ABSTRACT: To improve the nutritional and functional quality of agro-industrial waste, his study established a ternary mixture of flours made from pineapple, acerola, and passion fruit waste using the simplex-centroid design, as well as to evaluate their effects on the physicochemical and sensory characteristics of edible ice cream. Using mixture planning, nine ternary flour components were applied to edible ice cream formulations to evaluate acceptance scores for taste, texture, and overall impression attributes, as well as physicochemical parameters (pH, acidity, soluble solids, and color parameters) and microbiological parameters (thermotolerant coliforms at 45 ºC, Enterobacteriaceae and Salmonella). The simplex-centroid design demonstrated that the cubic model best predicts the results for the analyzed sensory attributes. The response surface graphs indicated the area of greatest acceptance in the formulations with the maximum percentage established for the flour with acerola waste. The highest level of acceptability was given to the ternary mixture of 60%, 30%, and 10% pineapple, passion fruit, and acerola flours, respectively. The results indicated that sorbet enriched with flour composed of the ternary mixture is a product with the potential for exploitation, as well as contributing to the reduction of environmental impacts generated by the large and improper disposal of agro-industrial fruit processing residues in the ecosystem. ABSTRACT agroindustrial agro industrial pineapple simplexcentroid simplex centroid planning taste texture attributes pH, pH (pH acidity solids thermotolerant 4 ºC Salmonella. Salmonella . Salmonella) 60 60% 30 30% 10 respectively exploitation ecosystem 6 3 1

RESUMO: Visando a qualidade nutricional e funcional dos resíduos agroindustriais, o estudo teve como objetivo estabelecer a mistura ternária de farinhas elaboradas com resíduos de abacaxi, acerola e maracujá utilizando o delineamento simplex-centroide, assim como avaliar seus efeitos nas características físico-químicas e sensoriais em gelados comestíveis. Com o planejamento de misturas, nove componentes ternários das farinhas foram aplicados em formulações de gelados comestíveis para avaliação dos escores de aceitação para os atributos sabor, textura e impressão global, bem como os parâmetros físico-químicos (pH, acidez, sólidos solúveis e parâmetros de cor) e microbiológicos (coliformes termotolerantes a 45 ºC, Enterobacteriaceae e Salmonella). O delineamento simplex-centroide demonstrou que o modelo cúbico melhor prediz os resultados para os atributos sensoriais analisados. Os gráficos de superfície de resposta indicaram a área de maior aceitação nas formulações com o máximo percentual estabelecido para a farinha com os resíduos de acerola. O maior índice de aceitabilidade foi atribuído a mistura ternária constituída com 60%, 30% e 10% para as farinhas de abacaxi, maracujá e acerola, respectivamente. Os resultados indicaram que o sorbet enriquecido com a farinha composta pela mistura ternária é um produto com potencial para exploração, além de contribuir para a redução dos impactos ambientais gerados pelo grande e inadequado descarte de resíduos do processamento agroindustrial de frutas no ecossistema. RESUMO agroindustriais abacaxi simplexcentroide, simplexcentroide simplex centroide, centroide físicoquímicas físico químicas misturas sabor global físicoquímicos químicos pH, pH (pH acidez cor coliformes 4 ºC Salmonella. Salmonella . Salmonella) analisados 60 60% 30 10 respectivamente exploração ecossistema 6 3 1

Resumen: La presencia de bacterias coliformes en el agua presenta una amenaza potencial a la salud pública, ya que puede incrementar el riesgo de enfermedades infecciosas en la población más vulnerable. El asunto resulta más complicado cuando se desconoce la calidad fisicoquímica y microbiológica del agua que se consume. El presente estudio tiene la finalidad de evaluar el estado actual de la calidad de agua de consumo en tres Organizaciones Territoriales de Base (OTB’s) del Municipio de Vinto-Cochabamba y la relación de dependencia de los parámetros microbiológicos con las OTB’s. Los análisis de laboratorio de 30 muestras tomadas en los diferentes domicilios (10 muestras por OTB) muestran el cumplimiento de los límites máximos permisibles de los parámetros fisicoquímicos (pH, conductividad, alcalinidad y dureza) según la Norma Boliviana 512 (NB 512). Respecto a los parámetros microbiológicos para coliformes totales, de las 30 muestras analizadas solo 11 cumplieron con el límite máximo permisible, a diferencia del parámetro de coliformes termorresistentes, donde 25 de las 30 muestras cumplieron con el límite máximo permisible. Los parámetros microbiológicos fueron sometidos al análisis de chi-cuadrado, dando evidencia suficiente para afirmar con un 95% de confianza que los resultados de coliformes totales y termorresistentes no depende de la OTB (p=0.36). Al contrastar los resultados de agua de consumo domiciliario con la del agua suministrada, puede especularse que la contaminación de origen microbiológico ocurre ya sea en las tuberías de distribución, tanques domiciliarios y/o las prácticas de uso del agua de cada domicilio

