RESUMO Kalanchoe daigremontiana é uma espécie ornamental de propagação obrigatoriamente assexuada. Entretanto, nenhum estudo in vitro foi realizado para avaliar a relação entre mudanças no ambiente de cultivo e variações genéticas de K. daigremontiana. Portanto, o objetivo deste estudo foi estabelecer a melhor concentração de sais no meio Murashige & Skoog (MS) e a concentração de sacarose no meio de cultura, além da qualidade da luz, no desenvolvimento de plântulas de explantes foliares de K. daigremontiana. Além disso, realizamos uma avaliação genética das plântulas resultantes para estudar alterações fenotípicas que poderiam ser atribuídas à variação somaclonal utilizando marcadores da Amplificação Aleatória de DNA polimórfico (RAPD). Para tanto, explantes foliares de K. daigremontiana foram desinfestados e inoculados em diferentes concentrações de sais do meio MS (25, 50 ou 100%), sem sacarose. As culturas foram transferidas para sala de crescimento e submetidas a três condições de luz (branca, azul e vermelha) com fotoperíodo de 16 horas, mais a ausência de luz, sob temperatura de 27 ± 1 °C. Os explantes também foram inoculados em 25% das concentrações de sais do meio MS, suplementado com diferentes concentrações de sacarose (controle - sem sacarose; 1,5; 3,0; 4,5 e 6,0%). Os explantes foliares de K. daigremontiana desenvolveram-se melhor sob luz branca e em qualquer uma das concentrações de sal do meio MS. Meio de cultura isento de sacarose pode ser utilizado e nenhuma variação no perfil genético das plântulas foi observada em comparação com as plantas-mãe nas condições de cultura testadas. assexuada Entretanto K Portanto (MS disso RAPD. RAPD . (RAPD) tanto 25, 25 (25 5 100%, 100 100% , 100%) branca, (branca vermelha horas 2 C °C controle 1,5 15 3,0 30 3 0 45 4 4, 6,0%. 60 6,0% 6 6,0%) desenvolveramse desenvolveram se plantasmãe plantas mãe testadas (RAPD (2 10 1, 3, 6,0 ( 6,

ABSTRACT Kalanchoe daigremontiana is an ornamental species propagated exclusively asexually. However, no in vitro studies have been conducted to assess the relationship between changes in the culture environment and genetic variations of K. daigremontiana. Therefore, this study aimed to determine the optimal concentration of salts in the Murashige & Skoog (MS) medium and the optimal concentration of sucrose in the culture medium, in addition to the quality of light, in the development of in vitro plantlets from leaf explants of K. daigremontiana. Additionally, a genetic evaluation of the resulting plantlets was conducted to study phenotypic changes that could be attributed to somaclonal variation using Random Amplified Polymorphic DNA (RAPD) markers. For this purpose, leaf explants of K. daigremontiana were disinfected and inoculated in different salt concentrations (25, 50, and 100%) of MS medium without sucrose. The cultures were transferred to a growth chamber and subjected to three light conditions (white, blue, and red) with a 16-hour photoperiod, plus the absence of light, at a temperature of 27 ± 1 °C. Explants were also inoculated in 50% salt concentrations of MS medium supplemented with various concentrations of sucrose (control - without sucrose, 1.5, 3.0, 4.5, and 6.0%). K. daigremontiana leaf explants showed optimal development under white light and in any MS medium salt concentrations. A sucrose-free culture medium was viable, and no genetic variation was observed in the plantlets compared to the parent plants under the tested conditions. asexually However K Therefore (MS Additionally RAPD (RAPD markers purpose 25, 25 (25 50 100% 100 white, (white blue red 16hour hour 16 photoperiod 2 C °C control 15 5 1.5 30 3 0 3.0 45 4 4.5 6.0%. 60 6.0% . 6 6.0%) sucrosefree free viable (2 10 1. 3. 4. 6.0 ( 6.

Resumo Ainfluência do comportamento dos usuários em edificações é amplamente discutida como uma das causas para discrepâncias entre consumos energéticos esperados, durante a fase de projeto, e consumos durante a operação. Conhecer os principais motivadores para ajustes nos sistemas de edificações é imprescindível para aprimorar a modelagem desses comportamentos. O objetivo deste estudo é avaliar os principais agentes motivadores para o ajuste das janelas, luminárias e aparelhos de ar-condicionado em escritórios. Dois escritórios foram monitorados por meio de sensores que coletaram dados de qualidade do ambiente interno e comportamento de usuário. Foram consideradas influentes para a interação com sistemas de janelas a pluviometria e as temperaturas do ar externo e interno. O aumento das temperaturas interna e externa apresentou ainda mais influência na interaçãocom aparelhos de ar-condicionado. Duas foram as principais conclusões: há influência do layout dos escritórios no compartilhamento e controle das janelas e eficiência de estratégias passivas, como o uso de ventilação natural e cruzada; e há influência do aumento da temperatura interna em comparação à externa na previsibilidade de abertura de janelas e acionamento de aparelhos de ar-condicionado. esperados projeto operação comportamentos arcondicionado condicionado usuário arcondicionado. condicionado. conclusões passivas cruzada

