ABSTRACT Currently with a more demanding market, is necessary to produce better and high physiological quality cultivars of soybeans. In front of this new challenge, our objective was to evaluate the physiological quality of 11 soybean cultivars: CD201, SYN1263, SYN1279, BMX, UFLA1, CA115, CD215, CD202, Conquista, Savana, and BRS820 storage for 12 months. Evaluations were conducted through physiological like germination and vigor (accelerated aging and controlled deterioration) and isoenzymes analysis. The seeds were stored under controlled conditions at 10 °C and 10% relative humidity. It was assessed every four months (0, 4, 8, and 12). 200 seeds per treatment were used for each test, divided into 4 replications of 50 seeds. The number of normal plants was evaluated on the fifth and seventh days, expressed as a percentage value. Isoenzyme analysis of MDH, ADH, Esterase, and Catalase was made. The results were interpreted from the presence or absence of bands in the gel. An experimental design in randomized complete blocks, interpreting data using analysis of variance in a factorial scheme 11 x 4 (11 cultivars and 4 times of storage and averages compared by the test Scott-Knott 5% of probability and regression analysis. The statistical program used was Sisvar®. We found that cultivars Savana and Conquista showed low physiological quality, and the cultivars CD 215 and BMX showed high physiological quality during storage. market soybeans challenge 1 CD201 SYN1263 SYN SYN1279 UFLA1 UFLA CA115 CA CD215 CD202 BRS BRS82 accelerated deterioration C humidity 0, 0 (0 8 12. . 12) 20 5 days value MDH ADH Esterase made gel blocks (1 ScottKnott Scott Knott Sisvar Sisvar® 21 CD20 SYN126 SYN127 CA11 CD21 BRS8 ( 2 CD2 SYN12 CA1 SYN1

Resumen (Objetivo). Se evaluó el manglar de Chacarita en el Pacífico Central de Costa Rica, a través de la composición florística y las poblaciones de moluscos asociados de interés comercial, con el fin de proponer actividades de aprovechamiento sustentables en el manglar. (Metodología). Mensualmente y durante un año, se realizaron visitas al área de estudio, con el propósito de establecer una base florística de árboles de mangle y medir el almacenamiento de carbono en el lugar, además, de recolectar moluscos bivalvos para analizar el estado de las poblaciones, con base en las abundancias de organismos hallados y otras características como las longitudes y la masa de estos. En los mangles, se obtuvo el diámetro de la altura de pecho (DAP en cm), la altura (m), el índice de valor de importancia (IVI) y las reservas de carbono (Mg C·ha-1). En cuanto a los moluscos, se examinaron cuatro especies de interés comercial, evaluando la longitud (cm), la masa (g) y el índice gonadosomático. Finalmente, se realizaron valoraciones económicas de los datos obtenidos en el manglar, del turismo e información histórica de pesca para el acopio de Chacarita. (Resultados). Se hallaron cinco especies de mangles, Rhizophora mangle, Rizophora racemosa, Laguncularia racemosa, Avicennia germinans y Pelliciera rhizophorae; P. rhizophorae fue la de mayor índice de valor de importancia. Se obtuvo un almacenamiento de carbono equivalente a 78.49 Mg C·ha-1. Se contabilizaron 357 organismos, de los cuales el 18 % correspondió a Anadara similis, 37.5 % a Anadara tuberculosa, 41.46 % a Leukoma spp. y 3.08 % a Mytella guyanensis. Tomando en cuenta el valor económico de las reservas de carbono y los moluscos bivalvos presentes en los muestreos, se obtuvieron valoraciones económicas de 9812.63 USD·ha-1 y 28 USD·ha-1, respectivamente. (Conclusiones). Las bajas densidades poblacionales de moluscos, especialmente del género Anadara, hacen necesario establecer medidas de gestión que permitan recuperar las poblaciones de estos. La presente investigación propone actividades de aprovechamiento sustentables, como turismo comunitario con fines educativos y culturales.

Resumo (Objetivo). O mangue de Chacarita, no Pacífico Central da Costa Rica, foi avaliado por meio da composição florística e das populações de moluscos associados de interesse comercial, com o objetivo de propor atividades de uso sustentável no mangue. (Metodologia). Foram feitas visitas mensais à área de estudo durante um ano para estabelecer uma base florística de árvores do mangue e medir o armazenamento de carbono no local, bem como para coletar moluscos bivalves para analisar o status das populações, com base na abundância de organismos encontrados e em outras características, como comprimento e massa de tais organismos. Nos manguezais, foram obtidos o diâmetro à altura do peito (DAP em cm), a altura (m), o índice de valor de importância (IVI) e as reservas de carbono (Mg C-ha-1). No que diz respeito aos moluscos, foram examinadas quatro espécies de interesse comercial., avaliando o comprimento (cm), a massa (g) e o índice gonadossomático. Por fim, foram feitas avaliações econômicas de dados de mangue, turismo e informações históricas de pesca para a coleção Chacarita. (Resultados). Foram encontradas cinco espécies de mangue, Rhizophora mangle, Rizophora racemosa, Laguncularia racemosa, Avicennia germinans e Pelliciera rhizophorae; a P. rhizophorae apresentou o índice de valor de importância mais alto. Foi obtido um armazenamento de carbono equivalente a 78.49 Mg C-ha-1. Foram calculados um total de 357 organismos, dos quais 18% correspondeu a Anadara similis, 37.5% Anadara tuberculosa, 41.46% Leukoma spp. e 3.08% Mytella guyanensis. Levando em conta o valor econômico das reservas de carbono e dos moluscos bivalves presentes nas amostras, foram obtidas avaliações econômicas de US$ 9812.63 ha-1 e US$ 28 ha-1, respectivamente. (Conclusões). As baixas densidades populacionais de moluscos, especialmente do gênero Anadara tornam necessário o estabelecimento de medidas de manejo para permitir a recuperação dessas populações. Esta pesquisa propõe atividades de uso sustentável, como o turismo comunitário para fins educacionais e culturais.

