Abstract Sperm motility and kinematics analysis are important to predict bull fertility. However, there are other molecules in the sperm with the ability to improve the pregnancy rate. For example, PLCZ1 is a sperm protein that plays a unique role in the activation of the zygote and is important for the survival of the embryo. The objective of this work was to compare the expression of PLCZ1 mRNA in sperm cells of Chihuahuan Criollo and European bulls in the winter and summer seasons, under a low-input system. Six (3.33 ± 0.43 years old) bulls (three Criollo, three European) were used. Gross and individual motility were measured in semen obtained by electrostimulation. The cell pack was pelletized by centrifugation and stored in liquid nitrogen. The sperm cells were purified and total RNA was extracted. cDNA was synthesized to perform qPCR and measure the relative level of PLCZ1 transcripts in each bull. There were no differences in individual motility, however, gross motility was lower (P < 0.05) in Criollo bulls, both in the winter (71.1 ± 2.8 vs. 76.6 ± 2.8%) and in the summer season (58.9 ± 2.8 vs. 77.7 ± 2.8%). PLCZ1 expression was 5.3 times higher (P < 0.05) in winter than in summer (5.09 ± 1.09 vs 0.959 ± 1.09). No difference (P>0.05) was found in the expression levels of PLCZ1 between both breeds (4.36 ± 1.09 vs 1.69 ± 1.09), for Criollo and European, respectively. Although the animals presented seminal motility within the recommended limits for insemination, the expression levels of PLCZ1 vary depending on the time of the year and this might impact the rate of successful pregnancies. Therefore, it is important to complement conventional analysis of seminal quality with molecular characteristics.

Abstract Avian pox can severely impact turkey production systems. Vaccination programs in Mexico use commercially available Fowlpoxvirus vaccines, that are used across different bird species. Nonetheless, there are reports of sporadic disease outbreaks among vaccinated turkeys, which suggest that heterologous vaccines may provide limited immunity, presenting the need to develop homologous vaccines that can better protect turkeys. This study compared the protection granted to turkey chicks by a commercial Fowlpoxvirus vaccine and by a live attenuated Turkeypoxvirus vaccine after a challenge with a field isolated Turkeypoxvirus virus. Histopathology, polymerase chain reaction, and sequencing of DNA were used for viral identification. A Turkeypoxvirus strain was first isolated in chicken embryo lesions, and subsequently adapted through serial passes in chorioallantoic membrane to produce the homologous vaccine. The attenuated virus was used as a vaccine when a 104.4 embryo ID50/mL titre was reached. Three groups of three-week-old turkey chicks were used for challenge experiments. Subjects in Group 1 were immunized with the attenuated Turkeypoxvirus vaccine (homologous vaccine). Chicks in Group 2 were vaccinated with the commercially available heterologous vaccine (Fowlpoxvirus). Subjects in Group 3 were not vaccinated and received only saline solution (control group). Two weeks after vaccination, animals from Group 1 reached a 97.7 ND50 seroneutralization titre, while levels reached in Group 2 birds and in control chicks were 11.7 ND50 (Group 2) and zero, respectively. At this time, all groups were challenged with a suspension of a field-isolated Turkeypox virus. The homologous vaccine afforded 100% protection in Group 1 (10/10 individuals), while only 10% (1/10) of individuals in Group 2 were protected by the commercial heterologous Fowlpoxvirus vaccine. None of the non-immunized birds in Group 3 were protected (0/10). These results show that the homologous vaccine afforded a greater protection against a Turkeypox virus infection than that observed for the heterologous vaccine, and that a homologous vaccine can be efficiently produced by isolating and attenuating the virus from turkeypox lesions, through chorioallantoic membrane serial passes.

