Abstract Health professionals working to mitigate the COVID-19 pandemic are one of the main risk groups for the disease, being prioritized for vaccination. Considering this, the aim of this study was to analyze the immune response of these professionals immunized with CoronaVac in the first and second doses. Blood samples were collected after the first and second doses of the vaccine (CoronaVac) and used to investigate hematological and biochemical parameters, analysis of immunoglobulin production, cytokines, and gene expression profile, as well as the identification of subsets of immune cells. Post-first dose immunological phenotypic memory (CD27+) profiles (T CD4+, TCD8+ and CD19+) showed a significant increase, as did Monocyte APCs (CD80+HLA-DR+) in relation to the second dose. The cytokines IL-2, IL-6 and IFN-° showed increased values in relation to the other analyzed cytokines. The Th2/Th17 profile in the second dose was characterized by gene expression analysis. The production of IgM and IgG after vaccination showed statistically significant values in the comparison between doses. CoronaVac showed activation of APCs monocytes, memory response of T and B lymphocytes, with immunoglobulins production. This set of responses is characterized by the Th2/Th17 immunological profile.

SUMMARY Acromegaly caused by ectopic growth hormone-releasing hormone (GHRH)-secreting tumor is exceedingly rare. We report a case of acromegaly secondary to GHRH secretion by an incidentally diagnosed pulmonary neuroendocrine tumor (NET) and review 47 similar cases in literature. A 22-year-old male patient presented with symptoms of pituitary apoplexy. Magnetic resonance imaging (MRI) showed apoplexy of a pituitary adenoma. Routinely prior to surgery, a chest radiography was performed which revealed a mass in the left lung. During investigation, the patient was diagnosed with metastatic GHRH-secreting pulmonary NET. In retrospect, it was noted that the patient had pituitary hyperplasia 20 months prior to the MRI which showed the presence of a pituitary adenoma. The histological findings confirmed somatotroph hyperplasia adjacent to somatotropinoma. This case suggests that GHRH secretion can be associated with pituitary hyperplasia, which may be followed by pituitary adenoma formation.

The synthesis, characterization, cytotoxicity and antitumor activity of platinum(II) complexes coordinated to O-glycoside and analogous is reported. Through the acute toxicity tests, it was possible to fit the complexes into categories according to the OECD protocol. The cis-[PtCl2(C6H14S2O2)-κ2S] complex was classified in category 3 and the cis-[PtCl2(C26H38O12S2)-κ2S] complex was classified in category 4. This result demonstrates the lower toxicity achieved by the glycoside in comparison with its analogous system. Evaluations of tumor masses extracted from mice inoculated with Ehrlich carcinoma demonstrated percent inhibition similar to cisplatin and synthesized platinum complexes. Cisplatin showed 59% inhibition, while cis-[PtCl2(C6H14S2O2)-κ2S] and cis-[PtCl2(C26H38O12S2)-κ2S] complexes presented 57% and 59%, respectively. Although the glycosidic compounds exhibit tumor activity, reported in the literature, the presence of platinum ion was shown to be determinant for antitumor action according with the compounds tested, since the glycosidic ligand presented tumor inhibition of only 38%, with a similar antitumor activity in relation to cisplatin.

The use of bioprocess to convert low valued biomass or agroindustrial byproducts into high-value chemicals is an emerging area. However, laboratories usually use outdated analytical techniques to identify bioproducts, losing valuable information, such as the identification of unexpected compounds. In this work, we presented a successful systematic and modern analytical approach based on direct infusion mass spectrometry (DIMS) for an automated high-throughput screening of untargeted compounds from glycerin bioconversion process. We describe advantages of DIMS and its combined application with chemometrics towards an untargeted metabolomics approach to analyze several samples in a short time (11 samples per h). Three batches with 34 samples from the bioconversion of glycerin using several filamentous fungi strains were analyzed. Batch 347 was selected as promising, since high value chemicals, such as amide, phenolic and acid compounds, were identified. The platform presented was fast, robust, and versatile, then it could be applied to different bioprocesses.

