ABSTRACT Chenopodium quinoa Willd (quinoa) is a pseudo cereal that has been gaining prominence in production in several regions, due to increased demand in the consumer market. In the search to reduce productivity losses caused by pathogens, this work aimed to evaluate the cytogenotoxic potential of aqueous extracts of Syzygium aromaticum L., a possible alternative treatment for quinoa seeds. The seeds were treated with extracts produced from S. aromaticum flowers buds at 2, 4, 6 and 8%. A water control was included. Germination, first germination count, germination speed index, emergence and sanity were evaluated. The design was completely randomized, composed of 100 seeds in five treatments. The incidence of fungi was quantified and subsequently, the cytogenotoxicity test was performed on Allium cepa. It was observed that the treatment of quinoa seeds with aqueous extract of S. aromaticum only affected germination and vigor at the highest concentration used (8%) and reduced the incidence of fungi. A negative effect on cell division was demonstrated but no genotoxic effect. Used at 4% (m / v) it could be an alternative for the treatment of quinoa seeds.

RESUMEN Chenopodium quinoa Willd (quinoa) es un pseudo cereal que ha ido ganando protagonismo productivo en varias regiones debido al aumento de la demanda del mercado de consumo. En la búsqueda de la reducción de las pérdidas de productividad causadas por patógenos, este trabajo tuvo como objetivo evaluar el potencial citogenotóxico de extractos acuosos de Syzygium aromaticum, posible tratamiento alternativo de semillas de quinua. Las semillas fueron tratadas con extractos producidos a partir de flores de S. aromaticum al 2, 4, 6 y 8%. Se incluyó un control con agua. Se evaluó la germinación, primer recuento de germinación, índice de velocidad de germinación, emergencia y sanidad. El diseño fue completamente aleatorizado, compuesto por 100 semillas en cinco tratamientos. Se cuantificó la incidencia de hongos y posteriormente, se realizó la prueba de citogenotoxicidad en Allium cepa. Se observó que el tratamiento de semillas de quinua con extracto acuoso de S. aromaticum solo afectó la germinación y el vigor a la mayor concentración empleada (8%) y redujo la incidencia de hongos. Se demostró un efecto negativo sobre la división celular pero no efecto genotóxico. Empleado al 4% (m/v) podría ser una alternativa para el tratamiento de semillas de quinoa.

Resumo Introdução: Estudos apontam para a efetividade de ações inclusivas relacionadas aos processos escolares, assim como a necessidade de formação especializada para terapeutas ocupacionais, capacitando-os para abordagens e ações colaborativas. Objetivos: Identificar ações desenvolvidas por terapeutas ocupacionais do Estado do Paraná no processo de inclusão escolar de crianças e adolescentes com necessidades educacionais especiais, e refletir sobre o contexto da educação inclusiva, a partir de perspectivas apresentadas. Método: Realizou-se levantamento de dados (survey), com a participação de 18 terapeutas ocupacionais. Aplicou-se questionário relacionado a inclusão escolar e atuação do profissional, com divulgação através de plataforma online e rede social. Submeteram-se os dados coletados à análise qualitativa, com análises exploratórias descritivas nas questões objetivas e de conteúdo, nas descritivas. Resultados: O principal envolvimento dos participantes com a inclusão escolar ocorre a partir de atendimento em ambiente clínico, porém 50% atendem, também, na escola regular. As principais formas de contribuição ocorrem através de avaliação, planejamento e desenvolvimento de atividades relacionadas a processos de inclusão, e de orientações às escolas. Destacam-se a participação da família e de outros profissionais envolvidos, em uma perspectiva colaborativa, e a falta de preparo da escola e dos professores para a efetivação dos processos de inclusão. Conclusão: Os resultados do estudo reafirmam a relevância da contribuição do terapeuta ocupacional para o enfrentamento dos desafios e a concretização da inclusão escolar. Enfatizam-se a necessidade de maior inserção destes profissionais na escola comum do ensino regular e a importância de ampliar as reflexões e problematizações em relação à construção de um sistema educacional mais democrático.

