Abstract Sleep disorders are common in children and affect neurological development with important cognitive, emotional and behavioral repercussions. There is a high prevalence of sleep disorders (SD) in neurodevelop mental disorders (NDD) such as autism spectrum dis order (ASD) and attention-deficit hyperactivity disorder (ADHD). Sleep disorders in pediatric population have a prevalence of 6-25%, while in children with NDD this number rises to 50-80%. In NDDs, higher rates of dif ficulties in falling asleep, nocturnal awakenings and daytime sleepiness are observed. Disturbances in the circadian rhythm as well as respiratory sleep disorders are also observed. Consequently, there is a decrease in alertness for daytime activities with increased be havioral disorders, emotional problems and academic difficulties associated with executive and memory dys functions. Sleep assessment has to be a systemic part in the clinical evaluation of children with NDDs, so as to give a convenient diagnosis and treatment in each case, allowing to improve the quality of life of children and their families.

Resumen Los trastornos del sueño son frecuentes en niños y afectan al desarrollo neurológico, con importante repercusión cognitiva, emocional y conductual. Existe una alta prevalencia de trastornos del sueño (TS) en los trastornos del neurodesarrollo (TND), como trastorno del espectro autista (TEA) y trastorno por déficit de atención con hiperactividad (TDAH). Los TS en población pediá trica tienen una prevalencia del 6-25%, mientras que en los niños con TND esta cifra asciende al 50-80%. En los TND se observa un incremento de las dificultades para conciliar el sueño, de los despertares nocturnos y de la somnolencia diurna. Así mismo, presentan alteracio nes del ritmo circadiano y trastornos respiratorios del sueño. Como consecuencia se produce una reducción de la alerta para las actividades diarias con incremento de trastornos conductuales, problemas emocionales y dificultades académicas asociadas a disfunciones eje cutivas y de memoria. La evaluación del sueño debe formar parte sistemática en la valoración clínica de los niños con TND, con el fin de realizar un diagnóstico y un tratamiento adecuados a cada caso, permitiendo mejorar la calidad de vida del niño y de su familia.

Abstract Daily rhythms of behavior and their synchronization in relevant social contexts are fundamental for the survival and reproductive success of all animal species. South American annual fish are adapted to extreme environmental conditions, where the ponds they inhabit dry out as the year progresses, while engaging in reproductive behavior from sexual maturity to death. The ever-changing environmental cycles these species are subjected to makes them an excellent model for studying the expression of biological rhythms in nature. In this work we show for the first time that Garcialebias reicherti, an annual fish native to Uruguay shows daily rhythms in both their locomotor and reproductive behavior. This species shows diurnal behavioral patterns, with neither sex nor reproductive context affecting the phase relationship between the light/dark cycle and activity. However, reproductive context modulates the amount of locomotor activity and leads to synchronization between members of the dyads, while introducing a second behavioral rhythm for reproductive events. Reproductive context emerges as a significant modulator of rhythmic behavior, driving circadian rhythms synchronization alongside environmental zeitgebers, while illuminating the complexity of physiological and behavioral coordination. conditions progresses death everchanging ever changing nature reicherti patterns lightdark light dark However dyads events zeitgebers coordination

Resumen Los ritmos diarios de la conducta y su sincronización en contextos sociales relevantes son fundamentales para la supervivencia y el éxito reproductivo. Los peces anuales sudamericanos están adaptados a condiciones ambientales extremas, donde los charcos que habitan se secan a medida que avanza el año, mientras despliegan conducta reproductiva desde la madurez sexual hasta su muerte. Los ciclos ambientales extremos a los que estas especies están expuestas las convierten en un modelo excelente para estudiar la expresión de los ritmos biológicos en la naturaleza. En este trabajo mostramos por primera vez que Garcialebias reicherti, un pez anual nativo de Uruguay, muestra ritmos diarios tanto en su comportamiento locomotor como reproductivo. Esta especie muestra patrones de comportamiento diurnos, sin que el sexo ni el contexto reproductivo afecten el enganche de fase entre el fotoperiodo y la conducta. Sin embargo, el contexto reproductivo modula la cantidad de actividad locomotora y promueve la sincronización entre los integrantes de las díadas, al tiempo que introduce el comportamiento reproductivo como otro ritmo conductual. El contexto social emerge como un modulador significativo de la conducta rítmica, funcionando en conjunto con los zitegebers ambientales, poniendo en evidencia una compleja coordinación fisiológica y conductual. extremas año muerte naturaleza reicherti Uruguay diurnos embargo díadas conductual rítmica

