OBJECTIVE: We investigated the influence of resistance training on body composition and matrix metalloproteinase 2 activity in skeletal muscles of rats fed a high-fat diet. METHODS: Thirty-two Wistar rats were divided into four experimental groups (n = 8/each) according to diet and exercise status: Control (standard diet), Obese Control (high-fat diet), Resistance Training (standard diet) and Obese Resistance Training (high-fat diet) groups. Animals were fed a high-fat diet for 12 weeks to promote excessive weight gain. Resistance Training groups performed 12 weeks of training periods after this period in a vertical ladder three times/week. Fat percentage, fat-free mass and fat mass were assessed using dual-energy X-ray absorptiometry, and matrix metalloproteinase 2 activity in biceps and gastrocnemius muscles was analyzed using zymography. RESULTS: Resistance training significantly reduced body and fat masses and fat percentages in both trained groups (p<0.05). The maximal carrying load between trained groups was not different, but relative force was higher in the Resistance Training group (p<0.05). Of note, increased matrix metalloproteinase 2 activity was noted in the tested muscles of both trained groups (p<0.05). CONCLUSION: In conclusion, altered body composition and muscle matrix metalloproteinase 2 activity promoted by excessive weight gain were positively modified by resistance training.

INTRODUÇÃO: Muitos trabalhos têm estudado o comportamento hormonal nos exercício resistido, entretanto poucos relacionam os hormônios cortisol, GH e insulina. OBJETIVO: Estudar os ajustes das concentrações plasmáticas dos hormônios cortisol, GH e insulina em exercícios resistidos de mesma intensidade com relação à massas musculares distintas. MÉTODOS: Dez voluntários, com 20,3 ± 4,2 anos, 74,1 ± 10,2 kg de peso, 177,2 ± 4,6 cm de estatura e 23,8 ± 3,2 kg/m2 de IMC, realizaram uma sessão de leg press (LP) e supino reto (SR) com quatro séries com 10 repetições a 70% 1 RM com três minutos de intervalo. Foram coletadas amostras de sangue para dosagem das concentrações plasmáticas de cortisol, GH e insulina em repouso (Pré) e em 0' (Rec. 0'), 30' (Rec. 30') e 90' (Rec. 90') de recuperação. RESULTADOS: As concentrações plasmáticas de cortisol foram significativamente reduzidas ao final da recuperação em LP (2,20±0,37 ng/dl para 1,33±0,38ng/dl) em relação à pré-dosagem. As concentrações de GH e insulina elevaram-se significativamente durante a recuperação. GH em LP foi significativamente maior em Rec. 0' (2,75±3,29 ng/ml para 9,60±5,32 ng/dl) do que em pré. A insulina elevou-se significativamente em Rec. 30' em LP (14,70±7,92 ulU/ml para 21,66 ± 8,61 ulU/ml) e em SR (6,17 ± 2,99 ulU/ml para 19,70 ± 13,8 ulU/ml) em relação à pré. As concentrações plasmáticas de insulina pré em LP foram significativamente superiores a SR (14,70 ulU/ml e 6,17 ± 2,99 ulU/ml). CONCLUSÃO: O exercício resistido promoveu diferentes ajustes nas concentrações hormonais de cortisol, GH e insulina durante o período de recuperação.

INTRODUCTION: Many works have studied the hormonal behavior in resistance exercise, however, few relate the cortisol, GH and insulin hormones. OBJECTIVE: To study the adjustments of plasma concentrations of the cortisol, GH and insulin hormones in resisted exercises of the same relative intensity with different muscle musses. METHODS: Ten volunteers, aged 20.3 ± 4.2 years, weight 74.1 ± 10.2 Kg, 177.2 ± 4.6 cm of stature and 23.8 ± 3.2 Kg/m2 of BMI, underwent a session of leg press (LP) and bench press (BP) with four sets of 10 repetitions at 70% 1 RM with three minutes apart. We collected blood samples to measure plasma concentrations of cortisol, GH and insulin at rest (Pre) and 0' (Rec. 0'), 30' (Rec. 30') and 90' (Rec. 90') of recovery. RESULTS: Plasma concentrations of cortisol decreased significantly at the end of the recovery in LP (2.20 ± 0.37 ng/dl to 1.33 ± 0.38 ng/dl) compared to pre. The GH and insulin concentrations significantly increased during recovery. GH was significantly higher in LP Rec. 0' (2.75 ± 3.29 ng/ml to 9.60 ± 5.32 ng/dl) than in pre. Insulin was significantly elevated in Rec. 30' in LP (14.70 ± 7.92 ulU/ml to 21.66 ± 8.61 ulU/ml) and BP (6.17 ± 2.99 ulU/ml to 19.70 ± 13.8 ulU/ml) for pre. The plasma insulin concentrations pre PL were significantly higher in the BP (14.70 ulU/ml and 6.17 ± 2.99 ulU/ml). CONCLUSION: Resisted exercise promoted different adjustments in hormone concentrations of cortisol, GH and insulin during the recovery period.

INTRODUCCIÓN: Muchos trabajos han estudiado el comportamiento hormonal en el ejercicio de resistencia, sin embargo, pocos se refieren el cortisol, GH y las hormonas insulina. OBJETIVO: Estudiar los ajustes de las concentraciones plasmáticas de las hormonas cortisol, GH e insulina en ejercicios resistidos, de igual intensidad, en relación con las masas musculares diferentes. MÉTODOS: Diez voluntarios, con 20,3 ± 4,2 años, 74,1 ± 10,2 kg de peso, 177,2 ± 4,6 cm de estatura y 23,8 ± 3,2 kg/m2 de IMC, realizaron una sesión de leg press (LP) y supino recto (SR) con cuatro series, de 10 repeticiones a 70% 1 RM, con tres minutos de intervalo. Se recolectaron muestras de sangre para dosificación de las concentraciones plasmáticas de cortisol, GH e insulina en reposo (Pré) y en 0' (Rec. 0'), 30' (Rec. 30') y 90' (Rec. 90') de recuperación. RESULTADOS: Las concentraciones plasmáticas de cortisol se redujeron significativamente al final de la recuperación en LP (2,20±0,37 ng/dl para 1,33±0,38ng/dl) en relación con la predosificación. Las concentraciones de GH e insulina aumentaron significativamente durante la recuperación. GH en LP fue significativamente mayor en Rec. 0' (2,75±3,29 ng/ml para 9,60±5,32 ng/dl) en comparación con la predosificación. La insulina se elevó significativamente en Rec. 30' en LP (14,70±7,92 ulU/ml para 21,66 ± 8,61 ulU/ml) y en SR (6,17 ± 2,99 ulU/ml para 19,70 ± 13,8 ulU/ml) en relación con la predosificación. Las concentraciones plasmáticas de insulina, predosificación, en LP fueron significativamente superiores a SR (14,70 ulU/ml y 6,17 ± 2,99 ulU/ml). CONCLUSIÓN: El ejercicio resistido causó diferentes ajustes en las concentraciones hormonales de cortisol, GH e insulina durante el período de recuperación.