Abstract The aim of the present study was to determine the floristic composition of the sarcocaule scrub of the Sonoran desert in an area of 800 ha within an ejidal property located in the limits of the city of La Paz, Southern Baja California. Sampling was carried out during the rainy season (September-November 2018) and at the end of the dry season (May-August 2019), using 20 quadrants of 5 m × 5 m. 29 species of 1 634 individuals were identified, between the two seasons and the variables of height and crown diameter of each specimen by species were considered, in order to determine abundance (A r ), dominance (D r ), frequency (F r ), importance value index (IVI) and the Shannon index for species diversity (H´) and the Margalef index for species richness (S´). During the rainy season, Jatropha cinerea is observed as the dominant species D r = 7.15 % and with the highest IVI (10.7 %) and Turnera diffusa as the most abundant with A r = 3.2. On the other hand, for the dry season, Prosopis articulata is the dominant species D r = 9.29 and with the highest IVI (12.51 %) and Mammillaria armillata as the most abundant, with A r = 4.56. The indices of H' and S' during the rainy season were 3.36 and 8.09, respectively, with a difference between them (p≤0.05) when compared with those of the dry season (2.88 and 4.88, respectively), due to the absence of species and decrease in the number of individuals, than in the wet season.

Resumen El objetivo del presente estudio fue determinar la composición florística del matorral sarcocaule del desierto sonorense en un área de 800 ha dentro de una propiedad ejidal ubicada en los límites de la ciudad de La Paz, Baja California Sur. El muestreo se realizó durante la época de lluvias (septiembre-noviembre de 2018) y a finales de la épocade sequía (mayo-agosto de 2019), por medio de 20 cuadrantes de 5 m × 5 m. Se identificaron 29 especies de 1 634 individuos, entre las dos temporadas y se consideraron las variables de altura y diámetro de copa de cada ejemplar por especie, para determinar abundancia (A r ), dominancia (D r ), frecuencia (F r ), índice de valor de importancia (IVI) y los índices de Shannon para la diversidad de especies (H´) e índice de Margalef para la riqueza de especies (S´). Durante la época de lluvias, se observó a Jatropha cinerea como dominante D r =7.15 % y con mayor IVI (10.7 %); Turnera diffusa fue la más abundante con A r =3.2. En cambio, para la temporada seca, fue Prosopis articulata la especie dominante D r =9.29 y con mayor IVI (12.51 %); Mammillaria armillata fue la más abundante, con A r =4.56. Los índices de (H’) y (S’) durante la época de lluvias fueron 3.36 y 8.09, respectivamente, con diferencia entre ellos (p≤0.05) al compararlos con los de la temporada seca (2.88 y 4.88, respectivamente), debido a la ausencia de taxones y disminución del número de individuos, en relación con la época húmeda.

Resumen Se presenta un caso de una paciente de 69 años de edad y sin antecedentes personales o familiares de patología tiroidea alguna, que asiste con un estado convulsivo de aparición súbita, de hemicuerpo derecho y relajación esfinteriana, asociado, además, a trastornos del estado de conciencia, bradicardia, bradipnea y edema de difícil Godet en miembros inferiores. Los exámenes complementarios mostraron acidosis respiratoria, hiposecreción de T4 y elevación de la hormona estimulante de la tiroides por retroalimentación negativa. Estos resultados corroboraron el diagnóstico presuntivo de coma mixedematoso. Se emprendió el tratamiento hormonal correspondiente con levotiroxina, al cual respondió satisfactoriamente y fue egresada a los 24 días de su ingreso, pendiente de valoración por el especialista de endocrinología.

Abstract We present the case of a 69-year-old female patient with no personal or family history of any thyroid disease, who attends with a convulsive state of sudden onset, right hemibody and sphincter relaxation, also associated with disorders of the state of consciousness, bradycardia, bradypnea and edema of difficult godet in lower limbs. Complementary analyses showed respiratory acidosis, hyposecretion of T4 and elevation of the thyroid stimulating hormone by negative feedback. These results corroborated the presumptive diagnosis of myxedema coma. The corresponding hormonal treatment with levothyroxine was undertaken, to which it responded satisfactorily and was discharged 24 days after admission, pending evaluation by the endocrinology specialist for an outpatient hormone replacement therapy.