Abstract In this study, we developed and validated a chromatographic method by High-performance Liquid Chromatography coupled to Diode-Array Ultraviolet Detector for quantification of acetaminophen in small volumes of plasma. This analytical method is particularly suitable for studies of gastric emptying time and pharmacokinetics in small rodents. Orally administered acetaminophen is promptly and completely absorbed in the intestines, with negligible absorption from the stomach. Owing to these kinetic features, acetaminophen can be used to study gastric emptying. The newly-validated analytical method was employed to investigate whether plants used as anti-dyspeptics in the Brazilian traditional medicine affect the gastric emptying. Lyophilized aqueous extracts from leaves of Baccharis trimera (Less) DC., Asteraceae (carqueja), Maytenus ilicifolia Mart. Ex Reissek, Celastraceae (espinheira-santa) and Lippia alba (Mill.) N.E.Br. ex Britton & P. Wilson, Verbenaceae (erva-cidreira) were administered by gavage to female Wistar rats 30 min before a single oral dose of acetaminophen (50 mg/kg). L. alba extract (50 mg/kg) did not alter acetaminophen plasma concentration versus time curve an indication that it has no effect on gastric emptying. Extracts of B. trimera (50 mg/kg) and M. ilicifolia (30 and 50 mg/kg, but not 10 mg/kg), however, slightly delayed gastric emptying. M. ilicifolia given by intraperitoneal route (30 mg/kg) also retarded gastric emptying. In conclusion, the newly-validated analytical method is adequate for studying the effects of medicinal plants on gastric emptying.

Resumo Introdução As diretrizes sobre rinite alergica visam atualizar os conhecimentos sobre a doença e os cuidados para com esses pacientes. A iniciativa designada "Rinite Alergica e seu Impacto na Asma", cujo relatorio inicial foi publicado em 2001 e atualizada em 2008 e 2010, tem sido muito bem sucedida na disseminaçao de informaçoes e evidencias, bem como na formulaçao da classificaçao de gravidade e proposta de sistematizaçao do tratamento. Entretanto, visando a participaçao de outros profissionais medicos no atendimento da rinite alergica, e importante o desenvolvimento de algoritmos que indiquem com precisao o que deve e pode ser feito regionalmente. Objetivo Atualizar o III Consenso Brasileiro sobre Rinites-2012, com elaboraçao de algoritmo para conduta da rinite alergica. Método Foram convidados 24 especialistas indicados pelas Associaçao Brasileira de Alergia e Imunologia, Associaçao Brasileira de Otorrinolaringologia e Cirurgia Cervico-Facial e Sociedade Brasileira de Pediatria para atualizaçao do documento de 2012. Resultados A atualizaçao do ultimo Consenso Brasileiro sobre Rinites, incorporou e adaptou para a realidade brasileira as informaçoes relevantes publicadas em todos os documentos da Iniciativa "Rinite Alergica e seu Impacto na Asma", trazendo novos conceitos como a rinite alergica local, novos medicamentos e metodos de avaliaçao de tratamento. Conclusão Proposto um fluxograma de tratamento para a rinite alergica.

Abstract Introduction The guidelines on allergic rhinitis aim to update knowledge about the disease and care for affected patients. The initiative called "Allergic Rhinitis and its Impact on Asthma", initially published in 2001 and updated in 2008 and 2010, has been very successful in disseminating information and evidence, as well as providing a classification of severity and proposing a systemized treatment protocol. In order to include the participation of other medical professionals in the treatment of allergic rhinitis, it is important to develop algorithms that accurately indicate what should and can be done regionally. Objective To update the III Brazilian Consensus on Rhinitis - 2012, with the creation of an algorithm for allergic rhinitis management. Methods We invited 24 experts nominated by the Brazilian Association of Allergy and Immunology, Brazilian Association of Otorhinolaryngology and Head and Neck Surgery and Brazilian Society of Pediatrics to update the 2012 document. Results The update of the last Brazilian Consensus on Rhinitis incorporated and adapted the relevant information published in all "Allergic Rhinitis and its Impact on Asthma" Initiative documents to the Brazilian scenario, bringing new concepts such as local allergic rhinitis, new drugs and treatment evaluation methods. Conclusion A flowchart for allergic rhinitis treatment has been proposed.

