Abstract The establishment of epigenetic marks during the reprogramming window is susceptible to environmental influences, and stimuli during this critical stage can cause altered DNA methylation in offspring. In a previous study, we found that low levels of sulphur and cobalt (low S/Co) in the diet offered to oocyte donors altered the DNA methylome of bovine embryos. However, due to the extensive epigenetic reprogramming that occurs during embryogenesis, we hypothesized that the different methylation regions (DMRs) identified in the blastocysts may not maintain in adulthood. Here, we aimed to characterize DMRs previously identified in embryos, in the blood and sperm of adult progenies of two groups of heifers (low S/Co and control). We used six bulls and characterized the DNA methylation levels of KDM2A, KDM5A, KMT2D, and DOT1L genes. Our results showed that all DMRs analysed in both groups and tissues were hypermethylated unlike that noticed in the embryonic methylome profiles. These results suggest that embryo DMRs were reprogrammed during the final stages of de novo methylation during embryogenesis or later in development. Therefore, due to the highly dynamic epigenetic state during early embryonic development, we suggest that is essential to validate the DMRs found in embryos in adult individuals.

RESUMO: Objetivou-se avaliar o efeito da substituição do milho pelo sorgo sobre o desempenho, rendimento de carcaça e composição dos cortes nobres em codornas para corte. Foram alojadas 1.200 codornas de corte, fêmeas de um dia de idade até os 42 dias, distribuídas em um delineamento inteiramente casualizado, composto de quatro tratamentos, com seis repetições cada, sendo que, em cada gaiola, foram alojadas 50 codornas. Os tratamentos consistiram de quatro níveis de substituição do milho pelo sorgo (0, 40, 60 e 100% de sorgo). Todas as aves foram pesadas para obtenção do ganho de peso. A conversão alimentar foi calculada pela relação entre o consumo de ração e o ganho de peso, considerando o peso das aves mortas. A mortalidade era registrada diariamente e calculada ao final de cada semana. Aos 42 dias, as aves foram abatidas e obtidos os rendimentos de carcaça, coxa+sobrecoxa e peito. Para os cortes de peito e coxa+sobrecoxa, foram realizadas análises de matéria mineral, extrato etéreo e proteína bruta. Não se observaram diferenças significativas nas variáveis de desempenho, rendimento e composição de cortes. Conclui-se que o sorgo grão moído pode substituir totalmente o milho em rações para codornas de corte, pois não compromete os índices de desempenho, rendimento e qualidade nutricional da carcaça.

ABSTRACT: The objective of this study was to evaluate the effect of replacing corn with sorghum in feed on performance, carcass yield, and composition of specialized meat cuts in quails. A total of 1200, 1-day-old female quails were raised up to 42 days of age. The completely randomized design consisted of four treatments with six replicates each and with 50 quails in each cage. Treatments consisted of four levels of sorghum replacement in the diet (0, 40, 60, and 100% sorghum). All birds were weighed to assess the weight gain. Feed conversion was calculated as the relationship between feed intake and weight gain. Mortality was reported daily and calculated at the end of each week. At 42 days, the birds were slaughtered and the carcass, thigh and drumstick, and breast yields were assessed. Mineral matter, ether extract, and crude protein analyses were performed using breast cuts and thigh + drumstick cuts. No significant differences were noted in cut performance, yield, or composition. Thus, it can be concluded that the ground grain sorghum can entirely replace corn in quail feed, as it does not negatively affect carcass performance, yield, and nutritional quality.