Abstract The aim of this study was to develop and analyze the psychometric properties of the Brazilian Version of Lay Person's Social Judgement about Cleft-lip Scale (B-LSojCleft-S). A standardized photograph of a 16-year-old girl was manipulated by using photo editing software, to simulate different cleft-lip situations. The cleft-free image was used as a control. The researchers structured an initial questionnaire that was evaluated by experts and a sample of the target population to evaluate the construct. After analysis by the researchers, a final version of the B-LSojCleft-S containing 14 items was generated. Each answer was awarded score from 1 to 3 points, yielding a total score of 14 to 42 points. Higher scores represented better social judgements. The B-LSojCleft-S was applied online to 103 test participants and 73 retest participants with an interval of 20 days between application. Aspects of acceptability, discriminant property, internal consistency, reliability and construct validity were evaluated. Construct validity was assessed using the Friedman test (p < 0.05). Reliability was determined using an intraclass correlation coefficient > 0.70, and internal consistency using Cronbach's alpha > 0.81. The B-LSojCleft-S showed high acceptability, strong discriminant property, excellent internal consistency and reliability, but had a floor and ceiling effect. The instrument reached valid and reliable scores and had acceptable psychometric properties to evaluate the social judgments of lay persons about different cleft lip situations in a Brazilian population.

ABSTRACT Objective: To evaluate the presence of sequelae in primary teeth and their permanent successors, as well as associations between TDI and the presence of sequelae in both teeth. Material and Methods: In this 14-year retrospective study, 2.290 records were reviewed, 192 patients who suffered dental trauma in primary teeth were followed until the eruption of successor teeth were included. Descriptive, chi-square, and regression logistic with generalized estimating equations tests were performed (p<0.05). Results: 362 primary and successor teeth were followed. Sequelae were present in 71.8% of primary teeth and 25.7% of their successors. Teeth with complicated fracture (100%), extrusion (100%) and avulsion (100%) resulted in the largest amounts of sequelae in primary teeth and intrusion (61%) on their successors. Age was associated with sequelae in permanent teeth (p<0.01). Extrusion (OR 10.06; CI 2.12-47.63) and intrusion (OR 7.51; CI 2.73-20.70) had a higher risk to cause sequelae in primary and permanent teeth, respectively. Conclusion: The type of injury involved influenced the sequelae in traumatized teeth and their successors, and the age of the child influenced the presence of sequelae in permanent teeth.

Abstract We compared sampling techniques in a managed native forest in Paragominas, Pará, Brazil. Our goal in this study was to evaluate the feasibility of using Double Sampling (DS) and Sampling with Partial Replacement (SPR), when compared to Continuous Forest Inventory (CFI), to estimate the wood stock for trees with DBH ≥ 20 cm in a managed forest. In our results, DS had the best volume prediction, generating a sampling error of 5.20% (11.48 m³ ha-1) on the second occasion 3.86% (8.78 m³ ha-1). The average volume increment, estimated for the forest in the monitored period (2014-2016) was 6.88 m³ ha-1, with a relative sampling error of 63.09%. Therefore, as an alternative and of low cost, we suggest using DS in successive forest inventories in monitoring areas of forest resources in the Brazilian Amazon.

Resumo A paquimeningite hipertrófica idiopática é uma causa rara de sintomas neurológicos apresentando mielopatia por compressão da medula espinhal. Relatamos um caso de paquimeningite com manifestação primária de tetraparesia após trauma de baixa energia e recorrência dos sintomas de mielopatia 5 anos após a cirurgia. A paciente, uma mulher de 19 anos, foi submetida a extensa investigação sem evidências de qualquer doença de base. Uma biópsia da meninge revelou processo inflamatório inespecífico com extensa fibrose da dura máter, também visualizado no período perioperatório. Esses achados, associados à exclusão de outras causas, sugerem o diagnóstico de paquimeningite hipertrófica idiopática.

Abstract Idiopathic hypertrophic pachymeningitis is rare cause of neurological symptoms with myelopathy due to spinal cord compression. We report a case of pachymeningitis, which was manifested primarily by tetraparesis after low-energy trauma and recurrence the myelopathy symptoms after 5 years of surgery. The patient, a 19-year-old woman, was subjected to extensive investigation without evidence of any underlying disease. A meningeal biopsy was performed and showed an unspecific inflammatory process with extensive fibrosis of the dura mater. These findings, associated with the exclusion of other causes, suggest idiopathic hypertrophic pachymeningitis.