Abstract (Objective). The floristic composition and populations of associated molluscs of commercial interest of the Chacarita mangrove forest on the Central Pacific of Costa Rica were evaluated to guide proposals for sustainable exploitation activities in the mangrove forest. (Methodology). Monthly visits to the study area were conducted for one year to establish a floristic baseline of mangrove trees and measure carbon storage. Additionally, bivalve molluscs were collected to analyze the state of their populations based on the abundances of organisms found, as well as other characteristics such as length and mass. Data regarding the mangrove trees included their diameter at breast height (DBH, cm), height (m), importance value index (IVI), and carbon reserves (Mg C·ha-1). Four mollusc species of commercial interest were analyzed, using data on their length (cm), weight (g), and gonodosomatic index. Economic evaluations were also conducted using data obtained from the mangrove forest, as well as tourism and historical fishing data for the collection of Chacarita. (Results). Five species of mangroves were identified: Rhizophora mangle, Rizophora racemosa, Laguncularia racemosa, Avice germinans, and Pelliciera rhizophorae, while P. rhizophorae had the highest importance value index. The study revealed a carbon storage equivalent to 78.49 Mg C·ha-1. A total of 357 organisms were counted, with Anadara similis accounting for 18% of the total count, followed by Anadara tuberculosa (37.5%), Leukoma spp. (41.46%), and Mytella guyanensis (3.08%). When considering the economic value of carbon stocks and the bivalve molluscs obtained from the samples, economic valuations of 9,812.63 USD·ha-1 and 28 USD·ha-1 were determined, respectively. (Conclusions). The low population densities of molluscs, particularly those belonging to the Anadara genus, necessitate the establishment of management measures to facilitate the recovery of these mollusk populations. This research proposes sustainable use activities such as community tourism for educational and cultural purposes.

Abstract: Smart Grids have revolutionized the electricity industry by enabling more efficient control and monitoring of the electricity supply, with a key component being smart meters (SM). These collect information on demand, energy, and harmonic distortion, among others, which must be stored and managed efficiently in a metering data management system (MDMS). The MDMS must ensure that a complete set of data is obtained for use in algorithms to ensure the reliability and quality of the power supply. To address the challenge of management the big data generated by SM, short, medium, and long-term measurement forecasting techniques have been proposed, highlighting the use of artificial intelligence such as Artificial Neural Networks (ANN) and Deep Learning (DL) methods due to their ability to adapt to different input and output variables with various time horizons. In addition, the influence of the diversity of Information and Communication Technologies (ICT) on the update time and data storage in the MDMS is highlighted. In this sense, this work aims to identify which ANN or DL architecture(s) could be more suitable for enterprise, survey, or research applications, demonstrating favorable performance metrics in different scenarios of sampling frequency and typical data update times in Smart Grids. This is relevant due to the need for MDMS to perform multivariate and multi-pass predictions in the short term to complete the information until the information is available or updated.

Resumen: Las Redes Eléctricas Inteligentes de Distribución (REID) han revolucionado la industria eléctrica al permitir un control y monitoreo más eficiente del suministro eléctrico, con un componente clave siendo los medidores inteligentes (SM). Estos recopilan información sobre la demanda, energía, distorsión armónica, entre otros, que deben ser almacenados y gestionados eficientemente en un sistema de gestión de datos de medición (MDMS). El MDMS debe garantizar la obtención de un conjunto completo de datos para su uso en algoritmos que aseguren la confiabilidad y calidad del suministro eléctrico. Para abordar el desafío de gestionar la gran cantidad de datos generados por los SM, se han propuesto diferentes técnicas de predicción de mediciones a corto, mediano y largo plazo, destacando el uso de inteligencia artificial como las Redes Neuronales Artificiales (ANN) y métodos de Aprendizaje Profundo (DL) debido a su capacidad de adaptación a diferentes variables de entrada y salida con diversos horizontes temporales. Además, se destaca la influencia de la diversidad de Tecnologías de la Información y Comunicación (TIC) en el tiempo de actualización y almacenamiento de datos en un MDMS. En este sentido, este trabajo tiene como objetivo identificar qué arquitectura o arquitecturas de ANN o DL podrían ser más adecuadas para aplicaciones en empresas, estudios o investigaciones, demostrando métricas de rendimiento favorables en diferentes escenarios de frecuencia de muestreo y tiempos de actualización de datos típicos en un REID. Esto es relevante debido a la necesidad del MDMS de realizar predicciones multivariables y multipaso a corto plazo para completar la información, hasta que la misma esté disponible o se actualice.

