resumo O artigo é um exercício etnográfico da pesca do guaiamum ( Cardisoma ganhumi ) nos manguezais da Baía de Todos os Santos. Tomando como referência os modos de viver e habitar deste crustáceo e dos gaiamunzeiros quilombolas que os capturam como atividade pesqueira, pretendo descrever os efeitos do neoextrativismo nas paisagens pesqueiras da região. A intenção é, por um lado, mostrar práticas, habitabilidades e paisagens associadas à pesca do guaiamum e, por outro, oferecer descrição sobre conflitos e impactos oriundos das infraestruturas agropastoris e petrolíferas nos seus territórios e modos de vida. Objetiva-se, com isso, contribuir para o debate público acerca do marco regulatório da pesca do guaiamum, reforçando a importância da participação dos gaiamunzeiros e pescadores artesanais quilombolas nas tomadas de decisão sobre a preservação da espécie e proteção dos seus ambientes de vida. Santos pesqueira região lado práticas outro vida Objetivase, Objetivase Objetiva se, se Objetiva-se isso

Abstract The article is an ethnographic exercise of fishing for blue land crab ( Cardisoma ganhumi ) in the mangroves of Baía de Todos os Santos. Taking as a reference the ways of living and inhabiting this crustacean and the gaiamunzeiros that capture them as a fishing activity, it aims to describe the effects of neoextractivism in the fishing landscapes of the region. The intention is, on the one hand, to show practices, habitability and landscapes associated with blue land crab fishing and, on the other hand, to offer a description of conflicts and impacts arising from agropastoral and oil infrastructures in their territories and ways of life. Thus, the article also aims to contribute to the public debate on the regulatory framework for blue land crab fisheries, reinforcing the importance of the participation of gaiamunzeiros and quilombola artisanal fishermen in decision-making on the preservation of the species and protection of their living environments. Santos activity region hand practices life Thus fisheries decisionmaking decision making environments

Resumo A quitina e seus produtos derivados têm imenso valor econômico devido ao seu papel vital em várias atividades biológicas, bem como em aplicações biomédicas e industriais. Insetos, microrganismos e crustáceos são o principal suprimento de quitina, mas a casca dos crustáceos como camarão, krill, lagosta e caranguejo são as principais fontes comerciais. O conteúdo de quitina de um indivíduo varia dependendo das estruturas que possuem o polímero e da espécie. Neste estudo, as cascas de caranguejos comestíveis (Callinectes sapidus) foram desmineralizadas e desproteinizadas, resultando em 13,8% (peso seco) de recuperação de quitina a partir de resíduos de quitina. As análises de FTIR e XRD do bruto experimental, bem como das quitinas purificadas, revelaram que ambas eram muito comparáveis aos controles adquiridos comercialmente. O pré-tratamento com ácido cedeu 54 g de quitina coloidal que resultou em 1.080% da quitina bruta. A quitina coloidal foi analisada para isolamento de 85 isolados bacterianos quitinolíticos de diferentes fontes. A zona de eliminação foi exibida pelos 35 isolados (41,17%) que sucederam seu crescimento a pH 7 em meio de ágar de quitina coloidal. A atividade quitinolítica máxima, ou seja, 301,55 U / ml, foi exibida pelo isolado JF70 quando cultivado em quitina extraída contendo carbono e nitrogênio. O estudo mostrou que resíduos de caranguejos azuis podem ser utilizados para extração de quitina e isolamento de bactérias quitinolíticas que podem ser usadas para degradar resíduos de quitina, resolver a poluição ambiental e também para fins industriais.

Abstract Chitin and its derived products have immense economic value due to their vital role in various biological activities as well as biomedical and industrial application. Insects, microorganism and crustaceans are the main supply of chitin but the crustaceans shell like shrimp, krill, lobsters and crabs are the main commercial sources. Chitin content of an individual varies depending on the structures possessing the polymer and the species. In this study edible crabs’ shells (Callinectes sapidus) were demineralized and deproteinized resulting in 13.8% (dry weight) chitin recovery from chitin wastes. FTIR and XRD analyses of the experimental crude as well as purified chitins revealed that both were much comparable to the commercially purchased controls. The acid pretreatment ceded 54g of colloidal chitin that resulted in 1080% of the crude chitin. The colloidal chitin was exploited for isolation of eighty five chitinolytic bacterial isolates from different sources. Zone of clearance was displayed by the thirty five isolates (41.17%) succeeding their growth at pH 7 on colloidal chitin agar medium. Maximum chitinolytic activity i.e. 301.55 U/ml was exhibited by isolate JF70 when cultivated in extracted chitin containing both carbon and nitrogen. The study showed wastes of blue crabs can be utilized for extraction of chitin and isolation of chitinolytic bacteria that can be used to degrade chitin waste, resolve environmental pollution as well as industrial purpose.

