ABSTRACT Background: Alpha 1-antitrypsin deficiency (AATD) is a hereditary codominant autosomal disease. This liver disease ranges from asymptomatic cases to terminal illness, which makes early recognition and diagnosis challenging. It is the main cause of pediatric liver transplantation after biliary atresia. Objective: To describe the clinical characteristics, as well as those of histologic and laboratory tests, phenotypic and/or genetic evaluation and evolution of a cohort of pediatric patients with AATD. Methods: This is a retrospective observational study of 39 patients with confirmed or probable AATD (without phenotyping or genotyping, but with suggestive clinical features, low serum alpha 1-antitrypsin (AAT) level and liver biopsy with PAS granules, resistant diastasis). Clinical, laboratory and histological variables, presence of portal hypertension (PH) and survival with native liver have been analyzed. Results: A total of 66.7% of 39 patients were male (26/39). The initial manifestation was cholestatic jaundice in 79.5% (31/39). Liver transplantation was performed in 28.2% (11/39) of patients. Diagnosis occurred at an average of 3.1 years old and liver transplantation at 4.1 years of age. 89.2% (25/28) of the patients with confirmed AATD were PI*ZZ or ZZ. The average AAT value on admission for PI*ZZ or ZZ patients was 41.6 mg/dL. All transplanted patients with phenotyping or genotyping were PI*ZZ (or ZZ). Those who were jaundiced on admission were earlier referred to the specialized service and had higher levels of GGT and platelets on admission. There was no significant difference in the survival curve when comparing cholestatic jaundiced to non-cholestatic jaundiced patients on admission. Comparing patients who did or did not progress to PH, higher levels of AST and APRI score at diagnosis (P=0.011 and P=0.026, respectively) were observed and in the survival curves patients with PH showed impairment, with 20.2% survival with native liver in 15 years. Conclusion: Jaundice is an important clinical sign that motivates referral to a specialist, but it does not seem to compromise survival with native liver. Patients progressing to PH had higher AST, APRi score on admission and significantly impaired survival with native liver. It is important to pay attention to these signs in the follow-up of patients with AATD.

RESUMO Contexto: Deficiência de alfa 1-antitripsina (DAAT) é uma doença hereditária, de caráter autossômico codominante. A apresentação da doença hepática varia desde casos assintomáticos até doença terminal, o que dificulta reconhecimento e diagnóstico precoces. É a principal causa de transplante hepático pediátrico após atresia de vias biliares. Objetivo: Descrever as características clínicas, de exames laboratoriais, histológicos, avaliação fenotípica e/ou genética e sobrevida de uma coorte de pacientes pediátricos com DAAT. Métodos: Estudo observacional retrospectivo de 39 pacientes com diagnóstico de DAAT confirmada ou provável (sem fenotipagem ou genotipagem, mas com clínica sugestiva, baixo nível sérico de alfa 1-antitripsina (A1AT) e biópsia hepática com grânulos PAS, diástase resistentes). Variáveis clínicas, laboratoriais, histológicas, presença de hipertensão portal (HP) e sobrevida com fígado nativo foram analisadas. Resultados: Dos 39 pacientes, 66,7% eram do sexo masculino (26/39). A manifestação inicial foi icterícia colestática em 79,5% (31/39). Em 28,2% (11/39) houve necessidade de transplante hepático. O diagnóstico ocorreu com uma idade média de 3,1 anos e, o transplante hepático, 4,1 anos. Dos pacientes com DAAT confirmada, 89,2% (25/28) eram PI*ZZ ou ZZ. O valor médio de A1AT na admissão de pacientes PI*ZZ ou ZZ foi 41,6 mg/dL. Todos os transplantados com fenotipagem ou genotipagem eram PI*ZZ (ou ZZ). Os ictéricos à admissão foram referenciados mais cedo ao serviço especializado e apresentaram níveis mais elevados de GGT e plaquetas à admissão. Não houve diferença significativa na curva de sobrevida ao compararmos icterícia colestática ou não à admissão. Ao comparar os pacientes que progrediram ou não para HP, observou-se níveis mais elevados de AST e APRI escore ao diagnóstico (P=0,011 e P=0,026, respectivamente) e, nas curvas de sobrevida, pacientes com HP apresentaram comprometimento, com 20,2% de sobrevida com fígado nativo em 15 anos. Conclusão: Icterícia é um sinal clínico importante que motiva o encaminhamento ao especialista, mas parece não comprometer a sobrevida com fígado nativo. Pacientes com evolução para HP tiveram AST e escore APRi mais elevados à admissão e comprometimento significativo da sobrevida com fígado nativo. Importante atentar a esses sinais no seguimento de pacientes com DAAT.

