Abstract: The article describes experiences in teaching the sensitive topic of the industrial extermination of European Jews during World War II (the Holocaust) in the BA German programme at Universiti Putra Malaysia. The topic was covered in a history course and supported by the use of international feature films. Based on a summary of Malaysia-Israel relations, the relevance of the topic in the teaching of German as a foreign language is highlighted, and the teaching objectives, pedagogical approach and results of a film project are presented. The article aims to answer the questions of how Malaysian students perceived history films on the Holocaust and whether it was possible to achieve a more differentiated view of the Holocaust, at least among some students. As part of a comprehensive history film project on the Second World War, three history films dealing with the persecution and extermination of European Jews were screened and analysed in class. The film 'The Grey Zone' (USA, 2001) was selected for this article and students' responses and attitudes to challenging questions, provided as qualitative data, are discussed. Three historical literacies – Content Knowledge, Historical Empathy/Perspective Recognition and Narrative Analysis ( Metzger 2007) – provided the theoretical framework for the history film project.

ABSTRACT Selection of a representative sampling area for accurate and reliable yield evaluation of wheat (Triticum aestivum L.) is important for optimizing straw retention in soils and its removal as biofuel feedstock. A field experiment was conducted at four wheat fields in the North China in 2020. Wheat straw and grain yields varied from 359.2-682.8 and 338.5-640.4 g m-2, respectively, and plant height varied from 51.3-59.7 cm across all plots in the four sites. Variation in either relative deviation (RD) or standard error (SE) of straw and grain yield and plant height estimates decreased with the increase in random sampling square (RSS) (one square = approximately 1 m²) and random sampling plant (RSP) numbers, respectively. Minimum RSS numbers of 3-10 and 1-10 m2 were needed to satisfy RD less than 5% in two-third of the plots for straw and grain yield estimates, respectively. This suggests that 10 m2 could be recommended as the minimum RSS number per plot. However, the incidence frequency of RD was 63.75% and 60.00% within the RD interval of 0%-5% for straw and grain yield estimates, respectively, from the RSS number of 10 m2, indicating that yields from RSSs in field trials are prone to large variations. Therefore, it is strongly recommended to design a large plot as possible and to harvest the whole plot for estimating yields. The threshold RSP number ranged from 14-18 to satisfy RD less than 1.5% and a minimum RSP of 20 plants (including mains and tillers) per plot could be recommended for wheat field experiments.

Abstract Background: Phospholipase C-like 1 (PLCL1), a protein that lacks catalytic activity, has similar structures to the PLC family. The aim of this research was to find the function and underlying mechanisms of PLCL1 in fibroblast-like synoviocyte (FLS) of rheumatoid arthritis (RA). Methods: In this study, we first analyzed the expression of PLCL1 in the synovial tissue of RA patients and K/BxN mice by immunohistochemical staining. Then silencing or overexpressing PLCL1 in FLS before stimulating by TNF-α. The levels of IL-6, IL-1β and CXCL8 in FLS and supernatants were detected by Western Blot (WB), Real-Time Quantitative PCR and Enzyme Linked Immunosorbent Assay. We used INF39 to specifically inhibit the activation of NLRP3 inflammasomes, and detected the expression of NLRP3, Cleaved Caspase-1, IL-6 and IL-1β in FLS by WB. Result: When PLCL1 was silenced, the level of IL-6, IL-1β and CXCL8 were down-regulated. When PLCL1 was overexpressed, the level of IL-6, IL-1β and CXCL8 were unregulated. The previous results demonstrated that the mechanism of PLCL1 regulating inflammation in FLS was related to NLRP3 inflammasomes. INF39 could counteract the release of inflammatory cytokines caused by overexpression of PLCL1. Conclusion: Result showed that the function of PLCL1 in RA FLS might be related to the NLRP3 inflammasomes. We finally confirmed our hypothesis with the NLRP3 inhibitor INF39. Our results suggested that PLCL1 might promote the inflammatory response of RA FLS by regulating the NLRP3 inflammasomes.

