Resumo A doença coronariana (DCC) tem sido associada a significativa morbidade e mortalidade em todo o mundo. Embora ainda sejam controversos, vários estudos têm demonstrado a associação de infecções por M. pneumoniae com aterosclerose. Avaliamos a possível associação de infecções por micoplasma em pacientes com diagnóstico de aterosclerose pelos métodos ELISA e PCR. Amostras de tecido aterosclerótico e amostras de sangue foram coletadas para a detecção de anticorpos contra micoplasma (IgA) por ELISA de 97 pacientes com doença arterial coronariana (DAC). IgA, IgG e IgM específicos para M. pneumoniae foram medidos usando o Anti-M. pneumoniae IgA / IgG / IgM ELISA. A detecção de M. pneumoniae visando o gene de adesão P1 foi realizada por PCR. A infecção aguda por M. pneumoniae foi diagnosticada em 43,3% (42) dos pacientes pela PCR. Os anticorpos específicos para M. pneumoniae foram detectados em 36,1% (35) dos pacientes. Vinte e cinco (25,8%) casos tinham anticorpos IgG, 15 (15,5%) casos tinham anticorpos IgM, 3 (3,1%) casos tinham anticorpos IgA, 10 (10,3%) casos tinham anticorpos IgM + IgG e 1 (1%) caso de cada um tinha anticorpos IgM + IgA e IgG + IgA. Nenhum dos casos foi positivo para os três anticorpos. A análise do coeficiente de correlação de Pearson revelou uma excelente correlação entre o PCR e os resultados sorológicos (r = 0,921, p < 0,001). A maioria (17, 40,5%) dos pacientes positivos para M. pneumoniae está na faixa etária de 41-50 anos, seguida por 10 (23,8%) pacientes na faixa etária de 61-70 anos e 2 (4,8%) pacientes tinham > 70 anos de idade. Nosso estudo relatou uma prevalência incomumente maior de M. pneumoniae por testes sorológicos (36,1%) e PCR (43,3%). Embora a hipótese da associação de M. pneumoniae e DAC ainda não tenha sido comprovada, a prevalência incomumente alta de M. pneumoniae em pacientes com DAC indica uma associação, se não, no desenvolvimento de aterosclerose.

Abstract Coronary heart disease (CHD) has been associated with significant morbidity and mortality worldwide. Although remain controversial, several studies have demonstrated the association of M. pneumoniae infections with atherosclerosis. We evaluated the possible association of mycoplasma infections in patients diagnosed with atherosclerosis by ELISA and PCR methods. Atherosclerotic tissue samples and blood samples were collected for the detection of mycoplasma antibodies (IgA) by ELISA from the 97 patients with coronary artery disease (CAD). M. pneumoniae specific IgA, IgG and IgM were measured by using the Anti-M. pneumoniae IgA/IgG/IgM ELISA. Detection of M. pneumoniae targeting the P1 adhesion gene was performed by PCR Acute infection of M. pneumoniae was diagnosed in 43.3% (42) of patients by PCR. The M. pneumoniae specific antibodies were detected in 36.1% (35) of patients. Twenty-five (25.8%) cases had IgG antibodies, 15 (15.5%) cases had IgM antibodies, 3 (3.1%) cases had IgA antibodies, 10 (10.3%) cases had both IgM + IgG antibodies and 1 (1%) case of each had IgM + IgA and IgG + IgA antibodies. None of the cases was positive for all three antibodies. A Pearson correlation coefficient analysis revealed an excellent correlation between the PCR and the serological results (r=0.921, p<0.001). A majority (17, 40.5%) of the M. pneumoniae positive patients are within the 41-50 years of age group, followed by 10 (23.8%) patients in the age group of 61-70 years and 2 (4.8%) patients were >70 years of age. Our study reported an unusually higher prevalence of M. pneumoniae by serological tests (36.1%) and PCR (43.3%). Although the hypothesis of the association of M. pneumoniae and CAD is yet to be proven, the unusually high prevalence of M. pneumoniae in CAD patients indicates an association, if not, in the development of atherosclerosis.

To solve the technical, economic and environmental problems in the remanufacturing process of decommissioned machine tools, this study proposes a module partition method for remanufacturing retired machine tools. Through an analysis of the economic, accuracy, environment-friendly, and functional and physical interactivity principles, this study applies the fuzzy cluster method (FCM) to analyse the modularity of the components to be assembled. It establishes the correlation matrix between the parts, from which a module division scheme is obtained. Finally, a case study is presented to verify the feasibility of the module division method.

Om die tegniese, ekonomiese en omgewingsprobleme in die hervervaardigingsproses van masjiengereedskap wat buite gebruik geraak het, op te los, stel hierdie studie 'n moduleverdelingsmetode voor vir die hervervaardiging van sulke masjiengereedskap. Deur 'n ontleding van die ekonomiese, akkuraatheid, omgewingsvriendelike en funksionele en fisiese interaktiwiteitsbeginsels, pas hierdie studie die "fuzzy cluster" metode (FCM) toe om die modulariteit van die komponente wat saamgestel moet word, te analiseer. Dit bepaal die korrelasiematriks tussen die dele, waaruit 'n module-verdelingskema verkry word. Ten slotte word 'n gevallestudie aangebied om die uitvoerbaarheid van die module-indelingsmetode te verifieer.

ABSTRACT Objective: This study aims to investigate the application of Glisson pedicle transection hepatectomy in the anatomic hepatic resection. Methods: Forty-five patients with hepatocellular carcinoma (HCC) were treated by liver resection using Glisson pedicle transection hepatectomy by the same surgical group during June, 2009 and June, 2012 and their clinical data during the peri-operative period were retrospectively analysed. Follow-up was performed for one-year. Results: In the selected 45 cases, 82.2% (37/45) of the patients underwent anatomical hepatectomy. Bile leakage and bleeding occurred in six cases during the belting of the three main hepatic pedicles, which were treated by local compression or proper stitching. The recurrence rate one-year after surgery was 15.6% (7/45) and the survival rate was 80.0% (36/45) in one-year. Conclusion: Glisson pedicle transection hepatectomy had advantages of embodying regional hepatichilus blocking and avoiding the cumbersome anatomy of hepatichilus vessels. It is also consistent with the principles of radical cure for tumours and precise liver resection.

RESUMEN Objetivo: Este estudio está encaminado a investigar la aplicación de la hepatectomía mediante transección del pedículo de Glisson en la resección hepática anatómica Métodos: Cuarenta y cinco pacientes con carcinoma hepatocelular (HCC) fueron tratados mediante resección hepática usando hepatectomía mediante transección del pedículo glissoniano por el mismo grupo quirúrgico durante junio de 2009 y junio de 2012, y sus datos clínicos durante el período peri-operatorio fueron analizados retrospectivamente. Se realizó un seguimiento de un año. Resultados: De los 45 casos seleccionados, 82.2% (37/45) de los pacientes experimentó hepatectomía anatómica. En seis casos se produjo escape de bilis y sangramiento durante el pinzamiento de los tres pedículos principales. Tanto el escape de bilis como el sangramiento fueron tratados mediante compresión local o sutura adecuada. La tasa de recurrencia un año después de la cirugía fue de 15.6% (7/45) y la tasa de supervivencia fue de 80.0% (36/45) en un año. Conclusión: La hepatectomía mediante transección del pedículo glissoniano tuvo como ventajas incorporar el bloqueo de la región del hilio hepático y evitar la complicada anatomía de los vasos del hilio hepático. También concuerda con los principios de la cura radical de tumores y la resección hepática precisa.