Abstract The influence of occupant behaviour in buildings is widely discussed as one of the causes for the gap between energy use estimated via computer simulation and during building operation. Knowing the main drivers for adjustments in building systems is essential to guide the development of mathematical models for such behaviours. The aim of this study is to evaluate the main motivating agents for adjusting windows, luminaires, and air-conditioning in offices. Two offices were monitored using sensors that collected data from the internal environment and user interaction with such systems. Rainfall and external and internal air temperatures were considered influential for the interaction with window systems. The increase in internal and external temperatures had even more influence on interactions with air-conditioners. The main conclusions were: there is influence of the layout of the offices in the sharing and control of windows and efficiency of passive strategies, such as the use of natural and cross ventilation; and there is an influence of the increase in internal temperature compared to external temperature in the predictability of opening windows and turning air-conditioning devices on. operation behaviours luminaires airconditioning conditioning airconditioners. airconditioners conditioners. conditioners air-conditioners strategies ventilation

Abstract Since 2010, there has been an approximate annual increase of 1% in CO2 emissions due to buildings. The reduction of energy consumption and consequently the mitigation of Greenhouse Gases are global goals due to the global issue of climate change. A key step in achieving these goals is to improve the energy performance of buildings, thereby reducing energy consumption and emissions generated by the built environment. In this regard, the present research focuses on identifying and estimating strategies to reduce energy consumption in buildings known as Community Police Units (UPCs) located in the city of Guayaquil, which corresponds to a very hot and humid climate zone. The study consists of statistical analysis of the historical energy performance of 43 buildings sharing the same architectural design. Additionally, the selection of a representative building was made for energy use monitoring and energy simulation. The results revealed a potential saving of 16 000 kWh per year by reducing the control temperature of the air conditioning system by 1°C and adjusting the on/off schedule. This is equivalent to 1 150 USD and 5 metric tons of CO2 emissions.

Resumen: Desde el 2010 existe un incremento aproximado del 1% anual respecto a las emisiones de CO2 a causa de los edificios. La disminución del consumo energético y consecuentemente la mitigación de los Gases de Efecto Invernadero son metas a nivel mundial debido a la problemática global del cambio climático. Un paso clave para alcanzar estas metas es mejorar el desempeño energético de las edificaciones y de esta manera reducir la energía consumida y las emisiones generadas por el parque edificado. En este sentido, la presente investigación se centra en identificación y estimación de estrategias para reducir el consumo de energía de los edificios llamados Unidades de Policía Comunitaria (UPC) localizadas en la ciudad de Guayaquil, y que corresponde a una zona climática húmeda muy calurosa. El estudio está compuesto por el análisis estadístico del rendimiento energético histórico de 43 edificios, que comparten el mismo diseño arquitectónico. Además, se realizó la selección de un edificio representativo para el monitoreo del uso de energía y simulación energética. Los resultados evidenciaron un potencial de ahorro de 16 000 kWh al año, al disminuir 1°C la temperatura de control del sistema de climatización y modificando el horario de encendido y apagado. Esto es equivalente a 1 150 USD y 5 toneladas equivalentes de CO2.

RESUMEN Este artículo presenta los resultados de una investigación educativa cuyo propósito fue comprender la influencia de las prácticas educativas en el proceso autorregulatorio de los estudiantes en Colombia. El enfoque metodológico fue de tipo cualitativo con un alcance descriptivo que buscó detalles sobre el hacer docente y el aprendizaje de aula. La muestra se compone de 137 participantes distribuidos en 90 estudiantes, 24 docentes y 23 padres de familia a quienes se les aplicaron una ficha de caracterización, una entrevista y un grupo focal a lo largo de 9 zonas del país: Garzón (Huila), Fortul (Arauca), Bucaramanga (Santander), Bogotá, Tenjo, Guayabal de Siquima (Cundinamarca), Urabá, Medellín, Rionegro (Antioquia), Ibagué (Tolima), Granada (Meta), Santiago de Cali (Valle del Cauca) y Almaguer (Cauca). Para el análisis de la información se contó con una matriz de triangulación y su posterior ejercicio interpretativo. Los resultados muestran que la práctica docente fomenta en los estudiantes su autonomía y su autocontrol a la hora de aprender, mientras que se ve la necesidad de brindar formación sobre las pautas de monitoreo parental. En ese sentido, se reconoce que la educación en contexto a partir de los juegos, la creatividad y el trabajo en grupo incentivan las habilidades de autorregulación del aprendizaje como la metacognición, el monitoreo, la delegación de tareas, la planeación y proyección de las metas a alcanzar.

