Helicobacter pylori is a bacterium recognized as the major cause of chronic gastritis and peptic ulcers. Infection by H. pylori induces inflammatory responses and pathological changes in the gastric microenvironment. The host Keywords: immune cells (especially neutrophils) release inflammatory mediators and large 5-methoxy-3,4-dehydroxanthomegnin amounts of reactive oxygen species (ROS), which are associated with an increased Helicobacter pyloririsk of developing gastric cancer. In this study, we evaluated the anti-H. pylori and oxidative burst antioxidantactivitiesofa1,4-naphthoquinone-5-methoxy-3,4-dehydroxanthomegnin. Paepalanthus latipes The antimicrobial activity was assessed using a spectrophotometric microdilution technique, and antioxidant activity was assessed by noting the effect of 5-methoxy3,4-dehydroxanthomegnin on the neutrophil oxidative burst using luminol-and lucigenin-amplified chemiluminescence. The results showed that 5-methoxy-3,4dehydroxanthomegnin is a potent anti-H. pylori compound (MIC 64 µg/mL and MBC 128 µg/mL) and a strong antioxidant. 5-Methoxy-3,4-dehydroxanthomegnin decreased luminol- and lucigenin-amplified chemiluminescence, with ED50 values of 1.58±0.09 µg/mL and 5.4±0.15 µg/mL, respectively, reflecting an inhibitory effect on the oxidative burst. These results indicate that 5-methoxy-3,4-dehydroxanthomegnin is a promising compound for the prevention and treatment of diseases caused by H. pylori infection, such as gastritis, peptic ulceration, and gastric cancer, because reactive oxygen intermediates are involved in the pathogenesis of gastric mucosal injury induced by H. pylori infections.

Large number of quinones has been associated with antitumor, antibacterial, antimalarial and antifungal activities. In this work we describe the effect of the naphthoquinone, 5-methoxy-3,4-dehydroxanthomegnin, on murine tumor cells (LP07 and LM2) and its immunomodulatory effect on nitric oxide (NO) production on LPS-stimulated macrophages. The results have shown that 5-methoxy-3,4-dehydroxanthomegnin was a significant inhibitor of LPS-stimulated NO generation from macrophage (inhibition percentage ranged from 97.4 to 98.9%) and a strong cytotoxic agent against both tumor cells LP07 and LM2 (CI50 6.2±0.36 µM and 74.6±1.9 µM, respectively). These results indicate that the 5-methoxy-3,4-dehydroxanthomegnin may show promising activity in the treatment of murine breast and lung cancer by immunomodulatory and antiproliferative activities.

Com o objetivo de caracterizar a influência da ocupação na qualidade do ar interior, um edifício público com sistema de ar condicionado foi selecionado. As variáveis ambientais consideradas incluíram contagem total de bactérias e fungos, temperatura, umidade relativa e concentração de dióxido de carbono. O número de ocupantes, que variou durante todo o dia, foi estimado em cada amostragem. As amostras foram coletadas antes do início do expediente de trabalho e durante 3 horas, em intervalos de 30 minutos, por 5 dias úteis consecutivos. A análise de correlação demonstrou que a taxa de ocupação foi correlacionada positivamente com a concentração de bactérias, dióxido de carbono e temperatura. Nenhuma associação significativa foi observada entre o número de ocupantes e concentração de fungos. Os resultados deste estudo fornecem informações quanto à variabilidade nos parâmetros do ar interior no decorrer do dia em um edifício onde a ocupação é dinâmica.

To characterize the influence of occupancy on the indoor air quality, a public office building with air-conditioning system was selected for this study. The indoor parameters included total bacteria count, total fungal count, temperature, relative humidity, and carbon dioxide concentration. The number of occupants, which varied throughout the day, was recorded in each sample. The samples were taken before the beginning of the working day and during 3 h, at an interval of 30 min between each sampling, and continued for five working days during a week. Correlation analysis demonstrated that occupancy rates were positively correlated with airborne bacteria, CO2, and temperature. No significant association between the number of occupants and fungus was observed. The results of this study provided information on the variability of indoor air parameters during the time-varying occupancy over the course of the day in at air-conditioned buildings where occupancy was quite dynamic.

Foram colhidas amostras de mãos e fossas nasais de 48 manipuladores de alimentos das principais casas comerciais da cidade de Araraquara, Estado de São Paulo (Brasil), e de 20 estudantes universitários. Dentre os indivíduos foram encontrados 44,1% e 34,8% que portavam Staphylococcus aureus em fossas nasais e mãos, respectivamente. Observou-se predomínio de fagotipos dos grupos I e III. Dos 12 portadores do microrganismo, concomitantemente em mãos e fossas nasais, 75,0% apresentaram cepas com vínculo epidemiológico. Os achados mostram o risco potencial representado pelas mãos nas intoxicações alimentares.

Material was collected from the hands and nasal passages of forty-eight food handlers and twenty college students of Araraquara (S. Paulo State, Brazil) and analized in order to evaluate the carrier function with regard to Staphylococcus aureus. The organism discovered in both samples of nine out of the twelve volunteers were of the same S. aureus phage types. The incidence of carriage on the hands was much greater in the handlers' group. These findings demonstrate the potential risk represented by hands in the transmission of food poisoning.

Foram colhidas amostras de mãos e fossas nasais de 48 manipuladores de alimentos das principais casas comerciais da cidade de Araraquara, Estado de São Paulo (Brasil), e de 20 estudantes universitários. Dentre os indivíduos foram encontrados 44,1% e 34,8% que portavam Staphylococcus aureus em fossas nasais e mãos, respectivamente. Observou-se predomínio de fagotipos dos grupos I e III. Dos 12 portadores do microrganismo, concomitantemente em mãos e fossas nasais, 75,0% apresentaram cepas com vínculo epidemiológico. Os achados mostram o risco potencial representado pelas mãos nas intoxicações alimentares.

Material was collected from the hands and nasal passages of forty-eight food handlers and twenty college students of Araraquara (S. Paulo State, Brazil) and analized in order to evaluate the carrier function with regard to Staphylococcus aureus. The organism discovered in both samples of nine out of the twelve volunteers were of the same S. aureus phage types. The incidence of carriage on the hands was much greater in the handlers' group. These findings demonstrate the potential risk represented by hands in the transmission of food poisoning.