Abstract:The presence of coliform bacteria in water presents a potential threat to public health, as it can increase the risk of infectious diseases in the most vulnerable population. The matter is more complicated when the physicochemical and microbiological quality of the water consumed is unknown. The purpose of this study is to evaluate the current state of the quality of drinking water in three Territorial Base Organizations (OTB’s) of the Municipality of Vinto-Cochabamba and the relationship of dependence of the microbiological parameters with the OTB’s. Laboratory analyzes of 30 samples taken from different homes (10 samples per OTB) show compliance with the maximum permissible limits of physicochemical parameters (pH, conductivity, alkalinity and hardness) according to Bolivian Standard 512 (NB 512). Regarding the microbiological parameters for total coliforms, of the 30 samples analyzed, only 11 met the maximum permissible limit, unlike the total coliform parameter, where 25 of the 30 samples met the maximum permissible limit. The microbiological parameters were subjected to chi-square analysis, providing sufficient evidence to affirm with 95% confidence that the results of total and heat-resistant coliforms do not depend on the OTB (p=0.36). By contrasting the results of household water consumption with that of water supplied, it can be speculated that contamination of microbiological origin occurs either in the distribution pipes, household tanks and/or the water use practices of each household.

Abstract Fermentation in a solid medium is a technique used for the production of chitin, pigments, proteins and lipids. In the present work, changes in the chemical and microbiological composition were evaluated in micro silages of shrimp (Litopeneus vanamei) and grasshopper (Sphenarium purpurascens) exoskeletons, fermented with a commercial inoculum of Lactobacillus plantarum AVG-112. The amount of molasses for fermentation, proximal composition and microbiological count of the silages, were determined. The waste was mixed with sugarcane molasses as a carbon source and inoculated using a commercial strain (Lactobacillus plantarum AVG-112). After 5 days of fermentation, both silages presented acceptable physical and chemical characteristics. Total coliforms, molds and yeasts were not present and were inhibited by the ensiling process. We concluded that fermentation in a solid medium has suitable characteristics for use as a food supplement for aquatic organisms.

Resumen La fermentación en medio sólido es una técnica utilizada para la producción de quitina, pigmentos, proteínas y lípidos. En el presente trabajo se evaluaron los cambios en la composición química y microbiológica en micro ensilados de exoesqueletos de camarón (Litopeneus vanamei) y chapulín (Sphenarium purpurascens) fermentados con un inoculo comercial de Lactobacillus plantarum AVG-112. Se determinó la cantidad de melaza para la fermentación, la composición proximal y la cuenta microbiológica de los ensilados. Los desechos se mezclaron con melaza de caña de azúcar como fuente de carbono y se inoculó usando una cepa comercial (Lactobacillus plantarum AVG-112). A los 5 d de fermentación ambos ensilados presentaron características físicas y químicas aceptables. Los coliformes totales, mohos y levaduras no estuvieron presentes siendo inhibidos por el proceso de ensilaje. Concluimos que la fermentación en medio sólido tiene características adecuadas para su utilización como suplemento en alimentos para organismos acuáticos.

Abstract Surveillance indicators of the quality of water for human consumption in the Amazon were analysed from 2016 to 2020 using 185,528 samples from 11 microregions. Of the samples analysed, 93.20% were from urban areas, 66.65% were from the public water supply system (WSS), 31.02% were from the Collective Alternative Solution-CAS, and 2.33% from the Individual Alternative Solution-IAS. There was an increase in the number of records by the WSS, with a downwards trend and fluctuations in records for the CAS and the IAS. The quality indicators of chemical and physical parameters for urban areas were higher than those for rural areas and traditional communities. Most of the samples presented pH values below the recommended level. In the quantification of microbiological parameters, a higher presence of total coliforms and E. coli was identified in samples from rural areas and in traditional communities. In conclusion, there were inadequacies in the chemical, physical and microbiological parameters as well as problems related to the supply, storage and surveillance of water distributed for human consumption. These findings indicate the need to build an agenda for public management to address water insecurity and its likely effects on food insecurity in the region. 201 202 185528 185 528 185,52 1 microregions 9320 93 20 93.20 6665 66 65 66.65 WSS , (WSS) 3102 31 02 31.02 SolutionCAS, SolutionCAS Solution CAS, Solution-CAS 233 2 33 2.33 SolutionIAS. SolutionIAS IAS Solution-IAS communities level E conclusion region 18552 18 52 185,5 932 9 93.2 666 6 66.6 (WSS 310 3 0 31.0 23 2.3 1855 5 185, 93. 66. 31. 2.