ABSTRACT This article shows the results of an educational research whose purpose was to understand the influence of educational practices on the self-regulatory process of students in Colombia. The methodological approach was qualitative with a descriptive scope that sought details about the teaching process and classroom learning. The sample is made up of 137 participants distributed among 90 students, 24 teachers and 23 parents to whom a characterization sheet, an interview and a focus group are applied throughout 9 areas of the country: Garzón (Huila), Fortul. (Arauca), Bucaramanga (Santander), Bogotá, Tenjo, Guayabal de Siquima (Cundinamarca), Urabá, Medellín, Rionegro (Antioquia), Ibagué (Tolima), Granada (Meta), Santiago de Cali (Valle del Cauca) and Almaguer (Cauca). For the analysis of the information, a triangulation matrix was used and its subsequent interpretative exercise. The results show that teaching practice encourages students' autonomy and self-control when learning, while there is a need to provide training on parental monitoring guidelines. In this sense, it is recognized that education in context based on games, creativity and group work encourages self-regulation learning skills such as metacognition, monitoring, delegation of tasks, planning and projection of goals to reach.

Resumen: Este estudio buscó evaluar los aportes de un entrenamiento parental híbrido realizado con ocho madres de niños preescolares con trastorno del espectro autista basado en la ciencia del análisis aplicado de la conducta para la reducción del estrés parental. Se llevó a cabo la construcción e implementación de un entrenamiento parental híbrido. Se dividió la muestra en grupo de tratamiento y control, y se realizaron comparaciones del nivel de estrés en el período pre/postintervención, así como el análisis de la práctica directa con el niño en un cara a cara con el grupo de tratamiento. Los resultados obtenidos demuestran que las madres del grupo de tratamiento reaccionaron positivamente a los videos y materiales presentados; sin embargo, no se observaron diferencias estadísticas significativas que sugieran una reducción en el nivel de estrés entre los dos grupos. Las limitaciones del estudio fueron el pequeño tamaño de la muestra y el hecho de que los niños de ambos grupos continuaron recibiendo tratamiento de terapia intensiva durante la implementación de la capacitación de los padres.

Resumo: Este estudo buscou avaliar as contribuições de um treinamento parental híbrido realizado com oito mães de crianças pré-escolares com transtorno do espectro do autismo baseado na ciência da análise do comportamento aplicada para a redução do estresse parental. Foi realizado a construção e implementação do treinamento parental híbrido. A amostra foi dividida em grupo tratamento e controle. Em termos de análise de dados, foram feitas comparações do nível de estresse no período pré/pós-intervenção, assim como a análise de uma prática direta com a criança de forma presencial com o grupo tratamento. Os resultados obtidos demonstraram que as mães do grupo tratamento reagiram positivamente com as vídeos e materiais apresentados. No entanto, não foi observado diferenças estatísticas significativas para sugerir a redução do nível de estresse entre os dois grupos. As limitações do estudo foram o tamanho reduzido da amostra e o fato das crianças de ambos os grupos continuarem recebendo o tratamento em terapia intensiva durante a aplicação do treinamento parental.

Abstract: This study sought to evaluate the contributions of a hybrid parental training carried out with eight mothers of preschool children with autism spectrum disorder, based on the science of applied behavioral analysis for reducing parental stress. The hybrid parental training was constructed and implemented by the researchers. The sample was divided into two groups: a Treatment Group and a Control Group. In the data analysis, comparisons were made of the levels of stress pre- and post-intervention and for the treatment group, direct practice with the child in a face-to-face environment was also analyzed. The results showed that the mothers in the treatment group reacted positively to the video and materials presented; however, no significant statistical differences were found that would suggest a reduction in stress levels between the two groups as a result of the training given. The limitations of the study were its small sample size and the fact that the children in both groups continued to receive intensive therapy treatment during the application of the parental training.

Abstract Introduction. Pesticides are used to control pests and diseases in agricultural activities. Improper management of these products poses a potential risk to human health and the environment. Objective. To evaluate the (eco)toxicological pressure exerted by synthetic pesticides on tobacco crops (Nicotiana tabacum) in the province of Sancti Spíritus, Cuba. Materials and methods. A database from the Provincial Directorate of Plant Health for the period 2016-2019 was used, where the pesticides assigned to the province for this crop were compared with those reported by interviewed farmers. The dual indicator POCER was used to obtain the results, determining the pressure exerted by different pesticides and their harmful reference on humans and the environment. Results. The trend in pesticide consumption (summarized in tables and graphs) showed 50 % decrease, corresponding to the country’s crop protection policy. Farmers mentioned the use of authorized and unauthorized active ingredients in the conducted surveys, and sixteen high-risk active ingredients were detected in the analyzed samples. Evaluating the POCER results and analyzed samples, a significant (eco)toxicological pressure was observed on both the environment and human health, exerted by a group of high-toxicity active ingredients used in tobacco cultivation in the province. Conclusion. Using the dual indicator POCER provides an evaluation of the (eco)toxicological pressure exerted by synthetic pesticides used in the tobacco cultivation (Nicotiana tabacum) in the province of Sancti Spíritus, Cuba, for the study period.