Abstract Introduction Vanilla (Vanilla planifolia Jacks ex Andrews), is an orchid, whose fruit has outstanding organoleptic characteristics, being the second most expensive spice after saffron and used as flavoring. Due to changes in the climate pattern in the Totonacapan producing region, in Veracruz, Mexico, the productivity of vanilla has decreased, but in other regions such as the Huasteca Potosina, commercial production represents an option for local producers. Therefore, in this study the quality of the vanilla from 14 sites of eleven localities, belonging to eight municipalities of the Huasteca Potosina, San Luis Potosí, Mexico, was evaluated. Method Vanilla fruits were harvested from each locality, in physiological maturity at 32 weeks of age (224 days after pollination), and they were subjected to the same process of curing and variables such as size, humidity percentage water activity, total soluble sugars and aroma compounds in the cured fruit were evaluated. Results It was found that the predominant sugar in vanilla fruits was glucose, with 1.55-4.28%. The fruits from 60 % of the collect sites achieve the minimum standard length. Vanillic acid was the component of the aroma with the greatest variation. The vanillin content of the fruits from all the municipalities had more than 1.2 %, and only the fruit samples from two sites (Tixcuayuca3 and Tamala) achieve the minimum of 2.0 % required by NOM-182-SCFI-2011. Discussion and conclusion There is a significant variation in the content of aromatic compounds, with a low ratio between the aroma compounds (p-hydroxybenzoic, vanillic acid and p-hydroxybenzaldehyde) and vanillin from 6.97-11.87 of the vanilla cured fruits from 14 sites of the Huasteca Potosina, Mexico. These differences are considered the result of the interaction of the environment with the species, culture conditions, nutrition, among others. Despite this it was found several fruits which stand out for their organoleptic quality, mainly for the vanillin content, such the ones grown at the municipalities of Matlapa, Xilita and Tamazunchale.

Resumen Introducción La vainilla (Vanilla planifolia Jacks. ex Andrews), es una orquídea cuyo fruto beneficiado presenta características organolépticas sobresalientes, siendo la segunda especia más costosa después del azafrán y utilizada como saborizante y aromatizante. Debido a cambios en el patrón climático en la región productora del Totonacapan, en Veracruz, México, la productividad de la vainilla ha disminuido, pero en otras regiones como la Huasteca Potosina, la producción comercial representa una opción para los productores locales. Por lo anterior en este estudio se evaluó la calidad de la vainilla procedente de 14 sitios de once localidades, pertenecientes a ocho municipios de la Huasteca Potosina, San Luis Potosí, México. Método Se cosecharon frutos de vainilla de cada localidad, en madurez fisiológica de 32 semanas de edad (224 días después de polinización), y se sometieron a un mismo proceso de beneficio y posteriormente se evaluaron variables como dimensión, porcentaje de humedad, actividad de agua, contenido de azúcares solubles totales y compuestos del aroma en el fruto beneficiado. Resultados Se encontró que el azúcar predominante en frutos de vainilla beneficiado fue la glucosa, con 1.55-4.28 %. Los frutos provenientes del 60% de los lugares de colecta cumplieron con la norma de longitud mínima. El ácido vaníllico fue el componente del aroma con mayor variación. El contenido de vainillina de los frutos provenientes de todos los municipios tuvieron más de 1.2 %, y solo las muestras de fruto de dos sitios (Tixcuayuca3 y Tamala) cumplieron con el mínimo de 2.0 % que exige la NOM-182-SCFI-2011. Discusión y conclusión Existe una variación significativa en el contenido de compuestos aromáticos, con una baja relación entre los compuestos del aroma (p-hidroxibenzóico, ácido vaníllico y p-hidroxibenzaldehído) y vainillina de 6.97-11.87 de los frutos de vainilla beneficiadas procedentes de 14 sitios de la Huasteca Potosina, México. Estas diferencias se consideran el resultado de la interacción del medio con la especie, condiciones del cultivo, nutrición, entre otras. A pesar de esto se pueden encontrar frutos que sobresalen por su calidad organoléptica, principalmente por el contenido de vainillina, como los frutos procedentes de los municipios de Matlapa, Xilita y Tamazunchale.