Abstract The addition of organic compounds to fertilizers has shown positive effects on plant metabolism. This study aimed to evaluate the macronutrient accumulation and productivity of potato with the use of biofertilizer (BF) applied to the leaves. A 2×7 factorial plot with a plot subdivided in time and ten replications used two forms of fertilization: a chemical fertilizer with conventional NPK (nitrogen, phosphorus and potassium: control) and conventional fertilizer plus biofertilizers (BF); evaluations were performed 31, 41, 51, 61, 71, 81 and 91 days after planting (DAP) using the Jelly cultivar. The BF increased the maximum N, K, Ca and Mg accumulations in the leaves, especially N and K, with the period of greatest accumulation occurring between 62 and 66 DAP. The accumulations of N, P, K, Ca and Mg in the tubers accelerated from 71 DAP. At the end of the cycle (e.g., 91 DAP), the increases in the nutrient accumulations of N, P, K and Ca were between 30 and 64% higher for the BF application, the Mg accumulations doubled and the S accumulations exhibited no differences between the evaluation periods. For the leaves, the following decreasing sequence of maximum accumulation was observed: K>N>Ca>Mg>S>P. For the tubers, the following decreasing sequence was obtained: K>N>P>Ca>Mg>S. The use of biofertilizers caused higher productivity of tubers of greater caliber and soluble solids contents in the cv. Jelly potato tubers.

Resumen La adición de compuestos orgánicos en fertilizantes ha mostrado un efecto positivo en el metabolismo de las plantas. El objetivo fue evaluar la acumulación de macronutrientes en tubérculos de papa con el uso de biofertilizantes líquidos (BF), aplicados a través de la hoja. El diseño experimental fue factorial 2×7 con una división en el tiempo y diez repeticiones, consistió en dos formas de fertilización: química con NPK convencional (control) y convencionales más biofertilizantes (BF) y evaluaciones en 31; 41; 51; 61; 71; 81 y 91 días después de la siembra (DAS), utilizando el cultivar Jelly. BF favorece las acumulaciones máximas de N, K, Ca y Mg en las hojas, entre 8 y 37%, especialmente para N y K, con un período de mayor acumulación entre 62 y 66 DAP. La acumulación de N, P, K, Ca y Mg en los tubérculos se acelera desde 71 DAP. Al final del ciclo (91 DAP), el aumento en la acumulación de nutrientes N, P, K y Ca es entre 30 y 64% mayor en la aplicación de BF líquido, el Mg se acumula el doble y S no difiere entre los períodos de evaluación. Para las hojas, se obtuvo la siguiente secuencia decreciente de acumulación máxima: K>N>Ca>Mg>S>P. Para los tubérculos, se obtuvo K>N>P>Ca>Mg>S. El sou de biofertilizantes proporciona una mayor productividad de tubérculos de mayor calibre y contenido de sólidos solubles en el papa cv. Jelly.

RESUMO Objetivo: Analisar, sob o prisma do autorrelato de antecedentes e consequentes, como a pandemia da COVID-19 piora a saúde de homens residentes no Brasil. Métodos: Estudo qualitativo, realizado com 200 homens residentes em todas as regiões do Brasil, mediante a aplicação de instrumento semiestruturado, hospedado em plataforma on-line. Os dados foram analisados com o método do Discurso do Sujeito Coletivo e ancorados no referencial teórico do Materialismo Histórico Dialético. Resultados: A pandemia da COVID-19 piorou a saúde mental dos homens, porque aprimorou a história problemática pessoal, afetiva, familiar, ocupacional, disfuncional e/ou mórbida, e causa consequências de somatização psíquica, dissolução familiar, fim do relacionamento afetivo, conflitos conjugais, isolamento social, dificuldade financeira, vulnerabilidade da situação de trabalho e desgaste ocupacional, mudanças repentinas de comportamento, barreiras no acesso aos cuidados de saúde e experiências de morte e luto prejudicadas. Conclusões: É necessário fortalecer redes de apoio social, a fim de minimizar os impactos diretos e indiretos causados pela materialidade da pandemia para a saúde mental e as várias dimensões da vida afetadas.