ABSTRACT Grains are hygroscopic materials and, during storage, they are subject to exchanging moisture with the environment according to temperature and relative air humidity, making it important to monitor these factors. Digital temperature and relative air humidity sensors appear as an alternative for monitoring the moisture of grain mass inside silos; they are simple to install and use. Digital sensors present outstanding precision in temperature measurements. The objective in this study was to assess the efficiency and applicability of intergranular relative air humidity sensors linked to digital temperature sensors in the thermometry system of silos storing soybeans. Two samples of soybeans were analyzed in the upper, middle and lower thirds of the silo, for grain mass temperature, intergranular relative air humidity, estimated equilibrium moisture content according to collected data, and moisture content of the sampled grains. Moisture contents obtained from sensor measurements using the hygroscopic equilibrium equation and determined using the oven method were compared. The equilibrium moisture content estimated by the data provided by the sensors did not differ by the Tukey test (p ≤ 0.05) from the moisture content determined by the oven method. Digital temperature and relative air humidity sensors have proven to be efficient, as they contribute to estimating the equilibrium moisture content with satisfactory precision. storage factors use upper silo grains compared p 0.05 005 0 05 efficient 0.0 00 0.

RESUMO Grãos são materiais higroscópicos, e em seu armazenamento estão sujeitos às trocas de vapor de água de acordo com a temperatura e umidade relativa do ar, sendo importante o monitoramento desses fatores. Os sensores digitais de temperatura e umidade relativa do ar surgem como alternativa para monitoramento da temperatura da massa de grãos no interior de silos, apresentando simplicidade de instalação e utilização. Sensores digitais apresentam destacada precisão nas medições de temperatura. O objetivo deste estudo foi verificar a eficiência e aplicabilidade de sensores de umidade relativa do ar intergranular atrelados a sensores digitais de temperatura no sistema de termometria de silos armazenando grãos de soja. Duas amostragens de grãos de soja no terço superior, médio e inferior do silo foram analisadas para a temperatura da massa de grãos, umidade relativa do ar intergranular, teor de água de equilíbrio estimado conforme dados coletados e teor de água dos grãos amostrados. Realizou-se a comparação do teor de água obtido a partir das medições dos sensores usando a equação do equilíbrio higroscópico e pela determinação através do método da estufa. O teor de água de equilíbrio estimado pelos dados fornecidos pelos sensores não diferenciou pelo teste de Tukey (p ≤ 0.05) do teor de água determinado pela estufa. Os sensores digitais de temperatura e umidade relativa do ar demonstraram-se eficientes, visto que contribuem para estimativa do teor de água de equilíbrio com precisão satisfatória. higroscópicos fatores utilização superior amostrados Realizouse Realizou se estufa p 0.05 005 0 05 demonstraramse demonstraram eficientes satisfatória 0.0 00 0.

ABSTRACT This study aims to develop an eco-friendly, recyclable, and cost-effective composite material for use in storage sheds and machine workshops. The new composite consists of stone dust and gravel bricks bonded by recycled polypropylene through heat treatment. The main focus of this study is to determine the effects of varying polymer proportions on the resistance and permeability properties of the studied composite, intending to achieve optimal properties, i.e., high resistance and low permeability. To do this, the 2² factorial arrangement was employed, comprising four treatments along with three central points, each replicated three times. The data was statistically analyzed at a 95% confidence level from an adapted methodology. This involved tests of compression strength, 3-point bending strength, Los Angeles abrasion, and water absorption by immersion, in which three percentages of polymer content (PC) - 15, 25, and 35% - and aggregates (fine sand, medium sand, and coarse sand - FS, MS, and CS) were used to prepare the composite. The developed composite was deemed suitable for use in storage warehouses and machine workshops, as it presented physical and mechanical appropriate characteristics, i.e., a low water absorption rate and high resistance to compression and abrasion, in addition to being an environmentally friendly composite. ecofriendly, ecofriendly eco friendly, eco-friendly recyclable costeffective cost effective workshops treatment ie i e i.e. 2 employed points times 95 methodology strength 3point point 3 abrasion immersion PC (PC 15 25 35 fine FS MS CS characteristics i.e 9 1

RESUMO Este estudo tem como objetivo desenvolver um material compósito ecológico, reciclável e de baixo custo para uso em galpões de armazenamento e oficinas de máquinas. O novo compósito consiste em um bloco de pó de pedra e cascalho unidos por polipropileno reciclado através de tratamento térmico. O foco principal deste estudo é determinar os efeitos de variações nas proporções de polímeros nas propriedades de resistência e permeabilidade do compósito estudado, visando alcançar propriedades ótimas, ou seja, alta resistência e baixa permeabilidade. Para isso, utilizou-se o arranjo fatorial 2², composto por quatro tratamentos e três pontos centrais, cada um replicado três vezes. Os dados foram analisados estatisticamente com nível de confiança de 95% a partir de metodologia adaptada. Isso envolveu testes de resistência à compressão, resistência à flexão em 3 pontos, abrasão Los Angeles e absorção de água por imersão, nos quais três porcentagens de teor de polímero (PC) - 15, 25 e 35% - e agregados (areia fina, areia média, e areia grossa - FS, MS e CS) foram usadas para preparar o compósito. O compósito desenvolvido foi considerado adequado para utilização em armazéns e oficinas de máquinas, pois apresentou características físicas e mecânicas adequadas, ou seja, baixa taxa de absorção de água e alta resistência à compressão e abrasão, além de ser um compósito ecologicamente correto. ecológico máquinas térmico estudado ótimas seja isso utilizouse utilizou se 2² centrais vezes 95 adaptada imersão PC (PC 15 2 35 fina média FS CS adequadas correto 9 1

