BACKGROUND: Collaboration between traditional health practitioners (THPs) and biomedical health practitioners (BHPs) is highly recommended in catering for pluralistic healthcare users. Little is known about bidirectional collaborations at healthcare service provision level. AIM: To map global evidence on collaboration attempts between THPs and BHPs between January 1978 and August 2023 METHOD: We followed the Arksey and O'Malley framework in conducting this scoping review. Two reviewers independently screened articles for eligibility. A descriptive numerical and content analysis was performed on ATLAS.ti 22. A narrative summary of the findings was reported using the PRISMAScR guideline. RESULTS: Of the 8404 screened studies, 10 studies from 12 articles were included in the final review. Studies came from America (n = 5), Africa (n = 2), China (n = 2) and New Zealand (n = 1). Eight studies reported case studies of bidirectional collaboration programmes, while two studies reported on experimental research. All collaborations occurred within biomedical healthcare facilities. Collaboration often entailed activities such as relationship building, training of all practitioners, coordinated meetings, cross-referrals, treatment plan discussions and joint health promotion activities. CONCLUSION: This study confirmed that practitioner-level collaborations within healthcare are few and sparse. More work is needed to move policy on integration of the two systems into implementation. There is a need to conduct more research and document emerging collaborations. CONTRIBUTION: This research illuminates the contextual challenges associated with sustaining collaborations. The data would be important in informing areas that need strengthening in the work towards integration of THPs and BHPs.

Abstract Background: Excitotoxicity-induced in vivo injury models are vital to reflect the pathophysiological features of acute spinal cord injury (SCI) in humans. The duration and concentration of chemical treatment controls the extent of neuronal cell damage. The extent of injury is explained in relation to locomotor and behavioural activity. Several SCI in vivo methods have been reported and studied extensively, particularly contusion, compression, and transection models. These models depict similar pathophysiology to that in humans but are extremely expensive (contusion) and require expertise (compression). Chemical excitotoxicity-induced SCI models are simple and easy while producing similar clinical manifestations. The kainic acid (KA) excitotoxicity model is a convenient, low-cost, and highly reproducible animal model of SCI in the laboratory. The basic impactor approximately cost between 10,000 and 20,000 USD, while the kainic acid only cost between 300 and 500 USD, which is quite cheap as compared to traditional SCI method. Methods: In this study, 0.05 mM KA was administered at dose of 10 μL/100 g body weight, at a rate of 10 μL/min, to induce spinal injury by intra-spinal injection between the T12 and T13 thoracic vertebrae. In this protocol, detailed description of a dorsal laminectomy was explained to expose the spinal cord, following intra-spinal kainic acid administration at desired location. The dose, rate and technique to administer kainic acid were explained extensively to reflect a successful paraplegia and spinal cord injury in rats. The postoperative care and complication post injury of paraplegic laboratory animals were also explained, and necessary requirements to overcome these complications were also described to help researcher. Results: This injury model produced impaired hind limb locomotor function with mild seizure. Hence this protocol will help researchers to induce spinal cord injury in laboratories at extremely low cost and also will help to determine the necessary supplies, methods for producing SCI in rats and treatments designed to mitigate post-injury impairment. Conclusions: Kainic acid intra-spinal injection at the concentration of 0.05 mM, and rate 10 μL/min, is an effective method create spinal injury in rats, however more potent concentrations of kainic acid need to be studied in order to create severe spinal injuries.

RESUMO A berinjela é uma cultura hortícola muito valorizada, cultivada em todo o globo e de grande importância econômica na Ásia. No entanto, sua produção está ameaçada por várias doenças foliares ou transmitidas pelo solo, pragas, seca, calor e danos causados por geada. Portanto, esforços são importantes para transferir genes de resistência para a berinjela, provenientes de seus parentes selvagens. No presente estudo, foi realizada hibridização interespecífica e intraespecífica, que incluiu três genótipos cultivados de berinjela (Solanum melongena MEE, Solanum melongena MEP, Solanum melongena MEB) e uma espécie selvagem de Solanum (Solanum incanum INC). Os híbridos F1 foram produzidos por hibridização inter e intraespecífica. Um total de 632 cruzamentos recíprocos inter e intraespecíficos possíveis foram realizados, onde apenas três tiveram sucesso. Os dias mínimos para o florescimento foram observados na MEP parental, e a altura máxima da planta foi medida em MEE×MEB. O comprimento máximo do fruto foi observado na MEB parental. Além disso, o diâmetro do fruto, a largura da folha, o comprimento da folha e a produção de frutos por planta foram encontrados máximos no híbrido MEExINC. Nossos resultados sugerem que esses materiais serão de grande interesse para o melhoramento genético da berinjela; eles podem ter enorme potencial para aumentar a tolerância aos estresses abióticos, como seca e calor, bem como aumentar os valores nutricionais. Os resultados encontrados neste estudo serão úteis para a saúde humana, em última análise, contribuindo para o desenvolvimento de uma nova geração de plantas adaptadas ao clima.

ABSTRACT The eggplant is a highly valuable horticultural crop grown all over the world and it is of substantial economic importance in Asia. However, its production is severely threatened by several soil-borne and foliar diseases, insect-pests, drought, heat, and frost damage. Therefore, efforts to transfer useful resistance genes into eggplant from their wild relatives is important. In the present study, interspecific and intraspecific hybridization was carried out, that included three cultivated genotypes of eggplant (Solanum melongena MEE, Solanum melongena MEP, Solanum melongena MEB) and one wild Solanum species (Solanum incanum INC). The F1 hybrids were made by inter and intraspecific hybridization. A total of 632 possible inter and intraspecific reciprocal crosses was performed where only three were successful. The minimum days to flowering were observed in parent MEP, and maximum plant height was measured in MEE×MEB. Maximum fruit length was observed in parent MEB. Furthermore, fruit diameter, leaf width, leaf length, and fruit yield per plant were found maximum in hybrid MEExINC. Our results suggest that these materials will be of great interest for the genetic improvement of eggplant; they may have a tremendous potential to increase tolerance to abiotic stresses, such as to drought and heat, as well as increased nutrient and herbal values. Findings of this study will be helpful for the human health, ultimately contributing to the development of a new generation of plants adapted to climate.