RESUMO O procedimento diagnóstico neuromuscular é complexo. O conhecimento da frequência relativa das doenças neuromusculares em uma população é importante para utilização dos testes diagnósticos mais apropriados. Objetivo: Relatar a frequência relativa de doenças neuromusculares em um centro de referência. Métodos: Revisão de prontuários de pacientes com suspeita de miopatia em 17 anos. Resultados: Dentre 3412 exames, 1603 (46,98%) foram confirmatórios: 782 (48,78%) estudos moleculares e 821 (51,21%) biópsias musculares. Os diagnósticos mais frequentes foram: distrofinopatia 460 (28,70%), mitocondriopatia 330 (20.59%), atrofia muscular espinhal 158 (9,86%), distrofia muscular cintura-membros 157 (9,79%), distrofia miotônica de Steinert 138 (8,61%), distrofia muscular face-escápulo-umeral 99 (6,17%) e outros diagnósticos 261 (16,28%). Conclusão: Utilizando as técnicas diagnósticas atualmente disponíveis em nosso serviço, o diagnóstico específico do subtipo de distrofia muscular cintura-membros foi obtido em 61% dos pacientes. O diagnóstico neuromuscular apropriado é importante para o aconselhamento genético, orientações de reabilitação e tratamento precoce de complicações respiratórias e cardíacas.

ABSTRACT The diagnostic procedure in neuromuscular patients is complex. Knowledge of the relative frequency of neuromuscular diseases within the investigated population is important to allow the neurologist to perform the most appropriate diagnostic tests. Objective: To report the relative frequency of common neuromuscular diagnoses in a reference center. Methods: A 17-year chart review of patients with suspicion of myopathy. Results: Among 3,412 examinations, 1,603 (46.98%) yielded confirmatory results: 782 (48.78%) underwent molecular studies, and 821 (51.21%) had muscle biopsies. The most frequent diagnoses were: dystrophinopathy 460 (28.70%), mitochondriopathy 330 (20.59%), spinal muscular atrophy 158 (9.86%), limb girdle muscular dystrophy 157 (9.79%), Steinert myotonic dystrophy 138 (8.61%), facioscapulohumeral muscular dystrophy 99 (6.17%), and other diagnoses 261 (16.28%). Conclusion: Using the presently-available diagnostic techniques in this service, a specific limb girdle muscular dystrophy subtype diagnosis was reached in 61% of the patients. A neuromuscular-appropriate diagnosis is important for genetic counseling, rehabilitation orientation, and early treatment of respiratory and cardiac complications.

Resumen: El presente estudio tiene como objetivo estudiar la estructura factorial, fiabilidad y validez del Contínuum de Salud Mental (MHC-SF) de Keyes (2009) en el contexto ecuatoriano. La escala fue aplicada a dos muestras, una de 100 personas que funcionó como piloto y otra de 550 personas residentes en la ciudad de Cuenca- Ecuador, a quienes también se aplicó una adaptación del Perfil PERMA de Butler y Kern (2016) para comprobar la validez convergente y discriminante. Se observó que la aplicación en esta población presenta adecuados niveles de ajuste y alta consistencia interna (α= .91) y un ajuste general adecuado de la estructura de tres factores: bienestar psicológico, bienestar social, bienestar emocional propuestos por el autor, dando valores de RMSEA= .045, CFI= .996, χ2(52)= 109.048 (p= .000), GFI= .997 y TLI= 0.993. El modelo bifactorial presenta valores de RMSEA= .042, CFI= .996, χ2 (52)= 103.040 (p= .000), GFI= .997, TLI= 0.993. Se concluye que la versión en español de MHC-SF (Keyes, 2009) y la versión bifactorial son herramientas apropiadas para medir la salud mental en el contexto ecuatoriano