Abstract Introduction Spastic hemiparetic cerebral palsy (CP) is the most prevalent type of CP. Children with spastic hemiparesis experience difficulties when using their affected upper extremities, and one effective treatment is the Constraint-Induced Movement Therapy (CIMT). The study of rest-activity patterns provides information on children’s daily activities with spastic hemiparetic CP during the day and sleep. Objective To investigate the effect of CIMT on the rest-activity patterns in children with spastic hemiparetic CP vs in a healthy group. Methods Nonrandomized controlled trial was conducted at the Neuropediatric Center of the Hospital de Clínicas Complex, in Curitiba, Brazil. Children with spastic hemi-paretic CP between 5 and 16 years old participated in the study group and receive the CIMT. The healthy group was composed of children between 5 and 15 years old. Both groups used accelerometer to record rest-activity patterns, that may be studied through nonparametric variables of accelerometer: M10 (an individual’s most active 10h); L5 (an individual’s least active 5h); and RA (relative amplitude of the circadian rest-activity patterns). Results Forty-five children were recruited, and 38 were included in the analyses (19 allocated to each group). In the study group, there was a significant increase in M10 and L5 (p < 0.001) after CIMT. The values of M10 and L5 were significantly higher (p < 0.001) in the healthy group compared to the study group after CIMT. Conclusion Our results showed that children with spastic hemiparetic CP became more active and participant in their daily life during the day as well as more efficient sleeping. (CP extremities ConstraintInduced Constraint Induced . (CIMT) restactivity rest activity childrens s sleep Complex Curitiba Brazil hemi paretic 1 M M1 an individuals individual 10h h 10h) L 5h 5h) relative patterns. patterns) Fortyfive Forty five recruited 3 19 (1 group) p 0.001 0001 0 001 sleeping (CIMT ( 0.00 000 00 0.0 0.

Resumo Introdução A paralisia cerebral (PC) hemiparética espástica é o tipo de PC mais prevalente. Crianças com hemiparesia es-pástica apresentam dificuldades ao usar as extremidades superiores afetadas e um tratamento eficaz é a Terapia por Contensão Induzida (TCI). O estudo dos padrões de atividade-repouso fornece informações sobre as atividades diárias de crianças com PC hemiparética espástica durante o dia e o sono. Objetivo Investigar o efeito da TCI nos padrões de repouso-atividade em crianças com PC hemiparética espástica versus um grupo saudável. Métodos Realizou-se um ensaio controlado não randomizado no Centro de Neuropediatria do Complexo do Hospital de Clínicas, Curitiba, Brasil. Crianças com PC hemi-parética espástica entre 5 e 16 anos participaram do grupo de estudo e receberam a TCI. O grupo saudável foi composto por crianças entre 5 e 15 anos. Ambos os grupos utilizaram um acelerômetro para registrar padrões de atividade-repouso, os quais podem ser estudados através de variáveis não paramé-tricas do acelerômetro: M10 (10h mais ativas de um indivíduo); L5 (5h menos ativas de um indivíduo); e AR (amplitude rela-tiva dos padrões de atividade-repouso). Resultados Foram recrutadas 45 crianças e 38 foram incluídas nas análises (19 alocadas em cada grupo). No grupo de estudo, houve aumento significativo de M10 e L5 (p < 0,001) após TCI. Os valores de M10 e L5 foram significativamente maiores (p < 0,001) no grupo saudável em comparação ao grupo de estudo após TCI. Conclusão Os resultados do presente estudo mostraram que crianças com PC hemiparética espástica tornaram-se mais ativas e participantes de sua vida diária durante o dia, bem como dormiram mais eficientemente. (PC prevalente es pástica . (TCI) atividaderepouso atividade repouso sono repousoatividade Realizouse Realizou se Clínicas Curitiba Brasil hemi parética 1 atividaderepouso, repouso, paramétricas paramé tricas M M1 10h h indivíduo indivíduo) L 5h amplitude relativa rela tiva atividaderepouso. atividade-repouso) 4 3 19 (1 grupo. grupo) p 0,001 0001 0 001 tornaramse tornaram eficientemente (TCI ( 0,00 000 00 0,0 0,