Abstract Objective The aim of this study was to analyze the effects of fluid overload related to mechanical ventilation, renal replacement therapy, and evolution to discharge or death in critically ill children. Methods A retrospective study in a Pediatric Intensive Care Unit for two years. Patients who required invasive ventilatory support and vasopressor and/or inotropic medications were considered critically ill. Results 70 patients were included. The mean age was 6.8 ± 6 years. There was a tolerable increase in fluid overload during hospitalization, with a median of 2.45% on the first day, 5.10% on the third day, and 8.39% on the tenth day. The median fluid overload on the third day among those patients in pressure support ventilation mode was 4.80% while the median of those who remained on controlled ventilation was 8.45% (p = 0.039). Statistical significance was observed in the correlations between fluid overload measurements on the first, third, and tenth days of hospitalization and the beginning of renal replacement therapy (p = 0.049) and between renal replacement therapy and death (p = 0.01). The median fluid overload was 7.50% in patients who died versus 4.90% in those who did not die on the third day of hospitalization (p = 0.064). There was no statistically significant association between death and the variables sex or age. Conclusions The fluid overload on the third day of hospitalization proved to be a determinant for the clinical outcomes of weaning from mechanical ventilation, initiation of renal replacement therapy, discharge from the intensive care unit, or death among these children.

ABSTRACT Background: Most data on the natural history of portal hypertension come from studies in adults. The morbidity rate of upper gastrointestinal bleeding (UGIB) in children with portal hypertension has not been systematically characterized. Objective: To describe the morbidity and mortality of UGIB in pediatric patients with portal hypertension and identify predictive factors for the occurrence of its main complications. Methods: This retrospective study included pediatric patients with cirrhotic portal hypertension or with extrahepatic portal vein obstruction (EHPVO). Mortality and UGIB complications within a period of up to 6 weeks of the bleeding were investigated. To determine the predictive factors of morbidity, a multivariate analysis was performed using logistic regression; all results were considered significant at P<0.05. Results: A total of 86 patients (51.2% with EHPVO and 48.8% with cirrhosis) had 174 bleeding events. Ascites was the most common complication (43.1% of all cases), being more prevalent in patients with cirrhosis (P<0.001). Cirrhosis was a predictor of the occurrence of any morbidity (OR 20.3). The need for blood transfusion was predictor of at least one complication (OR 5.8), ascites (OR 7.2) and infections (OR 3.8) in the general group and at least one complication (OR 11.3) and ascites (OR 5.8) in cirrhotic patients. The need for expansion was a predictor of any morbidity (OR 4.6) and infections (OR 3.9) in the general group, in addition to being predictor of infection in cirrhotic patients (OR 5.4). There were no deaths from UGIB in the six weeks post-bleeding. Conclusion: The study showed the relevance of morbidity after UGIB in pediatric patients with portal hypertension, especially in those with cirrhosis. The patients with hemodynamic instability requiring blood transfusion or expansion on admission are at increased risk of complications related to upper gastrointestinal bleeding and should be closely monitored.

RESUMO Contexto: A maioria dos dados sobre a história natural da hipertensão porta provém de estudos em adultos. A morbidade associada à hemorragia digestiva alta (HDA) em crianças com hipertensão porta ainda não foi sistematicamente estudada. Objetivo: Descrever a morbimortalidade da HDA em pacientes pediátricos com hipertensão porta e identificar fatores preditivos para a ocorrência de suas principais complicações. Métodos: Este estudo retrospectivo incluiu pacientes pediátricos com hipertensão porta cirrótica ou com obstrução extra-hepática da veia porta (OEHVP). A mortalidade e as complicações da HDA foram estudadas até seis semanas após o sangramento. Para determinar os fatores preditivos de morbidade, foi realizada análise multivariada por meio de regressão logística; todos os resultados foram considerados significativos com P<0,05. Resultados: Oitenta e seis pacientes (51,2% com OEHVP e 48,8% com cirrose) tiveram 174 eventos hemorrágicos. A ascite foi a complicação mais comum (43,1% de todos os casos), sendo mais prevalente em pacientes com cirrose (P<0,001). A cirrose foi preditor da ocorrência de pelo menos uma complicação (OR 20,3). A necessidade de transfusão sanguínea foi preditora de pelo menos uma complicação (OR 5,8), ascite (OR 7,2) e infecções (OR 3,8) no grupo geral e pelo menos uma complicação (OR 11,3) e ascite (OR 5,8) nos cirróticos. A necessidade de expansão foi preditor de qualquer morbidade (OR 4,6) e infecções (OR 3,9) no grupo geral, além de ser preditor de infecção em cirróticos (OR 5,4). Não houve óbitos por HDA nas 6 semanas pós-sangramento. Conclusão: O estudo mostrou a relevância da morbidade após HDA em pacientes pediátricos com hipertensão porta, principalmente naqueles com cirrose. Os pacientes com instabilidade hemodinâmica que necessitam de transfusão de sangue ou expansão na admissão têm risco aumentado de complicações relacionadas à hemorragia digestiva alta e devem ser monitorados de perto.