ABSTRACT Objective: To analyze, from the perspective of self-report of antecedents and consequences, how the COVID-19 pandemic decrease the health of men living in Brazil. Methods: Qualitative study, conducted with 200 men living in all regions of Brazil through the application of a semi-structured instrument, hosted on an online platform. The data were analyzed with the Collective Subject Discourse method and anchored in the theoretical framework of Dialectical Historical Materialism. Results: The COVID-19 pandemic decrease men’s mental health because it worsened the history of personal, affective, family, occupational, dysfunctional and/or morbid problems, causing consequences of psychic somatization, family dissolution, end of affective relationship, marital conflicts, social isolation, financial difficulty, vulnerability of the work situation and occupational exhaustion, sudden changes in behavior, barriers in access to health care and impaired experiences of death and grief. Conclusions: Social support networks need to be strengthened in order to minimize the direct and indirect impacts caused by the pandemic materiality for mental health and the various dimensions of life affected.

Resumo Objetivos: revisar a literatura disponível sobre os aspectos gerais dainfecção por SARS-CoV-2. Métodos: trata-se de uma revisão narrativa de literatura realizada nos meses de março asetembro de 2020. Resultados: a COVID-19, causada pelo novo coronavírus ou SARS-CoV-2, cresce com efeitos devastadores em todo o mundo. A literetura descreve dados epidemiológicos e sobre grupos de riscos para mortalidade da doença, a qual apresenta uma alta velocidade de trans-missão. A prevenção é a forma mais eficaz de combate à doença, persistindo ausências de fortes evidências sobre o tratamento. Vacinas ainda não estão disponíveis A dexametasona é efetiva para redução da mortalidade nas formas graves. Conclusão: apesar dos grandes esforços, à medida que o número de casos confirmados aumenta, evidências sobre transmissão, incidência, evolução da doença, letalidade, efeitos e os desfechos permanecem limitados e sem grandes níveis de evidência. Estudos ainda são necessários sobre todos os aspectos da doença.

Abstract Objectives: to review the available literature on the general aspects of SARS-CoV-2 infec-tion. Methods: this is a narrative literature review carried out from March to September 2020. Results: COVID-19 caused by the new coronavirus or SARS-CoV-2, grows with devas-tating effects worldwide. The literature describes epidemiological data and mortality risk groups of the disease, which presents a high rate of transmission. Prevention is the most effective way to fight the disease, persisting the absence of strong evidence on the treatment. Vaccines are not yet available. Dexamethasone is effective in reducing mortality in severe forms. Conclusions: despite great efforts, as the number of confirmed cases increases, evidence on transmission, incidence, disease progression, lethality, effects and outcomes remain limited and without any high levels of evidence. Studies are still necessary for all aspects of the disease.

ABSTRACT The aim of this study was to evaluate the effect of controlled atmosphere storage on quality of native and cultivated yerba mate produced in Arvorezinha (RS) and São Mateus do Sul (PR) after 0, 3, 6 and 12 months of storage under ambient conditions, low oxygen (1.0 kPa) and high carbon dioxide (3.0 kPa) partial pressures. Total chlorophyll concentration reduced over the storage period regardless of the atmosphere condition, form of cultivation and production region. Total carotenoids, regardless of the form and place of cultivation, decreased until three months of storage under 1.0 kPa O2. Although, the O2 reduction to 1.0 kPa maintains greener color and higher chlorophyll concentration after 12 months of storage. High carbon dioxide partial pressures (3.0 kPa) in the storage chamber increases the polyphenol concentration until six months of storage. The raw material originated from São Mateus do Sul (PR) has higher chlorophyll concentration and greener color, resulting in greater storage potential.

Resumo Objetivos: revisar a literatura disponível sobre a COVID-19 e seus aspectos maternos e perinatais. Métodos: urna revisão narrativa de literatura foi realizada nos meses de março a setembro de 2020, utilizando os descritores, coronavirus, Covid 19, SARS-CoV-2, pregnancy, gravidity, pregnancy complications e pregnancy complications infectius. Foram incluidos todos os desenhos de estudo, revisões, recomendações e notas técnicas, sem distinção de idioma e que trouxessem a abordagem do novo coronavírus no cenárlo gestacional e perinatal. Resultados: a pandemia de COVID-19 vem apresentando efeitos devastadores, acometendo milhões depessoas e ceifando quase um milhão de vidas em todo o mundo. Inicialmente as gestantes não er am vistas como grupo de risco para a doença, porém na medida em que aumentou a proporção de mulheres acometidas durante o ciclo gravídico-puerperal diversos estudos foram publicados demostrando risco aumentado de complicações. Estudos brasileiros também alertaram para um número elevado de mortes maternas, associados à presença de comorbidades, mas, sobretudo, aos determinantes sociais da doença e a falhas graves da assistência. Conclusão: observou-se a necessidade de novos estudos com adequado delineamento de pesquisa, pois muitos estudos são apenas cartas ou pequenas séries de casos, além de recomendações de especialistas, sem o rigor científico necessário.

