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Resumen Introducción. La identificación y detección de microorganismos a partir de técnicas moleculares se ha convertido en un insumo de gran ayuda para el diagnóstico de enfermedades y de organismos presentes en los cultivos. Organismos patogénicos, no patogénicos, controladores biológicos y microorganismos utilizados como competidores, antagonistas o mutualistas pueden aislarse de cultivos agrícolas, ornamentales y forestales. Objetivo. Identificar a nivel taxonómico, mediante técnicas moleculares, bacterias y levaduras patogénicas y no patogénicas en cultivos agrícolas, ornamentales y forestales de Costa Rica, y conservar el material en un banco de muestras de ADN. Materiales y métodos. Entre los años 2009 y 2018, el Laboratorio de Técnicas Moleculares del Centro de Investigación en Protección de Cultivos de la Universidad de Costa Rica recibió un total de 181 aislamientos de bacterias y levaduras para detección por medio de PCR tiempo final y tiempo real, e identificación mediante de la secuenciación de regiones específicas. Resultados. Del total de muestras, el 94,2 % se analizó por medio de secuenciación y el 5,8 % por PCR. Mediante detección por PCR en el cultivo de arroz, se identificaron bacterias como Burkholderia spp., Acidovorax avenae y Pseudomonas fuscovaginae. Por medio de la secuenciación de la región parcial de 16S, se identificaron 172 muestras de especies de bacterias y cinco muestras de especies de levaduras con la región ITS del ARN ribosomal 18S. Se identificaron microorganismos aislados a partir de dieciocho especies de plantas agrícolas, ornamentales y forestales; entre estos Pseudomonas, Bacillus y Enterobacter, y en el caso de levaduras, Candida, Pichia y Wickerhamomyces. Conclusión. Esta investigación permitió identificar a nivel taxonómico bacterias y levaduras provenientes de cultivos de Costa Rica. Además, se desarrolló un insumo de consulta y la posibilidad de utilizar a futuro los microorganismos conservados dentro de un banco de muestras de ADN.

Abstract Introduction. The identification and detection of microorganisms using molecular techniques has become a very helpful tool for the disease diagnosis and organisms present in crops. Pathogenic, non-pathogenic organisms, biological controllers and other microorganisms used as competitors, antagonists or mutualists can be isolated from agriculture, ornamental and forest crops. Objective. To identify the taxonomic level, by molecular techniques, pathogenic and non-pathogenic bacteria and yeast isolated in agriculture, ornamental and forest crops in Costa Rica, and preserve the material in a DNA bank. Materials and methods. Between 2009 and 2018, the Molecular Techniques Laboratory at the Plant Protection Research Center, Universidad de Costa Rica, received a total of 181 isolates of bacteria and yeast for detection by end-time and real-time PCR and identification through sequencing of specific regions. Results. Of the total samples, 94.2 % were analyzed by sequencing and 5.8 % by PCR. Using PCR, bacteria species were identified in rice, such as Burkholderia spp., Acidovorax avenae and Pseudomonas fuscovaginae. Through sequencing of the partial 16S region, 172 samples of bacterial species were identified, and five samples of yeast species with the ITS region of the 18S ribosomal RNA. Microorganisms isolated from eighteen species of agricultural, ornamental and forest plants were identified. The genera most identified were Pseudomonas, Bacillus and Enterobacter, and in the case of yeast, Candida, Pichia, and Wickerhamomyces. Conclusion. This research allowed the taxonomic identification of bacteria and yeast from crops in Costa Rica. In addition, a consultation input was developed, along with the possibility of future use of the microorganisms that are preserved at the DNA bank.

https://doi.org/10.15517/am.2024.57347

Primary cutaneous melioidosis - A rare case needing intensive care unit admission
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Tripathy, S Pal, S Gutte, S

Southern African Journal of Critical Care (Online)

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Jul 2024, Volumen 40 Nº 2 Paginas 83 - 85

Resumen: En | Texto: En | PDF: En

BACKGROUND. Melioidosis cases are increasing in Southeast Asia, posing a significant challenge owing to the rising number of diabetic and immune-compromised patients. Pneumonia is the most common presentation of melioidosis, while cutaneous melioidosis is rare. OBJECTIVE. We report a case of primary cutaneous melioidosis (PCM) that eventually required intensive care unit (ICU) management. METHOD. We describe the case of a 33-year-old male with uncontrolled diabetes mellitus who initially presented with a skin lesion, which was followed by gradual multiorgan involvement diagnosed as melioidosis based on culture-positive results from blood and synovial fluid, and needed ICU admission. RESULTS. He was successfully treated with intravenous antibiotics and invasive mechanical ventilation after ICU admission. CONCLUSION. Cutaneous melioidosis can lead to ICU admission and, if untreated, has a high fatality rate. Faster diagnostic methods like VITEK 2 Compact and MALDI TOF substantially reduce delays in initiating required treatment.

https://doi.org/10.7196/SAJCC.2024.v40i2.1362

Spondylodiscitis caused by the Burkholderia cepacia complex
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Candido, Rulian Christi Souza Rodrigues

; Borella, Luiz Fernando Monte

; Ramos, Marcelo de Carvalho

; Conterno, Lucieni Oliveira

; Reis, Fabiano

Revista da Sociedade Brasileira de Medicina Tropical

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2024, Volumen 57 elocation e00911-2024

Resumen: En | Texto: En | PDF: En

https://doi.org/10.1590/0037-8682-0001-2024

Evaluation of Different Heating Systems for the Hydrolysis of Residual Frying Oil Catalyzed by Free and Immobilized Lipase
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Correia, Thais Bazilli de Almeida

; Sousa, Grazielle Pereira Reis de

; Reis, Willian de Souza Matias

; Theodoro, Janaina Martins

; Hidalgo, Vitor Bertolassi

; Santos, Jorge Henrique Vieira

; Rós, Patrícia Caroline Molgero Da

; Pereira, Ernandes Benedito

Brazilian Archives of Biology and Technology

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2024, Volumen 67 elocation e24220625

