Abstract Background Early risk stratification with simple biomarkers is essential in patients with non-ST segment-elevation myocardial infarction (NSTEMI). Objective This study aimed to evaluate the association between plasma big endothelin-1 (ET-1) level and the SYNTAX score (SS) in patients with NSTEMI. Methods A total of 766 patients with NSTEMI undergoing coronary angiography were recruited. Patients were divided into three groups: low SS (≤22), intermediate SS (23-32), and high SS (>32). Spearman correlation, smooth curve fitting, logistic regression, and receiver operating characteristic (ROC) curve analysis were performed to evaluate the association between plasma big ET-1 level and the SS. A p-value <0.05 was considered statistically significant. Results There was a significant correlation between the big ET-1 and the SS (r=0.378, p<0.001). The smoothing curve indicated a positive correlation between the plasma big ET-1 level and the SS. The ROC curve analysis showed that the area under the curve was 0.695 (0.661-0.727) and the optimal cutoff of plasma big ET-1 level was 0.35pmol/l. Logistic regression showed that elevated big ET-1 was an independent predictor of intermediate-high SS in patients with NSTEMI, whether entered as a continuous variable [OR (95% CI): 1.110 (1.053-1.170), p<0.001] or as a categorical variable [OR (95% CI): 2.962 (2.073-4.233), p<0.001]. Conclusion In patients with NSTEMI, the plasma big ET-1 level was significantly correlated with the SS. Elevated plasma big ET-1 level was an independent predictor for intermediate-high SS.

Resumo Fundamento A estratificação de risco precoce com biomarcadores simples é essencial em pacientes com infarto do miocárdio sem supradesnivelamento do segmento ST (IAMSSST). Objetivo Este estudo tem o objetivo de avaliar a associação entre nível de big endotelina-1 plasmática (ET-1) e o escore SYNTAX (SS) em pacientes com IAMSSST. Métodos Foram recrutados 766 pacientes com IAMSSST que passaram por angiografia coronária. Os pacientes foram divididos em três grupos: SS baixo (≤22), SS intermediário (23-32), e SS alto (>32). A correlação de Spearman, o ajuste de curva suave, a regressão logística, e a análise de curva característica de operação do receptor (ROC) foram realizados para avaliar a associação entre o nível de big ET-1 plasmática e o SS. Um p-valor <0.05 foi considerado estatisticamente significativo. Resultados Foi identificada uma correlação significativa entre a big ET-1 e o SS (r=0,378, p<0,001). A curva suavizada indicou uma correlação positiva entre o nível de big ET-1 plasmática e o SS. A análise de curva ROC demonstrou que a área sob a curva foi de 0,695 (0,661-0,727) e o ponto de corte ideal do nível de big ET-1 plasmática foi de 0,35 pmol/l. A regressão logística demonstrou que a big ET-1 elevada era um preditor independente de SS intermediário a alto em pacientes com IAMSSST, seja como variável contínua [RC (IC 95%: 1,110 (1,053-1,170), p<0,001] ou como variável categórica [RC (IC 95%: 2,962 (2,073-4,233), p<0,001]. Conclusão Em pacientes com IAMSSST, o nível de big ET-1 plasmática estava significativamente correlacionado ao SS. O nível de big ET-1 plasmática elevado foi um preditor independente para SS intermediário a alto.

ABSTRACT Chilling stress inhibits germination and early seedling growth of Salvia miltiorrhiza Bunge. The present study was carried out to investigate the effects of osmopriming with CaCl2 on the germination and early seedling growth of S. miltiorrhiza under chilling stress (10 ℃). Seeds were soaked in different concentrations of CaCl2 solutions (0, 50, 100, and 150 mM) at 20 ℃ for 24 h in darkness, rinsed, and air-dried. The effects of seed priming were evaluated on germination and survival during subsequent exposure to chilling stress (10 ℃) for 14 d. An unprimed control treatment was sown under optimal temperatures (25 ℃). Results indicated that germination percentage, germination potential, and seedling vigor index of unprimed seeds decreased from 69.2%, 12.4, and 0.092 to 28%, 3.6, and 0.022, respectively, when subjected to chilling stress. Higher malondialdehyde (MDA) content and lower chlorophyll contents (including total chlorophyll, chlorophyll a, and chlorophyll b) were detected in S. miltiorrhiza seedlings. Osmopriming with 100 and 150 mM CaCl2 effectively lessened the negative effects of chilling stress. Germination percentage, germination potential, and seedling vigor index of primed seeds reached 42% and 48%, 6.36 and 8.78, and 0.037 and 0.047, respectively. Meanwhile, CaCl2 priming significantly triggered superoxide dismutase, peroxidase, and catalase antioxidant enzyme activity, enhanced the accumulation of osmoregulation substances (soluble protein, accumulation sugars, and proline), increased the photosynthetic pigment content (total chlorophyll, chlorophyll a, chlorophyll b, and carotenoids), and decreased the MDA content. The most effective concentration of CaCl2 priming was 150 mM.

Abstract: INTRODUCTION: To provide information for cervical cancer screening and vaccination in Henan province, China, the distribution of human papillomavirus (HPV) was analyzed. METHODS: The HPV genotypes were detected using gene array and flow-through hybridization. RESULTS: Overall, 38.1% (1,536/4,033) of the women were human papillomavirus deoxyribonucleic acid (HPV DNA) positive. The prevalence of high-risk HPV types was 32.4%. HPV 16 was the most prevalent genotype (8.9%), followed by HPV 52 (5.8%) and HPV 58 (4.4%). CONCLUSIONS: The data support close surveillance of women for cervical cancer screening, and HPV prophylactic vaccines including HPV16, HPV 52, and HPV 58 might offer greater protection in this area.

Focalização isoeléctrica em camada ultrafina de proteínas de semente foi utilizada para testar a pureza genética de sementes de híbridos abóbora. Na amplitude de pH de 5 a 8, uma banda marcadora macho e duas bandas marcadoras femininas foram encontradas nas linhagens parentais macho e fêmea, respectivamente. Com base nas bandas marcadoras masculina e feminina, a pureza genética de sementes de F1 pode ser facilmente determinada em laboratório.

Ultrathin-layer isoelectric focusing of seed proteins was used to test the genetic purity of pumpkin hybrid seeds. In the pH range of 5-8, one male marker band and two female marker bands were found in the male and female parental line, respectively. Based on the male marker band and female marker bands, the genetic purity of F1 seeds can easily be determined in the laboratory.