ABSTRACT The objective of this work was to evaluate the acute toxicity of the commercial herbicides Karmex® (diuron: DCMU 3-(3,4-dichlorophenyl)-1,1-dimethylurea) and Herbipol® (glyphosate: N-(phosphonomethyl) glycine) on the megalopae of Callinectes sapidus and measure their relative abundance in the association of larval and postlarval species of crustaceans from the estuary of the Jamapa River, Veracruz. Collections were made overnight using white light traps. Likewise, dissolved oxygen, pH, temperature, total dissolved solids, and salinity were measured in situ. It was found that the association of larvae and postlarvae consisted of Macrobrachium acanthurus, M. olfersii, Potimirim mexicana, megalopae of C. sapidus and Armases ricordi, and zoeas of Brachyura. In total, 559 and 1057 C. sapidus megalopae were collected in November and March 2019, respectively. Dissolved oxygen was 6 and 7 mg L−1, pH was 7 and 8, the temperature was 26 and 27°C, total dissolved solids were 700 and 1500 ppm, and salinity was 0.70 and 16 in November and March, respectively. Using Probit analysis, a 96 h LC50 of diuron (Karmex®) was 7.69 ± 1.07 mg L−1 in March and 6.64 ± 0.93 mg L−1 in November. The LC50 for glyphosate (Herbipol®) was 247.83 ± 34.54 mg L−1 in March and 288.18 ± 38.66 3 mg L−1 in November. The blue crab C. sapidus is tolerant to a wide range of physicochemical factors; however, it is sensitive in the postlarval stages, like other species of crustaceans, to the herbicides diuron and glyphosate, for which the megalopae can be used in ecotoxicological studies.

Resumen El cangrejo azul de tierra Cardisoma guanhumi es una especie de interés comercial y está siendo sometida a extracción para el consumo humano. Se distribuye en todo el Caribe desde las Antillas hasta la parte sur en Venezuela y Colombia, con registros, también, en Brasil. Los primeros estudios sobre este cangrejo en Colombia datan de los inicios de la década 1970-1980, los cuales se enfocaban en aspectos ecológicos y biológicos. Los estudios poblacionales en el Caribe colombiano empezaron en el 2000 pero son escasos y algunos no están publicados. Para la isla de San Andrés, el Golfo de Urabá y el departamento del Magdalena se han realizado dos estudios. Para los tres sitios, uno se encuentra publicado y el otro no. De los no publicados, dos corresponden a trabajos de grado (Urabá y Magdalena) y el otro a un informe técnico (San Andrés). Para La Guajira, hay un artículo que menciona algunos aspectos sobre las poblaciones de este cangrejo. Para el departamento de Córdoba se tiene registro de dos tesis de grado. Para el departamento del Atlántico hay un informe técnico. Los departamentos Bolívar y Antioquia tienen tres estudios cada uno. La mayoría de estas investigaciones contienen información sobre las tallas y los pesos de los cangrejos, y en algunos también es posible evidenciar información sobre la densidad poblacional a través del conteo de madrigueras. En la isla de San Andrés se han registrado las mayores tallas y pesos en promedio. Las mayores densidades poblacionales promedio se han registrado para Bahía Concha en el departamento del Magdalena y las menores para el departamento de Bolívar en el Corregimiento de La Boquilla.