Resumen: En | Texto: En | PDF: En

Abstract Large quantities of residual cooking oil are being increasingly generated in various sectors, as it is often improperly disposed of and causes serious environmental problems. A commonly used alternative is the use of lipases in enzymatic hydrolysis, since it is rather attractive to several sectors, in addition to allowing the use of products generated thereof, such as: free fatty acids, diacylglycerols, monoacylglycerols and free glycerol, moreover, it is capable of operating in mild temperature and pressure conditions. Therefore, the present study aimed to evaluate alternatives for the hydrolysis of residual frying oil by evaluating the efficiency of different types of supports and heating systems using free and immobilized forms of lipase from Burkholderia cepacia (BCL) and Porcine pancreas (PPL) as biocatalyst. Polyhydroxybutyrate (PHB), niobium oxide (Nb2O5) and chitosan (CHIT) were evaluated as support for immobilizing BCL and PPL lipases, and superior results were found regarding the hydrolytic activity of immobilization derivatives using PHB and Nb2O5. Among the heating systems under evaluation, the highest percentage of residual oil hydrolysis was found using the ultrasound system for both free and immobilized lipases, reaching 57.91% hydrolysis for lipases immobilized on Nb2O5 and 61.11% hydrolysis for the derivative immobilized on PHB. The operational stability of both biocatalysts was evaluated using similar half-life time values for both. Thus, it was observed that the ultrasound system was efficient in improving lipase performance in the hydrolysis of free fatty acids, once this unconventional heating system is quite promising for accelerating such enzymatic reactions. sectors problems thereof acids diacylglycerols glycerol moreover conditions Therefore (BCL (PPL biocatalyst PHB, , (PHB) NbO Nb O (Nb2O5 CHIT (CHIT evaluation 5791 57 91 57.91 Nb2O 6111 61 11 61.11 halflife half life Thus reactions (PHB (Nb2O 579 5 9 57.9 611 6 1 61.1 57. 61.

https://doi.org/10.1590/1678-4324-2024220625

Determination of microbial diversity in the ocular conjunctiva of healthy dogs by total DNA sequencing
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Torikachvili, Marcela

; Silva, Mariana Soares da

; Petersen, Michelle Becker

; Mayer, Fabiana Quoos

; Budaszewski, Renata da Fontoura

; Weber, Matheus Nunes

; Pigatto, João Antonio Tadeu

; Canal, Cláudio Wageck

; Siqueira, Franciele Maboni

Ciência Animal Brasileira

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2024, Volumen 25 elocation 77549E

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo A conjuntiva desempenha papel fundamental na saúde e imunidade ocular e atua como uma barreira à entrada de microrganismos. As infecções conjuntivais são comuns em cães e resultam tanto da invasão de microrganismos patogênicos quanto do crescimento descontrolado da microbiota existente. A maior parte dos dados existentes provém de estudos baseados em métodos de cultura tradicionais. Esses relatos apontam para o predomínio de bactérias gram-positivas, principalmente Staphylococcus spp. O presente estudo analisou a comunidade microbiana presente na conjuntiva ocular de uma população heterogênea de cães sem distúrbios oftalmológicos por sequenciamento de DNA. Após exame oftálmico minucioso, foram coletados suabe conjuntivais de ambos os olhos de 30 cães. Após processamento e extração de ácidos nucleicos, o pool de amostras foi submetido ao sequenciamento shotgun de DNA por meio da plataforma Illumina e analisado no servidor Metagenomic Rapid Annotations using Subsystems Technology (MG-RAST). Foi identificada uma predominância do filo Proteobacteria e dos gêneros Ralstonia e Burkholderia juntamente com uma minoria de fungos, enquanto vírus não foram encontrados. O sequenciamento do DNA microbiano trouxe novos dados sobre o assunto, revelando a presença de organismos não cultiváveis até então desconhecidos como parte do microbioma ocular. existente tradicionais grampositivas, grampositivas gram positivas, positivas gram-positivas spp minucioso 3 nucleicos MGRAST. MGRAST MG RAST . (MG-RAST) fungos encontrados assunto (MG-RAST

Abstract The conjunctiva plays an essential role in eye health and immunity and acts as a barrier to the entry of microorganisms. Conjunctival infections are common in dogs and result from both the invasion of pathogenic microorganisms and the uncontrolled growth of the existing microbiota. Most of the existing data come from studies based on traditional culture methods. These reports indicate the predominance of gram-positive bacteria, especially Staphylococcus spp. In the present study, we analyzed the microbiota present on the conjunctival surface from a heterogeneous dog population without ophthalmological disorders using DNA sequencing. After a thorough ophthalmological examination, conjunctival swabs were collected from both eyes of 30 dogs. After processing and nucleic acid extraction, the sample pool was subjected to shotgun DNA sequencing through the Illumina platform and analyzed via the Metagenomic Rapid Annotations using Subsystems Technology (MGRAST) server. A predominance of the phylum Proteobacteria and the genera Ralstonia and Burkholderia were identified along with a minority of fungi, whereas viruses were not found. Microbial DNA sequencing has provided new data on this subject, revealing the presence of noncultivable organisms that were previously unknown as part of the ocular microbiome. methods grampositive gram positive bacteria spp study examination 3 extraction MGRAST (MGRAST server fungi found subject microbiome

https://doi.org/10.1590/1809-6891v25e-77549e

Assessment of Burkholderia glumae control in rice (Oryza sativa) FEDEARROZ 67, using silver nanoparticles (AgNPs) under greenhouse conditions
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MORALES-BECERRA, CARLOS ERNESTO

; ORTIZ-ROJAS, LUZ YINETH

; CHAVES-BEDOYA, GIOVANNI

Revista Colombiana de Ciencias Hortícolas

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Dic 2023, Volumen 17 Nº 3 elocation e16358