Abstract Animals adapt to the daily changes in their environmental conditions by means of genetically encoded circadian clocks. These clocks, found throughout the tree of life, regulate diverse biological functions, and allow periodical changes in physiology and behaviour. The molecular underpinnings of these clocks have been extensively studied across taxa, revealing a brain-based system that coordinates rhythmic activities through neuronal networks and signalling pathways. Entrainment, the alignment of internal rhythms with external cues or zeitgebers, is crucial for the adaptive value of these internal clocks. While the solar light-dark cycle is a primary zeitgeber for most animals, other relevant cues such as temperature, meal timing, predators, anxiety, fear, physical activity, and social interactions also play roles in entraining circadian clocks. The search of a detailed description of the circadian clocks is a goal for neurobiology and an area of growing societal interests. Moreover, as disruptions in circadian rhythms are implicated in various diseases, understanding the entrainment pathways contributes to developing interventions for improved wellbeing and health outcomes. This review focuses on socially relevant cues, examining their impact on animal physiology and behaviour, and explores the sensory pathways transmitting information to the central clock. life functions behaviour taxa brainbased brain based Entrainment zeitgebers lightdark light dark animals temperature timing predators anxiety fear activity interests Moreover diseases outcomes clock

Pectis brevipedunculata is native species and widely available in dry and semi-arid ecosystems showing high biotechnological potential. The objective of this study was to evaluate the circadian and seasonal chemical variation of the essential oil (EO) of P. brevipedunculata, as well as its acaricide effect on Rhipicephalus microplus larvae. Aerial parts were collected and submitted to the hydrodistillation process, and the chemical composition was determined by gas chromatography mass spectrometry (GC-MS). For the assays with R. microplus, the larval immersion test was performed. The main constituents were citral (75% of the EO), followed by α-pinene and limonene. In the seasonality analysis, the highest yields were in the months of April (2.08%) and August (2.05%), while in the study of circadian rhythm, the percentage was 2.0% at 6 p.m. in the rainy season, and 1.2%, dry season at 6 p.m. Concerning acaricidal activity (50% lethal concentration (LC50)), the April (1.17 mg mL-1), March (1.28 mg mL-1), June (1.37 mg mL-1), and October (1.27 mg mL-1) oils obtained were the most active and assays performed with circadian rhythm revealed in the rain season (April) at 6 p.m. and dry season (September) at 12 a.m. LC50 values of 1.75 and 1.75 mg mL-1, respectively. Additionally, this EO is selective to non-target organisms, i.e., ladybeetles and lacewing. semiarid semi arid potential (EO P larvae process GCMS. GCMS GC MS . (GC-MS) R 75% 75 (75 EO, , EO) αpinene α pinene limonene analysis 2.08% 208 2 08 (2.08% 2.05%, 205 2.05% 05 (2.05%) 20 0 2.0 pm p m p.m 1 1.2% 50% 50 (50 LC50, LC (LC50)) 1.17 117 17 (1.1 mL1, mL1 mL 1.28 128 28 (1.2 1.37 137 37 (1.3 1.27 127 27 mL-1 (April September (September am a a.m LC5 175 1.7 1, respectively Additionally nontarget non target organisms ie i e i.e. lacewing (GC-MS 7 (7 2.08 (2.08 2.05 (2.05% 2. 1.2 5 (5 (LC50) 1.1 11 (1. 1.3 13 3 mL- 1. i.e ( (2.0 (2.05 (LC50 (1 (2. (LC5 (2 (LC

ABSTRACT The mechanisms of ecological segregation involved in the coexistence between Neotropical felids are the key to support strategies for conservation. Due to their inconspicuous and elusive behavior, camera trapping constitute a strategic, non-invasive method to study these species. The present work aimed to evaluate the daily and seasonal activity patterns of four felid species: Leopardus guttulus (Hensel, 1872), L. pardalis (Linnaeus, 1758), L. wiedii (Schinz, 1821) and Puma concolor (Linnaeus, 1771), in the Papagaios-de-Altitude Private Protected Area, state of Santa Catarina, southern Brazil. Data were collected from January 2018 to December 2019, using 25 sites of camera traps among the study area. We collected 624 independent records from L. guttulus (108), L. pardalis (55), L. wiedii (77) and P. concolor (384) in a sampling effort of 12,266 camera-traps/day. All species analysed showed a non-uniform distribution of daily activity, when considering the two years. We report the peak of seasonal activity for all species between the months of June and September, coinciding with the Araucaria nut harvest in the study area, and with the increase in populations of small rodents. We also report a high overlap between the activity patterns of the four species. The daily and seasonal activity patterns of the species in this study seem to reflect the intrinsic dynamics of the Araucaria Forest, as well as possible adaptations to prey availability. conservation behavior strategic noninvasive non invasive Hensel, Hensel (Hensel 1872, 1872 , 1872) L Linnaeus, Linnaeus (Linnaeus 1758, 1758 1758) Schinz, Schinz (Schinz 1821 1771, 1771 1771) PapagaiosdeAltitude Papagaios de Altitude Area Catarina Brazil 201 2019 2 area 62 108, 108 (108) 55, 55 (55) 77 (77 P 384 (384 12266 12 266 12,26 cameratraps/day. cameratrapsday traps/day. day camera-traps/day nonuniform uniform years September rodents Forest availability 187 175 182 177 20 6 10 (108 5 (55 7 (7 38 (38 1226 1 26 12,2 cameratraps cameratraps/day trapsday traps/day 18 17 (10 (5 ( 3 (3 122 12, (1

