Resumo: O objetivo deste artigo é elaborar um panorama da literatura sobre Gestão de Projetos (GP) em Pequenas e Médias Empresas (PMEs) através da análise da evolução das publicações e principais temáticas entre 1996 e 2016 para motivar futuras pesquisas que auxiliem PMEs a usarem práticas de Gestão de Projetos de forma mais eficiente. O estudo foi conduzido por bibliometria associada à análise de conteúdo das publicações coletadas nas bases Web of Science e Scopus e no periódico International Journal of Project Management. Para tanto, os softwares VOSviewer, Nvivo, Minitab e Excel foram utilizados nas análises. A varredura de 235 publicações sobre Gestão de Projetos em PMEs permitiu traçar um panorama da literatura. Ademais, quatro categorias temáticas se destacaram: Práticas de Gestão de Projeto, Sistemas de Planejamento e Controle, Colaboração e Gestão de Conhecimento. Como características da gestão de projetos em PMEs, observa-se a necessidade de uma metodologia mais leve, flexível e orientada a pessoas. Adicionalmente, os resultados apontam que os principais desafios envolvem a falta de recursos e pessoal qualificado, além da alta rotatividade. Entretanto, superando essas questões, a GP pode beneficiar o crescimento e a inovação em PMEs. Portanto, o estudo apresenta um quadro conceitual de benefícios e desafios da gestão de projetos em PMEs, reduzindo a lacuna de pesquisa no tema. Além disso, são apresentadas recomendações para pesquisas futuras, principalmente no Brasil.

Abstract: The purpose of this paper is to design an overview about Project Management (PM) in Small and Medium-Sized Enterprises (SMEs) by analysing the evolution of publications and the main topics since 1996 to 2016 to motivate future research that helps SMEs to apply PM practices more efficiently. This study performed bibliometrics associated with content analysis of publications collected in scientific bases Web of Science and Scopus and in the periodic International Journal of Project Management. For that, the software VOSviewer, Nvivo, Minitab, and Excel were used in the analyses. The scan of 235 papers about Project Management in SMEs supported a literature overview. Furthermore, four thematic categories are highlighted: Project Management Practices, Planning and Control Systems, Collaboration, and Knowledge Management. Moreover, it was observed that SMEs requires a lighter PM methodology, focused on people and flexible. Besides that, the results show that the main challenges involve a lack of resources and qualified people and the high turnover. However, overcoming these issues, PM can benefit growth and innovation in SMEs. Therefore, this study presents a conceptual framework of benefits and challenges in Project Management in SMEs, reducing the research gap. Furthermore, recommendations for future research, mainly in Brazil, are given.

Abstract Baby Hamster Kidney cells (BHK-21) are commonly used in research and the biopharmaceutical industry. This work aimed to model the kinetic performance in batch operation mode of BHK-21 cells cultured in two stirred tank configurations using different dissolved oxygen concentrations and pH control strategies. Viable and dead cell concentrations, as well as glucose, glutamine, lactate and ammonium concentrations, were monitored. Statistical multiple linear regression, logistic equation and multiplicative Monod kinetic models were fitted. Statistical models for viable cells concentration as a function of nutrient and metabolite concentrations were significant (R2 >0.91). Logistic model parameters: intrinsic growth rate, cell density level in the medium and time for reaching maximum cell concentrations were within 0.061-0.083 h-1, 1.85-5.39 x 109 cell L-1 and 52-90 h ranges, respectively. A Monod-type model was satisfactorily fitted to the experimental data. Relative errors were lower than 10% for six monitored state variables in most of the assessed experimental conditions. The three models developed in this work can be used in bioprocesses involving BHK-21 with good fitting.

The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.