Abstract The blue land crab Cardisoma guanhumi is a species of commercial interest and is being harvested for human consumption. It is distributed throughout the Caribbean from the Antilles to the southern part in Venezuela and Colombia, with also records in Brazil. The first studies of this crab in Colombia date back to the early 1970s, which focused on biological and ecological aspects. Population studies in the Colombian Caribbean started in 2000, but they are scarce, and some are not published. For San Andrés Island, the Gulf of Urabá, and the Magdalena department, two studies have been done for each one, and for the three studies one is published, and the other is not. Of the unpublished studies, two correspond to graduate studies (Urabá and Magdalena) and one to a technical report (San Andrés). For the Guajira department, there is a published article that mentions some aspects of this crab population. For the department of Atlántico, there is a technical report. Regarding the department of Córdoba, there are two theses. The departments of Bolivar and Antioquia have three studies each. Most of these studies contain information about the sizes and weights of the blue land crab, and some of them also have information on the population density via burrow count. The largest average sizes and weights have been recorded on the island of San Andrés. The highest average population densities have been recorded for Bahía Concha in the department of Magdalena and the lowest for the department of Bolívar in the Corregimiento La Boquilla.

ABSTRACT The aim of this study was to characterize the microplastics (MP) in the hairy crab Romaleon setosum (Molina, 1782) (Cancridae), a species of importance for human consumption. In total, 15 individuals of R. setosum acquired between December 2020 and January 2021 at the Ventanilla Fisheries Wholesale Market, Callao, Peru were evaluated. The muscle, gill and digestive tract of each crab was digested with 10% KOH, followed by a filtration process to search for MP. All individuals presented MP in muscle, gills and digestive tract. Blue MP fibres were most abundant in muscle, blue and red fibres in the gill, and black and blue in the digestive tract. In the case of film-type PM, white was the dominant colour in the muscle and digestive tract, while green was observed in the gills. For the fragments, the blue colour was the most abundant in the gill and in the digestive tract, not being observed in the muscle. The number of PM particles were present with the following sequence: digestive tract > gill > muscle and in relation to its shape it was fibre > fragment > film. The blue and black MPs were the dominant ones. The length of the cephalothorax was related to the number of total PM particles, to muscle and to the blue colour.

RESUMEN El objetivo de esta investigación fue caracterizar los microplásticos (MP) en el cangrejo peludo Romaleon setosum (Molina, 1782) (Cancridae), especie de importancia para el consumo humano. Se evaluaron 15 individuos de R. setosum adquiridos entre diciembre de 2020 y enero de 2021 en el Mercado Mayorista Pesquero de Ventanilla, Callao, Perú. El músculo, branquia y tracto digestivo de cada cangrejo fue sometido a digestión con KOH al 10%, seguido por un proceso de filtración para la búsqueda de los MP. Todos los individuos presentaron MP en músculo, branquias y tracto digestivo. Las fibras azules de MP fueron más abundantes en el músculo, las azules y rojas en la branquia, y el negro y azul en el tracto digestivo. En el caso del MP tipo film, el color blanco fue el dominante en el músculo y en el tracto digestivo, en tanto que el verde se observó en las branquias. Para los fragmentos, el color azul fue el más abundante en la branquia y en el tracto digestivo, no siendo observado en el músculo. El número de partículas de MP se presentó con la siguiente secuencia: tracto digestivo > branquia > músculo y con relación a su forma fue fibra > fragmento > film. Los MP de color azul y negro fueron los dominantes. La longitud del cefalotórax se observó relacionada con el número de partículas de MP totales, con el músculo y con el color azul.

Abstract The blue swimming crab (Portunus pelagicus (Linnaeus, 1758)) is an economically and ecologically important species in Vietnam, and a potential subject for aquaculture as well. More than 400 individual crabs were collected along the Vietnamese coastline and examined for ectosymbiotic crustaceans. Two molecular markers (28S rRNA and COI mtDNA) were applied for species delineation. Seven species were reported and described, of which six are cirripede barnacles (Thecostraca, Thoracia); and one parasitic copepod Choniosphaera indica Gnanamuthu, 1954 (Copepoda, Podoplea). Four species (Chelonibia testudinaria (Linnaeus, 1758), Semibalanus sp., Octolasmis neptuni (MacDonald, 1869), and Dianajonesia tridens (Aurivillius, 1894)) were the first records for both host and location. The symbiotic crustaceans occupy specific niches on the crab body, and vary in their infestation status. Molecular taxonomy of symbiotic crustaceans was classified and confirmed based on sequence similarity and phylogenetic analyses.

