ABSTRACT Epstein–Barr virus (EBV)-associated lymphoproliferative diseases (LPDs) cover a wide range of lymphocytes disorders spanning B, T and natural killer cells. The classification of EBV-associated LPD is evolving. We report a case of a 63-year-old male patient with EBV-associated T/B cell LPD diagnosed by pathological biopsy of lymph nodes, which is a new type of EBV-associated LPD that has not been reported previously. Because of its rarity, standard treatment has not been established. This patient showed rapid remission of the disease after combination chemotherapy with rituximab, which provided evidence supporting the use of rituximab and chemotherapy combination in EBV-associated T/B cell LPD treatment.

ABSTRACT Goose fatty liver is a delicious food product and the overfeeding will cause the abnormal physiology of the geese. The objective of this study was to investigate the effect of supplementation with hydrated sodium calcium aluminosilicate (HSCAS) on the fatty liver, ileal and cecal microbiota of Landes geese during overfeeding. Sixty 70-day-old Landes geese (body weight= 3.0 ± 0.05 kg) were randomly divided into three groups, two of which were overfed with whole corn supplemented with or without HSCAS for 20 days when the fatty liver reaches to the maximum size and the negative control group was ad libitum access to the corn basal diet. The intestinal contents of the ileum and cecum from three geese per group were used for high-throughput sequencing. As a result of this study, the HSCAS-treatment led to an increase in relative liver weight (p<0.05) of geese compared with the overfeeding control group. The richness and diversity of the bacterial communities decreased in the ileum and ceca after overfeeding. Overfeeding increased the relative abundance of Firmicutes, especially Lactobacillus, in ileal samples. HSCAS supplementation increased the relative abundance of Lactobacillus, and decreased the relative abundance of Actinobacillus in the ileum and the relative abundance of Erysipelotrichi, Bacteroides and Escherichia in the ceca. Bacterial richness indicators were also increased in samples from ileum and ceca after HSCAS supplementation. In conclusion, dietary HSCAS supplementation promoted liver performance in overfed Landes geese. HSCAS treatment had a beneficial effect on the intestinal microbiota composition in geese during the overfeeding.

ABSTRACT Ovoinhibitor (OIH) is the main proteinase inhibitor in the egg white. In the present study, real-time quantitative PCR and Western-Blot were used to analyze different expression pattern of OIH in ovaries as a candidate gene of reproductive traits in Jingjiang ducks (JJ ducks) and Shaoxing ducks (SX ducks) during three laying stages. To study the polymorphism of the OIH gene in those two duck populations, we designed five pairs of primers to detect SNPs of exon 3-5, 5-6, 14-16 and intron 7, 9 of the OIH gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA pool sequencing methods. The results showed that OIH expression increased during the laying stage in the ovaries of both duck breeds. The relative expression levels of OIH in the egg at hatch and 180days of age were lower in JJ ducks than in SX ducks, but higher in JJ ducks than SX ducks at 500 days of age. Only exon 5-6 locus had a novel SNP. One variation (389G>A) was detected in the two tested duck populations and it was associated with some laying traits, such as body weight of hatch, age at first egg, weight at first egg, egg number at 72weeks of age. The AG genotype was associated with inferior body weight of hatch and superior weight at first egg, age at first egg and egg number at 72weeks of age. Therefore, these results suggest that OIH may be a strong candidate gene related to some laying traits in ducks.

ABSTRACT Graft-versus-host disease (GVHD) is a lethal complication of allogeneic haematopoietic stem cell transplantation which limits its application. Psoralen was utilized in the treatment of GVHD as a second-line method, which is also known as extracorporeal photochemotherapy (ECP). In the process of ECP, mononuclear cells must be isolated from the body in advance. Then a photosensitizer, 8-methoxypsoralen (8-MOP, 200 μg/L for the final concentration), would be added to the cell suspension before its exposure to ultraviolet A [UVA; 365 nm, 2J/cm2]. The disposed lymphocytes re-infused into the body account for 5% to 15% of the lymphocytes in the body. The process of ECP is complicated, expensive and very labour intensive, which limits its popularity. We hypothesized that psoralen should be activated by UVA, and should be kept in activation for a relatively long time before it gets in contact with mononuclear cells. This kind of psoralen is called previously activated psoralen (PAP), which may have the same effects on GVHD as ECP, but would be much easier and economical to work with.

RESUMEN La enfermedad de injerto-contra-huésped (EICH, o GVHD por las siglas en inglés) es una complicación letal del trasplante alogénico de células madre hematopoyéticas que limita su aplicación. El psoraleno se utilizó en el tratamiento de EICH como método de segunda línea, también conocido como fotoquimioterapia extracorpórea (F EC). En el proceso de FEC, las células mononucleares tienen que ser aisladas del cuerpo por adelantado. Entonces un fotosensibilizador, 8-methoxypsoraleno (8-MOP, 200 μg/L para la concentración final), se añadirá a la suspensión de células antes de su exposición a radiación ultravioleta A [UVA; 365 nm, 2J/cm2]. Los linfocitos eliminados re-infundidos en el cuerpo representan del 5% al 15% de los linfocitos en el cuerpo. El proceso de FEC es complicado, costoso, y requiere mucho trabajo, lo cual limita su popularidad. Partimos de la hipótesis de que el psoraleno debe ser activado por rayos UVA y debe mantenerse en activación por un tiempo relativamente largo, antes de que se ponga en contacto con las células mononucleares. Este tipo de psoraleno se denomina psoraleno activado previamente (PAP), el cual puede tener los mismos efectos sobre la EICH que la FEC, pero de manera mucho más fácil y económica.