Resumen Introducción. Los plaguicidas son utilizados para controlar las plagas y enfermedades en las actividades agrícolas. El manejo inadecuado de estos productos constituye un riesgo potencial para la salud humana y el medio ambiente. Objetivo. Evaluar la presión toxicológica (eco) ejercida por los plaguicidas sintéticos en los cultivos de tabaco (Nicotiana tabacum) en la provincia de Sancti Spíritus, Cuba. Materiales y métodos. Se utilizó una base de datos de la Dirección Provincial de Sanidad Vegetal para el período 2016-2019, donde se tomaron los plaguicidas asignados a la provincia para este cultivo y se compararon con los declarados por los agricultores entrevistados. Para la obtención de los resultados se utilizó el indicador dual POCER, que determinó la presión ejercida por los diferentes plaguicidas y su referencia nociva sobre el ser humano y el medio ambiente. Resultados. La tendencia del consumo de plaguicidas (resumida en tablas y gráficos) mostró una disminución del 50 %, que corresponde a la política de protección de los cultivos del país. Los agricultores mencionaron el uso de ingredientes activos autorizados y no autorizados en las encuestas realizadas, y en las muestras analizadas se detectaron dieciséis ingredientes activos de alto riesgo. Al evaluar los resultados de POCER y muestras analizadas se observó que existe una gran presión (eco)toxicológica tanto para el medio ambiente como para la salud humana ejercida por un grupo de ingredientes activos de alta toxicidad utilizados en el cultivo de tabaco de la provincia. Conclusión. Con la utilización del indicador dual POCER, se obtiene una evaluación de la presión (eco)toxicológica ejercida por los plaguicidas sintéticos utilizados en el cultivo del tabaco (Nicotiana tabacum) en la provincia de Sancti Spíritus, Cuba para el período de estudio.

ABSTRACT Plant mucilage constitute a viable alternative to reduce dependence on inorganic products that are unfriendly to the environment, which the scientific community must promote to develop new products. The aim was to evaluate mucilage obtained from Triumfetta lappula, as a thickener in a shampoo formula. Prior to the extraction of the mucilage by hydration, phytochemical screening was carried out, which identified the presence of secondary metabolites through rapid coloring and precipitation reactions; and the viscosity of the mucilage (thickening material) was determined using a digital rheometer. For the shampoo, an experimental design of three factors at two levels was used, assessed with the viscosity response variable, and tabulated with the Statgraphics Centurion XV statistical software and foam control by test tube test. The mucilage contains tannins, alkaloids, and triterpenes important as antimicrobials; Its ability to absorb water forms a thick material of 1170 mPa.s, which as an ingredient in the shampoo allowed an average value of apparent viscosity of 1176 mPa.s and an average value of viscosity in the best treatment (T2) of 1530 mPa.s at pH 6,8; values that are within the viscosity range for commercial shampoos. Triumfetta lappula mucilage with the presence of alkaloids, tannins and triterpenes used as a natural thickener, emollient, moisturizing and antibacterial in a shampoo formula, offers satisfactory results that enable new lines of research for formulations of personal care products with important application projections. immediate.

RESUMEN Los mucílagos vegetales constituyen una alternativa viable para reducir la dependencia de productos inorgánicos poco amigables con el medioambiente, que la comunidad científica debe impulsar para desarrollar nuevos productos. El objetivo fue valorar mucílago obtenido de Triumfetta lappula., como espesante en una fórmula de champú. Previo a la extracción del mucílago por hidratación, se realizó el tamizaje fitoquímico que identificó la presencia de metabolitos secundarios por reacciones rápidas de coloración y precipitación; y, la viscosidad del mucílago (material espesante) se determinó utilizando un reómetro digital. Para el champú, se utilizó un diseño experimental de tres factores a dos niveles, valorados con la variable respuesta viscosidad y tabulada con el software estadístico Statgraphics Centurion XV y el control de espuma mediante prueba de la probeta. El mucílago contiene taninos, alcaloides y triterpenos importantes como antimicrobianos; su capacidad para absorber agua forma un material espeso de 1170 mPa.s, que como ingrediente en el champú permitió un valor promedio de viscosidad aparente de 1176 mPa.s y un valor promedio de viscosidad en el mejor tratamiento (T2) de 1530 mPa.s a pH de 6,8; valores que están dentro del rango de viscosidad para champús comerciales. El mucílago de Triumfetta lappula con presencia de alcaloides, taninos y triterpenos utilizado como espesante natural, emoliente, hidratante y antibacterial en una fórmula de champú, ofrece resultados satisfactorios que viabilizan nuevas líneas de investigación para formulaciones de productos de aseo personal con importante proyección de aplicación inmediata.