Abstract From January to June 2021, mark-recapture and biometric data were collected from a population of blue crab, Cardisoma crassum Smith, 1870, from Ponuga, in Veraguas, Panama. Recapture histories of 163 crabs indicate strong burrow fidelity. During the dry season (January-March) burrow fidelity was 73.2 %, while in the rainy season (April-June) it was 50.7 %. Population was estimated at 2,569 crabs in 10.93-ha with a density of 0.024 ind. m-2. Cephalothorax width averaged 63.62 ± 6.37 mm for males (50.95-78.01 mm, N = 83) and 61.90 ± 5.47 mm for females (53.21-77.78 mm, N = 80). Blue crab reproductive season was traditionally known to take place just during the rainy season. Here, we report ovigerous females in February and March (dry season), suggesting reproduction may occur throughout the year in Panama. This is the first report of burrow fidelity and continuous biometry measurements in natural conditions for C. crassum. Burrow fidelity seems to be closely related to abiotic factors and is different between dry and rainy seasons.

ABSTRACT Background: The Blue crab Callinectes sapidus (Rathbun, 1896) is an important fishery in socio-economic terms for the Mexican waters of the Gulf of Mexico. Despite its relevance, the blue crab has been little studied. Fisheries management generally involves decision-making based on the assessment of population size and dynamics. Goals: Determine the level of exploitation of the Blue crab resource in the Gulf of Mexico using official catch data from 1980 to 2018. Methods: The Catch-Maximum Sustainable Yield (C-MSY) method allows estimation of reference points that can be used for management of a fishery, including Catch at Maximum Sustainable Yield (MSY), Biomass associated with MSY (BMSY) and Mortality associated with MSY (FMSY) using limited data. Results: The values of MSY and BMSY found by the C-MSY method were 16,491 t and 27,687 t, respectively. The FMSY value was 0.596, with a k value of 55,374 t, varying between 33,842 and 90,604 t. The highest viable r value of maximum net productivity was equal to 1.19. Conclusions: The results indicate that the blue crab (C. sapidus) fishery in the Gulf of Mexico is at healthy levels, but wich maximum levels of exploitation. To maintain the stock status, it is recommended that catch do not exceed the 14,842 t (0.9 MSY) and/or fishing mortality equal to 0.9 FMSY.

RESUMEN Antecedentes: La jaiba azul Callinectes sapidus (Rathbun, 1896) es una pesquería importante en términos socioeconómicos para el Golfo de Mexico. A pesar de su relevancia la jaiba azul ha sido poco estudiada. El manejo pesquero generalmente implica la toma de decisiones basadas en la evaluación del tamaño y la dinámica de la población. Objetivos: Determinar el nivel de explotación de la jaiba azul del Golfo de México utilizando datos oficiales de captura de 1980 a 2018. Métodos. El método de rendimiento máximo sostenible de captura (C-RMS) permite estimar los puntos de referencia que pueden utilizarse para la gestión de una pesquería, incluidas las capturas con rendimiento máximo sostenible (RMS), la biomasa asociada al RMS (BMSY) y la mortalidad asociada al RMS (FMSY) utilizando datos limitados. Resultados: Los valores de RMS y BMSY encontrados por el método C-MSY fueron 16,491 y 27,687 t, respectivamente. El valor de FMSY fue de 0.596, con un valor k de 55,374 t, variando entre 33,842 y 90,604 t. El valor r más alto viable de la productividad neta máxima fue igual a 1.19. Conclusiones: Los resultados indican que la pesquería de jaiba azul (C. sapidus) en el Golfo de México se encuentra en niveles saludables, pero con niveles máximos de explotación. Para mantener la situación de la población, se recomienda que la captura no exceda las 14,842 t (0.9 RMS) y/o una mortalidad por pesca igual a 0.9 FMSY.

