077% 0.0 00 (0. 1.0 (100 (82 (73 (8 (90 (45-62 04 0.43 (0.436 02 0.24 2 (0.246 0.13 (0.132 0.12 (0.12 (0.13 0.07 (0.077 0. (0 1. (10 (7 ( (9 (45-6 0.4 (0.43 0.2 (0.24 0.1 (0.1 (0.07 (1 (45- (0.4 (0.2 (45 (4