Abstract Introduction: Studies show the effectiveness of inclusive actions in educational processes, as well as the need for specialized training of occupational therapists, enabling them for collaborative approaches and actions. Objective: To identify actions developed by occupational therapists in the process of school inclusion of children and teenagers with special educational needs, and think over the context of inclusive education from the presented perspectives. Method: A survey with 18 occupational therapists for data collection. A questionnaire related to school inclusion and professional practice was applied through an on-line platform and a social network. Data gathered were forwarded to qualitative analysis, with descriptive, exploratory analysis for objective questions, and content analysis for the descriptives. Results: The main participants’ involvement in school inclusion occurred from their office practice, but 50% of them also assist in regular school. The main method of contribution occurred through assessment, planning and developing inclusion and guidance activities in schools. The participation of family and other professionals in a collaborative approach, the school and teachers lack of preparation to complete the inclusion processes were noteworthy. Conclusion: Study results reassure the importance of occupational therapist’s contribution to cope with the challenges and effectiveness of school inclusion. Emphasis is given to the need for greater integration of those professionals in regular school and the importance of expanding the considerations and questions regarding the construction of a more democratic educational system.

ABSTRACT Tricyclazole is currently one of the fungicides recommended for the treatment of diseases in irrigated rice. However, there is relatively little information on its cytotoxic and genotoxic potential. The objective of this study was to evaluate the cytotoxicity and genotoxicity of rice crop water after apllication of the tricyclazole fungicide through the Allium cepa L. test. The rice crop water samplings were collected before and 1, 15 and 30 days after application of the fungicide in rice plant shoots. The Allium cepa roots were placed in contact with the rice crop water to check for possible chromosomal abnormalities and mitotic index of the bioindicators meristematic cells. The data obtained by the Allium cepa test indicates that the application of the tricyclazole fungicide leads to an increase in the genotoxic activity in the rice crop water, through the appearance of chromosomal abnormalities, without, however, causing significant effects on the mitotic index. The major chromosomal alterations observed were anaphasic and telophasic bridges and laggard chromosomes.

ABSTRACT The purpose of this study was to evaluate the antiproliferative and antigenotoxic activity of Sambucus australis Cham. & Schltdl. aqueous extracts on the cell cycle of Allium cepa L. as well as determine the phenolic compounds in such extracts. S. australis inflorescences and leaves of two accessions were used for aqueous extract preparation at concentrations: 0.003 g/ml and 0.012 g/ml. A. cepa bulbs were rooted in distilled water and, subsequently, placed in treatments for 24 hours. Rootlets were collected and fixed in modified Carnoy’s solution for 24 hours and kept. The squash technique was performed for slide preparation. Root tips were smashed and stained with 2% acetic orcein, and a total of 4000 cells per treatment were analyzed. The phenolic compounds were determined using high-performance liquid chromatography and data was analyzed using the Scott-Knott test. The results show that S. australis aqueous extracts have antiproliferative potential. Besides, the extracts prepared from S. australis leaves of both accessions at a concentration of 0.012 g/ml have shown antigenotoxic activity. The phytochemical analysis allowed us to determine the presence of flavonoids and phenolic acids, of which kaempferol and chrologenic acid were the most predominant compounds in the extracts from the inflorescences and leaves, respectively.

RESUMO: crianças com paralisia cerebral (PC) apresentam déficit motor e podem ter poucas vivências sensório-motoras, o que interfere em sua principal atividade: o brincar. O objetivo deste estudo foi identificar a percepção de pais e de terapeutas ocupacionais sobre o comportamento lúdico de crianças com PC. O estudo foi desenvolvido com 25 crianças com PC utilizando os instrumentos Entrevista Inicial com os Pais e a Avaliação do Comportamento Lúdico das crianças destinados a terapeutas ocupacionais. Os resultados apontaram um índice de concordância que variou de 8% a 93% quanto ao interesse geral da criança pelas outras pessoas; 88% a 100% na categoria interesse geral da criança pelo ambiente sensorial, de 84 a 96% na categoria interesses e capacidades lúdicas; e, de 68% a 100% na categoria características da atitude lúdica. Os instrumentos utilizados foram capazes de identificar o comportamento lúdico de crianças com PC e podem auxiliar na delimitação de objetivos e plano terapêutico com abordagem centrada no cliente. Com este estudo verificou-se que as crianças com PC, independentemente de sua gravidade motora, demonstram atitude, ação e interesse pelo brincar, condizendo com a proposta da abordagem do Modelo Lúdico.

