Objective: To evaluate macro and micronutrients intake of adolescents living with HIV/AIDS in use of antiretroviral therapy and compare it to the Dietary Reference Intakes. Methodology: Cross-sectional study conducted with adolescents of both genders with HIV/AIDS, assessing the dietary composition of macro and micronutrients, using the 24h dietary recall. Results: 39 adolescents, average age of 15 years, 51.3% males. The participants intake of total calories, total fiber (g/d), liposoluble vitamins (A, D, E, K), vitamin B5 (mg/d), vitamin B9 (mg/d), vitamin C (mg/d), calcium (mg/d), phosphorus (mg/d), potassium (mg/d), and magnesium (mg/d) was lower than recommended. The percentages of intake lower than recommended were 79.5% for calories, 82.1% for total fibers, 89.7% for vitamin A, 100% for vitamin D, 87.2% for vitamin E, 100% for vitamin K, 71.8% for vitamin B5, 82.1% for vitamin B9, 76.9% for vitamin C, 92.3% for calcium, 61.5% for phosphorus, 97.4% for potassium, and 76.9% for magnesium. The participants ingested more carbohydrates (g), proteins (g), vitamins B2 (mg/d), B3 (mg/d), B8 (mg/d) and sodium (g/d) than recommended, the percentages above the recommendations being 92.3% for carbohydrates, 64.1% for proteins and vitamin B2, 56.4% for vitamin B3, 82.1% for vitamin B8, and 59% for sodium. The remaining nutrients were within the amounts recommended by the DRIs. Conclusion: Food intake was inadequate as compared to the recommendations of the International Nutrition Guidelines.

Objetivo: identificar la ingestión alimentaria de macro y micronutrientes en adolescentes viviendo con VIH/sida que usan terapia anti-retroviral y compararlos a las Dietary Reference Intakes. Metodología: estudio transversal realizado con adolescentes de ambos sexos con VIH/sida, en el que se evaluó la composición dietética de macro y micronutrientes a través del recordatorio alimentario de 24h. Resultados: 39 adolescentes con una edad promedio de 15 años, un 51,3% del sexo masculino. Los participantes consumieron menos calorías totales en la dieta, fibra total (g/d), vitaminas liposolubles (A, D, E, K), vitamina B5 (mg/d), vitamina B9 (mg/d), vitamina C (mg/d), calcio (mg/d), fósforo (mg/d), potasio (mg/d) y magnesio (mg/d) que lo recomendado. Los porcentajes de ingestión por debajo de lo recomendado representaron el 79,5% para las calorías, el 82,1% para la fibra total, el 89,7% para la vitamina A, el 100% para la vitamina D, el 87,2% para la vitamina E, el 100% para la vitamina K, el 71,8% para la vitamina B5, el 82,1% para la vitamina B9, el 76,9% para la vitamina C, el 92,3% para el calcio, el 61,5% para el fósforo, el 97,4% para el potasio y el 76,9% para el magnesio. Los participantes consumían más carbohidratos (g), proteínas (g), vitaminas B2 (mg/d), B3 (mg/d), B8 (mg/d) y sodio (g/d) de lo recomendado, representando porcentajes por encima de la ingestión del 92,3% para los hidratos de carbono, del 64,1% para las proteínas y la vitamina B2, del 56,4% para la vitamina B3, del 82,1% para la vitamina B8 y del 59% para el sodio. Los demás nutrientes estaban dentro de lo recomendado por las DRIs. Conclusión: el consumo alimentario fue inadecuado en comparación con lo recomendado por las Directrices Internacionales de Nutrición.

INTRODUÇÃO: No contexto da cronicidade da AIDS, escassos estudos avaliaram variáveis de aptidão física em crianças e adolescentes; entretanto, com adultos têm-se registrado a importância da adesão aos exercícios físicos associados à TARV para melhorar essas variáveis. OBJETIVO: Identificar as características imunológicas, virológicas e as variáveis flexibilidade (FLEX) e força de resistência abdominal (FRA) de crianças e adolescentes portadores de HIV/AIDS em uso de TARV. MÉTODO: Estudo transversal realizado no Ambulatório de HIV/AIDS em Pediatria do Hospital de Clínicas de Porto Alegre. A amostra foi obtida consecutivamente por 63 pacientes (dez crianças e 53 adolescentes), de ambos os sexos, entre sete e 17 anos. A análise dos dados foi realizada no SPSS, versão 18.0, (p < 0,05). Para comparar as médias foi utilizado o teste t de Student pareado. RESULTADOS: O tempo médio de diagnóstico do HIV e da TARV atual foram 11 ± 3,42 anos e 40 ± 32,78 meses. A forma de transmissão prevalente foi a transmissão vertical (98,42%). A carga viral indetectável foi identificada em 73,1%. A contagem de T CD4+ e T CD8+, bem como sua relação, apresentaram as médias de 932,25 ± 445,53 células/ml, 1.018 ± 671,23 células/ml e 0,90 ± 0,41. Nas variáveis FLEX e FRA, independente do sexo, houve maior proporção de crianças e adolescentes classificadas abaixo dos pontos de corte. Observou-se diferença significativa entre a FRA e seus respectivos pontos de corte no período de diagnóstico do HIV (p = 0,032), T CD4+ (p = 0,008) e na carga viral (p = 0,030). Houve diferença significativa entre a FLEX e seus respectivos pontos de corte nas variáveis T CD4+/T CD8+ (p = 0,022) e na carga viral (p = 0,040). CONCLUSÃO: Os resultados demonstraram que existe uma estabilidade nas características imunológicas e virológicas, porém, níveis indesejados de aptidão física nas variáveis FLEX e FRA.