Abstract: Tuberculosis is typically considered a chronic disease, although it can be manifested acutely, whose form is reviewed in this clinical case. The pathogenesis of acute tuberculosis is usually related to epidemiological and genetic factors of the host. Septic shock due to Mycobacterium tuberculosis is rare, but it is well recognized, behaving similarly to bacterial septic shock, known as sepsis tuberculosa gravissima. Our patient was admitted with hemodynamic compromise and diagnosis of septic shock that evolved to multiorganic failure; despite antimicrobial and antituberculous management, patient died five days after admission to the intensive care unit. The spectrum of M. tuberculosis disease makes diagnosis difficult, unless clinical suspicion is high, but high mortality requires consideration. The early start of therapy is decisive to optimize the clinical outcome.

Resumen: La tuberculosis típicamente se considera una enfermedad crónica, aunque puede manifestarse de forma aguda, cuya manifestación se revisa en este caso clínico. La patogenia de la tuberculosis aguda suele estar relacionada con factores epidemiológicos y genéticos del huésped. El choque séptico debido a Mycobacterium tuberculosis es poco frecuente, pero está bien reconocido, se comporta de manera similar al choque séptico bacteriano, conocido como sepsis tuberculosa gravissima. Nuestra paciente ingresó con afectación hemodinámica y diagnóstico de choque séptico que evolucionó a insuficiencia multiorgánica; a pesar del tratamiento antimicrobiano y antituberculoso establecido, falleció a los cinco días del ingreso a la unidad de cuidados intensivos. El espectro de la enfermedad por M. tuberculosis dificulta el diagnóstico, a menos que la sospecha clínica sea alta, pero la alta mortalidad exige su consideración. El inicio temprano del tratamiento es decisivo para optimizar el resultado clínico.

ABSTRACT This investigation is part of the studies carried out by the Faculty of Technical Sciences, the Agricultural Mechanization Center (CEMA) of the Agrarian University of Havana (UNAH) and the Institute of Animal Science (ICA), on the evaluation of agricultural assemblies for the production of animal feed. It was developed with the objective of determining the energy costs of the Russian BELARUS 510 Tractor and of the Brazilian JF-50 forage chopper. It is determined that the total energy costs depend, to a greater extent, on the energy sequestered in fuel (ESc), with a value of 103,73 MJ/h and 53% as the highest value. The energy sequestered in lubricants and filters (ESl) represented the lowest dependency with a value of 5,19 MJh, being 2,65% the lowest value. Total hourly energy costs (EST) amount to 195,48 MJ/h and energy costs total per unit mass processed (ESt) to 365 MJ/t.

RESUMEN Como parte de los estudios realizados por la Facultad de Ciencias Técnicas, el Centro de Mecanización Agropecuaria (CEMA) de la Universidad Agraria de la Habana (UNAH) y el Instituto de Ciencia Animal (ICA), sobre la evaluación de conjuntos agrícolas para la producción de alimento animal, se desarrolló esta investigación cuyo objetivo consiste en determinar los costos energéticos del Tractor BELARUS 510 de origen ruso y la Picadora de Forraje JF-50 de origen brasileño. Se determina que los costos energéticos totales dependen en mayor medida de la energía secuestrada en combustible (ESc) con un valor de 103,73 MJ/h, siendo la de mayor porcentaje con 53% y la energía secuestrada en lubricantes y filtros (ESl) en menor medida con un valor de 5,19 MJ/h, siendo la de menor porcentaje con 2,65%, los costos energéticos horarios totales (EST) ascienden a 195,48 MJ/h y los costos energéticos totales por unidad de masa procesada (ESt) a 365 MJ/t.

ABSTRACT This manuscript introduces Wastewater-Based Epidemiology (WBE) and its potential in the assessment of diverse aspects related to public health. This methodology can provide data in a relatively short temporal and local scale (typically dialy-weekly at the municipal level) on consumption patterns of illicit drugs (e.g. cocaine or cannabis), licit substances of abuse (e.g. alcohol) by measuring their consumption biomarkers (i.e. the original unmetabolized substance or some of its metabolite) in wastewater. Besides discussing the fundaments, advantages and shortcomings of WBE, it reviews some of the main precedents at international level and most remarkable activities that have been taken place in this field in Spain. Finally, the Spanish Network of Wastewater-Based Epidemiology (ESAR-Net) as is presented. ESAR-Net is an Excellence Network that sums up the efforts of the most relevant Spanish researchers in the field of WBE, aiming to investigate future perspectives of this methodology and its impact on Public Health competences in Spain.