Resumen: Es En | Texto: Es En | PDF: Es | PDF: En

RESUMEN El tizón bacteriano de la panícula, causado por Burkholderia glumae, representa una amenaza significativa para la producción global de arroz, poniendo en peligro la seguridad alimentaria futura. La gravedad de esta enfermedad transmitida por semillas ha sido amplificada por las cambiantes condiciones ambientales, y aún no se han encontrado estrategias de control efectivas ni variedades de arroz completamente resistentes. Esta investigación se llevó a cabo con la variedad de arroz FEDEARROZ 67, originaria de Agua Clara, municipio de Cúcuta, y las pruebas se realizaron bajo condiciones controladas en invernadero en la Universidad Francisco de Paula Santander. Nuestro objetivo era evaluar el potencial de las nanopartículas de plata (AgNPs) sintetizadas electroquímicamente para controlar B. glumae. Empleamos un diseño completamente aleatorio con cinco tratamientos: preventivo, curativo, control positivo, control negativo y control absoluto. Las variables analizadas incluyeron el número de hojas cloróticas, la altura de la planta y el peso. Los análisis estadísticos comprendieron análisis de varianza y pruebas de comparación de medias de Tukey utilizando la versión 2018 de XLSTATS. Nuestros hallazgos revelaron que el tratamiento preventivo con AgNPs a una concentración de 5 ppm mostró efectos fitoprotectores significativos contra B. glumae. Las plantas bajo este tratamiento mostraron menos síntomas cloróticos y mayores alturas y pesos en comparación con los controles. Estos hallazgos resaltan el potencial de la nanotecnología como una herramienta innovadora para combatir enfermedades bacterianas en cultivos esenciales como el arroz, contribuyendo así a un futuro sostenible para la agricultura global.

ABSTRACT Bacterial panicle blight, caused by Burkholderia glumae, represents a significant threat to global rice production, jeopardizing future food security. The severity of this seed-borne disease has been amplified by shifting environmental conditions, yet effective control strategies and fully-resistant rice varieties remain elusive. This research was conducted with the FEDEARROZ 67 rice variety sourced from Agua Clara, Cucuta municipality, and tests were undertaken under controlled greenhouse conditions at Universidad Francisco de Paula Santander. Our objective was to assess the potential of electrochemically synthesized silver nanoparticles (AgNPs) for controlling B. glumae. We employed a completely randomized design with five treatments: preventive, curative, positive control, negative control, and absolute control. Variables analyzed included the number of chlorotic leaves, plant height, and weight. Statistical analyses encompassed variance analysis and Tukey's mean comparison tests using XLSTATS version 2018. Our findings revealed that the preventive treatment with AgNPs at a 5 ppm concentration exhibited significant phytoprotective effects against B. glumae. Plants under this treatment showcased fewer chlorotic symptoms and greater heights and weights compared to controls. These findings underline the potential of nanotechnology as a groundbreaking tool for combating bacterial diseases in essential crops such as rice, thereby contributing to a sustainable future for global agriculture.

https://doi.org/10.17584/rcch.2023v17i3.16358

ESTADO ACTUAL DEL USO DE BIOINSUMOS MICROBIANOS EN COLOMBIA
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Rodríguez Aristizabal, Mónica Alejandra

; Lugo Ramírez, María Camila

Chilean journal of agricultural & animal sciences

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Dic 2023, Volumen 39 Nº 3 Paginas 444 - 456

Resumen: En Es | Texto: En Es | PDF: En | PDF: Es

ABSTRACT The use of bioinputs in agricultural practices has increased in recent years given the socio-environmental crisis experienced in developing countries, being an alternative that arises in response to the need to replace agrochemical products by sustainable products, and thus preventing negative impacts on the environment. Colombia, a country with an agricultural tradition, is not far behind. Currently, there are approximately 375 bioinputs registered by the Colombian Agricultural Institute (ICA), of which 174 are formulations based on microorganisms, which are applied in different production systems and exhibit diverse mechanisms of action. This review analyzed the current status of the use of these microbial bioinputs in the country. It was found that biopesticides are the most widely used bioinputs, regardless of the type of bioinput, and that most formulations use bacteria as the main microorganisms, with Azotobacter sp., Bacillus sp., Burkholderia sp., Lactobacillus sp. and Pseudomonas sp. being the most common genera. Furthermore, it was found that these bioinputs have been mainly applied on productive systems such as fruit trees and cereals. This coincides with the national agricultural survey carried out by the National Administrative Department of Statistics (DANE) in 2019, where it was reported that the greatest agricultural production is concentrated precisely in this type of crops. Accordingly, it can be concluded that the use of bioinputs as an alternative to conventional agricultural practices has been increasing in the country, becoming not only a strategy that promotes sustainability, but also an opportunity for biotechnological development.

RESUMEN El uso de bioinsumos en las prácticas agrícolas ha aumentado en los últimos años dada la crisis socioambiental que se vive en los países en vía de desarrollo, siendo una alternativa que surge en respuesta a la necesidad de cambiar los productos agroquímicos por productos sostenibles, y así evitar impactos negativos en el medio ambiente. Colombia, un país con vocación agrícola, no se queda atrás. En la actualidad, existen registrados ante el Instituto Colombiano Agropecuario (ICA) aproximadamente 375 productos de tipo bioinsumos, de los cuales 174 son formulaciones con base en microorganismos, que se aplican en diferentes sistemas productivos y que evidencian mecanismos de acción diversos. Esta revisión analizó el estado actual del uso de estos bioinsumos microbianos en el país. Se encontró que principalmente se usan bioplaguicidas, y que la mayoría de las formulaciones, independientemente del tipo de bioinsumo, usan bacterias como principales microorganismos, siendo los géneros más comunes Azotobacter sp., Bacillus sp., Burkholderia sp., Lactobacillus sp. y Pseudomonas sp. Además, se encontró que el principal uso de estos bioinsumos se ha orientado a los sistemas productivos como frutales y cereales. Esto coincide con la encuesta nacional agropecuaria realizada por el Departamento Administrativo Nacional de Estadísticas (DANE) en 2019, donde se informó que la mayor producción agrícola se concentra justamente en este tipo de cultivos. De acuerdo con esto, se puede concluir que el uso de bioinsumos como alternativa frente a las prácticas agrícolas convencionales ha venido aumentando en el país, convirtiéndose no sólo en una estrategia que promueve la sostenibilidad, sino también en una oportunidad para el desarrollo biotecnológico.