Abstract Introduction The immune reconstitution (IR) after the allogenic hematopoietic stem cell transplantation (allo-HSCT) is a progressive process intrinsically correlated to the therapeutic success. It is essential to understand the interfering factors in IR to prevent the HSCT-related mortality. Methods We retrospectively evaluated the clinical outcomes, absolute lymphocyte counts (ALCs) and lymphocyte subtypes at different time-points of 111 pediatric patients with allogeneic HSCT for malignant and non-malignant diseases from 2013 to 2018. Results The ALCs gradually increased on D+30, D+100, and D+180 (medians 634/μL, 1022/μL and 1541/μL, respectively). On D+100, the CD3+CD8+ achieved the highest recovery rate (68%), followed by the CD16+CD56+ (47%), CD3+CD4+ (39%) and CD19+ (8%). The adequate ALC recovery was associated with age < 8 years, bone marrow grafts, myeloablative conditioning, non-use of serotherapy and non-haploidentical donors. The ALC and CD3+CD8+ on D+100 counts were higher in patients with the cytomegalovirus infection. The CD3+CD4+ recovery was associated with an age < 8 years, a non-malignant disease and a lower incidence of acute graft-versus-host disease ≥ grade 2. Furthermore, the ALC recovery on D+100 resulted in a higher overall survival, regardless of the disease type (HR 3.65, 1.05 - 12.71, p= 0.04). Conclusion Several factors influenced the IR after the allo-HSCT. The ALC ≥ 500/μL on D+100 was a simple IR predictor of survival, easily available to resource-limited centers.

ABSTRACT In the Prata watershed there are units corresponding to the Pleistocene Terraces and the Fluvium-Lagoon Sediments. This watershed is inserted almost entirely in the area of plateaus (Tabuleiros) with altitudes of 10 to 100 meters, and in areas with altitudes of 2 to 10 meters in the area of floodplains and fluvio-lagoon terraces. The present work aimed to characterize and classify soils in the Brazilian Soil Classification System - SiBCS and correlate them with the IUSS Working Group WRB-FAO (in parentheses), besides evidence some soil-landscape relations, that occur in Pleistocene terraces and fluvium-lagoon sediments in the Prata watershed, located in the ecological reserve of Dois Irmãos State Park in Recife – PE, Brazil. Soil samples were used to perform physical, chemical and mineralogical analysis in laboratory. Watershed delimitation, Digital Elevation Model (DEM), slope map, soil map and slope x soil map were obtained and studied. The soils are quite acidic and have a very low natural fertility, with the Latossolos (Ferralsols) predominating in the landscape. The silt and clay fractions of the studied Argissolos (Acrisols) and Latossolos (Ferralsols) presented quartz and kaolinite as the main component. In the profile of Neossolo Quartzarênico (Arenosols) only quartz was identified as a component of the silt and clay fractions. The kaolinitic mineralogy of the clay fraction is consistent with the environment of its formation, that is, hot and humid. There is no relationship between the colors of soils and their exposure to the sun on the slopes where they occur. The occurrence of a sandy and whitish horizon between the litter and the A horizon, is not foreseen in the norms and criteria adopted in the Brazilian Soil Classification System - SiBCS.