Resumen Introducción: Las competencias socioemocionales en la comunicación cara a cara son herramientas esenciales para el ajuste personal y social desde temprana edad. La comunicación en línea ha adquirido igual importancia, especialmente durante la adolescencia. Si bien se reconoce que sus características requieren habilidades específicas, hay escasez de estudios que analicen la relación existente entre las habilidades socioemocionales generalmente adquiridas y aquellas necesarias para la comunicación en línea. Por tanto, esta investigación tiene el objetivo de analizar cómo las habilidades socioemocionales fuera de línea predicen las competencias en línea entre chicas y chicos de manera diferenciada. Método: Este estudio incluyó a 478 estudiantes de educación secundaria con edades entre los 10 y 14 años. Este es un estudio transversal que utilizó el Path Analysis diferenciado por sexo, con muestreo intencional y no probabilístico. Para evaluar las competencias socioemocionales, se utilizó el instrumento ESCQ-21 para las competencias socioemocionales generales el cuestionario e-COM para cuantificar las competencias socioemocionales en línea. Resultados: El modelo explicativo indica que las competencias socioemocionales se transfieren parcialmente al entorno en línea en ambos sexos, especialmente la Percepción y comprensión emocional. Además, se sugiere que el E-autocontrol de la impulsividad y la E-autonomía emocional requieren un desarrollo específico en ese entorno. Conclusiones: Parece razonable incorporar y mejorar las experiencias en línea fomentando el desarrollo de competencias socioemocionales para este contexto. Se examinan las repercusiones para el ámbito educativo.

Abstract Introduction: Socio-emotional competencies in face-to-face communication are an essential tool for personal and social adjustment from an early age. Online communication has become equally important, especially in adolescence. It is known that its characteristics require specific skills, but there is a lack of studies that analyse the relationship that exists between generally acquired socio-emotional skills and those necessary for online communication. Hence, this research is focused on analysing how offline socio-emotional skills predict these online competencies in girls and boys in a differentiated way. Method: This study included 478 Secondary Education students between 10 and 14 years of age. This is a cross-sectional study that used Path Analysis differentiated by sex, with intentional and non-probabilistic sampling. To assess socio-emotional competencies, we used the ESCQ-21 instrument for generally assessed socio-emotional competencies and the e-COM questionnaire to quantify online socio-emotional competencies. Results: The explanatory model indicates that socio-emotional competencies are partially transferred to the online environment in both sexes, especially Perception and comprehension. Furthermore, it is suggested that E-self-control of impulsiveness and E-emotional autonomy require specific development in that environment. Conclusions: It seems reasonable to include online experiences that encourage development in online spaces. The repercussions for the educational field are analysed.

Abstract The main coffee disease worldwide is rust by Hemileia vastatrix, which causes losses of up to 40% in production. Biological control with antagonistic fungi represents a management option compatible with the environment. Trichoderma and Lecanicillium strains native to the coffee producing areas of Nayarit were isolated and evaluated under field conditions. In general, Lecanicillium strains showed better performance than Trichoderma strains, with the exception of strain 39, in protecting coffee leaves against Hemileia vastatrix rust, with 80 % effectiveness and reductions in the damage scale at 1 and 2. Therefore, these strains represent a good option for the biological control of Hemileia vastatrix in the study area. Besides, if combat measures are not applied, the infestation percentages reach 85 % and the damage levels are 3 and 4, causing severe coffee cultivation production losses.

Resumen La principal enfermedad del café a nivel mundial es la roya causada por Hemileia vastatrix, que ocasiona pérdidas hasta del 40 % en la producción. El control biológico con hongos antagonistas representa una opción de manejo compatible con el ambiente. Se aislaron y evaluaron en condiciones de campo cepas de Trichoderma y Lecanicillium nativas de la zona cafetalera de Nayarit. En general las cepas de Lecanicillium presentaron mejor desempeño que las de Trichoderma con excepción de la cepa 39 en la protección de las hojas de café contra la roya con efectividades del 80 % y reducciones en la escala de daño a 1 y 2. Por lo anterior, estas cepas representan una buena opción en el control biológico de Hemileia vastatrix, en el área de estudio. Por otra parte, si no se aplican medidas de combate los porcentajes de infestación llegan al 85 % y los niveles de daño son del 3 y 4, provocando graves pérdidas en la producción del cultivo del café.