ABSTRACT: Children with cerebral palsy (CP) present motor disability and may have fewer sensorimotor experiences, which may interfere in their main activity: playing. The purpose of this study was to identify the perceptions of parents and occupational therapists on the ludic behavior of children with CP. The study was carried out with 25 children with CP using Initial Interview with Parents and Assessment of Ludic Behavior aimed at occupational therapists. Results indicated an agreement rate ranging from 8% to 93%; regarding the child's General Interest to Other People; from 88% to 100% for Child's General Interest for the Sensorial Environment; from 84% to 96% in the category Ludic Interests and Skills; and from 68% to 100% in the category Characteristics of the Ludic Attitude. The instruments used were capable of identifying the children's ludic behavior and may help define the objectives and treatment plan with a patient-centered approach. This study showed that children with CP, regardless of their motor severity, demonstrate attitude, action and interest in playing, consistently to the Ludic Model.

Peltodon longipes é utilizada como estimulante e emenagoga. Objetivou-se realizar análises genotóxica e cromatográfica dos extratos de duas amostras de P. longipes, coletadas nos municípios de Santa Maria e Tupanciretã, RS, Brasil. O teste de Allium cepa foi utilizado para análise da genotoxicidade e a cromatografia líquida de alta eficiência, para determinação dos compostos fenólicos. O experimento de genotoxicidade constou de nove grupos de quatro bulbos de A. cepa. Os bulbos foram enraizados em água destilada e após transferidos para os tratamentos, por 24 horas, permanecendo o controle negativo em água. Os tratamentos foram: extratos aquosos nas concentrações de 5 e 15 g L-1 de cada amostra, além de quatro grupos tratados com glifosato 1%, um deles usado como controle positivo e outros três para testar a recuperação de danos ao DNA, utilizando água e os extratos de P. longipes da amostra de Santa Maria. Todos os extratos de P. longipes demonstraram potencial antiproliferativo, porém o efeito foi significativamente maior para os extratos da amostra de Tupanciretã. Essa amostra também apresentou maior quantidade de ácido rosmarínico e canferol, o que pode estar relacionado com os efeitos encontrados nesses extratos. Somente extratos da amostra de Santa Maria demonstraram potencial genotóxico.

Peltodon longipes is used as a stimulant and emmenagogue. The objective of this study was to perform genotoxic and chromatographic analyses of the extracts of two samples of P. longipes, collected from the cities of Santa Maria and Tupanciretã, RS, Brazil. The Allium cepa assay was used to analyze genotoxicity while high-performance liquid chromatography was employed to determine phenolic compounds. The genotoxicity experiment consisted of nine groups each comprising four A. cepa bulbs. Bulb roots were developed in distilled water and then transferred for the treatments, for 24 hours, and the negative control remained in water. The treatments were: aqueous extracts at concentrations of 5 and 15 g L-1 for each sample, plus four groups treated with 1% glyphosate, one of which was used as a positive control and the other three for testing DNA damage recovery using water and the extracts of P. longipes from Santa Maria. All extracts of P. longipes exhibited anti-proliferative potential, although the effect was significantly greater for the extracts from the Tupanciretã sample. This sample also contained the highest amount of rosmarinic acid and kaempferol, which may confer the effects found in these extracts. Only extracts from the Santa Maria sample exhibited genotoxic potential.