ABSTRACT Seed quality plays an important role in the production of soybean. The objective of this study was to quantify the effects of producing region on seed chemical composition (oil, protein, and fatty acid content) and physiological quality of soybean. Twenty-eight soybean cultivars were evaluated in 2017/18 and 2018/19 crop seasons, and field trials were performed in different environments classified as high (846–963 m above sea level [asl]) or low altitude (336–480 m asl). The evaluated traits were percentage germination, accelerated aging, germination test, emergence speed index, and seed chemical composition (oil and protein contents, fatty acid profile). A significant effect of cultivar × environment interactions on all evaluated traits was observed. High-altitude environments produce soybeans with a greater protein content, and low altitudes yielded seeds with elevated oil content. Higher protein-content seeds have greater physiological potential, and seeds with higher oil content are negatively associated with physiological potential. High-altitude environments maximized the physiological quality of seeds. Linking genetics to target populations of environments ensures seed quality and benefits the entire soybean production chain.

RESUMO Os beta-lactâmicos constituem a causa mais frequente de reações de hipersensibilidade a fármacos mediadas por mecanismo imunológico específico. As reações imediatas ocorrem em 1 até 6 horas após a administração do beta-lactâmico, sendo geralmente IgE-mediadas. Elas se traduzem clinicamente por urticária, angioedema e anafilaxia. As reações não imediatas ou tardias ocorrem após 1 hora da administração. São as reações mais comuns, sendo geralmente mediadas por células T. O tipo mais frequente é o exantema maculopapular ou morbiliforme. A maioria dos indivíduos que refere alergia aos beta-lactâmicos pode tolerar esse grupo de antibióticos. No diagnóstico, uma história clínica detalhada é fundamental para verificar se a reação foi do tipo imediato ou não imediato. A partir daí, podem ser realizados testes in vivo e/ou in vitro para investigação. O teste de provocação é considerado o método padrão-ouro no diagnóstico de hipersensibilidade aos beta-lactâmicos. A primeira conduta diante da suspeita de uma reação ao beta-lactâmico é suspender a exposição ao medicamento, e o único tratamento específico é a dessensibilização, que possui indicações bem precisas. O diagnóstico equivocado de alergia à penicilina afeta o sistema de saúde, pois o rótulo de “alergia à penicilina” está associado a aumento da resistência bacteriana, maior índice de falha terapêutica, hospitalizações prolongadas, readmissões e aumento dos custos. Assim, torna-se fundamental elaborar estratégias com o objetivo de auxiliar na prescrição de antibióticos em pacientes com rótulo de “alergia aos beta-lactâmicos” nos hospitais e melhorar a educação dos pacientes e seus responsáveis, além de médicos não especialistas.

ABSTRACT Betalactams are the most frequent cause of hypersensitivity reactions to drugs mediated by a specific immune mechanism. Immediate reactions occur within 1 to 6 hours after betalactam administration, and are generally IgE-mediated. They clinically translate into urticaria, angioedema and anaphylaxis. Non-immediate or delayed reactions occur after 1 hour of administration. These are the most common reactions and are usually mediated by T cells. The most frequent type is the maculopapular or morbilliform exanthematous eruption. Most individuals who report allergies to penicillin and betalactams can tolerate this group of antibiotics. To make diagnosis, a detailed medical history is essential to verify whether it was an immediate or non-immediate reaction. Thereafter, in vivo and/or in vitro tests for investigation may be performed. The challenging test is considered the gold standard method for diagnosis of betalactam hypersensitivity. The first approach when suspecting a reaction to betalactam is to discontinue exposure to the drug, and the only specific treatment is desensitization, which has very precise indications. The misdiagnosis of penicillin allergy affects the health system, since the “penicillin allergy” label is associated with increased bacterial resistance, higher rate of therapeutic failure, prolonged hospitalizations, readmissions, and increased costs. Thus, it is essential to develop strategies to assist the prescription of antibiotics in patients identified with a label of “betalactam allergy” at hospitals, and to enhance education of patients and their caregivers, as well as of non-specialist physicians.

Resumo Justificativa: A administração inadvertida peridural de drogas está associada à morbidade e mortalidade. Várias drogas foram administradas acidentalmente pelo cateter peridural e a maior parte do que sabemos se baseia em relatos de caso. Relato de caso: Uma gestante de 33 anos chegou em trabalho de parto. Foi solicitada colocação de cateter peridural para analgesia de parto e a dose inicial foi administrada. Cinco minutos depois, 1 g de ampicilina foi dado através do cateter inadvertidamente, sem alterações hemodinâmicas ou neurológicas. A administração de ropivacaína foi repetida, sempre com alívio dos sintomas até o parto. Na alta hospitalar, a paciente continuava sem alterações neurológicas ou hemodinâmicas. Conclusões: A maioria dos erros é por troca de seringa ou drogas, ou administração de rota inadvertida. A administração errônea no espaço peridural pode apresentar efeitos imediatos e tardios e não há tratamento definitivo ou efetivo. Existem várias medidas preventivas para reduzir complicações potenciais; alguns escolhem observação cuidadosa, outros a administração de outras drogas para tentar a diluição.

