Abstract Background The defect of B cell self-tolerance and the continuous antigen presentation by T cells (TCs) mediated by autoreactive B cells (BCs) play a key role in the occurrence and development of systemic lupus erythematosus (SLE). PD-1/PD-L1 signaling axis negatively regulates the immune response of TCs after activation and maintains immune tolerance. However, the effect of PD-1/PD-L1 signaling axis on the interaction between CD19+B/CD4+TCs in the peripheral blood of patients with SLE has not been studied in detail. Methods PD-1/PD-L1 and Ki-67 levels in peripheral blood (PB) of 50 SLE patients and 41 healthy controls (HCs) were detected through flow cytometry, and then the expression of PD-1+/−cells and PD-L1+/−cells Ki-67 was further analyzed. CD19+B/CD4+TCs were separated for cell culture and the supernatant was collected to determine proliferation and differentiation of TCs. IL-10 and IFN-γ secretion in the supernatant was also determined using ELISA. Results The PD-1, PD-L1, and Ki-67 levels on CD19+B/CD4+TCs in patients with SLE were higher than HCs. In CD19+B/CD4+TCs of SLE patients, the proliferative activity of PD-L1+ cells was higher than that of PD-L1− cells, and the proliferative activity of PD-1+ cells was higher than that of PD-1− cells. In the system co-culturing CD19+B/CD4+TCs from HCs/SLE patients, activated BCs promoted TCs proliferation and PD-L1 expression among TCs. Addition of anti-PD-L1 to co-culture system restored the proliferation of TCs, and inhibited IL-10/IFN-γ level. The addition of anti-PD-L1 to co-culture system also restored Tfh and downregulated Treg in HCs. Conclusions Axis of PD-1/PD-L1 on CD19+B/CD4+TCs in PB of SLE patients is abnormal, and cell proliferation is abnormal. In CD19+B/CD4+TCs of SLE patients, the proliferative activity of PD-L1+ and PD-1+ cells compared with PD-L1− and PD-1− cells in SLE patients, respectively. CD19+B/CD4+TCs in SLE patients can interact through PD-1/PD-L1. selftolerance self tolerance (TCs (BCs SLE. . (SLE) PD1/PDL1 PD1PDL1 PDPDL PD 1/PD L1 1 L PD-1/PD-L However CD19BCD4TCs CDBCDTCs CD19 CD4 CD detail Ki67 Ki 67 Ki-6 (PB 5 4 HCs (HCs cytometry PD1+/−cells PD1cells PDcells 1+/−cells PDL1+/−cells PDL1cells PDLcells L1+/−cells analyzed IL10 IL 10 IL-1 IFNγ IFN γ ELISA PD1, PD1 1, PD-1 PDL1, PDL1 PDL L1, PDL1+ L1+ PDL1− L1− PD1+ 1+ PD1− 1− coculturing co culturing HCsSLE PD-L antiPDL1 antiPDL anti anti-PD-L coculture IL10/IFNγ IL10IFNγ ILIFNγ 10/IFN level abnormal respectively PD1/PDL1. L1. (SLE PD1/PDL PD1PDL 1PD BCD CD1 Ki6 6 Ki- 1cells L1cells Lcells IL1 IL- PD- 10IFN