Punica granatum L., conhecida como romanzeira, é originária da Ásia e encontra-se distribuída por todo Brasil. É usada para o tratamento de doenças inflamatórias, infecciosas e respiratórias. Em decorrência da grande utilização de recursos fitoterápicos, é necessário esclarecer à população sobre a grande quantidade de substâncias existentes nas plantas e sobre os benefícios e prejuízos de tais substâncias à saúde. O presente trabalho objetivou realizar a análise cromatográfica e o estudo da genotoxicidade dos extratos aquosos das cascas dos frutos de P. granatum através do teste de Allium cepa L. Para a montagem do experimento, foram utilizados 7 tratamentos: T1-água destilada, T2-chá 5 g.L-1, T3-chá 10 g.L-1, T4-glifosato a 9,6%, T5-glifosato para recuperação em água destilada, T6-glifosato para recuperação em chá 5 g.L-1 e T7-glifosato para recuperação em chá 10 g.L-1. As radículas foram coletadas e fixadas em etanol:ácido acético (3:1) por 24 horas, e armazenadas em álcool 70%, sob refrigeração. Realizou-se análise por cromatografia líquida de alta eficiência para quantificação dos compostos fenólicos. Nos extratos de P. granatum foram observados em maior quantidade: ácido gálico, catequina, ácido cafeico e rutina. Além disso, os extratos demonstraram potencial antiproliferativo, sem apresentar atividade antimutagênica e genotóxica.

Punica granatum L., locally known as romanzeira, is native to Asia but found throughout Brazil. P. granatum is used for treating inflammatory, infectious and respiratory diseases. The aim of this study was to evaluate the chromatography and genotoxicity of an aqueous extract of P. granatum (pomegranate) fruit peel using the Allium cepa L. test. The experiment set-up entailed 7 treatments: T1-distilled water, T2-tea 5 g.L-1, T3-tea 10 g.L-1, T4-glyphosate at 9.6%, T5-glyphosate with subsequent recovery in distilled water, T6-glyphosate with subsequent recovery in tea 5 g.L-1 and T7-glyphosate with subsequent recovery in tea 10 g.L-1. The rootlets were collected and fixed in ethanol:acetic acid (3:1) for 24 hours, then stored in 70% ethanol under refrigeration. Analysis was performed using high performance liquid chromatography for the quantification of the extracted phenolic compounds. Gallic acid, catechin, caffeic acid, and rutin were abundant in the extracts of P. granatum. The extracts were found to exhibit antiproliferative potential but not antimutagenic or genotoxic activity.

Inúmeros estudos têm sido desenvolvidos visando entender a fisiologia da deterioração de sementes, entretanto, as etapas iniciais desse processo ainda não estão completamente elucidadas. A perda do vigor e da viabilidade das sementes também têm sido associada aos danos no material genético. Este estudo tem por objetivo avaliar alterações citogenéticas e fisiológicas no processo de deterioração de sementes de trigo , submetidas ao envelhecimento artificial. Duas cultivares foram utilizadas, Ônix e Fundacep Campo Real, submetidas a seis tratamentos: controle (T1), 24 (T2), 48 (T3), 72 (T4), 96 (T5) e 120 (T6) horas, sob envelhecimento artificial a 42 °C e aproximadamente 100% de UR. Foram efetuados os testes de germinação e vigor (Primeira Contagem, Condutividade Elétrica, Emergência de Plântulas e Indice de Velocidade de Emergência). Avaliações citogenéticas foram realizadas em tecidos de pontas de radículas. Determinou-se o número de células em divisão e o índice mitótico. O número de células em divisão e o índice mitótico foram afetados em função da deterioração induzida, pelo envelhecimento artificial. Menor número de células em divisão foi observado em sementes submetidas ao envelhecimento por períodos prolongados. Foram detectadas anormalidades cromossômicas em células mitóticas de sementes envelhecidas artificialmente, como ocorrência de micronúcleos, quebras e pontes cromossômicas.

Several studies have been developed to understand the physiology of seed deterioration, however, the initial steps of the deterioration process are not completely clear. Seed vigor and viability losses are also associated with genetic material damage. This study was carried out to detect cytogenetic and physiological alterations occurring during the deterioration process of wheat seeds, due to artificial aging stress. Two cultivars, Ônix and Fundacep Campo Real, were submitted to six different treatments: control (T1), 24 (T2), 48 (T3), 72 (T4), 96 (T5) and 120 (T6) hours under artificial aging conditions: 42 °C and 100% relative humidity. Germination and vigor tests (First Count of Germination, Electrical Conductivity, Seedling Emergence in the Field and Emergence Speed Index) were performed. Cytogenetic evaluations were performed through smear technique for root tips, stained with 2% acetic orcein. Total number of cells under division and mitotic index (MI) were determined. According to results, vigor and mitotic index were affected due to deterioration process induced by artificial aging stress. A reduced number of dividing cells were observed in seeds submitted to prolonged period of aging. Chromosomal abnormalities in mitotic cells of aged seeds were detected with the occurrence of micronuclei, chromosome breaks and bridges.

Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied. Results showed that glyphosate and the extracts of both P. brachypoda and P. birotula reduced the mitotic index as compared with the negative control (distilled water). Surprisingly, however, both extracts from P. brachypoda and P. birotula caused a partial reversal of the antiproliferative effect of glyphosate when used as a post-treatment. Glyphosate also induced the highest number of cells with chromosomal alterations, which was followed by that of P. birotula extracts. However, the extracts from P. brachypoda did not show any significant genotoxic effect. Post-treatment of glyphosate-treated samples with distilled water allowed a partial recovery of the genotoxic effect of glyphosate, and some of the Psychotria extracts also did so. Notably, post-treatment of glyphosate-treated samples with P. brachypoda extracts induced a statistically significant apoptotic effect. It is concluded that P. brachypoda extracts show antiproliferative effects and are not genotoxic, while extracts of P. birotula show a less potent antiproliferative effect and may induce chromosomal abnormalities. The finding of a partial reversion of the effects of glyphosate by a post-treatment with extracts from both plants should be followed up.

Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as 'barba-de-São-João', 'barba-de-bode', 'vassourinha branca', and 'mimosa'. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander's stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) for most accessions of P. paniculata using the Alexander's stain, which proved the most adequate method to estimate pollen viability.

Studies on pollen viability are useful for breeding Crotalaria juncea L. since they provide information that will increase the chance of successful intraspecific crossing. The aim of this study was to evaluate pollen viability in C. juncea accessions cultivated in Rio Grande do Sul, Brazil. Inflorescences of 10 accessions of C. juncea were collected, fixed in absolute ethanol: glacial acetic acid (3:1) for 72 h at room temperature and placed in 70 % ethanol. Analysis of pollen viability was performed with slides of anthers using the squashing technique and staining with acetic orcein 2 % and Alexanders stain. Means were compared using the Tukey test (p≤0.05). Pollen viability was high and significantly different among the accessions; the highest value was 99.67 % and the lowest 45.34 %. However, unviable pollen grains were also observed in all accessions.

Los estudios de la viabilidad del polen son útiles para el mejoramiento genético de Crotalaria juncea L., porque proveen información que aumentará la oportunidad de cruces intraespecíficos exitosos. El objetivo de este estudio fue evaluar la viabilidad del polen de accesiones de C. juncea cultivadas en Rio Grande do Sul, Brasil. Las inflorescencias de 10 accesiones de C. juncea fueron recolectadas, fijadas en etanol absoluto: ácido acético glacial (3:1) por 72 h a temperatura ambiente, y colocadas en etanol al 70 %. El análisis de viabilidad del polen se realizó en rebanadas de anteros mediante la técnica de aplastado y teñidas con orceina acética al 2 % y tinción de Alexander. Las medias se compararon con la prueba de Tukey (p≤ 0.05). La viabilidad del polen fue alta con diferencias significativas entre las accesiones; el valor más alto fue 99.67 % y el menor 45.34 %. Sin embargo, en todas las accesiones se observaron granos de polen no viable.

OBJECTIVE: To identify the perceptions of professional nurses on group interventions by occupational therapists in mental health with patients admitted to a university hospital. METHODS: A qualitative study of the type described. The notes of the coordinators of occupational therapy groups were analyzed in relation to the interviews held with a professional and technical nursing staff that participated in the occupational therapy intervention group for a period of two years. The results were analyzed using the collective subject discourse (DSC) method. RESULTS: We identified four central ideas of the DSC: occupational therapy as favoring integrated care, valuing the subject and his experience, the occupational therapy process to help and care for their own team, the occupational therapy group as a space of resonance and facilitation of the management with the patient because of the perception of relational aspects. CONCLUSION: The strategies were perceived, either as promoters of the reorganization of the life situation experienced by the patient on admission, or as learning opportunities and support for nursing staff.