Abstract Objectives: reviewing the available literature on COVID-19 infection and the maternal and perinatal outcomes. Methods: this is a narrative review of the literature carried out from March to September 2020, usingthe MESH: coronavirus, Covid 19, SARS-CoV-2, pregnancy, gravidity, pregnancy complications and pregnancy complications infectius. All study designs, reviews, recommendations and technical notes were included, without distinction of language and that would bring the approach of the new coronavirus in the gestational and perinatal scenario. Results: the COVID-19 pandemic has had devastating effects, affecting millions of people and claiming almost a million lives worldwide. Initially, pregnant women were not seen as a risk group for the disease, however as the proportion of women affected during the pregnancy-puerperal cycle increased, several studies were published showing an increased risk of complications. Brazilian studies have also warned of a high number of maternal deaths, associated with the presence of comorbidities but, above all, with the social determinants of the disease and serious failures in care. Conclusion: the need for new studies with an adequate research design was observed, as many studies are only letters or small series of cases, in addition expert recommendations, without the necessary scientific rigor.

Abstract The objective of this work was to evaluate the relationship between factors associated with the COVID-19 pandemic and depression, anxiety, and stress (DAS) in dentists. Factors associated with the COVID-19 pandemic were evaluated using a questionnaire and scores of the Depression, Anxiety, and Stress Scales-21 were measured. The differences between the DAS scores based on the factors associated with the COVID-19 pandemic were tested through the successive application of multivariate analysis of variance (MANOVA, α = 0.05). After a sample size calculation, 998 participants with a mean age of 39.39 (± 11.69) years were included. The effect size indicated that changes in sleep quality (η2 = 0.161), eating habits (η2 = 0.057), and physical health (η2 = 0.051) were the ones that most negatively affected DAS scores. The highest DAS scores were observed in professionals who lived with someone at high-risk for COVID-19 (p < 0.001) and in those who did not engage in leisure activities during the pandemic (p < 0.001). Dentists who worked on the frontline against COVID-19 had higher scores of anxiety and stress (p = 0.029). The highest scores for anxiety, depression, and/or stress were seen in dentists living with someone at high-risk for COVID-19, who acts on the frontline, who does not practice in leisure activities during the pandemic, who completely changed eating habits, quality of sleep and physical health during the pandemic. In general, DAS levels of dentists were associated with factors related to the COVID-19 pandemic.

Resumo Este estudo teve como objetivo avaliar os efeitos de partidas consecutivas de futebol escolar com intervalos de 24 e 48 horas no estado de recuperação física de jogadores Sub-19. Foram avaliados 35 homens (17,6±1,1 anos) atletas escolares sub-19. Durante a competição, foram realizados três jogos de 70-min de duração. Dezessete atletas tiveram intervalo de 24h entre cada jogo (grupo GGG). Dezoito atletas tiveram intervalo de 48 h entre o 2º e o 3º jogo (grupo GG48hG). Antes de cada jogo foram medidas a Qualidade Total de Recuperação, altura do salto com contra movimento, velocidade no sprint de 10-m, e força máxima isométrica lombar máxima. A carga interna do jogo foi calculada pelo produto da percepção subjetiva do esforço da sessão e tempo de cada jogo. Houve uma redução de 22% na qualidade total de recuperação (p <0,001) e de 12% no desempenho de sprint de 10 m (p <0,001) antes da terceira partida no grupo GGG, enquanto o grupo GG48hG não apresentou alterações para as mesmas variáveis (p> 0,05). O salto com contra movimento diminuiu antes da segunda partida em ambos os grupos (GGG = 12% e GG48hG = 10%; p <0,001), sem diferença entre os grupos (p> 0,05). Em adição, ambos os grupos não apresentaram alterações na força isométrica e na carga interna ao longo dos jogos (p> 0,05). Apesar de não proporcionar uma completa recuperação muscular, 48 h de intervalo entre o segundo e o terceiro jogo parece ter minimizado o efeito de jogos consecutivos na redução do desempenho.