https://doi.org/10.29393/chjaa39-39eamm20039

Phosphorus-solubilizing bacterial endophytes, Burkholderia silvatlantica L-VT09 and N-VT06, in improving characteristics of acid sulfate soil and pineapple yield
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Khuong, Nguyen Quoc Tuong, Ngo Vinh Xuan, Ly Ngoc Thanh Thu, Le Thi My Quang, Le Thanh Phong, Ngo Thanh

Chilean journal of agricultural research

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Dic 2023, Volumen 83 Nº 6 Paginas 666 - 681

Resumen: En | Texto: En | PDF: En

ABSTRACT Acid sulfate soils are limiting the productivity of pineapples (Ananas comosus (L.) Merr. var. comosus) by lacking available P content. This study aims to evaluate the potency of P-solubilizing bacterial endophytes, Burkholderia silvatlantica L-VT09 and N-VT06, on acid sulfate soil properties and features of pineapples grown in that soil. The two-factor experiment was arranged according to a completely randomized block design. One factor was P fertilizer levels: 25% P, 50% P, 75% P, and 100% P; the other was supplementations of P-solubilizing bacterial endophytes: No supplementations, individual supplementation of L-VT09, individual supplementation of N-VT06, and mixed supplementation of both L-VT09 and N-VT06. The result revealed that supplying the mixture of B. silvatlantica L-VT09 and N-VT06 did improve the soil fertility as increasing available P content and bacterial density (12.6 mg P kg-1 and 3.82 most probable number g-1 dry soil weight, respectively), P uptake (19.2 kg ha-1), growth as boosting plant height (8.30 cm), leaves number per plant (6.60 leaves plant-1), D-leaf length and width (2.80 and 0.32 cm, correspondingly), stem size (3.40 cm in length and 0.44 cm in diameter), yield components as enhancing fruit length and fruit diameter (3.50 and 1.46 cm, sequentially), yield (11.5 t ha-1), and pineapple fruit quality as ameliorating sugar content (1.71 °Brix) and water content (166.4 mL), in comparison with the no bacteria supplied case. Supplying the mixture of B. silvatlantica L-VT09 and N-VT06 altered 75% P but still maintained pineapple yield.

Use of multifunctional microorganisms in corn crop
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Cruz, Dennis R. C.

; Silva, Mariana A.

; Nascente, Adriano S.

; de Filippi, Marta C. C.

; Ferreira, Enderson P. de B.

Revista Caatinga

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Jun 2023, Volumen 36 Nº 2 Paginas 349 - 361

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO Na composição do microbioma do solo tem-se inúmeros microrganismos, capazes de promover o crescimento vegetal, mais conhecidos como microrganismos promotores de crescimento de plantas. Objetivamos determinar os efeitos de microrganismos multifuncionais, isoladamente ou em combinação, na produção de biomassa da parte aérea, raiz e total, nas trocas gasosas, no teor de macronutrientes, componentes de produção e produtividade de grãos de plantas de milho. O experimento foi conduzido em casa de vegetação em delineamento inteiramente casualizado, com quatro repetições. Utilizou-se 26 tratamentos que consistiram na microbiolização isolada ou combinada das sementes de milho com as rizobactérias BRM 32109, BRM 32110 e BRM 63573 (Bacillus sp.), BRM 32111 (Burkholderia cepacea), BRM 32112 (Pseudomonas sp.), BRM 32113 (Serratia marcenses) and BRM 32114 (Serratia sp.), Ab-V5 (Azospirillum brasilense) e BRM 63574 (Azospirillum sp.), um isolado de fungo BRM 53736 (Trichoderma koningiopsis) e um tratamento controle (sem a aplicação de microrganismos). Aos sete e 21 dias, foram realizadas mais duas aplicações dos mesmos tratamentos, no solo e nas plantas, respectivamente. Os microrganismos aplicados isoladamente ou em combinação promoveram incrementos significativos de 49% na biomassa das plantas de milho, 30% nas trocas gasosas, 36% no teor de macronutrientes e 33% na produtividade. Os isolados BRM 32114, Ab-V5, BRM 32110 e BRM 32112 e as combinações BRM 32114 + BRM 53736, BRM 63573 + Ab-V5 e 32114 + BRM 32110 promoveram melhores benefícios ao milho, nos permitindo inferir que o uso de microrganismos benéficos afetam significativamente o desenvolvimento das plantas de milho. temse tem se vegetal multifuncionais aérea total gasosas casualizado repetições Utilizouse Utilizou 2 32109 3211 6357 Bacillus sp., sp sp. , sp.) Burkholderia cepacea, cepacea cepacea) Pseudomonas Serratia marcenses AbV5 AbV Ab V5 V Ab-V Azospirillum brasilense 5373 Trichoderma koningiopsis sem microrganismos. . microrganismos) dias respectivamente 49 30 36 33 AbV5, V5, 3210 321 635 537 4 3 32 63 53 6 5