RESUMEN En este artículo se presenta la metodología de análisis de aguas residuales con fines epidemiológicos (wastewater-based epidemiology, WBE) y su potencial para abordar diversos aspectos relacionados con la salud pública. Esta metodología permite obtener datos a una escala temporal y espacial relativamente pequeña (típicamente datos diarios-semanales sobre un municipio) de hábitos de consumo de sustancias de abuso, ilegales (como la cocaína o el cannabis) o legales (como el alcohol) a través de la determinación de biomarcadores de consumo (el compuesto original no metabolizado o alguno de sus metabolitos) en el agua residual. Aparte de discutir los fundamentos, ventajas y limitaciones de WBE, se comentan los precedentes más relevantes a nivel internacional, y las actividades más destacables en España en este ámbito. Finalmente, se exponen, los objetivos de la Red Española de Análisis de Aguas Residuales con Fines Epidemiológicos (ESAR-Net), una “Red de Excelencia” que agrupa a investigadores españoles con amplia experiencia en el área de WBE, así como las perspectivas de futuro de esta metodología puede tener para mejorar las competencias de la Salud Pública en España.

ABSTRACT This manuscript introduces Wastewater-Based Epidemiology (WBE) and its potential in the assessment of diverse aspects related to public health. This methodology can provide data in a relatively short temporal and local scale (typically dialy-weekly at the municipal level) on consumption patterns of illicit drugs (e.g. cocaine or cannabis), licit substances of abuse (e.g. alcohol) by measuring their consumption biomarkers (i.e. the original unmetabolized substance or some of its metabolite) in wastewater. Besides discussing the fundaments, advantages and shortcomings of WBE, it reviews some of the main precedents at international level and most remarkable activities that have been taken place in this field in Spain. Finally, the Spanish Network of Wastewater-Based Epidemiology (ESAR-Net) as is presented. ESAR-Net is an Excellence Network that sums up the efforts of the most relevant Spanish researchers in the field of WBE, aiming to investigate future perspectives of this methodology and its impact on Public Health competences in Spain.

RESUMEN En este artículo se presenta la metodología de análisis de aguas residuales con fines epidemiológicos (wastewater-based epidemiology, WBE) y su potencial para abordar diversos aspectos relacionados con la salud pública. Esta metodología permite obtener datos a una escala temporal y espacial relativamente pequeña (típicamente datos diarios-semanales sobre un municipio) de hábitos de consumo de sustancias de abuso, ilegales (como la cocaína o el cannabis) o legales (como el alcohol) a través de la determinación de biomarcadores de consumo (el compuesto original no metabolizado o alguno de sus metabolitos) en el agua residual. Aparte de discutir los fundamentos, ventajas y limitaciones de WBE, se comentan los precedentes más relevantes a nivel internacional, y las actividades más destacables en España en este ámbito. Finalmente, se exponen, los objetivos de la Red Española de Análisis de Aguas Residuales con Fines Epidemiológicos (ESAR-Net), una “Red de Excelencia” que agrupa a investigadores españoles con amplia experiencia en el área de WBE, así como las perspectivas de futuro de esta metodología puede tener para mejorar las competencias de la Salud Pública en España.

As part of the studies carried out by the Technical Sciences Faculty, the Agricultural Mechanization Center (CEMA) of the Havana Agricultural University (UNAH) and the Animal Science Institute (ICA), on the agricultural groups evaluation for the production of animal food, with the objective the energy cost to determine of the forage chopper machine MF IIMA model EM - 01 modified of national production, with electric working, an researching was developed. It is determined that the totals energy costs depend in bigger measure of the kidnapped energy in electricity (ESe) with a value of 17, 23 MJ/h, for the percentage biggest of 64% and the kidnapped energy in materials, production and transport (ESm) presented the small dependence with a value of 2,25 MJ/h for a percentage of 8,35%; the costs energy totals schedules (EST) of the forage chopper MF IIMA model EM-01 modified ascends at 26,93 MJ/h and the total energy costs for unit of processed mass (ESt) at 84,15 MJ/t