INTRODUCTION: In the context of chronicity of AIDS, few studies have evaluated variables of physical fitness in children and adolescents; however, with adults the importance of adherence to HAART associated with physical exercise to improve these variables has been reported. OBJECTIVE: To identify the immunological and virological characteristics and flexibility (FLEX) and abdominal endurance (AE) variables of children and HIV / AIDS adolescents using HAART. METHODS: This cross-sectional study took place at the HIV / AIDS Pediatric Clinic of the University Hospital ("Hospital de Clínicas") in the city of Porto Alegre. The sample was obtained consecutively by 63 patients (10 children and 53 adolescents) of both sexes, between ages 07 and 17 years. Data analysis was performed with SPSS, version 18.0 (p < 0.05). To compare the means, paired Student's t test was used. RESULTS: The mean diagnosis time of HIV and HAART was, respectively, 11 ± 3.42 years and 40 ± 32.78 months. The prevalent form of transmission was vertical transmission (98.42%). The undetectable viral load was identified in 73.1%. The count of CD4 + and CD8 + T cells as well as their relationship, presented averages of 932.25 ± 445.53 cells / ml, 1018 ± 671.23 cells / ml and 0.90 ± 0.41, respectively. Concerning the variables FLEX and AE, regardless of sex, there was a higher proportion of children and adolescents classified below the cutoffs points. Significant difference was observed between AE and their respective cutoff points in the HIV diagnosis (p = 0.032); CD4 + (p = 0.008) and viral load (p = 0.030). There were significant differences between FLEX and its respective cutoffs in variables CD4 + / CD8 + (p = 0.022) and in the viral load (p= 0.040). CONCLUSION: The results demonstrate that immunological and virological characteristics are stable; however, undesirable levels of fitness are observed in FLEX and AE variables.

Este trabalho teve como objetivo avaliar, em um intervalo de quatro anos, o desenvolvimento ósseo, dentário e cronológico de um grupo de crianças portadoras do HIV, comparando-o com um grupo controle. Foram realizadas radiografias panorâmica e de mão e punho, de uma amostra de 60 crianças, com idades variando entre 5 anos e 2 meses e 15 anos e 5 meses, dos sexos feminino e masculino, sendo: 30 crianças, voluntárias, portadoras de infecção pelo HIV, adquirida verticalmente, e 30 crianças voluntárias, que não apresentavam infecção pelo HIV ou qualquer outra doença sistêmica. Todas as radiografias foram padronizadas tecnicamente e analisadas segundo os critérios dos métodos de Nolla (idade dentária), Greulich e Pyle (idade óssea) e Eklöf e Ringertz (idade óssea). Os resultados foram submetidos ao teste estatístico t-Student em um nível de significância de 5%. Verificou-se, através da comparação da idade cronológica com as idades dentária e óssea, que houve diferença entre as crianças HIV+ e as não portadoras do vírus, tanto em 1999 como em 2003 (p < 0,05). Concluiu-se que as crianças HIV+ deste estudo sofreram um retardo no desenvolvimento ósseo. A idade dentária foi inferior à idade cronológica no grupo feminino HIV+, em 1999 e 2003, e masculino HIV+ em 1999.

The goal of this study was to evaluate skeletal, dental and chronological development in an HIV-positive group of children, as compared with a control group, during a four-year period. Panoramic radiographs and hand and wrist radiographs of 60 children were taken. The children, of both sexes, aged 5 years and 2 months to 15 years and 5 months, were selected as follows: 30 HIV-positive volunteers who had acquired the disease vertically, and 30 volunteers who did not present the HIV infection or any other systemic disease. All radiographs were technically standardized and analyzed according to criteria established by Nolla (dental age), Greulich and Pyle (bone age), and Eklöf and Ringertz (bone age). The results were submitted to Student's t-test at a 5% level of significance. Based on the comparison between the chronological age and the dental or the skeletal age, significant differences were observed between HIV-positive and HIV-negative children, both in 1999 and in 2003 (p < 0.05). Considering the results obtained with the methodology used, it was concluded that HIV-positive children of both sexes presented delayed bone development despite the administration of antiretroviral drugs, and that HIV-positive female children presented younger dental ages compared with their chronological ages in 1999 and in 2003; and HIV-positive males, in 1999.

Candida parapsilosis is an increasingly important bloodstream pathogen in neonatal intensive care units (NICU). We investigated a cluster of bloodstream infections in a NICU to determine whether nosocomial transmission occurred. During a 3-day period, 3 premature infants hospitalized in the same unit presented with sepsis caused by C. parapsilosis. Electrophoretic karyotype of the organisms was performed by using pulsed field gel electrophoresis in a countour-clamped homogeneous electric field system. The isolate from 1 newborn could not be typed, and the isolates from the remaining 2 infants had identical patterns. All 3 cases are described. We conclude that nosocomial transmission of C. parapsilosis occurred and that neonates under intensive care may represent a risk group for this pathogen.