SUMMARY: The aim of this study was to explore promoting effect of external applying Panax Notoginseng Saponins (PNS) on fractures. For this analysis 18 New Zealand male rabbits were divided into control group, splintage group and PNS group. All rabbits were performed left radius fractures and natural healing, splintage healing and splintage coated with PNS healing. 2 rabbits in each group were sacrificed on day 14, day 28 and day 42 after surgery, separately. Atomic force microscope scanning and nanoindentation tests were performed on the callus sections. The particle size and roughness in PNS group was both less than that in splintage group. The elastic modulus of callus in PNS group was consistent with normal bone tissue started from day 28 after surgery, two weeks earlier than that in splintage group. PNS could significantly reduce fracture healing time and increase strength of callus.

RESUMEN: El objetivo de este estudio fue evaluar el efecto de la aplicación externa de Panax Notoginseng Saponins (PNS) en fracturas óseas. Se usaron 18 conejos machos de raza Nueva Zelanda divididos en grupos control, entablillado y PNS. Se realizaron fracturas del radio izquierdo y cicatrización natural en todos los animales, además de la cicatrización con entablillado y entablillado recubierto con PNS. Se sacrificaron, posterior a la cirugía, dos conejos de cada grupo los día 14, 28 y 42. Se realizaron pruebas de escaneo con microscopio de fuerza atómica y nanoindentación en las secciones de callos. El tamaño de la partícula y la rugosidad en el grupo de PNS fue menor que en el grupo entablillado. El módulo elástico del callo en el grupo de PNS fue consistente con el tejido óseo normal iniciado el día 28 después de la cirugía, dos semanas antes que en el grupo de entablillado. El PNS podría redu- cir significativamente el tiempo de curación de la fractura y aumentar la fuerza del callo.

ABSTRACT Objective We denote the four major factors related to the development of type 2 diabetes (T2D) as “diabetes factor” (DF); increased insulin resistance (IR); decreased glucose effectiveness (GE); and the first-and-second-phase of insulin secretion (FPIS, SPIS). The level of hemoglobin (Hb) was found to be related to IR and FPIS, but no-known studies focused on its role in relation to SPIS and GE. In this study, we aim to evaluate the relationships between Hb and all four DFs in the same individual. Subjects and methods We randomly enrolled 24,407 men and 24,889 women between 30 and 59 years old. IR, FPIS, SPIS and GE were measured according to equations published in our previous studies. To compare the slopes between Hb and the four DFs with different units, we converted their units to percent of change per unit of increased Hb. Results Age, HDL-cholesterol and GE were higher in women; BMI, blood pressure, LDL-cholesterol, TG, Hb, FPIS, SPIS and IR were higher in men. After they were converted into percentage, the closeness of their relationships to Hb, from the highest to the lowest, were GE, IR, FPIS and SPIS for women and IR, GE, FPIS and SPIS for men. GE was the only one negatively related to Hb. Conclusions Our data showed that IR, FPIS and SPIS were both positively and, GE negatively, related to the Hb in adult Chinese. For women, GE had the closest association with Hb; for men, it was IR. Both phases of insulin secretion had relatively weaker relationships than IR and GE.

Abstract Feature films in foreign-language education are a modern, efficient, motivating, stimulating and engaging medium for conveying and experiencing authentic language in real communication situations, for teaching target-oriented, country-specific cultural information as well as for developing intercultural awareness and communication skills. The paper examines the extent to which the use of feature film is actually implemented in L3 German classrooms in Malaysia. Based on two empirical studies conducted via standardized questionnaires and interviews with 43 teachers and 168 learners of German as a Foreign Language, the aim was to examine the importance of film material as a didactic instrument in German language programs and to analyze the motifs and didactic goals for implementing films. Students were interviewed about their media attitudes, their film viewing habits and genre preferences as well as about their perception of aspects of German culture in feature films. Although Malaysian learners of the German language show a great interest in Germany, its people, language, society and culture, teachers - for a variety of reasons but foremost caused by a lack of knowledge about the adequate implementation of feature films - have provided only few “magical moments” in L3 German classrooms thus far.

