ABSTRACT The objectives were to evaluate the effects of monensin and virginiamycin, alone or combined, on supplemented Nellore cattle grazing tropical grass during the rainy season. Two experiments were conducted simultaneously to evaluate intake, digestibility, CH4 emissions, blood parameters, performance, and carcass characteristics (Exp. 1), and ruminal fermentation and relative abundance of ruminal microorganisms (Exp. 2). Animals (n = 92 Exp. 1 and n = 12 Exp. 2) were distributed in a completely randomized design and allocated in twelve paddocks composed of Urochloa brizantha (A. Rich.) Stapf. cv. Xaraés. A protein-energetic supplementation of 3 g/kg of BW per day was provided to all animals. Supplements were: without additives (WA), monensin alone at 80 mg/kg of product (MN), virginiamycin alone at 150 mg/kg of product (VM), and monensin (80 mg/kg of product) combined with virginiamycin (150 mg/kg of product; MNVM). Treatments did not affect intakes of total dry matter (DM), supplement DM, and nutrients. However, the intakes of forage DM and crude protein decreased in cattle fed MNVM compared with animals fed WA, MN, and VM. Total volatile fatty acids increased in animals fed VM. Ruminal NH3-N decreased, and pH increased in animals fed MN, VM, and MNVM. Relative abundance of total F. succinogenes and S. ruminantium decreased and R. flavefaciens increased in animals fed MN and VM at d 118. Treatments had no effect on enteric CH4 emissions. The average daily gain (ADG) and total gain were greater in cattle fed MNVM than in cattle fed MN. Combination of monensin and virginiamycin altered the rumen microbial populations but did not decrease enteric CH4 emissions. However, it decreased forage dry matter intake without altering the ADG and total weight gain, leading to an increase in feed efficiency. Results from this study indicate an advantage in including feed additives combined in the diet of supplemented Nellore cattle grazing tropical grass during the rainy season.

RESUMO. Doze novilhos canulados no rúmen (401,0 ± 41,5 kg) e 24 meses de idade foram usados em delineamento de quadrado latino truncado replicado com seis animais, seis tratamentos e quatro períodos, para avaliar o efeito da glicerina bruta (GB; 80,3% de glicerol) com amido ou ingredientes energéticos a base de fibra no concentrado sobre o CMS, digestibilidade da MS (DMS) e FDN (DFDN) e parâmetros ruminais. O período experimental foi 19 dias (14 dias de adaptação e 5 dias de coleta). As dietas foram: CO - sem glicerina bruta e milho como ingrediente do concentrado; GBM - GB (10% da MS) com milho no concentrado; GBCS - GB (10% da MS) com casca de soja no concentrado. As três dietas foram ofertadas em baixo (BC) ou alto (AC) teor de concentrado (TC; 40 ou 60%). Animais alimentados com BC ou AC apresentaram similar CMS, DMS e DFDN. Animais alimentados com GB com milho ou CS apresentaram maior concentração de propionato e menor relação A:P. Dietas com AC aumentou a concentração de propionato mas não afetou a DFDN. GB (10% da MS) pode ser usada para substituir o milho ou CS em dietas com 40 ou 60% de concentrado, sem afetar a DFDN.

ABSTRACT. Twelve ruminally cannulated steers (401.0 ± 41.5 kg) and 24 mo were used in a replicated arrangement truncated Latin Square with six animals in six treatments and four periods to evaluate the effect of crude glycerine (CG; 80.3% of glycerol) with starch or fiber-based energy ingredients in the concentrate on DMI, DM (DMD) and NDF digestibility (NDFD) and ruminal parameters. Experimental periods were 19 days (14 days for adaptation and 5 days to sampling). Diets were: CO - without CG and corn as ingredient of concentrate; CGC - inclusion of CG (10% of DM) with corn in the concentrate; and CGSH - inclusion of CG (10% of DM) with soybean hulls (SH) in the concentrate. All three diets were offered at low (LC) or high (HC) concentrate level, CL (40 or 60%). Animals fed LC or HC diets had similar DMI, DMD and NDFD. Animals fed diets with CG associated with corn or SH had higher propionate concentrations and lower A:P ratio. Diets with HC increase the propionate but do not affect the NDFD. CG (10% of DM) can be used to replace corn or SH in diets with 40 or 60% of concentrate, without affect NDFD.

Objetivou-se neste trabalho avaliar a qualidade da carne maturada de tourinhos Nelore, sob dois graus de acabamento (GA) de carcaça. Foram utilizados 14 animais, com aproximadamente 450 ± 30 kg de peso corporal. A espessura de gordura subcutânea (EGS) dos animais foi avaliada no início do período experimental. Foram escolhidos 14 animais com EGS de 0-3 mm e 3,1-6 mm, formando-se dois grupos ao final do experimento: animais com acabamento entre 3-6 mm (sete animais) e entre 6,1-10 mm (sete animais). A cada 28 dias foram avaliados a EGS entre a 12-13ª costelas com ajuda de unidade ultrassonográfica. Os valores de coloração da carne, pH, perdas por cocção e força de cisalhamento não tiveram efeito de interação entre os tratamentos (p > 0,05). Não houve diferença significativa entre o GA sobre o comprimento de sarcômero (p = 0,11). Houve interação entre GA e maturação sobre a capacidade de retenção de água (p < 0,01). Os graus de acabamento avaliados não interferem na qualidade da carne, mas carcaças com acabamento entre 6,1-10 mm apresentam maior capacidade de retenção de água. A maturação do músculo longissimus em até 14 dias melhora a maciez da carne.

This trial aimed to evaluate the quality of aged beef from Nellore young bulls under two yield grade (YG). Fourteen animals with approximately 450 ± 30 kg body weight were evaluated for backfat thickness (BFT) at the beginning of the experimental period. Seven animals had BFT of 0-3 mm and seven animals, 3.1-6 mm. Two groups were formed at the end of the experiment: animals finished with 3-6 mm BFT (seven animals) and animals finished with 6.1 to 10 mm BFT (seven animals). Every 28 days, we evaluated by ultrasound the BFT between the 12nd 13rd ribs. There was no interaction between YG and aging for beef color, pH, cooking losses and shear force (p > 0.05). There was no effect of YG on sarcomere length (p = 0.11). However, there was interaction between YG and aging on water holding capacity (p < 0.01). The yield grades evaluated did not interfere with meat quality, but carcasses with 6.1 - 10 mm of backfat thickness showed highest water holding capacity. The aging of the longissimus muscle for up to 14 days improves beef tenderness.