Como parte de los estudios realizados por la Facultad de Ciencias Técnicas, el Centro de Mecanización Agropecuaria (CEMA) de la Universidad Agraria de la Habana (UNAH) y el Instituto de Ciencia Animal (ICA), sobre la evaluación de conjuntos agrícolas para la producción de alimento animal, se desarrolló esta investigación, cuyo objetivo consiste en determinar los costos energéticos de la picadora de forraje MF IIMA modelo EM- 01 perfeccionada, de fabricación nacional,con accionamiento eléctrico. Se determina que los costos energéticos totales dependen en mayor medida de la energía secuestrada en electricidad (ESe) con un valor de 17,23 MJ/h, para el mayor porcentaje de 64% y la energía secuestrada en materiales, fabricación y transporte (ESm) representó la menor dependencia con un valor de 2,25 MJ/h para un porcentaje de 8,35%; los costos energéticos horarios totales (EST) de la picadora MF IIMA modelo EM-01 perfeccionada ascienden a 26,93 MJ/h y los costos energéticos totales por unidad de masa procesada (ESt) a 84,15 MJ/t

Abstract: A computer platform was developed for integrated irrigation scheduling and management of potato crops based on information and communications technology using the internet. This technology adjusted the water demands of the potato crop according to the climate variability observed over recent years in the Valle del Fuerte, Sinaloa, Mexico. The basis of the technology was an integrated irrigation scheduling model based on increasing degree-days, with the integration of Irrimodel software operated over the internet. The system has the following capabilities: a) generates irrigation plans based on different scenarios related to climate, water availability and water management; b) produces a highly accurate irrigation prognosis according to the development of the crop and the accumulation of degree-days, and c) generates and sends irrigation requests to the service providers. The technology was transferred to producers over three months and covered over 3 000 ha. Two levels of use were identified -a high application level by 56% of the area (1 738 ha) and a medium level by the remaining area. The area with a high application level was composed of 396 ha which used gravity irrigation and 1 334 ha which used sprinkler irrigation. In both areas, with gravity irrigation as well as sprinkler irrigation, there was an increase in application efficiency (18%), yield (7 t) and water productivity (1.5 kg·m-3). The increase was greater with gravity than with traditional management. This work presents an analysis of the use of this technology on a large scale, as an option to adapt irrigation scheduling to the effects of climate change.

Resumen: Se desarrolló una plataforma computacional basada en tecnologías de información y comunicación (TIC´s) para la programación integral y gestión de riego por Internet en el cultivo de papa, la cual ajusta las demandas hídricas del cultivo de papa a la variabilidad climática observada en los últimos años en el Valle del Fuerte, Sinaloa, México. El fundamento de la tecnología fue un modelo de programación integral del riego basado en grados-día crecimiento (GD), integrándose a un software (Irrimodel) operado a través de Internet. El sistema cuenta con las siguientes capacidades: a) elabora planes de riego bajo diferentes escenarios climáticos, disponibilidad de agua y manejo; b) pronostica el riego con alta precisión de acuerdo con el desarrollo del cultivo y acumulación de GD; y c) genera y envía solicitudes de riego al módulo prestador del servicio. La transferencia a productores se hizo en tres meses en más de 3 000 ha, donde se presentaron dos niveles de uso. En 56% (1 738 ha), su aplicación fue con nivel alto, mientras que el resto con nivel medio. La superficie con nivel alto se conformó de 396 ha bajo riego por gravedad y 1 334 ha bajo riego por aspersión. Tanto en parcelas con riego por gravedad como de aspersión se incrementó la eficiencia de aplicación (Ea) (18%), rendimiento (7 t) y productividad del agua (1.5 kg·m-3), siendo mayor en las de gravedad contra manejo tradicional. En este trabajo se presenta un análisis de esta tecnología a gran escala como alternativa para la adaptación de la programación del riego ante los efectos del cambio climático.