ABSTRACT In the composition of the soil microbiome, there are numerous microorganisms capable of promoting plant growth, better known as plant growth-promoting microorganisms. The study aimed to determine the effects of multifunctional microorganisms, alone or in combination, on shoot, root, and total biomass production, gas exchange, macronutrient content, yield components, and grain yield of corn plants. The experiment was carried out in a greenhouse in a completely randomized design, with four replications. Twenty-six treatments consisted of isolated or combined microbiolization of corn seeds with the rhizobacteria Bacillus sp. (BRM 32109, BRM 32110, and BRM 63573), Burkholderia cepacea (BRM 32111), Pseudomonas sp. (BRM 32112), Serratia marcenses BRM 32113, Serratia sp. (BRM 32114), Azospirillum brasilense (Ab-V5), and Azospirillum sp. (BRM 63574), an isolated of fungus Trichoderma koningiopsis (BRM 53736), and a control treatment (without the application of microorganisms). At seven and 21 days, two more applications of the same treatments were carried out in the soil and the plants, respectively. The microorganisms applied alone or in combination promoted significant increases of 49% in corn plant biomass, 30% in gas exchange, 36% in macronutrient content, and 33% in grain yield. Isolates BRM 32114, Ab-V5, BRM 32110, and BRM 32112 and the combinations BRM 32114 + BRM 53736, BRM 63573 + Ab-V5, and BRM 32114 + BRM 32110 promoted better benefits to corn, allowing us to infer that the use of beneficial microorganisms significantly affects the development of corn plants. microbiome growth growthpromoting shoot root production exchange content components plants design replications Twentysix Twenty six sp 32109 63573, , 63573) 32111, 32111 32111) 32112, 32112) 32113 32114) AbV5, AbV5 AbV Ab V5 V (Ab-V5) 63574, 63574 63574) 53736 53736) without . microorganisms) 2 days respectively 49 30 36 33 V5, Ab-V5 3211 6357 3210 (Ab-V5 5373 4 3 Ab-V 321 635 (Ab-V 537 32 63 53 6 5

https://doi.org/10.1590/1983-21252023v36n212rc

10.

Phonolite associated with organic compound and potassium solubilizing bacteria in tomato cultivation
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Paredes Filho, Mário Viana

; Silva, Adriano Bortolotti da Florentino, Ligiane Aparecida

Revista Ceres

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Ene 2023, Volumen 70 Nº 1 Paginas 133 - 141

Resumen: En | Texto: En | PDF: En

ABSTRACT The use of rock dust in Brazil has been studied and encouraged to reduce the Brazilian dependence on the foreign market for chemical fertilizers and as an alternative to organic agriculture. The objective of this research was to evaluate the effects of using phonolite rock powder and organic compound, associated with inoculation with the potassium solubilizing bacteria (KSB) of the genus Burkholderia on the chemical characteristics of the soil, macro and micronutrients in the aerial part, and tomato productivity. The experiment was installed in pots in a greenhouse, using a randomized block design in a 5 × 2 factorial arrangement, representing five doses of phonolite added in an organic compound, with the presence or absence of inoculation with the strain 100-13. The nutrient contents in the soil and the aerial part and the production of tomato were evaluated. Differences were observed between the doses of the organic compound and the phonolite associated with the inoculation. The treatments containing the highest doses of an organic compound provided better levels of nutrients in the soil and the aerial part, and in the tomato productivity. The inoculation with the bacterial strain improved the release of potassium from the powder of the phonolite rock. agriculture KSB (KSB productivity greenhouse arrangement 10013. 10013 100 13. 13 100-13 evaluated 1001 10 1 100-1 100-

https://doi.org/10.1590/0034-737x202370010015

11.

Identification of management factors associated with glanders’s occurrence in equids in Brazilian Northeast region glanderss glanders s
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Carvalho, Jéssica de Crasto Souza

; Pinheiro Junior, José Wilton

; Falcão, Marcus Vinícius Dias

; Silva, Karla Patrícia Chaves da

; Santana, Vania Lucia de Assis

; Mota, Rinaldo Aparecido

Ciência Rural

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2023, Volumen 53 Nº 10 elocation e20220316

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: O mormo é uma doença infectocontagiosa, zoonótica, de caráter agudo ou crônico, que acomete principalmente os equídeos, causado por Burkholderia (B.) mallei. De janeiro de 2005 até dezembro de 2017, 697 focos da doença foram registrados no Brasil, sendo que a região Nordeste detém 61,4% (428/697) do total de focos. Objetivou-se realizar um estudo epidemiológico da infecção por Burkholderia mallei em propriedades nos estados de Pernambuco, Paraíba, Ceará e Alagoas. Para tal foi aplicado questionário investigativo em seis fazendas onde houve caso de Mormo e 11 fazendas em que não houve relato entre 2007 e 2011 e realizada análise estatística dos fatores de manejo associados à ocorrência do Mormo no Nordeste do Brasil. Observou-se associação significativa para as seguintes variáveis: objetivo da criação (comércio, esporte e trabalho nas fazendas) (P = 0,023); ausência de informação sobre as formas de transmissão da bactéria (P = 0,034); não realização de exames periódicos para o diagnóstico do mormo (P = 0,029); exigência de exame negativo na compra de equinos (P = 0,06); utilização do sistema de criação extensivo (P = 0,027) e deficiência de higienização das baias (P = 001). Os resultados obtidos devem ser utilizados na implementação de medidas de manejo que minimizem o risco de transmissão da bactéria, reduzindo os casos da doença na região e as perdas econômicas com o mormo. RESUMO infectocontagiosa zoonótica crônico equídeos B. B (B. 200 2017 69 Brasil 614 61 4 61,4 428/697 428697 428 (428/697 Objetivouse Objetivou se Pernambuco Paraíba Alagoas 1 201 Observouse Observou variáveis comércio, comércio (comércio P 0,023 0023 0 023 0,023) 0,034 0034 034 0,034) 0,029 0029 029 0,029) 0,06 006 06 0,06) 0,027 0027 027 001. 001 . 001) (B 20 6 61, 428/69 42869 42 (428/69 0,02 002 02 0,03 003 03 0,0 00 2 428/6 4286 (428/6 0, 428/ (428/ (428 (42 (4 (