RESUMEN Introducción: La condición física de los futbolistas en las competiciones deportivas ha ido mejorando a lo largo de los años y el rendimiento óptimo de sus habilidades profesionales en condiciones competitivas se ha convertido en un factor esencial para la victoria en los partidos de fútbol. Objetivo: Este trabajo explora los efectos de diferentes métodos que emplean el entrenamiento de la fuerza en las técnicas de pateo de fútbol con el objetivo de encontrar el conjunto que mejor permita la precisión de los golpes a través de su estabilidad. Métodos: Se dividieron aleatoriamente 36 jugadores de fútbol, sin diferencias estadísticas en cuanto a la aptitud física y las características de capacidad integral de los jugadores, en grupo experimental y grupo de control. Ambos grupos de sujetos fueron entrenados durante 12 semanas, sólo el grupo experimental recibió la intervención especial de entrenamiento de fuerza para la estabilidad. Se midió la puntuación del pase de la pelota curva en el regateo de 20 metros antes y después del entrenamiento. Los datos fueron tratados estadísticamente. Resultados: La precisión de las patadas de los futbolistas del grupo experimental fue diferente a la de antes de la prueba (P<0,01). También hubo una diferencia significativa en la precisión de las patadas en el grupo de control (P<0,05). Las puntuaciones del regateo en arco de 20 metros en el grupo experimental fueron estadísticamente significativas en comparación con las anteriores a la prueba (P<0,05). No hubo diferencias significativas entre el grupo de control y las puntuaciones de la prueba en las puntuaciones de los pases de balón curvo en el regateo de 20 metros (P>0,05). Conclusión: Se han desarrollado métodos de fuerza funcional para lograr el objetivo de mejorar la precisión de las patadas en los atletas. Los entrenadores deben prestar atención a la preparación física, una actitud que anima a los jugadores a conseguir la fuerza física suficiente para los partidos de fútbol con sus habilidades de pateo. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción Objetivo estabilidad Métodos 3 1 semanas 2 Resultados P<0,01. P001 P P<0,01 . 0 01 (P<0,01) P<0,05. P005 P<0,05 05 (P<0,05) P>0,05. P>0,05 (P>0,05) Conclusión atletas II tratamiento P00 P<0,0 (P<0,01 (P<0,05 P>0,0 (P>0,05 P0 P<0, (P<0,0 P>0, (P>0,0 P<0 (P<0, P>0 (P>0, P< (P<0 P> (P>0 (P< (P> (P

ABSTRACT Introduction: The physical condition of soccer players in sports competitions has improved over the years. The optimal performance of their professional skills in competitive conditions has become essential for victory in soccer matches. Objective: This paper explores the effects of different methods employing strength training on soccer kicking techniques aiming at the set that best enables the accuracy of hits through its stability. Methods: 36 soccer players were randomly divided into the experimental and control groups, with no statistical difference in fitness and the comprehensive ability characteristics of the players. Both subjects were trained for 12 weeks; only the experimental group received the special strength training intervention for stability. The passing score of the curve ball in 20-meter dribbling was measured before and after training. The data were statistically treated. Results: The kicking accuracy of soccer players in the experimental group differed from before the test (P<0.01). There was also a significant difference in kicking accuracy in the control group (P<0.05). The 20-meter arc dribbling scores in the experimental group were statistically significant compared to those before the test (P<0.05). There was no significant difference between the control group and the test scores on curve ball passing scores in 20-meter dribbling (P>0.05). Conclusion: Functional strength methods to achieve the goal of improving kicking accuracy in athletes have been developed. Coaches should pay attention to physical training, an attitude that encourages players to achieve sufficient physical strength for soccer games with their kicking skills. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction years matches Objective stability Methods 3 groups 1 weeks 20meter meter 20 treated Results P<0.01. P001 P P<0.01 . 0 01 (P<0.01) P<0.05. P005 P<0.05 05 (P<0.05) P>0.05. P>0.05 (P>0.05) Conclusion developed II outcomes 2 P00 P<0.0 (P<0.01 (P<0.05 P>0.0 (P>0.05 P0 P<0. (P<0.0 P>0. (P>0.0 P<0 (P<0. P>0 (P>0. P< (P<0 P> (P>0 (P< (P> (P