Abstract The study of the freshwater-saltwater interface zone (FWSWI) is fundamental for the characterization of coastal aquifers and the evolution of freshwater reserves. In this work we study the factors that condition the dynamics of the IADAS in the locality of Pinamar. To carry out this task, a monitoring borehole was drilled on the coastal front, electrical conductivity (EC) profiling was performed, and continuous pressure, temperature and salinity sensors were installed. Information was collected from the tide gauge located in the town of Santa Teresita, while hydrometeorological data were obtained from a station located in the town of Pinamar. A correlation between high tide events and salinity, water level and temperature values were verified. The response times between the rise of the tide and the increase of the salinity are less than 8 hours, while the interval required to reach equilibrium again takes at least 2 days. Temperature variations are influenced by the prevailing climatic conditions at the time of the rising tide. The analysis of the relationship between storage variation and high tide events allows inferring that, when recharge events occur, there is a greater stability due to the increase of the hydraulic load on the IADAS. The study of IADAS behavior is an effective indicator for resource management.

Resumen El estudio de la zona de interfase agua dulce-agua salada (IADAS) resulta fundamental para la caracterización de acuíferos costeros y la evolución de las reservas de agua dulce. En este trabajo se estudian los factores que condicionan la dinámica de la IADAS en la localidad de Pinamar. Para llevar a cabo esta tarea se realizó una perforación de monitoreo sobre el frente costero, se realizaron perfilajes de conductividad eléctrica (CE) y se instalaron sensores continuos presión, temperatura y salinidad. Se recopiló información proveniente del mareógrafo ubicado en la localidad de Santa Teresita mientras que los datos hidrometeorológicos se obtuvieron a partir de estación ubicada en la localidad de Pinamar. Se verifica una correlación entre los eventos de pleamar y los valores de conductividad eléctrica, nivel hidráulico y temperatura. Los tiempos de respuesta entre el ascenso de la marea y el aumento de la salinidad se reconocen menos de 8 horas, en tanto que el intervalo requerido para alcanza nuevamente el equilibrio demora al menos 2 días. Las variaciones de la temperatura se encuentran influenciadas por las condiciones climáticas dominantes al momento del ascenso de la marea. El análisis de la relación entre la variación del almacenamiento y los eventos de pleamar permiten inferir que, cuando se producen eventos de recarga, existe una mayor estabilidad debido al incremento de la carga hidráulica sobre la IADAS. El estudio del comportamiento de la IADAS resulta un indicador eficaz para la gestión de los recursos hídricos en sectores costeros.

Abstract: The dry forest ecosystem is characterized by their rich biodiversity and adaptations to arid conditions. This study focused on determining the composition and structure of the vegetation, examining species interactions, and estimating carbon stored in its aboveground biomass (AGB) using an allometric equation proposed for mixed dry forests. We used 10 plots of 10 x 20 m to record data on trees with a diameter at breast height (DBH) ≥ 5 cm. Taxonomic classification was initially obtained using experts and specialized databases. Ecological importance was assessed through the Importance Value Index (IVI), and species association was determined using the Indicator Value Index (IndVal%). We identified 148 individuals of 21 species, 19 genera, and 12 families in four groups with strong associations, with C. Trischistandra standing out for its high IVI. The Kruskal-Wallis test did not show significant differences in carbon stored between plots, and was estimated a storage potential of 70.47 Mg C ha-1. This research highlights the importance of key species in carbon capture, which is crucial for mitigating climate change. Effective management of these species could have a positive impact on the conservation of the dry forest ecosystem and the fight against global warming. This analysis provides a deep understanding of the structure of this ecosystem.

Resumen: El ecosistema del bosque seco se caracteriza por su rica biodiversidad y adaptaciones a condiciones áridas. Este estudio se centró en determinar la composición y estructura de la vegetación, examinar las interacciones entre las especies y estimar el carbono almacenado en su biomasa aérea (AGB), se usó una ecuación alométrica propuesta para bosques secos mixtos. Utilizamos 10 parcelas de 10 x 20 m para registrar datos sobre árboles con un diámetro a la altura del pecho (DAP) ≥ 5 cm. Inicialmente, la clasificación taxonómica se obtuvo consultando a expertos y bases de datos especializadas. La importancia ecológica se evaluó a través del Índice de Valor de Importancia (IVI) y la asociación de especies se determinó utilizando el Índice de Valor Indicador (IndVal%). Se identificaron 148 individuos de 21 especies, 19 géneros y 12 familias en cuatro grupos con fuertes asociaciones, destacándose C. Trischistandra por su alto IVI. El test de Kruskal-Wallis no mostró diferencias significativas en el carbono almacenado entre las parcelas y se estimó un potencial de almacenamiento de 70.47 Mg C ha-1. Esta investigación resalta la importancia de especies clave en la captura de carbono, lo cual es crucial para mitigar el cambio climático. El manejo efectivo de estas especies podría tener un impacto positivo en la conservación del ecosistema de bosque seco y en la lucha contra el calentamiento global. Este análisis brinda un entendimiento profundo de la estructura de este ecosistema.