Abstract Background: Inadvertent epidural drug administration is associated with morbidity and mortality. Several drugs have been administered accidentally through the epidural catheter and most of our knowledge is based on case reports. Case report: A 33 year-old woman presented for delivery. Placement of epidural catheter was requested for labor analgesia and priming dose was administered. Five minutes later, ampicillin 1 g was given through the catheter inadvertently without hemodynamic or neurological changes. Ropivacaine administration was repeated, always with symptomatic relief until delivery. At hospital discharge, she remained without neurological or hemodynamic alterations. Conclusions: The majority of errors are due to syringe and drug exchanges and inadvertent route administration. Erroneous administration into the epidural space can have immediate and late effects and there is no definitive and effective treatment. There are several preventive measures to reduce the potential complications; some opt for watchful waiting, others opt for administering other drugs as a dilution attempted.

Resumo Justificativa e objetivos: A ventilação monopulmonar e a intubação seletiva em recém-nascidos podem ser um desafio devido às especificidades fisiológicas intrínsecas e ao material disponível. O aparelho de ultrassom tem sido cada vez mais usado em muitas situações no campo da anestesia, incluindo a confirmação da posição do tubo endotraqueal. Relato de caso: Apresentamos o relato do caso de um recém-nascido proposto para lobectomia pulmonar por toracoscopia em que a exclusão pulmonar foi confirmada por ultrassom. Conclusão: O ultrassom é um método rápido, mais sensível e específico do que a ausculta para avaliar a intubação traqueal e a exclusão pulmonar.

Abstract Background and objectives: One-lung ventilation and selective intubation in neonates can be challenging due to intrinsic physiological specificities and material available. Ultrasound (US) is being increasingly used in many extents of anaesthesiology including confirmation of endotracheal tube position. Case report: We present a case report of a neonate proposed for pulmonary lobectomy by thoracoscopy in which lung exclusion was confirmed by ultrasound. Conclusion: US is a rapid, more sensitive and specific method than auscultation to evaluate tracheal intubation and lung exclusion.

ABSTRACT: The article aimed to describe the men’s perception about prostate cancer and related prevention factors. This is a qualitative community trial type study. Three meetings were held in the form of a focus group, composed of 60 men aged over 40 years old, addressing the topic of prostate cancer. In order to analyze the qualitative data, thematic content analysis was performed and the data were organized in the software - Atlas Ti. The results were referenced in the objectives of this study and emphasized into categories. The data showed that there is still a physical and social barrier to overcome with male stigmas, and there is a lack of knowledge about the prevention of this cancer. The increase in the availability of diagnostic tests, agility in care, and differentiated schedules for workers are some of the efficient strategies to attract this population to the prevention of prostate cancer.

RESUMO: O artigoobjetivoudescrever a percepção dos homens sobre o câncer de próstata e os fatores de prevenção relacionados. Trata-se de um estudo qualitativo do tipo ensaio comunitário. Foram realizadas três reuniões em forma de grupo focal, composto por 60 homens com faixa etária superior a 40 anos, abordando o tema do câncer de próstata. Para a análise dos dados qualitativos foi realizada a análise de conteúdo temática e os dados foram organizados no software - Atlas Ti. Osresultados foram referenciados nos objetivos deste estudo e enfatizados em categorias. Os dados evidenciaram que ainda há uma barreira física e social a ser ultrapassada diante dos estigmas masculinos, e existe uma carência de conhecimento sobre a prevenção deste câncer. O aumento da oferta de exames diagnósticos, agilidade no atendimento, horários diferenciados para os trabalhadores são algumas das estratégias eficientes para atrair esta população para a prevenção do câncer de próstata.