ABSTRACT: Glanders is a contagious zoonotic infectious disease characterized by an acute or chronic condition that mainly affects equids and is caused by Burkholderia (B.) mallei. From January 2005 to December 2017, 697 farms with positive cases of this disease were registered in Brazil, with the Northeast region accounting for 61.4% (428/697) of the total number of cases. This study conducted an epidemiological study of B. mallei infections that occurred in farms in the states of Pernambuco, Paraíba, Ceará and Alagoas. For this purpose, investigative questionnaires were applied to six farms where there were outbreaks of glanders and 11 farms where there were no reports of glanders between 2017 and 2021, and statistical analysis of the factors associated with the occurrence of glanders in Northeastern Brazil was performed. Factors such as the purpose of rearing (P = 0.023), lack of information on disease transmission modes (P = 0.034), failure to carry out periodic tests for glanders diagnosis (P = 0,029), no request for glanders tests prior to the purchase of new animals periodic tests for glanders diagnosis (P = 0.029), not requiring a negative test for glanders in the acquisition of animals (P = 0.06), use of extensive breeding systems(P = 0.027), and poor stall hygiene were associated with disease occurrence (P = 0.001). Understanding the factors related to the occurrence of glanders in the Northeast Region of Brazil helps to establish management measures that minimize bacteria transmission, thereby reducing the number of cases in the region and economic losses associated with glanders. ABSTRACT B (B. 200 69 614 61 4 61.4 428/697 428697 428 (428/697 Pernambuco Paraíba Alagoas 1 201 2021 performed P 0.023, 0023 0.023 , 0 023 0.023) 0.034, 0034 0.034 034 0.034) 0,029, 0029 0,029 029 0,029) 0.029, 0.029 0.029) 0.06, 006 0.06 06 0.06) systemsP systems 0.027, 0027 0.027 027 0.027) 0.001. 0001 0.001 . 001 0.001) (B 20 6 61. 428/69 42869 42 (428/69 202 002 0.02 02 003 0.03 03 0,02 00 0.0 000 0.00 2 428/6 4286 (428/6 0,0 0. 428/ (428/ 0, (428 (42 (4 (

https://doi.org/10.1590/0103-8478cr20220316

12.

Pathology, microbiology, and molecular evaluation of tissues from equids serologically positive for Burkholderia mallei in Midwestern Brazil Pathology microbiology
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Rocha, Leandro S.

; Oliveira, Aruaque L.F.

; Arruda, Felipe P.

; Pitchenin, Letícia C.

; Dutra, Valéria

; Nakazato, Luciano

; Furlan, Fernando H.

; Colodel, Edson M.

Pesquisa Veterinária Brasileira

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2023, Volumen 43 elocation e07172

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: Mormo é uma enfermidade causada pela bactéria Burkholderia mallei que acomete primariamente cavalos, mulas e burros. A doença pode causar lesões na pele, pulmões e em diversos outros órgãos, entretanto frequentemente manifesta-se como uma enfermidade assintomática. No Brasil são utilizados testes sorológicos de elevada sensibilidade e especificidade para auxiliar na detecção de anticorpos contra B. mallei e contribuir para controle da doença. Porém, devido à obrigatoriedade da eutanásia de animais sororeagentes, os equídeos com sorologia positiva para B. mallei e assintomáticos geraram grandes embates entre criadores, médicos-veterinários e laboratórios de diagnóstico. Este trabalho esclarece as limitações dos testes diagnósticos complementares para detecção de B. mallei e descreve os achados clínicos, morfológicos e de exames laboratoriais em 24 equídeos, procedentes de diferentes municípios do estado de Mato Grosso, Brasil, que reagiram ao teste de fixação de complemento e foram positivos no teste de “western blotting” para mormo. Foram colhidos dados e amostras de tecidos de 24 equídeos para análise histológica, microbiológica e molecular. Em 23 equídeos não existiam sinais clínicos, alterações morfológicas, isolamento microbiológico ou detecção molecular que caracterizassem infecção por B. mallei. Por outro lado, amostras de um cavalo assintomático e sem alterações lesionais apresentaram amplificação de sequência compatível com B. mallei na PCR. Considerando que a infecção por B. mallei é passível da aplicação de medidas de defesa sanitária animal e que por exigência internacional e da legislação nacional, os resultados sorológicos são ferramentas que devem amparar os procedimentos de saneamento para erradicação da bactéria no estado de Mato Grosso, Brasil. RESUMO cavalos burros pele órgãos manifestase manifesta se assintomática B Porém sororeagentes criadores médicosveterinários médicos veterinários diagnóstico clínicos 2 Grosso western blotting mormo histológica morfológicas lado PCR nacional

ABSTRACT: Glanders is a disease caused by the bacterium Burkholderia mallei that primarily affects horses, mules and donkeys. The disease can cause lesions in the skin, lungs and several other organs. However, it often manifests as an asymptomatic disease. In Brazil, serological tests of high sensitivity and specificity are used to assist in the detection of antibodies against B. mallei and to contribute to the control of the disease. However, due to the mandatory euthanasia of seroreactive animals, equids with positive serology for B. mallei and asymptomatic generated great conflicts between breeders, veterinarians and diagnostic laboratories. This study clarifies the limitations of complementary diagnostic tests for detecting B. mallei. It describes the clinical, morphological and laboratory findings in 24 equines from different municipalities in the Mato Grosso State, Brazil, which reacted to the complement fixation test and were positive in the western blotting test for glanders. Data and tissue samples were collected from 24 horses for histological, microbiological and molecular analysis. In 23 horses, no clinical signs, morphological alterations, microbiological isolation, or molecular detection would characterize B. mallei infection. On the other hand, samples from an asymptomatic horse without lesional alterations showed sequence amplification compatible with B. mallei in the PCR. Considering that the infection by B. mallei is subject to the application of animal sanitary defense measures and that, by international requirement and national legislation, the serological results are tools that should support the sanitation procedures for the error of the bacteria in the Mato Grosso State, Brazil. ABSTRACT donkeys skin organs However Brazil B animals breeders laboratories 2 State glanders histological analysis signs isolation hand PCR legislation

https://doi.org/10.1590/1678-5150-pvb-7172

13.