Abstract The physical and virtual infrastructure are important aspects to evaluate in organizations, since, if not, there could be deficiencies in operations, non-functional equipment and furniture, poor control of data and electronic resources, poor technological adaptability, to mention a few. Even more so, in an era of increasing remote work, technological dependence and demand for adequate conditions in the work area. The objective is to design and validate an instrument that collects information related to the perception of infrastructure in an organization, under a quantitative, cross-sectional, correlational, non-exploratory and descriptive approach. The methodology was: panel of experts validated the content; second, factor analysis validated the construct, using the KMO test (Kaiser Meyer Olkin) and Bartlett's sphericity; third, the reliability of the instrument was obtained with Cronbach's Alpha coefficient. The result, after applying the pilot test to 58 individuals was: KMO 0.738; Bartlett's sphericity 0.000; Cronbach's Alpha coefficient of 0.942 for the complete instrument. Finally, the findings and conclusions are the increase of four dimensions proposed by ISO to six, with their respective Cronbach's Alpha coefficient, called: information processors (0.882); cybersecurity and telecommunications (0.887); electronic devices, internet service and transportation (0.865); physical and virtual space (0.809); information exchange and storage (0.724); ergonomics and ventilation in the workspace (0.861). The main contribution is an instrument with scientific rigor, relevant for general use in the academic community and any productive sector.

Resumo As infra-estruturas físicas e virtuais são aspectos importantes a avaliar nas organizações, pois, caso não o façam, podem surgir deficiências nas operações, nos equipamentos e no mobiliário não funcional, bem como um mau controlo dos dados e dos recursos electrónicos e uma fraca adaptabilidade tecnológica, entre outros. . Portanto, o objetivo deste trabalho é projetar e validar um instrumento que colete informações relacionadas à percepção de infraestrutura em uma organização. Para isso, utilizou-se uma abordagem quantitativa, transversal, correlacional, não exploratória e descritiva. A metodologia consistiu em um painel de especialistas que validou o conteúdo, bem como uma análise fatorial que validou o construto por meio do teste KMO (Kaiser Meyer Olkin) e esfericidade de Bartlett. Além disso, a confiabilidade do instrumento foi obtida por meio do coeficiente alfa de Cronbach. Após aplicação do teste piloto em 58 indivíduos, o resultado foi o seguinte: KMO 0,738, esfericidade de Bartlett 0,000 e coeficiente alfa de Cronbach de 0,942 para o instrumento completo. Por fim, os resultados permitiram determinar o aumento de quatro para seis dimensões propostas pela ISO com seus respectivos coeficientes alfa de Cronbach, denominados processadores de informação (0,882); cibersegurança e telecomunicações (0,887); aparelhos eletrônicos, serviço de internet e transporte (0,865); espaço físico e virtual (0,809); troca e armazenamento de informações (0,724); ergonomia e ventilação no ambiente de trabalho (0,861). Em resumo, a principal contribuição é um instrumento relevante e com rigor científico para uso geral na comunidade acadêmica e em qualquer setor produtivo.

Resumen La infraestructura física y virtual son aspectos importantes por evaluar en las organizaciones, ya que, en caso de no hacerlo, pueden surgir deficiencias en las operaciones, el equipo y el mobiliario no funcional, así como escaso control de datos y recursos electrónicos y poca adaptabilidad tecnológica, entre otros. Por eso, el objetivo de este trabajo es diseñar y validar un instrumento que recolecte información relacionada con la percepción sobre infraestructura en una organización. Para ello, se empleó un enfoque cuantitativo, transversal, correlacional, no exploratorio y descriptivo. La metodológica consistió en un panel de expertos que validó el contenido, así como en un análisis factorial que validó el constructo usando la prueba KMO (Kaiser Meyer Olkin) y la esfericidad de Bartlett. Además, con el coeficiente alfa de Cronbach se obtuvo la confiabilidad del instrumento. Después de aplicar la prueba piloto a 58 individuos, el resultado fue el siguiente: KMO 0.738, esfericidad de Bartlett 0.000 y coeficiente alfa de Cronbach de 0.942 para el instrumento completo. Finalmente, los hallazgos permitieron determinar el incremento de cuatro a seis dimensiones propuestas por ISO con su respectivo coeficiente alfa de Cronbach, denominadas procesadores de información (0.882); ciberseguridad y telecomunicaciones (0.887); dispositivos electrónicos, servicio de internet y transporte (0.865); espacio físico y virtual (0.809); intercambio y almacenamiento de información (0.724); ergonomía y ventilación en el espacio de trabajo (0.861). En síntesis, la principal contribución es un instrumento pertinente y con rigor científico para el uso general en la comunidad académica y cualquier sector productivo.