RESUMO Os mecanismos de segregação ecológica envolvidos na coexistência entre felídeos neotropicais são a chave para subsidiar estratégias para sua conservação. Por apresentarem comportamento inconspícuo e esquivo, as armadilhas fotográficas constituem um método não invasivo estratégico para estudá-los. O presente trabalho buscou avaliar o padrão de atividade diário e sazonal de quatro espécies de felídeos: Leopardus guttulus (Hensel, 1872), L. pardalis (Linnaeus, 1758), L. wiedii (Schinz, 1821) e Puma concolor (Linnaeus, 1771), na RPPN Papagaios-de-Altitude, no estado de Santa Catarina, sul do Brasil. Coletamos os dados através de 25 sítios de armadilhas fotográficas na área de estudo. Obtivemos 624 registros independentes de L. guttulus (108), L. pardalis (55), L. wiedii (77) e P. concolor (384) em um esforço amostral de 12.266 armadilhas/dia. Todas as espécies analisadas apresentaram distribuição de atividade diária não uniforme, quando considerados os dois anos. Relatamos o pico de atividade sazonal de todas as espécies entre os meses de junho a setembro, coincidindo com a safra de pinhão na região, e com o aumento nas populações de pequenos roedores. Também relatamos uma alta sobreposição entre os padrões de atividade das quatro espécies. A atividade diária e sazonal das espécies neste estudo parece refletir as dinâmicas intrínsecas da Floresta Ombrófila Mista, além de possíveis adaptações à disponibilidade de presas. conservação esquivo estudálos. estudálos estudá los. los estudá-los Hensel, Hensel (Hensel 1872, 1872 , 1872) L Linnaeus, Linnaeus (Linnaeus 1758, 1758 1758) Schinz, Schinz (Schinz 1821 1771, 1771 1771) PapagaiosdeAltitude, PapagaiosdeAltitude Papagaios Altitude, Altitude Papagaios-de-Altitude Catarina Brasil 2 62 108, 108 (108) 55, 55 (55) 77 (77 P 384 (384 12266 12 266 12.26 armadilhasdia dia armadilhas/dia uniforme anos setembro região roedores Mista presas 187 175 182 177 6 10 (108 5 (55 7 (7 38 (38 1226 1 26 12.2 18 17 (10 (5 ( 3 (3 122 12. (1

Abstract Neocaridina davidi (Bouvier, 1904) is a shrimp commonly used in aquaculture throughout the world. It is an exotic species in several places. We investigated the preference of N. davidi for four types of shelter (rock, Vesicularia sp., Egeria sp., and wood) and its nictemeral activity variation. Individuals were divided into three experimental groups: males, non-ovigerous females, and ovigerous females. They were placed in tanks with the four shelter types and observed every 3 hours for 72 hours. Neocaridina davidi presented cryptic behavior with 82.1 ± 8.69% of individuals sheltering per observation. Sheltering was higher during the daytime (88.8 ±5.54%) than at nighttime (75.4 ± 5.53%). Vesicularia sp. was the most used shelter followed by wood. The least used shelter type was a rock. Swimming and walking were more frequently observed at nighttime, especially for ovigerous females. We have demonstrated the importance of providing shelter for the maintenance of N. davidi in tanks as well as its higher activity at nighttime. Bouvier, Bouvier (Bouvier 1904 world places N rock, rock (rock sp wood variation groups males nonovigerous non females 7 821 82 1 82. 869 8 69 8.69 observation 88.8 888 88 (88. ±5.54% 554 5 54 75.4 754 75 4 (75. 5.53%. 553 5.53% . 53 5.53%) 190 86 6 8.6 88. (88 ±5.54 55 75. (75 5.53 19 8. (8 ±5.5 (7 5.5 ( ±5. 5. ±5