RESUMEN: El artículoobjetivódescribir la percepción de los hombres sobre el cáncer de próstata y los factores de prevención relacionados. Se trata de un estudio cualitativo del tipo de ensayo comunitario. Se realizaron tres reuniones en forma de grupo focal, compuesto por 60 hombres con rango de edad superior a 40 años, abordando el tema del cáncer de próstata. Para el análisis de los datos cualitativos se realizó el análisis de contenido temático y los datos fueron organizados en el software - Atlas Ti. Losresultadosfueron referenciados en los objetivos de este estudio y enfatizados en categorías. Los datos evidenciaron que todavía hay una barrera física y social a ser superada ante los estigmas masculinos, y existe una carencia de conocimiento sobre la prevención de este cáncer. El aumento de la oferta de exámenes diagnósticos, agilidad en la atención, horarios diferenciados para los trabajadores son algunas de las estrategias eficientes para atraer a esta población para la prevención del cáncer de próstata.

A new study about corrosion behavior of CoCrMo alloy used as implant material exposed to the physiological serum and Hank's solution in different temperatures, electrolyte compositions and immersion time were simulated, using open circuit potential (OCP), potentiodynamic polarization curves (PPC) and electrochemical impedance spectroscopy (EIS.) From the results, it can be concluded that CoCrMo biomaterial is influenced by electrolyte composition and temperature, and the passive films were more protective when formed in presence of physiological serum. Comparing the Ecorr for both solutions, it is possible to verify that the Hank's solution presented values more negative for the two studied temperatures (25 and 37 °C), indicating a greater susceptibility to the corrosion process. Regarding the results of potentiodynamic polarization, the ipass, decreased as function of time, indicating a more protective passive film at 25 °C. In regards to the physiological serum, the highest ipass values at 37 ºC evidenced the formation of a less stable passive film. The different behavior of ipass in Hank's solution may be related to the competitiveness between a possible film of D-glucose and oxides of the alloy metals. EIS studies suggest that the film formed in presence of physiological serum is nobler than that with Hank's solution.

Resumo Espécies do gênero Hepatozoon são os hemoparasitas intracelulares mais comumente encontrados em répteis. Hepatozoon caimani, cujos vetores são mosquitos do gênero Culex sp., têm sido detectados em uma alta prevalência entre jacarés no Brasil, por meio da análise de esfregaços sanguíneos. O presente estudo objetivou detectar e caracterizar parasitas do gênero Hepatozoon spp. em 33 jacarés (24 de vida-livre e 9 de cativeiro; 28 machos e 5 fêmeas) (Caiman crocodilus yacare) amostrados em Poconé, região norte do Pantanal, estado do Mato Grosso, Brasil, por meio da análise de esfregaços sanguíneos e técnicas moleculares. Gametócitos de Hepatozoon spp. foram encontrados em 70,8% (17/24) e em 88,8% (8/9) dos esfregaços sanguíneos de jacarés de via-livre e cativeiro, respectivamente. 18S rRNA DNA de Hepatozoon spp. foi detectado em 79,2% (19/24) e 88,8% (8/9) das amostras de sangue de jacarés de vida-livre e cativeiro, respectivamente. A análise comparativa de eritrócitos parasitados e não parasitados mostrou diferença significativa (P<0,05) em todas as variáveis lineares e de área analisadas. A análise filogenética baseada em sequências de DNA do 18S rRNA agrupou as sequências de Hepatozoon spp. detectadas no presente estudo juntamente com aquelas de H. caimani, recentemente detectadas em jacarés do Pantanal do Mato Grosso do Sul.

Abstract Hepatozoon species are the most common intracellular hemoparasite found in reptiles. Hepatozoon caimani, whose vectors are Culex mosquitoes, has been detected in a high prevalence among caimans in Brazil by blood smears examinations. The present work aimed to detect and characterize the Hepatozoon spp. found in 33 caimans (24 free-ranging and 9 captive; 28 males and 5 females) (Caiman crocodilus yacare) sampled at Poconé, North Pantanal, state of Mato Grosso, Brazil, using blood smears examinations and molecular techniques. Hepatozoon spp.-gametocytes were found in 70.8% (17/24) and 88.8% (8/9) of blood smears from free-ranging and captive caimans, respectively. Hepatozoon spp. 18S rRNA DNA was found in 79.2% (19/24) and 88.8% (8/9) of free-ranging and captive caimans, respectively. Comparative analysis of parasitized and non-parasitized erythrocytes showed that all analyzed features were significantly different (P<0.05) for both linear and area dimensions. Phylogenetic analysis based on 18S rRNA sequences grouped the Hepatozoon spp. sequences detected in the present study together with H. caimani, recently detected in caimans in southern Pantanal.