Abstract The objective of this research was to identify bacteria present in the microbiota of dentinal carious lesions in primary molars of some Costa Rican pediatric patients. Data were collected from 15 children aged between 4 and 8 years old who attended the Pediatric Dentistry Clinic at the Faculty of Dentistry from the University of Costa Rica (UCR). The inclusion criteria were: infants between 4 and 8 years old who presented cavitated carious lesions in primary teeth, who were actively attended by students at the Faculty of Dentistry from the UCR, and whose parents or legal guardians signed the informed consent to participate in this research. Samples were taken using a sterile spoon, placed in storage vials, and subjected to various conventional and molecular microbial identification techniques, such as Gram stain identification, catalase tests, oxidase, TSI, API 20E, API STAPH, and VITEK 2. Of the 60 bacterial strains subjected to Gram staining, the following was obtained: 28 Gram-positive bacteria and 32 Gram-negative bacteria. The main isolated organisms were species of Staphylococcus epidermidis, Pasteurella pneumotropica/Mannheimia haemolytica, Pantoea spp, and Streptococcus mutans.

Resumen El objetivo de esta investigación fue identificar las bacterias presentes en la microbiota de lesiones cariosas dentinales en molares primarias de pacientes pediátricos costarricenses. Las muestras fueron recolectadas de 15 niños entre los 4 y 8 años que fueron atendidos en la Clínica de Odontopediatría en la Facultad de Odontología de la Universidad de Costa Rica (UCR). Los criterios de inclusión fueron: pacientes entre los 4 y los 8 años de edad que presentaran lesiones cariosas cavitadas en dientes primarios, que se encuentraran activos para su atención por estudiantes de la Facultad de Odontología de la UCR, y que los padres o encargados legales firmaran el consentimiento informado para participar en esta investigación. Las muestras se tomaron utilizando una cuchareta estéril, colocándolas en viales de almacenamiento y fueron sometidas a diversas técnicas de identificación microbiana convencionales y moleculares tales como: identificación por Tinción de Gram, pruebas catalasa, oxidasa, TSI, API 20E, API STAPH y VITEK. De las 60 cepas bacterianas sometidas a tinción de Gram se obtuvo: 28 bacterias Gram Positivas y 32 bacterias Gram Negativas. Los principales organismos aislados fueron: especies de Staphylococcus epidermidis, Pasteurella pneumotropica/ Mannheimia haemolytica, Pantoea spp y Streptococcus mutans.

Abstract Urban trees play an important role within cities due to the environmental services they provide and their contribution to mitigating the current climate crisis. In this context, the objective of this study was to evaluate the tree vegetation of five public parks in the Texcoco de Mora municipality in order to estimate the environmental services they provide. A census was carried out per park and the data were processed through the i-Tree® Eco v6 software. 1 325 individuals from 23 families and 37 genera were recorded with a richness of 55 species in the five parks, an average density of 157 trees ha-1 and a total of 242.6 megagrams of carbon storage with a carbon capture of 10.5 Mg year-1. The avoided rain runoff was 481 m3 year-1 and the value represented by these environmental services is MXN $894 514.93 for carbon storage, MXN $38 650 annually for carbon capture, and MXN $481.93 for runoff. The replacement value of the trees reaches MXN $24 408 534. A tree cover of 30 485 m2 was estimated, which represents a factor of 0.86 m2 inhabitant-1. It is concluded that Texcoco is below the recommended factor of trees per inhabitant proposed by the UN. It is necessary to study the green areas, medians, alignment trees and community parks, to achieve a more complete panorama of the municipality.

Resumen El arbolado urbano desempeña un papel importante dentro de las ciudades por los servicios ambientales que provee y por su contribución a la mitigación de la actual crisis climática. En ese contexto, el objetivo del presente estudio consistió en evaluar la vegetación arbórea de cinco parques públicos del municipio Texcoco de Mora a fin de estimar los servicios ambientales que proporcionan. Se realizó un censo por parque y se procesaron los datos a través del software i-Tree ® Eco V6. Se registraron 1 325 individuos de 23 familias y 37 géneros con una riqueza de 55 especies en los cinco parques, una densidad promedio de 157 árboles ha-1 y un total de 242.6 megagramos de almacenamiento de carbono con una captura de 10.5 Mg año-1. El escurrimiento de lluvia evitado fue de 481 m3 año-1 y el valor que representan estos servicios ambientales en pesos es de MXN $894 514.93 para el almacenamiento de carbono, MXN $38 650 anuales para la captura del mismo y MXN $481.93 para el escurrimiento. El valor de sustitución del arbolado es de MXN $24 408 534.00. Se estimó una cobertura arbórea de 30 485 m2, lo cual representa un factor de 0.86 m2 habitante-1. Se concluye que Texcoco está por debajo del factor recomendado de arbolado por habitante propuesto por la ONU. Es necesario estudiar las áreas verdes, camellones, árboles de alineamiento y parques comunitarios para tener un panorama del municipio más completo.