Resumo Analisaram-se indicadores de vigilância da qualidade da água para consumo humano no Amazonas, de 2016 a 2020, utilizando 185.528 amostras provenientes de 11 microrregiões. Das amostras analisadas, 93,20% são da área urbana, 66,65% provinham do sistema público (SAA), 31,02% da Solução Alternativa Coletiva (SAC) e 2,33% da Solução de Alternativa Individual (SAI). Observou-se aumento do número de registros pelo SAA, com tendência de queda e oscilações de registros para a SAC e a SAI. Os indicadores de qualidade dos parâmetros químicos e físicos da área urbana foram superiores aos das áreas rurais e de comunidades tradicionais. A maior parte das amostras apresentou valores de pH abaixo do recomendado. Na quantificação dos parâmetros microbiológicos, identificou-se maior presença de coliformes totais e E.coli na área rural e em comunidades tradicionais. Em conclusão, verificaram-se inadequações nos parâmetros químicos, físicos e microbiológicos, assim como problemas relativos ao abastecimento, armazenamento e à vigilância da água distribuída para consumo humano. Tais achados indicam a necessidade de construir uma agenda, pela gestão pública, para o enfrentamento da insegurança hídrica e seus prováveis efeitos sobre a insegurança alimentar existente na região. Analisaramse Analisaram se Amazonas 201 2020 185528 185 528 185.52 1 microrregiões analisadas 9320 93 20 93,20 6665 66 65 66,65 SAA , (SAA) 3102 31 02 31,02 (SAC 233 2 33 2,33 SAI . (SAI) Observouse Observou tradicionais recomendado microbiológicos identificouse identificou Ecoli E coli conclusão verificaramse verificaram abastecimento agenda pública região 202 18552 18 52 185.5 932 9 93,2 666 6 66,6 (SAA 310 3 0 31,0 23 2,3 (SAI 1855 5 185. 93, 66, 31, 2,

Abstract This research allowed us to understand the physical-chemical and microbiological quality of rainwater collected in the downtown area and Medrano neighborhood of the city of Quibdó - Chocó. This population has a high precipitation rate which ranges from 1500mm/year and 8500mm/year, both currently and in the future, although this situation contrasts with the low drinking water coverage, which is only 28.5%. To carry out this study, 5 sampling points were identified, and 4 samples were collected at each point with a periodicity of 1 weekly sampling for 4 weeks, for a total of 20 representative samples. The selected roof tops coverings were made of zinc, asbestos cement, and mantle, and are located in areas of high vehicular and pedestrian activity. The collected samples were analyzed in an accredited laboratory for this purpose. The parameters evaluated for rainwater quality were: apparent color, real color, conductivity, pH, total alkalinity, nitrites, nitrates, total coliforms, and Escherichia Coli. The result of the microbiological analyzes was POSITIVE in 100% of the cases, while 15% of the physical analyses and 34% of the chemical analyses also yielded negative results. Due to the presence mainly of colony-forming units of Escherichia Coli and total coliforms, it was concluded that rainwater in the downtown area and Medrano neighborhood of the city of Quibdó - Chocó, does 5not meet the standards established in the evaluated parameters and required by the regulations, which means that it is not safe for human consumption due to its level of contamination.

Resumen Esta investigación permitió conocer la calidad físico - química y microbiológica del agua lluvia recolectada en la Zona Centro y barrio Medrano de la ciudad de Quibdó - Chocó. Esta población presenta una alta tasa de precipitación la cual oscila entre 1500mm/año y 8500mm/año, tanto en la actualidad como en el futuro, aunque esta situación contraste con la baja cobertura de agua potable que tan solo es del 28,5%. Para realizar dicho estudio se identificaron 5 puntos de muestreo, en cada punto se recolectaron 4 muestras con una periodicidad de 1 muestreo semanal durante 4 semanas, para un total de 20 muestras representativas; las cubiertas seleccionadas fueron de zinc, asbesto cemento y manto; además, están ubicadas en zonas de alta actividad vehicular y peatonal. Las muestras recolectadas fueron analizadas en un laboratorio acreditado para tal fin. Los parámetros evaluados de la calidad del agua lluvia fueron; color aparente, color real, conductividad, pH, alcalinidad total, nitritos, nitratos, Coliformes totales y Escherichia Coli. El resultado de los análisis microbiológicos fue negativo en el 100% de los casos, mientras que el 15% de los análisis físicos y el 34% de los análisis químicos también arrojaron resultados negativos. Debido a la presencia principalmente de unidades formadoras de colonia de Escherichia Coli y Coliformes totales, se concluyó que el agua lluvia en la zona centro y el barrio Medrano de la ciudad de Quibdó - Chocó, no cumple con los estándares establecidos en los parámetros evaluados y requeridos por la norma, lo que significa que no es segura para el consumo humano debido al nivel de contaminación que presenta.