RESUMO Introdução: A condição física dos jogadores de futebol nas competições esportivas tem se aprimorado ao longo dos anos e o desempenho ótimo de suas habilidades profissionais em condições competitivas tornou-se um fator essencial para a vitória nas partidas de futebol. Objetivo: Este artigo explora os efeitos de diferentes métodos empregando o treinamento de força sobre as técnicas de chute de futebol visando o conjunto que melhor capacita a precisão de acertos através de sua estabilidade. Métodos: 36 jogadores de futebol foram divididos aleatoriamente, sem diferença estatística de aptidão física e as características de capacidade abrangente dos jogadores, em grupo experimental e controle. Ambos os grupos de sujeitos foram treinados durante 12 semanas, somente o grupo experimental recebeu a intervenção especial de treinamento de força para estabilidade. A pontuação de passe da bola curva em drible de 20 metros foi medida antes e depois do treino. Os dados foram tratados estatisticamente. Resultados: A precisão do chute dos jogadores de futebol no grupo experimental foi diferente daquela antes do teste (P<0,01). Também houve uma diferença significativa na precisão de chutes no grupo de controle (P<0,05). As pontuações dos dribles de arco de 20 metros no grupo experimental foram estatisticamente significativas em comparação com aquelas antes do teste (P<0,05). Não houve diferença significativa entre o grupo de controle e os resultados do teste na pontuação de passe da bola curva em drible de 20 metros (P>0,05). Conclusão: Métodos de força funcional para alcançar o objetivo de melhorar a precisão de chute nos atletas foram desenvolvidos. Os treinadores devem prestar atenção ao treinamento físico, atitude que incentiva aos jogadores atingirem a força física suficiente para os jogos de futebol com suas habilidades de chute. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução tornouse tornou Objetivo estabilidade 3 aleatoriamente 1 semanas 2 treino Resultados P<0,01. P001 P P<0,01 . 0 01 (P<0,01) P<0,05. P005 P<0,05 05 (P<0,05) P>0,05. P>0,05 (P>0,05) Conclusão desenvolvidos físico II tratamento P00 P<0,0 (P<0,01 (P<0,05 P>0,0 (P>0,05 P0 P<0, (P<0,0 P>0, (P>0,0 P<0 (P<0, P>0 (P>0, P< (P<0 P> (P>0 (P< (P> (P

Abstract Under the influence of the COVID-19, people's awareness of physical health and immunity has increased significantly. Chitooligosaccharide is an oligomer of β-(1, 4)-linked D -glucosamine, furthermore, is one of the most widely studied immunomodulators. Chitooligosaccharide can be prepared from the chitin or chitosan polymers through enzymatically, chemically or physically processes. Chitooligosaccharide and its derivatives have been proven to have a wide range of biological activities including intestinal flora regulation, immunostimulant, anti-tumor, anti-obesity and anti-oxidation effects. This review summarizes the latest research of the preparation methods, biological activities in immunity and safety profiles of Chitooligosaccharide and its derivatives. We recapped the effect mechanisms of Chitooligosaccharide basing on overall immunity. Comparing the effects of Chitooligosaccharide with different molecular weights and degree of aggregation, a reference range for usage has been provided. This may provide a support for the application of Chitooligosaccharide in immune supplements and food. In addition, future research directions are also discussed. COVID19, COVID19 COVID 19, 19 COVID-19 peoples people s significantly β1, β1 β 1, 1 β-(1 4linked linked 4 glucosamine, glucosamine -glucosamine furthermore immunomodulators enzymatically processes regulation immunostimulant antitumor, antitumor anti tumor, tumor anti-tumor antiobesity obesity antioxidation oxidation methods aggregation provided food addition discussed COVID1 COVID-1 β-( COVID- β-

The findings of this article reveal that the development of the independent learning model in the American literature curriculum positively impacts teachers' job psychological satisfaction. This study highlights the contribution of autonomous learning mode in empowering students' subjective status and enhancing their initiative, consequently reducing teachers' psychological pressure and improving their overall satisfaction with their work. CONTRIBUTION: The results of this study hold implications for scholars in the field, particularly those engaged in practical theology and religious educational studies