OBJETIVO: Conocer las percepciones de profesionales de enfermería sobre intervenciones grupales de Terapia Ocupacional en Salud Mental realizadas con pacientes internados en un hospital universitario. MÉTODOS: Estudio de abordaje cualitativo del tipo descrito. Las anotaciones de las coordinadoras de los grupos de Terapia Ocupacional fueron analizadas en relación a las entrevistas abiertas junto a n enfermeras y n técnicos de enfermería que participaron de las intervenciones grupales de Terapia Ocupacional por el período de dos años. Las respuestas fueron sometidas al análisis de Discurso del Sujeto Colectivo (DSC). RESULTADOS: Se identificaron cuatro ideas centrales del DSC: la Terapia Ocupacional como favorecedora del cuidado integrado, valorizando al sujeto y su experiencia; la terapia ocupacional como proceso de ayuda y cuidado para el equipo; el grupo de Terapia Ocupacional como espacio de resonancia y facilitación en el manejo del paciente en razón de la percepción de aspectos relacionales. CONCLUSIÓN: Las estrategias fueron percibidas, tanto como promotoras de la reorganización de la situación vivida por el paciente en el internamiento, así como oportunidades de enseñanza y apoyo para el equipo de enfermería.

OBJETIVO: Conhecer as percepções de profissionais de enfermagem sobre intervenções grupais de Terapia Ocupacional em Saúde Mental realizadas com pacientes internados em um hospital universitário. MÉTODOS: Estudo de abordagem qualitativa do tipo descrito. As anotações das coordenadoras dos grupos de Terapia Ocupacional foram analisadas em relação às entrevistas abertas junto a n enfermeiras e n técnicos de enfermagem que participaram das intervenções grupais de Terapia Ocupacional pelo período de dois anos. As respostas foram submetidas à análise do Discurso do Sujeito Coletivo (DSC). RESULTADOS: Foram identificadas quatro ideias centrais do DSC: a Terapia Ocupacional como favorecedora do cuidado integrado, valorizando o sujeito e sua experiência; a terapia ocupacional como processo de ajuda e cuidado para a própria equipe; o grupo de Terapia Ocupacional como espaço de ressonância e facilitação no manejo com o paciente em razão da percepção de aspectos relacionais. CONCLUSÃO: As estratégias foram percebidas, quer como promotoras da reorganização da situação vivida pelo paciente na internação, quer como oportunidades de ensino e apoio para a equipe de enfermagem.

OBJETIVO: Conhecer as percepções de profissionais de enfermagem sobre intervenções grupais de Terapia Ocupacional em Saúde Mental realizadas com pacientes internados em um hospital universitário. MÉTODOS: Estudo de abordagem qualitativa do tipo descrito. As anotações das coordenadoras dos grupos de Terapia Ocupacional foram analisadas em relação às entrevistas abertas junto a n enfermeiras e n técnicos de enfermagem que participaram das intervenções grupais de Terapia Ocupacional pelo período de dois anos. As respostas foram submetidas à análise do Discurso do Sujeito Coletivo (DSC). RESULTADOS: Foram identificadas quatro ideias centrais do DSC: a Terapia Ocupacional como favorecedora do cuidado integrado, valorizando o sujeito e sua experiência; a terapia ocupacional como processo de ajuda e cuidado para a própria equipe; o grupo de Terapia Ocupacional como espaço de ressonância e facilitação no manejo com o paciente em razão da percepção de aspectos relacionais. CONCLUSÃO: As estratégias foram percebidas, quer como promotoras da reorganização da situação vivida pelo paciente na internação, quer como oportunidades de ensino e apoio para a equipe de enfermagem.