Zusammenfassung Spielfilme als dominante Ausdrucksform kultureller Identität sind ein modernes, motivierendes, emotional ansprechendes und effizientes (ergänzendes) Lehr- und Lernmedium im Sprachunterricht, dass die Lernenden in „realistisch wirkende Kommunikation“ und aktuelle Landeskunde einführt sowie interkulturelle kommunikative Kompetenz entwickelt. Das Interesse der Deutschlernenden an Spielfilmen ist groß und vor allem auf das Erleben authentischer Sprache in realen Situationen, auf den Alltag und die zwischenmenschlichen Beziehungen in deutschsprachigen Ländern ausgerichtet. In der Mehrzahl der außeruniversitären DaF-Programme in Malaysia ist das Medium Film trotz aller theoretischen Einsichten seitens der Lehrenden noch immer unterrepräsentiert und beschränkt sich zumeist auf reine Unterhaltung bzw. auf die Vermittlung von landeskundlichen Aspekten. Die Ursachen liegen in der fehlenden Aus- und Fortbildung im Bereich Filmarbeit im Fremdsprachenunterricht und der sich daraus ergebenden Unsicherheit der Lehrkräfte bei der zielgerichteten Implementierung filmischer Textsorten im Unterricht. Hinzu kommen ein stark prüfungszentrierter, traditionell grammatikorientierter Unterricht sowie Zeitmangel, aber auch das Desinteresse vieler Lehrender an der aufwändigen didaktisch-methodischen Vorentlastung und Nachbereitung einer Filmvorführung. Lediglich in den BA-German Programmen werden Filme in verschiedenen Kursen zur Vermittlung von Sprache, Landeskunde, Geschichte, Literatur, Kunst und zum fremd- und eigenkulturellen Vergleich eingesetzt. Am German-Malaysian-Institute dienen deutsche Spielfilme vor allem der interkulturellen Vorbereitung künftiger Studierender ingenieurswissenschaftlicher Programme an deutschen Fachhochschulen.

Um método novo, simples e sensível foi proposto para a determinação de mezlocilina. Baseia-se na amplificação da emissão de quimiluminescência (CL) gerada a partir da oxidação do luminol em meio alcalino pelo periodato de potássio. A otimização das variáveis experimentais e instrumentais que afetam o efeito da amplificação de CL foi realizada usando-se sistema de injeção em fluxo. Em condições ótimas, o método é eficiente para determinar mezlocilina no intervalo linear de 0,01 a 30 ×10-6 g mL-1 com limite de detecção (3σ) de 3,0×10-9 g mL-1 e desvio padrão relativo (RSD) de 1,0% para 1,0×10-6 g mL-1 de mezlocilina (n=11). O método tem sido aplicado com sucesso, para determinação de mezlocilina em preparações comerciais, amostras sintéticas e formulações biológicas fluidas.

A new, simple and sensitive method has been proposed for the determination of mezlocillin. It is based on the enhancement of the chemiluminescence (CL) emission generated from the oxidation of luminol in alkaline medium by postassium periodate. The optimization of the experimental and instrumental variables affecting the CL enhancement effect has been carried out using flow-injection system. In the optimum conditions, the method is efficient to determine mezlocillin in the linear range of 0.01-30×10-6 g mL-1 with a detection limit (3σ) of 3.0×10-9 g mL-1 and the relative standard deviation (RSD) is 1.0% for 1.0×10-6 g mL-1 mezlocillin (n=11). It has been successfully applied to the mezlocillin determination in commercial preparations, synthetic samples and biological fluids formulations.