Abstract This study aimed to evaluate the effects of cumulative school soccer matches separated by 24-h or 48-h intervals on the recovery status of U-19 players. Thirty-four school athletes (17.6 ± 1.1 years) who played in an U-19 school soccer competition (composed of one group with four teams and another group with three teams, followed by semifinals and final) were examined before three matches, which lasted 70 min. Seventeen athletes had a 24-h rest interval between each match (GGG group), while 18 athletes had a 48-h rest interval between the second and third matches (GG48hG group). Total Quality Recovery, countermovement jump, 10-m sprint, and maximum lumbar isometric strength were measured. The internal load of each match was calculated by the product of the session Rating of Perceived Exertion and match time. There was a 22% reduction in Total Quality Recovery (p< 0.001) and 12% in 10-m sprint performance (p< 0.001) before the third match in the GGG group, while the GG48hG group showed no changes for the same variables (p> 0.05). The countermovement jump decreased before the second match in both groups (GGG= 12% and GG48hG= 10%; p< 0.001), with no difference between groups (p> 0.05). In addition, both groups showed no changes in the isometric strength or the internal load match over the games (p> 0.05). Despite not providing complete muscle recovery, a 48-h interval between the second and third matches seems to have minimized the reduction of muscle performance due to consecutive matches.

Resumo O contexto brasileiro de desigualdades sociais e barreiras no acesso aos serviços de saúde pode agravar a situação da pandemia de COVID-19, que já afeta todos os estados da federação, com a curva crescente de aumento de casos confirmados e mortes. O governo dos países e os agentes do campo científico têm buscado evidências para as melhores práticas de prevenção e controle da transmissão, e cuidado da infecção e doença, incluindo medidas de diagnóstico, tratamento e de atenção à saúde. A estratégia de testagem em larga escala, visando o diagnóstico precoce, quarentena dos casos leves identificados, bem como dos contactantes, e cuidado adequado dos casos graves, tem sido revisada e indicada como uma das medidas eficientes para o controle da pandemia em vários países do mundo. O artigo tem como objetivo discutir os desafios da testagem e do diagnóstico de COVID-19 no Brasil.

Abstract The Brazilian context of social inequalities and barriers in accessing health services may deteriorate the situation of the COVID-19 pandemic, which already affects all Brazilian federative states, with the growing curve of increasing confirmed cases and deaths. National governments and scientific field agents have been looking for evidence for the best practices of prevention and control of transmission, and care of infection and disease, including diagnosis, treatment, and health care measures. The large-scale testing strategy, aimed at early diagnosis, quarantine of the mild cases identified, as well as those of the contacts, and adequate care of severe cases, has been revised and indicated as one of the efficient pandemic control measures in several countries in the world. This paper aims to discuss the challenges of COVID-19 testing and diagnosis in Brazil.

ABSTRACT The objective of this work was to evaluate the effect of extremely low-oxygen storage on the aerobic, anaerobic metabolism and overall quality maintenance of ‘Royal Gala’ and ‘Galaxy’ apples stored at two temperatures. For ‘Royal Gala’ apples, four treatments were evaluated in a two-factor scheme (2 × 2), with two storage conditions: [1] 1.2 kPa O2 + 2 kPa CO2; [2] 0.8 kPa O2 + 1.6 kPa CO2; and two temperatures (1.0 and 1.5 °C). For ‘Galaxy’ apples, the experiment was conducted in a two-factor (3 × 2) scheme with three storage conditions: [1] 1.2 kPa O2 + 2 kPa CO2; [2] 0.8 kPa O2 + 1.6 kPa CO2; [3] 0.4 kPa O2 + 1.2 kPa CO2, and two temperatures (1.0 and 1.5 °C). The ‘Royal Gala’ apples stored under 0.8 kPa O2 + 1.6 kPa CO2 at 1.5 °C had higher flesh firmness, healthy fruit and soluble solids along with lower incidence of flesh breakdown and mealiness. The ‘Galaxy’ apples stored under 0.4 kPa O2 + 1.2 kPa CO2, at temperature of 1.5 °C had lower 1-carboxylic acid-1-aminocyclopropane (ACC) oxidase enzyme activity, ethylene production, occurrence of mealiness, when compared to 1.0 °C. The storage of these two cultivars at 1.5 °C with extremely low O2 partial pressures is possible. The possibility to increase the storage temperature from 1 °C, which is traditionally used for these mutants, to 1.5 °C allows to keep better quality with the advantage of energy saving.

