ABSTRACT The objective of this study was to determine the effects of different zilpaterol hydrochloride (ZH) levels and feeding methods on the growth performance and carcass traits of Japanese quails. In this trial, 224 male quails with 34 days of age were distributed into seven treatments. A factorial arrangement with three ZH levels (0.2, 0.225, and 0.25 mg zilpaterol/kg of live weight−1) × two feeding methods (ZH supplementation daily or every two days) plus a control treatment (no ZH supplementation) was applied. The weight gain of birds fed ZH every two days was significantly higher than those fed ZH daily during the period of 41-47 days (p=0.03). During the period of 34-40 days, ZH-supplemented birds presented higher weight gain (p<0.001) and better feed conversion ratio (p=0.005) compared with the control group. Moisture, protein, ash, and cholesterol levels of the combination of breast and leg were not affected (p≥0.10) by zilpaterol supplementation. Leg muscle fat and protein contents of control birds were higher and lower compared with ZH-supplemented birds, respectively (p=0.002 and p=0.05). In conclusion, ZH supplementation in the diet of male Japanese quail improved growth performance during the finishing period (34-40d), and the most economical level of ZH is 0.2 (mg/kg of LW−1 d−1), when supplemented every two days.

To clarify the effects of high (20 and 40% more than normal) and low (20% less than normal) daily feed allowance on egg and body parameters, ovarian morphology and plasma glucose, cholesterol, triacylglycerol, leptin-like hormone, nitrite/nitrate and gene expression of inducible nitric oxide synthase (iNOS) in the granulosa layer of F1 follicle, broiler breeder hens (30-week-old) were fed for 30 days. Egg and body parameters significantly changed between treatments (p<0.05). Effect of different level of feed intake on ovarian morphology parameters was significant (p<0.05), except for white follicles. After 30 days of experiment, plasma nitrite/nitrate (as a index of plasma nitric oxide) and leptin-like hormone increased in FI+20% and FI+40% groups as compared to controls (p<0.05). Plasma level of leptin-like hormone also significantly (p<0.05) increased in FI+40% group. The relative amount of iNOS mRNA expression in the granulosa layer of F1 follicles was significantly higher in FI-20% and FI+40% groups than in control group only after 4 weeks of experiment. The amount of these elevations in the FI-20% and FI+40% groups were 32.4% and 60.9% respectively. It was concluded that iNOS gene is normally expressed in follicular granulosa cells of F1 follicle of broiler breeder hens 2-4 hours before ovulation. However, over- and underfeeding of hens increased iNOS expression in F1 follicle, which may be one of the atresia-inducing factors in hierarchical follicles as shown by the significant (p<0.05) increase of shrunken follicles in the ovary of over- and under-fed broiler breeder hens after 30 days of feeding.