Brazilian guidelines for the pharmacological treatment of the pulmonary symptoms of cystic fibrosis. Official document of the Sociedade Brasileira de Pneumologia e Tisiologia (SBPT, Brazilian Thoracic Association) fibrosis SBPT, SBPT (SBPT Association
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Athanazio, Rodrigo Abensur

; Tanni, Suzana Erico

; Ferreira, Juliana

; Dalcin, Paulo de Tarso Roth

; Fuccio, Marcelo B de

; Esposito, Concetta

; Canan, Mariane Gonçalves Martynychen

; Coelho, Liana Sousa

; Firmida, Mônica de Cássia

; Almeida, Marina Buarque de

; Marostica, Paulo José Cauduro

; Monte, Luciana de Freitas Velloso

; Souza, Edna Lúcia

; Pinto, Leonardo Araujo

; Rached, Samia Zahi

; Oliveira, Verônica Stasiak Bednarczuk de

; Riedi, Carlos Antonio

; Silva Filho, Luiz Vicente Ribeiro Ferreira da

Jornal Brasileiro de Pneumologia

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2023, Volumen 49 Nº 2 elocation e20230040

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO A fibrose cística (FC) é uma doença genética que resulta em disfunção da proteína reguladora de condutância transmembrana da FC (CFTR), que é um canal de cloro e bicarbonato expresso na porção apical de células epiteliais de diversos órgãos. A disfunção dessa proteína resulta em manifestações clínicas diversas, envolvendo primariamente os sistemas respiratório e gastrointestinal com redução da qualidade e expectativa de vida. A FC ainda é uma patologia incurável, porém o horizonte terapêutico e prognóstico é hoje totalmente distinto e muito mais favorável. O objetivo destas diretrizes foi definir recomendações brasileiras baseadas em evidências em relação ao emprego de agentes farmacológicos no tratamento pulmonar da FC. As perguntas PICO (acrônimo baseado em perguntas referentes aos Pacientes de interesse, Intervenção a ser estudada, Comparação da intervenção e Outcome [desfecho] de interesse) abordaram aspectos relativos ao uso de moduladores de CFTR (ivacaftor, lumacaftor + ivacaftor e tezacaftor + ivacaftor), uso de dornase alfa, terapia de erradicação e supressão crônica de Pseudomonas aeruginosa, e erradicação de Staphylococcus aureus resistente a meticilina e do complexo Burkholderia cepacia. Para a formulação das perguntas, um grupo de especialistas brasileiros foi reunido e realizou-se uma revisão sistemática sobre os temas, com meta-análise quando aplicável. Os resultados encontrados foram analisados quanto à força das evidências compiladas, sendo concebidas recomendações seguindo a metodologia GRADE. Os autores acreditam que o presente documento represente um importante avanço a ser incorporado na abordagem de pacientes com FC, objetivando principalmente favorecer seu manejo, podendo se tornar uma ferramenta auxiliar na definição de políticas públicas relacionadas à FC. (FC CFTR, , (CFTR) órgãos diversas vida incurável favorável acrônimo interesse estudada desfecho [desfecho ivacaftor, (ivacaftor ivacaftor) alfa aeruginosa cepacia realizouse realizou temas metaanálise meta análise aplicável compiladas GRADE manejo (CFTR

ABSTRACT Cystic fibrosis (CF) is a genetic disease that results in dysfunction of the CF transmembrane conductance regulator (CFTR) protein, which is a chloride and bicarbonate channel expressed in the apical portion of epithelial cells of various organs. Dysfunction of that protein results in diverse clinical manifestations, primarily involving the respiratory and gastrointestinal systems, impairing quality of life and reducing life expectancy. Although CF is still an incurable pathology, the therapeutic and prognostic perspectives are now totally different and much more favorable. The purpose of these guidelines is to define evidence-based recommendations regarding the use of pharmacological agents in the treatment of the pulmonary symptoms of CF in Brazil. Questions in the Patients of interest, Intervention to be studied, Comparison of interventions, and Outcome of interest (PICO) format were employed to address aspects related to the use of modulators of this protein (ivacaftor, lumacaftor+ivacaftor, and tezacaftor+ivacaftor), use of dornase alfa, eradication therapy and chronic suppression of Pseudomonas aeruginosa, and eradication of methicillin-resistant Staphylococcus aureus and Burkholderia cepacia complex. To formulate the PICO questions, a group of Brazilian specialists was assembled and a systematic review was carried out on the themes, with meta-analysis when applicable. The results obtained were analyzed in terms of the strength of the evidence compiled, the recommendations being devised by employing the GRADE approach. We believe that these guidelines represent a major advance to be incorporated into the approach to patients with CF, mainly aiming to favor the management of the disease, and could become an auxiliary tool in the definition of public policies related to CF. (CF CFTR (CFTR organs manifestations systems expectancy pathology favorable evidencebased based Brazil studied interventions (PICO ivacaftor, ivacaftor (ivacaftor lumacaftorivacaftor lumacaftor lumacaftor+ivacaftor tezacaftor+ivacaftor, tezacaftorivacaftor tezacaftor+ivacaftor , tezacaftor tezacaftor+ivacaftor) alfa aeruginosa methicillinresistant methicillin resistant complex questions themes metaanalysis meta analysis applicable compiled

https://doi.org/10.36416/1806-3756/e20230040

14.