Abstract Crayfish show locomotor activity modulated by environmental lighting. Yet, little is known about different light spectra stimulation effects on the locomotor activity of crayfish, and laboratory instruments are generally limited to the study of a single animal. As such, this research describes the development and implementation of the Crayfish-Tracking Motion Vision (CTMV) system, as a tool for experimentation. Based on artificial vision, this system allowed for the observation and recording of locomotor activity in one to four Procambarus clarkii crayfish that were placed in individual compartments. The effect of blue or green monochromatic light stimulation was explored in two groups of dark-adapted crayfish (with a total of 15 adult crayfish). Light-emitting-diodes of blue or green spectra were applied for 5 min while locomotor activity in crayfish was monitored in a large container. Both light pulses caused similar reaction times of locomotor activity (2.9 ± 3.5 s). Meanwhile, the displacement score of “blue light” animals (2.1 ± 0.9 m) was lower than that of “green light” animals (2.8 ± 0.6 m). In contrast, speed (1.1 ± 1 cm/s) and acceleration data (0 ± 0.8 cm/s2) of the two groups were similar. Finally, the locomotor activity decreased significantly when container size was decreased. These results showed that the CTMV system is appropriate for measuring the locomotor activities of multiple crayfish and suggested that both monochromatic lights induced different locomotor behavior in the crayfish. It has been postulated that this light-induced reflex activity, is caused by an interplay of the photoreceptor system output with a circadian system modulating light sensitivity and locomotor behavior in the crayfish. It can be inferred that the CTMV system will benefit subsequent studies focused on light-induced reflex activity in crustaceans.

Objective: To characterize rest-activity rhythm in chronic migraine (CM) and to investigate the relationship between this rhythm and depressive and anxiety symptoms in patients with CM. Methods: This was a study of adults aged 20 to 40 years. The rest-activity rhythm of patients with CM (n=23) and non-headache controls (NH, n=23) was assessed by actigraphy for 15 days, and they completed the following assessments: Visual Analogue Scale for pain intensity; Headache Diary; Headache Impact Test-6; Morningness-Eveningness Questionnaire; Pittsburgh Sleep Quality Index; Epworth Sleepiness Scale; Beck Depression Inventory; and State-Trait Anxiety Inventory. Results: Patients with CM showed less activity over 24 hours and more fragmented sleep. Reduced interdaily stability of the rest-activity rhythm was observed, with less robustness of this rhythm in the CM group. Multiple linear regressions revealed a significant association between the rest-activity rhythm and trait anxiety variables in patients with CM, specifically regarding the relative amplitude of the cycle, activity throughout 24 hours and during sleep, and robustness of the rest-activity rhythm. Conclusions: Our findings provide evidence that the robustness of the rest-activity rhythm, activity throughout 24 hours, and sleep fragmentation are associated with trait anxiety in patients with CM. Clinical trial registration: Brazilian Clinical Trials Registry (registration number: RBR-4M5J4S).

Abstract Introduction People with bipolar disorder are at a higher risk of metabolic morbidity and mortality. Chronotype may play a significant role due to its effect on sleep quality, eating patterns, and physical activity. Objective To compare sleep quality, social jetlag, physical activity, and diet, depending on the chronotype of people with bipolar disorder, and to determine the association between these variables. Method Cross-sectional, comparative, correlational study. The sociodemographic and clinical characteristics of subjects were assessed. Chronotype was determined using the Composite Scale of Morningness while sleep quality was evaluated through the Pittsburg Sleep Quality Index. Social jet lag was calculated through the absolute difference between the midpoint of sleep on weekends and workdays. Physical activity was measured through the Bouchard Activity Record and diet was evaluated through a food frequency questionnaire. Comparative analyses were performed between chronotype categories and the association between variables was measured. Results 116 subjects were included. Subjects with evening chronotype reported poorer sleep quality than those with morning chronotype. Eveningness was associated with lower sleep quality scores and more hours of sleep on workdays, as well as with higher consumption of cold meats, calories, and sodium. Trends indicate greater social jet lag and low physical activity levels among evening chronotypes. Discussion and conclusion The effects of chronotype on study variables require further research to clarify this complex relationship and develop educational strategies to promote sleep hygiene, physical activity, and a healthy diet.