RESUMO O uso comercial de híbridos de cebola é preferido pelos produtores. Em contrapartida, a produção de sementes híbridas de cebola é extremamente ineficiente. Isso se deve ao fato do uso de linhagens obtidas por sucessivas autofecundações assumindo o efeito de depressão por endogamia per se. Diante disso, faz-se necessário buscar novas alternativas capazes de reduzir o efeito de depressão por endogamia nas linhagens. Objetivou-se neste trabalho avaliar possíveis diferenças de polimorfismo e níveis de heterozigosidade de linhagens obtidas de plantas intercruzadas e autofecundadas. Foram utilizadas 12 populações de cebola pertencentes ao programa de melhoramento da Bayer (Granex, IPA-1, IPA-2 e IPA-3), obtidas por autofecundação de uma planta, entrecruzamento de duas plantas ou intercruzamento de três plantas. Três indivíduos de cada linhagem foram utilizados nas análises. As amplificações foram realizadas a partir do uso de 8 microsatélite primers com maior polimorfismo, de acordo com estudos de caracterização de germoplasma realizados pela CITA-Espanhola. A heterozigosidade geralmente diminui com a autofertilização e aumenta à medida que mais plantas são usadas no cruzamento. Os marcadores SSRs usados no presente estudo foram eficientes para detectar variabilidade nos diferentes backgrounds genéticos. Com os resultados obtidos, sugere-se realizar a obtenção de híbridos entre as diferentes combinações e analisar o desempenho per se das diferentes modalidades de obtenção de linhagens propostas na presente pesquisa.

ABSTRACT The commercial use of onion hybrids is preferred by producers. In contrast, the production of hybrid onion seeds is extremely inefficient. This is due to the use of lineages obtained by successive self-fertilizations assuming the effect of inbreeding depression per se. Therefore, it is necessary to understand new alternatives to reduce the effect of inbreeding depression in the strains. The objective of this study was to evaluate possible differences in polymorphism and levels of heterozygosity of strains obtained from intercrossed and self-fertilized plants. Twelve onion populations belonging to Bayer’s breeding program (Granex, IPA-1, IPA-2 and IPA-3) were used, obtained by self-fertilization of one plant, intercrossing of two plants or intercrossing of three plants. Three individuals from each strain were used in the analyzes. The amplifications were performed using 8 microsatellite primers with greater polymorphism, according to germplasm characterization studies carried out by CITA. Heterozygosity generally decreases with self-fertilization and increases as more plants are used in the cross. The SSR markers used in the present study were efficient in detecting variability in different genetic backgrounds. With the results obtained, it is suggested to carry out the obtaining of hybrids between the different combinations and to analyze the performance per se of the different modalities of obtaining strains proposed in the present research.

ABSTRACT The Gala cultivars represent about 65% of the Brazilian apple production, however, it has a short harvest period, making necessary to use technologies that anticipate or delay fruit maturation. A widely used technology is the preharvest application of growth regulators. The objective of this investigation was to evaluate the effect of preharvest application of naphthalene acetic acid (NAA) combined with aminoethoxyvinylglycine (AVG) and 2-chloroethylphosphonic acid (Ethephon), on the quality of ‘Brookfield’ apple at harvest and after eight months controlledatmosphere (CA)(1.2 kPa O2 and 2.0 kPa CO2) storage followed by seven days of shelf life at 20 °C. The treatments were: [1] Control: application of water only; [2] NAA (40 g.ha-1); [3] NAA (40 g·ha-1) plus Ethephon (2.0 L·ha-1 24% of active ingredient), and [4] NAA (40 g·ha-1) plus AVG (0.83 kg.ha-1 15% of active ingredient). At harvest, fruit treated with NAA presented a higher level of starch degradation, ethylene production and respiration rate. Fruit treated with NAA plus AVG maintained better quality after eight months under CA storage plus seven days of shelf life, due to higher healthy fruit amount and higher flesh firmness, but this combination reduced the red skin color index. Additionally, NAA plus Ethephon may be an alternative to maintain the quality of ‘Brookfield’ apple during storage in comparison to the application of NAA isolated.