ABSTRACT Introduction: Water supply for industrial use and social consumption decreases due to droughts, overexploitation of groundwater and pollution from agricultural and industrial chemicals. Objective: To evaluate the quality of water for industrial use and social consumption from the supply source belonging to the Central Refinería Ecuador (Sugar Refinery Ecuador). Materials and Methods: The quality of water for industrial use and social consumption was evaluated through the application of testing techniques and methods, in the determination of physical, chemical and microbiological parameters. Results and Discussion: The water quality parameters for industrial use and social consumption evaluated were odor, taste and color of odorless, tasteless and colorless respectively. Total dissolved solids were 553 mg L-1, pH 7,33 pH units, calcium 75 mg L-1, magnesium 45 mg L-1, chloride 45 mg L-1, sulfate of 21 mg L-1, nitrite of 0,03 mg L-1, nitrates of 20,2 mg L-1, total coliforms of 1 222 most probable number per 100 mL and thermotolerant coliforms of 584 most probable number probable per 100 mL. Conclusions: These water quality values are suitable for industrial use, while for social consumption they are not suitable due to microbiological contamination and non-compliance with health protection requirements. Although the physical and chemical values analyzed in water for social consumption are within maximum limits.

RESUMEN Introducción: El suministro de agua para uso industrial y consumo social disminuye debido a las sequías, la sobreexplotación del agua subterránea y la contaminación procedente de productos químicos agrícolas e industriales. Objetivo: Evaluar la calidad del agua para el uso industrial y consumo social de la fuente de abasto perteneciente al Central Refinería Ecuador. Materiales y Métodos: La calidad del agua para uso industrial y consumo social se evaluó mediante la aplicación de técnicas y métodos de ensayos, en la determinación de los parámetros físicos, químicos y microbiológicos. Resultados y Discusión: Los parámetros de calidad del agua para el uso industrial y el consumo social evaluados fueron el olor, el sabor y el color de inodora, insípida e incolora respectivamente. Los sólidos totales disueltos fueron de 553 mg L-1, el pH de 7,33 unidades de pH, el calcio de 75 mg L-1, el magnesio de 45 mg L-1, el cloruro de 45 mg L-1, el sulfato de 21 mg L-1, el nitrito de 0,03 mg L-1, los nitratos de 20,2 mg L-1, los coliformes totales de 1 222 número más probable por 100 mL y los coliformes termotolerantes de 584 número más probable por 100 mL. Conclusiones: Estos valores de calidad del agua para el uso industrial son aptos, mientras que para el consumo social son no aptos por contaminación microbiológica e incumplimiento de los requisitos de protección sanitarios. Aunque los valores físicos y químicos analizados en el agua para consumo social se encuentran dentro de límites máximos.

ABSTRACT Introduction: Nowadays the pollutants produced in water exceed the capabilities of its decontamination mechanisms. It is necessary to evaluate the wastewater belonging to the Central Refinería Ecuador (Sugar Refinery Ecuador). Objective: To evaluate the quality of the wastewater from the Central Refinería Ecuador (Sugar Refinery Ecuador) for its possible use in agricultural irrigation. Materials and Methods: The quality of the wastewater was evaluated through physical, chemical and microbiological tests. Results and Discussion: The main physical, chemical and microbiological results evaluated at two points in time were: temperature 46.6 and 43.6 ºC, electrical conductivity 2 359 and 1 366 µS cm-1, sedimentable solids 8 and 3.50 mL L-1. pH 5.8 and 6.3 pH units, total phosphorus 5 and 3.75 mg L-1, total nitrogen 4.20 and 6.45 mg L-1, chemical oxygen demand 1 832 and 1 742 mg L-1, biochemical oxygen demand 1 671 and 1 451 mg L-1, fats and oils 296.90 and 30.90 mg L-1; cadmium 0.06 and 0.16 mg L-1, total chromium 0.10 and 0.20 mg L-1, copper 1.19 and 1.80 mg L-1, lead 0.43 and 0.55 mg L-1, zinc 1.37 and 2.33 mg L-1, total coliforms 8 800 000 and 160 000 mg L-1 and fecal coliforms 8 800 000 and 1 070 000 mg L-1. Conclusions: The effluent does not comply with the discharge requirements of NC 27, (2012) and is classified of poor quality to be used for sugarcane irrigation according to NC 855, (2011).