Abstract: Health Continuum (MHC-SF) of Keyes (2009) in the Ecuadorian context. The scale was applied to two samples, one of 100 people that worked as a pilot and another of 550 people resident in the city of Cuenca-Ecuador, who also were applied an adaptation of the PERMA Profile of Butler and Kern (2016) to verify the convergent and discriminant validity. It was observed that the application in this population presents adequate levels of adjustment and high internal consistency (α=.91) and an adequate general adjustment of the structure of three factors: psychological well-being, social welfare and emotional well-being proposed by the author whose main fit values are RMSEA= .045, CFI= .996, χ2(52)= 109.048 (p= .000), GFI= .997 y TLI= 0.993. The bifactor model presents good values as well: RMSEA= .042, CFI= .996, χ2(52) = 103.040 (p= .000), GFI= .997, TLI= 0.993. We conclude that the Spanish version of MHC-SF (Keyes, 2009) and the bifactor model are appropriate tools to measure mental health in the Ecuadorian context

OBJETIVO: Analisar questões não redundantes sobre independência nas atividades da vida diária de idosos que representem o espectro de dependência em idosos. MÉTODOS: Projeto multicêntrico com amostra populacional probabilística de 5.371 idosos residentes em São Paulo, SP, Rio de Janeiro, RJ, Bambuí, MG, e Fortaleza, CE, em 2008. Foi realizado inquérito domiciliar e aplicado questionário com 20 atividades da vida diária dos idosos para autoavaliação da dificuldade/necessidade de ajuda para realizá-las. As respostas foram analisadas segundo: a prevalência de alguma dificuldade ou necessidade de ajuda para cada atividade da vida diária; a frequência de não resposta; e o agrupamento das atividades numa análise fatorial. RESULTADOS: As atividades pessoais (e.g., vestir-se) tiveram prevalência de dificuldade ou necessidade de ajuda referida baixa quando comparadas às atividades instrumentais (e.g., fazer compras), além de terem taxas de respostas inválidas mais baixas. Foram identificados três fatores de agrupamento das atividades da vida diária: mobilidade (andar 100 m); necessidades pessoais (tomar banho); e o que outra pessoa pode fazer pelo idoso (lavar roupa). As atividades da vida diária com maiores autovalores em cada grupo foram analisadas à luz da prevalência de necessidade de ajuda referida e da proporção de respostas válidas. Três atividades foram selecionadas como representativas do espectro de dependência e bem compreendidas pelos idosos: levantar da cama, banhar-se e andar 100 m. CONCLUSÕES: Com três atividades da vida diária podemos ter um instrumento de rastreio simples e confiável capaz de identificar idosos com necessidade de ajuda no dia a dia. A estimativa de demanda por cuidados na vida diária é um indicador importante para o planejamento e gestão dos serviços de saúde dentro do paradigma das doenças crônicas e do envelhecimento populacional.

OBJECTIVE: To analyze non-redundant questions on independence in activities of daily living in the elderly, representing the spectrum of dependency. METHODS: Multicenter project with a probabilistic population sample of 5,371 elderly residents in Sao Paulo, SP, Rio de Janeiro, RJ, Fortaleza, CE and Bambui, MG in 2008. A household survey was carried out and a questionnaire with 20 activities of daily living applied for the elderly to self-assess the difficulty/need for help in performing them. The responses were analyzed according to: the prevalence of some kind of difficulty/need for help for each activities of daily living, the frequency of non-response, and the grouping of activities in factor analysis. RESULTS: The personal activities (e.g., dressing) have, on average, a low prevalence of difficulty or need for help, compared to instrumental activities (e.g., shopping), and have lower rates of non- response. In factor analysis it was possible to identify three factors grouping the activities of daily living: one relative to mobility (e.g., walking 100 m), another for personal needs (e.g., bathing) and one relative to what someone else can do for the elderly (e.g., washing clothes). The activities of daily living with the highest eigenvalues in each group were also analyzed in the light of the prevalence of reported need for help and the proportion of non response. Three activities of daily living were selected as representing the spectrum of dependency and being well understood by the elderly - getting out of bed, bathing and walking 100 m. CONCLUSIONS: With only three activities of daily living we can have a simple and reliable screening instrument capable of identifying elderly in need of help in daily life. Estimating demand for care on a daily basis is an important indicator for planning and administration of health services within the paradigm of chronic diseases and population aging.