Historical highlights regarding glanders, from 2007 to 2017, in the state of Maranhão, Brazil glanders 200 2017 Maranhão 20 201 2
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Vieira, Ester Clévia dos Santos

; Baquero, Oswaldo Santos

; Mesquita, Eric Takashi Kamakuru de Carvalho

; Bezerra, Nancyleni Pinto Chaves

; Melo, Ferdinan Almeida

; Simas, Ana Karoline Sousa Mendes

; Chaves, Daniel Prazeres

; Coimbra, Viviane Correa Silva

; Ferreira, José Claudio Araújo

; Mota, Rinaldo Aparecido

; Costa, Francisco Borges

Ciência Rural

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2023, Volumen 53 Nº 10 elocation e20210901

Resumen: Pt En | Texto: Pt En | PDF: Pt | PDF: En

RESUMO: O mormo é uma doença infecciosa de equídeos causada por Burkholderia mallei, uma bactéria Gram-negativa intracelular, não móvel e facultativa que pode ser transmitida a outros animais e humanos. O objetivo deste estudo foi descrever os casos de mormo relatados no estado do Maranhão, em uma região de transição Amazônia-Cerrado do Nordeste do Brasil, no período de 2007 a 2017. Um banco de dados em uma planilha eletrônica (Microsoft Excel 2010) foi desenvolvido contendo informações sobre o número de animais positivos de acordo com a idade, sexo, finalidade, ano, mês, municípios e mesorregiões de origem do animal. A análise descritiva foi realizada sobre os dados fornecidos pela Agência Estadual de Defesa Agropecuária do Estado do Maranhão (AGED), e pelo laboratório privado oficial. Como o banco de dados não possui informações sobre animais negativos, possíveis fatores de risco não puderam ser avaliados. Entre o total de 62.555 equídeos foram avaliados com o teste de fixação do complemento (CFT), 59.036 eram cavalos, 2.981 mulas e 538 jumentos. Trinta e cinco (0,06%) amostras reagiram ao CFT. Cinco amostras adicionais foram testadas pela técnica de western blot e três foram positivas. Todos os animais positivos foram cavalos (Equus caballus) da área rural. Os resultados indicam que o mormo pode ser endêmico no estado do Maranhão e uma preocupação de saúde pública. RESUMO mallei Gramnegativa Gram negativa intracelular humanos AmazôniaCerrado Amazônia Cerrado Brasil 200 2017 Microsoft 2010 idade sexo finalidade ano mês animal AGED, AGED , (AGED) oficial negativos 62555 62 555 62.55 CFT, CFT (CFT) 59036 59 036 59.03 2981 2 981 2.98 53 jumentos 0,06% 006 0 06 (0,06% positivas Equus caballus rural pública 20 201 (AGED 6255 6 55 62.5 (CFT 5903 5 03 59.0 298 98 2.9 0,06 00 (0,06 625 62. 590 59. 29 9 2. 0,0 (0,0 0, (0, (0 (

ABSTRACT: Glanders is an infectious disease of equids caused by Burkholderia mallei, a facultative intracellular non-mobile Gram-negative bacterium that can be transmitted to other animals and humans. This study described glanders cases reported in the state of Maranhão, located within an Amazon-Cerrado transition region in northeastern Brazil, from 2007 to 2017. A database in an electronic spreadsheet (Microsoft Excel 2010) was developed containing information on the number of positive animals according to age, sex, purpose, year and month, municipality and mesoregion of origin of the animal. The descriptive analyzis was performed on data provided by the State Agency for Agricultural and Livestock Protection of the state of Maranhão (AGED), and by the official private laboratory. As the database did not have information data about negative animals, possible risk factors could not be evaluated. Among the total of 62,555 equids were evaluated by means of the complement fixation test (CFT), 59,036 were horses, 2,981 mules and 538 donkeys. Thirty-five samples (0.06%) reacted in the CFT. Five additional samples were by the western blot technique and three of them were positive. All the reactive horses (Equuscaballus) were from rural areas. Results presented here indicate that glanders may be endemic in the state of Maranhão and is a public health concern. ABSTRACT mallei nonmobile non mobile Gramnegative Gram humans AmazonCerrado Amazon Cerrado Brazil 200 2017 Microsoft 2010 age sex purpose month animal AGED, AGED , (AGED) laboratory 62555 62 555 62,55 CFT, CFT (CFT) 59036 59 036 59,03 2981 2 981 2,98 53 donkeys Thirtyfive Thirty five 0.06% 006 0 06 (0.06% Equuscaballus (Equuscaballus areas concern 20 201 (AGED 6255 6 55 62,5 (CFT 5903 5 03 59,0 298 98 2,9 0.06 00 (0.06 625 62, 590 59, 29 9 2, 0.0 (0.0 0. (0. (0 (

https://doi.org/10.1590/0103-8478cr20210901

15.

Genome-based taxonomy of Burkholderia sensu lato: Distinguishing closely related species
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Bach, Evelise

; Volpiano, Camila Gazolla Sant’Anna, Fernando Hayashi Passaglia, Luciane Maria Pereira

Genetics and Molecular Biology

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2023, Volumen 46 Nº 3 elocation e20230122

Resumen: En | Texto: En | PDF: En

Abstract The taxonomy of Burkholderia sensu lato (s.l.) has been revisited using genome-based tools, which have helped differentiate closely related species. Many species from this group are indistinguishable through phenotypic traits and 16S rRNA gene sequence analysis. Furthermore, they also exhibit whole-genome Average Nucleotide Identity (ANI) values in the twilight zone for species circumscription (95-96%), which may impair their correct classification. In this work, we provided an updated Burkholderia s.l. taxonomy focusing on closely related species and give other recommendations for those developing genome-based taxonomy studies. We showed that a combination of ANI and digital DNA-DNA hybridization (dDDH) applying the universal cutoff values of 95% and 70%, respectively, successfully discriminates Burkholderia s.l. species. Using genome metrics with this pragmatic criterion, we demonstrated that i) Paraburkholderia insulsa should be considered a later heterotypic synonym of Paraburkholderia fungorum; ii) Paraburkholderia steynii differs from P. terrae by harboring symbiotic genes; iii) some Paraburkholderia are indeed different species based on dDDH values, albeit sharing ANI values close to 95%; iv) some Burkholderia s.l. indeed represent new species from the genomic viewpoint; iv) some genome sequences should be evaluated with care due to quality concerns.

https://doi.org/10.1590/1678-4685-gmb-2023-0122

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