RESUMEN Introducción: Hoy en día los contaminantes producidos en el agua superan las capacidades de sus mecanismos de descontaminación. Siendo necesario evaluar las aguas residuales pertenecientes al Central Refinería Ecuador. Objetivo: Evaluar la calidad del agua residual del Central Refinería Ecuador, para su posible aprovechamiento en riego agrícola. Materiales y Métodos: La calidad del agua residual se evaluó mediante ensayos físicos, químicos y microbiológicos. Resultados y Discusión: Los principales resultados físico, químicos y microbiológicos evaluados en dos momentos fueron: temperatura 46,6 y 43,6 ºC, conductividad eléctrica 2 359 y 1 366 µS cm-1, sólidos sedimentables 8 y 3,50 mL L-1. pH 5,80 y 6,30 unidades de pH, fósforo total 5 y 3,75 mg L-1, nitrógeno total 4,20 y 6,45 mg L-1, demanda química de oxígeno 1 832 y 1 742 mg L-1, demanda bioquímica de oxígeno 1 671 y 1 451 mg L-1, grasas y aceites 296,9 y 30,9 mg L-1; cadmio 0,06 y 0,16 mg L-1, cromo total 0,10 y 0,20 mg L-1, cobre 1,19 y 1,80 mg L-1, plomo 0,43 y 0,55 mg L-1, zinc 1,37 y 2,33 mg L-1, coliformes totales 8 800 000 y 1 60 000 mg L-1 y coliformes fecales 8 800 000 y 1 070 000 mg L-1. Conclusiones: El efluente no cumple con los requisitos de vertimiento de la NC 27, (2012)y es clasificada de mala calidad para ser aprovechada en el riego de la caña de azúcar según NC 855, (2011).

ABSTRACT The paper analyzes the quality of the water supplied by the potable water treatment plant Altos of Quintero to the city of Santiago de Cuba through the study of five consumers. The quality of the parameters analyzed was determined for pH, color, turbidity, residual free chlorine and determination of total and fecal coliforms, resulting in La Colonia, La Cervecería Hatuey and the Combined Milk, which residual free chlorine and turbidity are outside the norm in some occasions. The effects of the quality of drinking water coming from the potabilizer were mainly the deterioration of the infrastructure of the supply networks and poor storage conditions of the liquid in the above mentioned institutions. Options are presented to ensure that water quality is maintained in the different consumption centers and a predictable model is proposed that allows the maximum time that guarantees the minimum level of allowable residual chlorine that a consumer source should have.

RESUMEN El trabajo analiza la calidad del agua que suministra la potabilizadora Altos de Quintero a la ciudad de Santiago de Cuba mediante el estudio de cinco consumidores. Al agua de los seis centros analizados se les determinó su calidad por la medición de los parámetros: pH, color, turbidez, cloro libre residual y determinación de coliformes totales y fecales, resultando La Colonia, La Cervecería Hatuey y el Combinado Lácteo las entidades en las cuales el cloro libre residual y la turbidez se encuentran fuera de norma en algunas ocasiones. Las afectaciones de la calidad del agua potable procedente de la potabilizadora se deben fundamentalmente al deterioro de la infraestructura de las redes de abastecimiento y a las malas condiciones de almacenamiento del líquido en las instituciones antes mencionadas. Se presentan opciones para garantizar que se mantenga la calidad del agua en los diferentes centros de consumo y se propone un modelo predictivo que posibilita determinar el tiempo máximo que garantiza el mínimo nivel de cloro residual libre permisible que debe disponer una fuente consumidora.