Resumen Introducción Las personas con trastorno bipolar corren mayor riesgo de presentar morbimortalidad metabólica. El cronotipo podría desempeñar un rol importante por su efecto sobre la calidad de sueño, los patrones de alimentación y la actividad física. Objetivo Comparar la calidad de sueño, el jet lag social, la actividad física y la dieta en función del cronotipo de personas con trastorno bipolar, así como determinar la asociación entre estas variables. Método Estudio transversal, comparativo y correlacional. Se evaluaron las características sociodemográficas y los antecedentes clínicos de los participantes. El cronotipo se determinó con la Escala Compuesta de Matutinidad y la calidad de sueño con el Índice de Calidad de Sueño Pittsburg. El jet lag social se calculó como la diferencia absoluta entre el punto medio de sueño en días libres y de trabajo. La actividad física se midió con el registro de Bouchard y la dieta con un cuestionario de frecuencia de consumo de alimentos. Se realizaron análisis comparativos entre categorías de cronotipo y se evaluó la asociación entre variables. Resultados Se incluyeron 116 participantes. Las personas de cronotipo vespertino reportaron menor calidad de sueño en comparación con las del cronotipo matutino. La vespertinidad se asoció a puntuaciones de menor calidad de sueño y más horas para dormir en días de trabajo, así como a mayor consumo de embutidos, calorías y sodio. Las tendencias indican mayor jet lag social y bajo nivel de actividad física entre cronotipos vespertinos. Discusión y conclusión Los efectos del cronotipo sobre las variables de estudio requieren más investigación, que aclare esta compleja relación, para desarrollar estrategias educativas de higiene de sueño, actividad física y dieta saludable.

Resumen (Introducción): Los refugios de vida silvestre son cruciales para mejorar la conectividad en el contexto de corredores biológicos, ya que conservan fragmentos de bosque y consecuentemente poblaciones de mamíferos neotropicales, sin embargo, para poder idear estrategias de conservación y manejo es prioritario realizar estudios de campo que validen estos esfuerzos y midan su efectividad en el tiempo. (Objetivo): Generar información de línea base sobre el estado poblacional de mamíferos terrestres medianos y grandes y sus patrones de actividad, que contribuyan a realizar un adecuado manejo y protección de este grupo en el Refugio de Vida Silvestre Lapa Verde. (Metodología): Se utilizó la metodología de foto-trampeo colocando 13 cámaras-trampa por bloque de muestreo, en dos bloques (1 855 ha) durante enero - febrero 2015 y entre diciembre 2015 - enero 2016. (Resultados): El esfuerzo de muestreo fue de 1 584 noches, registrando18 especies con presencia de grandes y medianos depredadores como Panthera onca, Canis latrans, Leopardus pardalis y Leopardus wiedii, así como especies que sirven de presas como Silvilagus sp., Dasypus novemcinctus, Dasyprocta punctata y Cuniculus paca. La especie con mayor IAR fue D. punctata, la riqueza fue similar entre bloques; sin embargo, los patrones de actividad circadiana sí presentaron diferencias entre algunas especies (L. pardalis y D. punctata). (Conclusiones): Especies como jaguares y tapires, que requieren grandes extensiones de tierra para asentarse, utilizan estos bloques de conservación, lo que indica la importancia de la conservación de estos bloques.

Abstract (Introduction): Wildlife refuges are crucial to improve connectivity in the context of biological corridors, as they conserve forest patches and consequently neotropical mammal populations. However, in order to devise conservation and management strategies, it is a priority to carry out field studies that validate these efforts and measure their effectiveness over time. (Objective): To generate baseline information on the population status of medium and large terrestrial mammals and their activity patterns to help the management and protection of the Lapa Verde Wildlife Refuge. (Methods): We analyzed the camera-trapping methodology by placing 13 camera traps per sampling block, divided into two blocks (1 855 ha) during January-February 2015 and December 2015-January 2016. (Results): The sampling effort was 1 584 nights, capturing 18 species with the presence of large and medium predators such as Panthera onca, Canis latrans, Leopardus pardalis, and Leopardus wiedii, as well as species of prey such as Silvilagus sp., Dasypus novemcinctus, Dasyprocta punctata, and Cuniculus paca. The species with the highest RAI was D. punctate. The richness was similar between blocks. However, the patterns of circadian activity differed between some species (L. pardalis and D. punctata). (Conclusions): Species such as jaguars and tapirs, which require large areas of land to settle, use these conservation blocks; this indicates the importance of the conservation of these blocks.