RESUMO O potássio é um nutriente com potencial para aumentar a tolerância das plantas ao estresse salino. O objetivo deste estudo foi avaliar a produção e a qualidade da couve submetida ao estresse salino e às concentrações de potássio em ambiente protegido. O delineamento experimental adotado foi em parcela subdivididas, sendo as parcelas compostas por cinco soluções nutritivas (tratamento controle, S1 - solução nutritiva padrão usando água de baixa salinidade, 0,5 dS m-1 (500 mg L-1 de KNO3), e quatro soluções nutritivas preparadas em águas salobras (3,5 dS m-1) contendo quatro concentrações de KNO3 (S2 - 500 mg L-1, S3 - 625 mg L-1, S4 - 750 mg L-1, S5 - 1.000 mg L-1). As subparcelas foram representadas por duas cultivares de couve folha (Manteiga e Manteiga da Geórgia). Foram avaliadas as variáveis de produção de folhas e qualidades físicas e químicas pós-colheita. A solução nutritiva salinizada com adição de NaCl (S2) reduziu a produção, mas não reduziu a qualidade pós-colheita da couve folha. A adição extra de KNO3 em 25% (S3) foi eficiente para reduzir o efeito deletério do estresse salino sobre as variáveis comprimento de folha, área foliar, produção e número de maços. A cv. Manteiga da Geórgia se mostrou mais tolerante ao estresse salino que a cv. Manteiga. protegido subdivididas tratamento controle S salinidade 05 0 5 0, m1 m 1 m- (50 L1 L L- KNO3, KNO , KNO3) 3,5 35 3 (3, S2 (S 50 L1, 1, 62 75 1000 000 1.00 L1. . L-1) Geórgia. Geórgia) póscolheita. póscolheita pós colheita. colheita 25 (S3 foliar maços cv (5 3, (3 6 7 100 00 1.0 2 ( 10 1.

ABSTRACT Potassium is a nutrient with the potential to increase plant tolerance to salt stress. The aim of this study was to evaluate the production and quality of kale subjected to salt stress and potassium concentrations in a protected environment. The experimental design was in split plots, with plots composed of five nutrient solutions (control treatment, S1 - standard nutrient solution prepared in low-salinity water, 0.5 dS m-1 (500 mg L-1 of KNO3), and four nutrient solutions prepared in brackish waters (3.5 dS m-1) containing four concentrations of KNO3 (S2 - 500 mg L-1, S3 - 625 mg L-1, S4 - 750 mg L-1, S5 - 1,000 mg L-1), and subplots represented by two leaf kale cultivars (Manteiga and Manteiga da Geórgia). Leaf production variables and post-harvest physical and chemical qualities were evaluated. The saline nutrient solution with the addition of NaCl (S2) reduced production but did not reduce the post-harvest quality of kale. The extra addition of KNO3 at 25% (S3) was efficient in reducing the deleterious effects of salt stress on the variables leaf length, leaf area, leaf production, and number of bunches. The cv. Manteiga da Geórgia was more tolerant to salt stress than the cv. Manteiga. environment control treatment S lowsalinity low salinity water 05 0 5 0. m1 m 1 m- (50 L1 L L- KNO3, KNO , KNO3) 3.5 35 3 (3. S2 (S 50 L1, 1, 62 75 1000 000 1,00 L-1) Geórgia. . Geórgia) postharvest post harvest evaluated 25 (S3 length area bunches cv (5 3. (3 6 7 100 00 1,0 2 ( 10

ABSTRACT Agriculture requires new alternatives to control pests and diseases; biological controls can be a sustainable alternative for the continued success of this sector. This study was thus carried out to identify yeasts and lactic acid bacteria that have the antagonistic capacity to control three phytopathogens as Botrytis cinerea, Lasiodiplodia theobromae and Alternaria brassicae that have caused significant economic losses in agriculture. Thirteen strains of yeast and seven strains of lactic acid bacteria (LAB) were used to measure the percentage of growth inhibition, production of volatile organic compounds, production of biofilms, and the production of enzymes through a completely randomized design. The yeasts Hanseniaspora opuntiae and Saccharomyces cerevisiae and the LAB Lactococcus lactis and L. brevis stood out for their antagonistic capacity to inhibit the growth of phytopathogens by 66%, 58%, 65% and 39%, respectively. Pest and disease control highly depend on chemical phytosanitary inputs with negative economic, environmental, and social effects. This study demonstrated that the yeast H. opuntiae and the LAB L. brevis have potential as biological controls and has been observed to inhibit growth by more than 39%, providing a sustainable alternative that is less harmful to the environment and human health. To guarantee their effectivity under field conditions, their individual application, consortium application, and concentration, timing, and proper application method must be considered.