Stormwater harvesting is a promising solution for global freshwater depletion, particularly in tropical regions with abundant rainfall. However, it is not widely used due to the lack of suitable treatment technologies for domestic applications. Multi-stage filtration (MSF) is an effective integrated treatment technology that provides a cost-effective alternative for stormwater treatment. This study investigated MSF's capacity for treating stormwater at different stages. The MSF designed and built comprised the down-flow roughing filter (DRF) and slow sand filter (SSF). The results achieved by the MSF for the treated effluents were: pH (7.1-8.1), temperature (27.6-29.4°C), electrical conductivity (EC) (100-190 µS/cm) and total dissolved solids (TDS) (70-130 mg/L). Turbidity removal efficiency of the MSF was in the range of 36-99% (5.825-164.05 NTU) and the overall average removal efficiency of the MSF was 74%, 90% and 86% for total coliforms (TC) (360-11 800 CFU/ 100 mL), faecal coliforms (FC) (0-1 300 CFU/100 mL) and Enterococcus spp. (120- 1 400 CFU/100 mL), respectively. The study identified stormwater reuse potentials based on international guidelines and benchmarks. For the treated effluent, pH, temperature, EC and TDS were all within the permissible limits for toilet, laundry, bathing, recreational and agricultural water reuse, while turbidity suited agricultural (non-food crop) and restricted urban reuse. 46% of the effluent was suitable for recreational purposes as this satisfied the 50 NTU standard. 62.5% of the effluent satisfied the FC standard for toilets and urinals and agricultural reuse (non-food crop) purposes, while 87.5 % of the effluent satisfied urban reuse purposes (restricted access). 66.67% of the effluent satisfied the Enterococcus spp. standard for agricultural reuse (non-food crop). All treated effluents satisfied the TC bathing standard. This study shows that after minimal disinfection, stormwater effluents offer potential reuse in household applications, thereby reducing potable water demand.

Resumo O presente estudo envolve a análise química e bacteriológica de água de diferentes fontes, ou seja, furo, poços, garrafa e torneira, dos distritos de Peshawar, Mardan, Swat e Kohat da província de Khyber Pakhtunkhwa (KP), Paquistão. De cada distrito, 50 amostras de água (10 amostras de cada fonte), independentemente do status urbano e rural, foram coletadas dessas fontes e analisadas para sulfatos, nitratos, nitritos, cloretos, sólidos solúveis totais e coliformes (E. coli). Os resultados indicaram que a maioria das fontes de água tinha uma contagem inaceitável de E. coli, ou seja, > 34 UFC / 100 mL. As amostras positivas para E. coli foram elevadas no distrito de Mardan, seguido por Kohat, Swat e distrito de Peshawar. Além disso, algumas fontes de água também foram contaminadas quimicamente por diferentes fertilizantes inorgânicos (nitratos/nitritos de sódio, potássio), mas em níveis seguros, enquanto os resíduos agrícolas e industriais (compostos de cloreto e sulfato) estavam em níveis inseguros. Entre todos os distritos, a qualidade da água foi considerada comparativamente mais deteriorada nos distritos de Kohat e Mardan do que nos distritos de Peshawar e Swat. Essas fontes de água química e bacteriologicamente impróprias para beber podem causar problemas à saúde humana.

Abstract The present study involves the chemical and bacteriological analysis of water from different sources i.e., bore, wells, bottle, and tap, from Peshawar, Mardan, Swat and Kohat districts of Khyber Pakhtunkhwa (KP) province, Pakistan. From each district, 50 water samples (10 samples from each source), regardless of urban and rural status, were collected from these sources and analysed for sulphates, nitrates, nitrites, chlorides, total soluble solids and coliforms (E. coli). Results indicated that majority of the water sources had unacceptable E. coli count i.e.> 34 CFU/100mL. E. coli positive samples were high in Mardan District, followed by Kohat, Swat and Peshawar district. Besides this, the some water sources were also chemically contaminated by different inorganic fertilizers (nitrates/nitrites of sodium, potassium) but under safe levels whereas agricultural and industrial wastes (chloride and sulphate compounds) were in unsafe range. Among all districts, the water quality was found comparatively more deteriorated in Kohat and Mardan districts than Peshawar and Swat districts. Such chemically and bacteriologically unfit water sources for drinking and can cause human health problems.

Leachate from a composting plant was characterized and applied as a biofertilizer in lettuce crops. Characteristics of untreated (UE) and anaerobically treated (TE) leachate samples were compared. The pH of TE (8.2) increased in relation to the UE (5.2) due to an increase in ammonia nitrogen in TE (1197 mg L-1) compared to UE (859 mg L-1). Anaerobic treatment was efficient in the removal of organic matter from the leachate, evaluated by the decrease of dissolved organic carbon, biochemical oxygen demand, total organic carbon and total solids. K presented the highest concentration (1743 mg L-1) in TE, followed by Mg (135 mg L-1). Cd, Pb and Cr were present at low concentrations in the samples, 0.047, 0.206 and 0.081 mg L-1, respectively. Salmonella, thermotolerant coliforms and viable helminth eggs were not present in TE, which was applied as a biofertilizer in a lettuce crop and compared to mineral fertilization based on fresh matter and dry matter production. Lettuce production using TE was statistically equivalent to mineral fertilization. Toxic metals were not detected in the lettuce shoots. It was concluded that the anaerobically treated leachate from the composting company has the potential to be used as a biofertilizer. crops (UE (TE 8.2 82 8 2 (8.2 5.2 52 5 (5.2 1197 (119 L1 L 1 L-1 859 (85 L1. . demand solids 1743 (174 135 (13 Cd 0047 0 047 0.047 0206 206 0.20 0081 081 0.08 L1, 1, respectively Salmonella shoots 8. (8. 5. (5. 119 (11 L- 85 (8 174 (17 13 (1 004 04 0.04 020 20 0.2 008 08 0.0 (5 11 ( 17 00 02 0.