OBJETIVO: Analizar aspectos no redundantes sobre independencia en las actividades de la vida diaria de ancianos que representen el espectro de dependencia en ancianos. MÉTODOS: Proyecto multicéntrico con muestra poblacional probabilística de 5.371 ancianos residentes en Sao Paulo, SP, Rio de Janeiro, RJ, Bambuí, MG y Fortaleza, CE, (Brasil), en 2008. Se realizó pesquisa domiciliar y se aplicó cuestionario con 20 actividades de la vida diaria de los ancianos para auto-evaluación de la dificultad/necesidad de ayuda para realizarlas. Las respuestas fueron analizadas de acuerdo a: la prevalencia de alguna dificultad o necesidad de ayuda en cada actividad de la vida diaria; la frecuencia de respuesta no válidas; y el agrupamiento de las actividades en un análisis factorial. RESULTADOS: Las actividades personales (ej: vestirse) tuvieron prevalencia de dificultad o necesidad de ayuda referida baja al compararse con las actividades instrumentales (ej: hacer compras), además de tener tasas de respuestas: inválidas más bajas. Se identificaron tres factores de agrupamiento de las actividades de la vida diaria: movilidad (andar 100m); necesidades personales (bañarse) y lo que otra persona puede hacer por el anciano (lavar ropa). Las actividades de la vida diaria con mayores auto-valores en cada grupo fueron analizadas a la luz de la prevalencia de necesidad de ayuda referida y de la proporción de respuestas válidas. Tres actividades fueron seleccionadas como representativas del espectro de dependencia y bien comprendidas por los ancianos: levantar de la cama, bañarse y andar 100 m. CONCLUSIONES: Con tres actividades de la vida diaria podemos tener un instrumento de rastreo simple y confiable capaz de identificar ancianos con necesidad de ayuda en el día a día. La estimativa de demanda por cuidados en la vida diaria es un indicador importante para la planificación y gestión de los servicios de salud dentro del paradigma de las enfermedades crónicas y del envejecimiento poblacional.

OBJECTIVE: Previous studies have demonstrated a relationship between brain oxidative stress and cardiovascular regulation. We evaluated the effects of central catalase inhibition on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke. METHODS: Male Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SH) (16 weeks old) were implanted with a stainless steel guide cannula leading into the fourth cerebral ventricle (4th V). The femoral artery and vein were cannulated for arterial pressure and heart rate measurement and drug infusion, respectively. The rats were exposed to sidestream cigarette smoke for 180 minutes/day, 5 days/week for 3 weeks (CO: 100-300 ppm). The baroreflex was tested using a pressor dose of phenylephrine (8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (50 μg/kg, bolus). Cardiovascular responses were evaluated before and 5, 15, 30 and 60 minutes after injection of a catalase inhibitor (3-amino-1,2,4-triazole, 0.001 g/100 μL) into the 4th V. RESULTS: Vehicle administration into the 4th V did not affect the cardiovascular response, whereas administration of the central catalase inhibitor increased the basal HR and attenuated the bradycardic peak (p<0.05) to a greater extent in WKY rats exposed to sidestream cigarette smoke than in WKY rats exposed to fresh air. However, in spontaneously hypertensive rats, the effect of the catalase inhibitor treatment was stronger in the fresh air condition (p<0.05). CONCLUSION: Administration of a catalase inhibitor into the 4th V combined with exposure to sidestream cigarette smoke has a stronger effect in WKY rats than in SH rats.