ABSTRACT Maize (Zea mays L.) is highly sensitive to salt stress, which poses a threat to its production, especially with predicted increases in salinity due to climate change. Enhancing salt tolerance in maize is critical to mitigating these adverse effects, sustaining its production, and ensuring global food security. This study aimed to evaluate the effects of exogenously applied lipoic acid or Si, alone or in integration with soil application of vermicompost-tea on physio-biochemical components, antioxidant defense systems, growth, and yield of maize plants exposed to salt stress. The application of vermicompost-tea was carried out as a soil treatment incorporated into irrigation water at a concentration of 200 L ha-1, and administered in three equal doses in irrigation water within the last 10 min of drip irrigation. Silicon was applied at a rate of 6 mM using potassium silicate and lipoic acid was applied at 0.1 mM. Silicon and lipoic acid were applied three times at 25, 40, and 55 d after planting. The results indicated that the integrative application of soil-based vermicompost-tea with exogenously applied Si or lipoic acid significantly enhanced all studied parameters. Specifically, the combination of Si with vermicompost-tea exhibited the highest improvement percentage compared to untreated control in chlorophyll a (60.3%), chlorophyll b (72.7%), carotenoids (69.9%), net photosynthetic rate (111%), transpiration rate (134%), stomatal conductance (129.5%), relative water content (28.4%), membrane stability index (44.3%), N (33.4%), P (71.9%), K (39.7%), Ca (89.9%), K/Na (81.2%), total soluble sugars (63.7%), proline (60.1%), ascorbate (44.4%), glutathione (83.5%), and α-tocopherol (72.1%). These positive effects were reflected in enhancing yield traits under salt-affected soil conditions by enhancing plant height (25.1%), number of grains per row (43.3%), number of rows per ear (26.7%), 1000‐grain weight (25.9%), grain yield (45.4%), and biological yield (42.8%) compared to untreated control.

Research that directs the way stakeholders act and how they collaborate is essential when addressing complex environmental challenges in the field of sustainability science. For example, researchers attempting to manage Prosopis invasions through biological control in South Africa have historically faced challenges from stakeholders. In this study we illustrate the importance of stakeholder engagement and social learning by outlining the collaborative efforts of various stakeholders to promote effective, integrative and sustainable management of Prosopis invasions in the Northern Cape, South Africa. Through a community of practice approach, stakeholders worked together over the past half-decade in an attempt to develop a National Strategy for Prosopis management and improve its control. This strategy aimed not only to emphasise the need for integration of biomass use (aimed at offsetting the costs of mechanical clearing and necessary herbicide use) but also to underscore the significance of biocontrol alongside other management approaches. Stakeholders also identified that adequate farm-scale planning is necessary to provide a sense of purpose and assist in monitoring of progress. We worked alongside land managers and experts to develop such plans. The engagement of local champions played a crucial role in facilitating collaboration and learning among stakeholders, emphasising the significance of inclusive approaches in addressing complex sustainability challenges. In addition, we gained an understanding of how to develop the community of practice to enhance collaboration that ensures the implementation of plans to better manage Prosopis. Our findings underscore the necessity of meaningful stakeholder engagement and collaboration in effective invasive species management. By promoting understanding and involvement of diverse stakeholders, initiatives can have a greater impact in addressing broader sustainability issues. SIGNIFICANCE: Our findings highlight the fundamental role of stakeholder collaboration in addressing environmental challenges (e.g. biological invasions), promoting sustainability and fostering social learning. Collaboration facilitates exchange of knowledge, promotes social learning and allows stakeholders to make informed decisions when addressing sustainability issues. Collaborative approaches promote the effectiveness of a community of practice in managing Prosopis invasions in South Africa. Local champions played a pivotal role in facilitating collaboration, bridging communication gaps and promoting inclusive approaches. Sustained stakeholder engagement, transdisciplinary collaborations, effective biological control and market development for biomass products will be essential to improve the sustainable management Prosopis.

SUMMARY: The rapid advancement of the Internet of Things (IoT) has transformed our lives by connecting objects and creating an intelligent environment that drives automation and massive data collection. IoT has overcome challenges through remote monitoring, robotics, and sensors, with powerful wireless technologies opening up possibilities in various fields, including anatomical techniques. The objective of this study is to demonstrate the feasibility and benefits of integrating IoT technology into the body maceration process by developing a low-cost, accessible, automated maceration control device compatible with theArduino IDE programming environment. Using an open-source NodeMCU system based on the ESP-12E chip, which employs the LUA programming language and is compatible with Arduino IDE, we incorporated a microSD card reader module for data storage, a digital temperature sensor (DS18B20), a clock module (DS3231), a magnetic stirrer, and an aquarium heater controlled by a digital thermostat with relay (W1209). To validate the device, we conducted maceration tests on C57BL mice, comparing two groups: Control (n=5; weight=17g) subjected to maceration in 4 liters of water at 10°C without agitation; Experimental (n=5; weight=17g) exposed to maceration in 4 liters of water at 35°C, with agitation at 1500 r/min and water flow renewal at a rate of 900 ml/h. Specimens were evaluated for their degree of maceration, cleaned, weighed, and photographed. The maceration time for the control and experi- mental groups was 730 hours and 60 hours, respectively. In the experimental group, no foul odors, damage, or bone demineralization were detected. The device cost was 60 USD, and programming through the Arduino IDE environment was straightforward. The focus on hardware contributed to accessibility and time savings. The system operated with a conventional power supply and required a water supply, making it adaptable to various configurations with greater capacity. Under this configuration, we effectively controlled the variables (temperature, agitation, and water flow). The NodeMCU-based bone maceration system improved the performance and adaptability of existing systems and increased accessibility by reducing costs, complexity, and foul odors.