Abstract The implementation of effective cleaning and disinfection procedures is crucial to ensure quality control and food safety in food services. This study aimed to evaluate the hygienic conditions in the gastronomy laboratories of a university in Colombia. The study conducted ATP bioluminescence detection and microbiological analysis on various surfaces and food handler's hands. The results indicated the presence of aerobic mesophilic bacteria and total coliforms on the surfaces, but no Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, or Salmonella spp. The cutting-board had the highest microbial counts, while the countertop and serving dish had the lowest. Total coliform counts exceeded the acceptable limits on most surfaces. Correlations between ATP levels and microbial counts were not significant. ATP measurements indicated organic contamination, but not necessarily high microbial loads. The findings emphasize the importance of proper cleaning, disinfection protocols, and personal hygiene practices to prevent cross-contamination and ensure food safety. services Colombia handlers handler s hands coli aureus monocytogenes spp cuttingboard cutting board lowest significant contamination loads protocols crosscontamination cross

Abstract Good manufacturing practices in production facilities are necessary to control microbiological growth and prevent foodborne illnesses. Among the various foods that can be contaminated by intensive handling, meat products such as fresh sausages stand out. With this in mind, the aim of this study was to establish a microbiological profile of the fresh sausages produced in the municipality of Alegrete-RS, Brazil, as part of the SIM/SISBI inspection between 2014 and 2016 and to promote and evaluate the producing establishments in terms of compliance with good manufacturing practices. Samples were regularly taken from 10 businesses in the city and tested for thermotolerant coliform bacteria, sulphitereducing Clostridium, coagulase-positive Staphylococcus and Salmonella spp. In addition, the adequacy of good manufacturing practices was assessed and measures in the form of questionnaires, guidelines and training to improve the production chain were implemented in these companies. Over the years, significant improvements in compliance with good manufacturing practices have been observed in the production units. The microbial count of thermotolerant coliforms, sulphite-reducing Clostridium, coagulase-positive Staphylococcus and Salmonella spp. decreased significantly, indicating that the interventions contributed to improving the microbiological profile of the fresh sausage produced in the community. illnesses handling out mind AlegreteRS, AlegreteRS Alegrete RS, RS Alegrete-RS Brazil SIMSISBI SIM SISBI 201 1 bacteria Clostridium coagulasepositive coagulase positive spp addition questionnaires companies years units coliforms sulphite reducing significantly community 20 2

Resumo Boas Práticas de Fabricação nas agroindústrias são necessárias para controlar a multiplicação de microrganismos e prevenir a ocorrência de doenças veiculadas por alimentos. Dentre os diversos alimentos que podem ser contaminados pela manipulação intensiva, destacam-se os produtos cárneos, como a linguiça frescal. Com base no exposto, objetivou-se avaliar o nível de contaminação de linguiças frescais produzidas no município de Alegrete (RS) no âmbito da fiscalização do SIM/SISBI entre 2014 e 2016 e correlacionar ao atendimento das Boas Práticas de Fabricação (questionários, orientações e treinamentos). Amostras regulares foram coletadas em 10 estabelecimentos da cidade e testadas para coliformes termotolerantes, Clostridium sulfito redutor, Staphylococcus coagulase positiva e Salmonella spp. A quantificação microbiana de coliformes termotolerantes, Clostridium sulfito redutor, Staphylococcus coagulase positiva e Salmonella spp. diminuiu significativamente, indicando que as intervenções contribuíram para melhorar o perfil microbiológico da linguiça frescal produzida na cidade. intensiva destacamse destacam se cárneos exposto objetivouse objetivou RS (RS SIMSISBI SIM SISBI 201 questionários, questionários (questionários treinamentos. treinamentos . treinamentos) 1 termotolerantes redutor spp significativamente 20 2