Chrysopogon zizanioides (L.) Roberty, Poaceae, is a plant widely used in northeast Brazil in folk medicine for the treatment of various pathological conditions, including inflammatory pain. The present study evaluated the antinociceptive and anti-inflammatory effects of C. zizanioides essential oil (EO) in rodents. EO was further characterized by GC/MS. The major components of EO were identified as khusimol (19.57%), E-isovalencenol (13.24%), α-vetivone (5.25%), β-vetivone (4.87%) and hydroxy-valencene (4.64%). Following intraperitoneal injection (i.p.), EO at 50 and 100 mg/kg significantly reduced the number of writhes (51.9 and 64.9%, respectively) and the number of paw licks during phase 2 (56.7 and 86.2%, respectively) of a formalin model when compared to control group animals. However, EO-treated mice were ineffective at all doses in hot-plate and rota-rod tests. The EO inhibited the carrageenan-induced leukocyte migration to the peritoneal cavity in a dose-dependent manner (34.7, 35.4, and 62.5% at doses of 25, 50 and 100 mg/kg, respectively). In the paw edema test, the EO (100 mg/kg) inhibited all three phases of the edema equally well, suggesting that the EO has a non-selective inhibitory effect on the release or actions of these mediators. Our results suggest possible antinociceptive and anti-inflammatory effects of the EO.

The present work investigated the antinociceptive and antiinflammatory activities of the Porophyllum ruderale (Jacq.) Cass., Asteraceae, aqueous extract (PRAE). For this purpose, acetic acid writhing, paw licking induced by formalin, hot-plate and pleurisy tests were performed. The doses of 100, 200 and 400 mg/kg (p.o.) significantly inhibited the writhing 63.4, 89.6 and 94.8%, respectively, in comparison with control group. The lick of the paw 1st phase was reduced at the dose of 400 mg/ kg (24.9%), while the 2nd phase had reduction at doses 200 and 400 mg/ kg (23.1 and 34.4%), respectively. The PRAE inhibited the carrageenaninduced neutrophil migration to the peritoneal cavity in a higher dose (p<0.05). Taken together, our results suggest that the PRAE can constitute target potential for use in therapies of the pain and inflammation.

O trabalho focaliza a escola como lócus de formação. Analisa o contexto de uma escola de sucesso da rede municipal de Campo Grande, Estado do Mato Grosso do Sul, descrevendo-o em suas características. Entre estas, destaca a gestão e o clima escolar. Para tanto, entrevista professores e gestores sobre a organização da escola, o estilo de gestão, bem como sobre os seus efeitos no trabalho do professor e no desempenho dos alunos. O exame das entrevistas permitiu construir algumas categorias que definem o ethos da instituição: o porte pequeno, o trabalho em equipe, as relações democráticas e o compromisso com os alunos. A análise destaca o profissionalismo da gestão e da sua visão prospectiva sobre a tarefa educativa, estimulando os professores a enfrentarem os desafios e compartilhando com eles as formas de superar as dificuldades. O estudo aponta a importância da rede de relações que se estabelece no contexto da escola como forma de socialização da profissão, construção da identidade docente e espaço de formação de professores a partir da prática.

This work focuses on the school as a place for teachers education. It analyses the context of a successful school in the Campo Grande municipal system, in the State of Mato Grosso do Sul. The article describes its characteristics, with emphasis on the school management and the school climate. Teachers and managers were interviewed about the school, its organization and management as well as about the way these dimensions affect their work and the students’ performance. By examining the interviews, it was possible to build up some of the categories that define the ethos of the school: the fact of it being small, team-work, democratic relationships and commitment to the students. The analysis highlights the professionalism of the management and its prospective vision of the educational task, which encourages the teachers to face up to the challenges and shares with them ways of overcoming the difficulties. It points out the importance of the network of relationships that are established within the context of the school as a way of socializing the profession, constructing a teaching identity and establishing a place were teachers can learn starting with teaching practice.