ABSTRACT: Soybean cyst nematode (SCN, Heterodera glycines) is a chief plant–parasitic nematode of soybean. Application of synthetic chemical nematicides poses negative side effects to human health and the environment. Therefore, the search for a safe and effective approach is more relevant. This study evaluated the effects of Genistein and Daidzein on the physiological index of soybean cyst nematodes, individual morphology, reversal frequency, respiration, and body fluid leaking of second–stage juveniles (J2s) of soybean cyst nematode. The results showed that body length of J2s decreased, while stylet and tail transparent area elongated. Additionally, after the treatment, the body became hollow and shrunken, the J2s stiffened, whereas the reversal frequency decreased dramatically after 24 h of treatment. Moreover, the body fluid leakage was intensified and respiration was inhibited. Oxygen consumption decreased by 86.7 % and 70.1 %, while, in contrast, electrical conductivity increased by 40.1 % and 36 % at 100 μg mL−1 of Genistein and Daidzein, respectively, after 48 h of exposure. The smaller number of J2s in soybean roots, the slower development rate, and the abnormal sexual differentiation were found in greenhouse assay. Thus, Genistein and Daidzein, especially Genistein, have strong effects on the physiological index of soybean cyst nematodes. Moreover, the effects were time and dosage–dependent.

OBJECTIVES: TTP488, an antagonist of the receptor for advanced glycation end-products, was evaluated as a potential treatment for patients with mild-to-moderate Alzheimer’s disease (AD). However, the mechanism underlying the protective action of TTP488 against AD has not yet been fully explored. METHODS: Healthy male rats were exposed to aberrant amyloid β (Aβ) 1-42. Lipopolysaccharide (LPS) and the NOD-like receptor family pyrin domain containing 1 (NLRP1) overexpression lentivirus were injected to activate the NLRP1 inflammasome and exacerbate AD. TTP488 was administered to reverse AD injury. Finally, tofacitinib and fludarabine were used to inhibit the activity of Janus tyrosine kinase (JAK) and signal transducer and activator of transcription (STAT) to prove the relationship between the JAK/STAT signaling pathway and TTP488. RESULTS: LPS and NLRP1 overexpression significantly increased the NLRP1 levels, reduced neurological function, and aggravated neuronal damage, as demonstrated by the impact latency time of, time spent by, and length of the platform covered by, the mice in the Morris water maze assay, Nissl staining, and immunofluorescence staining in rats with AD. CONCLUSIONS: TTP488 administration successfully reduced AD injury and reversed the aforementioned processes. Additionally, tofacitinib and fludarabine administration could further reverse AD injury after the TTP488 intervention. These results suggest a new potential mechanism underlying the TTP488-mediated alleviation of AD injury.

The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with different expression profiles. Moreover, EBR25 induction had a greater influence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (> 5- fold up-regulation) than on psy, pds, bkt; EBR50 treatment had a greater effect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on ipi-1 and psy. Furthermore, astaxanthin biosynthesis under EBR was up-regulated mainly by ipi1־ and psy at the post-transcriptional level, pds, lyc, crtR-B, bkt and crtO at the transcriptional level and ipi-2 at both levels.

The use of the filamentous fungus, Ashbya gossypii, to improve riboflavin production at an industrial scale is described in this paper. A riboflavin overproducing strain was isolated by ultraviolet irradiation. Ten minutes after spore suspensions of A. gossypii were irradiated by ultraviolet light, a survival rate of 5.5% spores was observed, with 10% of the surviving spores giving rise to riboflavin-overproducing mutants. At this time point, a stable mutant of the wild strain was isolated. Riboflavin production of the mutant was two fold higher than that of the wild strain in flask culture. When the mutant was growing on the optimized medium, maximum riboflavin production could reach 6.38 g/l. It has even greater promise to increase its riboflavin production through dynamic analysis of its growth phase parameters, and riboflavin production could reach 8.12 g/l with pH was adjusted to the range of 6.0-7.0 using KH2PO4 in the later growth phase. This mutant has the potential to be used for industrial scale riboflavin production.

Ionic liquids based on 1, 3-dialkylimidazolium cation and pyridinium cation were employed as phase transfer catalysts for the phase transfer synthesis of dichlorocarbene and mixed ether. The results showed that ionic liquids based on 1, 3-dialkylimidazolium cation were efficient and reusable