This research aims to identify the volatile compounds of genus Origanum. For this purpose 50 samples was collected locations: Porobaya, San June, Otora, Doce Quebradas and Tala; belonging at district of Torata-Moquegua; Samples collected were characterized taxonomically finding four species Origanum majoricum (compact and loosely), Origanum majorana and Origanum plegatifolium. Subsequently the volatiles were analyzed by solid phase microextraction (SMPE) and GC-MS. 31 compounds were identified by comparison with literature, co-elution with standard and comparing retention indices, finding that the content of thymol and carvacrol range from 7 to 14 mg/g and 37 to 49 mg/g respectively. Porobaya samples and San June are increasing the amount of thymol present; Otora samples being more carvacrol content. Therefore the SMPE turns out to be a quick and easy method for identifying volatile aromatic plants.

El presente trabajo de investigación tuvo por finalidad identificar los compuestos volátiles del género Origanum. Para tal fin se recolectó 50 muestras de las localidades de Porobaya, San June, Otora, Doce quebradas y Tala; pertenecientes al distrito de Torata-Moquegua; las muestras recolectadas fueron caracterizadas taxonómicamente encontrándose tres especies Origanum majoricum (forma compacta y laxa), Origanum majorana y Origanum plegatifolium. Posteriormente, se analizó los compuestos volátiles mediante microextracción en fase sólida (SMPE) y GC-MS. Se identificaron 31 compuestos por comparación con la literatura, co-elución con estándares y comparación de índices de retención, encontrándose que el contenido de timol y carvacrol varían de 7 a 14 mg/g y de 37 a 49 mg/g, respectivamente. Las muestras de Porobaya y San June presentaron la mayor cantidad de timol; mientras que Otora presentó el mayor contenido de carvacrol. Por lo cual la SMPE resulta ser un método rápido y sencillo para identificar volátiles en plantas aromáticas.

In the experimental farm of the Facultad de Ciencias Agropecuarias y Forestalesde of the Universidad Nacional de Itapúa, Natalio headquarters, a research was conducted with the objective of studying the response of transgenic cotton variety NuOpal (BGRR) seeded at different row spacing and distance between plant, from November 2012 to April 2013. The studied treatments were arranged in a split plot, were main plot being the distance between plants (10 and 20 cm) and subplots row spacing (38, 50, 75 and 100 cm) in a complete block design with four replications. The variables evaluated were: beginning of flowering, plant height, stem diameter, number of fruiting branches, number of ball, number of seed cotton, weight of seed cotton and seed cotton yield per plant. Data was analyzed using ANAVA and the variables with significant differences were adjusted by regression equations. Significant differences were observed for all variables except the beginning of flowering. The best yields were observed when distance between plants were 20 cm (3,394 kg/ha) and 38 cm between rows (3,580 kg/ha), decreasing linearly with increasing row spacing.

En la finca experimental de la Facultad de Ciencias Agropecuarias y Forestales de la Universidad Nacional de Itapúa, sede Natalio, se realizó una investigación con el objetivo de estudiar la respuesta del algodonero de la variedad transgénica NuOpal (BGRR) a la siembra en diferentes espaciamientos entre surcos y entre plantas, durante el periodo agrícola de noviembre 2012 a abril 2013. Los tratamientos bajo estudio fueron distribuidos en un arreglo de parcelas divididas siendo la parcela principal el distanciamiento entre plantas (10 y 20 cm) y las subparcelas distancia entre hileras (38, 50, 75 y 100 cm) en un diseño de bloques completos al azar con cuatro repeticiones. Las variables evaluadas fueron: inicio de floración, altura de planta, diámetro del tallo, número de ramas fructíferas, número de cápsula, número de capullos, peso del capullo y rendimiento de algodón en rama. Para el procesamiento de datos se realizó el análisis de varianza, y las variables que resultaron con diferencias significativas fueron ajustadas a una ecuación de regresión. Se observaron diferencias significativas para todas las variables, excepto en inicio de floración. Los mejores rendimientos fueron observados en la distancia entre plantas de 20 cm (3.394 kg/ha) y 38 cm entre hileras (3.580 kg/ha), decreciendo en forma lineal al aumentar el espacio entre hileras.