OBJECTIVE: To identify the perceptions of professional nurses on group interventions by occupational therapists in mental health with patients admitted to a university hospital. METHODS: A qualitative study of the type described. The notes of the coordinators of occupational therapy groups were analyzed in relation to the interviews held with a professional and technical nursing staff that participated in the occupational therapy intervention group for a period of two years. The results were analyzed using the collective subject discourse (DSC) method. RESULTS: We identified four central ideas of the DSC: occupational therapy as favoring integrated care, valuing the subject and his experience, the occupational therapy process to help and care for their own team, the occupational therapy group as a space of resonance and facilitation of the management with the patient because of the perception of relational aspects. CONCLUSION: The strategies were perceived, either as promoters of the reorganization of the life situation experienced by the patient on admission, or as learning opportunities and support for nursing staff.

OBJETIVO: Conocer las percepciones de profesionales de enfermería sobre intervenciones grupales de Terapia Ocupacional en Salud Mental realizadas con pacientes internados en un hospital universitario. MÉTODOS: Estudio de abordaje cualitativo del tipo descrito. Las anotaciones de las coordinadoras de los grupos de Terapia Ocupacional fueron analizadas en relación a las entrevistas abiertas junto a n enfermeras y n técnicos de enfermería que participaron de las intervenciones grupales de Terapia Ocupacional por el período de dos años. Las respuestas fueron sometidas al análisis de Discurso del Sujeto Colectivo (DSC). RESULTADOS: Se identificaron cuatro ideas centrales del DSC: la Terapia Ocupacional como favorecedora del cuidado integrado, valorizando al sujeto y su experiencia; la terapia ocupacional como proceso de ayuda y cuidado para el equipo; el grupo de Terapia Ocupacional como espacio de resonancia y facilitación en el manejo del paciente en razón de la percepción de aspectos relacionales. CONCLUSIÓN: Las estrategias fueron percibidas, tanto como promotoras de la reorganización de la situación vivida por el paciente en el internamiento, así como oportunidades de enseñanza y apoyo para el equipo de enfermería.

Mikania glomerata is a plant used in Brazilian traditional medicine, known as ‘guaco'. It possesses anti-inflammatory properties and the aqueous extracts of its leaves are indicated for the treatment of diseases of the respiratory tract. This study aimed at evaluating the antiproliferative and genotoxic effect of Mikania glomerata leaf infusions on the cell cycle of onion. The material used was collected in the native environment from Rio Grande do Sul State, Brazil. Aqueous extracts through infusions were prepared in two concentrations: 4g/L (usual concentration) and 16g/L (4x more concentrated) of each of the populations. Two groups of four onion bulbs for each plant population were used plus a control group. The rootlets were fixed in ethanol-acetic acid (3:1), conserved in ethanol 70% and slides were prepared using the squashing technique colored with orcein 2%. The cells were observed and analyzed during cell cycle. Per group of bulbs, 2000 cells were analyzed, and the mean values of the cell number of each of the phases of the cell cycle were calculated, determining the mitotic index (MI). Statistic analyses of the data were carried out by the x² (p= 0.05) test. We conclude that M. glomerata presents both antiproliferative and genotoxic activity.

Avaliou-se o efeito antiproliferativo de infusões de Pluchea sagittalis usando o teste de Allium cepa. Foram usadas infusões em três concentrações (2,5, 5 e 25g dm-3) de folhas cultivadas em três ambientes (in vitro, sala de crescimento climatizada e em campo). Foram usados seis grupos de bulbos para cada um dos 8 tratamentos e o os índices mitóticos foram obtidos a partir de 6000 células por tratamento. Concluiu-se que a infusão de folhas de P. sagittalis cultivadas em campo possui grande atividade antiproliferativa, bem como o sistema de cultivo de plantas influencia o potencial antiproliferativo.

We evaluated the antiproliferative effect of infusions from Pluchea sagittalis using the Allium cepa test. Infusions in three concentrations (2.5, 5, and 25 g dm-3) of leaves cultivated in three environments (in vitro, acclimatized growth chamber, and field) were used. Six onion bulbs were used for each of the eight treatments, and the mitotic index was obtained from 6000 cells per treatment. In conclusion, leaf infusions of P. sagittalis cultivated in the field have a high antiproliferative activity, as well as the cultivation system influences the antiproliferative potential.