ABSTRACT Eyestalk ablation allows the removal of neurohormones that inhibit early reproduction and decreases the time to complete the molting cycle. The present study evaluated the impact of unilateral eyestalk ablation on the behavior of blue crab breeders Callinectes arcuatus in the short term under laboratory conditions. Immature male and female crabs and individuals in the intermolt stage were collected in Ohuira Bay, Ahome, Sinaloa, Mexico. Three bioassays were performed: males, females, and male-females each with three treatments; control + xylocaine (50 μL, 5%), squash ablation + xylocaine (50 μL, 5%), ablation by string ligation + xylocaine (50 μL, 5%), each with three replicas and three periods of evaluation after the ablation procedure (10 min, 2 h, and 24 h). Kruskal-Wallis and Chi-square (χ2) tests were used to evaluate significances (P < 0.05) in the behavior of C. arcuatus. The sizes of blue crab C. arcuatus were 9.72 ± 1.0 cm of carapace width. The feeding behavior of the male-females bioassay was significant at 2 h (P < 0.05). The results obtained were satisfactory using two techniques, unilateral eyestalk ablation by squash and ligation in which pain and aggressiveness indicators were mitigated. Disorientation and aggressiveness in males were significant at 2 h (P < 0.05), and in females after 10 min, and 2 h after ablation (P < 0.05). In the male-females bioassay, aggressiveness was significant at 2 h (P < 0.05). Courtship and copulation were recorded in those crabs subjected to unilateral eyestalk squash ablation (13 and 8, respectively).

Abstract: The selectivity of two types of commercial fishing gear (crab hoop/traditional gear and rigid rectangular cage traps) targeting blue crabs (Callinectes arcuatus) in Cuyutlan Lagoon, Mexico, was assessed. The effect of soak time on crab catches versus the retention and escape rate of organisms was also evaluated. Results suggested that traps captured larger individuals than hoops. The carapace width of crabs was significantly different between the fishing gears used: 63.9% of crabs caught with hoops were < 95 mm (sizes ranged from 40 to 113 mm) and 40.7% of crabs caught with traps were < 95 mm (range: 49 to 120 mm). Results also showed that catch rates were high during the first two hours and decreased after the third hour. The crabs tended to escape after several hours of soak time. The study found that the traps captured larger crabs than the hoops, suggesting that traps are a suitable fishing gear for catching blue swimming crabs in Cuyutlan Lagoon.

Resumen: Se analizó la selectividad de dos tipos de artes de pesca comercial (aros jaibero/arte tradicional y trampas rectangulares de material rígido) que se utilizan para capturar la jaiba azul (Callinectes arcuatus) en la pesquería que opera en la Laguna de Cuyutlán, México. Asimismo, se analizó el efecto del tiempo de remojo de las trampas sobre las tasas de captura y de escape de los organismos. Los resultados del primer experimento sugirieron que la trampa rectangular capturó organismos más grandes, en comparación con el aro jaibero. La estructura del ancho de caparazón fue significativamente diferente entre los artes de pesca evaluados: los aros retuvieron el 63,9% de tallas < 95 mm (intervalos entre 40 y 113 mm) y las trampas el 40,7% de tallas < 95 mm (intervalos entre 49 y 120 mm). Los resultados del segundo experimento mostraron que la tasa de captura fue mayor durante las primeras dos horas del tiempo de remojo, y después de la tercera hora la tasa de captura disminuyó. Después de varias horas de tiempo de remojo, los organismos tendieron a escapar. Se encontró que la trampa captura organismos de jaiba azul más grandes que el aro, por lo que es un arte más adecuado en la Laguna de Cuyutlán, México.

ABSTRACT In the Alvarado Lagoon System (ALS) in south-central Veracruz on Mexico's gulf coast, the blue land crab, Cardisoma guanhumi, a semi-terrestrial crustacean species, represents an important fishery resource, but one that is subject to overexploitation and habitat destruction. Identifying genetic diversity and the genetic unit are required to establish efficient conservation strategies. The present study's goal was to assess the genetic structure and degree of genetic diversity of C. guanhumi populations along the ALS. A fragment of the D-loop region of the mtDNA (750 bp) and a partial sequence of the COI gene (580 pb) was sequenced for 120 specimens collected at four sites in the ALS. Genetic diversity indices were estimated for each marker. The study sites’ gene flow was estimated, as was the effective population size and the mutational rate for each locality. A haplotype network was built to estimate the gene genealogies of the populations. A Tajima's neutrality test, Fu tests, and mismatch analysis were performed to detect demographic expansion. Results show that this C. guanhumi population has high genetic diversity with a large gene flow among the localities studied and expanded during the recent evolutionary past. All haplotypes from both markers are novel for the species, according to the NCBI GenBank. The high genetic diversity and genetic connectivity among sites suggests that the size of blue crab populations in the ALS has not been drastically affected by past exploitation. Finally, management strategies are proposed to protect the species.