Glucose and related hexoses are very important metabolic substrates. Their most important function is to provide quick fuel for most organisms in all three kingdoms because they are the first substrate for energy production in the form of ATP through glycolysis and the subsequent metabolic pathways. In this paper we review the current information about how glucose and related hexoses are transported across biological membranes to carry out their function either as a metabolic molecule or as energy store in marine invertebrate organisms. In these animals, there are two sugar transport systems that are mediated by the sodium/solute symporter family proteins (SGLT) and the major facilitative super-family proteins (GLUT). The most studied sugar transporters in marine invertebrates are involved with dietary sugar uptake, such as SGLT1, SGLT4, GLUT2 and GLUT5, however more studies need to be done to extend the knowledge about these and other sugar transporters involved in metabolic processes.

This article reviews the dimensions of service quality perceived by customers of the electric system for research through a model, which integrates the theories of SERVQUAL, outcome quality and field service. Factor analysis to evaluate the composition of the dimensions and a structural equation method to validate the theoretical model of the case were applied. The sample is of 3,803 users via a telephone survey operated in the states of Sonora and Sinaloa in the Mexican Republic. The factor analysis results show that the composition of the dimensions of functional service quality or process does not match the composition and dimensions of the SERVQUAL model. Likewise, the construct of the functional quality in base is different from the construct of the functional quality in field and, finally, the structural equation analysis confirms that the construct of outcome quality is not a separate dimension from the functional quality, therefore, both measure service quality.

En este artículo se revisa las dimensiones de la calidad del servicio percibida por los clientes del sistema eléctrico para una investigación a través de un modelo, integrando las teorías de SERVQUAL, calidad de salida y servicio en campo. Así, se aplicó un análisis factorial para evaluar la composición de las dimensiones y el método de ecuaciones estructurales para validar el modelo teórico del caso. La muestra es de 3 803 usuarios de los estados de Sonora y Sinaloa de la República Mexicana, la cual se operó por medio de una encuesta telefónica. Los resultados en el análisis factorial mostraron que la composición de las dimensiones de la calidad del servicio funcional o de proceso no coincide con la composición y dimensiones del modelo SERVQUAL; asimismo, el constructo de la calidad funcional en base es diferente al constructo de la calidad funcional de campo y, por último, el análisis de ecuaciones estructurales confirma que el constructo de la calidad de salida no es una dimensión diferente separada de la calidad funcional, por lo que ambas miden la calidad del servicio.

A investigação fitoquímica das partes aéreas de Centaurea omphalotricha levou ao isolamento de três lactonas sesquiterpênicas novas, 4'-acetilcinaropicrina, 4'-acetilcebelina F e 15-acetil desidromelitensina, juntamente com doze compostos conhecidos, sendo sete lactonas sesquiterpênicas, dois isoprenóides e três flavonóides. As estruturas dos novos compostos foram elucidadas por meio de RMN 1D e 2D, e espectrometria de massas, e por comparação com os dados descritos na literatura. O efeito de lactonas sesquiterpênicas sobre a viabilidade das células tumorais humanas, linhagens HL-60 e U937, também foi investigado e 3-acetilcinaropicrina e 4'-acetilcinaropicrina foram os compostos mais citotóxicos contra células de leucemia humana com valores de IC50 de 2,0 ± 0,9 e 5,1 ± 0,4 µmol L-1, respectivamente.

Phytochemical research of the aerial parts of Centaurea omphalotricha led to the isolation of three new sesquiterpene lactones, 4'-acetyl cynaropicrin, 4'-acetyl cebellin F and 15-acetyl dehydromelitensin, together with twelve known compounds, seven sesquiterpene lactones, two isoprenoids and three flavonoids. The structures of the new compounds were elucidated by means of extensive 1D and 2D NMR, and MS, and by comparison with reported data in the literature. The effect of sesquiterpene lactones on the viability of the human tumor cell lines HL-60 and U937 was also investigated and 3-acetyl cynaropicrin, and 4'-acetyl cynaropicrin were found to be the most cytotoxic compounds against human leukemia cells with an IC50 values of 2.0 ± 0.9 and 5.1 ± 0.4 µmol L-1, respectively.