Abstract Solar Water Disinfection, known as SODIS, is a treatment method involving the exposure of water to solar radiation (UVA light and heat) to eliminate pathogenic microorganisms. Organic coagulants extracted from Moringa oleifera (T1) and Salvia hispanica (T2) were used in conjunction with the filtration process as auxiliaries. This process included sand filters with particle sizes ranging from 0.25 mm to 0.850 mm and a non-woven geotextile placed at its base. After undergoing the coagulation/flocculation/sedimentation/filtration process, the samples were exposed to sunlight in a solar concentrator using bottles painted on half of their surface (PR) and unpainted bottles (BR). The analyzed parameters included physicochemical properties such as pH, electrical conductivity, turbidity, and apparent color, as well as microbiological parameters, including E. coli and total coliforms. The results of physicochemical analyses demonstrated the superiority of T1 in terms of turbidity and apparent color. Regarding microbiological analyses, they showed the effective removal of E. coli and total coliforms, with 100% inactivation observed in the samples. It was observed that the applied treatment sequence significantly reduced the microbiological load of the samples; however, it cannot be stated with certainty whether the coagulants exerted any influence on this result. Disinfection SODIS UVA heat microorganisms T (T1 T2 (T2 auxiliaries 025 0 25 0.2 0850 850 0.85 nonwoven non woven base coagulationflocculationsedimentationfiltration coagulation flocculation sedimentation PR (PR BR. BR . (BR) pH conductivity color E coliforms 100 however result (T 02 2 0. 085 85 0.8 (BR 10 08 8 1

Resumo O sistema de desinfecção solar, conhecido como SODIS (Solar Water Disinfection), é um método que envolve a exposição da água à radiação solar (luz UVA e calor) para eliminar microrganismos patogênicos. Coagulantes orgânicos extraídos da Moringa oleifera (T1) e Salvia hispanica (T2) foram usados em conjunto com o processo de filtração como auxiliares. Esse processo incluiu filtros de areia com tamanhos de partícula variando de 0,25 mm a 0,850 mm e uma manta geotêxtil não tecida colocada em sua base. Após passarem pelo processo de coagulação/floculação/sedimentação/filtração, as amostras foram expostas ao sol em um concentrador solar usando garrafas pintadas na metade de sua superfície (PR) e garrafas não pintadas (BR). Os parâmetros analisados incluíram propriedades físico-químicas como pH, condutividade elétrica, turbidez e cor aparente, bem como parâmetros microbiológicos, incluindo E. coli e coliformes totais. Os resultados das análises físico-químicas demonstraram a superioridade de T1 em termos de turbidez e cor aparente. Em relação às análises microbiológicas, as análises demostraram a remoção eficaz de E. coli e coliformes totais, com 100% de inativação observada nas amostras. Portanto, foi observado que a sequência de tratamento aplicada reduziu significativamente a carga microbiológica das amostras; contudo, não se pode afirmar com certeza se os coagulantes exerceram alguma influência nesse resultado. Solar Disinfection, Disinfection , Disinfection) luz calor patogênicos T (T1 T2 (T2 auxiliares 025 0 25 0,2 0850 850 0,85 base coagulaçãofloculaçãosedimentaçãofiltração coagulação floculação sedimentação coagulação/floculação/sedimentação/filtração PR (PR BR. BR . (BR) físicoquímicas físico químicas pH elétrica aparente microbiológicos E totais microbiológicas 100 Portanto contudo resultado (T 02 2 0, 085 85 0,8 (BR 10 08 8 1

PEO/TiP fibers were obtained using the Solution Blow Spinning (SBS) apparatus and heat treated to produce TiO2 fibers. The morphological and structural characteristics were assessed using SEM and X-ray diffraction. The fibers, with a thickness of 12 μm, showed a change in crystalline structure with heat treatment. At temperatures as low as 800 °C, only the anatase phase was identified, while at 900 °C, both anatase and rutile phases coexisted. The addition of TiP to the polymer matrix reduced the initial breakdown temperature, and the DSC curves showed exothermic peaks due to the amorphous phase transition to TiO2/anatase. The fibers' photocatalytic capacity was tested, revealing that TiO2-fibers in the anatase phase achieved 97% degradation of Rhodamine-B dye in 40 minutes. The study found that the biocide efficacy of TiO2-fibers depends on their heat treatment. Fibers with anatase/rutile or pure rutile phases did not show significant efficiency. However, fibers treated at 600°C with pure anatase phase were more effective in eliminating E. coli and total coliforms. Finally, we can state that the TiO2 fibers obtained in this work using the SBS technique can be used to produce filters to purify water contaminated by pathogens dangerous to human health or even to purify the air. PEOTiP PEO (SBS TiO Xray X ray diffraction 1 μm treatment 80 C °C identified 90 coexisted temperature TiO2anatase TiOanatase TiO2/anatase tested TiO2fibers TiOfibers 97 RhodamineB Rhodamine B 4 minutes anataserutile efficiency However 600C 600 E coliforms Finally air 8 9 60 6