ABSTRACT The relationship between color and size supports the hypothesis that color is an important signal for some crab species during mating rituals or agonistic interactions. Thus, the objective of this study was to describe the color variation of D. pagei and its relationship with sex and sexual maturity. The crabs were placed on a surface for photographic recording of the body parts: cephalothorax, chelipeds, abdomen and pereiopods using a digital camera installed on a photographic table. Images were converted to the spectrum of red, green, blue and also of them combined (RGB). The values of each spectrum revealed that the color variation of the cephalothorax and chelipeds were different between adults and juveniles. The abdomen and pereiopods showed color variation in both sexes and at developmental stages. The pattern of color variation observed in juveniles ranged from light to dark brown and adults are from orange to intense red. The change in color of the cephalothorax and chelipeds could be used as a character to determine the onset of sexual maturity. The high color variation of the abdomen and pereiopods in both sexes and at developmental stages, though, indicates individual variation which probably is associated with other factors, such as molt stage or even the quality of food available.

RESUMEN En este estudio se describe la abundancia, composición por tallas y crecimiento relativo de organismos juveniles de la jaiba Callinectes sapidus en el estuario ciego Laguna El Llano, Veracruz, México; las jaibas se muestrearon desde mayo de 2013 hasta mayo de 2014 en ocho sitios, cuatro sin vegetación, con sedimentos arenosos y aguas claras cercanos a la boca estuarina, los restantes con vegetación de manglar, sedimentos lodosos y aguas turbias. Se registraron las variables hidrológicas pH, temperatura, oxígeno disuelto, turbidez y salinidad. Se recolectaron 276 jaibas (235 machos y 41 hembras), la abundancia fue mayor (91,3 %) en los sitios cercanos a manglares, sedimentos lodosos y aguas turbias; la abundancia no se relacionó con las variables hidrológicas (P > 0,05). Por individuo se midió el ancho de caparazón (AC) y peso (P); los machos se registraron en un intervalo de AC entre 12 y 85 mm y de 0,3 a 123,4 g, las hembras se recolectaron entre 9 y 92,8 mm AC y pesaron entre 0,7 a 152,6 g; 76,4 % de los especímenes correspondieron a tallas de 11 a 40 mm AC; se analizó el crecimiento relativo usando la relación biométrica AC vs. P, que para las jaibas jóvenes fue alométrica negativa independientemente del sexo; todos los individuos presentaron el telson diferenciado pero en etapas iniciales de desarrollo gonádico, la proporción sexual fue dominada por los machos; la ocurrencia de la talla de 11 a 20 mm AC durante el estudio indicó reclutamiento continuo al estuario.

ABSTRACT In this report are described abundance, size structure, and relative growth in Callinectes sapidus young stages from the Laguna El Llano blind estuary, Veracruz State (Mexico). Blue crabs were sampled in eight sampling sites since May 2013 to May 2014; four sites were located in un-vegetated, sandy bottom, and clear waters locations near to estuarine inlet, another four with fringe mangrove vegetation, muddy-bottom, and turbid waters. Were recorded pH, temperature, dissolved oxygen, turbidity, and salinity. A total of 276 organisms were collected (235 males and 41 females), young blue crabs were most numerous (91.3 %) in muddy-bottom, turbid waters sampling sites; the temporal abundance was not related statistically to hydrological variables (P >0.05). Were measured the Carapace Width (CW) and total weight (W) of each specimen, male CW ranked 12-85 mm and WW 0.3-123.4 g, while female CW was 9.0-92.8 mm and W 0.7-152.6 g; respect to total abundance, 76.4 % were 11-20 mm and 4160 mm CW size class specimens; the relative growth was analyzed by using CW vs. W biometric relationship, for young blue crabs were negative allometric growth, regardless of sex; all specimens showed morphologically differentiated telson but early gonadal development stages; males outnumbered the gender proportion; 11-20 mm CW blue crabs occurrence through study span indicated continuous recruitment processes in this estuary.