Abstract Vertebrates, overall, present a daily activity pattern when managing their needs, such as foraging, resting or searching for sexual partners. Most of the available information regarding the circadian rhythm in birds comes from controlled laboratory conditions, and little is known about these patterns in the wild. In this study we used camera traps to describe the daily activity patterns of three cracid species in the Pantanal of Rio Negro, Brazil. We had a sampling effort of 9,617 camera trap-days along 231 days (5,544 hours) from September 2013 to May 2014. This resulted in 4,833 independent records of cracids from a total of 7,713 individuals. Crax fasciolata was the species with the most records (nrec = 3,792) and individuals (nind = 5,781), followed by Ortalis canicollis (nrec = 934; nind = 1,758) and Aburria grayi (nrec = 107; nind = 174). None of the species was uniformly distributed throughout the day, thus evidencing a periodization of their activities. The mean vectors of the activity patterns of C. fasciolata, O. canicollis and A. grayi were, respectively, mμ = 10:36 ± 04:26 (SD), mμ = 11:42 ± 03:57 and mμ = 11:44 ± 03:47. We observed a temporal overlap between A. grayi and O. canicollis, whereas C. fasciolata significantly differed from them. Because of their large home ranges, cracids are important indicators of environmental quality, and, as frugivores, they play key roles in the ecological dynamics of forests. In this sense, and given that cracids are notably more susceptible to extinction, the knowledge on their circadian activity patterns may be useful when establishing effective management and conservation strategies.

ABSTRACT The purpose of the study was to evaluate the scientific activity about salivary cortisol. A bibliometric method was applied using MEDLINE and LILACS. The study period extended from 1960 to 2019. The data presented in the study were analyzed with the software VOSviewer and Publish or Perish. The search retrieved 6063 documents from MEDLINE and 47 from LILACS. Increase in the number of publications was notable in the period 2010-2019. The United States had the largest scientific production in MEDLINE with 21% of the documents, whereas Brazil was at the top of the list in LILACS with 70.2%. Authors with a productivity index equal to or greater than 1 (prolific producers) represented 0.93% in MEDLINE and 0% in LILACS. Mean number of authors per publication was 5.12 in MEDLINE and 4.70 in LILACS. Six main research topics were found to be related to salivary cortisol: 1) stress 2) hypothalamic-pituitary-adrenal axis; 3) circadian rhythm; 4) emotions, anxiety, depression; 5) sleep-wake; 6) determining factors. Research on salivary cortisol is on the increase, with gradual improvement in multiauthorship as an expression of collaboration between authors. Future research should focus on interindividual differences and intraindividual variability of salivary cortisol concentration.

RESUMEN El objetivo del presente estudio fue evaluar la actividad científica sobre el cortisol salival. Se aplicó un método bibliométrico utilizando MEDLINE y LILACS. El período estudiado fue de 1960 a 2019. Los datos fueron analizados por los programas VOSviewer y Publish or Perish. La búsqueda localizó 6063 documentos en MEDLINE y 47 en LILCAS. El crecimiento de las publicaciones mostró un aumento notorio en el período 2010-2019. En MEDLINE, Estados Unidos lideró la producción científica con el 21 % de los documentos y en LILACS, Brasil, con el 70,2 %. Los autores con índice de productividad igual o mayor que 1 (grandes productores) alcanzaron el 0,93 % en MEDLINE y en LILACS, 0 %. El número medio de autores por publicación fue de 5,12 en MEDLINE y 4,70 en LILACS. Hubo seis temas principales de investigación relacionada con cortisol salival: 1) estrés; 2) eje hipotalámico-pituitario-suprarrenal; 3) ritmo circadiano; 4) emociones, ansiedad, depresión; 5) vigilia-sueño; 6) factores determinantes. El número de estudios sobre cortisol salival va en aumento y la multiautoría, como expresión de la colaboración entre los autores, también. La investigación futura debe centrarse en las diferencias interindividuales y la variabilidad intraindividual de la concentración del cortisol salival.