Resumo Objetivamos analisar a distribuição geográfica, a estrutura de atendimento e os recursos humanos das unidades do Sistema Único de Saúde (SUS) que prestam serviços em saúde sexual e reprodutiva (SSR) à população adolescente, na segunda maior cidade do Brasil. Realizamos estudo transversal com mapeamento geográfico e coleta de dados por meio de questionário aplicado aos coordenadores, em todas as 147 unidades ambulatoriais do Rio de Janeiro, dispostas ao atendimento em SSR. Verificamos que todas as Regiões Administrativas (RA) desse município dispõem de unidades de atendimento para adolescentes, entretanto, em mais de 90% delas, eles são atendidos juntamente com a população adulta, sem turnos ou salas específicas. Em mais de 10% dos serviços, a assistência só é dada na presença do responsável. Em casos de violência sexual a proporção mostrou-se ainda maior, chegando a atingir 34% dos casos. Em apenas 12,9% das unidades são realizadas atividades educativas direcionadas a adolescentes e constatou-se que menos de 1/3 dos médicos estava capacitado para lidar com a saúde do adolescente. Em conclusão, apesar da larga distribuição geográfica das unidades de saúde, a estrutura do atendimento e os recursos humanos não atendem a contento as especificidades do público adolescente.

Abstract We aimed to analyze the geographic distribution, the structure of healthcare services and the human resources of all units of the Sistema Único de Saúde (SUS - the Unified Health System) that provide sexual and reproductive health (SRH) services to the adolescent population in the second largest city in Brazil. We conducted a cross-sectional study with geographical mapping and data collection through a questionnaire applied in person with coordinators of the units or their representatives in 147 outpatient clinics in Rio de Janeiro that have SSR services. We found that in over 90% of the units, adolescents are treated together with the adult population, without particular shifts or rooms for this age group. In more than 10% of services, treatment is only provided with the presence of the guardian. In cases of sexual violence, this proportion is 34%. Specific educational activities for this age group are only carried out in 12.9% of units and less than one third of doctors had received some kind of training to deal with adolescent health. In conclusion, despite the wide geographic distribution of health facilities, the structure of care and the human resources do not meet the specific needs of adolescents.

Resumo Objetivamos analisar a distribuição geográfica, a estrutura de atendimento e os recursos humanos das unidades do Sistema Único de Saúde (SUS) que prestam serviços em saúde sexual e reprodutiva (SSR) à população adolescente, na segunda maior cidade do Brasil. Realizamos estudo transversal com mapeamento geográfico e coleta de dados por meio de questionário aplicado aos coordenadores, em todas as 147 unidades ambulatoriais do Rio de Janeiro, dispostas ao atendimento em SSR. Verificamos que todas as Regiões Administrativas (RA) desse município dispõem de unidades de atendimento para adolescentes, entretanto, em mais de 90% delas, eles são atendidos juntamente com a população adulta, sem turnos ou salas específicas. Em mais de 10% dos serviços, a assistência só é dada na presença do responsável. Em casos de violência sexual a proporção mostrou-se ainda maior, chegando a atingir 34% dos casos. Em apenas 12,9% das unidades são realizadas atividades educativas direcionadas a adolescentes e constatou-se que menos de 1/3 dos médicos estava capacitado para lidar com a saúde do adolescente. Em conclusão, apesar da larga distribuição geográfica das unidades de saúde, a estrutura do atendimento e os recursos humanos não atendem a contento as especificidades do público adolescente.

Abstract We aimed to analyze the geographic distribution, the structure of healthcare services and the human resources of all units of the Sistema Único de Saúde (SUS - the Unified Health System) that provide sexual and reproductive health (SRH) services to the adolescent population in the second largest city in Brazil. We conducted a cross-sectional study with geographical mapping and data collection through a questionnaire applied in person with coordinators of the units or their representatives in 147 outpatient clinics in Rio de Janeiro that have SSR services. We found that in over 90% of the units, adolescents are treated together with the adult population, without particular shifts or rooms for this age group. In more than 10% of services, treatment is only provided with the presence of the guardian. In cases of sexual violence, this proportion is 34%. Specific educational activities for this age group are only carried out in 12.9% of units and less than one third of doctors had received some kind of training to deal with adolescent health. In conclusion, despite the wide geographic distribution of health facilities, the structure of care and the human resources do not meet the specific needs of adolescents.