RESUMEN: El rápido avance del Internet de las Cosas (IoT) ha transformado nuestras vidas al conectar objetos y crear un entorno inteligente que impulsa la automatización y la recolección masiva de datos. El IoT ha superado desafíos a través del monitoreo remoto, la robótica y los sensores, con tecnologías inalámbricas potentes que abren posibilidades en diversos campos, incluidas la anatomía y las técnicas anatómicas. El objetivo de este estudio fue demostrar la viabilidad y los beneficios de integrar la tecnología IoT en el proceso de maceración cadavérica mediante el desarrollo de un dispositivo de control de maceración automatizado, accesible y de bajo costo, compatible con el entorno de programación Arduino IDE. Usando un sistema NodeMCU de código abierto basado en el chip ESP-12E, que emplea el lenguaje de programación LUA y es compatible con Arduino IDE, incorporamos un módulo lector de tarjetas microSD para el almacenamiento de datos, un sensor de temperatura digital (DS18B20), un módulo de reloj (DS3231), un agitador magnético y un calentador de acuario controlado por un termostato digital con relé (W1209). Para validar el dispositivo, realizamos pruebas de maceración en ratones C57BL, comparando dos grupos: control (n=5; peso=17g) sometido a maceración en 4 litros de agua a 10°C sin agitación; experimental (n=5; peso=17g) expuesto a maceración en 4 litros de agua a 35°C, con agitación a 1500 r/min y renovación del flujo de agua a una velocidad de 900 ml/h. Los especímenes fueron evaluados según su grado de maceración, limpiados, pesados y fotografiados. El tiempo de maceración para los grupos control y experimental fue de 730 horas y 60 horas, respectivamente. En el grupo experimental no se detectaron malos olores, daños ni desmineralización de los huesos. El costo del dispositivo fue de 60 USD y la programación a través del entorno Arduino IDE fue sencilla. El enfoque en el hardware contribuyó a la accesibilidad y al ahorro de tiempo. El sistema operaba con una fuente de alimentación convencional y requería un suministro de agua, lo que lo hace adaptable a diversas configuraciones con mayor capacidad. Bajo esta configuración, controlamos eficazmente las variables (temperatura, agitación y flujo de agua). El sistema de maceración ósea basado en NodeMCU mejoró el rendimiento y la adaptabilidad de los sistemas existentes, y aumentó la accesibilidad al reducir los costos, la complejidad y los malos olores.

O campo digital pode ser visto de modo polissêmico, com dinâmicas de controle e vigilância, mas também como espaço criativo para emergência de novas sociabilidades. O presente trabalho enfatiza a segunda dimensão, investigando as contribuições das culturas digitais para o fortalecimento do campo social da Literatura de Cordel (LC) em suas articulações em redes, e na difusão e visibilidade dos seus atores sociais. A Literatura de Cordel  uma forma de poesia popular tradicional do Nordeste do Brasil, tem exibida uma nova vida nas mídias sociais. Muitos cordelistas divulgam seus projetos no Instagram, Facebook e YouTube para compartilhar seus legados e alcançar maior número de pessoas possível. Nessas mídias sociais circulam versos tradicionais de cordel, mas também adaptações para o ambiente digital, como vídeos de cordelistas recitando poemas, e até mesmo interações em tempo real com os seguidores. Isso tem contribuído para a preservação da tradição, da memória e identidade da LC e para a sua divulgação. O artigo investe na abordagem qualitativa da etnografia com observação participante para analisar os fluxos de interações de alguns cordelistas nas mídias sociais. Como resultado parcial, observa-se que as mídias sociais oferecem plataformas potentes para a divulgação e promoção de obras de Literatura de Cordel; bem como ambiente para encontros dos poetas e cordelistas em geral.

The digital field can be seen in a polysemic way, with dynamics of control and surveillance, but also as a creative space for emerging new sociabilities. This paper emphasizes the second dimension, investigating the contributions of digital cultures to strengthening the social field of Cordel Literature (LC) in its articulations in networks, and in the dissemination and visibility of its social actors. LC, a traditional form of popular poetry from the northeast of Brazil, has been given a new lease of life on social media. Many poets publicize their projects on Instagram, Facebook, and YouTube to share their legacies and reach as many people as possible. In these social media, traditional verses of Literatura de Cordel circulate, but also adaptations for the digital environment, such as videos of poets reciting poems, and even real-time interactions with followers. This has contributed to preserving the tradition, memory, and identity of the LC and its dissemination. This is a qualitative study that uses ethnography and participant observation to analyze the flow of interactions between some poets on social media. As a partial result, it was observed that social media offer powerful platforms for the dissemination and promotion of works of Cordel Literature, as well as an environment for meetings between poets and cordelistas in general.