Abstract Objectives Narrative review evaluating food contamination by endocrine disruptors present in food packaging. Data source The terms “endocrine disruptors” and “food packaging” were used in combination in the PubMed, MEDLINE and SciELO databases, evaluating studies, in humans, published in Portuguese, English, French and Spanish between 1990 and 2023. Data synthesis Packaging, especially those made from plastic or recycled material, is an important source of food contamination by endocrine disruptors. Bisphenols and phthalates are the endocrine disruptors most frequently associated with food contamination from packaging. However, many unknown substances and even those legally authorized can cause harm to health when exposure is prolonged or when substances with additive effects are mixed. Furthermore, the discarding of packaging can cause contamination to continue into the environment. Conclusion Although packaging materials are essential for the transport and storage of food, many of them are associated with chemical contamination. As it is not possible to exclude them from our routine, it is important to develop research aimed at identifying the endocrine disruptors present in them, including the effects of chronic exposure; and that regulatory agencies and industry come together to reduce or prevent this risk. Additionally, consumers must be instructed on how to purchase products, handle them and prepare them to reduce the migration of chemical substances into food. PubMed databases studies humans Portuguese English 199 2023 Packaging material However mixed Furthermore environment routine risk Additionally products 19 202 1 20 2

ABSTRACT Introduction: healthcare workers, especially nurses, laboratory workers and paramedics have a higher risk of exposure to needle-stick injuries, which is sometimes due to inadequate handling and hospital waste storage. Objective: to describe causes of needle-stick injury and preventive measures taken by healthcare workers. Methods: a cross-sectional study was conducted using an online survey; 74 healthcare workers from the Almanzor Aguinaga Asenjo National Hospital and the Lambayeque Regional Hospital participated; data are presented as descriptive statistics; InfoStat 2019 was used for the statistical analysis. Results: the mean age of the respondents was 28.9 years (range 17-48); the majority were female (56.8%), and 43.2%, male. The 56.8% from the total number of respondents (42) had ever had a needle-stick injury, while 43.2% had not received injuries. The severity of the needle stick was mild in 51.4% of the cases, 6.8% was moderate and 41.9% had no injury. Conclusions: needle recapping, venous blood withdrawal and patients' sudden movement during an injection or a suture were the main causes of needle stick found in this study. A continuous training, the use of personal protective equipment, and following the proper disposal guidelines for sharp objects would help health workers to prevent this type of injury, and thus to avoid the transmission of infections through percutaneous blood.

RESUMEN Introducción: Los trabajadores de salud, especialmente enfermeras y enfermeros, laboratoristas y paramédicos tienen mayor riesgo de exposición a lesiones por pinchazo de aguja, en ocasiones, por un inadecuado manejo y almacenamiento de desechos hospitalarios. Objetivo: Describir las causas de lesión por pinchazo de aguja y las medidas de prevención tomadas por los trabajadores de salud. Métodos: Se realizó un estudio de corte transversal utilizando una encuesta en línea; participaron 74 trabajadores de salud del Hospital Nacional Almanzor Aguinaga Asenjo y el Hospital Regional Lambayeque; los datos están presentados como estadística descriptiva; para el análisis estadístico se utilizó InfoStat 2019. Resultados: La edad media de los encuestados fue de 28,9 años (rango de 17-48); la mayoría fueron mujeres (56,8 %), y el 43,2 %, varones. Del total de encuestados (42, 56,8 %), alguna vez tuvo una lesión por pinchazo de aguja, mientras, el 43,2 % no ha recibido lesiones. La severidad del pinchazo de aguja fue leve en 51,4 % de los casos, 6,8 % fue moderada y 41,9 % no tuvo lesión. Conclusiones: Las principales modalidades causantes de pinchazo de aguja encontradas en este estudio fueron: reencapuchar la aguja, extracción de sangre venosa, el movimiento repentino del paciente durante una inyección o una sutura. Una capacitación continua, el uso de equipos de protección personal y seguir las pautas de eliminación adecuada de los objetos cortopunzantes, ayudarían a prevenir este tipo de lesiones en trabajadores de salud y por ende, evitar la transmisión de infecciones a través de sangre percutánea.

ABSTRACT Soil is a medium that stores and transfers air, water, nutrients and heat to microorganisms and plants. Its water storage capacity is studied by analysing water retention curves (WRCs), which establish the relationship between soil water content and the force with which water is retained. The objective of this work was to evaluate the capacity of functional geostatistics in predicting the spatial distribution of water retention curves in two types of soils. Experimental areas with two types of soil, Andisol and Oxisol, were selected, and a regular grid of 75 sites was established, from which water retention curves were obtained at two depths. The curves were subjected to geostatistical functional analysis (GF), and the applicability of this method was evaluated by obtaining the usable water table (LAA) and comparing the results with experimental data obtained using traditional methods. Based on cross-validation, it was verified that GF produced a better fit for the Andisol since the coefficient of determination between the LAA values for the measured data and predictions was high, with an R2 of 99%; however, the proposed methodology was also reliable for the Oxisol, since an R2 of 94% was obtained at the two depths studied. air plants WRCs, WRCs , (WRCs) retained soils Oxisol selected 7 established GF, (GF) (LAA methods crossvalidation, crossvalidation cross validation, validation cross-validation high R 99% 99 however 94 (WRCs (GF 9