Abstract: The natural history and ecology of mammals in the High Andes and Andean cloud forests are poorly known. This work aims to analyze the relative abundance and activity patterns of medium and large-sized mammals in the Andean portions of the Greater Madidi-Tambopata landscape using the camera trap methodology. Between 2012 to 2017, twelve sites were sampled covering an altitudinal gradient from 1,057 to 4,902 masl. A total of 311 digital camera trap stations were installed, and we applied a total effort of 5,144 trap nights (TN). We recorded a total of 21,346 photographs and 1,152 independent events from which we identified 28 species of mammals. The species with the highest relative abundance were Lycalopex culpaeus from 1 to 19.89/100TN, Didelphis pernigra from 0.1 to 10.71/100TN, and Eira barbara from 0.2 to 10.48/100TN. An analysis of activity patterns of the species that presented more than 10 independent events was carried out. The species with clearly nocturnal habits were Conepatus chinga, Cuniculus paca, Cuniculus taczanowskii, Didelphis pernigra, Mazama chunyi, Mazama americana, Dasypus novemcinctus, Didelphis marsupialis, Lagidium viscacia, Lycalopex culpaeus and Leopardus tigrinus, while Dasyprocta variegata, Nasua sp., Eira barbara, Hippocamelus antisensis and Tremarctos ornatus were diurnal; Puma concolor and Neogale mustela were cathemeral. The information obtained in our study responds to information gaps of a poorly studied mammal community and highlights the importance of Andean habitats for the conservation and management of montane species.

Resumen: La historia natural y la ecología de los mamíferos en los hábitats andinos y los bosques nublados de los Andes son poco conocidas. Este trabajo tiene como objetivo analizar la abundancia relativa y los patrones de actividad de mamíferos medianos y grandes en áreas andinas del Gran Paisaje Madidi-Tambopata, se utilizó la metodología de registros y monitoreo con cámara trampa. Entre 2012 y 2017, se muestrearon doce sitios que cubrieron un gradiente altitudinal de 1,057 a 4,902 msnm. Se instalaron un total de 311 estaciones de trampas cámara digitales, se obtuvo un esfuerzo total de 5,144 noches trampa (TN). Se registraron un total de 21,346 fotografías y 1,152 eventos independientes, identificando 28 especies de mamíferos. Las especies con mayor abundancia fueron Lycalopex culpaeus de 1 a 19.89/100TN, Didelphis pernigra de 0.1 a 10.71/100TN y Eira barbara de 0.2 a 10.48/100TN. Se realizó un análisis de patrones de actividad de las especies que presentaron más de 10 eventos independientes. Las especies con hábitos claramente nocturnos fueron Conepatus chinga, Cuniculus paca, Cuniculus taczanowskii, Didelphis pernigra, Mazama chunyi, Mazama americana, Dasypus novemcinctus, Didelphis marsupialis, Lagidium viscacia, Lycalopex culpaeus y Leopardus tigrinus, mientras que Dasyprocta variegata, Nasua sp., Eira barbara, Hippocamelus antisensis y Tremarctos ornatus fueron diurnos. Puma concolor y Neogale mustela fueron catamerales. La información obtenida en este estudio responde al vacío de información de una comunidad de mamíferos poco estudiados y destaca la importancia de los hábitats andinos y montanos para la conservación y manejo de especies de montaña.

Abstract Dietary avocado meal (AM) may improve growth and muscle fatty acid composition in pigs due to the expression of genes. Transcriptome analysis of Longissimus dorsi muscle and liver and the effect of 0, 5 and 10 % AM in the feed of Landrace-Yorkshire pigs on growth, carcass, muscle fatty acid composition and blood metabolites were evaluated. Twenty-four castrated males with an initial weight of 55 ± 3 kg were distributed under a completely random design. Transcriptome analysis was carried out with massive sequencing, with EdgeR and DESeq2 methods for differentially gene expression (DGE; P ≤ 0.01, log2 fold change ≤ 1.5). We observed higher DGE in both tissues, with down log2 fold change, by including 5 or 10 % AM in the diet against AM0. Genes associated with lipid metabolism (down ANO3; up GOS2, MYLIP, PLIN4), growth (down FOS, MT1A, MT1D, PHGDH; up ARNTL) circadian clock (down DBP, NOCT, PER1, PER2, PER3, SIK1; up ARNTL), immune system (down CD163, CRP, OTUD1; up C1QTNF7, NFIL3) and antioxidative activity (up HP and NCF4) were observed. The inclusion of AM in the diets increased daily gain, feed intake, slaughtered body weight, carcass weight and ham (P < 0.05). Back fat was greater for AM10 (P < 0.001). AM in the diet decreased intramuscular fat and had less triglycerides in the blood (P < 0.05). AM10 in the diet modified the gene expression and quality of the IMF, had more linoleic acid, total fatty acids Ω6, ∑polyunsaturated fatty acids, PUFA/SFA, and PUFA/MUFA (P < 0.001).