Abstract Total triterpenoids from Antrodia cinnamomea fermentation mycelium (TT-ACFM) were prepared, and their in vitro inhibitory effects on hepatocellular carcinoma were investigated. Human hepatocellular carcinoma HepG2 cells were incubated with TT-ACFM with concentration of 0 (control), 12.5, 25, 50, 100 and 200 μg/mL, respectively. The proliferation of cell was detected using CCK-8 method. The apoptosis of cells was detected by flow cytometry. The migration and invasion of cells was determined using Transwell chamber. The level of reactive oxygen species (ROS) in cells was determined using DCFH-DA method. The expressions of matrix metalloproteinse-2 (MMP-2), matrix metalloproteinse-9 (MMP-9) and vascular endothelial growth factor (VEGF) protein in cells were detected by western blot assays. Results indicate that, TT-ACFM can not only inhibit the proliferation of HepG2 cells and promote their apoptosis, but also inhibit their invasion and invasion. The mechanism may be related to its increase of ROS and down-regulation of MMP-2, MMP-9 and VEGF expression in cells.

Abstract This work aimed to investigate the process parameters of phytosterol esters (PE) synthesis catalyzed by copper oxide-nano hydroxyapatite (CuO-NHAP) and the crystallization purification technology. CuO-NHAP was prepared. The synthesis of PE catalyzed by CuO-NHAP was performed. After Response Surface Methodology optimization, the optimal process parameters were as follows: reaction temperature, 180 oC; reaction time, 6.3 h; acid-alcohol molar ratio, 1.9; catalyst amount, 1.05%. Under these conditions, the esterification rate of phytosterols was 99.8%. The crystallization purification technology of esterification product was explored. The optimal crystallization conditions were as follows: crystallization solvent, octanoic acid; crystallization temperature, 0 oC; solvent-product molar ratio, 4: 1; crystallization time, 10 h. Under these conditions, the unreacted fatty acids and other impurities were affectively removed, and the PE purity in purification product was 98.2%. These synthesis and crystallization purification technologies of PE are relatively green, safe, efficient and economic, and have the potential to be applied to the industrial preparation of PE product.

Abstract In this study, the total triterpenoids of raspberry fruit (TTRF) were prepared, and their effects on human hepatocellular carcinoma SMMC-7721 cells were investigated. SMMC-7721 cells were treated with TTRF with concentration of 0 (control), 50, 100, 200 and 400 μg/mL, respectively. The cell proliferation, cycle and apoptosis were detected. The reactive oxygen species (ROS) in cells and the expression levels of B-cell lymphoma-2 (Bcl-2) and B-cell lymphoma-2 associated X (Bax) in cells were determined. Results showed that, TTRF could obviously inhibit the proliferation of SMMC-7721 cells, arrest most cells in S and G2/M phases, and promote their apoptosis. In addition, TTRF could increase the ROS level, down-regulate the Bcl-2 protein expression and up-regulate the Bax protein expression in cells. In conclusion, cucurbitacin I has inhibitory effect on growth of SMMC-7721cells. The action mechanism may be related to its increasing ROS level and regulating of Bcl-2 and Bax expression in cells.