RESUMO O solo é um sistema que armazena e transfere ar, água, nutrientes e calor para microrganismos e plantas. Sua capacidade de armazenamento de água é estudada analisando a curva de retenção de água (WRC), que estabelece a relação entre a umidade do solo e a força com que ela é retida. O objetivo deste trabalho foi avaliar a capacidade preditiva da geoestatística funcional na distribuição espacial das curvas de retenção de água em dois tipos de solos. Foram selecionadas duas áreas experimentais, um Andisol e um Oxisol, nas quais foi estabelecida uma grade regular com 75 pontos, das quais foram obtidas curvas de retenção de água em duas profundidades. As curvas foram submetidas à análise geoestatística funcional (GF), sua aplicabilidade foi avaliada obtendo a tabela de água utilizável (LAA) e sua posterior análise espacial por métodos tradicionais nos quais os resultados foram comparados com os dados experimentais. Com base na validação cruzada, verificou-se que o uso de GF tem um melhor ajuste para o Andossolo, uma vez que o coeficiente de determinação entre os valores de LAA obtidos com os dados observados e aqueles obtidos com as previsões foi alto, com R2 de 99%; no entanto, para o Latossolo, a metodologia proposta também é confiável, uma vez que foi obtido um R2 de 94% nas duas profundidades estudadas. ar plantas WRC, WRC , (WRC) retida solos experimentais Oxisol 7 pontos GF, (GF) (LAA cruzada verificouse verificou se Andossolo alto R 99% 99 entanto Latossolo confiável 94 estudadas (WRC (GF 9

INTRODUCTION: Contaminated disinfectants have been occasional vehicles of healthcare associated infections. AIMS AND OBJECTIVES: To determine the presence and level of bacterial contamination of disinfectants used to decontaminate suction devices and to assess the extent to which students comply with infection control practices. DESIGN: A two-part cross-sectional descriptive study consisting of microbiological testing of disinfectants and a questionnaire-based observation of students. METHODS: Unannounced observation of students disinfecting suction devices were recorded using a questionnaire. The process involved collecting a prepared disinfectant from a storage bin using a kitchen measuring jug. Specimens of disinfectants and swabs of jugs were collected for aerobic culture. Data pertaining to compliance with infection control practices was gathered. RESULTS: Only 33.6% of the students were observed unannounced. An overwhelming majority (84.9%) of students disinfected suction devices; 52% cleaned and disinfected the external surface of suction hoses and the spittoon bowl; 18.6% allowed the disinfectant to remain in the system long enough, and 14% advised their patients not to close their lips around the suction device. The majority of disinfectant samples (56.3%) as well jugs (55.6%) were contaminated with bacteria. CONCLUSION: Bacterial contamination of disinfectants was common in addition to poor compliance with infection control practices.

ABSTRACT Objective: The aim of this study was to identify critical aspects of the Cold Chain in the immunization process in Peru. Methodology: A descriptive study was conducted, analyzing data from the Ministry of Economy and Finance (MEF) and the Ministry of Health (MINSA) for the years 2020-2021, as well as administrative records from the MINSA's Regional Health Strategies for immunizations in 2020. Technical aspects established in the Health Technical Standard for Cold Chain Management, such as obsolescence, allocation, functionality, and storage capacity were taken into account. Results: In the year 2020, at the national level, 61.8% of the cold chain equipment showed obsolescence, with some regions exceeding 75%, with Lima’s metropolitan region being the most affected at 88%. Concerning equipment allocation, 9% of the first-level health facilities lacked refrigeration equipment, with Loreto having the highest deficit (46%), followed by Huancavelica with a 21% gap. The overall equipment functionality nationwide was 84%, meaning that 16% of health facilities experienced technical failures, affecting vaccine’s storage capacity and posing risks to their safety and immunogenicity. Significant gaps were identified when considering quarterly or monthly storage for COVID-19 vaccines or other health emergencies. Conclusions: This study highlights potential risks in the operability and storage capacity of the national immunization program's vaccines in Peru during contingencies such as the COVID-19 pandemic or other health emergencies.

RESUMEN Objetivo: Identificar aspectos críticos de la Cadena de Frío en el Perú Metodología: Estudio descriptivo. Se analizaron datos del Ministerio de Economía y Finanzas (MEF) y del Ministerio de Salud (MINSA) de los años 2020-2021, así como los registros administrativos de las Estrategias Sanitarias Regionales de inmunizaciones del MINSA en 2020. Se consideraron aspectos técnicos de la Norma Técnica de Manejo de cadena de frío, como es obsolescencia, dotación, funcionalidad y capacidad de almacenamiento. Resultados: En el año 2020, en términos de obsolescencia el 61.8% de los equipos de cadena de frío a nivel nacional presentaban obsolescencia, siendo regiones claves como Lima Metropolitana (capital del país) la más afectada con un 88%. En cuanto a la dotación de equipos, el 9% de los establecimientos de salud del primer nivel carecen de equipos de refrigeración, siendo Loreto la región con mayor déficit 46%, seguida de Huancavelica con un 21% de brecha. En términos de funcionamiento, se registra que el 84% de los equipos a nivel nacional funcionan, y el 16% reportan fallas técnicas, lo cual representa alto riesgo en la seguridad y potencia inmunogénica de las vacunas a prever. En términos de capacidad, al considerar el almacenamiento trimestral o mensual para las vacunas contra la COVID-19 u otras emergencias sanitarias se identificaron brechas significativas. Conclusiones: Existen riesgos en la operatividad, suministro y capacidad de almacenamiento de las vacunas del esquema nacional de inmunizaciones de Perú incluso ante emergencias sanitarias.