RESUMEN En Colombia, el cangrejo Cardisoma guanhumi enfrenta dos amenazas: la extracción y la pérdida de hábitat. El presente trabajo tuvo como objetivo estimar los parámetros de crecimiento de C. guanhumi en el departamento del Magdalena. Se realizaron capturas mensuales de mayo a octubre de 2016 en cuatro localidades del Parque Nacional Natural Tayrona (PNNT) y una localidad de la Vía Parque Isla Salamanca (VIPIS). Se capturaron 816 individuos, los cuales fueron medidos, pesados, sexados y liberados. Con la rutina ELEFAN I del programa FiSAT II se analizaron las frecuencias de tallas obtenidas para conocer: 1) los parámetros de crecimiento a nivel departamental, teniendo en cuenta el sexo, para lo cual se usaron todos los datos y 2) para el PNNT, para lo cual se excluyeron los datos de VIPIS. El tamaño asintótico del ancho del caparazón fue mayor en machos (109.16 mm) que en hembras (96.74 mm), mientras que el parámetro de crecimiento K fue mayor para las hembras (0.19 año-1) que para los machos (0.15 año-1). Se obtuvieron índices de desempeño de crecimiento similares: 3.252 en machos y 3.250 en hembras. Con la rutina de curva de captura linealizada de FISAT II se calcularon las tasas de mortalidad total (Z), natural (M), y por pesca (F), las cuales fueron 0.41, 0.36 y 0.05 año-1 respectivamente. Para el PNNT se obtuvo un K mayor (0.29 año-1) que en el departamento (0.15 año-1) y por tanto una menor longevidad (tmax = 10.42 años) que en el departamento (tmax = 20.06 años). En términos generales la población de C. guanhumi es longeva, de crecimiento lento y con baja mortalidad por extracción en la zona de estudio. Los datos obtenidos en el presente trabajo son importantes para el manejo de esta especie, ya que al tener un crecimiento lento la hace vulnerable a la sobrepesca.

ABSTRACT In Colombia, the blue land crab Cardisoma guanhumi faces two main threats: extraction and habitat loss. This project aims to show an approximation of the growth parameters of C. guanhumi in protected areas within the Magdalena department. Individuals were captured monthly from May to October 2016 in five localities in the Tayrona National Natural Park (PNNT) and the Salamanca Island Road Park (VIPIS). A total of 816 crabs were collected, each of which was measured, weighed, sexed and released. Both sexes showed a negative allometric length-to-weight ratio. Using the FiSAT II program's ELEFAN I routine, the size frequencies obtained were analyzed in order to determine the growth parameters for 1) the department, considering gender, and 2) the PNNT, where the data from VIPIS was excluded. The asymptotic carapace width was higher for males (109.16 mm) than for females (96.74 mm), but the growth coefficient (K) was higher for females (0.19 year-1) than for males (0.15 year-1). Growth performance indexes were similar: 3.252 for males and 3.250 for females. The FISAT II length converted catch curve routine was used to calculate the total (Z), natural (M), and fishing (F) mortality rates, as 0.41, 0.36, and 0.05 year-1 respectively. The grow coefficient K was higher for the PNNT (0.29 year-1) than for the Magdalena department (0.15 year-1), thus, longevity was lower for the PNNT (tmax = 10.42 years) compared to the department (tmax = 20.06 years). In general terms, the C. guanhumi population in the study area is longevous, slow growing, with low extraction morality. The data obtained in this work are important in the management of this species, as its slow growth makes it vulnerable to overfishing.

In this scientific work, it was developed a single analytical procedure for the sample preparation of different tissues of blue crabs using microwave-assisted digestion for the determination of Cu, Mn, Fe and Zn by fast sequential flame atomic absorption spectrometry (FS FAAS). The limits of quatification were 10.5, 1.6, 8.9 and 19.3 µg g-1 for Cu, Mn, Fe and Zn, respectively. The precision was estimated in terms of repeatability using the relative standard deviation (RSD, n = 7) 3.4% for Cu; 4.8% (Mn); 4.7% (Fe) and 4.7% (Zn) and accuracy of the procedure was confirmed by analysis of certified reference material (CRM) of oyster tissue (NIST 1566B) with concentration values of 71.3 for Cu, 18.3 for Mn, 202.5 for Fe and 1415 µg g-1 for Zn. The procedure has been applied for the determination of Cu, Mn, Fe and Zn in several blue crab samples from Cachoeira River estuary, Ilhéus, Bahia, Brazil.